Tesis sobre el tema "Domain Studies"
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Batra, Sharat. "Magneto-optical studies of domain wall oscillations /". The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487265555439935.
Texto completoParnas, Henrietta. "Binding studies of the SH3D domain of intersectin". Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=87005.
Texto completoLa protéine structurale intersectine1 est impliquée dans l'endocytose, l'apoptose, la transduction de signaux ainsi que la régulation des réarrangements du cytosquelette. La compréhension des interactions d'intersectine1 avec d'autres protéines a des conséquences importantes pour la biologie cellulaire. L'activité de la protéine activatrice de la GTPase Cdc42 (CdGAP) est empêchée par l'intersectine1 ce qui augmente la polymérisation des d'actine. La région centrale basique de CdGAP qui contrôle la liaison de la protéine avec ses substrats ne contient aucun motif qui se lie aux domaines SH3. Quelques peptides de de cette région se lient à intersectine1 SH3D en utilisant un criblage d'une banque de peptides, et ont été proposés de contenir des motifs SH3 nouveaux. La liaison avec un peptide entraîne des perturbations de déplacements chimiques dans le spectre de résonance magnétique nucléaire (RMN) d'une protéine. Dans cette recherche, la RMN fut utilisée pour étudier l'interaction du domaine SH3D de l'intersectine1 avec des peptides de quelques associés putatifs. Les peptides de CdGAP qui furent identifiés lors du criblage d'une banque de peptides ne se lient pas à l'intersectine SH3D recombinante. Deux peptides de la protéine dynamine qui contiennent des motifs SH3 typiques ne se lient pas avec l'intersectine1 SH3D, mais un plus long peptide de synaptojanine, riche en proline, se lie faiblement. Un peptide de CdGAP, identifiée par homologie avec ce peptide, s'est aussi lié. Les changements de déplacements chimiques furent utilisées pour déterminer le site de liaison sur la structure de l'intersectine2 SH3D. Les sites de liaison des deux peptides se superposent. Cette liaison se produit avec des acides aminés qui sont conservés parmi les domaines SH3. Ceci est le premier peptide de CdGAP qui se lie à l'intersectine1 SH3D. Son homologie au peptide de synaptojanine peut aider la caractérisation de ce motif de liai
Menderin, Nathan. "Studies on the Human Sp1 DNA-Binding Domain". Thesis, University of Exeter, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.507135.
Texto completoHardman, C. H. "NMR studies of the A domain of HMG1". Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603702.
Texto completoEdwards, Simon David. "Structural studies of the FERM domain of moesin". Thesis, Birkbeck (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268755.
Texto completoBerthoumieu, Olivia. "Single molecule studies of seven transmembrane domain proteins". Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:ff7ae71d-5481-4523-812b-2128fe32f5fc.
Texto completoWilliams, Samantha Catherine. "Studies of the domain structure of complement factor B". Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358907.
Texto completoAli, M. "Structural studies of Xenopus laevis Quaking protein QUA1 domain". Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595447.
Texto completoPommer, Ansgar J. "Mechanistic studies on the DNase domain of colicin E9". Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361425.
Texto completoScott, Nerida Robyn. "Folding studies of the glucocorticoid receptor DNA-binding domain". Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627279.
Texto completoThompson, Elizabeth Claire. "Studies on SET and MYND domain proteins in Drosophila". Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612456.
Texto completoLeung, Adelaine Kwun-Wai. "Structural studies of the spliceosomal U4 snRNP core domain". Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614942.
Texto completoTran, Huong Jade Thien Thi. "Structural and functional studies of JAB1/MPN domain proteins". Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614104.
Texto completoPurves, Maud. "The D-domain of fibrin : structural and functional studies". Doctoral thesis, University of Cape Town, 1987. http://hdl.handle.net/11427/27202.
Texto completoWu, Dimao. "Synthetic Studies on Phorboxazole Tail Domain and Amphidinolide C". The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354560325.
Texto completoSchmiedeskamp, Mia Ruth. "NMR studies of the DNA-binding domain of ADR1 /". Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/9208.
Texto completoPatterson, C. J. F. "Topographic, ultrasonic and diffraction studies of helical antiferromagnets". Thesis, University of Hull, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381882.
Texto completoPowell, Ryan R. "Outback Nevada| Public Domain and Environmental Challenge". Thesis, University of Nevada, Reno, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10633860.
Texto completoWith the arrival of Euro-Americans to Nevada, settlers and travelers experienced struggles and opportunities on Nevada’s marginal lands. These lands did not fit well with Euro-American ideas of progress and resource-use throughout the second part of the nineteenth century. After 1848, these marginal lands became part of America’s public domain with little promise for permanent settlements. Between 1860 and 1905, Euro-Americans imposed unsustainable land-uses on Nevada’s marginal lands. Due to increased competition on limited rangelands, federal land managers working for the United States Forest Service (USFS) came to Nevada after 1905 and secured the water resources in the highest mountains to promote favorable conditions of water flows for preferred local settlers. These settlers were the cattle ranchers with permanent home ranches that depended on water from the high mountains for summer grazing and haymaking. In the early twentieth century, beginning with the creation of the USFS in 1905 and ending with the Taylor Grazing Act in 1934, federal land managers were critical to maintaining successful settlements on a challenging environment in outback Nevada.
Zhang, Min. "Crystallographic studies of the E. coli puta proline dehydrogenase domain /". free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p1426119.
Texto completoWalke, Stefan. "Biochemical and structural studies of the spliceosomal snRNP core domain". Thesis, University of Cambridge, 2000. https://www.repository.cam.ac.uk/handle/1810/251736.
Texto completoPatel, Pryank. "Structural studies of the inner DysF domain of human myoferlin". Thesis, Birkbeck (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479383.
Texto completoAntoniadou, Eleni. "Studies on the biochemistry of the targetin domain of Lysostaphin". Thesis, University of Nottingham, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411404.
Texto completoJones, Gareth. "NMR studies of the DNA-binding domain of B-Myb". Thesis, University of Kent, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270703.
Texto completoMeng, Ling Guang. "Frequency domain methods for turbogenerator and power system transient studies". Thesis, University of Newcastle Upon Tyne, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308982.
Texto completoWatson, Martin. "Structural studies on the PX domain of sorting nexin 1". Thesis, University of Sheffield, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.434574.
Texto completoJoo, Chulmin 1976. "Spectral-domain optical coherence phase microscopy for quantitative biological studies". Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/43142.
Texto completoIncludes bibliographical references.
Conventional phase-contrast and differential interference contrast microscopy produce high contrast images of transparent specimens such as cells. However, they do not provide quantitative information or do not have enough sensitivity to detect nanometerlevel structural alterations. We have developed spectral-domain optical coherence phase microscopy (SD-OCPM) for highly sensitive quantitative phase imaging in 3D. This technique employs common-path spectral-domain optical coherence reflectometry to produce depth-resolved reflectance and quantitative phase images with high phase stability. The phase sensitivity of SD-OCPM was measured as nanometer-level for cellular specimens, demonstrating the capability for detecting small structural variation within the specimens. We applied SD-OCPM to the studies of intracellular dynamics in living cells and the detection of molecular interactions on activated surfaces as a sensor application.In the study of intracellular dynamics, we measured fluctuation of localized field-based dynamic light scattering within cellular specimens. With its high sensitivity to amplitude and phase fluctuations, SD-OCPM could observe the existence of two different regimes in intracellular dynamics. We also investigated the effect of an anti-cancer drug, Colchicine and ATP-depletion on the intracellular dynamics of human ovarian cancer cells, and observed the modification in diffusion characteristics inside the cells. Based on the optical sectioning capability of SD-OCPM, quantitative phase imaging was performed to examine slow dynamics of living cells. Through time-lapsed imaging and spectral analysis on the dynamics at the vicinity of cell membrane, we observed the existence of dynamic and independent sub-domains inside the cells that fluctuate at various dominant frequencies with different frequency contents and magnitudes.SD-OCPM was further utilized to measure molecular interactions on activated sensor surfaces.
(cont) The method is based on the fact that phase varies as analyte molecules bind to the immobilized probe molecules at the sensing surface and SD-OCPM can measure small phase alteration at any surface with high sensitivity. We have measured a -1.3 nm increase in optical thickness due to the binding of streptavidin on a biotin-activated substrate in a micro-fluidic device. Moreover, SD-OCPM was extended to image protein array chips, demonstrating its potential as a multiplexed protein array scanner.
by Chulmin Joo.
Ph.D.
Suckling, Richard John. "Structural and functional studies of [delta]-COP Mu-homology domain". Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607739.
Texto completoWang, Wooi Koon. "Biophysical and functional studies of SAM Domain of human p73α". Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621141.
Texto completoMorgan, Hugh P. "Structural and biochemical studies of cold shock domain containing proteins". Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/15432.
Texto completoSekharan, Monica R. "Structural studies of the cGMP-binding GAF domain of PDE5A /". Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/8502.
Texto completoIto, Yoshimichi. "Frequency Domain Studies on Sampled-Data Systems Using FR-Operators". 京都大学 (Kyoto University), 2011. http://hdl.handle.net/2433/142255.
Texto completoBenetti, Federico. "Structural studies on the C-terminal domain of human PMCA1b". Doctoral thesis, Università degli studi di Padova, 2008. http://hdl.handle.net/11577/3425143.
Texto completoNanao, Max H. "Structural and computational studies of the potassium channel T1 domain and the ligand binding domain of a glutamate receptor /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3036994.
Texto completoLu, Zhisheng. "Structural studies of HIV-1 Vif and its SOCS-box domain". Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/structural-studies-of-hiv1-vif-and-its-socsbox-domain(710df582-c838-4935-b6f6-c023195997c7).html.
Texto completoPlans, Calafell Vanessa. "Studies on RNF8, a ubiquitin ligase with a RING finger domain". Doctoral thesis, Universitat de Barcelona, 2005. http://hdl.handle.net/10803/1868.
Texto completoL'heterodimer format per UBC13-UEV te activitat de conjugació de ubiquitines mitjançant enllaços que utilitzen la lisina en posició 63 de la ubiquitina en contes de la lisina en posició 48. Aquesta modificació no es reconeguda pel proteasoma i per tant no implica una degradació de la proteïna que la porta. La cerca de proteïnes que interactuen amb UBC13 ens va permetre de identificar dues proteines: RNF8 i KIAA0675 que contenen un domini RING finger, pel qual interactuen amb UBC13. RNF8 te activitat autocatalítica de lligasa de ubiquitines, el que li permet elongar cadenes de poliubiquitines tant per lisina 48 com per lisina 63; aquesta darrera depèn de l'activitat catalítica de UBC13. A més, RNF8 co-localitza amb UBC13 en estructures nuclears.
L'estructura en dominis d'RNF8 és la mateixa que la del regulador del checkpoint mitòtic CHFR, ambdues proteïnes tenen un domini RING finger de reclutament d'enzims de conjugació de ubiquitines, al extrem amino terminal i un domini FHA de reconeixement de fosfopèptids. RNF8 localitza en el nucli cel·lular i te un patró d'expressió depenent de cicle amb uns nivells proteics que augmenten progressivament de G1 a M, assolint un màxim en metafase seguit d'una brusca desaparició en anafase i una recuperació de l'expressió de la proteïna en fases més tardanes de la mitosi. La sobre-expressió de RNF8 causa una reducció de la població en G2-M i una acumulació de cèl·lules en G1. La depleció d'RNF8 mitjançant la tècnica de RNAi no causa cap efecte significatiu sobre el cicle cel·lular basal. En canvi, la depleció d'RNF8 causa un retard en la sortida de mitosi després d'un arrest induït per nocodazole, fet que s'associa amb una reducció del turnover de la ciclina B1, que és un substrat de l'APC/C. Recíprocament, la sobre-expressió de RNF8 impedeix a les cèl·lules d'acumular-se en G2-M en resposta a un tractament amb nocodazole. A més l'activació del Spindle Assembly Checkpoint (SAC), mitjançant un breu tractament amb nocodazole, en combinació amb la sobre-expressió d'RNF8 impedeix que la proteïna del checkpoint Mad2 es localitzi als quinetocors. Aquesta funció d'RNF8 depèn de l'activitat lligasa ja que un mutant d'RNF8 incapaç de conjugar ubiquitines produeix uns efectes significativament atenuats en comparació amb la proteïna salvatge. Aquestes observacions suggereixen que RNF8 és un regulador negatiu del SAC i permet la reactivació de l'APC/C en condicions d'estrès mitòtic.
A més, RNF8 presenta activitat pro-apoptòtica. L'expressió ectòpica d'RNF8 en cèl·lules HeLa activa les caspases-3 i -8 i l'activitat pro-apoptòtica resultant es pot inhibir mitjançant la incubació amb els inhibidors de caspases ZVAD i ZDEVD. La mort cel·lular induïda per RNF8 es veu atenuada en gran mesura per la sobre-expressió del mutant d'RNF8 que no te activitat lligasa de ubiquitines. El tractament amb diferents agents genotòxics provoca una acumulació dosi-depenent de la proteïna RNF8 endògena que no es correlaciona amb un increment dels nivells de transcrit. Finalment, la depleció d'RNF8 permet a les cèl·lules HeLa de ser més resistents al tractament amb etopòsid, suggerint que RNF8 te un paper important regulant la mort cel·lular causada per aquesta droga.
Ubiquitylation is a post-translational protein modification, which regulates very different signaling pathways and biological processes. Ubiquitylation occurs thanks to the catalytic activity of ubiquitin activating enzymes or E1, ubiquitin conjugating enzymes or E2 and ubiquitin ligases or E3 and the substrate can be modified with one or more ubiquitin moieties that form polyubiquitin chains.
The heterodimeric ubiquitin conjugating enzyme (E2) UBC13-UEV mediates polyubiquitylation through lysine 63 of ubiquitin (K63), rather than lysine 48 (K48). This modification does not target proteins for proteasome-dependent degradation. Searching for potential regulators of this variant polyubiquitylation we have identified two proteins, namely RNF8, KIA00675, that interact with UBC13 through their RING finger domains. These domains can recruit, in addition to UBC13, other E2's that mediate canonical (K48) polyubiquitylation. RNF8 has ubiquitin ligase autocatalytic activity that elongates chains through either K48 or K63 of ubiquitin, and UBC13 activity is required to elongate the latter chains. RNF8 and UBC13 co-localize in the nucleus.
RNF8 is a ubiquitin ligase that bears a FHA domain near its amino end, and a RING finger domain at its carboxy terminus, through which it can recruit several ubiquitin-conjugating enzymes. In metazoans, only the mitotic checkpoint regulator CHFR shares this domain architecture. RNF8 follows a cell-cycle-dependent turnover, with levels increasing from G1 to M, reaching a maximum at metaphase, followed by an abrupt decline at anaphase, and recovery of protein levels at the end of mitosis. Overexpression of RNF8 caused a reduction of the G2-M population and an accumulation of cells in G1, while siRNA depletion did not significantly alter the basal cell cycle. Depletion of RNF8 caused a delay in the exit from nocodazole-induced arrest, which was associated with a reduced turnover of the APC/C substrate cyclin B1. Conversely, cells overexpressing RNF8 failed to arrest at G2-M upon treatment with nocodazole. In addition, activation of the spindle assembly checkpoint with a brief exposure to nocodazole in combination with RNF8 overexpression prevented the localization of Mad2 to kinetochores. These functions of RNF8 were dependent on its ubiquitin ligase activity, since a ligase-inactive mutant produced significantly attenuated effects as compared to the wild-type protein. Taken together, these observations suggest that RNF8 is a negative regulator of the spindle assembly checkpoint that reactivates the APC/C under conditions of mitotic stress.
In addition,RNF8 functions as a proapoptotic protein. Ectopic expression of RNF8 in HeLa cells induced the activation of caspases 3 and 8, and the ensuing apoptosis was prevented by incubation with the caspase inhibitors ZVAD and ZDEVD. The apoptotic death induced by RNF8 was greatly attenuated by introduction of a point mutation in its RING finger domain that rendered it non-functional as a ubiquitin ligase, indicating that this function is essential for the proapoptotic activity of RNF8. Treatment with DNA-damaging agents causes a dose-dependent accumulation of endogenous RNF8 without increasing its mRNA levels. Finally, depletion of RNF8 with specific siRNA duplexes effectively prevented the apoptosis and caspase-3 up regulation induced by the topoisomerase II inhibitor etoposide, suggesting that RNF8 plays a major role in regulating apoptotic death caused by DNA damaging agent.
Salek, Reza M. "NMR studies of the heat shock protein 90 N-terminal domain". Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1446807/.
Texto completoMarigheto, Niusa A. "Time-Domain NMR Studies of the Internal Quality of Horticultural Products". Thesis, University of East Anglia, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485289.
Texto completoHart, Christopher T. "Functional and Structural Studies of the Anti- MinCD Domain of MinE". Thesis, University of Ottawa (Canada), 2010. http://hdl.handle.net/10393/28643.
Texto completoCheng, Shan Amy. "Structure-function studies of secreted PDZ domain-containing protein 2 (sPDZD2)". Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558101.
Texto completoDutnall, Robert Nicholas. "Structural and functional studies of a zinc finger DNA-binding domain". Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360030.
Texto completoWilson, Gillian Mary. "High frequency dielectric studies of cell suspensions using time domain reflectometry". Thesis, Bangor University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305946.
Texto completoFowler, S. B. "Folding studies on an immunoglobulin domain using chemical and mechanical methods". Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599147.
Texto completoFord, M. G. J. "Structural and functional studies on ANTH and EUTH domain-containing proteins". Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599114.
Texto completo鄭珊 y Shan Amy Cheng. "Structure-function studies of secreted PDZ domain-containing protein 2(sPDZD2)". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39558101.
Texto completoJacks, Amanda Jane. "NMR studies of the starch-binding domain of Aspergillus niger glucoamylase". Thesis, University of Sheffield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364191.
Texto completoMcIntosh, Pauline Bernadette. "NMR based studies of the DNA-binding domain from B-Myb". Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388299.
Texto completoKlemm, Juli Dawn. "Structural and biochemical studies of Oct-1 POU domain-DNA interactions". Thesis, Massachusetts Institute of Technology, 1995. http://hdl.handle.net/1721.1/32667.
Texto completoShu, Ningcheng. "Structural studies of biotinyl domain of E. coli acetyl-CoA carboxylase". Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620559.
Texto completoMcIntosh, Lisa. "Development and characterisation studies of a type III fibronectin domain pair". Thesis, University of Strathclyde, 2014. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=24607.
Texto completoHanson, Sonya M. "Structural, biochemical and computational studies of TRP channel transmembrane domain modularity". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:328269a9-11c0-4d5b-9cb7-d7433cf4d6c4.
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