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Artículos de revistas sobre el tema "Escherichia Identification"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 5 de febrero de 2022

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1

Parin, U., S. Kirkan, SS Arslan y HT Yuksel. "Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea". Veterinární Medicína 63, No. 3 (28 de marzo de 2018): 110–16. http://dx.doi.org/10.17221/156/2017-vetmed.

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The aim of this study was to determine the incidence of Escherichia fergusonii in dairy cattle with clinical signs of diarrhoea. The specimens were obtained from three different farms in Denizli province of Turkey, between August 2016 and December 2016. Rectal contents of 57 Holstein-friesian dairy cattle with diarrhoea were collected from farms located in the Aegean Region (Denizli province, Turkey). Rectal swabs were inoculated into enrichment, differential and selective culture media. A total of 49 (86%) Escherichia spp. were isolated by phenotypic identification from 57 rectal swab samples. Presumptive E. fergusonii isolates were tested with the API 20E identification kit and all isolates (100%) were identified as E. coli. Primers targeting specific E. coli and E. fergusonii and genes, including the beta-glucuronidase enzyme, conserved hypothetical cellulose synthase protein and regulator of cellulose synthase and hypothetical protein, putative transcriptional activator for multiple antibiotic resistance were used for detection and differentiation of E. coli and E. fergusonii. Thirteen of the 49 E. coli-verified isolates were identified as E. fergusonii after duplex PCR using EFER 13- and EFER YP-specific primers. Confirmation of strain identity was conducted using Sanger sequencing analysis. The rates of antibiotic resistance of E. fergusonii to penicillin G and erythromycin were 100% and 77%, respectively. In conclusion, field strains of E. fergusonii were detected in cattle with diarrhoea in Turkey, and the strains were found to be resistant to multiple antibiotics.

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2

Hachim Mohammed, Husham, Mohammed Flayyih Tareef y Ali Kamal Mohammed. "Molecular Identification of Isolate from Escherichia coli Isolates from Dialysis Patients". Journal of Pure and Applied Microbiology 12, n.º 4 (30 de diciembre de 2018): 2087–94. http://dx.doi.org/10.22207/jpam.12.4.45.

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3

Freestone, P., S. Grant, I. Toth y V. Norris. "Identification of phosphoproteins in Escherichia coli". Molecular Microbiology 15, n.º 3 (febrero de 1995): 573–80. http://dx.doi.org/10.1111/j.1365-2958.1995.tb02270.x.

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4

Lindsey, Rebecca L., L. Garcia-Toledo, D. Fasulo, L. M. Gladney y N. Strockbine. "Multiplex polymerase chain reaction for identification of Escherichia coli , Escherichia albertii and Escherichia fergusonii". Journal of Microbiological Methods 140 (septiembre de 2017): 1–4. http://dx.doi.org/10.1016/j.mimet.2017.06.005.

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5

York, Mary K., Ellen Jo Baron, Jill E. Clarridge, Richard B. Thomson y Melvin P. Weinstein. "Multilaboratory Validation of Rapid Spot Tests for Identification of Escherichia coli". Journal of Clinical Microbiology 38, n.º 9 (2000): 3394–98. http://dx.doi.org/10.1128/jcm.38.9.3394-3398.2000.

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To validate the accuracy of rapid tests for identification ofEscherichia coli, five laboratories sequentially collected 1,064 fresh, clinically significant strains with core criteria of indole-positive, oxidase-negative, nonspreading organisms on sheep blood agar plates (BAP), having typical gram-negative rod plate morphology, defined as good growth on gram-negative rod-selective media. An algorithm using beta-hemolysis on BAP, lactose reaction on eosin-methylene blue or MacConkey agar,l-pyrrolidonyl-β-naphthylamide (PYR), and 4-methylumbelliferyl-β-d-glucuronide (MUG) was evaluated. Identifications using the algorithm were compared to those obtained using commercial kit system identifications. One thousand strains wereE. coli and 64 were not E. coli by kit identifications, which were supplemented with conventional biochemical testing of low probability profiles. Of the 1,064 isolates meeting the core criteria, 294 were beta-hemolytic and did not require further testing to be identified as E. coli. None of the 64 non-E. coli strains were hemolytic, although other indole-positive, lactose-negative species were found to be hemolytic when further strains were examined in a follow-up study. Of the remaining strains, 628 were identified as E. coli by a lactose-positive and PYR-negative reaction. For nonhemolytic, lactose-negative E. coli, PYR was not helpful, but a positive MUG reaction identified 65 of 78 isolates as E. coli. The remaining 13 E. coli strains required kit identifications. This scheme for E. coli identification misidentified three non-E. coli strains as E. coli, for an error rate of 0.3%. A total of 13 kit identifications, 657 PYR tests, and 113 MUG tests were needed to identify 1,000 E. coli strains with the algorithm. The use of this rapid system saves laboratory resources, provides timely identifications, and yields rare misidentifications.

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6

Lee, Yun-Song y Myung-Hee Chung. "Identification of Escherichia coli 8-oxoguanine endonuclease". Experimental & Molecular Medicine 32, n.º 3 (septiembre de 2000): 155–60. http://dx.doi.org/10.1038/emm.2000.26.

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7

Ero, R., L. Peil, A. Liiv y J. Remme. "Identification of pseudouridine methyltransferase in Escherichia coli". RNA 14, n.º 10 (28 de agosto de 2008): 2223–33. http://dx.doi.org/10.1261/rna.1186608.

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8

Rotar, Ancuta Mihaela, Cristina Anamaria Semeniuc, Sorin Apostu, Carmen Pop, Mihaela Duma, Ramona Suharoschi y Larisa Giura. "Identification and Prevalence of Escherichia coli and Escherichia coli O157: H7 in Foods". Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology 70, n.º 2 (13 de noviembre de 2013): 139. http://dx.doi.org/10.15835/buasvmcn-fst:9392.

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The objective of this study is to investigate the incidence of Escherichia coli in animal and non-animal foods, and mainly the incidence of the serotype O157: H7 producing verotoxin. The presence of common Escherichia coli and Escherichia coli O157: H7 in various foods (of animal and non animal origin) was performed in Transylvania area. We analyzed a total of one hundred forty-one samples of minced meat, one hundred twenty-six samples of meat , twenty six samples of meat products, five samples of alcoholic beverages, three samples of seafood, one hundred samples of cheese from pasteurized milk, seventeen samples of butter, four samples of vegetables and one sample of milk powder, using the standard cultural method and Vidas Eco method for E. coli O157: H7 strains. E. coli was identified in 50 samples of minced meat, 55 samples of meat prepared, 4 samples of meat products, 2 samples of alcoholic beverages, 25 samples of cheese from pasteurized milk, 6 samples of butter and 1 sample of vegetables. In this study were not been identified any foods contaminated with the E. coli O157: H7 serotype. The results of this reasearch have demostrated that E. coli wich represents a hygienic indicator of recent food contamination, can be destroyed with heat treatment and hygienic handling of foods. Our country over the years has been among the few countries where the incidence of the E. coli O157: H7 serotype has been minimal.

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9

Blum, S., E. D. Heller, O. Krifucks, S. Sela, O. Hammer-Muntz y G. Leitner. "Identification of a bovine mastitis Escherichia coli subset". Veterinary Microbiology 132, n.º 1-2 (noviembre de 2008): 135–48. http://dx.doi.org/10.1016/j.vetmic.2008.05.012.

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10

N, Padmaja, Anand Acharya y Nageswar Rao P. "IDENTIFICATION OF UROVIRULENT MARKERS IN UROPATHOGENIC ESCHERICHIA COLI". Journal of Evolution of Medical and Dental Sciences 1, n.º 4 (30 de octubre de 2012): 578–81. http://dx.doi.org/10.14260/jemds/90.

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11

Gomes, T. A., M. R. Toledo, L. R. Trabulsi, P. K. Wood y J. G. Morris. "DNA probes for identification of enteroinvasive Escherichia coli." Journal of Clinical Microbiology 25, n.º 10 (1987): 2025–27. http://dx.doi.org/10.1128/jcm.25.10.2025-2027.1987.

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12

Brandsma, J. A., M. de Ruijter, J. Brouwer y P. van de Putte. "Identification of the uvrA6 mutation of Escherichia coli." Journal of Bacteriology 170, n.º 2 (1988): 1012–14. http://dx.doi.org/10.1128/jb.170.2.1012-1014.1988.

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13

Pass, M. A., R. Odedra y R. M. Batt. "Multiplex PCRs for Identification of Escherichia coliVirulence Genes". Journal of Clinical Microbiology 38, n.º 5 (2000): 2001–4. http://dx.doi.org/10.1128/jcm.38.5.2001-2004.2000.

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PCRs were developed to detect 11 Escherichia colivirulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

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14

Van Bost, S., E. Jacquemin, E. Oswald y J. Mainil. "Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli". Journal of Clinical Microbiology 41, n.º 9 (1 de septiembre de 2003): 4480–82. http://dx.doi.org/10.1128/jcm.41.9.4480-4482.2003.

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15

Queiroz, D. M. M., E. N. Mendes, M. R. F. Toledo y L. R. Trabulsi. "Identification of a new enteroinvasive Escherichia coli strain". Research in Microbiology 141, n.º 6 (enero de 1990): 703–6. http://dx.doi.org/10.1016/0923-2508(90)90064-w.

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16

Varga, Frank J. y Joseph R. Dlpersio. "Use of the RIM ® Escherichia coli Kit for Rapid Identification of Escherichia coli". American Journal of Clinical Pathology 86, n.º 6 (1 de diciembre de 1986): 761–64. http://dx.doi.org/10.1093/ajcp/86.6.761.

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17

Choi, Yong-Jun, Keun-Wook Lee, Hyung-Joo Kwon y Doo-Sik Kim. "Identification of Immunostimulatory Oligodeoxynucleotide from Escherichia coli Genomic DNA". BMB Reports 39, n.º 6 (30 de noviembre de 2006): 788–93. http://dx.doi.org/10.5483/bmbrep.2006.39.6.788.

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18

Machado, J., F. Grimont y P. A. D. Grimont. "Computer identification of Escherichia coli rRNA gene restriction patterns". Research in Microbiology 149, n.º 2 (febrero de 1998): 119–35. http://dx.doi.org/10.1016/s0923-2508(98)80027-2.

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19

Kiel, M. "Identification of a ribosomal ATPase in Escherichia coli cells". Biochimie 81, n.º 12 (diciembre de 1999): 1097–108. http://dx.doi.org/10.1016/s0300-9084(99)00352-1.

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20

DiGate, R. J. y K. J. Marians. "Identification of a potent decatenating enzyme from Escherichia coli." Journal of Biological Chemistry 263, n.º 26 (septiembre de 1988): 13366–73. http://dx.doi.org/10.1016/s0021-9258(18)37713-5.

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21

Germani, Y. "IDENTIFICATION AND ASSAY METHODS FOR ESCHERICHIA COLIENTEROTOXINS, (IN ENGLISH)". Pediatric Infectious Disease Journal 7, n.º 5 (mayo de 1988): 373. http://dx.doi.org/10.1097/00006454-198805000-00034.

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22

Lindenthal, Christoph y Eric A. Elsinghorst. "Identification of a Glycoprotein Produced by Enterotoxigenic Escherichia coli". Infection and Immunity 67, n.º 8 (1 de agosto de 1999): 4084–91. http://dx.doi.org/10.1128/iai.67.8.4084-4091.1999.

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ABSTRACT Enterotoxigenic Escherichia coli (ETEC) strain H10407 is capable of invading epithelial cell lines derived from the human ileocecum and colon in vitro. Two separate chromosomally encoded invasion loci (tia and tib) have been cloned from this strain. These loci direct nonadherent and noninvasive laboratory strains of E. coli to adhere to and invade cultured human intestinal epithelial cells. The tib locus directs the synthesis of TibA, a 104-kDa outer membrane protein that is directly correlated with the adherence and invasion phenotypes. TibA is synthesized as a 100-kDa precursor (preTibA) that must be modified for biological activity. Outer membranes of recombinant E. coliexpressing TibA or preTibA were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted to nitrocellulose. The presence of glycoproteins was detected by oxidization of carbohydrates with periodate and labeling with hydrazide-conjugated digoxigenin. Only TibA could be detected as a glycoprotein. Complementation experiments with tib deletion mutants of ETEC strain H10407 demonstrate that the TibA glycoprotein is expressed in H10407, that the entire tib locus is required for TibA synthesis, and that TibA is the only glycoprotein produced by H10407. Protease treatment of intact H10407 cells removes the carbohydrates on TibA, suggesting that they are surface exposed. TibA shows homology with AIDA-I from diffuse-adhering E. coliand with pertactin precursor from Bordetella pertussis. Both pertactin and AIDA-I are members of the autotransporter family of outer membrane proteins and are afimbrial adhesins that play an important role in the virulence of these organisms. Analysis of the predicted TibA amino acid sequence indicates that TibA is also an autotransporter. Analysis of the tib locus DNA sequence revealed an open reading frame with similarity to RfaQ, a glycosyltransferase. The product of this tib locus open reading frame is proposed to be responsible for TibA modification. These results suggest that TibA glycoprotein acts as an adhesin that may participate in the disease process.

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23

ZHU, G., J. WANG y X. ZHU. "Identification of 987P Protein Receptors for Enterotoxigenic Escherichia coli". Chinese Journal of Biotechnology 24, n.º 3 (marzo de 2008): 363–67. http://dx.doi.org/10.1016/s1872-2075(08)60017-5.

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24

Mehl, Ryan A., Cynthia Kinsland y Tadhg P. Begley. "Identification of the Escherichia coliNicotinic Acid Mononucleotide Adenylyltransferase Gene". Journal of Bacteriology 182, n.º 15 (1 de agosto de 2000): 4372–74. http://dx.doi.org/10.1128/jb.182.15.4372-4374.2000.

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ABSTRACT The gene (ybeN) coding for nicotinate mononucleotide adenylyltransferase, an NAD(P) biosynthetic enzyme, has been identified and overexpressed in Escherichia coli. This enzyme catalyzes the reversible adenylation of nicotinate mononucleotide and shows product inhibition. The rate of adenylation of nicotinate mononucleotide is at least 20 times faster than the rate of adenylation of nicotinamide mononucleotide.

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25

Maynes, Jason T., Richard G. Yuan y Floyd F. Snyder. "Identification, Expression, and Characterization of Escherichia coli Guanine Deaminase". Journal of Bacteriology 182, n.º 16 (15 de agosto de 2000): 4658–60. http://dx.doi.org/10.1128/jb.182.16.4658-4660.2000.

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ABSTRACT Using the human cDNA sequence corresponding to guanine deaminase, the Escherichia coli genome was scanned using the Basic Local Alignment Search Tool (BLAST), and a corresponding 439-residue open reading frame of unknown function was identified as having 36% identity to the human protein. The putative gene was amplified, subcloned into the pMAL-c2 vector, expressed, purified, and characterized enzymatically. The 50.2-kDa protein catalyzed the conversion of guanine to xanthine, having a Km of 15 μM with guanine and a k cat of 3.2 s−1. The bacterial enzyme shares a nine-residue heavy metal binding site with human guanine deaminase, PG[FL]VDTHIH, and was found to contain approximately 1 mol of zinc per mol of subunit of protein. The E. coli guanine deaminase locus is 3′ from an open reading frame which shows homology to a bacterial purine base permease.

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26

Le Brun, N. E., S. C. Andrews, J. R. Guest, P. M. Harrison, G. R. Moore y A. J. Thomson. "Identification of the ferroxidase centre of Escherichia coli bacterioferritin". Biochemical Journal 312, n.º 2 (1 de diciembre de 1995): 385–92. http://dx.doi.org/10.1042/bj3120385.

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The bacterioferritin (BFR) of Escherichia coli takes up iron in the ferrous form and stores it within its central cavity as a hydrated ferric oxide mineral. The mechanism by which oxidation of iron (II) occurs in BFR is largely unknown, but previous studies indicated that there is ferroxidase activity associated with a site capable of forming a dinuclear-iron centre within each subunit [Le Brun, Wilson, Andrews, Harrison, Guest, Thomson and Moore (1993) FEBS Lett. 333, 197-202]. We now report site-directed mutagenesis experiments based on a putative dinuclear-metal-ion-binding site located within the BFR subunit. The data reveal that this dinuclear-iron centre is located at a site within the four-alpha-helical bundle of each subunit of BFR, thus identified as the ferroxidase centre of BFR. The metal-bound form of the centre bears a remarkable similarity to the dinuclear-iron sites of the hydroxylase subunit of methane mono-oxygenase and the R2 subunit of ribonucleotide reductase. Details of how the dinuclear centre of BFR is involved in the oxidation mechanism were investigated by studying the inhibition of iron (II) oxidation by zinc (II) ions. Data indicate that zinc (II) ions bind at the ferroxidase centre of apo-BFR in preference to iron (II), resulting in a dramatic reduction in the rate of oxidation. The mechanism of iron (II) oxidation is discussed in the light of this and previous work.

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27

Awano, Naoki, Masaru Wada, Hirotada Mori, Shigeru Nakamori y Hiroshi Takagi. "Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases". Applied and Environmental Microbiology 71, n.º 7 (julio de 2005): 4149–52. http://dx.doi.org/10.1128/aem.71.7.4149-4152.2005.

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ABSTRACT In Escherichia coli, three additional proteins having l-cysteine desulfhydrase activity were identified as O-acetylserine sulfhydrylase-A, O-acetylserine sulfhydrylase-B, and MalY protein, in addition to tryptophanase and cystathionine β-lyase, which have been reported previously. The gene disruption for each protein was significantly effective for overproduction of l-cysteine and l-cystine. Growth phenotype and transcriptional analyses suggest that tryptophanase contributes primarily to l-cysteine degradation.

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28

Chart, H., T. Cheasty y B. Rowe. "Serological identification of infection by verocytotoxin-producing Escherichia coli". Letters in Applied Microbiology 23, n.º 5 (noviembre de 1996): 322–24. http://dx.doi.org/10.1111/j.1472-765x.1996.tb00199.x.

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29

van den Bosch, J. F., J. H. Hendriks, I. Gladigau, H. M. Willems, P. K. Storm y F. K. de Graaf. "Identification of F11 fimbriae on chicken Escherichia coli strains." Infection and Immunity 61, n.º 3 (1993): 800–806. http://dx.doi.org/10.1128/iai.61.3.800-806.1993.

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30

Kang, Sung-Min, Ji-Woong Choi, Youngkyun Lee, Su-Hyung Hong y Heon-Jin Lee. "Identification of microRNA-Size, Small RNAs in Escherichia coli". Current Microbiology 67, n.º 5 (20 de junio de 2013): 609–13. http://dx.doi.org/10.1007/s00284-013-0411-9.

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31

Liu, H., H. Wang, Z. Shi, Q. Liu, J. Zhu, N. He, H. Wang y Z. Lu. "Identification of Escherichia coli O157:H7 with Oligonucleotide Arrays". Bulletin of Environmental Contamination and Toxicology 71, n.º 4 (1 de octubre de 2003): 826–32. http://dx.doi.org/10.1007/s00128-003-0209-8.

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32

Pestariati, Dr y Dr Suhariyadi. "Detection of CTX-M Gene in Escherichia coli Producing Extended Spectrum Beta Lactamase (ESBL) Isolated from Patients with Urinary Tract Infection". International Journal of Medical Science and Clinical Invention 8, n.º 09 (7 de septiembre de 2021): 5610–14. http://dx.doi.org/10.18535/ijmsci/v8i09.05.

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Escherichia coli is a Gram-negative bacterium from Enterobacteriaceae family causes urinary tract infections (UTI). A major problem encountered in antibiotics therapy is Multiple Drug Resistant Organisms (MDROs). MDROs occur because of the presence of resistance coding genes such as CTX-M which causes bacteria to produce the Extended Spectrum Beta-Lactamase (ESBL) enzyme. This study aims to detect the presence of the CTX-M gene in Extended-Spectrum Beta Lactamase (ESBL) Escherichia coli isolated from UTI patients. The study was conducted at the Institute of Tropical Disease (ITD) Airlangga University, Jl. Mulyorejo Campus C Surabaya, Indonesia for polymerase chain reaction (PCR) examination. The isolation and identification of Escherichia coli bacteria were carried out at the Microbiology Laboratory Department in Poltekkes Kemenkes Surabaya, Jl. Karangmenjangan 18A, Surabaya, Indonesia. Conventional identification of Escherichia coli and the presence of the CTX-M gene were observed using the PCR method. The results showed that 18 of 30 samples (60%) were caused by Escherichia coli. Escherichia coli producing ESBL was found in 15 samples (83%), of which 12 samples (80%) showed the presence of the CTX-M gene. Keywords : CTX-M gene, Extended-Spectrum Beta Lactamase (ESBL), Escherichia coli

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33

Yaguchi, Kazuya, Takashi Mikami, Kazuki Igari, Yusuke Yoshida, Katsushi Yokoyama y Kozo Makino. "Identification of LexA regulated promoters in Escherichia coli O157:H7". Journal of General and Applied Microbiology 57, n.º 4 (2011): 219–30. http://dx.doi.org/10.2323/jgam.57.219.

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34

Fujikawa, Norie, Hitoshi Kurumizaka, Mitsuyoshi Yamazoe, Sota Hiraga y Shigeyuki Yokoyama. "Identification of functional domains of the Escherichia coli SeqA protein". Biochemical and Biophysical Research Communications 300, n.º 3 (enero de 2003): 699–705. http://dx.doi.org/10.1016/s0006-291x(02)02891-7.

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35

Puño-Sarmiento, Juan, Luis Gazal, Leonardo Medeiros, Erick Nishio, Renata Kobayashi y Gerson Nakazato. "Identification of Diarrheagenic Escherichia coli Strains from Avian Organic Fertilizers". International Journal of Environmental Research and Public Health 11, n.º 9 (28 de agosto de 2014): 8924–39. http://dx.doi.org/10.3390/ijerph110908924.

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36

Wang, Baoyu, Susan P. Jordan y Marilyn Schuman Jorns. "Identification of a pterin derivative in Escherichia coli DNA photolyase". Biochemistry 27, n.º 12 (junio de 1988): 4222–26. http://dx.doi.org/10.1021/bi00412a003.

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37

Walters, Matthew S. y Harry L. T. Mobley. "Identification of uropathogenic Escherichia coli surface proteins by shotgun proteomics". Journal of Microbiological Methods 78, n.º 2 (agosto de 2009): 131–35. http://dx.doi.org/10.1016/j.mimet.2009.04.013.

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38

Tree, Jai J., Sander Granneman, Sean P. McAteer, David Tollervey y David L. Gally. "Identification of Bacteriophage-Encoded Anti-sRNAs in Pathogenic Escherichia coli". Molecular Cell 55, n.º 2 (julio de 2014): 199–213. http://dx.doi.org/10.1016/j.molcel.2014.05.006.

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39

Carson, C. Andrew, Brian L. Shear, Mark R. Ellersieck y Amha Asfaw. "Identification of Fecal Escherichia colifrom Humans and Animals by Ribotyping". Applied and Environmental Microbiology 67, n.º 4 (1 de abril de 2001): 1503–7. http://dx.doi.org/10.1128/aem.67.4.1503-1507.2001.

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ABSTRACT Fecal pollution of water resources is an environmental problem of increasing importance. Identification of individual host sources of fecal Escherichia coli, such as humans, pets, production animals, and wild animals, is prerequisite to formulation of remediation plans. Ribotyping has been used to distinguish fecalE. coli of human origin from pooled fecal E. coli isolates of nonhuman origin. We have extended application of this technique to distinguishing fecal E. coli ribotype patterns from human and seven individual nonhuman hosts. Classification accuracy was best when the analysis was limited to three host sources. Application of this technique to identification of host sources of fecal coliforms in water could assist in formulation of pollution reduction plans.

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40

Blum, S., S. Sela, O. Hammer-Muntz, O. Krifucks, L. Weisbelith, D. Heller y G. Leitner. "Identification of adaptive traits of bovine mastitis Escherichia coli strains". Veterinary Immunology and Immunopathology 128, n.º 1-3 (marzo de 2009): 262. http://dx.doi.org/10.1016/j.vetimm.2008.10.111.

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41

Kuhnert, P., J. Nicolet y J. Frey. "Rapid and accurate identification of Escherichia coli K-12 strains." Applied and environmental microbiology 61, n.º 11 (1995): 4135–39. http://dx.doi.org/10.1128/aem.61.11.4135-4139.1995.

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42

Zhou, Z. y M. Syvanen. "Identification and sequence of the drpA gene from Escherichia coli." Journal of Bacteriology 172, n.º 1 (1990): 281–86. http://dx.doi.org/10.1128/jb.172.1.281-286.1990.

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43

TSUKAMOTO, Teizo y Takao KAWAI. "Identification of Escherichia coli O157 Antigen by Polymerase Chain Reaction". Journal of the Japanese Association for Infectious Diseases 72, n.º 7 (1998): 738–41. http://dx.doi.org/10.11150/kansenshogakuzasshi1970.72.738.

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44

Barrett, Claire M. L., Joanne E. Mathers y Colin Robinson. "Identification of key regions within the Escherichia coli TatAB subunits". FEBS Letters 537, n.º 1-3 (29 de enero de 2003): 42–46. http://dx.doi.org/10.1016/s0014-5793(03)00068-1.

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45

Shulman, L. P. "Identification of Escherichia coli Genes Associated with Urinary Tract Infections". Yearbook of Obstetrics, Gynecology and Women's Health 2012 (enero de 2012): 295. http://dx.doi.org/10.1016/j.yobg.2012.06.015.

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46

Cook, William R. y Lawrence I. Rothfield. "Nucleoid-Independent Identification of Cell Division Sites in Escherichia coli". Journal of Bacteriology 181, n.º 6 (15 de marzo de 1999): 1900–1905. http://dx.doi.org/10.1128/jb.181.6.1900-1905.1999.

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Resumen

ABSTRACT The mechanism used by Escherichia coli to determine the correct site for cell division is unknown. In this report, we have attempted to distinguish between a model in which septal position is determined by the position of the nucleoids and a model in which septal position is predetermined by a mechanism that does not involve nucleoid position. To do this, filaments with extended nucleoid-free regions adjacent to the cell poles were produced by simultaneous inactivation of cell division and DNA replication. The positions of septa that formed within the nucleoid-free zones after division was allowed to resume were then analyzed. The results showed that septa were formed at a uniform distance from cell poles when division was restored, with no relation to the distance from the nearest nucleoid. In some cells, septa were formed directly over nucleoids. These results are inconsistent with models that invoke nucleoid positioning as the mechanism for determining the site of division site formation.

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47

Kalapos, M. P., G. J. Cao, S. R. Kushner y N. Sarkar. "Identification of a Second Poly(A) Polymerase in Escherichia coli". Biochemical and Biophysical Research Communications 198, n.º 2 (enero de 1994): 459–65. http://dx.doi.org/10.1006/bbrc.1994.1067.

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48

Singer, J. T., C. S. Barbier y S. A. Short. "Identification of the Escherichia coli deoR and cytR gene products." Journal of Bacteriology 163, n.º 3 (1985): 1095–100. http://dx.doi.org/10.1128/jb.163.3.1095-1100.1985.

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49

Mao, B. H., Y. F. Chang, J. Scaria, C. C. Chang, L. W. Chou, N. Tien, J. J. Wu et al. "Identification of Escherichia coli Genes Associated with Urinary Tract Infections". Journal of Clinical Microbiology 50, n.º 2 (9 de noviembre de 2011): 449–56. http://dx.doi.org/10.1128/jcm.00640-11.

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50

Toma, C., Y. Lu, N. Higa, N. Nakasone, I. Chinen, A. Baschkier, M. Rivas y M. Iwanaga. "Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli". Journal of Clinical Microbiology 41, n.º 6 (1 de junio de 2003): 2669–71. http://dx.doi.org/10.1128/jcm.41.6.2669-2671.2003.

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