Tesis sobre el tema "Gene expression profiling"
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Muszynska, Dorota. "Gene expression profiling in Keratoconus". Thesis, Queen's University Belfast, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.602693.
Texto completoLeonard, Pauline Catherine. "Gene expression profiling of osteosarcoma". Thesis, University College London (University of London), 2006. http://discovery.ucl.ac.uk/1445814/.
Texto completoHägg, Sara. "Gene Expression Profiling of Human Atherosclerosis". Doctoral thesis, Linköpings universitet, Biologiska Beräkningar, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-52085.
Texto completoBain, Peter A. y n/a. "Gene Expression Profiling of Cylindrospermopsin Toxicity". Griffith University. School of Biomolecular and Physical Sciences, 2007. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20080404.145834.
Texto completoBeijnum, Judith Rosina van. "Gene expression profiling of tumor angiogenesis". Maastricht : Maastricht : Universiteit Maastricht ; University Library, Maastricht University [Host], 2006. http://arno.unimaas.nl/show.cgi?fid=7710.
Texto completoAl-Halabi, Hani. "Gene expression profiling in adult Medulloblastoma". Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=107616.
Texto completoLe médulloblastome (MB) représente environ 1% des tumeurs du cerveau adulte, et en tant que telle est une maladie peu étudiée. Il présente différentes manifestations cliniques et differents résultats chez les adultes, mais il est encore traité selon les protocoles pédiatriques. Récemment, des études d'expression de profil génétique suggére l'existence d'au moins 4 sous-types moléculaires pour le MB pédiatriques, caractérisées par des profils génétiques distincts, par l'activation des voies de signallisation oncogéniques et par des résultats cliniques variées. Cela a amélioré notre capacité de classifier le MB, a mieux sélectionné les tumeurs pour le traitement ciblé et ouvre la voie à une médecine individualisée. On ignore si le MB adulte possede une signature moléculaire unique et si ce sont des différences biologiques qui expliquent les différences cliniques observées dans la population adulte. Nous proposons que le MB possede des différences biologiques qui varie en fonction de l'âge et afin de mieux caractériser ces différences, nous avons étudié les profils d'expression génetique d'une cohorte de MB adultes et les ont comparés à une base de données de MB pédiatrique. Nous avons trouvé une prépondérance d'activation de la voie SHH parmi les tumeurs de MB adulte (26/31, 84%). Ceci supporte les données qui ont identifié la signature SHH dans 75% des MB adultes. Compte tenu des rapports sur la bonne réponse clinique observée avec l'utilisation d'inhibiteurs sélectifs de Shh dans le MB à SHH, nos résultats suggèrent que l'utilisation adjuvante de ces agents peut améliorer les résultats pour le MB adulte et devrait être étudiée dans une étude prospective. Le MB adulte avec Sonic hedgehog actif représente une population homogène de tumeur, et présente des différences dans les profils moléculaires par rapport au MB pédiatriques à SHH. Cela pourrait expliquer les résultat variable entre adultes et enfants avec le MB à SHH, et décrit des cibles potentielles de la tumorogenèse du MB chez les adultes. Cette thèse propose de nouveaux aperçus sur l'effet de l'âge sur les différences moléculaires pour le MB et met en évidence que les patients adultes representent des cibles potentielles pour la thérapie de ciblage sélectif de la voie SHH.
Goh, Huey Tse. "Profiling cardiac gene expression during endotoxaemia". Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523087.
Texto completoBrooks, W. M. "Profiling gene expression in Alzheimer's disease". Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596943.
Texto completoCleator, Susan Jane. "Gene expression profiling of breast cancers". Thesis, Institute of Cancer Research (University Of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423120.
Texto completoBain, Peter A. "Gene Expression Profiling of Cylindrospermopsin Toxicity". Thesis, Griffith University, 2007. http://hdl.handle.net/10072/367068.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Physical Sciences
Faculty of Science, Environment, Engineering and Technology
Full Text
Paon, Lenaic Marie Pierre. "Gene expression profiling of metastatic gastric cancer". Thesis, University of Liverpool, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490659.
Texto completoQuinn, Michael Corwin James y n/a. "Gene expression profiling of human granulosa cells". University of Otago. Department of Anatomy & Structural Biology, 2005. http://adt.otago.ac.nz./public/adt-NZDU20070501.144002.
Texto completoGeatrell, Jenny. "Apoptosis gene expression profiling in lens development". Thesis, Cardiff University, 2007. http://orca.cf.ac.uk/54644/.
Texto completoDennis, Jayne L. "Gene expression profiling and classification of adenocarcinoma". Thesis, University of Glasgow, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411836.
Texto completoSharp, Margaret. "Global gene expression profiling of canine lymphoma". Thesis, Sharp, Margaret (2017) Global gene expression profiling of canine lymphoma. PhD thesis, Murdoch University, 2017. https://researchrepository.murdoch.edu.au/id/eprint/38017/.
Texto completoGould, Barbara. "The genomics of labour : global gene expression profiling and oxytocin receptor gene expression". Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84251.
Texto completoThe oxytocin receptor (OTR) gene encodes one such CAP. Northern blot and real-time RT-PCR demonstrated its up-regulation prior to labour in each model, preferentially in normal labour. Uterine contraction promotes increased central and peripheral oxytocin release and synaptic plasticity. To further examine the role of the OTR, we developed an OTR-lacZ reporter mouse. We mapped, by X-gal histochemistry, the distribution of OTR gene expression in the early postparturient mouse brain and identified novel regions of expression. These included the piriform cortex, entorhinal cortices, and parasubiculum, which support memory function. Dorsal tegmental, vestibular, and lateral reticular nuclei expression suggests the transmission of locomotor inputs. Hypoglossal, facial, and spinal trigeminal nuclei support maternal behaviours. We also more accurately demarcated OTR gene expression in the solitary tract nucleus responsible for relaying contraction stimulation of oxytocin release.
These studies provide a more accurate knowledge base for the development of successful therapies to decrease the incidence of premature labour.
Liu, Zhilin. "Gene expression profiling of bovine ovarian follicular selection". Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/4490.
Texto completoThe entire dissertation/thesis text is included in the research.pf file; the official abstract appears in the short.pf file (which also appears in the research.pf); a non-technical general description, or public abstract, appears in the public.pf file. Title from title screen of research.pf file (viewed on May 6, 2009) Vita. Includes bibliographical references.
Smith, Nolan R. "Gene Expression Profiling in Heat Stressed Scaphirhynchus Sturgeon". OpenSIUC, 2020. https://opensiuc.lib.siu.edu/theses/2759.
Texto completoArlinde, Christina. "Gene expression profiling in animal models of alcoholism /". Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-133-4/.
Texto completoDhoogra, Minishca. "Gene expression profiling of polyamine-depleted Plasmodium falciparum". Diss., Pretoria : [s.n.], 2007. http://upetd.up.ac.za/thesis/available/etd-12132007-103643/.
Texto completoHughes, B. "Gene expression profiling of quiescent peripheral T lymphocytes". Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604742.
Texto completoWarner, Giles C. "Gene expression profiling of head and neck cancer". Thesis, Queen Mary, University of London, 2004. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1857.
Texto completoHime, Audrey Elise. "Gene expression-based profiling for studying dystonia pathogenisis". Thesis, Boston University, 2012. https://hdl.handle.net/2144/12421.
Texto completoThis project mainly focuses on DYT6 dystonia and its causative gene: THAP1, which encodes a proposed transcription factor, THAP1, which exhibits sequencespecific and zinc-dependent DNA-binding activity. Over 50 pathogenic mutations have been identified in THAP1, and various clinical manifestations of DYT6 have been reported. The target genes of THAP1 are not fully understood, although some genes involved in cell cycle regulation have been shown to respond transcriptionally to changes in THAP1 levels. While a connection between THAP1 and cellular proliferation has been suggested, the exact relationship is still unclear, and there is an on-going interest in finding out more about the normal function(s) of THAP1 and how these functions are affected by DYT6 mutations. It can be difficult to interpret results from gene expression profiling to further understand a protein's biochemical activities and its basis for pathogenesis. Therefore this project uniquely inputs DYT6 gene expressionbased profiling data into the Connectivity Map, a signature profile reference database and software matching system, to identify known compounds that predict similar and opposite biological signatures as patients with DYT6. The results of this CMAP query show that compounds with strong positive scores, including HDAC inhibitors, HSP90 inhibitors, and phenothiazines, all are associated in the literature with antiproliferative effects. Compounds with strong negative scores show evidence of promoting cell cycle re-entry as well as neurogenesis, which may be considered to be part of a reverse signature of decreased proliferation. Hence bioinformatics analysis may provide clues to THAP1 and its biochemical mechanism of action, specifically in relation to proliferation and the cell cycle.
Nathanson, Jason Lawrence. "Cell type specific gene expression profiling and targeting /". Diss., View abstract only; access to full text of dissertation for UC campuses will be available after September 1, 2010, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p3320635.
Texto completoTitle from first page of PDF file (viewed September 24, 2008). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 135-150).
Ståhlberg, Nina. "Gene expression profiling in molecular studies of hormone actions /". Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-706-1/.
Texto completoLam, Chi-leung David. "Gene expression profiling in non-small cell lung cancer". Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B38585777.
Texto completoTan, Wei Min. "Single cell gene expression profiling of human epidermal keratinocytes". Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609685.
Texto completoMulaw, Medhanie Assmelash. "Early target genes of CALM/AF10 as revealed by gene expression profiling". Diss., kostenfrei, 2009. http://edoc.ub.uni-muenchen.de/10480/.
Texto completoForsman, M. (Minna). "Histological characteristics and gene expression profiling of Dupuytren’s disease". Doctoral thesis, Oulun yliopisto, 2016. http://urn.fi/urn:isbn:9789526211671.
Texto completoTiivistelmä Kämmenkalvon kuroumatauti eli Dupuytrenin kontraktuura on valkoihoisen miehen kämmenkalvon sairaus. Sairastumisen todennäköisyys lisääntyy ikääntymiseen liittyen, mutta vahva sukurasitus poikkeuksellisesti altistaa sairaudelle jo tavanomaista nuoremmalla iällä. Myofibroblastit ovat tärkein ja edustetuin solutyyppi Dupuytren kudoksessa. Huolimatta runsaasta tutkimustyöstä ei etiologiaa ole saatu vielä selvitettyä. Sukurasitus näyttää selkeästi altistavan taudille. Toistaiseksi kyetään hoitamaan ainoastaan sairauden aiheuttamat seuraukset, mutta ei perussyytä. Lisäksi tauti uusiutuu. Dupuytrenin sukurasitus lisää uusiutumista suurella todennäköisyydellä. Myös uusiutumisaika on tuolloin tavanomaista nopeampi, ja kyseessä katsotaan olevan ns.aggressiivisempi taudin muoto. Väitöskirjatyössäni pyrittiin löytämään mahdollisia tekijöitä, joiden perusteella voitaisiin ennustaan onko kyseessä aggressiivisempi vai tavanomainen taudin muoto. Tätä varten tutkittiin kaksikymmentä yksi Dupuytren kudosnäytettä ja viisi tervettä kämmenkalvon näytettä immunohistologisilla värjäyksillä, ja voitiin todeta, että soluisuus oli selkeästi koholla aggressiivisten ja taudin uusineiden potilaiden näytteissä. Tulos oli samanlainen myös alfa-SMA ja Ki-67 suhteen. Tenaskiiniä voitiin löytää edellisiä niukemmin aggressiivisista näytteistä. Dupuytrenin taudin luonteen lisäselvittelemiseksi geeni- ja proteiinitasolla tehtiin mikroarray, jossa emäsparien pariutumisen avulla selvitetään taudin genomia ja myös sitten tästä aiheutuvien proteiinien ilmentymistä. Kahtatoista Dupuytren potilaan kämmenkalvon kudosnäyttettä verrattiin kolmeen terveeseen verrokki kudosnäytteeseen ja voitiin todeta myoglobiinin ja ROR2:n selkeät pitoisuuden muutokset terveisiin näytteisiin verrattaessa. ROR2 toimii solujen välisten viestien välityksen reseptorina, eli siirtää signaalin solun ulkopuolelta sen sisäpuolelle solun pinnalla olevan kiinnittymiskohdan avulla. Sillä on selkeä merkitys ja tehtävä proliferatiivisissä tapahtumissa, kuten sidekudoksen lisääntymisessä. Mahdollisia kromosomin määrän muutoksia Dupuytren kudoksessa selviteltiin kahdeksantoista kudosnäytteen tutkimisella ja löydösten tulosta verrattiin sitten kahteen normaaliin verrokki kudosnäytteen tulokseen. Tutkimuksessa ei saatu selville kromosomien määrän muutosta, kun muutosten kokonaismäärä on vähäinen tai ainakin alle 50 % kokonaismäärää alhaisempi. Yhteenvetona voidaan todeta, että löytyi histologisia kudoselementtejä, joiden perusteella voidaan ennustaa, onko Dupuyrenin tauti aggressiivisempi ja todennäköisemmin uusiutuva luonteeltaan. ROR2 ei ole aikaisemmin yhdistetty Dupuytrenin kontraktuuraan. Dupuytren kudoksesta ei voitu 44K oligonukleotide mikroarray tekniikalla paljastaa geenimäärien muutoksia
Rysä, J. (Jaana). "Gene expression profiling in experimental models of cardiac load". Doctoral thesis, University of Oulu, 2008. http://urn.fi/urn:isbn:9789514287664.
Texto completoLam, Chi-leung David y 林志良. "Gene expression profiling in non-small cell lung cancer". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B38585777.
Texto completoBergman, Julia. "Aspects of Gene Expression Profiling in Disease and Health". Doctoral thesis, Uppsala universitet, Molekylär och morfologisk patologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-312939.
Texto completoChang, Xiaoqing. "Bayesian Mixtures and Gene Expression Profiling with Missing Data". University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1226090856.
Texto completoNascimento, Helvia. "Caracterização da expressão genica de celulas tumorais de pacientes com adenocarcinoma esporadico do colon". [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310956.
Texto completoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
Made available in DSpace on 2018-08-12T11:53:50Z (GMT). No. of bitstreams: 1 Nascimento_Helvia_D.pdf: 1845231 bytes, checksum: 16f227072d706aacb01f06165bd8a553 (MD5) Previous issue date: 2007
Resumo: Os mecanismos moleculares envolvidos na origem do adenocarcinoma de cólon esporádico (ACE) ainda não estão completamente elucidados. Recentemente, o método da análise seriada da expressão gênica (SAGE) foi descrito como eficaz para identificar a expressão total de genes de tipos celulares diversos, mas esta análise não foi realizada em células epiteliais purificadas do ACE moderadamente diferenciado. Nós caracterizamos pelo método SAGE a expressão gênica total de células epiteliais neoplásicas do cólon de um paciente com ACE moderadamente diferenciado (SAGE CC) e de células epiteliais normais do cólon de um paciente com megacólon chagásico (SAGE CN). Foram geradas, após o seqüenciamento automático, 44.004 e 43.570 tags totais das bibliotecas SAGE CC e SAGE CN, representando 16.484 e 13.479 tags únicas, respectivamente. Na comparação entre as bibliotecas, 171 transcritos diferencialmente expressos foram identificados (P< 0,001; expressão diferencial = 5), incluindo 10% de transcritos que podem representar genes não descritos. As expressões de 10 genes diferencialmente expressos foram quantificadas pela reação em cadeia da polimerase em tempo real (qPCR) na amostra de células epiteliais neoplásicas (SAGE CC), com o intuito de validar os resultados obtidos pelo SAGE, e, posteriormente, em amostras de células epiteliais de outros cinco pacientes com o mesmo tipo de doença. As expressões foram concordantes em 80% dos genes (CEACAM6, KLK6, LYZ, PFN1, S100A8, S100A9, VIL2 e ZFHX1B) e discordantes nos demais 20% (PLA1A e ZNF277). As expressões dos genes de interesse, quantificadas pelos dois métodos, foram similares na amostra SAGE CC e nas amostras dos demais pacientes com a doença. Foram observadas expressões anormais de genes envolvidos com a proliferação e diferenciação celular e com a resposta ao stress em células epiteliais neoplásicas. Foram também visualizadas expressões anormais de genes não relacionados com a doença e de genes ainda não identificados. Em conjunto, os nossos resultados podem contribuir para a identificação de genes relacionados com a origem ou a progressão do ACE moderadamente diferenciado e, ainda, para a descoberta de agentes terapêuticos específicos que controlem a proliferação anormal das células neoplásicas.
Abstract: The molecular mechanisms involved in sporadic colon adenocarcinoma (SCA) are still not completely elucidated. Recently, the serial analysis of gene expression (SAGE) method has allowed the global analysis of genes expressed in diverse cellular types but there are no studies in purified epithelial cells of SCA moderately differenciated. We have characterized through SAGE the global gene expression of neoplastic epithelial cells from a SCA moderately differenciated patient (SAGE CC) and normal epithelial cells from a megacolon patient (SAGE CN). After automatic sequencing, a total of 44.004 tags from SAGE CC and 43.570 tags from SAGE CN profiles were generated, representing 16.484 and 13.479 unique tags, respectively. Comparing both profiles, 171 differentially expressed transcripts were identified (P< 0.001; fold = 5), including 10.0% that may represent novel transcripts. The expression of 10 selected genes was further investigated by realtime polymerase chain reaction (qPCR) in the SCA moderately differenciated epithelial cells sample (SAGE CC), with the purpose of to validate the results obtained by the SAGE method, and also in five epithelial cells samples from the same type of SCA patients. Similar expressions were seen in 80% (CEACAM6, KLK6, LYZ, PFN1, S100A8, S100A9, VIL2 e ZFHX1B) and discordant expressions were seen in 20% (PLA1A e ZNF277) of analysed genes. On SAGE CC sample and samples of the SCA patients, all genes presented similar expressions measured by both methods. We observed abnormal expression of genes involved with cell proliferation and differentiation, and with response to stress in neoplastic epithelial cells. Also, were found abnormal expressions of genes not related with the disease and not identified genes. Together, our results may contribute for the identification of genes involved in the origin or progression of SCA moderately differenciated, as well as for the discovery of new therapeutical agents, with specific action on abnormal proliferation of the neoplastic cells.
Doutorado
Ciencias Basicas
Doutor em Clínica Médica
McKinney, Eoin Fergal. "Gene expression profiling in systemic vasculitis and systemic lupus erythematosus". Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609786.
Texto completoGranberg, Fredrik. "Global Profiling of Host Cell Gene Expression During Adenovirus Infection". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7221.
Texto completoMcKethan, Brandon Lee Dickstein Rebecca. "Gene expression profiling of the nip mutant in Medicago truncatula". [Denton, Tex.] : University of North Texas, 2007. http://digital.library.unt.edu/permalink/meta-dc-3940.
Texto completoBaken, Kirsten Annika. "Immunotoxicogenomics gene expression profiling as a tool to study immunotoxicity /". [Maastricht] : Maastricht : Universitaire Pers Maastricht ; University Library, Universiteit Maastricht [host], 2007. http://arno.unimaas.nl/show.cgi?fid=9329.
Texto completoWill, Malcolm B. "Gene expression profiling of mesenchymal stem cells aged in vitro". Thesis, University of Glasgow, 2010. http://theses.gla.ac.uk/1964/.
Texto completoRomero, Claudia. "CELLULAR IMMUNE RESPONSE AND GENE EXPRESSION PROFILING IN CROHN'S DISE". Doctoral diss., University of Central Florida, 2004. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2704.
Texto completoPh.D.
Other
Burnett College of Biomedical Sciences
Biomolecular Sciences: Ph.D.
Durrant, Wendy E. "Gene expression profiling of the Cf-9 dependent defence response". Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323392.
Texto completoMenon, Suraj. "Integration and biological interpretation of microarry gene expression profiling data". Thesis, Cardiff University, 2009. http://orca.cf.ac.uk/55858/.
Texto completoMcKethan, Brandon Lee. "Gene Expression Profiling of the nip Mutant in Medicago truncatula". Thesis, University of North Texas, 2007. https://digital.library.unt.edu/ark:/67531/metadc3940/.
Texto completoJacobsen, Mette Dorph. "Analysis of Candida albicans gene expression using single cell profiling". Thesis, University of Aberdeen, 2005. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU193586.
Texto completoKan, Takatsugu. "Gene expression profiling in human esophageal cancers using cDNA microarray". Kyoto University, 2003. http://hdl.handle.net/2433/148738.
Texto completoBury, Joanna J. "Gene expression profiling of peripheral tissues in amyotrophic lateral sclerosis". Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/10101/.
Texto completoFoerster, Susann. "Gene expression profiling of human lymph node-positive gastric adenocarcinomas". Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16259.
Texto completoIn this work, gene expression profiles of diffuse and intestinal-type gastric adenocarcinomas were established using the microarray technique. The intestinal type was identified to be a highly proliferative entity with significant overexpression of cell cycle-relevant genes, whereas the diffuse type was proven to be strongly stroma-dependent with significant overexpression of extracellular matrix genes. Thrombospondin 4 (THBS4) was identified as the gene most differentially expressed between the two types with vast mRNA overexpression in diffuse-type tumors. Immunohistochemical studies proved overexpression on protein level and elucidated that THBS4 is a heavily accumulated extracellular constituent of the tumor stroma. Colocalization studies uncovered that THBS4-positive cells are also positive for vimentin and alpha-smooth muscle actin. These data signify that THBS4 is expressed by subpopulations of cancer-associated fibroblasts (CAFs). This was further evidenced by in vitro experiments demonstrating that THBS4 mRNA expression is increased in CAFs of diffuse-type tumors compared to normal gastric fibroblasts. Finally, in vitro coculture studies revealed that transcriptional THBS4 expression in fibroblasts is stimulated by diffuse-type gastric tumor cells. Metastatic involvement of regional lymph nodes (N+) usually accompanies diagnosis of gastric adenocarcinoma and is currently considered the most important parameter for assessment of prognosis. However, estimation of prognosis based on this parameter alone is not sufficiently reliable. In order to identify additional molecular prognosis markers, genes whose expression correlates with clinical outcome of N+ patients were extracted from the microarray data. Via quantitative real-time PCR, several genes, e.g. RAN binding protein 17 and ras-related associated with diabetes, were successfully validated to allow an expression-based stratification of patients with respect to disease-free survival.
Carlén, Lina. "Characterization of psoriasis lesions by protein expression profiling /". Stockholm : Karolinska institutet, 2006. http://diss.kib.ki.se/2006/91-7140-808-8/.
Texto completoBoräng, Stina. "Subtracted Approaches to Gene Expression Analysis in Atherosclerosis". Doctoral thesis, KTH, Biotechnology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3684.
Texto completoGene expression analysis has evolved as an extensive toolfor elucidation of various biological and molecular eventsoccurring in different organisms. A variety of techniques andsoftware tools have been developed to enable easier and morerapid means of exploring the genetic information. A moreeffective approach than exploring the whole content of genesexpressed under certain conditions is to study fingerprintassays or to use subtracted cDNA libraries to identify onlydifferentially expressed genes.
The objective for the work in this thesis has been toexplore differentially expressed genes in atherosclerosis. Thiswas done by applying and modifying a protocol for thesubtractive approach RDA (Representational Difference Analysis)in different model systems.
Initially, the molecular effects of an anti-atheroscleroticdrug candidate were elucidated. In addition, two alternativeapproaches to identify differentially expressed genes obtainedafter iterative rounds of RDA subtraction cycles wereevaluated. This revealed that in most cases, the shotgunapproach in which the obtained gene fragments are clonedwithout any prior selection has clear advantages compared tothe more commonly used selection strategy, whereby distinctbands are excised after gel electrophoresis.
A key process in the atherosclerotic plaque initiation isthe phenotypic change of macrophages into foam cells, which canbe triggered in a model system by using macrophages exposed tooxidised LDL. To investigate the genes expressed in thisprocess, the RDA technique was combined with microarrayanalysis, which allows for selectivity and sensitivity throughRDA, as well as rapid high-throughput analysis usingmicroarrays. The combination of these techniques enablessignificant differences in gene expression to be detected, evenfor weakly expressed genes and the results to be reliablyvalidated in a high throughput manner.
Finally, investigation of the focal nature ofatherosclerotic lesions and gene expression profiling werestudied using in vivo aortic tissues from ApoE-/- and LDLR -/-mice. The study was based on a comparison between localisationsthat are likely, and others that are unlikely, to developatherosclerotic plaques, and the RDA technique was employed toexplore differential gene expression.
Keywords:Representational Difference Analysis,atherosclerosis, gene expression profiling
Bjork, Kathe Elizabeth. "Robust identification of differential gene expression and discrimination /". Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2006.
Buscar texto completoTypescript. Includes bibliographical references (leaves 237-239). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;