Tesis: "Effect of temperature on food crops"

1

Phillips, Lisa Elaine. "The effect of low temperature on Salmonella". Thesis, University of Exeter, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286534.

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Walden, Ronald Francis. "Influences of supraoptimal root-zone temperature on the medium solution and growth of woody nursery crops". Diss., Virginia Tech, 1993. http://hdl.handle.net/10919/39733.

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3

James, Neil Anthony. "Evaluating the effect of temperature on the planktonic food-web". Thesis, University of Liverpool, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511064.

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4

Patana-anake, Pakanat. "The Effect of Temperature, pH, and Food additives on Tomato Product Volatile Levels". The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397135614.

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5

Tyrer, Helen Linsey. "The effect of storage temperature on the measured predicted shelf life of chilled prepared foods". Thesis, Manchester Metropolitan University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266441.

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6

Bozkurt, Murat. "Effect Of Different Wheat Varieties On Pasta Quality". Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614874/index.pdf.

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There are about 40 durum wheat varieties registered in Turkey. Around 10 varieties are being commonly cultivated. Amanos 97, Ç
esit 1252, Ege 88, Firat 93, Fuatbey 2000, Burgos, Kiziltan 91, Sariç
anak 98, Svevo and Zenith durum wheat varieties were used in this study. Semolina and pasta have been produced in an industrial plant under constant process conditions from these varieties. In general, pasta quality is determined by three main factors, the raw materials, the production recipe and the production process. In this study, the production recipe and the production process were fixed and only durum wheat varieties were variable. So, the effects coming from the production recipe and the production process on pasta quality were eliminated. Physical, chemical and sensory properties of these pasta were determined and the effect of different durum wheat varieties on pasta quality has been investigated to give a clear idea to the Turkish pasta industry about the most commonly grown durum wheat varieties in Turkey, hoping that Turkish pasta in domestic and international Markets will continue to grow with the help of clear understanding about the quality characteristics of the durum wheat they are using. v The important criteria of acceptability of pasta products is its cooking quality and cooking quality of pasta products is of importance to consumers and also to wheat producers, breeders and manufacturers. The pasta samples produced from Svevo, Zenith and Firat 93 varieties got the higher scores than others in pasta cooking quality while those produced from Sariç
anak 98, Ç
esit 1252, Kiziltan 91, and Fuatbey 2000 durum wheat were evaluated as relatively low. The main reasons of cooking quality differences can be interpreted as a result of the difference in protein quantity and quality of the varieties. The bright yellow color of pasta products, rather than cooking behavior taste, is reported to be one of the most important considerations in assessing durum wheat quality. In this study, the pasta samples produced from Svevo, Zenith and Burgos varieties got the higher scores than others in pasta color (yellowness) while that produced from Firat 93 had the lowest yellow color. The main reasons of yellow color differences can be interpreted as a result of the differences in carotenoid pigments, lipoxygenase and peroxidase enzymes contents of the varieties.

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7

Kong, Emily S. "EFFECT OF pH AND TEMPERATURE ON THE BINDING INTERACTIONS OF CAFFEINE AND CHLOROGENIC ACID WITH SODIUM CASEINATE". DigitalCommons@CalPoly, 2013. https://digitalcommons.calpoly.edu/theses/947.

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Coffee is a popular and well-loved beverage consumed worldwide by millions of people every day. While most patrons of coffee do so because of its unique and satisfying taste, consumers may be unaware of the potential beneficial health effects it also imparts. The antioxidants found in green coffee beans collectively known as chlorogenic acids (CGA) and caffeine are two of the most abundant bioactive compounds present in coffee. Both these bioactive compounds have been implicated in many studies to impart a wide range of health benefits, from reducing the risk of Type 2 diabetes, to their use as aides in weight management. Indeed, epidemiological studies on people who consume moderate amounts of coffee on a regular basis have unanimously shown benefits to overall health. While caffeine and CGA are naturally occurring compounds in coffee, their potential in conferring beneficial health effects warrant research into other potential food matrixes that can be used to bind and deliver these bioactive compounds into foods that do not naturally contain them. Milk proteins, specifically caseins, have been shown to be excellent vehicles to both bind and deliver sensitive bioactive compounds of various chemical and physical properties. Caseins have been shown in numerous studies to successfully bind to molecules such as vitamin D2, ω-3 polyunsaturated fatty acids (DHA), and iron to name a few. Because caseins exhibit high versatility in binding a variety of molecules, caseins were the milk protein of choice for the experiments in this thesis. Polyphenols have been the subject of many studies on its binding capacity with milk proteins, but research on the binding capacity of caffeine with caseins is limited. Therefore, the objectives of this thesis are threefold: 1) develop, optimize and validate an HPLC method for the accurate and simultaneous determination of caffeine and CGA, 2) establish a procedure by which caffeine and CGA bind to sodium caseinate, and 3) determine the optimal treatment conditions of pH and temperature to increase binding interactions and speculate on the mechanism of binding for each bioactive compound. A reversed phase HPLC (RP-HPLC) method was developed and subjected to validation studies with good results in linearity (caffeine R2 = 0.9992, CGA R2 = 0.9995) and precision (RSD of caffeine <1%, RSD of CGA <2%). The developed method also demonstrated selectivity for caffeine and CGA. This method was then used to analyze sodium caseinate samples containing caffeine and 5-CGA. The results from these studies have shown that binding interactions between caffeine and sodium caseinate are temperature dependent (p < 0.01) whereas binding interactions between CGA and sodium caseinate are influenced by both pH and temperature (p < 0.01). Elucidating the binding mechanisms of caffeine and CGA to sodium caseinate and providing a sensitive analytical technique by which these compounds can be accurately quantified may facilitate future research involving the use of caffeine and CGA in many other facets, as well as promoting its increased use in the dairy industry.

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8

Han, Yafei. "Effect of fruit types and temperature on formation of volatiles in the lipoxygenase (LOX) pathway". The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1430955718.

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9

Kabbara, Salam. "EFFECT OF COOKING TIME AND TEMPERATURE ON HARDNESS AND ANTI-NUTRITIONAL FACTORS OF TEPARY BEAN". Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275284.

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10

Sandhi, Arifin. "ARSENIC REMOVAL BY PHYTOFILTRATION AND SILICON TREATMENT : A POTENTIAL SOLUTION FOR LOWERING ARSENIC CONCENTRATIONS IN FOOD CROPS". Doctoral thesis, KTH, Mark- och vattenteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-203995.

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Use of arsenic-rich groundwater for crop irrigation can increase the arsenic (As) content in food crops and act as a carcinogen, compromising human health. Using aquatic plant based phytofiltration is a potential eco-technique for removing arsenic from water. The aquatic moss species Warnstorfia fluitans grows naturally in mining areas in northern Sweden, where high concentrations of arsenic occur in lakes and rivers. This species was selected as a model for field, climate chamber and greenhouse studies on factors governing arsenic removal and arsenic phytofiltration of irrigation water. The arsenic and silicon (Si) concentrations in soil, water and plant samples were measured by AAS (atomic absorption spectrophotometry), while arsenite and arsenate species were determined using AAS combined with high pressure liquid chromatography (HPLC) with an anion exchange column. The arsenic content in grains of hybrid and local aromatic rice (Oryza sativa) cultivars with differing arsenic accumulation factor (AF) values was investigated in an arsenic hotspot in Bangladesh. The results showed that arsenic AF was important in identifying arsenic-safer rice cultivars for growing in an arsenic hotspot. The study based on silicon effect on arsenic uptake in lettuce showed that arsenic accumulation in lettuce (Lactuca sativa) could be reduced by silicon addition. The aquatic moss had good phytofiltration capacity, with fast arsenic removal of up to 82% from a medium with low arsenic concentration (1 µM). Extraction analysis showed that inorganic arsenic species were firmly bound inside moss tissue. Absorption of arsenic was relatively higher than adsorption in the moss. Regarding effects of different abiotic factors, plants were stressed at low pH (pH 2.5) and arsenic removal rate was lower from the medium, while arsenic efflux occurred in arsenate-treated medium at low (12°C) and high (30°C) temperature regimes. Besides these factors, low oxygenation increased the efficiency of arsenic removal from the medium. Finally, combining W. fluitans as a phytofilter with a lettuce crop on a constructed wetland significantly reduced the arsenic content in edible parts (leaves) of lettuce. Thus W. fluitans has great potential for use as an arsenic phytofilter in temperate regions.

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11

Aykas, Didem P. "The Effect of Temperature, Lecithin Content, Voltage, Resistivity, Viscosity, and Surface Tension on Droplets/cm² During Electrostatic Spraying of Oil". The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1330588156.

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12

Akpolat, Hacer. "The Effect of pH and Temperature on Cabbage Volatiles during Storage". The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1427734475.

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13

Gandy, April Lynne. "THE EFFECT OF PASTEURIZATION TEMPERATURE ON CONSUMER ACCEPTABILITY, SENSORY CHARACTERISTICS, VOLATILE COMPOUND COMPOSITION, AND SHELF-LIFE OF FLUID MILK". MSSTATE, 2007. http://sun.library.msstate.edu/ETD-db/theses/available/etd-07022007-162142/.

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The relationship among consumer acceptability, descriptive sensory attributes, and shelf-life was determined for 2 % milk pasteurized at 77, 79, 82, or 85?aC. Pasteurization temperature had no effect (p>0.05) on shelf-life. Consumers preferred (p<0.05) 79?aC over other treatments on day 0; however, six days post-pasteurization 79?aC milk was only preferred (p<0.05) over 77?aC. Consumers were grouped into eight clusters based on product liking for both day 0 and 6 evaluations. The largest cluster liked all pasteurization treatments, and 79?aC was highly acceptable to all consumers that liked milk. Similar sensory descriptors indicated the end of shelf-life for all pasteurization treatments even though treatments could be differentiated by descriptors on day 0. This research reveals that altering pasteurization temperature from 79?aC may cause a decrease in consumer acceptability to some consumers. Altering pasteurization temperature does not affect shelf-life or sensory descriptors and volatile compound profiles at the end of shelf-life.

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14

Kim, Lesley Yu-Jung. "Effect of increased temperature and decreased food quality on metabolism and growth of an algivorous cichlid, Tropheus duboisi and effect of food habit on the field metabolism of African Cichlids". Wright State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1409625207.

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15

Bangcuyo, Ronald G. "Lingual tactile sensitivity: Effect of age, gender, fungiform papillae density, and temperature". The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1436390197.

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16

Khan, Md Imran Hossen. "Fundamental understanding of cellular water distribution and transport in plant-based food material during drying". Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/121217/1/Md%20Imran%20Hossen_Khan_Thesis.pdf.

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This study was conducted to uncover the spatial distribution of cellular water in plant-based food materials and its transport process during drying. A new experimental and analytical method was developed to investigate the cellular water distribution using NMR-T2 relaxometry and X-ray micro CT. It was revealed that at low temperatures cellular water migrates through diffusion whereas at higher temperature cell water mostly migrates through progressive rupturing of the cell walls. This thesis also investigated the impact of process parameters and characteristics of food material on cellular water distribution, transport and associated morphological changes during drying.

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17

Singh, Amandeep. "Effect of temperature/ethanol on the secondary structure of bovine Apo Alpha-Lactalbumin investigated by FTIR/2D IR correlation spectoscropy". Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=123309.

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Whey proteins play a vital role in the manufacture of food products due to their nutritional value and versatile functional properties. α-Lactalbumin (α-LA) is the second most abundant protein in bovine whey and the most abundant protein of human whey. α-LA is a low-molecular-weight (14.2 kDa) and acidic (pI 4-5) protein that is produced in the lactating mammary glands and has a role in lactose biosynthesis. BAMLET/HAMLET (bovine/human alpha-lactalbumin made lethal to tumor cells) are complexes of α-LA and oleic acid that have been shown to have cytotoxic effects on tumor cells but not on healthy cells. In vitro, it has been reported that BAMLET-type complexes can be prepared by heating a solution of bovine apo (calcium-depleted) α-LA in sodium phosphate buffer to which an ethanol solution of oleic acid has been added. However, the possibility that the presence of ethanol may facilitate the complexation of oleic acid with α-LA by affecting the thermal denaturation of the protein has not been investigated In the present study, the combined effects of ethanol and temperature on the secondary structure of bovine apo α-LA were examined by variable-temperature Fourier transform infrared (VT-FTIR) spectroscopy in conjunction with Fourier self-deconvolution (a resolution enhancement technique) and two-dimensional cross correlation spectroscopy (2D CCS). At room temperature, an increase in α-helical and β-structure content at the expense of 310-helices and turns was observed as a function of increasing the concentration of ethanol (from ~2.5 to 33% w/v). These findings are consistent with the fluorescence and proteolysis studies of α-LA reported in the literature, which showed a similar effect of ethanol on the secondary structure of α-LA. Subjecting bovine apo α-LA solutions to a heating–cooling cycle (heating from 25 to 950C and cooling from 95 to 250C) in the presence of varying concentrations of ethanol was found to alter the protein's secondary structure. At any concentration of ethanol the α-helix and 310-helices of the secondary structure of bovine apo α-LA were lost upon heating of the protein. The sequences of the changes in secondary structure during the heating and cooling cycles were elucidated by 2D CCS. The results revealed that the protein refolded during the cooling cycle by reversal of the sequence of unfolding events during the heating cycle only in the presence of 20% or higher concentration of ethanol. Overall, the present study supported the ethanol-induced reversible thermal denaturation of bovine apo-α-LA.
Étant donné leur valeur nutritive et leurs multiples propriétés fonctionnelles, les protéines du lactosérum jouent un rôle crucial dans la fabrication de denrées alimentaires. L'α-lactalbumine (α-LA) est la plus abondante protéine parmi celles du lactosérum bovin et la seconde plus abondante protéine parmi celles du lactosérum humain. L'α-LA , une protéine acide (pI 4-5) à faible poids moléculaire (14.2 kDa) produite dans les glandes mammaires, joue un rôle important dans la biosynthèse du lactose. Composés des complexes d' α-LA et d'acide oléique, le BAMLET/HAMLET (bovine/human alpha-lactalbumin made lethal to tumor cells) a démontré des effets cytotoxiques sur les cellules tumorales, tout en laissant les cellules saines indemnes. On rapporte que les complexes de type BAMLET peuvent être préparées en chauffant une solution d'apo (décalcifiée) α-LA bovine dans un tampon de phosphate de sodium à laquelle on ajoute une solution d'acide oléique dissoute dans de l'alcool éthylique. Cependant, la possibilité que la présence d'alcool éthylique facilite la complexation de l'acide oleique acid à l'α-LA en influençant la dénaturation thermique de la protéine n'a pas été évaluée. Dans la présente étude, les effets combinés de l'alcool éthylique et de la température sur la structure secondaire de l'apo α-LA bovine furent examinés par spectrométrie infrarouge à transformée de Fourier opérant à température variable (VT-FTIR) en combinaison avec une autodéconvolution de Fourier (technique d'amélioration de la résolution) et de spectroscopie à corrélation croisée en deux dimensions (2D CCS). À la température de la pièce, une augmentation de la concentration en alcool éthylique d'environ 2.5% à 33% w/v donna lieu à une augmentation des structures en hélices alpha et à feuillets bêta, aux dépends d'hélices et coudes 310. Ces résultats sont conformes à ceux de plusieurs études fluoroscopiques et protéolytiques de l'α-LA préalables, montrant un effet semblable de l'alcool éthylique sur la structure secondaire de l'α-LA. Soumettant l'apo α-LA bovine à un cycle de chauffage-refroidissement entre des extrêmes de 25°C et 95°C, en présence de différentes concentrations d'alcool éthylique, s'avéra modifier la structure secondaire de la protéine. A toutes concentrations d'alcool éthylique, les hélices alpha et 310 de la structure secondaire de l'apo α-LA bovine furent perdues lorsque la protéine fut chauffée. La séquence des altérations de la structure secondaire durant les cycles de chauffage-refroidissement fut suivie par 2D CCS. Les résultats démontrèrent que ce fut seulement en la présence d'une concentration de 20% ou plus d'alcool éthylique que la protéine se replia durant le refroidissement, en suivant une séquence inverse à celle de son dépliement durant le réchauffement. Globalement, la présente étude confirma la dénaturation thermale réversible de l'apo-α-LA bovine comme étant induite par l'alcool éthylique.

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18

Dixon, Mark Geoffrey. "The effect of temperature and photoperiod on the digestive physiology of the South African abalone Haliotis midae". Thesis, Rhodes University, 1992. http://hdl.handle.net/10962/d1005120.

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Inadequate information of the nutritive physiology and the dietary requirements of abalone are the principle factors that currently limit the development of a formulated feed for the commercial culture of Haliotis midae. The need to develop a method to determine apparent digestibility co-efficient's for abalone in order to facilitate further applied nutritional research was identified. Animals between 50 and 80 mm were collected from natural stocks along the east Cape coast of South Africa at Port Alfred and Great Fish point, and acclimated to laboratory conditions. Initial trials demonstrated that H. midae accepted and preferred a semi-purified diet to the seaweed Plocamium corallorhiza, one of the main components of it's natural diet. A technique of determining apparent digestibility co-efficient's (ADC) using the indirect method with chromic oxide as an inert marker was developed. Digestibility trials yielded higher dry matter (DMADC) and crude protein apparent digestibility co-efficient's (CPADC) for the semi-purified diet than for two species of algae, Gelidium amanzii and P. corallorhiza (83.7% and 95.6%, 70.7% and 80.0%, and 29.9% and 57.3% respectively). The ability of the animals to utilize terrestrial animal and plant ingredients efficiently makes it feasible to use conventional feed ingredients in formulated feeds for H. midae. Trials to determine the effect of different temperatures (15°C, 18°C and 22°C) on DMADC and CPADC of the semipurified diet showed that peak digestibility occurred at 18°C. There was also a positive relationship between temperature and consumption rate. Although no enzyme studies with H. midae have been conducted, the peak ADC's at 18°C is attributed to an increase in enzyme activity at this temperature. Transit time, an inverse function of temperature and consumption, is considered to be responsible for the decrease in the ADC' s at 22°C in conjunction with a possible decrease in enzyme activity at this temperature. A photoperiod trial to investigate the effect of darkness on DMADC and CPADC of the semi-purified diet revealed that digestive efficiency decreased with increasing hours of darkness. There was also a positive relationship between duration of darkness and the rate of consumption. The decrease in ADC's is attributed to decreased transit times as the duration of darkness increased . The contribution of this project to the understanding of abalone nutrition, the development of a formulated abalone feed and systems design for abalone farms is discussed.

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19

Parin, Harika. "Effect Of Pretreatment And Air Temperature On The Drying Rate, Rehydration Capacity And Color Of Artichoke". Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/12605458/index.pdf.

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In this study, cleaned artichoke hearts belonging to three different ages were dried under constant external conditions at 50, 60 and 70°
C using an air inlet velocity of 8.1 m/s. The sample to be dried was pretreated either by keeping it in distilled water or 1% (w/v) ascorbic acid or sodium bisulfite solutions for 30 minutes at the corresponding drying temperatures. Further, for comparison, the use of citric acid solution, increasing the concentrations of the solutions, reducing the pretreatment time, effect of degree of trimming and halving the samples were investigated. The experimental drying rate data were treated to estimate the effective diffusivities and the effect of temperature together with the activation energy according to an Arrhenius type relation. For the product quality, rehydration capacity of the dried samples in water at 20°
C as well as their color were determined. As expected, the rate results indicated an increase in the drying rate hence the effective diffusivity with temperature for the distilled water and ascorbic acid pretreated samples. However, a reduction in the rate at the high drying temperature when sodium bisulfite solution used was attributed to the clogging of the pores by the precipitated solid due to rapid evaporation at the surface. Similarly, rehydration capacity and color of the water treated samples were enhanced with temperature where with the solution treated ones a reverse effect was observed. It is also found that the rehydration data could be well represented by Peleg equation. Further, when citric acid solution was used for pretreatment, the results were quite identical to those of ascorbic acid. Also, increasing the ascorbic acid concentration to 2% (w/v) improved color whereas decreasing the dipping time increased discoloration. Finally, as an important parameter, the degree of trimming of the hearts proved to be highly effective on the rate and the other studied parameters as well as the post harvest and storage time.

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20

Pfunde, Cleopatra Nyaradzo. "Parent characterization of quality protein maize (Zea mays L.) and combining ability for tolerance to drought stress". Thesis, University of Fort Hare, 2012. http://hdl.handle.net/10353/d1007536.

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Quality protein maize (QPM) has enhanced levels of two essential amino acids, lysine and tryptophan compared to normal maize. This makes QPM an important cereal crop in communities where maize is a staple crop. The main abiotic factor to QPM production is drought stress. Little information is available on the effect of drought stress on QPM. Therefore, the objectives of this study were to: (i) conduct diversity analysis of QPM inbred lines using morpho-agronomic and simple sequence repeat markers, (ii) screen available QPM inbred lines and F1 progeny for tolerance to seedling drought stress, (iii) determine the combining ability and type of gene action of QPM inbred lines for tolerance to seedling drought stress, grain yield and endosperm modification. The study was conducted in South Africa, at the University of Fort Hare. Morphological characterisation of 21 inbred lines was done using quantitative and qualitative traits. A randomised complete block design with three replicates was used for characterizing the inbred lines in the field. Genstat statistical software, version 12 (Genstat ®, 2009) was used for analysis of variance (ANOVA) and descriptive statistics. Analysis of variance was performed on all quantitative data for morphological traits. Data for qualitative traits was tabulated in their nominal classes. Traits that contributed most to the variation were days to anthesis, days to silking, anthesis-silking interval, plant height, number of kernel rows, ear length and grain yield. Cluster analysis grouped the inbred lines into three main clusters. The first cluster was characterised by tall and average yielding lines, while the second cluster showed the least anthesis-silking interval, and had the highest yield. Cluster three consisted of lines that were early maturing, but were the least yielding. Genetic distances between maize inbred lines were quantified by using 27 simple sequence repeat markers. The genetic distances between genotypes was computed using Roger’s (1972) genetic distances. Cluster analysis was then carried out using the neighbour-joining tree method using Power Marker software version 3.25. A dendrogram generated from the genetic study of the inbred lines revealed three groups that concurred with expectations based upon pedigree data. These groups were not identical to the groups generated using morpho-agronomic characterisation. Twenty one QPM inbred lines were crossed using a North Carolina design II mating scheme. These were divided into seven sets, each with three inbred lines. The three inbred lines in one set were used as females and crossed with three inbred lines in another set consisting of males. Each inbred line was used as a female in one set, and as a male in a second set. Sixty three hybrids (7 sets x 9 hybrids) were formed and evaluated in October 2011, using a 6x8 alpha-lattice incomplete block design with three replicates under glasshouse and optimum field conditions. A randomised complete block design with three replicates was used for the 21 parental inbred lines. Traits recorded for the glasshouse study were, canopy temperature, chlorophyll content, leaf roll, stem diameter, plant height, leaf number, leaf area, fresh and dry root and shoot weights. Data for the various traits for each environment, 25 percent (stress treatment) and 75 percent (non-stress) of field capacity, were subjected to analysis of variance using the unbalanced treatment design in Genstat statistical package Edition 12. Where varietal differences were found, means were separated using Tukey’s test. Genetic analyses for grain yield and agronomic traits were performed using a fixed effects model in JMP 10 following Residual Maximum Likelihood procedure (REML). From the results, inbred lines that were not previously classified into heterotic groups and drought tolerance categories were classified based on their total dry weight performance and drought susceptibility index. Inbred lines L18, L9, L8, L6 and L3, in order of their drought tolerance index were the best performers under greenhouse conditions and could be recommended for breeding new varieties that are tolerant to seedling drought stress. Evaluation of maize seedlings tolerant to drought stress under glasshouse conditions revealed that cross combination L18 x L11 was drought tolerant, while cross L20 x L7 was susceptible. Total dry weight was used as the major criteria for classifying F1 maize seedlings as being resistant or susceptible. General combining ability effects accounted for 67.43 percent of the genetic variation for total dry weight, while specific combining ability effects contributed 37.57 percent. This indicated that additive gene effects were more important than non-additive gene action in controlling this trait. In the field study (non-drought), the experimental design was a 6x8 alpha lattice incomplete block design with three replicates. On an adjacent field a randomised complete block design with three replicates was used to evaluate the parental inbred lines. The following variables were recorded: plant height, ear height, ears per plant, endosperm modification, days to silking and days to anthesis, anthesis-silking interval, number of kernels per row, number of rows per ear and grain yield. General analyses for the incomplete lattice block design and randomised complete block design for hybrid and inbred data respectively were performed using JMP 10 statistical software. Means were separated using the Tukey's test. Genetic analyses of data for grain yield and agronomic traits were conducted using a fixed effects model using REML in JMP 10. The importance of both GCA (51 percent) and SCA (49 percent) was observed for grain yield. A preponderance of GCA existed for ear height, days to anthesis, anthesis-silking interval, ears per plant and number of kernels per row, indicating that predominantly, additive gene effects controlled hybrid performance under optimum field conditions. The highest heritability was observed for days to silking (48.27 percent) suggesting that yield could be improved through selection for this trait. Under field conditions, variation in time to maturity was observed. This implies that these inbred lines can be recommended for utilisation in different agro-ecologies. Early maturing lines such as L18 can be used to introduce earliness in local cultivars, while early maturing single crosses such as L18 x L2, L5 x L9, L3 x L4 and L2 x L21 could be recommended for maize growers in drought prone areas such as the former Ciskei. Single crosses L18xL11, L16xL18, L8xL21 and L9xL6 had good tolerance to seedling drought stress. On the other hand, single crosses L18xL11 and L11xL13 had high grain yield and good endosperm modification. All these single crosses could be recommended for commercial production after evaluation across locations in the Eastern Cape Province. Alternatively they can be crossed with other superior inbreds to generate three or four way hybrids, which could then be evaluated for potential use by farmers in the Eastern Cape.

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21

Shikwambana, Kingsly. "Effect of harvest time, post-harvest storage and ripening temperature on fruit quality of reed avocado cultivar". Thesis, University of Limpopo, 2016. http://hdl.handle.net/10386/2049.

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Thesis (M. Sc. (Agriculture, Horticulture)) --University of Limpopo, 2016
‘Reed’ avocado is a late season cultivar introduced to South Africa from California. The cultivar has shown good adaptation and produces quality fruit with export potential. Its pre-harvest adaptation and production aspects have been researched and documented. However, the effect of harvest time, post-harvest storage and ripening temperature has not been comprehensively studied on this newly introduced ‘Reed’ avocado cultivar. Therefore, the aim of this work was to investigate the effect of different harvest time, post-harvest storage and ripening temperature on the quality of late season ‘Reed’ avocado fruit. Matured ‘Reed’ avocado fruit were harvested based on moisture content indexing in December (2015) and January (2016). The experiment was carried out in a factorial, arranged in a completely randomised design (CRD) with three replicates. Treatment factors were: 2 x harvest time (mid-and late), 2 x post-harvest storage (2.0 and 5.5°C), 3 x ripening temperature (16, 21 and 25°C) and 5 x ripening day (0, 2, 4, 6 and 8). Fruit were stored at 2.0 and 5.5°C for 28 days, thereafter, ripened at 16, 21 and 25°C until fully ripe. During ripening, fruit were evaluated for weight loss, skin colour, firmness, respiration rate, physiological and pathological disorders. Mid-harvest fruit had higher moisture content when compared with late harvest fruit. However, harvest time, post-harvest storage, ripening temperature and ripening time (days) significantly influenced fruit weight loss, firmness, respiration rate, ripening percentage of ‘Reed’ avocado fruit during ripening. Moreover, fruit firmness decreased faster at higher temperatures (25 and 21°C) with fruit ripening within 4 and 6 days, respectively. In addition, ripening at a lower temperature (16°C) was slower with fruit fully ripened within 8 days after withdrawal from cold storage at both harvest times. ‘Reed’ avocado fruit respiration rate followed a climacteric pattern, however, significantly higher rate at higher temperature (25°C) when compared with lower temperature (16°C) after withdrawal from cold storage during both harvest times. Interestingly, mid-harvest fruit showed high electrolyte damage after withdrawal from 2.0°C when compared with late harvest fruit at the same temperature. Furthermore, mid-harvest fruit stored at 2.0ºC and ripened at 21°C showed higher chilling injury when compared with fruit ripened at 16 and 25°C. High electrolyte leakage positively correlated (R2 = 0.242) with high chilling damage for xiv mid-harvest fruit stored 5.5°C. Treatment factors had a significant effect (P < 0.05) on lightness (L *) and hue angle (h ) but no significant effect (P > 0.05) on chroma (C *) and eye colour of ‘Reed’ avocado fruit during ripening, irrespective of harvest time. Overall results showed a visual change in ‘Reed’ avocado skin colour, with eye colour changing from green to bright yellow. Furthermore, late harvest fruit showed high post-harvest pathological diseases after removal from high temperature (5.5°C) when compared with mid-harvest fruit stored at low storage temperature (2.0°C). Ripening at a higher temperature (21 and 25°C) resulted in higher incidence of stem-end rot and body rot when compared with lower temperature (16°C) for both harvest times. Late harvest fruit showed a higher incidence of vascular browning at higher ripening temperatures (21 and 25°C) when compared with lower temperature (16°C) after withdrawal from cold storage. Moreover, overall results showed that harvest time, post-harvest storage and the ripening temperature had a profound influence on the quality of ‘Reed’ avocado fruit. In conclusion, ‘Reed’ avocado fruit can be harvested during mid- or late season and stored at recommended low temperature (2.0ºC); and thereafter, ripened at either 16 or 21ºC. In addition, future studies should focus on identifying pre-harvest practices that promote higher post-harvest fruit quality for ‘Reed’ avocado fruit under South African production environment. Keywords: ‘Reed’ avocado fruit; firmness; electrolyte leakage; respiration rate; stem-end rot; body rot; vascular browning

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22

Erwee, Anton. "Development of technology for the production of stable high moisture dried fruit". Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53365.

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Thesis (MSc Food Sc )--Stellenbosch University, 2003.
ENGLISH ABSTRACT: Dried fruit is a well-known food product that has been produced for many years. The product characteristics have remained constant throughout this time with a moisture content of ca. 18 - 26% (mlm). However, in recent times there has been a definite trend towards a fmal dried fruit product in the intermediate moisture range with a moisture content of ca. 36%. The high water activity (aw) of the product (ca. 0.85) makes the product susceptible to microbiological spoilage and the product therefore requires a subsequent pasteurisation step to ensure a safe product. A further consequence of the increased moisture content, higher aw and the temperature of the pasteurisation step, is the increased rate of non-enzymatic browning reactions. Currently the shelf life is only 15 weeks while a minimum shelf life of 30 weeks is required to enable product export. Moisture sorption isotherms may be used to depict the relationship between moisture content and aw. Moisture sorption isotherms were thus determined for Royal type apricots and nectarines at 25° and 40°C. Samples, equilibrated at relative humidities in the range of 11-97%, were obtained using saturated salt solutions and a static gravimetric method. Isotherms were found to be of type I, typical of dried fruit. Six mathematical models namely; BET, GAB, Iglesias and Chirife, Halsey, Henderson, and Chung and Pfost, were fitted to experimental data. The GAB model predicted the aw of both apricots and nectarines the best at 25° and 40°C with the Henderson equation second best in all instances. The binding energy, as a function of moisture content, was calculated to determine energy requirements for drying. At low moisture contents « 20%) an increase of energy was required for drying. Discolouration of macerated dried Royal type apricots and nectarines during accelerated storage as affected by moisture (32, 36, and 40%, mlm) and sulphur dioxide (S02) content (2500, 3000 and 3300 mg.kg" for apricots; 1800, 2200 and 2600 mg.kg" for nectarines) was investigated. The macerated samples were stored at 30°, 40°, 50° and 60°C. Colour was quantified in terms of the L* value of the CIELab system (used throughout the study). Moisture and S02 contents affected both the initial fruit colour and the rate of discolouration. The highest L* values, i.e. lightest fruit colour, were obtained for fruit at 40% moisture content and the highest S02 levels. Increasing storage temperature accelerated the loss of moisture and S02. The influence of a 10°C increase in storage temperature on the rate of browning and thus shelf life was described in terms of the QIO value. QIO and aw values of apricots ranged from 1.96 - 2.47 and 0.833 - 0.890, respectively, while QIO values of 1.50 - 4.61 and aw values ofO.844 to 0.890 were obtained for nectarines. Discolouration of dried nectarine halves during accelerated storage at 40°C as affected by rehydration method, moisture content, packaging atmosphere and pasteurisation method, was investigated. The fruit halves were rehydrated using three different methods to obtain moisture contents of 36 and 40%, respectively. Dry heat and steam pasteurisation techniques were used to render a microbiologically safe product. Commercial packaging material was used and the atmosphere was modified with CO2 to lower the O2 concentration in the headspace. A two-step rehydration at 45°C, steam pasteurisation at 90°C for 150 minutes and packaging under a high CO2 atmosphere rendered a product with the best colour retention under accelerated storage conditions of 40°C for eight weeks. To confirm the results obtained with accelerated storage at temperatures that the product would normally be retailed at, shelf life tests were also performed at 5° and 25°C. Discolouration of whole dried Royal type apricot and nectarine halves as affected by rehydration method, moisture content, packaging atmosphere and pasteurisation method was investigated. The methodology for rehydrating, pasteurising and packaging the high moisture dried fruit developed in this study was compared against the standard method used by the industry. The new processing method increased shelf life. Samples were stored for a period of 30 weeks and were tested every five weeks to determine CO2 concentration in headspace, colour retention and S02 concentration of the fruit. Both apricots and nectarines achieved a shelf life of 30 weeks at both storage temperatures and an extrapolated shelf life of 89 weeks at 5°C, but only 32 weeks at 25°C.
AFRIKAANSE OPSOMMING: Droë vrugte is 'n welbekende voedselproduk en word reeds vir baie jare vervaardig. Die produkeienskappe het konstant gebly gedurende hierdie tydperk met 'n produkvoginhoud van ca. 18 - 26% (mlm). Daar is egter 'n tendens die afgelope tyd na 'n finale produk in die intermediêre voggebied met 'n voginhoud van ca. 36% en 'n water aktiwiteit (aw) van ca. 0.85. Hierdie verandering in voginhoud en aw maak die produk vatbaar vir mikrobiologiese bederf, en gevolglik word pasteurisasie benodig om dit te preserveer. 'n Verdere gevolg van die verhoogde voginhoud en aw en die hoë temperature van pasteurisasie, is die verhoogde tempo van nieensiematiese verbruiningsreaksies. Huidig is die produk se rakleeftyd 15 weke terwyl 'n minimum van 30 weke benodig word om hierdie produk suksesvol uit te voer. Vogsorpsie-isoterme kan gebruik word om die verwantskap tussen voginhoud en aw uit te beeld. Vogsorpsie-isoterme van Royal tipe applekose en nektariens is gevolglik bepaal by 25° en 40°C. Monsters, geëkwilibreer by relatiewe humiditeite van 11 - 97%, is verkry deur gebruik te maak van versadigde soutoplossings en 'n statiese gravimetriese metode. Tipe I isoterme, wat tipies van droëvrugte is, is verkry. Ses wiskundige modelle naamlik; BET, GAB, Iglesias en Chirife, Halsey, Henderson, en Chung en Pfost, is gepas op die data. Die GAB model het die aw van beide appelkose en nektariens by 25° en 40°C die beste voorspel en die Henderson model die tweede beste in al die gevalle. Die bindingsenergie as 'n funksie van voginhoud is bereken om die energie vereistes van droging te bepaal. By lae voginhoude (<20%) is 'n skerp styging in benodigde energie waargeneem. Die verkleuring van gemaalde gedroogde Royal tipe appelkose en nektariens gedurende versnelde opberging en die invloed van voginhoud (32, 36, en 40%, mlm) en swaweldioksied (S02) konsentrasie (2500, 3000 en 3300 mg.kg' vir appelkose; 1800, 2200 en 2600 mg.kg" vir nektariens) is ondersoek. Die gemaalde monsters is gestoor by 30°, 40°, 50° en 60°C. Kleur is gekwantifiseer in terme van L* waardes van die CIELab sisteem (ook gebruik vir daaropvolgende ondersoeke). Vog en S02 het albei die aanvanklike kleur asook die tempo van verbruining beïnvloed. Die hoogste L* waardes, d.i. die ligste kleur, is verkry vir die monsters met 40% voginhoud en die hoogte S02 vlakke. Verhoogde temperature tydens opberging het aanleiding gegee tot verhoogde verliese van vog en S02. Die invloed van 'n 10°C verhoging in opbergingstemperatuur op die tempo van verbruining en dus rakleeftyd, word beskryf in terme van QIO waardes. QIO en aw waardes van die appelkose het gestrek van 1.96 - 2.47 en 0.833 - 0.890, onderskeidelik, terwyl QIO waardes van 1.50 - 4.61 en aw waardes van 0.844 tot 0.890 verkry is vir die nektariens. Verkleuring van gedroogde nektarien halwes gedurende versnelde opberging by 40°C en die invloed van rehidrasie metode, voginhoud, verpakkingsatmosfeer en pasteurisasiemetode is ondersoek. Die vrughalwes is gerehidreer deur middel van drie metodes om die voginhoud te verhoog tot 36 en 40%, onderskeidelik. Droë hitte en stoompasteurisasie metodes is gebruik om 'n mikrobiologiese veilige produk daar te stel. Kommersiële verpakkingsmateriaal is gebruik en die CO2 konsentrasie van die atmosfeer in die verpakking is verhoog om die invloed daarvan te bepaal. 'n Twee-stap-rehidrasie by 45°C, stoompasteurisasie by 90°C vir 150 minute en 'n hoë C02 atmofeer het aanleiding gegee tot die monster met die beste kleurbehoud tydens versnelde opberging by 40°C vir agt weke. Om die resultate, verkry met die versnelde rakleeftyd studie, te bevestig by temperature waarby die produk normaalweg blootgestel sal word tydens kleinhandel, is 'n rakleeftyd studie uitgevoer by 5° en 25°C. Verkleuring van heel gedroogde Royal tipe appelkoos en nektarien halwes, die invloed van rehidrasie metode, voginhoud, verpakkingsatmosfeer en pasteurisasiemetode is ondersoek. Die metodiek vir die rehidrasie, pasteurisasie en verpakking van hoë vog droëvrugte ontwikkel in hierdie studie, is getoets teen die standaardmetode wat deur die industrie gebruik word. Die nuwe prosesseringsmetode het aanleiding gegee tot 'n langer rakleeftyd. Monsters is opgeberg vir 'n tydperk van 30 weke om die rakleeftyd te bepaal. Die CO2 konsentrasie in die pakkie, kleurbehoud en S02 konsentrasie van die vrugte is elke vyf weke getoets. Beide appelkose en nektariens het 'n rakleeftyd van 30 weke by albei opbergingstemperature behaal, terwyl 'n ekstra-gepoleerde rakleeftyd van 89 weke by 5°C en 32 weke by 25°C behaal is.

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Kritzinger, Madeleine. "Evaluation of hot water and hot air heat shock treatments on South African avocados to minimise the occurrence of chilling injury". Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52835.

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Thesis (MSc Food Sc)--Stellenbosch University, 2002.
ENGLISH ABSTRACT: The South African avocado fruit industry is export driven and the successful storage of fruits for extended periods is, therefore, essential. It was recorded that 7.7 million cartons were exported during the 1995 season. The shipping of the avocados takes approximately 15 days and the fruits are being stored at low temperatures to minimise the possibility of fruits softening. Unfortunately low temperature storage results in chilling injury. A possible method to increase avocado resistance to chilling injury is to administer a heat shock treatment. In this way the fruits are protected from chilling injury by inducing the formation of so-called heat-shock proteins which render the cell membranes more resistant to chilling injury. The objective of this study was to evaluate different heat shock treatment protocols as a method of preventing or minimising chilling injury and to extend the shelf-life of avocado fruits while exporting at the lowest possible temperature. Examining the effect of different temperatures and exposure times on the quality of the different avocado cultivars pursued this. The exterior chilling injury on each fruit was quantified and the firmness and internal quality parameters evaluated. A total of 32 Experimental Studies were conducted. The results showed that the Hot Water Heat shock Treatment (HWHST) worked effectively for the South African 'Fuerte' cultivar between 40° and 42°C for exposure times of between 20 and 30 min. The 'Edranol' cultivar also showed promising results between 40° and 42°C for exposure time of between 8 and 22 min. The HWHST was not effective on the South African 'Hass' cultivar. The 'Ryan' cultivar with its thick skin made this cultivar less susceptible to chilling injury and therefore HWHST would be unnecessary. The 'Pinkerton' cultivar had a lot of factors that influenced the results. Therefore, more research needed to be done on the 'Pinkerton' cultivar, before any conclusions could be obtained from this cultivar, although it showed potential. Hot air treatment worked fairly well, but unfortunately the long exposure time needed made this treatment unpractical. Throughout the whole study the importance of maturity surfaced as a major role in all the aspects of post harvest quality.
AFRIKAANSE OPSOMMING: Die Suid-Afrikaanse avokado vrugtebedryf is hoofsaaklik gerig op die uitvoermark en daarom is dit belangrik dat die vrugte vir 'n bepaalde tyd suksesvolopgeberg kan word. Die sensus opname gedurende die 1995 seisoen het getoon dat 7.7 miljoen bokse avokados uitgevoer is. Die avokados word vir ongeveer 15 dae per boot vervoer, wat kan lei tot vrugte wat sag word. Om dit te verhoed, word die vrugte by lae temperature opgeberg. Ongelukkig veroorsaak lae opbergingstemperature koueskade. 'n Moontlike metode om avokados te beskerm teen lae temperature en koueskade te verminder, is om 'n hitteskok behandeling toe te pas. Op hierdie manier word die vrugte beskerm teen koueskade deur die vorming van sogenaamde hitteskok proteïene wat die selwande meer bestand maak teen koueskade. Die doel van hierdie studie was om die verskillende hitteskok behandelings protokols te evalueer as 'n metode van beskerming of vermindering van koueskade en om sodoende die rakleeftyd van avocados te verleng as die vrugte by lae temperature uitgevoer word. Eksperimente is uitgevoer om die effek van verskillende temperature en blootstellingstye op die kwaliteit van die verskillende avokado kultivars te bepaal. Die koueskade op die oppervlakte van elke vrug is bepaal en die fermheid en interne kwaliteit parameters is geëvalueer. In totaal is daar altesaam 32 Eksperimentele Studies gedoen. Die resultate het gewys dat die Warm Water Hitteskok Behandeling (WWHB) effektief was op die Suid-Afrikaanse 'Fuerte' kultivar by temperature tussen 40° en 42°C en by blootstellingstye van tussen 20 en 30 min. Belowende resultate is ook met die 'Edranol' kultivar by temperature tussen 40° en 42°C met blootstellingtye van tussen 8 en 22 min behaal. Die WWHB was oneffektief vir die Suid-Afrikaanse 'Hass' kultivar. Die 'Ryan' kultivar se dik skil het hierdie kultivar minder vatbaar gemaak vir koueskade en daarom was 'n WWHB onnodig gewees. By die 'Pinkerton' kultivar kon daar nog nie 'n gevolgtrekking gemaak word nie, aangesien daar nog baie faktore is wat ondersoek moet word, alhoewel die kultivar baie potentiaal getoon het. Warm lug behandeling het potensiaal gehad, maar die lang blootstellingstye het hierdie behandeling onprakties gemaak. Gedurende die hele studie is daar klem gelê op die rypheisgraad van die vrugte wat na vore gekom het as 'n belangrike faktor wat 'n hoofrol speel in al die aspekte van die na-oes kwaliteit.

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24

Milham, Paul J. "The behaviour of cadmium in soil". Thesis, View thesis, 2008. http://handle.uws.edu.au:8081/1959.7/39519.

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Long-term low-level ingestion of cadmium (Cd) causes human health problems, and in Australia, vegetables supply ~40% of the Cd in the typical diet. Plants take up Cd from the soil; however, the uptake is poorly predicted by simple soil tests, such as the total concentration of Cd (Cdt). Therefore, a greater understanding of Cd behaviour in soils is needed to improve the prediction of Cd uptake by plants and open a new path to minimise the risks for human health. The objectives of the research in this thesis were to: identify key soil properties affecting Cd behaviour, identify/develop selective methods to measure them, and to formulate a conceptual model of Cd partitioning. These objectives were based on the hypothesis that empirical modelling informed by a better understanding of Cd chemistry would accurately describe Cd partitioning in soil. To test the hypothesis, the key properties were measured on soils from the peri-urban fringe of Greater Sydney (n = 41) and a series of models of increasing complexity were fitted to the data. A model with three explanatory variables— log10 Cdt, pH and log10 ECEC (effective cation exchange capacity)—explained 94.6% of variation in log10 CdCa (the concentration of Cd in solution in a suspension of soil in 10 mM CaCl2), which strongly supported the hypothesis. The study also indicated that the explanatory variables, Cdt, pH and ECEC, may describe Cd behaviour in many soils, and that for these general models, partition coefficients, such as log10 (Cdt/CdCa), are unsuitable dependent variables. The preceding model used Cdt as an explanatory variable, notwithstanding that labile Cd (CdE) was mechanistically preferable. However, CdE can only be measured using isotopic techniques: a requirement that has constrained the evaluation of CdE as an index of Cd behaviour and bioavailability. Therefore, a simple proxy measure of CdE was investigated. The literature indicated that solutions of chloride salts might selectively extract CdE, and Cd extracted into 1 M NH4Cl (CdNH4Cl) was compared with CdE measured by stable isotope dilution ICPMS. For 23 soils from the partitioning study, 1 M NH4Cl failed to completely extract CdE, unless the pH was less than 5. The cause(s) of this effect will be investigated with the aim of developing a universally applicable measure of CdE that does not require isotopic measurements. All models of Cd uptake by plants rely on soil properties measured on homogenised samples, although the distribution and bioavailability of Cd vary spatially in the field. Were such variability to increase at the micro-scale, its effects could erode the accuracy with which models could predict Cd behaviour and uptake. Consequently, I tested whether the distribution of Cd could be mapped by using synchrotron micro-x-ray fluorescence spectroscopy (micro- XRFS): the most sensitive method of observation. The soils examined contained 0.3–6.4 mg Cd/kg, i.e. were typical agricultural soils, and one was spiked to ~100 mg Cd/kg. Micro-XRFS mapped the Cd in the spiked soil, and in one particle in the other soils. For typical agricultural soils, the sensitivity realised in this study would have been sufficient to characterise the average Cd binding site, but fell at least 10-fold below that needed to map the Cd distribution in them. The research satisfied the objectives, advanced knowledge of Cd behaviour in soils, and provided new research leads. These leads include the possibility of developing general models of Cd partitioning in soils, derivatives of which may predict Cd uptake by plants. The accuracy of these models may be strengthened by the use of CdE as an explanatory variable, but may be weakened by the effects of in situ variation in the distribution of Cd. The benefits to human health of agricultural practices that decrease dietary Cd justify continuation of research to develop models that accurately predict Cd uptake by plants.

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25

Linton, Richard Howard. "Use of the Gompertz equation to model non-linear survival curves and predict temperature, pH, and sodium chloride effects for Listeria monocytogenes Scott A". Diss., This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-164544/.

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Botha, Sune St Clair. "The effects of hot-deboning on the physical quality characteristics of ostrich (Struthio camelus) Muscularis gastrocnemius, pars interna and Muscularis iliofibularis". Thesis, Stellenbosch : University of Stellenbosch, 2005. http://hdl.handle.net/10019.1/1513.

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Thesis (Msc Food Sc (Food Science))--University of Stellenbosch, 2005.
The aim of this study was to investigate the effects of hot-deboning (1 h post-mortem) on the shelf-life and the physical meat quality characteristics, including tenderness, pH, purge (%), cooking loss (%), and raw meat colour of vacuum packed ostrich (Struthio camelus var. domesticus) meat cuts from the M. gastrocnemius, pars interna and the M. iliofibularis during post-mortem refrigerated aging for respectively 21 d at 4ºC and 42 d at -3º to 0ºC.

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27

Di-tanno, Marilisa Flavia Pereira. "Influência da tempertura, tempo e concentração de pectinase na textura, rendimento e características físico-químicas da mandioca (Manihot esculenta C.) durante fermentação". Universidade de São Paulo, 2001. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-03072002-174015/.

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Pubagem é o processo de fermentação natural de raízes de mandioca para produção de puba, um alimento tradicional nas regiões Norte e Nordeste do Brasil. Além da fermentação lática, uma ação combinada da pectina-metil-esterase endógena e enzimas microbianas despolimerizantes causa o amolecimento das raízes, que é importante para sua completa desintegração. No entanto, o reconhecimento do ponto final de fermentação e as condições que propiciam puba de boa qualidade não são completamente esclarecidos. O objetivo do trabalho foi estudar a influência da temperatura de fermentação e da adição de enzima como auxiliar de pubagem na textura das raízes e rendimento de puba, procurando associar estes parâmetros para detectar o ponto final desta etapa do processo. Cerca de 1Kg de raízes descascadas e 2 litros de água foram colocados em recipientes plásticos. Diferentes concentrações (0,1 e 2mL) de pectinase comercial de Aspergillus aculeatus/Kg de raiz foram adicionadas em tratamentos separados. Amostras foram incubadas a 25, 30 e 35ºC, por períodos de 48, 72 e 96 horas. Foram efetuadas análises de acompanhamento do processo fermentativo (pH, textura e rendimento) e da composição da farinha de puba obtida. O maior rendimento, de 77% após 72 horas, foi observado no tratamento a 25ºC com 2mL de enzima por Kg de raiz, quando a textura que era de 18,62lbf/g na matériaprima passou para 3,92lbf/g na raiz pubada. Outros rendimentos mais próximos desse valor, 75,7 e 74,7%, foram obtidos respectivamente na fermentação natural a 35ºC e na amostra tratada com 2mL de enzima incubada a 30ºC/48 horas. Os valores de textura foram de 2,50lbf/g para o primeiro tratamento e 2,89lbf/g para o segundo. A relação entre textura e rendimento foi significativa para a temperatura de 30ºC e todos os períodos de 72 horas. A associação entre os dados de textura e rendimento permite concluir que quando a textura alcança valores da ordem de 4lbf/g os rendimentos de puba são maiores, indicando o final do processo. Os rendimentos mais baixos ocorreram para os valores de textura próximos a 2,30lbf/g de amostra, geralmente obtidos em fermentações mais longas e com maior concentração de enzima.
"Retting" is a natural fermentation of cassava roots for the production of puba, a traditional food in the North and Northeast of Brazil. Besides the lactic fermentation, the combined action of the endogenous pectin-methyl-esterase and depolymeryzyng enzymes, causes a softening of the roots which is important for its complete disintegration. However, the recognition of the end point of fermentation and the conditions that lead to good quality puba are not completely understood. The objective of this work was to study the influence of the temperature and enzyme concentration on the yield and texture, aiming at associating these parameters to detect the end point of fermentation. The physicochemical properties of the puba flour were also determined. About 1Kg of peeled roots and 2 liters of water were placed in plastic containers. One or two milliliters of commercial pectinase from Aspergillus aculeatus were added / Kg root, in separate treatments. In the control treatment no enzyme was added. Samples were incubated at 25, 30 and 35ºC and portions removed after 48, 72 and 96 hours for the determination of pH, texture, yield and dry matter. Fibers, starch, total soluble sugars and amylose were determined in the flour of the dried puba. The highest yield of 77% was obtained after 72 hours for the treatment at 25ºC with 2mL of enzyme/kg of root, when the texture values decreased from 18.62lbf/g (raw material) to 3.92lbf/g (retted root). A high yield of 75.7% was also observed in same period, for the natural fermentation at 35ºC, when texture values of 2.50lbf/g were reached. The relation between texture and yield was significant for a temperature of 30ºC and a period of 72 hours. The association between the texture and yield data suggests that when the texture reaches values of the order of 4lbf/g, the yields are higher, indicating the end of the process. The yields were lower when texture values were close to 2.30lbf/g, usually achieved for long fermentations and higher enzyme concentrations.

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28

Makambila, Casimir. "Etude de l'anthracnose du manioc (manihot esculenta crantz) et son agent pathogene colletotrichum gloeosporioides penz f. Sp. Manihotis henn". Clermont-Ferrand 2, 1987. http://www.theses.fr/1987CLF2E385.

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L'agressivite des souches de colletotrichum manihotis varie d'une souche a une autre et les cultivars de manioc inocules sont plus ou moins sensibles au champignon. La temperature et la lumiere exercent une action sur l'infection des tiges. In vitro la lumiere stimule ou inhibe, selon les temperatures la croissance en longeur des hyphes et la conidiogenese. Les radiations bleues, vertes et jaunes inhibent la croissance a 32 non=c. , les radiations rouges stimulent la croissance a des temperatures comprises entre 20 et 28 non=c. La conidiogenese est inhibee a 20 non=c. Et stimulee a partir de 24 non=c, par des radiations bleues et jaunes. Les radiations rouges stimulent la condiogenese a partir de 24 non=c jusqu'a 28 non=c. Le vert est toujours stimulateur quelle que soit la temperature

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29

Mbande, Sekiwe. "Fishes in the Mngazi and Mngazana estuaries, with particular emphasis on the community structure and primary carbon sources". Thesis, Rhodes University, 2004. http://hdl.handle.net/10962/d1005386.

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The fish community structure of two contrasting estuaries, one with a well developed mangrove forest (Mngazana) and the other without mangroves (Mngazi) was compared. Both the Mngazi and Mngazana estuary fish communities were dominated by marine species, reflecting the importance of these systems as nursery areas for marine fishes. The Mngazi Estuary contained 18% more estuarine fishes in terms of catch per unit effort (CPUE) than the Mngazana Estuary. The reduced tidal influence due to the narrow mouth opening is a possible reason for the heightened CPUE of estuarine species in the Mngazi estuary. The recorded higher diversity of fish species in the Mngazana Estuary when compared with the Mngazi Estuary was attributed to the greater influence of the marine environment due to the wide permanently open mouth, as well as the presence of a variety of habitats in this system. In both estuaries tropical and temperate species were captured, confirming the transitional nature of their biogeographic location which is situated close to the boundary between the subtropical and warm temperate regions of the Southern African coastline. Contrary to previous studies, which recorded seasonal changes in the proportions of tropical and temperate species, the proportions of tropical species remained unchanged at approximately 70% during the January and June sampling occasions. Global warming as a possible reason for the increased dominance of tropical species is discussed. Although several studies in southern Africa have investigated estuarine food web structure, none have compared mangrove and non-mangrove estuaries. In this study, the primary sources of carbon utilised by the fish fauna in the Mngazi and Mngazana estuaries was investigated. The carbon isotopic values of fishes in both estuaries displayed a continuum rather than a tight clustering around particular energy sources. Most detritus feeders of the family Mugilidae (mullets) from both estuaries were relatively more enriched than other fish taxa. The isotopic values of the mullet species suggest a diet derived from relatively enriched carbon sources such as benthic microalgae, the eelgrass Zostera capensis and associated epiphytes. Based on the isotopic values, piscivorous fishes from both estuaries could not be linked to specific prey fish taxa, but clearly the mullet species were not their main food source. The invertebrate feeders that were found in both estuaries showed greater isotopic variations in the Mngazana Estuary than in the Mngazi Estuary, probably reflecting the higher diversity of habitats (carbon sources) and invertebrate prey species in the Mngazana system. Generally the isotopic signatures of fishes from the Mngazi Estuary were more enriched than those from the Mngazana Estuary, thus indicating the possible effect of δ¹³C depleted mangrove derived carbon in the latter system.

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30

Sigrist, José Maria Monteiro. "Estudos fisiológicos e tecnológicos de couve-flor e rúcula minimamente processadas". Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-03042003-155537/.

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Este trabalho teve por finalidade avaliar os efeitos de temperatura nos metabolismos de couves-flores e rúculas inteiras e minimamente processadas e diferentes materiais de embalagem na sua qualidade. No primeiro experimento, couves-flores, seus floretes, rúculas e suas folhas foram mantidos a 1º, 5º e 11ºC e determinados suas taxas de respiração, produção de etileno e quocientes de temperatura (Q10). No segundo, os floretes foram colocados em embalagens de filmes de polietileno de baixa densidade (PEBD), poliolefínicos coextrusados (PD 941 e Clysar) e de policloreto de vinila, PVC esticável de 12 e 20 mm, envolvendo bandejas de poliestireno expandido mantidos a 5ºC. Análises físicas, químicas, físico-químicas, microbiológicas e sensoriais foram realizadas. No terceiro, folhas de rúculas foram colocadas em embalagens de filme de polietileno de baixa densidade (PEBD), laminado de polipropileno/polietileno (PP/PE), poliolefínicos coextrusados (PD 900 e Clysar) e de policloreto de vinila, PVC esticável de 20 mm, envolvendo bandejas de poliestireno expandido e armazenados a 5ºC. Análises físicas, químicas, físico-químicas, microbiológicas e sensoriais foram realizadas. As taxas respiratórias das couves-flores inteiras e minimamente processadas foram iguais em quaisquer das temperaturas estudadas e sempre decrescentes até o 16º dia. A 1º e a 5ºC, tiveram a mesma taxa respiratória, diferindo das mantidas a 11ºC. Na faixa de 1º a 11ºC, o Q10 foi o mesmo para floretes e couves-flores; 2,1 e 2,2, respectivamente. Comportamento distinto tiveram as rúculas inteiras e as folhas soltas, pois, a 1ºC e a 5ºC, as taxas respiratórias foram significativamente iguais. A 11ºC, a partir do 4º dia, as folhas soltas começaram a apresentar taxas de respiração superiores às das inteiras, chegando a ser o dobro nos 14º e 16º dias de armazenamento. O Q10 médio para as rúculas inteiras situou-se ao redor de 3,54 e para as folhas soltas, 5,74, na faixa de 1º a 11ºC. Não foi detectada produção de etileno. As embalagens para floretes de couves-flores tiveram pouca ou nenhuma influência em seu pH, acidez, ºBrix, luminosidade, croma, Hue e firmeza. A embalagem PD 941 foi a melhor por manter uma atmosfera modificada próxima à da recomendada para couves-flores (2-3% O2 e CO2 < 5%) e permitir menores perdas de vitamina C e melhores notas para vários atributos sensoriais de qualidade. Todas as embalagens mantiveram níveis de coliformes totais, bolores e leveduras bem abaixo dos limites permitidos pela Legislação Brasileira. As folhas de rúculas apresentaram níveis de coliformes totais ao redor de 10 5 UFC/g de produto no 10º dia a 5ºC, tornando-as impróprias para o consumo. Nestes 10 dias, as embalagens de PEBD e PP/PE se destacaram em relação às outras por reterem melhor a vitamina C e a coloração verde das folhas, por alcançarem melhores valores para sólidos solúveis, firmeza, presença de odor estranho, qualidade geral das folhas, deterioração e murchamento. As atmosferas modificadas desenvolvidas no interior destas embalagens, 5 a 7% O2 e 10 a 15% CO2, talvez sejam as mais indicadas para a conservação de rúculas minimamente processadas a 5ºC.
This work aimed at evaluating the effects of temperature on the metabolism of minimally processed and intact cauliflowers and salad rockets, and of different packaging materials on the quality of cauliflower florets and loose salad rocket minimally processed leaves. In the first experiment, intact cauliflowers, florets, intact salad rockets and loose salad rocket leaves were held at 1º, 5º and 11ºC and their respiration rates, ethylene production and temperature quotients (Q10) determined. In the second experiment, the florets were packed in low density polyethylene film (PEBD), co-extruded polyolefins (PD 941 and Clysar AFG) and in 12 and 20 mm polyvinyl chloride (PVC) overwrapping expanded polystyrene trays, and maintained at 5ºC/85-95% relative humidity for 14 days. In the third experiment, loose salad rocket leaves were held under the same conditions in bags of low density polyethylene film (PEBD), laminated polypropylene/polyethylene film (PP/PE), co-extruded polyolefins, PD 900 and Clysar HP and in expanded polystyrene trays overwrapped with 20 mm polyvinyl chloride (PVC). In the second and third experiments, physical, chemical, physical-chemical, microbiological and sensory analyses were performed. The respiration rates of both intact and fresh-cut cauliflowers declined over the 16-day period and were significantly the same at all temperatures studied. At 1º and 5ºC the respiration rates were the same but differed from those (cauliflower and florets) at 11ºC. From 1º to 11ºC, the Q10 for the cauliflowers was 2.2 and for the florets, 2.1. In contrast, the intact and fresh-cut salad rocket respiration rates were the same at 1º and 5ºC. At 11ºC, the fresh-cut salad rocket showed higher respiration rates than the intact ones as from the 4 th day. By the end of the experiment (14 th and 16 th days) the respiration rates of the fresh-cut salad rockets were twice those of the intact salad rockets, whose Q10 was about 3.45 from 1° to 11°C as compared to 5.74 for the fresh cut product. No ethylene production was detected by any sample at any temperature. The packaging materials had little or no effect on pH, titratable acidity, soluble solids, luminosity, chroma, hue and firmness of the florets at 5ºC. PD 941 seemed to be the best packaging for florets, maintaining the atmosphere closest to that recommended for intact cauliflower (2-3%O2, CO2 < 5%), showing the lowest vitamin C losses and the best scores for several of the quality attributes. In general, all the packaging materials kept the yeast and mold counts low and the total coliform counts far below the maximum values permitted by the Brazilian Legislation, during 14 days at 5ºC, except for fresh-cut salad rocket leaves, which presented 10 5 CFU/g total coliforms by the 10 th day, being inappropriate for consumption. For 10 day periods, PEBD and PP/PE were the best packaging materials for green color and vitamin C retention, higher soluble solids, firmness, lack of off-odors, overall quality, decay and shriveling. The modified atmosphere generated inside these packaging materials (5-7% O2, 10-15% CO2) seemed the best for maintaining the quality of fresh-cut salad rockets held at 5ºC.

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31

Nevhulaudzi, Thalukanyo. "Interactive effects of Bacillus subtilis and elevated temperature on germination, growth and grain quality of cowpea irrigated with acid mine drainage". Diss., 2019. http://hdl.handle.net/10500/26149.

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This study’s main goal was to evaluate Bacillus subtilis inoculation and mine water irrigation effect on germination, growth, nodulation, physiology and shoot/grain quality of cowpea genotypes exposed to extreme climatic conditions (elevated temperatures). The first experiment evaluated the interactive effect of Bacillus subtilis (BD233) inoculation and elevated temperature on germination indices and plumule lengths of three genotypes (Asetanapa, Soronko and Nyira) of cowpea. The results showed that interaction between B. subtilis (BD233) and temperature significantly (p<0.05) influenced the germination indices (germination percentage (G%), germination index (GI) and germination rate index (GRI)) and plumule length of cowpea seedlings and genotype responses were significantly different. At elevated temperature (35oC), inoculation with B. subtilis (BD233) enhanced seed germination and growth of cowpea. The second experiment evaluated the effect of temperature on growth and nutritional content of cowpea incubated for seven days in a growth chamber. The results showed that when cowpea genotype, Soronko, was incubated at different temperature regimes, the whole plant biomass, shoot carbon and crude protein contents were significantly affected with temperature increases at all three stages of the plants’ life cycle. The results suggest that the pre-flowering (40 DAP) and flowering (90 DAP) stages of cowpea compared to post-flowering (123 DAP) are more susceptible to elevated temperatures (30-35oC). The third experiment evaluated Bacillus subtilis inoculation and mine water irrigation effect on growth, nodulation, physiology and nutritional content of cowpea under glasshouse conditions. The results revealed that the interaction of B. subtilis (BD233) inoculation and mine water (75% AMD) irrigation was significant for the growth, nodulation, stomatal conductance, chlorophyll contents and shoot/grain nutritional quality of cowpea genotypes. In comparison with control, generally, B. subtilis inoculation enhanced the growth, nodulation and yield of all tested cowpea genotypes and irrigation with mine water significantly influenced the mineral contents in both shoot and grain of cowpea. Taken together, findings in this study have implications for cultivation of cowpea, an important candidate for food/nutrition security in Africa, under future climate change scenarios.
Environmental Sciences
M. Sc. (Environmental Sciences)

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32

Chandler, Robert Edward. "The effect of temperature and water activity on microbial growth rate and food spoilage". Thesis, 1988. https://eprints.utas.edu.au/18938/1/whole_ChandlerRobertEdward1988_thesis.pdf.

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The Square Root Model [✓r = b(T - T o )] was used to describe the temperature dependence of bacterial growth rate under conditions where temperature was the only limiting factor. It was validated for predicting the growth of the bacteria responsible for the spoilage of pasteurised, homogenised milk and for the in situ spoilage of the milk, over the storage range 0 to I5 ° C. A temperature function integrator, incorporating the Square Root Model and a To value of 263K was successfully used to monitor the temperature history of pasteurised, homogenised milk over a range of storage temperatures and to display the cumulated storage history at an arbitrary reference temperature (4 ° C). The spoilage rate of pasteurised homogenised milk, with respect to temperature, was described accurately by a Square Root Equation, possessing a To value similar to that of the psychrotrophic pseudomonads responsible for the spoilage of the milk. The Square Root Model described the temperature dependent variation in an induced bacterial lag phase, with the parameter, To being similar to that of the exponentially growing cell. The Square Root Model was shown to accurately predict bacterial growth under conditions where both temperature and water activity were limiting. Growth of the moderate halophile, Staphylococcus xylosus strain CM21/3, in media of different water activities, continued to be described by the Square Root Model, when either sodium chloride or glycerol was used as the humectant. The parameter To was constant, irrespective of water activity or the type of humectant used. A decreasing linear relationship was demonstrated between growth rate and decreasing water activity, with the minimum water activity for growth being dependant upon humectant used. This enabled the derivation of a modified Square Root Model, which was capable of describing the effect of both temperature and water activity on bacterial growth rate. The Square Root Model was validated for predicting the growth of the extreme halophiles, Halobacterium sp. strain HB9 and Halobacterium salinarium strain CM42/12, under conditions of varying water activity/salt concentration and temperature. The parameter To was constant irrespective of water activity. In addition, little change in growth rate, with change in water activity was noted.

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33

Ngomane, Dambuza. "Moderation of growth and sucrose flux in sugarcane by temperature". Thesis, 2005. http://hdl.handle.net/10413/4490.

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Sugarcane plants (cultivar NCo376) were studied to assess the effects of temperature and season (spring and autumn equinox) on the morphological and physiological response of stalk components. Plants were grown from single-eyed setts for ca. five months and then placed into three temperature controlled glasshouses (22/12°C (C), 27/17°C (W) and 32/22°C (H) day/night temperatures). The plants were sampled twice weekly over a one month period., and intemodes 4, 6 and 10 of the primary haulms of each plant sampled for growth and sugar analysis. During spring, the leaf emergence rates were 0.0303, 0.1095 and 0.1682 leaves d(-1) at temperatures C, W and H, respectively; and 0.0327, 0.0824 and 0.113 leaves d(-1) in autumn. The phyllochron intervals were 114°Cd in spring and 147°Cd in autumn. Highest green leaf blade area of the primary haulms was achieved at H (438.0 and 511.7 cm2 in spring and autumn, respectively). The stalk extension rates were 1.22, 1.02, 0.38 cm d(-1) (spring) and 1.35, 0.98, 0.45 cm d(-1) (autumn), respectively, in descending order of temperature. Total biomass and stalk biomass per plant were not affected by temperature, despite the differences in stem elongation. Internodes of plants at C were shorter but thicker and heavier than the comparable internodes of plants at W and H. In autumn, the mature internode sucrose concentrations were 35.5, 29.2 and 25.5% at C, W and H, respectively; corresponding to mean RS% of 5.7, 9.8 and 13.3%, and fibre % of 58.8, 61.1 and 61.3%, at the respective ascending order of temperature. Sucrose % in the mature internodes in spring were 27.8, 20.9 and 19.9% at C, W and H, respectively; corresponding to RS% of 5.9, 9.76 and 10.9% and fibre % of 66.3, 69.4 and 69.2% at the respective ascending order of temperature. Temperature effect on the concentration of the stalk components of the immature internodes was in general not significant. Sucrose partitioning coefficients in the mature internodes were 0.25, 0.21 and 0.20 in spring and 0.50, 0.32 and 0.21 in autumn (at C, W and H, respectively). Data that resulted from this study, which is isolated to temperature and cultivar NCo376 can be used in models of sugarcane that simulate leaf appearance and senescence, assimilate partitioning between leaf and stalk and assimilate partitioning between the stalk components namely sucrose, reducing sugars and fibre.
Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

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34

KILLI, HAWORTH DILEK. "The responses of crop and tree species to future elevated [CO2], temperature and drought stress". Doctoral thesis, 2017. http://hdl.handle.net/2158/1081327.

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Climate change offers particular challenges to sessile organisms such as plants that have to rely upon phenotypic adaptation to respond to higher temperatures, increased [CO2] and more frequent / severe drought. Climate change poses a severe challenge to food security via the maintenance of crop production and the sustainability of ecosystem services provided by urban trees and forests. This thesis has attempted to understand and address some of these challenges by studying the physiological, morphological and biochemical responses of different species / varieties of crops and Mediterranean urban trees to increased temperature, elevated [CO2] and drought. Indeed, the selection of crop varieties and urban tree species with desirable phenotypic responses to these conditions is a key component in efforts to mitigate the negative impact of climate change on agricultural services and urban environment.

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35

Khoo, Khar Yean. "Predictive modelling and experimental studies of thermal inactivation of bacteria as affected by combined temperature and pH in liquid". Thesis, 2006. http://hdl.handle.net/2440/37994.

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Continuous thermal pasteurisation of various bulk liquid media is an important step in the food and allied industries. The design of a continuous flow pasteuriser is typically predicated on mathematical models developed from experimental data - usually batch, bench - scale, ethods. Of particular interest is the effect of combined pasteuriser temperature ( T ) and liquid pH on inactivation and survivor of contaminants. However, bench - scale thermal survivor data may not adequately mirror those in a continuous flow pasteuriser. This research presents the development and experimental validation of rigorous models for thermal pasteurisation of bacteria as affected by combined process T - pH in both batch, bench - scale capillary studies ( static ) and in a pilot continuous flow pasteuriser ( dynamic ), within a defined liquid and range of exposure time, temperature and pH ( t - T - pH ). Five integrated stages in synthesis and model analysis were undertaken using stringent criteria for goodness of fit of an adequate model established. First, four published predictive models were assessed against published static data ( n [subscript T] = 248 ) for the thermal inactivation of Escherichia coli ( ATCC 25922 ) in a Carbopol ® 941 liquid food simulant in batch capillaries over a range of t - T - pH. The models tested were the Classical Arrhenius, Davey Linear - Arrhenius ( D - LA ), Square - Root ( Belehradek ) and a third - order Polynomial model ( nOP ). Analysis showed the D - LA model best satisfied the criteria for model selection and explained 96.0 % V in the thermal inactivation rate coefficient. Second, the D - LA model was assessed against limited, published dynamic data ( n [subscript T] = 109 ) for the same E. coli strain in identical food simulant. The model explained 60 % V in the thermal inactivation rate coefficient. On average, model predictions of survivor numbers from the dynamic data were less than that predicted from the static data, i.e. for a given ( t - T - pH ) more bacterial cells were apparently inactivated in the continuous flow pasteuriser than in bench - scale, batch capillary studies. Overall, however it was not clear from extensive analyses of available data whether there is a statistically significant difference in survivor numbers of viable E. coli between batch static and continuous flow dynamic data. Third, although the D - LA model best satisfied the criteria for goodness of fit of a model, it failed to accurately predict the observed tails in the static survivor data. New models ( KDT and a modified KDT ) were synthesised to predict tails and shoulders in survivor data. The modified KDT ( MKDT ) form gave improved predictive capability over the KDT model when assessed against published static survivor data for E. coli and L. monocytogenes ( n [subscript T] = 355 ) in the Carbopol food simulant. This model, however, could not be readily integrated with equations describing the performance of a continuous flow pasteuriser. Analyses indicated that a greater density of dynamic survivor data for E. coli was needed. Fourth, a pilot continuous flow pasteuriser was constructed and used to generate a greater density of dynamic survivor data of E. coli ( ATCC 25922 ) in a Carbopol ® 941 carrier liquid for rigorous comparison with predictions from the Lin ( 1976 ) isothermal continuous laminar flow process model. Direct steam injection heating was used. Extensive dye and digital - video studies, in a section of glass holding tube confirmed the practical implementation of the assumptions of laminar flow and rapid condensation of steam. Extensive practical experiments highlighted a non - isothermal condition along the holding tube. A highly linear dependence ( R ² > 0.90 ) of exposure temperature with holding tube length, i.e. exposure time, was demonstrated. This was accounted for using mathematical approaches and quantitatively incorporated into a D - LA model for the rate coefficient in an extended Lin process model. A block experimental design of 4 T ( 54, 56, 58, 60 ° C ) x 4 pH ( 4.5, 5.5, 6.5, 7.5 ) x 3 replicates with a total of ( n [subscript T] = 834 ) exposure times ( 16 - 198 s ) was carried out in the pilot continuous flow pasteuriser. Findings highlighted that greater numbers of E. coli were thermally inactivated in the flow pasteuriser than predicted. From a practical operating view, the predictions from the extended Lin model were therefore conservative - with reduced risk to public health. Highly significant differences in the rates of heat - up of bacteria in the pilot pasteuriser ( dynamic ) ( 0.0104 s ) compared with that in the batch ( static ) capillary tubes ( 1.6 s ) and, mode of heat transfer, together with partial effects of dispersion with increasing length of pasteuriser holding tube, are postulated to be the controlling process influences for the difference between the experimental survivor data and the extended Lin model predictions. The lack of agreement between the continuous pasteuriser data and predictions from the extended Lin model indicated that this model cannot be practically applied. A direct comparison of the experimentally derived dynamic survivor data from the pilot pasteuriser ( as ln N / N [subscript 0] ) was also made with both the published static and dynamic data at a number of defined t - T - pH. This comparison revealed that overall, more E. coli were inactivated in the pilot continuous flow pasteuriser than described by published batch static capillary and dynamic data. Importantly, these comparisons showed that batch thermal survivor data for E. coli do not adequately mirror those obtained in continuous flow systems. Fifth, in a search for an improved model for the inactivation data, the newly derived MKDT model was assessed against the experimental pilot pasteuriser data. This model was rejected, however, because it could not account satisfactorily for all tails in survivor curves. A Weibull form model with two coefficients ( a scale factor ( α ) and a shape factor ( β ) ) also did not adequately predict tailing and could not be reliably extrapolated with holding time. However, a modified Weibull form, also with two model coefficients ( β [subscript 0], β [subscript 1] ), did give an improved fit to available experimental data. This research highlighted statistically significant differences between the dynamic thermal survivor data for E. coli and standard bench - scale static capillary data for a defined liquid and range of t - T - pH. It is likely that findings from this study can be generalised. However, validation should be carried out for a range of common indicator micro - organisms in a range of liquid foods.
Thesis (Ph.D.)--School of Chemical Engineering, 2006.

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36

Khoo, Khar Yean. "Predictive modelling and experimental studies of thermal inactivation of bacteria as affected by combined temperature and pH in liquid". 2006. http://hdl.handle.net/2440/37994.

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Continuous thermal pasteurisation of various bulk liquid media is an important step in the food and allied industries. The design of a continuous flow pasteuriser is typically predicated on mathematical models developed from experimental data - usually batch, bench - scale, ethods. Of particular interest is the effect of combined pasteuriser temperature ( T ) and liquid pH on inactivation and survivor of contaminants. However, bench - scale thermal survivor data may not adequately mirror those in a continuous flow pasteuriser. This research presents the development and experimental validation of rigorous models for thermal pasteurisation of bacteria as affected by combined process T - pH in both batch, bench - scale capillary studies ( static ) and in a pilot continuous flow pasteuriser ( dynamic ), within a defined liquid and range of exposure time, temperature and pH ( t - T - pH ). Five integrated stages in synthesis and model analysis were undertaken using stringent criteria for goodness of fit of an adequate model established. First, four published predictive models were assessed against published static data ( n [subscript T] = 248 ) for the thermal inactivation of Escherichia coli ( ATCC 25922 ) in a Carbopol ® 941 liquid food simulant in batch capillaries over a range of t - T - pH. The models tested were the Classical Arrhenius, Davey Linear - Arrhenius ( D - LA ), Square - Root ( Belehradek ) and a third - order Polynomial model ( nOP ). Analysis showed the D - LA model best satisfied the criteria for model selection and explained 96.0 % V in the thermal inactivation rate coefficient. Second, the D - LA model was assessed against limited, published dynamic data ( n [subscript T] = 109 ) for the same E. coli strain in identical food simulant. The model explained 60 % V in the thermal inactivation rate coefficient. On average, model predictions of survivor numbers from the dynamic data were less than that predicted from the static data, i.e. for a given ( t - T - pH ) more bacterial cells were apparently inactivated in the continuous flow pasteuriser than in bench - scale, batch capillary studies. Overall, however it was not clear from extensive analyses of available data whether there is a statistically significant difference in survivor numbers of viable E. coli between batch static and continuous flow dynamic data. Third, although the D - LA model best satisfied the criteria for goodness of fit of a model, it failed to accurately predict the observed tails in the static survivor data. New models ( KDT and a modified KDT ) were synthesised to predict tails and shoulders in survivor data. The modified KDT ( MKDT ) form gave improved predictive capability over the KDT model when assessed against published static survivor data for E. coli and L. monocytogenes ( n [subscript T] = 355 ) in the Carbopol food simulant. This model, however, could not be readily integrated with equations describing the performance of a continuous flow pasteuriser. Analyses indicated that a greater density of dynamic survivor data for E. coli was needed. Fourth, a pilot continuous flow pasteuriser was constructed and used to generate a greater density of dynamic survivor data of E. coli ( ATCC 25922 ) in a Carbopol ® 941 carrier liquid for rigorous comparison with predictions from the Lin ( 1976 ) isothermal continuous laminar flow process model. Direct steam injection heating was used. Extensive dye and digital - video studies, in a section of glass holding tube confirmed the practical implementation of the assumptions of laminar flow and rapid condensation of steam. Extensive practical experiments highlighted a non - isothermal condition along the holding tube. A highly linear dependence ( R ² > 0.90 ) of exposure temperature with holding tube length, i.e. exposure time, was demonstrated. This was accounted for using mathematical approaches and quantitatively incorporated into a D - LA model for the rate coefficient in an extended Lin process model. A block experimental design of 4 T ( 54, 56, 58, 60 ° C ) x 4 pH ( 4.5, 5.5, 6.5, 7.5 ) x 3 replicates with a total of ( n [subscript T] = 834 ) exposure times ( 16 - 198 s ) was carried out in the pilot continuous flow pasteuriser. Findings highlighted that greater numbers of E. coli were thermally inactivated in the flow pasteuriser than predicted. From a practical operating view, the predictions from the extended Lin model were therefore conservative - with reduced risk to public health. Highly significant differences in the rates of heat - up of bacteria in the pilot pasteuriser ( dynamic ) ( 0.0104 s ) compared with that in the batch ( static ) capillary tubes ( 1.6 s ) and, mode of heat transfer, together with partial effects of dispersion with increasing length of pasteuriser holding tube, are postulated to be the controlling process influences for the difference between the experimental survivor data and the extended Lin model predictions. The lack of agreement between the continuous pasteuriser data and predictions from the extended Lin model indicated that this model cannot be practically applied. A direct comparison of the experimentally derived dynamic survivor data from the pilot pasteuriser ( as ln N / N [subscript 0] ) was also made with both the published static and dynamic data at a number of defined t - T - pH. This comparison revealed that overall, more E. coli were inactivated in the pilot continuous flow pasteuriser than described by published batch static capillary and dynamic data. Importantly, these comparisons showed that batch thermal survivor data for E. coli do not adequately mirror those obtained in continuous flow systems. Fifth, in a search for an improved model for the inactivation data, the newly derived MKDT model was assessed against the experimental pilot pasteuriser data. This model was rejected, however, because it could not account satisfactorily for all tails in survivor curves. A Weibull form model with two coefficients ( a scale factor ( α ) and a shape factor ( β ) ) also did not adequately predict tailing and could not be reliably extrapolated with holding time. However, a modified Weibull form, also with two model coefficients ( β [subscript 0], β [subscript 1] ), did give an improved fit to available experimental data. This research highlighted statistically significant differences between the dynamic thermal survivor data for E. coli and standard bench - scale static capillary data for a defined liquid and range of t - T - pH. It is likely that findings from this study can be generalised. However, validation should be carried out for a range of common indicator micro - organisms in a range of liquid foods.
Thesis (Ph.D.)--School of Chemical Engineering, 2006.

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37

Lin, Chi-An y 林祈安. "Effect of novel MAP on inhibiting microbial growth in Chinese ready-to-eat food products at ambient temperature". Thesis, 2001. http://ndltd.ncl.edu.tw/handle/80552050692442036355.

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碩士
輔仁大學
食品營養學系
89
Ready-to-eat products stored at ambient temperature (25oC or up) were studied in this study, and the inhibitory effect on microbial growth and the retention of quality by modified atmosphere packaging (MAP) were investigated. MAP model system was constructed to assess the inhibitory effect on microbial growth at ambient temperature, and the effectiveness associated with ethanol or limonene was also achieved. Subsequently, the modified atmosphere environment was applied to ready-to-eat food. More than 200 colonies were observed from the control stored at 37℃, 48hr in the model study; however, less microbial growth was noticed under 30-40% CO2 environment. MAP (CO2:O2:N2=30:5:65) incorporated with ethanol vapor functioned inhibiting E. coli growth, and the effectiveness related to the amount of ethanol vapor. Sushi packed under modified atmosphere containing 0.05% ethanol vapor was found no increase in cell number after 2 days, either at 18 or 25oC. Use of limonene associated with MA also performed inhibiting microbial growth; however the efficiency was not as good as ethanol vapor. In the study of noodle salad, cells were found increasing sharply in all packaged products at 25oC after 24hr. Apparently, MA incorporated with ethanol vapor is effective on inhibiting microbial growth in certain ready-to-eat food stored at ambient temperature, thus the extension of shelf life is achieved.

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38

MacPherson, Margaret Jeanette. "Effect of Salinity, Photoperiod, Temperature, and Restricted Food Intake on Growth and Incidence of Sexual Maturation of Labrador Arctic charr (Salvelinus alpinus)". 2012. http://hdl.handle.net/10222/15524.

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Economic viability of Fraser River, Labrador Arctic charr (Salvelinus alpinus) aquaculture in Atlantic Canada may be greatly improved if grow-out could be completed in seawater (30 ppt), while having a low incidence of sexual maturation before harvesting. Growth and survival in seawater was investigated among individually PIT-tagged Arctic charr reared in tanks in the laboratory. Direct transfer from freshwater to brackish water (20 ppt), and then acclimation to 30 ppt was successful. The manipulation of photoperiod, temperature, and food ration can be used as practical applications in aquaculture to arrest maturation; this was investigated in two additional experiments. The most effective photoperiod was LD18:6 for 6 weeks starting December 21, which reduced maturation to 43% compared to 78% in controls. Restricted ration from December 21 through March 15 had no effect on maturation, however, rearing females in 5°C compared to 10°C reduced maturation to 15% compared to >80% in controls.

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39

Kumari, Savitri. "Effect of Temperature and Storage on Free and Encapsulated Lactobacillus Acidophilus NCIM 2660 and Lactobacillus Bulgaricus NCIM 2056 in Different Food Matrix". Thesis, 2015. http://ethesis.nitrkl.ac.in/7269/1/2015_Effect_Kumari.pdf.

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In today’s world, the research into the health benefits of probiotics has rocketed sky high. The survivability of probiotics in foods depends on various factors during processing and storage. Heat is used in the process of a lot of foods. The fact was given that the amount of probiotics on the consumption of foods should be at least 107 CFU/g and that probiotic bacteria are sensitive to heat, so the survival of probiotics during thermal processing are the main challenges to food manufacturers. The main objective of the work is to study the effect of alginate encapsulation on temperature tolerance of Lactobacillus acidophilus NCIM 2660 and Lactobacillus bulgaricus NCIM 2056. Free cells of L. acidophilus NCIM 2660 and L. bulgaricus NCIM 2056 were exposed to different temperatures (60°C, 70°C, 80°C , and 90°C) at different intervals of time (1 min, 5 min, 10 min, 15 min, 20 min, 25 min, and 30 min). It was observed that L. acidophilus NCIM 2660 and L. bulgaricus NCIM 2056 can tolerate up to 100°C for 1 minute as free cells. In order to increase the temperature tolerance capacity, encapsulation was tried. Encapsulated L. acidophilus NCIM 2660 and L. bulgaricus NCIM 2056 were exposed to different temperatures i.e. >100°C for different time intervals. Results revealed that encapsulation could improve the temperature tolerance of L. bulgaricus NCIM 2056 up to 120°C for 1 minute. The present study revealed the advantage of encapsulation in protecting the bacterial cells from high temperature. These encapsulated cells can be utilized to formulate functional foods which will be heat processed before consumption. L. acidophilus NCIM 2660 and L. bulgaricus NCIM 2056 was incorporated in orange peel jelly as free cells and L. plantarum NCIM 2083 and L. bulgaricus NCIM 2056 was incorporated in dried snacks and their survivability in food matrix was studied for 3 weeks.

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40

Bally, Ian S. E. "The effect of preharvest nutrition and crop load on fruit quality and postharvest disease in mango (mangifera indica l.)". Thesis, 2007. http://era.daf.qld.gov.au/id/eprint/7956/.

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The aim of this thesis was to investigate how preharvest calcium (Ca) and nitrogen (N) nutrition, and low crop load during fruit development affect fruit quality and postharvest disease of Keitt mangoes (Mangifera indica L). Calcium, N and crop load were chosen because previous research and Gower reports implicate them as major factors in internal disorders, poor colour development, and postharvest disease in mangoes grown in the Mareeba district of Queensland. The symptoms and incidence of the internal fruit disorders seen in Keitt mangoes growing in Queensland were investigated to determine their similarity to other forms of internal disorders, and to develop a severity rating scale to assess fruit in later experiments. While the observed disorder in Keitt shared some symptoms with other named forms of disorders, not all symptoms were similar. The typical symptoms of patches of watery translucent flesh that breakdown and discharge cellular fluids in the more severe cases, are referred to as watery-pulp breakdown throughout this thesis. Watery-pulp breakdown-affected fruit had lower mesocarp Ca concentrations (0.05% dw) than healthy fruit (0.10% dw), but not N, K, P, and Mg concentrations. The severity of symptoms increased as maturity of the fruit increased, and early harvest may be a useful strategy to minimise the disorder. The first nutrition experiment investigated the effects of Ca, N and crop load on fruit quality in 6-year-old Keitt trees, between 1997 and 2000. Trees were fertilised with high (>1 kg Ca tree-1 and >300 g N tree-1) or nil concentrations of Ca and N, and natural or 70%-reduced crop loads. Fruit were assessed for watery-pulp breakdown, fruit colour, postharvest disease, fruit firmness, yield and average fruit weight. Although Ca was applied above the normal industry rates, there were no significant increases in fruit-Ca or leaf- Ca concentrations, or response in any of the fruit quality parameters measured. The factors influencing Ca concentrations in mesocarp were shown to be changes in dry matter accumulation and periodic reductions in Ca supply. Fruit from the high N treatment had significantly (p < 0.05) greater incidence and severity of postharvest side disease lesions (mainly anthracnose) but not stem lesions (mainly stem-end rot). The high N treatments also significantly (p < 0.05) reduced the percentage of yellow skin, and the percentage and intensity of red blush colour in ripe fruit. Watery-pulp breakdown, fruit firmness and average fruit weight were not significantly affected by the high N treatments. Reducing the crop load throughout fruit development significantly (p<0.05) increased the average fruit weights and the incidence of watery-pulp breakdown, but did not significantly affect the other fruit quality parameters measured. Further studies on Ca uptake and distribution were carried out in 3 additional experiments. The effects of Ca supply on fruit quality were investigated in a similar manner to the first experiment, by varying soil-applied Ca. Increased rates (1726 g tree-1 Ca) and more frequent applications (fortnightly) of gypsum were used to overcome leaching. Although soil Ca concentrations at harvest (0 - 30 cm deep) were increased by the Ca treatment, no significant effects were observed on fruit quality. The effect of leaf transpiration on leaf and fruit Ca concentrations was investigated by reducing leaf transpiration with an anti-transpirant throughout flowering and fruit development. Reduced leaf transpiration did not significantly affect mesocarp or leaf Ca, N, P, Mg, K, B concentrations, indicating the balance of transpiration mass flow between leaves and fruit is not a major factor in mango fruit Ca accumulation. Temporal changes in uptake and distribution of Ca during flowering and fruit development were studied using elemental strontium (Sr) as a Ca analogue. Sr and Ca concentrations were not similar during the experiment making prediction of Ca uptake and distribution based on Sr uptake and partitioning unsuitable. The effect of high N in increasing postharvest fruit disease in the first experiment was further studied to confirm the earlier results and to identify the mechanisms involved in this relationship. Fruit with a range of N concentrations were produced from trees fertilised with a range of N rates (0 417 g tree-1). The incidence and severity of postharvest anthracnose (C. gloeosporioides) increased as fruit-N increased, with significant relationships between anthracnose severity and exocarp-N concentration (anthracnose severity in sprung fruit = 163.18x2 151.85x + 36.987, r2 = 0.97**). High N had a similar effect on the incidence of Penicillium sp., Rhizopus sp., Guibnardia sp., or Epicoccum sp., but not Dothiorella sp. (stem-end rot). The antifungal compounds 5-n-pentadecyl resorcinol and 5-n-heptadecenyl resorcinol were influenced by exocarp-N in two ways. Resorcinol concentrations were lower in fruit with high exocarp-N than low-N fruit, and concentrations decreased faster in ripening fruit with high exocarp-N. The differences in resorcinol concentrations between high- and low-N fruit was strongest in the sprung stage of ripeness, and positive exponential relationships were found between exocarp-N and concentrations of 5-n-pentadecyl resorcinol (p=0.006. r2= 0.92) and 5-n-heptadecenyl resorcinol (p=0.016, r2=0.82). There were also positive relationships between anthracnose severity and 5-n-pentadecyl resorcinol (p=0.003, r2=0.86) and 5-n-heptadecenyl resorcinol (p=0.003, r2 =0.87). No significant differences were observed in exocarp morphology to explain the N effect on postharvest disease incidence or severity. The effect of timing of application of N on fruit quality and disease was investigated in a field experiment on 8-year-old Keitt mango trees on which N was applied at flowering, at mid fruit development, 2 weeks pre-harvest, or not applied (control). N applied at flowering and mid fruit development, significantly increased the severity of postharvest Colletotrichum sp. (p<0.05) Penicillium sp., Rhizopus sp., Guibnardia sp., and Epicoccum sp. (p<0.01) in ripe fruit. N applied 2 weeks prior to harvest did not significantly affect postharvest diseases, with fruit having similar anthracnose severity and incidence to the nil-N (control) treatment. Fruit Ca concentrations were not able to be manipulated by imposing field treatments. The inability to easily manipulate fruit Ca concentrations indicates it is unlikely to be an effective tool to manipulate fruit quality in mango. Nitrogen was shown to greatly influence fruit quality, having a major influence on postharvest disease, skin colour and sunburn susceptibility, but not watery-pulp breakdown. Increased N was also shown to reduce concentrations of natural antifungal resorcinol compounds as fruit ripened, making fruit more susceptible to postharvest rots. The sensitivity of fruit quality to N makes management of N a critical tool in managing fruit quality and disease susceptibility. Reduced crop load had a major effect on the incidence and severity of watery-pulp breakdown, indicating tree management practices to maximise crop load will also reduce the severity of watery-pulp breakdown.

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41

Beaupre, Steven John. "The effect of ambient temperature, food type, and meal size on post-feeding oxygen consumption and specific dynamic action in captive Dipsosaurus dorsalis". 1987. http://catalog.hathitrust.org/api/volumes/oclc/16919370.html.

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Thesis (M.S.)--University of Wisconsin--Madison, 1987.
Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 29-38).

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42

Yadav, Rina. "Effect of Encapsulation on Survival of Lactobacillus Fermentum NCIM 2156 and Lactobacillus Plantarum NCIM 2083 under Temperature Stress and Storage in Food Matrix". Thesis, 2015. http://ethesis.nitrkl.ac.in/7242/1/2015_Effect_Yadav.pdf.

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The objective of this study is to examine the temperature tolerance of bacterial cells and their viability after storage in the food matrix. Though probiotics are sensitive to temperature, encapsulation was used to protect the cells from various stress tolerance like temperature. For the present study Lactobacillus fermentum NCIM 2156 and Lactobacillus plantarum NCIM 2083 were selected. The bacterial cells were exposed at different temperature for different time intervals in water or oil bath, then their survivability was checked by spread plate method using MRS agar. The CFU/ml was calculated for Lactobacillus fermentum NCIM 2156 and Lactobacillus plantarum NCIM 2083 after incubation for 48 hrs at 37°C. It was observed that Lactobacillus fermentum NCIM 2156 and Lactobacillus plantarum NCIM 2083 could tolerate up to 90°C for 1 min and 100°C for 30 sec, respectively as free cells. Lactobacillus plantarum NCIM 2083 was encapsulated using alginate (3%) and the tolerance of encapsulated cells was studied. It was found that encapsulation could enhance the temperature tolerance of Lactobacillus plantarum NCIM 2083 up to 130°C for 2 min. Lactobacillus plantarum NCIM 2083 as free cells was incorporated in the orange peel jelly and their viability was checked. Synbiotic microcapsules were stored in health mix (Horlicks) for two weeks and the viability of cells in food matrix was checked after 2 weeks.

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43

Milham, Paul J., University of Western Sydney, College of Health and Science y Centre for Plant and Food Science. "The behaviour of cadmium in soil". 2008. http://handle.uws.edu.au:8081/1959.7/39519.

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Long-term low-level ingestion of cadmium (Cd) causes human health problems, and in Australia, vegetables supply ~40% of the Cd in the typical diet. Plants take up Cd from the soil; however, the uptake is poorly predicted by simple soil tests, such as the total concentration of Cd (Cdt). Therefore, a greater understanding of Cd behaviour in soils is needed to improve the prediction of Cd uptake by plants and open a new path to minimise the risks for human health. The objectives of the research in this thesis were to: identify key soil properties affecting Cd behaviour, identify/develop selective methods to measure them, and to formulate a conceptual model of Cd partitioning. These objectives were based on the hypothesis that empirical modelling informed by a better understanding of Cd chemistry would accurately describe Cd partitioning in soil. To test the hypothesis, the key properties were measured on soils from the peri-urban fringe of Greater Sydney (n = 41) and a series of models of increasing complexity were fitted to the data. A model with three explanatory variables— log10 Cdt, pH and log10 ECEC (effective cation exchange capacity)—explained 94.6% of variation in log10 CdCa (the concentration of Cd in solution in a suspension of soil in 10 mM CaCl2), which strongly supported the hypothesis. The study also indicated that the explanatory variables, Cdt, pH and ECEC, may describe Cd behaviour in many soils, and that for these general models, partition coefficients, such as log10 (Cdt/CdCa), are unsuitable dependent variables. The preceding model used Cdt as an explanatory variable, notwithstanding that labile Cd (CdE) was mechanistically preferable. However, CdE can only be measured using isotopic techniques: a requirement that has constrained the evaluation of CdE as an index of Cd behaviour and bioavailability. Therefore, a simple proxy measure of CdE was investigated. The literature indicated that solutions of chloride salts might selectively extract CdE, and Cd extracted into 1 M NH4Cl (CdNH4Cl) was compared with CdE measured by stable isotope dilution ICPMS. For 23 soils from the partitioning study, 1 M NH4Cl failed to completely extract CdE, unless the pH was less than 5. The cause(s) of this effect will be investigated with the aim of developing a universally applicable measure of CdE that does not require isotopic measurements. All models of Cd uptake by plants rely on soil properties measured on homogenised samples, although the distribution and bioavailability of Cd vary spatially in the field. Were such variability to increase at the micro-scale, its effects could erode the accuracy with which models could predict Cd behaviour and uptake. Consequently, I tested whether the distribution of Cd could be mapped by using synchrotron micro-x-ray fluorescence spectroscopy (micro- XRFS): the most sensitive method of observation. The soils examined contained 0.3–6.4 mg Cd/kg, i.e. were typical agricultural soils, and one was spiked to ~100 mg Cd/kg. Micro-XRFS mapped the Cd in the spiked soil, and in one particle in the other soils. For typical agricultural soils, the sensitivity realised in this study would have been sufficient to characterise the average Cd binding site, but fell at least 10-fold below that needed to map the Cd distribution in them. The research satisfied the objectives, advanced knowledge of Cd behaviour in soils, and provided new research leads. These leads include the possibility of developing general models of Cd partitioning in soils, derivatives of which may predict Cd uptake by plants. The accuracy of these models may be strengthened by the use of CdE as an explanatory variable, but may be weakened by the effects of in situ variation in the distribution of Cd. The benefits to human health of agricultural practices that decrease dietary Cd justify continuation of research to develop models that accurately predict Cd uptake by plants.
Doctor of Philosophy (PhD)

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Vilakazi, Busisiwe. "The effect of water stress and storage conditions on seed quality of chickpea genotypes characterized by differences in seed size and coat colour". Diss., 2018. http://hdl.handle.net/11602/1086.

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MSCAGR (Plant Production)
Department of Plant Production
Chickpea (Cicer arietinum L.) is an excellent utilizer of residual soil moisture in agricultural ecosystems. However, its seed quality and hence reproduction is constrained by water stress, seed size and storage conditions. This study was carried out at the University of KwaZulu- Natal (UKZN), Pietermaritzburg Campus. It was conducted to evaluate the performance of chickpea genotypes (Desi-K, Saina-K and ICCV-K) with different seed sizes on seedling emergence (i), seed ageing effect on seed quality and imbibition of genotypes produced under water stressed and non-stressed conditions (ii), and (iii) the effect of water stress during seed development on sugars and protein accumulation, germination and seed vigour. Pot experiments were conducted under glasshouse / tunnel conditions at the Controlled Environment Facilities (CEF). The experiment for objective 1 was laid out as a single factor in completely randomized design (CRD). Data on emergence rate, final hypocotyl and complete emergence was collected. The small seeded Desi-K showed higher and faster emergence compared to medium sized Saina-K and large seeded ICCV-K. In the experiment of the second objective, seeds of the three genotypes were first obtained by production under water stressed and non-stressed growing conditions. They were then aged for 0, 1, 3, 5, or 7 days at 41 ºC and 100% relative humidity to form a 2 x 3 x 5 (water levels x genotypes x ageing) factorial design. Data was collected on germination percentage (GP), mean germination time (MGT), electrical conductivity (EC), tetrazolium chloride test (TZ) and imbibition weight. Seed ageing caused progressive loss of seed viability and vigour in all genotypes, which resulted in lower GP, delayed MGT, reduced TZ staining, cell death and high solute leakage from the seeds produced under the two water regimes. However, the effect was more severe under water stressed conditions. In the experiment for objective 3, seeds of all three genotypes were larger when grown under non-stressed condition compared to those under water stressed condition. These larger seeds had higher seed viability and germination percentage but lower electrical conductivity and mean germination time. Stressed seeds had higher soluble sugars than non-stressed seeds. It was deduced that irrigation during seed development reduces the final sugars and protein content but increases the seed size and physiological quality parameters allied to production of chickpea. Therefore, water provision to chickpea crop is critical during seed development.
NRF

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45

Ryalls, James M. "The impacts of climate change and belowground herbivory on aphids via primary metabolites". Thesis, 2016. http://hdl.handle.net/1959.7/uws:37585.

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Global climate and atmospheric change (summarised as climate change for brevity) may alter patterns of crop damage by insect herbivores, but little is known about how multiple climate change factors, acting in tandem, shape such interactions. Crucially, the specific plant-mediated mechanisms underpinning these effects remain largely unknown. Moreover, research into the effects of climate change on leguminous plant species, which have the ability to fix atmospheric nitrogen (N2) via their association with root nodule-dwelling rhizobial bacteria, and their associated insect herbivores, is surprisingly scarce considering their increasing importance in terrestrial ecosystems worldwide. Using a model legume, lucerne, otherwise known as alfalfa, Medicago sativa (Fabaceae), and a model pest species, the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae), this work addresses how predicted changes in carbon dioxide (CO2) concentrations, temperature and rainfall patterns as well as interactions with other organisms, including the root-feeding weevil Sitona discoideus (Coleoptera: Curculionidae), might shape legume-feeding aphid populations in the future. Recent literature on the impacts of climate change on aphids and the biology and trophic interactions of lucerne aphids specifically were synthesised in chapters one and two, respectively. These chapters highlighted the importance of the interactions between multiple abiotic and biotic variables in shaping aphid population dynamics. Empirical research chapters three to six, using up to five lucerne genotypes (i.e. cultivars) in glasshouse and field experiments, addressed how A. pisum responded to the isolated and combined effects of climate change and root herbivory. In particular, chapter three determined the effects of elevated temperatures (eT) and elevated atmospheric CO2 concentrations (eCO2) on root-feeding S. discoideus larvae and their interaction with A. pisum. Chapter four addressed whether the effects of eT, eCO2 and simulated root damage on aphids could be explained by changes in plant amino acid concentrations. Chapter five built on the mechanistic findings from chapter four to determine whether specific groups of amino acids were responsible for driving the effects of eT and eCO2 on aphid fecundity, longevity and intrinsic rate of increase (rm). Chapter six extended this research to the field to determine the plant-mediated effects of water stress and root herbivory on aphids in a mixed grass–legume system. Lucerne demonstrated an over compensatory growth response to root herbivory by S. discoideus larvae by increasing net root biomass and nodulation by 31% and 45%, respectively. eT negated the positive effects of eCO2 on weevil larval development, as well as on a number of lucerne characteristics (e.g. nodulation and amino acid concentrations) and aphid performance parameters (e.g. population growth, fecundity and rm). Root herbivory by S. discoideus negatively impacted aphids in general, although effects were dependent on feeding duration and herbivore arrival sequence (i.e. whether aphids fed on the plant before or after root herbivory). While drought negatively impacted aphid abundance, potentially via reduced phloem turgor and sap viscosity, the effects of eT, eCO2 and root herbivory on aphids were often driven by concentrations of specific amino acid groups. Nitrogen (N) leached from lacerated lucerne root nodules by S. discoideus led to increased concentrations of N in a neighbouring grass, Phalaris aquatica (Poaceae), with knock-on effects on plant competition and community dynamics. The opposing effects of eT and eCO2 on plant characteristics and both aboveground and belowground herbivores demonstrates the importance of combining trophic complexity with multiple climatic factors as a means of gaining realistic insights into how insect and plant communities will respond under future conditions. Identifying the specific amino acid changes underpinning aphid responses to climate change and root herbivory offers the potential for breeding aphid resistance traits into lucerne cultivars and informing adaptation strategies against future threats. Changes in precipitation patterns and plant-mediated indirect aboveground–belowground herbivore interactions can alter the outcome of competition between N-fixing legumes and non-N-fixing grasses, with important implications for plant community structure and productivity. Avenues for future research are explored and other causal agents of changes in aphid performance are discussed, which may further elucidate the mechanisms underpinning climate change and belowground herbivory impacts on aphid pests.

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46

Losee, James P. "Trophically transmitted parasites as ecosystem indicators : relationships among parasite community structure, juvenile salmon diet composition, and ocean conditions". Thesis, 2012. http://hdl.handle.net/1957/29897.

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Recent research conducted throughout the Northern California Current (NCC) on the ecology of Pacific salmon (Oncorhynchus spp.) indicates that variable ocean conditions affect the community composition of zooplankton in the nearshore environment which, in turn, can affect the quality of prey for fish, sea birds and mammals. Interannual variability in the quality and composition of the copepod community in the NCC during early marine residency of some Pacific salmon populations is related to survival to adulthood. However, copepods make up a small portion of the diet of coho and Chinook salmon, and the mechanistic linkages between ocean climate, zooplankton composition and salmon prey remain unclear. Parasite analysis provides a supplement to traditional diet analysis that can describe the foraging history of a host species. Coho salmon (O. kisutch) and Chinook salmon (O. tshawytscha) serve as hosts to an array of marine parasites acquired through consumption of infected intermediate hosts such as copepods, euphausiids, and planktivorous fishes. Causing little or no harm to their salmon host, the presence of trophically transmitted parasites provides information on the dietary history of their salmonid host beyond the 24 hours associated with traditional diet analysis. This study (1) examined differences in feeding behavior of coho and Chinook salmon during their early marine residency using both stomach and parasite community analyses and (2) tested the hypothesis that variability in ocean circulation patterns (measured through the Pacific Decadal Oscillation, sea surface temperature (SST) and Bakun's upwelling index) and copepod species composition are related to variability in the community structure of trophically transmitted marine parasites found in juvenile salmon. I compared the abundance and species composition of parasites recovered from juvenile Columbia River coho and upper Columbia River summer and fall Chinook salmon captured off the coast of Washington from 2002 to 2009. I also compared interannual variability in parasite assemblages to physical and biological indices of ocean conditions. Coho and Chinook salmon consumed similar prey taxa; however, the species richness and abundance of trophically transmitted parasites indicated that Chinook salmon consumed a greater diversity and abundance of infected prey. In addition, differences in the abundance of fish in the diet and Anisakis simplex, a parasitic nematode known to infect salmon through fish consumption, suggest that Chinook salmon consistently consumed more fish prey than coho. In contrast, coho appeared to consume more euphausiids as indicated by stomach content analysis and increased abundance of the euphausiid parasite, Rhadinorhynchus trachuri. Shifts in the parasite community composition of both coho and Chinook salmon were related to interannual variability in SST and the biomass of southern-origin copepods (r > 0.7, P < 0.05). The acanthocephalan R. trachuri and a tetraphyllid cestode were associated with "warm" SSTs and greater biomass of lipid-poor, subtropical copepods while the nematode A. simplex was more abundant in years of "cold" SST and a relatively low biomass of subtropical copepods. These results provide novel insight into differences in the diet of Columbia River coho and Chinook salmon and illustrate linkages between ocean climate, zooplankton community composition and salmon diet during early marine residency.
Graduation date: 2012

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