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Tesis sobre el tema "Gel filtration chromatography"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 20 de febrero de 2023

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1

Zhang, Qunying. "Characterization of receptors for Escherichia coli 987P using competitive binding assays, thin-layer chromatography and gel filtration chromatography." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0023/MQ51825.pdf.

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2

Ring, Ludwig. "Purification of psychoactive biomolecules in plants using size exclusion chromatography." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-18434.

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<p><em>Size exclusion chromatography</em> (SEC) was applied for purification of psychoactive biomolecules from plants. These molecules are in the same molecular weight range, but do not necessarily share other chemical properties, that makes the SEC technique efficient. By applying SEC as a first purification step much of the co-extractives from the plants can easily be removed. Large amounts of target substance can be obtained with little effort if the system is automated. Combining SEC with a second purification step, consisting of normal phase chromatography, provides high purity of the tar

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3

Gustafsson, Sofia. "Expression and Purification of Murine Tripeptidyl Peptidase II." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-177009.

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Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E. coli and thereafter purify theenzyme from the bacterial lysate. Methods used for t

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4

Suttisansanee, Uthaiwan. "Biochemistry in Bacterioferritin." Thesis, University of Waterloo, 2006. http://hdl.handle.net/10012/2983.

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Bacterioferritin, an iron storage protein having a 24-subunit quaternary structure, was used as a model for the study of host-guest interactions and guest encapsulation, making use of its spherical cage-like structure. A hexahistidine-affinity tag fused to the C-terminus of each bacterioferritin subunit was constructed. The C-terminus of each subunit points toward the inside of the cavity, while the N-terminus is exposed on the surface of the protein. The hexaHistag was able to form strong interactions with a nickel-nitrilotriacetic acid linked dye molecule (guest) and this interactio

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5

Vergnolle, Chantal. "I: purification et caracterisation de proteines de transfert de phospholipides, a partir de feuilles d'epinard (spinacia oleracea l. ). Ii: synthese in vitro des proteines vegetales : methodologie." Paris 6, 1986. http://www.theses.fr/1986PA066254.

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Des proteines, capables de faciliter des mouvements intermembranaires de phospholipides, ont ete isolees a partir de feuilles d'epinard. Ces proteines, appelees proteines de transfert de phospholipides, ont ete purifiees par les techniques classiques de chromatographie (filtration sur gel, echangeurs d'ions) ou par les techniques plus resolutives et plus rapides de chromatographie liquide a haute performance (colonnes echangeuses d'ions ou en phase inverse). Nous avons verifie la purete des fractions par electrophorese en presence de sds

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6

Trevisoli, Edilaine Della Valentina Gonçalves. "Purificação de eliciadores de defesa vegetal em soja e feijoeiro a partir de nematoides fitopatogênicos." Universidade Estadual do Oeste do Paraná, 2016. http://tede.unioeste.br:8080/tede/handle/tede/1475.

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Made available in DSpace on 2017-07-10T17:40:55Z (GMT). No. of bitstreams: 1 Edilaine_D_V_GoncalvesTrevisoli.pdf: 2707364 bytes, checksum: 7b7a664f9727d5909c470f377ee155e8 (MD5) Previous issue date: 2016-02-25<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The induction of resistance in plants to pathogens is an alternative method of disease control, wich involves activation of plant resistance mechanisms such as induction of phytoalexins. The elicitors molecules are able to induce and activate those responses, and therefore, techniques have sought to isolate and charact

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7

Baba, Hamed Mohamed Bey. "Purification et caractérisation de protéases alcalines des larves de Galleria Mellonella." Rouen, 1986. http://www.theses.fr/1986ROUES053.

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Deux protéases alcalines P1 et P2 ont été isolées à partir d'homogénats de larves de Galleria mellonella. Les 2 enzymes ont été séparées par chromatographie sur échangeurs d'ions et purifiées ultérieurement par filtration sur gel. Les 2 protéases se distinguent par leur pH optimum d'action, leur poids moléculaire et leur sensibilité vis-a-vis de différents inhibiteurs de protéases. La répartition anatomique de l'activité protéolytique montre la présence de 2 protéases alcalines similaires à P1 et P2 dans le tube digestif, et d'une protéase similaire à P1 dans le tissu adipeux

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8

Meinerz, Cristiane Claudia. "Indução de mecanismos bioquímicos de defesa em sorgo (Sorghum bicolor) por frações obtidas do decocto de avenca (Adiantum capillus-veneris)." Universidade Estadual do Oeste do Paraná, 2010. http://tede.unioeste.br:8080/tede/handle/tede/1413.

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Made available in DSpace on 2017-07-10T17:37:44Z (GMT). No. of bitstreams: 1 Cristiane_Claudia_Meinerz.pdf: 977726 bytes, checksum: 47602589d307d4398cc6061bcd0d1eeb (MD5) Previous issue date: 2010-02-24<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Induction of resistance involves the activation of plant defense mechanisms in response to treatment with biotic or abiotic elicitors. The application of plant extracts in order to induce resistance mechanisms is an interesting alternative to chemical control, however, besides the presence of inducers, can occur the presence

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9

Kruger, Sarah Jane, and n/a. "Characterisation of Proteins from Grevillea robusta and NMR Studies of the Serine Protease Inhibitor." Griffith University. School of Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040618.150708.

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Proteins that recognise the sugar surface structures on cells have an enormous potential to be used as tools in the characterisation of these structures. A group of proteins, called lectins, have been identified that can bind to carbohydrate complexes on the receptors of cells. The crude extract from Grevillea robusta seeds was found to contain lectin-like proteins that were different from most other lectins, as they would specifically target the receptors of white blood cells and not those found on red blood cells. Therefore, the lectin isolated from G.robusta could be used as a tool to id

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10

Kruger, Sarah Jane. "Characterisation of Proteins from Grevillea robusta and NMR Studies of the Serine Protease Inhibitor." Thesis, Griffith University, 2004. http://hdl.handle.net/10072/366534.

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Proteins that recognise the sugar surface structures on cells have an enormous potential to be used as tools in the characterisation of these structures. A group of proteins, called lectins, have been identified that can bind to carbohydrate complexes on the receptors of cells. The crude extract from Grevillea robusta seeds was found to contain lectin-like proteins that were different from most other lectins, as they would specifically target the receptors of white blood cells and not those found on red blood cells. Therefore, the lectin isolated from G.robusta could be used as a tool to id

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11

Iurkiv, Luciana. "Purificação parcial de compostos biologicamente ativos a partir de Pycnoporus sanguineus para o controle de ferrugem asiática em soja." Universidade Estadual do Oeste do Paraná, 2009. http://tede.unioeste.br:8080/tede/handle/tede/1343.

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Made available in DSpace on 2017-07-10T17:37:27Z (GMT). No. of bitstreams: 1 Luciana Iurkiv.pdf: 711564 bytes, checksum: 5c4d90728ef8f794863f124f3c97e9a7 (MD5) Previous issue date: 2009-02-04<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Induced resistance involves the activation of latent defense mechanisms in plants in response to the treatment with biotic or abiotic agents. The use of crude extracts aimed at inducing resistance mechanisms is an interesting alternative to chemical control, however, in this extracts can occurs besides de presence of inducers, the prese

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12

Rius, Solé M. Àngels. "Estudio de otros compuestos relacionados con la presencia de olor sexual no atribuible al escatol y a la 5@-Androst-16-en-3-ona en grasa dorsal de cerdo." Doctoral thesis, Universitat de Girona, 2000. http://hdl.handle.net/10803/8032.

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En el presente trabajo de tesis se evalúa la posible contribución de otros compuestos al desarrollo del olor sexual mediante la aplicación de técnicas de Head Space dinámico y cromatografía de filtración de gel. El estudio se efectuó en canales de cerdos enteros previamente clasificadas con concentraciónes bajas de escatol (<0,10 µg /g) y androstenona (0,50 µg/ g) , aunque presentaban , según las respuestas de un panel sensorial, olor sexual. La selección de muestras de grasa clasificadas sin olor sexual permitió la comparación estadística de los resultados obtenidos. El análisis de los compue

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13

Bouakaz, Lamine. "Versatile Implementations of an Improved Cell-Free System for Protein Biosynthesis : Functional and structural studies of ribosomal protein L11 and class II release factor RF3. Novel biotechnological approach for continuous protein biosynthesis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6325.

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14

Levert, Luc. "Distribution par filtration sur gel de la matière organique dissoute en fonction du poids moléculaire nominal dans trois types d'eau du Saguenay /." Thèse, Chicoutimi : Université du Québec à Chicoutimi, 1990. http://theses.uqac.ca.

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15

Teychené, Benoît. "Rôle des nanoparticules organiques dans le colmatage membranaire : application au traitement d'eaux usées urbaines par biopéacteur à membranes." Toulouse, INSA, 2008. http://eprint.insa-toulouse.fr/archive/00000256/.

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Le traitement des eaux usées urbaines constitue une phase importante de la gestion globale des ressources en eau. Le bioréacteur à membranes est une option de choix pour répondre à cette demande. Ce procédé de traitement présente plusieurs avantages par rapport aux procédés classiques à boues activées mais reste confronté à l’un des problèmes majeurs de la filtration membranaire : le colmatage de la membrane. De nombreuses études ont mises en évidence l’impact néfaste sur la filtration de composés contenus dans le liquide interstitiel des boues activées définis comme des colloïdes ou des compo

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16

Compin, Sylvie. "Contribution à la caractérisation des brais d'imprégnation : Iinfluence des traitements thermiques." Toulouse, INPT, 1987. http://www.theses.fr/1987INPT010G.

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Etude de la relation entre le pouvoir impregnant des brais et leurs proprietes physico-chimiques. Definition d'un indice de filtrabilite. Caracteristique de l'aptitude des brais a l'impregnation. Etude de l'evolution des proprietes de filtration des brais en fonction des traitements thermiques. Etude des mecanismes responsables de la filtrabilite par fractionnement et caracterisation des fractions du brai

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17

Elisabeth, Patrick. "Purification et caractérisation des enzymes de synthèse des catécholamines, de lipoprotéines et d'acides nucléiques en employant la chromatographie liquide (CLHP) à haute performance par filtration sur gel, d'échange d'ions et d'interaction hydrophobe." Paris 11, 1989. http://www.theses.fr/1989PA112244.

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Notre but a été d'élaborer un protocole expérimental pour la purification rapide des enzymes de synthèse des catécholamines. La chromatographie liquide haute performance (CLHP) par filtration sur gel et échange d'ions a permis de réduire considérablement la durée d'analyse avec un rendement accrue et un gain d 'activité spécifique des enzymes. Les durées d'analyse courtes. Par l'emploi de la CLHP. Ont évité l'hydrolyse des protéines souvent observée en chromatographie classique et due à la présence de certaines protéases. Plusieurs méthodes chromatographiques ont été employées afin d'éliminer

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18

Faye, Audrey Laure. "Mécanismes moléculaires impliqués dans les changements de sensibilité de la Carnitine Palmitoyltransferase 1 hépatique (L-CPT1) de rat vis-à-vis du malonyl-CoA." Paris 7, 2005. http://www.theses.fr/2005PA077018.

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19

Moutete, Hemery Fernand. "Identification et caractérisation des protéines allergéniques de l'huile d'arachide." Nancy 1, 1995. http://www.theses.fr/1995NAN10444.

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20

Jeong, Yong-Dae. "Caractérisation des matières organiques réfractaires dans les effluents traités par voie biologique (contribution de la DCO soluble inerte produite à la DCO soluble résiduelle de boues activées)." Toulouse, INSA, 1995. http://www.theses.fr/1995ISAT0019.

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L'objectif de ce travail est d'expliquer la formation des pms refractaires et de caracteriser ces produits quantitativement et qualitativement lors du traitement d'une eru par boues activees. La premiere partie presente un bilan des connaissances sur des pms a la dco residuelle de boues activees. La deuxieme partie a permis d'evaluer la dco soluble produite dans la dco soluble residuelle de boues activees traitant une eru en mode continu. La troisieme partie presente la cinetique de la formation des pms lors de la degradation du glucose par boues activees en sbr. Dans la quatrieme partie et da

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21

Derappe, Christian. "Étude structurale d'oligosaccharides isolées d'urine humaine de gestation : mise en évidence de l'excrétion de glycosides inconnus phosphoryles ou dérivés du myo-inositol." Paris 6, 1986. http://www.theses.fr/1986PA066325.

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22

Bhoolia, Deena. "Preparation of ribsomal subunits by gel filtration." Thesis, 2016. http://hdl.handle.net/10539/20684.

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A Oiss{!y'tation $uhm'itted in fulfilment of the r~;~airements, for' the degree of f·1aster· oi~'j Science at the University of the ~titwatersrand ' Juhanoasbul"g January 1991<br>An attempt was made to separate ribosomal subunits by gel filtl'ation on Trisacryl GF2000 and Sepharose 4B. Trisacryl GP~2000,a sYllthet'ic gol, , separated rat .ljver rlbosonal subunits orl 'f~'135,em column with a resolution of ""Or3, resulting 'in <'1" 60 rJ impm''ity 'of each of the \~ subunits. subunits" were not resolved. Sepharose 4131 an agarose based gol, separated " the subunits by adsorpttcn chr

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23

鄭宇伸. "Separation of Pure and Immunogenuc Virus-Like Particles Using Gel-Filtration Chromatography Following Immobilized Metal Affinity Chromatography." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/btm5qm.

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碩士<br>國立中興大學<br>生物化學研究所<br>88<br>A purification process was developed to obtain highly pure rVP2H particles, formed by a structural protein (VP2) of infectious bursal disease virus (IBDV) with additional six histidine residues at its C-terminus, for the study of its antigenicity and conformational structure and the development of an efficient subunit vaccine against IBDV infection. The expressed VLPs mimic th authentic structure of the critical epitopes in the native viruses and are capable of inducing a stronger immunological response than does the free form of rVP2H. Those particles in the i

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24

Jack, Alexandria Taylor. "Semi-preparative expression and purification of a recombinant glucocerebrosidase protein with a PTD4 transduction domain: a potential therapeutic strategy for neuronopathic Gaucher’s disease." Thesis, 2012. http://hdl.handle.net/1828/4166.

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Gaucher’s disease (GD) is an autosomal recessive lysosomal storage disorder which is caused by a mutation in the gene encoding acid β-glucocerebrosidase (GBA, EC 3.2.1.45). Deficient activity in GBA leads to a wide variety of clinical phenotypes, including visceral symptoms such as hepatospenomegaly as well as neurological symptoms. Current enzyme replacement therapy is effective in treating visceral symptoms but cannot cross the blood-brain barrier to target neurological manifestations. Another drawback to current therapy is the high cost to patients due to present protein expression strategi

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25

Liu, Kaiyin. "Investigation of the Mechanism and Structure of the Cage-like Complex formed by the Escherichia coli Inducible Lysine Decarboxylase LdcI and the MoxR AAA+ ATPase RavA." Thesis, 2013. http://hdl.handle.net/1807/43098.

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The gram-negative bacteria Escherichia coli, a neutralophile, is remarkable in its defenses against acid stress. Of interest to our laboratory is the inducible lysine decarboxylase (LdcI) system, an acid resistance system which renders acid resistance to E. coli in mild acid stress (~pH 5). It was found that this enzyme forms an extremely large (~3.3 MDa) and tight complex (Kd ~ 0.56 μM) with a MoxR AAA+ ATPase named Regulatory ATPase Variant A (RavA). The cryo-EM structure at 14 Å was determined. Through size-exclusion chromatography (SEC) experiments, the binding sites on both LdcI and RavA

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