Literatura académica sobre el tema "Développement de l'ovocyte"
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Tesis sobre el tema "Développement de l'ovocyte"
Labrecque, Rémi. "Analyse du transcriptome de l'ovocyte bovin en lien avec la compétence au développement". Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/25747.
Texto completoIn this study, we specifically looked at the mRNA modulations observed in the bovine oocyte in regard of its potential to produce an embryo. We first used a model where the oocyte quality could be modulated in vivo, using ovarian stimulation. We were able to show that the oocyte quality acquired during that period will not last and we proposed a possible mechanism of RNA degradation that could be implicated in the decrease of oocyte quality. In order to better understand the importance of the LH support for the oocyte at the end of follicle growth, a GnRH antagonist was used during the period where the oocyte quality is gained. Although the suppression of the LH secretion did not have a significant impact on the potential of the oocyte to produce an embryo, important cellular functions such as translation regulation were affected in the oocyte by the treatment. The transcriptome analysis of the oocyte collected from follicles of gradually increasing sizes has allowed us to better understand the sequential changes undergoing in the oocyte during the follicular growth. An important and gradual modulation of several transcripts involved in chromatin remodeling was also identified, illustrating the importance of this process in the gradual developmental potential acquisition. Considering the major chromatin remodeling of the germinal vesicle that is known to occur towards the end of oocyte growth, we decided to analyse the transcriptome of these oocytes, grouped according to their degree of chromatin compaction. Although an important decrease of the transcriptional activity will occur in parallel with the chromatin condensation, we were able to observe the accumulation of several histone mRNA over this period, suggesting a potential storage to fulfill the first few cell cycles. This project has allowed us to better define the molecular changes undergoing in the oocyte during its growth, where the oocyte quality is significantly modulated. It was also possible to propose different hypothesis in order to explain the underlying biological causes of this decrease quality.
Blondin, Patrick. "Effet de la morphologie du follicule et de l'ovocyte sur la compétence au développement embryonnaire bovin". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25219.pdf.
Texto completoThelie, Aurore. "Gènes préférentiellement exprimés dans l'ovocyte bovin : régulation des transcrits au cours de la maturation ovocytaire et du développement embryonnaire préimplantatoire". Thesis, Tours, 2008. http://www.theses.fr/2008TOUR4034.
Texto completoMeiotic resumption and early embryo development rely on the precise control of the stability and translation of transcripts accumulated in the oocyte cytoplasm, including oocyte-specific transcripts. This work was to improve the understanding of molecular aspects of oocyte quality in bovine. In one hand, we have characterized novel genes expressed preferentially in the oocyte. In particular, we report expression of Stem loop binding protein 2 for the first time in mammals; it could be involved in controlling histone synthesis as in Xenopus. In the other hand, using both global and targeted approaches, maternal transcripts were shown to undergo moderate degradation during maturation, but they displayed contrasted deadenylation profiles. Most oocyte-specific genes were not reactivated in the embryo. Overall, this work paves the way for the characterization of an expression profile associated with the oocyte competence to support the embryo development
Arnoult, Christophe. "Canaux ioniques et premières étapes du développement embryonnaire : études électrophysiologiques par la technique du patch-clamp de l'ovocyte du Tunicier Ciona intestinalis". Université Joseph Fourier (Grenoble ; 1971-2015), 1994. http://www.theses.fr/1994GRE10085.
Texto completoZiyyat, Ahmed. "Séparation de spermatides rondes par cytométrie en flux et expression génique durant le développement préimplantatoire après microinjection de la spermatide dans l'ovocyte de souris". Paris 11, 2000. http://www.theses.fr/2000PA11T062.
Texto completoVaur, Sabine. "Etude de la maturation méiotique de l'ovocyte d'axolotl et analyse de la régulation post-transcriptionnelle des gènes Wnt au cours de l'ovogenèse et du développement précoce". Paris 6, 2004. http://www.theses.fr/2004PA066329.
Texto completoDelelis, Charlotte Fanien. "Mise en évidence d'un rôle spécifique de la protéine HSP70 dans le contrôle de la transcription par l'ARN polymérase II dans l'ovocyte d'amphibien". Paris 5, 1998. http://www.theses.fr/1998PA05S024.
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