Tesis sobre el tema "Cucumber plant"
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Tungadi, Trisna Dewi. "Cucumber mosaic virus modifies plant-aphid interactions". Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708288.
Texto completoWestwood, Jack Henry. "Cucumber mosaic virus infection and plant-aphid interactions". Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608811.
Texto completoBalcı, Evrim Doğanlar Sami. "Genetic characterization of cucumber mosaic virus(CMV)resistance in tomato and pepper". [s.l.]: [s.n.], 2005. http://library.iyte.edu.tr/tezler/master/biyoloji/T000388.pdf.
Texto completoSejbl, Iva Charlotte. "Blue light-signal transduction in etiolated cucumber hypocotyls". Thesis, University of York, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316140.
Texto completoSoards, Avril Jacqueline. "The Cucumber mosaic virus 2b protein : influences on the plant-virus interaction". Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619971.
Texto completoAfsharifar, Alireza. "Characterisation of minor RNAs associated with plants infected with cucumber mosaic virus". Title page, table of contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09pha2584.pdf.
Texto completoGraham, Ian Alexander. "Structure and function of the cucumber malate synthase gene and expression during plant development". Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/12057.
Texto completoChen, Chunquan. "Induced systemic resistance against Pythium aphanidermatum by plant growth-promoting rhizobacteria on cucumber, Cucumis sativus L". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ50129.pdf.
Texto completoChen, Chunquan 1958. "Induced systemic resistance against Pythium aphanidermatum by plant growth-promoting rhizobacteria on cucumber (Cucumis sativus L.)". Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=35862.
Texto completoRobertson, Fiona Catherine. "Selective targeting of micro RNA-regulated plant development by the 2b protein of Cucumber mosaic virus". Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613182.
Texto completoJiang, Sanjie. "CMV infection affects bumblebee pollination behaviour and plant reproductive success". Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/275637.
Texto completoFleming, Daniel. "THE SPOTTED CUCUMBER BEETLE (DIABROTICA UNDECIMPUNCTATA HOWARDI): INTERACTIONS WITH CUCURBITS AND ITS STATUS AS A SWEET POTATO PEST IN MISSISSIPPI". MSSTATE, 2009. http://sun.library.msstate.edu/ETD-db/theses/available/etd-03272009-112856/.
Texto completoNur, Sabrina Ahmad Azmi. "Studies on an effector NLP1 expressed during the late phase of plant infection by Colletotrichum orbiculare". Kyoto University, 2018. http://hdl.handle.net/2433/233850.
Texto completo0048
新制・課程博士
博士(農学)
甲第21311号
農博第2296号
新制||農||1064(附属図書館)
学位論文||H30||N5145(農学部図書室)
京都大学大学院農学研究科応用生物科学専攻
(主査)教授 髙野 義孝, 教授 田中 千尋, 教授 寺内 良平
学位規則第4条第1項該当
Walker, Jason. "Plant diversity and its effects on populations of cucumber beetles and their natural enemies in a cucurbit agroecosystem". Thesis, This resource online, 1997. http://scholar.lib.vt.edu/theses/available/etd-08222008-063552/.
Texto completoLiu, Xing y s3072856@student rmit edu au. "Electrical Impedance Spectroscopy Applied in Plant Physiology Studies". RMIT University. Electrical and Computer Engineering, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080428.092529.
Texto completoChang, Peta-Gaye Suzette. "Plant Virus Diagnostics: Comparison of classical and membrane-based techniques for immunoassay and coat protein sequence characterization for Cucumber mosaic virus and three potyviruses". Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/28017.
Texto completoPh. D.
Scherf, Andrea [Verfasser], Gerhard [Akademischer Betreuer] Thiel y Cornelia [Akademischer Betreuer] Ullrich-Eberius. "Licorice, cucumber, downy mildew: tracing the secret Interactions between the plant extract, the host and the pathogen / Andrea Scherf. Betreuer: Gerhard Thiel ; Cornelia Ullrich-Eberius". Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2012. http://d-nb.info/1106115716/34.
Texto completoCórdova, Pedro Javier Mansilla. "Infecção natural e experimental de cucurbitáceas com o vírus do mosaico do mamoeiro - estirpe mamoeiro e implicações epidemiológicas". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-11022011-102358/.
Texto completoBesides Carica papaya, Papaya ringspot virus type P (PRSV-P) infects systemically only species belonging to the family Cucurbitaceae. Their susceptibility varies according to the species/cultivar, virus isolate and the method of inoculation. Attempts to recovery PRSV-P from naturally infected cucurbit plants grown near to or among diseased papaya trees have shown distinct results worldwide. This study aimed to evaluate the susceptibility of Cucurbita pepo cv. Caserta, Cucurbita maxima cv. Exposiçao, Cucumis sativus hybrid Primepack Plus, and Citrullus lanatus cv. Crimson Sweet to five isolates of PRSV-P obtained from different regions of Brazil. It was also evaluated the natural infection of cucurbit plants grown between rows and in the vicinity of papaya trees infected with PRSV-P. The five PRSV-P isolates were maintained in papaya plants. Cucurbit plants grown in pots under greenhouse conditions were mechanically inoculated with each isolate at the cotiledonary stage. The plants were assessed based on symptoms and infection was confirmed by PTA-ELISA using extracts from the inoculated cotyledons and upper leaves. The same extracts were also mechanically inoculated on papaya plants in order to recover the virus isolate. Inoculated papaya plants were also tested by PTA-ELISA. Zucchini squash was the most susceptible species to PRSV-P, followed by watermelon and cucumber. Pumpkin cv. Exposição was not infected. To study the natural infection of zucchini squash cv. Caserta by PRSV-P, a trial was carried out in Linhares, State of Espírito Santo; three independent trials were carried out in Rinópolis; and four trials were carried out in Piracicaba, both regions located in the State of São Paulo. Watermelon and cucurbit were also included in some trials in Piracicaba. After approximately 40 to 70 days, leaf samples were collected and tested individually or in groups of three to five plants for the presence of PRSV-P by mechanical inoculation on papaya plants under greenhouse conditions. None of the zucchini squash plants grown between rows of infected papaya trees in Linhares was found infected by PRSV-P based on the virus recovery test to papaya plants. The virus was also not recovered from watermelon and cucurbit plants grown between rows of infected papaya trees in Piracicaba. On the other hand, PRSV-P was recovery from zucchini squash plants grown intercalated with diseased papayas in Rinópolis and Piracicaba. The number of infected plants varied among the trial. Several attempts to recover PRSV-P from innumerous zucchini squash plants grown approximately five to 80 meters from diseased papaya trees in Piracicaba failed. Alates of several species of aphids were captured in the field at Piracicaba. Also, innumerous cucurbit plants were found infected by the potyviruses Papaya ringspot virus type W and Zucchini yellow mosaic virus, suggesting aphids activity in the area. The results confirmed the variable susceptibility of cucurbit species to infection with PRSV-P. Although natural infection with PRSV-P was restricted to zucchini squash cv. Caserta grown among infected papaya trees, the presence of cucurbit plants in the vicinity of papaya orchards, especially where disease control is done by systematic rouging of diseased plants, should not be allowed.
Bonaldo, Solange Maria. "Efeito de Saccharomyces cerevisiae na síntese de fitoalexinas em sorgo, na germinação e formação de apressórios por fungos fitopatogênicos e na proteção de pepino a Colletotrichum lagenarium e sorgo a Colletotrichum sublineolum". Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-16062005-135554/.
Texto completoThe yeast Saccharomyces cerevisiae has potential in the control of diseases in some plants by the ability to induce resistance and elicits defence mechanisms. However, in the process of purification of elicitor (s) present in the cell wall of S. cerevisiae a low efficiency was observed during the process, making difficult to carry out experiments, mainly in field. Thus, to optimize the process of extraction of the elicitor (s) present in the cell wall of the yeast, cells in suspension were autoclaved by minutes or hours, once or in sequence. After autoclaving the carbohydrate and proteins content of these preparations were determinate and they were tested in phytoalexin accumulation in sorghum mesocotyls, previously treated or not with the abrasive carborundum, and conidia germination and appressorium formation by Colletotrichum lagenarium and Colletotrichum sublineolum. Because of the higher carbohydrate content and the highest elicitor activity in sorghum mesocotyls, the preparation autoclaved by 4 hours in sequence was selected for the purification process. Ion exchange chromatography (IEC) using Tris-HCl or ammonium bicarbonate buffer for column elution, were used to separate fractions with higher elicitation activity from those exhibiting lower elicitation activity. Fractions from the chromatography obtained using either buffer induced the accumulation of phytoalexin in sorghum mesocotyls, previously treated or not with the abrasive carborundum. However, the fractions from the chromatography with ammonium bicarbonate buffer were able to inhibit in 100% the conidia germination and appressorium formation by the phytopathogens. In the protection of cucumber in growth chamber, there was a reduction in symptoms only when the cucumber seedlings were treated with the fractions from the IEC with Tris-HCl buffer, two days before the inoculation with the fungus. In greenhouse, fraction from the IEC with ammonium bicarbonate buffer was able to protect cucumber seedlings against C. lagenarium, but without increase peroxidases activity. Sorghum plants treated with the fractions, obtained using either buffer, exhibited reduced infection in the treated leaves and leaves just above, with phytoalexin production. The highest phytoalexin production in sorghum plants was observed in leaves treated with the crude preparation of the yeast autoclaved by 4 hours sequentially. The results of the present study indicate the presence of termoestable compounds in the cell wall of the yeast, released in higher concentrations in function of the sequential autoclavage, that are able to induce phytoalexin accumulation in sorghum mesocotyls and leaves, with antifungal activity on C. sublineolum and C. lagenarium and potential to induce local resistance in cucumber against C. lagenarium and local and systemic resistance in sorghum against C. sublineolum.
Pinto, Zayame Vegette. "Efeito da origem dos isolados do Cucumber mosaic virus (CMV) e da presença de dois Potyvirus na transmissão do CMV para abobrinha de moita por meio de duas espécies de afídeos". Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-21052004-140518/.
Texto completoThe cucurbits in Brazil can be infected by different viruses, such as Papaya ringspot virus - type W (PRSV-W); Zucchini yellow mosaic virus (ZYMV) and Cucumber mosaic virus (CMV). The first two belong to the genus Potyvirus and in general they occur more frequently than CMV, which is a species of the genus Cucumovirus. The two potyviruses and the cucumovirus are transmitted by means of aphids in a non persistent way. The main objective of this work was to obtain subsidies that can explain the lower incidence of CMV in cucurbit species, studying: (a) the interference of the potyviruses PRSV-W and ZYMV in the transmission of CMV by means of Aphis gossypii and Myzus persicae to zucchini squash plants (Cucurbita pepo 'Caserta') and (b) the effect of isolates of CMV from passion flower (Passiflora edulis f. flavicarpa), bell pepper (Capsicum annuum), cucumber (Cucumis sativus), melon (Cucumis melo) and Commelina virginica in the infectividade of zucchini squash plants through the transmission by aphids. To evaluate the possible interference of the potyvirus in the transmission of CMV, zucchini squash plants were inoculated with aphids that acquired each one of the viruses separately; CMV simultaneously with each one of the potyvirus; one of the potyvirus follow by CMV and vice-versa. The results showed that the transmission of PRSV-W, ZYMV and CMV separately was more efficient than in mixture. The potyviruses in general were more efficiently transmitted by both species of aphids than CMV. When in mixture (simultaneous or sequential acquisition), there was a reduction in the rate of transmission of CMV as well as that of the potyvirus present in the mixture. The evaluation on the effect of the origin of the isolate of CMV in the infectivity of zucchini squash showed that only the isolate from bell pepper did not infected the plants when inoculated by means of A. gossypii and M. persicae. This isolate also did not infecte zucchini squash when inoculated mechanically. The others isolate infected zucchini squash when transmitted by both species of aphids. Analysis of the capsidial protein of the different isolates of CMV indicated that all presented the same mobility in SDS-PAGE. The origin of the isolate of CMV, the efficiency of the species of aphid and the interference of the potyviruses PRSV-W and ZYMV on its transmission can partly explain the lower incidence of this cucumovirus in cucurbits species in Brazil.
Juraeva, Dilafruz. "Study the possible mechanisms of plant growth promotion by wheat diazotrophic bacteria grown in Uzbekistan soil". Doctoral thesis, Humboldt-Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2011. http://dx.doi.org/10.18452/16326.
Texto completoPlant growth promoting bacteria (PGPB) are ubiquitous in both plant root and shoot, and are important contributors to the nitrogen-input of plants exerting their positive effects on plant growth directly or indirectly through different mechanisms. The present work focuses on a) the isolation of PGPB, which promotes the growth of different plant cultures and controls plant diseases caused by Fusarium species, b) the prospects of PGPB to solve plant nutritional problems, c) developing new molecular methods for the assessment of their diversity and activity. In the frame of this thesis, the methods for the description of the diversity of root colonizing PGPB have been developed and improved to provide links between introduced PGPB abundance and activities. The approach used was based on the sensitive real – time PCR detection/quantification of introduced PGBP and the nitrogenase reductase gene (nifH), which served as a marker gene for potential diazotrophs. The amplified 16S-23S ISR sequences of studied bacteria were subjected to strain – specific primer design and a highly specific bacteria quantification protocol were developed. The bacteria quantification protocol was based on real – time PCR using strain specific primers in order to evaluate the colonization ability of studied bacteria, which were inoculated to plant roots. The results presented in this thesis have shown that monitoring of nifH amount in plant root is a suitable and promising approach to link inoculated diazotrophic bacteria abundance and its potential activity. The study of nifH gene abundance in plant offers the opportunity to identify key players in asymbiotic nitrogen fixation, to study short-term community responses in changing environments, or to analyze the effect of regulation in situ.
Crawford, T. W. Jr, J. L. Stroehlein y R. O. Kuehl. "Manganese Stresses and Mineral Nutrition of Cucumber Plants". College of Agriculture, University of Arizona (Tucson, AZ), 1987. http://hdl.handle.net/10150/221402.
Texto completoCazier, Jean-Baptiste. "Mathematical modelling of gas exchanges in film-wrapped cucumbers /". Alnarp : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2000. http://epsilon.slu.se/avh/2000/91-576-5763-7.pdf.
Texto completoGambley, Rhonda Leigh. "Physiological studies in the development of a transformation system for cucumis sativus L. (cucumber)". Thesis, Queensland University of Technology, 1990. https://eprints.qut.edu.au/35959/1/35959_Gambley_1990.pdf.
Texto completoWahyuni, Wiwiek Sri. "Variation among cucumber mosaic virus (CMV) isolates and their interaction with plants". Title page, contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phw137.pdf.
Texto completoHackland, Andrew F. "The development of transgenic plants resistant to cucumber mosaic virus and tobacco necrosis virus". Doctoral thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/21411.
Texto completoCucumber mosaic virus (CMV) and tobacco necrosis virus (TN V) often occur in mixed virus infections in South Africa. Both viruses are of economic importance because of their world-wide distribution, extensive host range and their effects on yields of agriculturally important crop plants. The complete cDNA sequences of CMV-Wemmershoek (CMV-Wem) coat protein (CP) and TNV-F5P CP genes were cloned and subjected to sequence analysis. CMV-Wem is closely related to CMV-WL and CMV-Q, and therefore falls into CMV subgroup II. Similar analysis showed that TNV-F5P is closely related to TNV-A. By characterizing and sequencing these clones the authenticity of the CMV and TNV CP genes was also determined, prior to sub cloning into the appropriate vectors for expression in E. coli and tobacco. Constructs containing both the full-length CP genes of CMV-Wem and TNV-F5P were subcloned in frame with the malE gene, encoding the maltose binding protein (MBP), in the IPTG-inducible pMALTM vector system, and expressed in E. coli. Through immunological detection the authenticity of both CPs was confirmed. The CMV CP translation product expressed in E.coli was used as an antigen to raise antiserum free from contaminating plant host-specific antibodies. The CP genes of both viruses were individually cloned in both orientations (sense and antisense) in Agrobacterium tumefaciens Ti-plasmid-based binary and cointegrate vectors. The study was then extended to include engineering doubly transgenic plants. In order to determine whether the full-length CP is required to mediate virus resistance, a truncated form of the TNV CP was generated by deleting 83 amino acids from the C-terminus. Transgenic Nicotiana tabacum cv Petit Havana SRl plants containing one of a number of different forms of CMV and TNV CP nucleotide sequence were generated. In whole plant studies, mechanical inoculation of Ro lines with CMV-Wem resulted in more than 50% of the CMV CP-sense (CP+) and CP-antisense plants not developing visible systemic disease symptoms. In both the CMV CP+ and doubly transgenic plants CMV-Wem accumulation was delayed, but virus was found to accumulate in the inoculated leaves over time. The CMV CP+ lines showed excellent protection against CMV-Q, but showed only a delay in symptom production when inoculated with CMV -Y, from subgroup I.
Lewsey, Mathew Graham. "Effects of the Cucumber mosaic virus 2b gene and other viral sequences in transgenic plants". Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614169.
Texto completoReynolds, Susan Jennifer. "Structure of the isocitrate lyase gene of cucumber and its transcriptional regulation in higher plants". Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/11304.
Texto completoMasiri, Jongkit Murphy John F. "The nature of cucumber mosaic virus-induced symptoms in bell pepper (Capsicum annuum L.)". Auburn, Ala., 2009. http://hdl.handle.net/10415/1977.
Texto completoKalogirou, Maria. "Antiviral and quality effects of chemical elictors and Cucumber Mosaic Virus (CMV) infection on tomato plants and fruits". Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/7278.
Texto completoPelinganga, Osvaldo Manuel. "Developing phytonematicides using indigenous cucumis africanus and cucumis myriocarpus fruits for tomato production systems". Thesis, University of Limpopo, Turfloop Campus, 2013. http://hdl.handle.net/10386/1286.
Texto completoGlobal withdrawal of synthetic fumigant and non-fumigant nematicides due to their ecounfriendly impacts and high toxicity to non-target organisms, respectively, increased the research and development of alternatives for managing population densities of plantparasitic nematodes, particularly the root-knot (Meloidogyne species) nematodes. Although Meloidogyne species had been managed using genotypes that are resistant to plant-parasitic nematodes in various crops, various challenges negate the available or introgressed nematode resistance. In tomato (Solanum lycopersicum) production, nematode races and instability of nematode resistant genotypes under certain conditions necessitated the continued research and development of alternatives since most of the existing commercial tomato cultivars are highly susceptible to various biological races of Meloidogyne species. The aim of the study was to research and develop appropriate dosages of two phyto- nematicides which could be applied through drip irrigation system in open field tomato production systems, while the specific objectives were to: (1) determine whether a computer-based model could provide nonphytotoxic concentrations to tomato plants using fresh fruits of wild watermelon (Cucumis africanus) and wild cucumber (C. myriocarpus) under greenhouse conditions, (2) determine whether computer-based concentrations from the two plant species when using dried fruits would be less phytotoxic and more suppressive to nematodes, (3) investigate application time intervals for the two products, (4) determine responses of plant growth in tomato and nematode suppression in respect to the derived dosages, and and (5) validate dosages of fermented crude extracts from the two plant species with respect to plant growth of tomato and suppression of nematode numbers. xxxiii Greenhouse, microplot and field studies were set to test the hypotheses intended to achieve the stated objectives, with reliability of measured variables being ensured by using statistical levels of significance (P ≤ 0.05) and coefficients of determination (R2), while validity was ensured by conducting experiments at the same location over two seasons and/or by setting up factorial treatments. Firstly, fermented plant extracts of fresh fruits from C. africanus and C. myriocarpus consistently reduced population densities of Meloidogyne species by 80-92% and 50-90%, respectively. Tomato plants were highly sensitive to the two products as shown by the total degree of sensitivities (Σk) and biological index of 0 and 3, respectively. Also, the mean concentration stimulation range (MCSR) of 11% and 7% concentrations, respectively, attested to this phytotoxicity. Secondly, fermented crude extracts of dried fruits from C. africanus and C. myriocarpus also reduced population densities of Meloidogyne species by 78-97% and 87-97%, respectively. Tomato plants were highly tolerant to the two products in dried form as shown by the total degree of sensitivities (Σk) and biological index of 4 and 3, respectively. The MCSR values for C. africanus and C. myriocarpus dried fruits on tomato were 2.64% and 2.99%, respectively, which for the purpose of this study were individually adjusted to 3%, which translated to 36 L undiluted material/ha of 4 000 tomato plants. In subsequent studies, 3% concentration was used as the standard, along with double strength concentration, namely, 6% concentration. Thirdly, the MCSR values derived in Objective 4, namely 3% and 6% concentration for both Cucumis species using the CARD model were used in the optimisation of application time interval using the innovative concept of weeks (0, 1, 2, 3 and 4) in a 30-day month period. Application time interval for 3% and 6% concentrations of C. africanus fruits was xxxiv optimised at 2.40 and 2.61 weeks in a 30-day month period, respectively, which translated to 18 days [(2.4 weeks/4 weeks) × 30 days] and 20 days [(2.6 weeks/4 weeks) × 30 days], respectively. In contrast, for both concentrations from fermented crude extracts of C. myriocarpus fruits, application time interval was optimised at 16 days for 2.2 and 2.1 weeks, respectively. During optimisation of application frequencies, fermented crude extracts from C. africanus and C. myriocarpus reduced final population densities of M. incognita race 2 by 70-97% and 76-96%, respectively. Fourthly, optimum application intervals (time), allowed computation of dosage, which is a product of concentration and application frequency (dosage = concentration × application frequency). Fifthly, validation of the dosages under open field conditions suggested that 6% × 16-day dosage under crude extracts from C. myriocarpus fruit significantly (P ≤ 0.05) improved growth of tomato plants when compared with those of either 0% (untreated control) or 3% at 16 days. In contrast, dosages of C. africanus fruit at two application frequency had no effect on growth of tomato plants – suggesting that either of the dosages was suitable for use in tomato production since both reduced nematode numbers. During validation, the materials reduced nematode numbers by margins similar to those observed previously under other environments. In conclusion, crude extracts of the two Cucumis species have stimulatory concentrations which have potential similar reductive effects on population densities of Meloidogyne species and could serve as botanical nematicides. However, since plant responses to the two products differed in terms of their respective dosages and active ingredients, it implied that for further improvement of the two, the overriding focus should be on their interaction with the protected plants and nematode numbers. Ideally, future research xxxv should include environmental impact studies, especially on the influence of the products fruit quality of tomato, earthworms, fish and bees.
Rocha, Rita de Cassia Dosciatti Serrão. "Quitosana na indução de resistência ao tombamento de plântulas de espécies olerícolas e no controle de fitopatógenos in vitro". Universidade Tecnológica Federal do Paraná, 2015. http://repositorio.utfpr.edu.br/jspui/handle/1/1617.
Texto completoA indução de resistência é conceituada como a ativação de um estado de resistência contra doenças, o qual é induzido sistemicamente em plantas pela utilização de agentes bióticos ou abióticos, sem qualquer alteração do genoma da planta, ocorrendo de maneira não específica, por meio da ativação de genes que codificam para diversas respostas de defesa vegetal. A quitosana é um polímero derivado da desacetilação da quitina, encontrada em grande quantidade na carapaça de crustáceos, sendo estudada com potencial para controle fitopatógenos, tanto para sua ação fungistática direta, quanto pela capacidade de induzir a defesa das plantas, indicando a presença de moléculas com características elicitoras. Foram desenvolvidos três experimentos com objetivos de avaliar o potencial da quitosana na indução de resistência a plântulas de beterraba (Beta vulgaris), tomate (Solanum lycopersicum) e pepino (Cucumis sativus) e no controle de Fusarium sp., Rhizoctonia solani Kuhn e Pythium sp. em condições in vitro. O delineamento experimental foi inteiramente casualisado, com quatro repetições As sementes de beterraba, tomate e pepino foram submersas em solução de quitosana por 20 minutos, nas concentrações de 0,25; 0,5; 1 e 2% e na testemunha água destilada. As sementes foram semeadas em bandejas de poliestireno expandido contendo substrato lantmax Florestalr previamente esterilizado e inoculado com Fusarium sp., Rhizoctonia solani Kuhn e Pythium sp., respectivamente, para as três culturas. O experimento foi conduzido por 14 dias em câmara de cultivo com controle de temperatura (25 C 2 C), luminosidade (fotoperíodo de 12 horas) e umidade relativa (70% 10%). As avaliações realizadas foram emergência das sementes, tombamento de plântulas, comprimento de plântulas, massa da matéria fresca e atividade das enzimas fenilalanina amônia-liase (FAL), quitinases e b-1,3- glucanase. Foi avaliado tamb´em o crescimento micelial de Fusarium sp., Pythium sp. e R. solani em meio de cultura B.D.A. (Batata-Dextrose e Agar) contendo quitosana nas mesmas concentrações avaliadas nas sementes. Na cultura da beterraba, o tratamento de sementes com quitosana propiciou maior emergência e comprimento das plântulas, e reduziu o percentual de tombamento. O tratamento com quitosana ativou a resistência sistêmica adquirida com expressão das enzimas quitinases e b-1,3-glucanase. No tomate a quitosana na concentração de 0,25% favoreceu a emergência das plântulas, reduziu a incidência de tombamento e ativou as enzimas FAL, quitinases e b-1,3-glucanase. Em pepino, com concentração de até 0,5%, favoreceu a emergência das plântulas e reduziu a incidência de tombamento. A quitosana ativou as enzimas FAL e b-1,3-glucanase. A quitosana também apresentou ação fungistática sobre o crescimento inicial de Pythium sp. e Rhizoctonia solani Kuhn em condições in vitro, no entanto, tal ação não prevaleceu até o término do experimento. Para Fusarium sp. o aumento da concentração da quitosana resultou na redução do crescimento micelial in vitro.
Induction of resistance is defined as the activation of a state of resistance against diseases which is induced systemically in plants by the use of biotic or abiotic agents without any modification of the plant genome, occurring non-specific way, by activating genes coding for various plant defense responses. Chitosan is a polymer derived from the deacetylation of chitin, which is found in large quantities in crustacean shell, and studied with the potential to control plant pathogens, both by its direct fungistatic action, as the ability to induce protection of plants, indicating the presence of molecules of elicitoras characteristics. Three experiments with objective of evaluating the potential of chitosan in the seedling resistance induction were developed, beet (Beta vulgaris) seeds, cucumber (Cucumis sativus) seeds and tomato (Solanum lycopersicum) seeds, and the control of Fusarium sp., Rhizoctonia solani K¨uhn e Pythium sp. in vitro conditions. The experimental design was completely randomized, with four replications. Beet seeds, tomato and cucumber were submerged in chitosan solution for 20 minutes, in concentrations of 0.25, 0.5, 1 and 2% in the control and distilled water. Seeds were sown in trays containing Plantmax Florestalr substrate sterilized and inoculated with Fusarium sp., Rhizoctonia solani K¨unh and Pythium sp., respectively for the three cultures. The experiment was conducted for 14 days in growth chamber with controlled temperature (25 C 2 C), light (12 hour photoperiod) and humidity (70% 10%). The evaluations were seed emergency, seedling damping-off, seedling length, fresh weight and activity of the enzymes phenylalanine amˆonia-liase (PAL), chitinase and b-1,3-glucanase. It was also rated the mycelial growth of Fusarium sp., Pythium sp. and R. solani on P.D.A. (Potato-Dextrose and Agar) culture medium containing chitosan at the same concentrations evaluated in seeds. For beet growing, seed treatment with chitosan presented higher emergence and the length of the seedlings, and reduced the percentage of tipping. Treatment with chitosan activated the systemic acquired resistance with expression of chitinase and b-1,3-glucanase enzymes. For the tomato crop in chitosan concentration of 0.25% favored the emergency of seedlings, reduced the incidence of tipping and activated the PAL enzymes, chitinase and b-1,3-glucanase. In cucumber on the concentration of up 0.5% favored seedlings emergence and reduces the incidence of tipping. Chitosan activated the PAL enzymes and b-1,3-glucanase. Chitosan also presented fungistatic action on the initial growth of Pythium sp. and R. solani in vitro conditions, however, such action did not prevail until the end of the experiment. To Fusarium sp. the concentration of chitosan resulted in the reduction of mycelial growth in vitro.
Rankin, Lynda. "Evaluation of native rhizosphere bacteria for use as biological control agents against Pythium aphanidermatum root rot of European greenhouse cucumbers". Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56660.
Texto completoJung, M. C. Victoria. "The role of selected plant and microbial metabolites in the nutrient solution of closed growing systems in greenhouses /". Alnarp : Swedish University of Agricultural Sciences, 2003. http://diss-epsilon.slu.se/archive/00000343/.
Texto completoAppendix consists of reprints and manuscripts of five papers co-authored with others. Includes bibliographical references. Also partially available online in PDF format; online version lacks appendix.
Franco, André Andrade. "Resíduos de deltametrina, aplicada em diferentes formulações, em compartimentos da cultura do pepino (Cucumis sativus L.) tutorado e ação do inseticida no controle da broca-das-cucurbitáceas Diaphania nitidalis (Cramer, 1782) (Lepidoptera: Crambidae)". Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11146/tde-11112004-140048/.
Texto completoThe agriculture development, the modernization of cultivation means, trying to meet a demanding market, has been based on the use of agricultural supplies to assure a high quality and quantity production. Such crops demand the use of pesticides, whose residues, especially in fruit and vegetable production, raise worries about the consumers health and of the workers who need to return to the treated crop with these pesticides. The objectives of this study were: a) to study the behavior of deltamethrin residues applied in different formulations in fruits, leaves and soil; b) to evaluate the dislodgeable residues of the insecticide in leaves, as a starting point for occupational exposure studies; c) to correlate deltamethrin concentration in fruit with the pickleworm control and d) to correlate residues in fruit with the maximum residue level (MRL) and the safety interval established by the legislation. The treatments were: a) control ; b) three applications of the emulsifiable concentrate formulation Decis 25 CE.100-1 at the dosage of 30 mL of c.p.100 L-1 water (0.75 g a.i. deltamethrin.100L-1 water); c) three applications of the emulsifiable formulation of Decis Ultra 100 CE at the dosage of 7.5 mL of p.c.100 L-1 water (0.75 g a.i. deltamethrin.100. L-1 water) and d) three applications of the concentrate suspension formulation Decis 200 SC at the dosage of 3.75 mL of c.p.100.L-1 water (0.75 g a.i. deltamethrin 100L-1 water). The fruit and soil samples were taken at (-1); zero; 1; 3; 5 and 7 days after the last application; leaf samples and also those for the studies of dislodgeable residues on them were collected at (-1); zero; 1; 3; 5; 7 and 14 days after the last application. The analytical method consisted of the extraction of deltamethrin residues in ethyl acetate, clean-up of the extracts by gel permeation chromatography (GPC), with elutition made with a mixture of ethyl acetate/cyclohexane. The quantitative determination was made by gas chromatograph. To evaluate the insecticide efficacy six infestation surveys were carried out, based on the percentage of infested fruit . The results indicate that the deltamethrin residues in fruit, though in low levels, were found higher than the MRL (0.03 mg.kg-1), for the three insecticide formulations, even a day after the end of the safety interval (2 days). In the leaves the resulting residues of the applications of SC formulation were always bigger than both CE (10-20 times), being 15-80 times higher in the fruits. In the soil, the residues were also low (0.01-0.05 mg kg-1), and similar in the three formulations, however with low dissipation. The dislodgeable residues of deltamethrin in leaves were higher in SC as compared to both CE (4-20 times), with half-life values of 2.8; 3.8 and 32.2 days, respectively for the formulation Decis 25 CE, Decis Ultra 100 CE and Decis 200 SC. The insecticide in the formulations applied was efficient in the pest control during the whole evaluation period.
Estévez, Geffriaud Virginia. "Seed treatments for the protection of crops". Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/672956.
Texto completoJIAN, WEI-MING y 簡偉明. "Immunodetection of cucumber mosaic virus 3A protein expressed in plant". Thesis, 1992. http://ndltd.ncl.edu.tw/handle/72965204493554713940.
Texto completoCHANG, CHIN-YANG y 張晉揚. "The Construction and Evaluation of Cucumber (Cucumis sativus L.) under Plant Factory". Thesis, 2018. http://ndltd.ncl.edu.tw/handle/ffddd8.
Texto completo大葉大學
分子生物科技學系碩士班
106
Cucumber is one of the most economic vegetable worldwide. It also is one of the highest pesticide residues crops. For maintaining cucumber quality, high frequencies of pesticide spray were applied for cucumber cultivation. This study's aim was to produce the high quality cucumber with pesticide-free. Plant factory is a better approach to produce crops under correctly controlling plant growth conditions. The results showed that cucumber plants grew vigor under plant factory. By measuring and correcting pH and EC values, the hydroponic solutions was maintained optimal conditions for plant growing. The first fruit was harvested the thirty days after planting. We compared different thinning treatments on fruit quantity and quality. (1) 219 numbers of fruit and 28.2 Kg of total fresh weight were harvested three months after planting under control (non-thinning) treatment. (2) 529 numbers of fruit and 63 Kg of total fresh weight were harvested four months after planting under thinning treatment. (3) 143 numbers of fruit and 8.6 Kg of total fresh weight were harvested one month after planting under the special treatment. The radios of straight and curved fruit were 9:1 in special treatment, 7:3 in thinning treatment and 6:4 in control treatment, respectively. Finally, we alsocompared varied numbers of main shoot on yield. The results showed that tree numbers of main shoot was the best yield. Keywords:Plant factory, Cucumber, Yield assessment, Fruit quality
Hongtrakul, Tawatchai. "Attractivity of plant volatiles and a semichemical-based bait to the western spotted cucumber beetle, Diabrotica undecimpunctata Mannerheim (Coleoptera: Chrysomelidae)". Thesis, 1997. http://hdl.handle.net/1957/34002.
Texto completoGraduation date: 1998
Scherf, Andrea. "Licorice, cucumber, downy mildew: tracing the secret Interactions between the plant extract, the host and the pathogen". Phd thesis, 2012. https://tuprints.ulb.tu-darmstadt.de/2995/1/Disse_scherf_komplett_09.06.2012.pdf.
Texto completoSerce, Sedat. "The effect of plant habit and leaf size on water stress tolerance in cucumber (Cucumis sativus L.)". 1997. http://catalog.hathitrust.org/api/volumes/oclc/37751898.html.
Texto completoTypescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 53-63).
Ko, Yu-Tien y 蓋玉恬. "Effect of different calcium concentration and root temperature treatment on plant growth and yield of cucumber ‘Hsia Di’(Cucumis sativus L.)". Thesis, 2008. http://ndltd.ncl.edu.tw/handle/64154933824594346121.
Texto completo國立中興大學
園藝學系所
96
Experiments were conducted to study the effects of different root temperatures and calcium concentration on growth and physiological response of cumber ‘Hsia Di’(Cucumis sativus L.) grown in soilless culture. Different calcium concentration at 140 and 70 ppm in Yamasaki’s nutrition formulation and day/night temperature of 35/28℃ and 28/21℃ were tested in the experiment. Results indicated that both root and shoot dry weight of plants grown in 35/28℃ and 70 ppm of calcium decreased significantly as compared to those in lower temperature and higher calcium concentration. The Ca contents of plants in lower day/night temperature were observed less affected by nutrient solution with lower calcium concentration. The Ca contents of the lower leaves and upper leaves of the plants grown in 28/21℃ were 6.04 % and 2.21 % while those of plants in 35/28℃ were 4.4 % and 1.4 % , respectively. Both treatments of root temperature control at 26℃ or medium mulch mulching with PE film had the effects of increase above ground fresh weight and leaf area, and higher Ca contents in upper leaves and xylem exudates. Under lower temperature condition of 29/21℃, the Ca contents in xylem exudates were little affected by lowering the Ca concentration from 140 ppm to 70 ppm. However, those of plants in un-mulched and mulched media decreased 44 ppm and 89 ppm, respectively, when the Ca concentration supply were decreased from 140 ppm to 70 ppm. It is postulated that the control of root temperature at 26℃ enhanced the calcium absorption by roots the transport to above ground parts than did other treatments. Hence, focusing on more effective and economical methods to lower the medium temperature during the hot summer season will be the main aims in future study.
Basson, Jo-Anne. "The relative effectiveness of homeopathic preparations of pythium spp. compared to Previcur (Propamocarb), in the control of pythium root rot (damping off) in cabbage and cucumber seedlings". Thesis, 2002. http://hdl.handle.net/10321/2095.
Texto completoThe purpose of this study was to expand on the foundations laid by previous homeopathic research, using controlled agricultural experiments as an objective disease system. More specifically, these trials were to investigate and evaluate the efficacy of a fungicide (Previcur') against homeopathic preparations of a plant pathogenic fungus, Pythium, on seedlings infected with this fungus. Pythium is a worldwide problem and continues to cause substantial crop losses. Few chemical methods are available to control this disease and alternatives are being sought which are more cost-effective, safer for the environment and do not lead to pathogenic resistance. Relatively few trials using Homeopathy in plant pathology have been done to date. Furthermore, many of the earlier trials proved to have little scientific credibility, due to poor methods and insufficient data. More recent trials have shown interesting results, which have opened the doors for further investigation. This study compared the effects of Previcur\xAEagainst Homeopathic preparations of Pythium, on infected cabbage and cucumber seedlings. There were 28 trays for each crop and seven treatments per crop: four Homeopathic treatments (9CH, 15CH, 30CH and 200CH), two controls (one inoculated and the other un-inoculated) and Previcur'. The seedlings were treated twice weekly, using a 2.5ml drench per seedling, and were harvested three weeks after planting. The trial was run twice, in succession. Based on the nature of Pythium and the disease it causes, four parameters were measured in this trial. To assess the germination rate, the seedlings were counted three days after planting, to establish how many had broken the surface soil. To assess survival rate, the seedlings were counted at the time of harvesting. To assess growth, the seedlings were initially weighed wet (immediately after harvesting) and then dry (after 24 hours in an oven at 50\xB0C). The data was analysed by two methods: the General Linear Models Procedure (GLMP) produced ANOVA tables, which compared the variation between the four replicates of each of the seven treatments. The data was also analysed with the Student Newman Keuls (SNK) test, to determine the variation between the seven treatments. The results showed that Previcur®consistently produced the best or second best results, in terms of mean wet and dry weights, which was evident in the statistical analysis. However, a noticeable trend emerged with the 30CH treatment: it provided the lowest mean weights in seven of the eight parameters measured while showing evidence of consistent statistical significance from the other treatments throughout both trials. The implications were that this treatment had either enhanced the disease process or had suppressed plant growth. Student Newman Keuls (SNK) test, to determine the variation between the seven treatments. This trial raised issues regarding the effects of homeopathy on disease, as well as the internal mechanisms of disease control within plant structures. Further research into this area is encouraged, to further the platform already laid by statistically valid trials done thus far.
M
Shibata, Reiko. "The relationships between plant responses to arbuscular mycorrhizal fungi and phosphorus dynamics in the hyphae". 2007. http://hdl.handle.net/2440/57407.
Texto completoTwo experiments were conducted to find diversity in plant responses to arbuscular mycorrhizal (AM) fungi: positively and negatively/non-responsive combinations. Medic (Medicago truncatula L.) tomato (Solanum esculentum L.), leek (Allium porrum L.) and cucumber (Cucumis sativus L.) were inoculated with Glomus intraradices, Gigaspora margarita and Scutellospora calospora. It was found that soil differences critically affect plant responses to AM fungi.
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Thesis (Ph.D.) -- University of Adelaide, School of Earth and Environmental Sciences, 2007
Shibata, Reiko. "The relationships between plant responses to arbuscular mycorrhizal fungi and phosphorus dynamics in the hyphae". Thesis, 2007. http://hdl.handle.net/2440/57407.
Texto completoThesis (Ph.D.) -- University of Adelaide, School of Earth and Environmental Sciences, 2007
Sega, Davide. "FePO4 nanoparticles as a source of nutrients for plants: synthesis and evaluation of their effects on hydroponically grown cucumber and maize seedlings". Doctoral thesis, 2018. http://hdl.handle.net/11562/977172.
Texto completoCheng, Hao-Wen y 鄭浩文. "Development and application of epitope tags system derived from the plant-infecting Tospovirus and establishment of an in-vivo Cucumber green mottle mosaic virus vector". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/80123460471410021713.
Texto completo國立中興大學
植物病理學系所
102
The protein-protein interaction influences existence and behavior of the biological phenomenon. Epitope tagging is a technique that a known epitope is fused to recombinant proteins by genetic cloning. It is useful to characterize and monitor newly discovered proteins and existing proteins without the need for specific antibodies. One of the important applications of epitope-tagging is co-immunoprecipitaion. In this technique, a tagged-protein is immunoprecipitated with an anti-tag antibody to pull down proteins interacting directly with the tagged-protein. The power of this technique has contributed greatly to investigate protein-protein interactions involved in particular biological phenotypes. In recent years, abundant plant genome information has become available through genome sequencing analysis. Virus-induced gene silencing (VIGS) is a powerful and efficient tool to investigate gene functions in plants because of its rapidness and high-throughput. A recombinant virus for VIGS can deliver a fragment of plant gene into plant cells for inducing post-transcriptional gene silencing (PTGS) on the targeted endogenous gene to knock-down or abolish the related functions. Cucumber green mild mosaic virus (CGMMV) is a member of Tobamovirus, which infects cuburbit with high titers. The gene expression strategy of this virus makes it an ideal VIGS vector to be used for the cucurbitaceous plants. In this dissertation, Chapter 1 is the literature review describing the above relevant studies. Chapter 2 describes the development of an efficient tag derived from the common epitope of tospoviral NSs proteins for monitoring recombinant proteins expressed in both bacterial and plant systems. NSscon (23 aa), a common epitope in the gene silencing suppressor NSs proteins of the members of the Watermelon silver mottle virus (WSMoV) serogroup, was previously identified. In this investigation, green fluorescent protein (GFP)-fused with different deletions of NSscon were expressed in bacteria and reacted with NSscon monoclonal antibody (MAb). Our results indicated that the core 9 amino acids of NSscon, “109KFTMHNQIF117”, denoted as “nss”, retained the reactivity of NSscon. In bacterial pET system, four different recombinant proteins labeled with nss, either at N- or C-extremes, were readily detectable without position effects, with sensitivity superior to that for the polyhistidine-tag. When the nss-tagged Zucchini yellow mosaic virus (ZYMV) helper component-protease (HC-Pro) and WSMoV nucleocapsid protein (NP) were transiently expressed by agroinfiltration in tobacco, they were readily detectable and the tag’s possible efficacy for gene silencing suppression was not noticed. Co-immunoprecipitation of nss-tagged and non-tagged proteins expressed from bacteria confirmed the interaction of potyviral HC-Pro and coat protein. Thus, we conclude that this novel nss sequence is highly valuable for tagging recombinant proteins in both bacterial and plant expression systems. This chapter has been published in Journal of Biotechnology, 2013. Chapter 3 describes the identification of a conserved epitope of NP of Euro-America type tospoviruses and its application as an eitope tag for recombinant proteins. The antisera prepared against the abundant tospoviral NSs or NP in infected tissues have widely been used as the main serological tools for the detection of vital tospoviruses. The MAb 20C4C8, prepared in this study against TSWV NP, was found to be able to recognize most of the Euro-American type tospoviruses at a conserved epitope of NP. The MAb 20C4C8-recognizable short peptide “211KGKEYA216”, designated tnp, trimmed from the conserved epitope of TSWV NP was used for tagging recombinant proteins expressed in the bacterial system. Using MAb 20C4C8, the tnp-tag was further applied in co-immunoprecipitation for verifying the interaction between ZYMV CP and HC-Pro. Furthermore, bacteria expressed recombinant proteins were tagged and characterized with both nss-tag and tnp-sequence, and these two tags performed amicably. Taken together, the 6 amino acid tnp-tag (KGKEYA) can be efficiently applied in bacterial expression system for monitoring recombinant proteins. Also, the tnp-tag can be coupled with the nss-tag to form a novel epitope tag system for the study of protein-protein interactions. Chapter 4 describes the elucidation of the full-length sequence and development of a Taiwan strain of CGMMV as an in vivo plant virus vector. CGMMV is a cucurbit-infecting tobamovirus, with a genome of ss(+) RNA of 6423 nucleotides. The gene expression strategy of CGMMV makes it a good candidate to be constructed as a VIGS vector for cucurbitaceous plants. Following sequencing, the full-length cDNA of CGMMV was cloned into an Agrobacterium binary vector, driven by a Cauliflower mosaic virus (CaMV) 35S promoter and terminated by a Hepatitis delta virus (HDV) ribozyme sequence for generation of the exact 3'' end of CGMMV RNA. When the constructed full-length cDNA clone pk-CG was introduced into tobacco, horn melon, cucumber and watermelon plants by agroinfiltration, symptoms similar to those induced by the wild type virus were noticed and the infection was confirmed by ELISA with CGMMV antiserum. The transcription start site for the CP sub-genomic (sg) RNA was determined by 5'' RACE; the result indicated that the promoter is 13 bases upstream of the start codon of CP open reading frame (ORF). The pk-CG was mutated at CP ORF (+52) by site-direct mutagenesis to create a NcoI site for in-frame insertion of the GFP ORF. The constructed CGMMV recombinant carrying the fused GFP was introduced into tobacco plants by agroinfiltration and the expression of GFP was observed in the infiltrated leaves. However, the numbers of GFP expression foci were low. The CGMMV vector with different heterologous promoters derived from other tobamoviruses expressed GFP at higher levels in the infiltrated leaves, but systemic spread was not observed. In addition, the multiple cloning sites were also created between CP ORF and 3'' UTR for carrying foreign DNA fragment, but the recombinant was not stable. In conclusion, CGMMV vector is able to express GFP in the initially infected cells of infiltrated leaves and its systemic translocation remains to be further improved. Taken all together, the main purposes of this dissertation were directed to develop epitope tag system from the plant-infecting tospoviruses and to generate a cucurbit-infecting tobamovirus vector. Small epitope tags are useful to detect and analyze protein functions. The two small epitope tags from tospoviral proteins are immunodetectable with high specificity and they do not interfere with protein functions. Thus our results indicate that they are valuable for monitoring recombinant proteins. On the other hand, we have also developed a cucurbit-infecting CGMMV vector, even though the vector needs to be further improved for its efficacy as a VIGS inducer. Nevertheless, the CGMMV vector is still valuable for studying the plant-virus interaction, such as viral cell-to-cell movement in cucurbits. Finally, the two tospoviral epitope tags and the CGMMV vector can be further incorporated to increase their feasibility for many studies.
DOUL, Lukáš. "Škůdci rychlených okurek a rajčat - informační a výukový systém". Master's thesis, 2008. http://www.nusl.cz/ntk/nusl-48289.
Texto completoBallar��, Carlos L. "Photomorphogenic processes in the agricultural environment". Thesis, 1992. http://hdl.handle.net/1957/35773.
Texto completoHo, Hsiu-Yin y 何琇銀. "Development of transgenic watermelon plants with multiple resistance to Cucumber mosaic-、Cucumber green mottle mosaic- and Watermelon mosaic viruses". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/23150203013240623582.
Texto completo中興大學
農藝學系所
95
Watermelon is an important commercial crop in Taiwan and is prone to attack by several plant viruses. Previous studies have shown that transgenic plants with multiple viral gene fragments linked to an universal silencer DNA can provide resistance to multiple virus via posttranscriptional gene silencing (PTGS). In this study, similar approach was used to develop transgenic watermelon plants resistant to three different viruses via gene silencing. Transformation constructs with selection marker gene and targer gene either in the same T-DNA or in different T-DNAs containing a silencer DNA from the partial N gene of Watermelon silver mottle virus (WSMoV) fused to the partial coat protein genes of Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV) and Watermelon mosaic virus (WMV) were generated and used to transform watermelon plant via Agrobacterium-mediated transformation. A total of twenty-five trnasgenic lines were generated and confirmed by PCR amplification of selection marker gene. Southern blot was used for analyzing the copy number of the target gene inserted the genome of each transgenic watermelon plant. Transgenic watermelon plants were challenged with CMV, CGMMV, WMV or a mixture of these three viruses to evaluate the multiple virus resistance. Among the twenty-five tested lines, three lines showed resistant to CGMMV and WMV. Among these three resistant lines, two were also resistant to CMV and mixed inoculation of three viruses. The result of northern blotting of transgenic watermelon plants showed a correlation between the resistant phenotype and low accumulation level of target gene RNA transcripts. Our preliminary results indicate that transgenic watermelon carrying the fusion of different viral CP gene fragments can provide multiple virus resistance. R1 seeds from the self-pollinated resistant R0 plants were obtained and will be used to evaluate the broad-spectrum resistance to CMV, CGMMV, WMV and WSMoV.