Literatura académica sobre el tema "Direct nucleic acid detection"
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Artículos de revistas sobre el tema "Direct nucleic acid detection"
Zezza, Paola, María Isabel Lucío, Estrella Fernández, Ángel Maquieira y María-José Bañuls. "Surface Micro-Patterned Biofunctionalized Hydrogel for Direct Nucleic Acid Hybridization Detection". Biosensors 13, n.º 3 (23 de febrero de 2023): 312. http://dx.doi.org/10.3390/bios13030312.
Texto completoOuyang, Wei y Jongyoon Han. "Universal amplification-free molecular diagnostics by billion-fold hierarchical nanofluidic concentration". Proceedings of the National Academy of Sciences 116, n.º 33 (29 de julio de 2019): 16240–49. http://dx.doi.org/10.1073/pnas.1904513116.
Texto completoIwanaga, Masanobu. "High-Sensitivity High-Throughput Detection of Nucleic Acid Targets on Metasurface Fluorescence Biosensors". Biosensors 11, n.º 2 (27 de enero de 2021): 33. http://dx.doi.org/10.3390/bios11020033.
Texto completoKnight, Ivor T., Jocelyne DiRuggiero y Rita R. Colwell. "Direct Detection of Enteropathogenic Bacteria in Estuarine Water Using Nucleic Acid Probes". Water Science and Technology 24, n.º 2 (1 de julio de 1991): 261–66. http://dx.doi.org/10.2166/wst.1991.0070.
Texto completoKricka, Larry J. y Paolo Fortina. "Analytical Ancestry: “Firsts” in Fluorescent Labeling of Nucleosides, Nucleotides, and Nucleic Acids". Clinical Chemistry 55, n.º 4 (1 de abril de 2009): 670–83. http://dx.doi.org/10.1373/clinchem.2008.116152.
Texto completoUno, Takeshi, Toshihito Ohtake, Hitoshi Tabata y Tomoji Kawai. "Direct Deoxyribonucleic Acid Detection Using Ion-Sensitive Field-Effect Transistors Based on Peptide Nucleic Acid". Japanese Journal of Applied Physics 43, No. 12B (19 de noviembre de 2004): L1584—L1587. http://dx.doi.org/10.1143/jjap.43.l1584.
Texto completoFaron, Matthew L., Nathan A. Ledeboer, Jessica Connolly, Paul A. Granato, Brenda R. Alkins, Jennifer Dien Bard, Judy A. Daly, Stephen Young y Blake W. Buchan. "Clinical Evaluation and Cost Analysis of Great Basin Shiga Toxin Direct Molecular Assay for Detection of Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Specimens". Journal of Clinical Microbiology 55, n.º 2 (7 de diciembre de 2016): 519–25. http://dx.doi.org/10.1128/jcm.01939-16.
Texto completoJi, Minghui, Yun Xia, Jacky Fong-Chuen Loo, Lang Li, Ho-Pui Ho, Jianan He y Dayong Gu. "Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform". RSC Advances 10, n.º 56 (2020): 34088–98. http://dx.doi.org/10.1039/d0ra04507a.
Texto completoBaron, Ellen Jo, Fred C. Tenover y Devasena Gnanashanmugam. "Direct Detection of Mycobacterium tuberculosis in Clinical Specimens Using Nucleic Acid Amplification Tests". Clinical Microbiology Newsletter 40, n.º 13 (julio de 2018): 107–12. http://dx.doi.org/10.1016/j.clinmicnews.2018.06.003.
Texto completoZhou, Yunying, Fengyan Pei, Mingyu Ji, Li Wang, Huailong Zhao, Huanjie Li, Weihua Yang, Qingxi Wang, Qianqian Zhao y Yunshan Wang. "Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative". PLOS ONE 15, n.º 11 (18 de noviembre de 2020): e0241469. http://dx.doi.org/10.1371/journal.pone.0241469.
Texto completoTesis sobre el tema "Direct nucleic acid detection"
Lores, Lareo Pablo. "Nucleic acids and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry". Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/20133.
Texto completoThe field of nucleic acid detection has evolved swiftly in recent years. From quantification of micro RNA for the study of cell death, proliferation, and regulation, to the assessment of the influence of genetic variability towards disease development and treatment, the analysis of nucleic acids will play a central role in future medicine. In that regard, the detection of SNPs, as the primary source of genetic variability and the most challenging mutation from the analytical point of view, will be at the forefront of the discussion. Methods for the detection of SNPs not only require sensitivity, selectivity and robustness, but they should also allow multiplexing and offer high throughput in order to face the growing analysis demand In this work an assay for the detection of nucleic acids and single nucleotide polymorphisms (SNPs) was developed. The reaction system for the detection of nucleic acids is based on the interaction between two modified peptide nucleic acid (PNA) oligonucleotides. The first incorporated a C-terminal thioester (donor probe), and the second one a N-terminal cysteinyl residue (acceptor probe). In addition, the donor probe is functionalized with a metal-tag, which consist of a macrocyclic metal chelate complex of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) with a chelated lanthanoide. A biotin tag for purification by streptavidin magnetic particles was incorporated in the acceptor probe. The target DNA strand brings together the reporter probes allowing the chemical reaction. The resulting ligation product contains the metal-tag and the biotin, which is used to purify the product before measurement in the ICP-MS system. The lanthanoid concentration is used as an indicator of the ligation product, which at the same time serves as reporter of the target template. The methodological limit of detection achieved with this system was 29 pM with RSD of 6.8% at 50 pM (n=5). Detection of SNPs was performed using a combination of two sets of PNA probes labeled with different lanthanoid metal tags. The first probe set targeted the sequence where the SNP was present (reporter probe system), while the second set of probes was designed to bind to a neighboring sequence (control probe system). The signals of both lanthanides were used to establish a ratio that allowed the detection of the SNP. This assay was successfully used to simultaneously differentiate between alleles of 3 SNPs by measuring six lanthanoids at 5 nM concentration.
Chatwell, Nicola. "Nucleic acid approaches to toxin detection". Thesis, University of Nottingham, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606582.
Texto completoBehrmann, Ole [Verfasser] y Gerald A. [Akademischer Betreuer] Urban. "Methods for rapid nucleic acid extraction and detection". Freiburg : Universität, 2021. http://d-nb.info/1227187289/34.
Texto completoFerrier, David Christopher. "Nucleic acid detection using oligonucleotide cross-linked polymer composites". Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28944.
Texto completoGorgannezhad, Lena. "Advanced Technologies in Rapid and Multiplex Detection of Nucleic acid". Thesis, Griffith University, 2020. http://hdl.handle.net/10072/397045.
Texto completoThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment and Sc
Science, Environment, Engineering and Technology
Full Text
Kershaw, David Michael. "Nanoparticle bound nucleic acid probes for DNA detection and gene inactivation". Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7432/.
Texto completoSaeed, Ibrahim Q. "Optoelectronically active sensitisers for the selective detection of nucleic acid biomarkers". Thesis, Cardiff University, 2016. http://orca.cf.ac.uk/100885/.
Texto completoO'Meara, Deirdre. "Molecular Tools for Nucleic Acid Analysis". Doctoral thesis, Stockholm : Tekniska högsk, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3220.
Texto completoKhater, Mohga Wagdy Yehia Mohamed. "Nanoparticle-based sensors for pathogen nucleic acid detection with interest for agriculture". Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/667373.
Texto completoThis thesis aims at developing sensitive, affordable and portable biosensors based on nanomaterials for the determination of nucleic acid related to plant pathogens. The work strives to contribute to the keeping up in the advancements of biosensing systems relevant to plant infection diagnostics which would be an essential solution in the future to the issues of plant disease monitoring and food security. Following Chapter I, state-of-the-art on the latest trends in the development of advantageous biosensors based on both antibody and DNA receptors for early plant disease detection, as well as the use of different nanomaterials such as nanochannels and metallic nanoparticles for the development of innovative and sensitive biosensing systems for the detection of pathogens (i.e. bacteria and viruses) at the point-of-care is given. The next sections of this dissertation will describe three diagnostic biosensing strategies for the detection of citrus tristeza virus (CTV) related nucleic acid using electrical and optical transducing techniques. The electrical sensing of CTV through DNA hybridization based approach and the in situ amplified nucleic acid method will be achieved on carbon sensing substrate modified with gold nanoparticles, while paper-based sensors will be operated in lateral flow format for the gold nanoparticle-based optical detection of CTV. Furthermore, all aspects of the developed biosensing systems, from the bioassay and biosensor design to their development and optimization are presented in which will be organized in the following manner: Chapter III will present highly specific DNA hybridization sensor based on AuNP-modified SPCE employing label-free impedance for the detection of the CTV-related nucleic acid, together with dedicating emphasis to the study of electrodeposition time of AuNPs, whose precise particle size and shape will be required for the enhancement of DNA hybridization rate. A set of voltammetric studies of deposited AuNPs will be discussed. Particular attention will be paid for assembling the thiolated DNA probe as sensing layer for biosensor construction. The main sensor design aspects such as AuNPs size, probe DNA concentration and immobilization time together with DNA hybridization time will be optimized, in order to precisely select the best working conditions for this diagnostic platform. Chapter IV will cover the whole process undertaken for preparation of in situ nucleic acid amplification on gold nanoparticle-modified sensor for sensitive and quantitative detection of CTV. Plant disease (Citrus tristeza virus (CTV)) diagnostics was selected as relevant target for the demonstration of the proof-of-concept. This chapter will include two parts, the first one focuses on the design of RPA amplification assay, primers design, optimization of all essential bioassay aspects such as amplification temperature, volume and screening primers and finally the electrophoresis analysis for RPA products. The second part of this chapter will demonstrate label-free highly integrated in situ RPA amplification/detection approach at room temperature that takes advantage of the high sensitivity offered by gold nanoparticle-modified sensing substrates and electrochemical impedance spectroscopic (EIS) detection. Chapter V focuses on the application of isothermal nucleic acid amplification technology in simple lateral flow platform. The preparation of AuNP-based LFA for the highly sensitive direct detection of RPA amplified nucleic acid, the assembling of lateral flow step, the conjugation of AuNPs to the antibodies used for colorimetric detection, as well as the optimization of all working conditions and finally the analytical performance of the bioassay in LF will be explored. Moreover, aiming at truly achieving the point of care requirements of simple and affordable diagnostic technologies, the work here will present the possibility of amplifying nucleic acid without heat source and visual color detection. This approach would be of great potential as point of care diagnostics.
Baloda, Meenu. "Lateral Flow Nucleic Acid Biosensor for the Detection of Sexually Transmitted Diseases". Diss., North Dakota State University, 2015. https://hdl.handle.net/10365/27596.
Texto completoLibros sobre el tema "Direct nucleic acid detection"
Kolpashchikov, Dmitry M. y Yulia V. Gerasimova, eds. Nucleic Acid Detection. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-535-4.
Texto completoAstakhova, Kira y Syeda Atia Bukhari, eds. Nucleic Acid Detection and Structural Investigations. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0138-9.
Texto completoKolpashchikov, Dmitry M. y Yulia V. Gerasimova. Nucleic acid detection: Methods and protocols. New York: Humana Press, 2013.
Buscar texto completoUltrastructural methods for nucleic acid detection by immunocytology. Stuttgart: Gustav Fischer Verlag, 1999.
Buscar texto completoThiry, Marc. Ultrastructural methods for nucleic acid detection by immunocytology. Jena, Germany: Urban & Fischer, 1999.
Buscar texto completoLuo, Yunbo. Functional Nucleic Acid Based Biosensors for Food Safety Detection. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8219-1.
Texto completoD, Sobsey Mark y AWWA Research Foundation, eds. Enteric virus detection in water by nucleic acid methods. Denver, CO: AWWA Research Foundation and American Water Works Association, 1996.
Buscar texto completoLi, Tang. Development of liposome-based nucleic acid analyses for rapid detection of listeria monocytogenes. Ithaca, NY: Cornell University, 2003.
Buscar texto completoL, Wiedbrauk Danny y Farkas Daniel H, eds. Molecular methods for virus detection. San Diego: Academic Press, 1995.
Buscar texto completoSchillinger, Julia Ann. Detection of human papillomavirus by nucleic acid hybridization as an adjunct to the papanicolaou smear. [New Haven: s.n.], 1990.
Buscar texto completoCapítulos de libros sobre el tema "Direct nucleic acid detection"
Xu, Yao y Zhi Zheng. "Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification". En Methods in Molecular Biology, 187–96. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7213-5_12.
Texto completoLehmann, Marc y Roland P. H. Schmitz. "Nucleic Acid Amplification Techniques". En Modern Techniques for Pathogen Detection, 55–111. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2015. http://dx.doi.org/10.1002/9783527687978.ch3.
Texto completoMerril, Carl R., Karen M. Washart y Robert C. Allen. "Ultrasensitive Silver Based Stains for Nucleic Acid Detection". En Nucleic Acid Electrophoresis, 152–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-58924-9_5.
Texto completoDittmann, Elke, Anne Rantala-Ylinen, Vitor Ramos, Vitor Vasconcelos, Guntram Christiansen y Rainer Kurmayer. "Nucleic Acid Extraction". En Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria, 135–61. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781119332169.ch5.
Texto completoWheeler, David. "Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis". En Nucleic Acid Electrophoresis, 311–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-58924-9_13.
Texto completoFried, Michael G. y Mark M. Garner. "The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions". En Nucleic Acid Electrophoresis, 239–71. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-58924-9_10.
Texto completoSakallah, Sameer A., Robert W. Lanning y David L. Cooper. "Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis". En Nucleic Acid Electrophoresis, 193–201. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-58924-9_8.
Texto completoXu, Wentao. "Lateral Flow Nucleic Acid Biosensors". En Functional Nucleic Acids Detection in Food Safety, 245–73. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1618-9_12.
Texto completoKarcher, Susan J. "Non-radioactive nucleic acid detection systems". En Plant Molecular Biology Manual, 309–33. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0511-8_21.
Texto completoXu, Wentao. "Nucleic Acid Biosensors for Food Safety". En Functional Nucleic Acids Detection in Food Safety, 275–322. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1618-9_13.
Texto completoActas de conferencias sobre el tema "Direct nucleic acid detection"
Shen, Chuanjie, Hao Yin, Zhaoduo Tong, Shihui Qiu, Yunxing Lu, Zhenhua Wu y Hongju Mao. "Digital Microfluidic Chip Based on Direct Ink Writing For Nucleic Acid Multiplex PCR Detection". En 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS). IEEE, 2022. http://dx.doi.org/10.1109/mems51670.2022.9699738.
Texto completoUno, Takeshi, Toshihito Ohtake, Hitoshi Tabata y Tomoji Kawai. "Direct DNA detection using ion-sensitive field effect transistors (IS-FETs) based on peptide nucleic acid". En 2004 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2004. http://dx.doi.org/10.7567/ssdm.2004.i-4-4.
Texto completoBratcher, Amber R., Laurie B. Connell y Rosemary L. Smith. "Development of a direct detection method for Alexandrium spp. Using surface plasmon resonance and peptide nucleic acid probes." En 2009 IEEE Sensors. IEEE, 2009. http://dx.doi.org/10.1109/icsens.2009.5398359.
Texto completoBogdanov, Valery L., Yu-Hui Rogers, Guang Lan y Michael Boyce-Jacino. "Multicolor instrumentation for direct fluorescent detection of nucleic acids in a microchip format". En BiOS '98 International Biomedical Optics Symposium, editado por Gerald E. Cohn. SPIE, 1998. http://dx.doi.org/10.1117/12.307323.
Texto completoFan, Y., X. Chen, J. Kong y Z. Gao. "Direct Detection of Nucleic Acids by Tagging Phosphates on Their Backbones with Conductive Nanoparticles". En TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2007. http://dx.doi.org/10.1109/sensor.2007.4300534.
Texto completoRitzi-Lehnert, Marion, Jan Claussen, Eva Schaeffer, Ole Wiborg, Isabell Wick, Klaus S. Drese, Ralf Himmelreich et al. "New Lab-on-a-Chip System for Infectious Disease Analysis". En ASME 2010 8th International Conference on Nanochannels, Microchannels, and Minichannels collocated with 3rd Joint US-European Fluids Engineering Summer Meeting. ASMEDC, 2010. http://dx.doi.org/10.1115/fedsm-icnmm2010-31048.
Texto completo"DNA-nanomachines for nucleic acid detection". En Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-200.
Texto completoLin, Zhihong, Meng Wu, Shu Ren, Michaela Arbter, Martin Boehmer, Vladimir Mirsky y Otto S. Wolfbeis. "Single- and dual- near-infrared fluorescent labeled nucleic acid conjugate for nucleic acid detection". En International Conference on Sensing units and Sensor Technology, editado por Yikai Zhou y Shunqing Xu. SPIE, 2001. http://dx.doi.org/10.1117/12.440165.
Texto completoLiu, Ye, Bo Wu, Sanjida Yeasmin y Li-Jing Cheng. "Magnetoplasmonic Nanoparticles for Enhanced Nucleic Acid Detection". En CLEO: Applications and Technology. Washington, D.C.: OSA, 2021. http://dx.doi.org/10.1364/cleo_at.2021.am3c.1.
Texto completoGulyaeva, Irina V., Ekaterina V. Efimtseva, Andrei A. Rodionov, Boris S. Ermolinsky y Sergey N. Mikhailov. "Direct synthesis of 5'-nucleotides using glycosylation reaction". En XIIth Symposium on Chemistry of Nucleic Acid Components. Prague: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2002. http://dx.doi.org/10.1135/css200205312.
Texto completoInformes sobre el tema "Direct nucleic acid detection"
Castro, A. y E. B. Shera. Ultrasensitive nucleic acid sequence detection by single-molecule electrophoresis. Office of Scientific and Technical Information (OSTI), septiembre de 1996. http://dx.doi.org/10.2172/374265.
Texto completoKingsley, Mark T. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report. Office of Scientific and Technical Information (OSTI), marzo de 2001. http://dx.doi.org/10.2172/781863.
Texto completoKingsley, Mark T. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report. Office of Scientific and Technical Information (OSTI), marzo de 2001. http://dx.doi.org/10.2172/965696.
Texto completoLers, Amnon y Pamela J. Green. LX Senescence-Induced Ribonuclease in Tomato: Function and Regulation. United States Department of Agriculture, septiembre de 2003. http://dx.doi.org/10.32747/2003.7586455.bard.
Texto completoLers, Amnon, E. Lomaniec, S. Burd, A. Khalchitski, L. Canetti y Pamela J. Green. Analysis of Senescence Inducible Ribonuclease in Tomato: Gene Regulation and Function. United States Department of Agriculture, febrero de 2000. http://dx.doi.org/10.32747/2000.7570563.bard.
Texto completoFluhr, Robert y Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, enero de 2012. http://dx.doi.org/10.32747/2012.7697123.bard.
Texto completoDelwiche, Michael, Boaz Zion, Robert BonDurant, Judith Rishpon, Ephraim Maltz y Miriam Rosenberg. Biosensors for On-Line Measurement of Reproductive Hormones and Milk Proteins to Improve Dairy Herd Management. United States Department of Agriculture, febrero de 2001. http://dx.doi.org/10.32747/2001.7573998.bard.
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