Tesis sobre el tema "Diagnostics"
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Kříž, Petr. "Online vibrační diagnostika vřetene frézovacího stroje DATRON". Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2019. http://www.nusl.cz/ntk/nusl-402508.
Texto completoBazaras, Vilius. "Lėtaeigių riedėjimo guolių diagnostiniai tyrimai". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20130218_131748-24042.
Texto completoThis paper represents a review of literature which is associated with a slow speed rolling bearings diagnostics. In this work the methodology for a slow speed rolling bearings (rotational speed up to 380 rpm) experimental researches is created. Slow speed with double row self-alignment spherical roller bearings is located in drying machines “INVERFORM”. Also experimental researches have been done and analyzed in this work. The main objective of this research - to establish a reliable research method to diagnose defects and evaluate technical condition of operating slow speed spherical rolling bearings. The method has been developed based on detailed bearings vibration measurements and vibration data formats analysis, when machine works at natural operating conditions. Obtained diagnostic research findings adopted during machines routine periodic visual examination and assessment of technical condition of the bearings.
Buchtová, Blanka. "Multiparametrická diagnostika generátoru". Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2019. http://www.nusl.cz/ntk/nusl-402514.
Texto completoSoukup, Patrik. "Diagnostika točivých elektrických strojů". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-377107.
Texto completoNarmack, Samuel. "Functionalization and Evaluation of Nanoparticle Probes for the Development of a 14-Plex Diagnostic assay". Thesis, KTH, Kemi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-299949.
Texto completoThis work was a collaboration between Aplex Bio AB and Scilifelab with the aim of developing a molecular assay capable of detecting and discriminating between 14 different pathogenic targets. There are 4 chapters with focus on different goals. In chapter one a method of evaluating emissions of fluorescent nanoparticle clusters was developed. The first approach of evaluating nanoparticle emissions was to utilize click chemistry to bind nanoparticles to macroscale structures of amplified DNA targets. The second evaluated approach was the formation of aggregated complexes of nanoparticles and amplified DNA targets. The second chapter of the thesis used azide functionalized nanoparticles supplied by Aplex Bio AB to utilize azide groups as crosslinkers and use them to functionalize the nanoparticles with DBCO oligos. A hybridization-based method was then developed to quantify relative oligo densities on the nanoparticles, enabling reproducible oligo functionalization of nanoparticles, producing nanoparticle probes that can bind to DNA. The final task of chapter 2 was evaluating the binding efficiency and specificity of the developed nanoparticle probes. The third chapter of the thesis evaluated amplification of synthetic ssDNA sequences corresponding to genetic markers of 14 pathogenic targets using RCA. The goal was to confirm specificity of chosen padlock probes and corresponding synthetic targets for each pathogen. Specific amplification of each target was a prerequisite to enable detecting and discriminating between the 14 pathogenic targets. In chapter 4 the goal was to develop a cost-effective method of oligo functionalization for nanoparticles. This chapter evaluated two main approaches of using DBCO-NHS-ester reagents to perform DBCO modification of amine-oligos. The realization of this work would develop an assay that has the potential to impact the field of diagnostics on a global scale. When fully developed, the molecular assay can be modified to detect any RNA/DNA targets which enables numerous applications, making the assay a competitive diagnostic tool which can be implemented in existing microscopy systems.
Wertheim, Adolf. "Využití diagnostické sady VAG-COM PROFI v soudním inženýrství". Master's thesis, Vysoké učení technické v Brně. Ústav soudního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-232856.
Texto completoCirhanová, Iva. "Zpracování dat z online diagnostického systému". Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2021. http://www.nusl.cz/ntk/nusl-443756.
Texto completoŠkrobánek, Martin. "Diagnostický systém papírenského stroje". Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-417858.
Texto completoBlais, Jonatan. "Validité analytique et clinique en diagnostic moléculaire : étude de cas en génotypage et détection prénatale non-invasive des aneuploïdies". Doctoral thesis, Université Laval, 2020. http://hdl.handle.net/20.500.11794/66974.
Texto completoThe analytical and clinical validity of molecular diagnostic assays are regularly questioned. Among the most commonly used nuclei acid analysis modalities in clinical laboratories, genotyping by allele-specific PCR and non-invasive prenatal aneuploidy testing (NIPT) by massively parallel sequencing, represent two of the most important applications in terms of volume. Allele drop-out on the one hand, as well as identification of variables determining diagnostic performances, reference material availability and fetal fraction estimation on the other, are all examples of analytical and clinical validity issues for which both the impact, and how well they are taken into account, remain to be evaluated for each of these applications respectively. Representative cases of both applications were therefore selected in order to study certain aspects relevant to each of these issues. In accordance with the doubts raised by several authors, lack of analytical and clinical validity was noted for all aspects examined. In particular, most diagnostic errors caused by allele dropout events were due to stochastic phenomena that cannot be prevented by careful primer design, and PCR precision levels were a limiting factor for analytical and clinical validity of NIPT assays, even though this parameter is seldomly adequately quantified and reported in the literature. Although potential clinical impacts were likely modest at the population level, at the individual level, some of the impacts may nevertheless be significant. Solutions to correct some of these problems are available, while others raise more difficult challenges. The possible causes of this lack of validity affecting molecular diagnostics are partly shared with the general problem of lack of repeatability of scientific results and are partly the result of the technical complexity, relative novelty, and the historically qualitative nature of molecular genetic methods. Integrating clinical standards upstream of the discovery process could contribute to improve analytical and clinical validity of molecular diagnostic tests and possibly to increase “bench-to-bedside” translational yield.
Zhalovaga, V. O., V. A. Ivashchenko y G. I. Litvinenko. "Medical computer diagnostics". Thesis, Вид-во СумДУ, 2009. http://essuir.sumdu.edu.ua/handle/123456789/17005.
Texto completoUdovychenko, N. M. "Machinery vibration diagnostics". Thesis, Сумський державний університет, 2013. http://essuir.sumdu.edu.ua/handle/123456789/33874.
Texto completoBaade, Ingrid Annette. "Survival analysis diagnostics". Thesis, Queensland University of Technology, 1997.
Buscar texto completoHrbáček, Vlastimil. "Návrh provozních mezí pro diagnostický systém obráběcího stroje". Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-417438.
Texto completoNolting, Andreas. "Companion Diagnostics Development and Commercialization : A Case Study from the Diagnostics’ Perspective". Thesis, KTH, Hållbarhet och industriell dynamik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-170181.
Texto completoHorák, Josef [Verfasser] y Gerald A. [Akademischer Betreuer] Urban. "Microfluidic immunosensor for point-of-care diagnostics = Microfluidischer Immunosensor für patientennahe Diagnostik". Freiburg : Universität, 2013. http://d-nb.info/1123477787/34.
Texto completoMynář, Josef. "Vliv frekvenčního měniče na životnost ložisek a jejich poškození". Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2018. http://www.nusl.cz/ntk/nusl-378018.
Texto completoZamora, Gálvez Alejandro. "Nanobiosensors for diagnostics applications". Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/457643.
Texto completoThis PhD thesis describes the development of innovative nanomaterials-based platforms with interest for environment as well as other diagnostics applications. The first platform takes advantages of molecularly imprinted polymers (MIPs) and magnetic nanoparticles for electrochemical detection of contaminants. The second one consists in a lateral flow immunoassay, where quantum dots (QDs) are used as photoluminescence source and graphene oxide as quencher to perform fluorescence measurements. Chapter 1 is an introduction in the topic and shows the state of the art of nanomaterials connected to biological and non-biological receptors for bio/sensing applications. This chapter discusses how antibodies (biological receptor) and molecularly imprinted polymers (non-biological-receptors) can improve the sensitivity, stability and specificity of the bio/sensing systems for a large number of analytes and different transducer methods, ranging from optical to electrochemical techniques. In Chapter 2, the objectives of the thesis are explained. In Chapter 3 and Chapter 4, two different sensors based on magnetic nanoparticles decorated with molecularly imprinted polymer (MIP) as non-biological receptor for sulfonamide and tributyltin detection are presented. In both sensors, the electrochemical impedance spectroscopy is used as a transduction method. The first sensor (chapter 3) has a related paper in “Anal. Chem. 2016, 88, 3578−3584” and the second sensor (chapter4) is related to the paper published in “Electrochem. Commun. 2017, 10.1016/j.elecom.2017.07.007. Two MIPs are fabricated using: (i) pyrrole and methacrylic acid for sulfonamide, and (ii) EGDMA and APTS selective to tributyltin. Moreover, the fabrication methods as well as the analytical performance including their application in real samples are explained in detail in each chapter. In Chapter 5, is presented a lateral flow immunoassay whose photoluminescent properties can be modulated upon protein recognition via the photoluminescence quenching capabilities of graphene oxide (GO). The assay is intended for the detection of a model protein in human serum, that is, human immunoglobulin G, with the aim to demonstrate a virtually universal protein detection platform. The proposed system shows a low limit of detection improving the conventional lateral flow with gold nanoparticles for the detection of the same analyte in standard buffer. Also the system is able to achieve excellent limits of detection in a complex matrix such as human serum. Finally, in Chapter 6, the general conclusions and the future perspectives are discussed. In addition, annex reports all the publications resulted during the development of this PhD thesis.
Schirninger, Rene y Stefan Zeppetzauer. "Wireless On-Board Diagnostics". Thesis, Halmstad University, School of Information Science, Computer and Electrical Engineering (IDE), 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-193.
Texto completoWireless On-board diagnostics functionality, which is a future outlook to vehicle system
parameter analysis, enables measurements and controlling without the limitation of a physical
connector. Today every vehicle must by law provide the possibility to analyze engine and
emission related parameters (OBD II). The wireless connection requires a high security level
to prevent unauthorized communication establishment with the truck’s bus system. The aim
of the project is to make a survey of the available security mechanisms and to find the most
promising solutions. Furthermore, several usage scenarios and access right levels are
specified and a risk analysis of the whole system is made. The greatest challenge is the
specification and implementation of a proper key-exchange mechanism between the analyzing
device and the truck’s bus system, which is therefore carried out with the highest possible
level of awareness. Consequently several different concepts have been formulated based on
the different usage scenarios.
Olsson, Patrik. "On-board Diagnostics Framework". Thesis, Mälardalens högskola, Akademin för innovation, design och teknik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-15664.
Texto completoRicketts, K. P. M. "Nanoparticles for tumour diagnostics". Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1348030/.
Texto completoStewart, Neil Andrew. "Novel multifunctional laser diagnostics". Thesis, University of Dundee, 2013. https://discovery.dundee.ac.uk/en/studentTheses/ddee58ac-0fda-41ca-9485-c74920cf3852.
Texto completoRosa, João Pedro Maurício. "Nanobiophotonics for biomolecular diagnostics". Doctoral thesis, Faculdade de Ciências e Tecnologia, 2013. http://hdl.handle.net/10362/10157.
Texto completoThe main objective of this thesis was to study the fluorescence modulation induced by gold nanoparticles (AuNPs) on fluorophores nearby and/or bonded to the AuNPs’ surface through nuclei acid molecules. The understanding of the effect of distance in the spectral properties of fluorophores would allow the development of a biosensor for the characterisation of DNA and/or RNA sequences. To study the photophysics involved in the fluorescence modulation by AuNPs it was necessary to develop an experimental approach that removed the effect of the optical interference caused by the presence of AuNPs. By comparing the samples with controlled reference solutions it was possible determine the fluorescence quantum yield and fluorescence decay time of the fluorophores in the vicinity of AuNPs. During the characterisation several non-photophysical phenomena involving nanoparticles were unveiled, such as a local pH effect, coupling of the plasmonic oscillator with transition moments of the fluorophore or AuNP-induced fluorophore aggregation. The developed experimental method was applied to the study of the effect of distance in the modulation of fluorescence caused by AuNPs. Using DNA molecules as spacer, the photophysical properties of fluorophores at different distances of the surface of the AuNPs showed a distance-dependence fitting into a 1/r6 dependence. The knowledge gathered on AuNP-DNA-fluorophore systems allowed for a successful semi-quantitative detection of RNA in solution. The same system showed to be useful for the simultaneous quantification and control RNA synthesis in vitro. In situ detection and gene silencing was demonstrated by targeting EGFP mRNA as proof-of-concept. A similar approach was successfully achieved in siRNA and endogenous miRNA targets. The application of this system to micro-deletions and RNA isoforms analysis was also demonstrated in synthetic targets.
Fundação para a Ciência e Tecnologia - (SFRH/BD/43320/2008)
Lindborg, Niklas. "Diagnostics of Intermittent Errors". Thesis, Uppsala universitet, Tillämpad materialvetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-453926.
Texto completoIntermittenta fel definieras som fel som ”kommer och går” i ett maskinsystem under dess livslängd och de har ett rykte att vara bland de svåraste felen att diagnostisera. Fel av intermittent karaktär existerar ofta oupptäckta trots att det har uppskattats att mer än 80% av det totala antalet fel i komponenter är intermittenta fel. Tidigare forskning om intermittenta fel tyder på att intermittenta fel, över tid, i princip alltid leder till permanenta fel. Det verkar dessutom finnas en stor kunskapslucka angående effekten och systempåverkan av intermittenta fel, både inom den akademiska världen och i näringslivet. Vidare kan termen "Inget fel hittats" ha skapat en acceptans-kultur gällande fel i komponenter som intermittenta fel kan ha orsakat. Detta examensarbete syftar till att utveckla en allmän algoritm för diagnostik av intermittenta fel. Algoritmen ska möjliggöra tidig identifiering av sensorer som håller på att gå sönder eller om de intermittenta felen orsakar för stor systempåverkan, vilket är speciellt viktigt i slutet av sensorernas livslängder. Det är önskvärt att Scania effektivt kan identifiera komponenter med intermittenta fel för att spara underhållskostnader och för att hålla kundnöjdheten hög. Flera intermittenta feldetektering- och diagnostikmetoder har utvecklats och testats med hjälp av simuleringar i MATLAB och Simulink. Tre sensorer studerades i detta examensarbete. Sensorerna var avgasmottryck sensorn, hög temperatur sensorn och NOx-sensorn. Avgasmottryck sensorn var en analog sensor medan hög temperatur- och NOx sensorn var digitala sensorer. Dessutom hade alla sensorer olika kommunikationsprotokoll och självdiagnostik möjligheter. För att effektivt kunna utveckla algoritmen kartlades all relevant diagnostik hos de tre sensorerna för att kunna avgöra vilken typ av fel som inte upptäcks av dagens diagnostik. Detta gjordes bland annat genom att studera interna Scania dokumentation och genom att intervjua dem ingenjörer som var ansvariga för den specifika sensorn. De utvecklade algoritmerna fokuserade på att diagnosera dem typer av fel som inte riktigt fångades upp av dagens diagnostik. Under examensarbetets gång identifierades tre kunder av algoritmen, alla med olika krav och önskemål på vad algoritmen ska leverera. Den första kunden är verkstadsarbetaren. De vill att algoritmen ska ge tydliga instruktioner gällande hur det upptäckta felet ska repareras. Den andra kunden av algoritmen är utvecklingsingenjörerna hos Scania. De vill ha statistik och information från algoritmen som kan användas för att få mer kunskap om intermittenta fel. Den kunskapen skulle kunna användas för att utveckla algoritmerna samt för att göra design ändringar i motorn eller sensorerna för att minska förekomsten av intermittenta fel. Den sista kunden av algoritmen är de lagstiftande myndigheterna. De vill att algoritmerna ska varna föraren av lastbilen om intermittenta fel hittas som kan påverka utsläppen samt om säkerheten har blivit försämrad. Alla dessa kunder togs hänsyn till när algoritmerna utvecklades. Resultaten tyder på att de viktigaste faktorerna att ta i beaktande vid utveckling av algoritmer för intermittent fel diagnostik är sensorns självdiagnostik och kommunikationsprotokoll. Vidare tyder resultatet från litteraturstudien att de signal symptom som intermittenta fel kan orsaka är toppar och dalar, oscillation, offset, dämpning, överkänslig signal status nedgradering, ingen signal eller maximum/minimum signal. Orsakerna till dessa symptom varierar mellan lösa/glappande kontakter i lödfogen eller kablaget, komponent åldring, oxidation, fukt, läckage eller föroreningar. Ingen ensam algoritm kan detektera alla dessa möjliga symptom i sensorns signaler, därför utvecklades fem olika detektionsmetoder, varje detektionsmetod kan upptäcka olika typer av fel. Tyvärr utvecklades inga detektionsmetoder som kunde hitta intermittenta offset eller dämpningar. Om algoritmerna implementeras på det sättet som föreslagits i detta examensarbete kan kunskapsluckan fyllas och alla kunder av algoritmen kommer att bli nöjda. Detta görs genom effektiv felisolering, insamling av värdefull information och generering av felkoder om de intermittenta felens påverkan är för stor eller om sensor håller på att gå sönder. Detta skulle möjliggöra proaktiv reperation eller utbyte av sensorer som är på väg att gå sönder. Insamlingen av information rörande intermittenta fel kan Scania använda för att öka kunskapen för att ytterligare förbättra algoritmerna för bättre detektion av intermittenta fel, vilket skulle resultera i ökad prestanda för alla Scania fordon.
Potocký, Matej. "Diagnostika závad u automobilu". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-377085.
Texto completoGadji, Macoura. "Caractérisation de deux anneaux dérivés du chromosome 22 découverts en période prénatale à l'aide de techniques de cytogénétique et de génétique moléculaire". Thesis, Université Laval, 2005. http://www.theses.ulaval.ca/2005/22427/22427.pdf.
Texto completoObjective: Cytogenetic and molecular genetic characterization of two constitutional ring chromosomes 22 identified during prenatal diagnosis. Materials and Methods: A 39 year-old woman, G4P2A1, had amniocentesis at 163/7 weeks of gestation. Conventional and molecular cytogenetic studies with microsatellite analysis of the fetal and parental cells were performed. Results: The fetus had two ring chromosomes derived from chromosome 22 with three breakpoints: one located at the centromere, another, at the p11.2 subband and the third, at the q13.31 subband. The distal part of the two derivative chromosomes was lost. Then, two rings resulted: a small and a large one. The small ring was formed by joining the end of p11.2 subband to a portion of the centromere; the other by joining the second part of the centromere to the end of q13.31 subband. The male fetus presents the following karyotype: 47,XY,r(22)(p11.1p11.2),+r(22)(q11.1q13.31). The proband’s chromosome aberration occurred de novo from the maternal chromosome. At the autopsy, the fetus showed minor clinical features. The number of fetal nucleated blood cells detected in peripheral maternal circulation, showing positive signals for Y chromosome and DiGeorge/VCF.TUPLE1 probes and absence of ARSA control signal, was 10 cells per mL. Conclusion: Despite the haploinsufficiency of many active genes, the fetus showed minor congenital malformations.
Nygren, Malin. "Molecular diagnostics of infectious diseases". Doctoral thesis, KTH, Biotechnology, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2906.
Texto completoIn DNA-based diagnostics, the polymerase chain reaction(PCR) is the most widely used DNA amplification method. Toenable both sensitive and specific detection of agents causinginfectious diseases, the PCR needs to becombined with methodsto prepare the clinical sample containing the genetic materialof the pathogen. Furthermore, methods for detection and DNAsequence analysis of the PCR amplification products are needed.This thesis describes the development of integrated systems fordetection, quantification and characterization ofmicroorganisms.
An immunomagnetic separation (IMS) technique has been usedto isolateBordetella pertussisfrom nasopharyngeal aspiratesamples. The post-PCR detection and typing ofBordetellaspp. was performed by a combination ofrestriction enzyme analysis of the amplified pertussis toxin(PT) promoter region and a solid-phase colorimetric detectionsystem; detection of immobilized amplified nucleic acid(DIANA). To investigate whether this approach could be used forreliable discrimination between the threeBordetellaspp. infecting humans, the PT promoter regionused for diagnostics was sequenced in 33 strains. To determinethe DNA sequence of this polymorphic and repetitive region, anew technique, bidirectional pyrosequencing, was utilized. Thisprocedure was used to resolve the sequence of this DNA region,which is able to form stable secondary structures inconventional Sanger DNA sequencing. A quantitative assay usingcompetitive PCR and the DIANA detection technique was alsodeveloped, for quantification ofB. pertussisin clinical samples.
By arbitrary PCR, a DNA sequence apparently specific forVibrio choleraeO139 Bengal was isolated andcharacterized. A nested PCR assay was developed for sensitiveand specific detection ofV. choleraeO139 Bengal in clinical samples and inenvironmental water samples, where differentiation betweenV. choleraeO139 Bengal andV. choleraO1 is of epidemiological interest.
The magnetic separation approach was also used to capturehuman immunodeficiency virus (HIV-1) RNA from patient plasma. Anested reverse transcription (RT)-PCR with four internalcompetitors was combined with electrophoretic separation andquantification of the PCR amplicons on an automated DNAsequencer. From the internal calibration curve, the amount ofHIV-1 RNA in the sample could be determined. Furthermore, aprimer extension assay was combined with detection andquantification of the competitive PCR products by the samebiochemiluminescent detection technique that is used inpyrosequencing. Quantification of HIV-1 viral load hasimplications in monitoring of antiretroviral therapy and inassessment of disease progression into AIDS.
Key words:bioluminescence,Bordetella, competitive PCR, DNA sequencing, humanimmunodeficiency virus type 1, PCR, pyrosequencing, solid-phasetechnology,Vibrio cholerae
© Malin Nygren, 2000
Jin, Chuan. "Investigation of Diagnostics for Buses". Thesis, Uppsala University, Department of Information Technology, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-129476.
Texto completoAs vehicle functions are getting more complex, the difficulty of diagnosing work is increasing. Currently, at Volvo Bus Corporation, there are two main methods to do fault tracing on the body, but there is no guided diagnostics, which means if something wrong happens, we can get a warning but we have no idea how to locate the error component efficiently, the diagnosis work depends largely on the expert’s experience.
In this thesis, the front door system of a Volvo bus is chosen to be a target system to try the model-based diagnosis method. The system is modeled with a model-based reasoning software called RODON. With the help of the tool, the model can be created, modified and simulated. Based on the model, the corresponding database can be generated automatically after some constraints are set, then we can generate the decision tree based on the database, that is what we expect from the model. And with the help of the decision tree, the mechanics can be guided to the right direction to find out the erroneous component precisely and efficiently.
The results of guided diagnostics were good and all the faults injected on the bus are detected and the guided diagnostics method was proved more efficient than other existing methods.
Based on the result presented in the thesis, we can draw a conclusion that the guided diagnostics can be integrated to the already existing method as a complement.
Onofri, Fabrice. "Diagnostics Optiques des Milieux Multiphasiques". Habilitation à diriger des recherches, Université de Provence - Aix-Marseille I, 2005. http://tel.archives-ouvertes.fr/tel-00287927.
Texto completoAkhras, Michael S. "Nucleic Acid Based Pathogen Diagnostics". Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4684.
Texto completoPatogena organismer smittas till värd organismen genom alla möjliga kontaktnätverk och skapar en mångfald olika sjukdomstillstånd. Dock är det fortfarande vanligt förekommande behandlingsbara infektiösa sjukdomar som orsakar den största hälsoförlusten, sett från ett globalt perspektiv. Bill och Melinda Gates Stiftelsen samarbetade med RAND kooperation för att forma “The Global Health Diagnostics Forum”. Deras mål var att etablera och analysera matematiska modeller för vilka effekter en ny diagnostisk metod utrustat för fältarbete skulle ha i utvecklingsländer. Resultaten var häpnadsveckande, med potentiellt miljoner av liv som skulle kunna räddas på en årlig basis. Den etablerade standarden för diagnostik av patogena bakterier har länge varit kultiveringsmedia baserad. Miljö specialiserade biologer har estimerat att mindre än 1 % av alla bakterie arter går att kultivera. Dock erbjuder genetiska analyser potentialen att kunna identifiera alla mikrober från alla de biologiska rikena. Nukleinsyrebaserade diagnostiska metoder har märkbart förbättrats över de senaste årtionden. Nya tekniker erbjuder utökad sensitivitet, selektivitet, sänkta kostnader och parallella analyser av patient prover. Dock är de flesta metoderna begränsade till standardiserade laboratoriemiljöer. För att konstruera en väl fungerande diagnostisk fältutrustning för användning i problem områden, behöver världsledande tekniker identifieras och kombineras. Fokuseringsområdet för denna doktorsavhandling har varit att utveckla och utföra nukleinsyrebaserade metoder för patogen diagnostik. Metoder och experimentella utförande applicerades på två distinkta system i) sökning av antibiotika resistens relaterade mutationer i den patogena bakterien Neisseria gonorrhoeae och ii) genotypning av det cancer orsakande Humana Papillomaviruset (HPV). Den första delen av studien inriktade sig mot utveckling av snabba, direkta och multiplexa Pyrosekvenserings baserade nukleinsyreanalyser. Med förbättrad provprepareringsmetodologi kunde vi detektera multipla HPV infektioner med högre sensitivitet än vad tidigare beskrivits med liknande metodologi. Den andra delen av studien fokuserades på multiplexa nukleinsyre amplifikationer med “Molecular Inversion Probe” tekniken med sista steg Pyrosekvenserings analys. “PathogenMip assay” erbjuder ett komplett detektionsprotokoll för alla kända patogena organismer. Vi introducerar även den nya “Connector Inversion Probe”, en “Padlock Probe” kapabel att genomföra kompletta gap fyllningar för multiplex nukleinsyre amplifiering.
QC 20100624
Moradpour, Chahaki Saeed. "On-Board Diagnostics over Ethernet". Thesis, Högskolan i Halmstad, Sektionen för Informationsvetenskap, Data– och Elektroteknik (IDE), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-19527.
Texto completoMeisingseth, Andreas. "Demodulation Techniques in Gearbox Diagnostics". Thesis, Uppsala universitet, Signaler och System, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-177275.
Texto completoVähäoja, P. (Pekka). "Oil analysis in machine diagnostics". Doctoral thesis, University of Oulu, 2006. http://urn.fi/urn:isbn:9514280768.
Texto completoDelserieys, A. "Optical diagnostics of laser plasmas". Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492152.
Texto completoBenghiat, Sonia. "Diagnostics for generalized linear models". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ64046.pdf.
Texto completoRåsbäck, Therese. "Laboratory diagnostics of Brachyspira species /". Uppsala : Dept. of Clinical Sciences, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/200769.pdf.
Texto completoSalamat, Reza. "Gas path diagnostics for compressors". Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/7889.
Texto completoMoustakas, John. "Spectral Diagnostics of Galaxy Evolution". Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/305142.
Texto completoGlasgow, Craig I. "Mixing diagnostics using particle tracking". Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244528.
Texto completoKremer, Clemens. "Improved diagnostics for sleeping sickness". Thesis, University of Glasgow, 2013. http://theses.gla.ac.uk/4822/.
Texto completoIsheden, Gabriel. "Diagnostics of Semantic Word Spaces". Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-142614.
Texto completoBuche, Silvain. "Polymer electrolyte fuel cell diagnostics". Thesis, University of Bath, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285318.
Texto completoWarnes, Alexis. "Diagnostics in time series analysis". Thesis, Durham University, 1994. http://etheses.dur.ac.uk/5159/.
Texto completoBolland, Peter James. "Robust neural estimation and diagnostics". Thesis, London Business School (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298220.
Texto completoHumphreys, Emma J. "Molecular diagnostics for cereal rusts". Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273258.
Texto completoJing, Cao. "Spray diagnostics by laser diffraction". Thesis, University of Sheffield, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321852.
Texto completoBuckley, Alastair. "Laser diagnostics of discharge lamps". Thesis, University of Sheffield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322979.
Texto completoMartino, Wenceslao Moreda. "Novel stains as malaria diagnostics". Thesis, University of Aberdeen, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401409.
Texto completoYu, Tania Weidan. "Iris imaging for health diagnostics". Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/119548.
Texto completoThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 49-51).
The development of mobile technology and machine learning tools has made it easier than ever to monitor health without visiting a doctor. In this thesis, we explore the use of iris imaging as a medical diagnostic tool. We implement a system in which images captured using a mobile device can be uploaded to and analyzed by a central server. With this platform, we hope to build a large database of standard iris images with labeled medical data and facilitate studies of iris diagnostics. In our implementation, the feature extraction and classification tools built are applied to predict diabetes, through a study conducted in collaboration with researchers at Swami Vivekananda Yoga Anusandhana Samsthana (SVYASA). The results show improvement in prediction accuracy and encourage further development of the server platform for future, large-scale studies.
by Tania Weidan Yu.
M. Eng.
Treppo, Steven. "Physical diagnostics of cartilage degeneration". Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/85263.
Texto completo"January 1999."
Includes bibliographical references (leaves 219-239).
by Steven Treppo.
Ph.D.
Sobel, Adam H. 1967. "Quantitative diagnostics of stratospheric mixing". Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/57961.
Texto completoIncludes bibliographical references (p. 124-134).
by Adam Harrison Sobel.
Ph.D.