Literatura académica sobre el tema "CTV"
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Artículos de revistas sobre el tema "CTV"
Powell, Charles A. y Youjian Lin. "Separation of Citrus Tristeza Virus Isolates in Mixed Infections through Transfer by Single Brown Citrus Aphids". HortScience 40, n.º 3 (junio de 2005): 694–96. http://dx.doi.org/10.21273/hortsci.40.3.694.
Texto completoLong, Amy, Francis LeBlanc, Jean-René Arseneau, Nellie Gagne, Katja Einer-Jensen, Jan Lovy, Mark Polinski, Simon Jones y Kyle A. Garver. "Distribution and Pathogenicity of Two Cutthroat Trout Virus (CTV) Genotypes in Canada". Viruses 13, n.º 9 (31 de agosto de 2021): 1730. http://dx.doi.org/10.3390/v13091730.
Texto completoWu, Fengnian, Mochi Huang, Eduardo G. P. Fox, Jiaquan Huang, Yijing Cen, Xiaoling Deng y Meirong Xu. "Preliminary Report on the Acquisition, Persistence, and Potential Transmission of Citrus tristeza virus by Diaphorina citri". Insects 12, n.º 8 (17 de agosto de 2021): 735. http://dx.doi.org/10.3390/insects12080735.
Texto completoBiswas, Kajal, Supratik Palchoudhury, Prosenjit Chakraborty, Utpal Bhattacharyya, Dilip Ghosh, Palash Debnath, Chandrika Ramadugu, Manjunath Keremane, Ravi Khetarpal y Richard Lee. "Codon Usage Bias Analysis of Citrus tristeza Virus: Higher Codon Adaptation to Citrus reticulata Host". Viruses 11, n.º 4 (8 de abril de 2019): 331. http://dx.doi.org/10.3390/v11040331.
Texto completoOkano, Takumi, Naoki Kobayashi, Kazuki Izawa, Tomoya Yoshinari y Yoshiko Sugita-Konishi. "Whole Genome Analysis Revealed the Genes Responsible for Citreoviridin Biosynthesis in Penicillium citreonigrum". Toxins 12, n.º 2 (15 de febrero de 2020): 125. http://dx.doi.org/10.3390/toxins12020125.
Texto completoRoy, Avijit, G. Ananthakrishnan, John S. Hartung y R. H. Brlansky. "Development and Application of a Multiplex Reverse-Transcription Polymerase Chain Reaction Assay for Screening a Global Collection of Citrus tristeza virus Isolates". Phytopathology® 100, n.º 10 (octubre de 2010): 1077–88. http://dx.doi.org/10.1094/phyto-04-10-0102.
Texto completoWallis, Christopher M., Zachary Gorman, Rachel Rattner, Subhas Hajeri y Raymond Yokomi. "Amino acid, sugar, phenolic, and terpenoid profiles are capable of distinguishing Citrus tristeza virus infection status in citrus cultivars: Grapefruit, lemon, mandarin, and sweet orange". PLOS ONE 17, n.º 5 (10 de mayo de 2022): e0268255. http://dx.doi.org/10.1371/journal.pone.0268255.
Texto completoFang, D. Q., C. T. Federici y M. L. Roose. "A High-Resolution Linkage Map of the Citrus Tristeza Virus Resistance Gene Region in Poncirus trifoliata (L.) Raf." Genetics 150, n.º 2 (1 de octubre de 1998): 883–90. http://dx.doi.org/10.1093/genetics/150.2.883.
Texto completoMaragos, Chris M., Yosuke Uchiyama, Naoki Kobayashi, Fumichika Kominato y Yoshiko Sugita-Konishi. "Development and Characterization of Monoclonal Antibodies for the Mycotoxin Citreoviridin". Toxins 11, n.º 11 (30 de octubre de 2019): 630. http://dx.doi.org/10.3390/toxins11110630.
Texto completoHughes, G. y T. R. Gottwald. "Survey Methods for Assessment of Citrus Tristeza Virus Incidence When Toxoptera citricida Is the Predominant Vector". Phytopathology® 89, n.º 6 (junio de 1999): 487–94. http://dx.doi.org/10.1094/phyto.1999.89.6.487.
Texto completoTesis sobre el tema "CTV"
Read, David Alan. "Overcoming bias in Citrus tristeza virus (CTV) genotype detection and a population study of CTV within Southern African Star Ruby grapefruit orchards". Thesis, University of Pretoria, 2015. http://hdl.handle.net/2263/53554.
Texto completoThesis (PhD)--University of Pretoria, 2015.
Microbiology and Plant Pathology
PhD
Unrestricted
Tsunoda, Fabio Silva. "Comissão Teotônio Vilela (CTV): direitos humanos e vocação militante". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/8/8132/tde-18042013-110243/.
Texto completoThe present work is an investigation about the process of professionalization of human rights defense in Brazil, considered through the case study of Comissão Teotônio Vilela (CTV). Founded in 1983, during the democratic transition, CTV started its work with the defense of regular prisoners and, with the democratic consolidation advancement, its claim agenda was either changed. In parallel, the trajectory of its members is also analyzed to show how they worked individually for the promotion and protection of human rights in Brazil. The research was conducted through interviews with founding members of the CTV, as well as the archives of the entity based at Center for the Study of Violence (NEV / USP), taking into account their reports, reports of visits, newspaper clippings and processes designed to seek some claim. The results suggest that the defense of human rights in Brazil was pervaded by two aspects, namely: increasing participation in state and governments and also in the internationalization process of claims.
Souza, Amancio José de. "Reação à infecção pelo vírus da tristeza dos citros (CTV) em plantas transgênicas de laranja \'Hamlin\' (Citrus sinensis (L.) Osbeck) expressando seqüências gênicas do CTV". Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-25072008-123421/.
Texto completoThe Citrus tristeza virus (CTV) is one of the greatest threats to the citrus industry worldwide. In Brazil, CTV continues to cause damage through strong strains despite the use of techniques like cross-protection and substitution of intolerant rootstocks. With the appearance and spread of the Citrus Sudden Death disease in 1999 and its possible relation to CTV, this virus was again among important pathogens within the Brazilian citrus industry. One of the possible solutions for controlling virus diseases in fruit crops is the development of immune or resistant transgenic plants. The objective of this work was to evaluate the resistance to CTV of transgenic \'Hamlin\' sweet orange plants containing three transgenic constructs obtained from CTV genomic sequences. The genetic constructs used aimed to activate RNAi defense routes (coat protein hairpin and a conserved sequence from CTV) and resistance mechanisms related to the coat protein expression. The transgenic plants were challenged with a weak strain of CTV, CTV-IAC, by bud and aphid (Toxoptera citricida Kirkaldy) inoculation. The evaluation of viral replication was done by ELISA analysis. The transgenic plants were considered susceptible to viral replication and translocation when bud inoculated. However, a few plants showed retardation of infection. It was not possible to determine resistance in the aphid transmission assay since the controls were not uniformly inoculated.
Hjulfors, Emmelie Maria. "Optimal margins between clinical target volume (CTV) and planning target volume (PTV)". Thesis, Umeå universitet, Institutionen för fysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-44824.
Texto completoGonçalves, Ana Claudia [UNESP]. "Separação de virus de importância fitopatológica em citros: CTV e CSDaV através de citometria de fluxo". Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/100738.
Texto completoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Devido a grande importância econômica da citricultura no Brasil e mundo e aos problemas sanitários enfrentados sendo alguns limitantes para o cultivo, como é o caso das doenças causadas por vírus como: a tristeza cítrica, causada pelo vírus da tristeza dos citros (CTV), pertencente ao gênero Closterovirus, família Closteroviridae, uma das maiores ameaças da citricultura mundial, mesmo com a pré imunização através de estirpes fracas do vírus e substituição de porta enxertos, estirpes fortes de CTV ainda causam prejuízos consideráveis. E com o aparecimento da doença morte súbita dos citros (MSC) de etiologia não determinada. Pelo fato de não haver ainda métodos eficientes de separação de ambos os vírus presentes em uma única amostra, levantando se as hipóteses que a causa da MSC esteja relacionada a uma estirpe do vírus CTV, a um vírus do gênero Marafivirus denominado Citrus Sudden Death-associated Virus (CSDaV), pertencente ao gênero Marafivirus, família Tymoviridae, ou a uma associação entre eles. Este trabalho vem propor um método eficaz de separação por citometria de fluxo (FC) de CTV e CSDaV em amostras semi purificadas, diluídas em tampão TE, pH7,5, utilizando marcação de ácidos nucléicos com Iodeto de Propídeo (PI) e conjugação de anticorpos policlonais anti CTV com Isotiocianato de Fluoresceína (FITC), cuja eficácia do método foi comprovada pela reação da polimerase em cadeia (PCR)
Because of high economic importance of citrus in Brazil and the world and health problems being faced some limiting factors for growing as is the case of diseases caused by viruses such as sadness citrus caused by citrus tristeza virus (CTV) belonging to Closterovirus gender, family Closteroviridae, one of the biggest threats to the citrus industry worldwide, even with the pre immunization using mild virus strains and replacement of the rootstocks, strong strains of CTV still cause considerable damage. And with the onset of the disease citrus sudden death (MSC) of undetermined etiology. Because there is not yet efficient methods of separation of the two viruses present in a single sample, raising the hypotheses that the cause of SCD is related to a strain of CTV, a virus Marafivirus group called Citrus Sudden Death-associated Virus (CSDaV) belonging to the genus Marafivirus, Tymoviridae family, or an association between them, this paper proposes an effective method of separation by flow cytometry (FC) and CTV in samples CSDaV semi purified, diluted in TE buffer, pH7, 5, using marking of nucleic acids with propidium iodide (PI) and a combination of polyclonal anti CTV with Fluorescein isothiocyanate (FITC), the effectiveness of the method was confirmed by polymerase reaction chain reaction (PCR)
Justino-Kuga, Elaine Aparecida. "Avaliação de epitopos na proteina do capsideo de isolados do virus da tristeza dos citros (CTV)". [s.n.], 1999. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314469.
Texto completoDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-07-25T23:46:58Z (GMT). No. of bitstreams: 1 Justino-Kuga_ElaineAparecida_M.pdf: 5500665 bytes, checksum: 5d07026a0dc49cbeb174c82abd0c0995 (MD5) Previous issue date: 1999
Resumo: Estudos preliminares sobre a localização de epítopos na proteína do capsídeo (CP) de três isolados de CTV ('Pêra IAC', 'Pêra CB-22' e 'Pêra CB-104') foram realizados em três etapas. Na primeira, foi realizada a amplificação de oito regiões distintas do gene da CP, codificantes para três regiões Nterminais (aa 1 até 70; aa 1 até 120; aa 1 até 170), três regiões C-terminais (aa 70 até 223; aa 120 até223; aa 170 até 223) e duas regiões internas da CP (aa 37 até 67 e aa 64 até 94) onde haviam sido determinadas diferenças significativas entre as CPs dos três isolados. Na segunda etapa, foram avaliadas duas estratégias para expressão dos peptídeos de interesse: clonagem em vetor de expressão através de ligação AT (vetor Pinpoint TM Xa-1 T, Promega) e clonagem das seqüências alvo em vetores do sistema de expressão pET. O vetor Pinpoint TM Xa-1 T, carreando como inserto o gene inteiro da CPCTV, obtido como produto de PCR após amplificação do cDNA dos três isolados, transformou células competentes de E. coli JM109, mas após indução com IPTG, não houve expressão da proteína de fusão esperada. Os vetores do sistema pET, após ligação das seqüências alvo, não transformaram células competentes de E. coli BL21(DE3)pLysS. Na terceira etapa, três proteínas recombinantes (MBP-CPCTV, CB-22 e CB-104) produzidas a partir da expressão do gene da CP-CTV dos três isolados, foram clivadas com brometo de cianogênio. Os produtos de clivagem foram avaliados através de '¿Western Blotting¿ contra anticorpos monoclonais específicos para CTV. O monoclonal IC-04.6, desenvolvido contra a proteína recombinante CB-22 detectou epítopos, numa reação intensa, em peptídeos com massa estimada em 27 kDa e 18 kDa, originários da CB-22; o monoclonal 39-08, desenvolvido contra a proteína recombinante CB-104 detectou epítopos, numa reação moderada, em peptídeos com massa estimada de 28 kDa e 14 kDa, originários qa CB-104; o monoclonal MCA-13, desenvolvido contra um isolado da Florida, detectou epítopos em peptídeos originários das proteínas recombinantes MBP-CPCTV e CB-104; o monoclonal 3DF1, desenvolvido contra isolados de CTV espanhóis, detectou epítopos em peptídeos originários da CB-22 e CB-104 e o monoclonal 3CA5, desenvolvido contra isolados espanhóis de CTV, detectou epítopos apenas num peptídeo com massa estimada de 18 kDa presente na CB-22. Estes resultados sugerem que diferentes epítopos são reconhecidos pelos cinco monoclonais avaliados e que serão necessárias novas estratégias para avaliá-los
Abstract: Preliminary studies about the epitopes location in the capside protein of the isolates of the citrus tristeza virus of three isolate ('Pêra IAC', 'Pêra CB-22' and 'Pêra CB-104') were accomplished. In the first phase, the amplification was accomplished with specific direct and reverse primers of eight different regions of the CP gene, coding for three N-terminal peptides (aa 1 to 70, aa 1 to 120 and aa 1 to 170), three C-terminal peptides (aa 70 to 223, aa 120 to 223 and aa 170 to 223) and two internal peptides of CP (aa 37 to 64 and aa 64 to 90). In the second phase they were appraised two strategies for expression of the peptides: cloning in expression vector through AT cloning site (vector Pinpoint TM Xa-1 T promega), and cloning of the coding sequences in vectors of the system pET. Vector Pinpoint TM Xa-1, containing as insert the whole gene of CP, obtained as PCR's product after amplification of the three isolates' cDNA, transformed competent cells E. coli JM109, but after induction with IPTG there was not expression of the peptides of interest. The vectors of the pET system didn't transform competent cells E. coli BL21 (DE3)pLysS. In the third phase, three recombinant proteins (MBP-CPC1V, CB-22 and CB-104), produced from the expression of the CP-C1V gene of the three isolates, they were broken with cyanogen bromide. The break products were evaluated through "Western Blotting" against monoclonal antibodies specific for CTV. The monoclonal IC-04.6, developed against the recombinant protein CB-22 detected epitopes, in an intense reaction, in peptides with mass esteemed in 27 kDa and 18 kDa, original of CB-22; the monoclonal 39-08, developed against the recombinant protein CB-104 detected epitopes, in a moderate reaction, in peptides with esteemed mass of 28 kDa and 14 kDa, original of CB-104; the monoclonal MCA-13, developed against the T-36 isolate from Florida, detected epitopes in original peptides of the recombinants proteins MBP-CPCTV and CB-104; the monoclonal 3DF1, developed against Spanish isolates of CTV, detected epitopes in original peptides of CB-22 and CB-104 and the monoclonal 3CA5, developed against Spanish isolates of C1V, just detected epitopes in a peptide with esteemed mass of 18 kDa present in CB-22. These results suggest that the five monoclonal recognizes different epitopes and that will be necessary new strategies to evaluate them
Mestrado
Bioquimica
Mestre em Biologia Funcional e Molecular
Gonçalves, Ana Claudia. "Separação de virus de importância fitopatológica em citros : CTV e CSDaV através de citometria de fluxo /". Araraquara, 2010. http://hdl.handle.net/11449/100738.
Texto completoBanca: Henrique Ferreira
Banca: Nelson Wulff
Banca: José Belasque Junior
Banca: Marcel Bellato Spósito
Resumo: Devido a grande importância econômica da citricultura no Brasil e mundo e aos problemas sanitários enfrentados sendo alguns limitantes para o cultivo, como é o caso das doenças causadas por vírus como: a tristeza cítrica, causada pelo vírus da tristeza dos citros (CTV), pertencente ao gênero Closterovirus, família Closteroviridae, uma das maiores ameaças da citricultura mundial, mesmo com a pré imunização através de estirpes fracas do vírus e substituição de porta enxertos, estirpes fortes de CTV ainda causam prejuízos consideráveis. E com o aparecimento da doença morte súbita dos citros (MSC) de etiologia não determinada. Pelo fato de não haver ainda métodos eficientes de separação de ambos os vírus presentes em uma única amostra, levantando se as hipóteses que a causa da MSC esteja relacionada a uma estirpe do vírus CTV, a um vírus do gênero Marafivirus denominado Citrus Sudden Death-associated Virus (CSDaV), pertencente ao gênero Marafivirus, família Tymoviridae, ou a uma associação entre eles. Este trabalho vem propor um método eficaz de separação por citometria de fluxo (FC) de CTV e CSDaV em amostras semi purificadas, diluídas em tampão TE, pH7,5, utilizando marcação de ácidos nucléicos com Iodeto de Propídeo (PI) e conjugação de anticorpos policlonais anti CTV com Isotiocianato de Fluoresceína (FITC), cuja eficácia do método foi comprovada pela reação da polimerase em cadeia (PCR)
Abstract: Because of high economic importance of citrus in Brazil and the world and health problems being faced some limiting factors for growing as is the case of diseases caused by viruses such as sadness citrus caused by citrus tristeza virus (CTV) belonging to Closterovirus gender, family Closteroviridae, one of the biggest threats to the citrus industry worldwide, even with the pre immunization using mild virus strains and replacement of the rootstocks, strong strains of CTV still cause considerable damage. And with the onset of the disease citrus sudden death (MSC) of undetermined etiology. Because there is not yet efficient methods of separation of the two viruses present in a single sample, raising the hypotheses that the cause of SCD is related to a strain of CTV, a virus Marafivirus group called Citrus Sudden Death-associated Virus (CSDaV) belonging to the genus Marafivirus, Tymoviridae family, or an association between them, this paper proposes an effective method of separation by flow cytometry (FC) and CTV in samples CSDaV semi purified, diluted in TE buffer, pH7, 5, using marking of nucleic acids with propidium iodide (PI) and a combination of polyclonal anti CTV with Fluorescein isothiocyanate (FITC), the effectiveness of the method was confirmed by polymerase reaction chain reaction (PCR)
Doutor
Moya, Gay Patricia. "Variabilidad genética y evolución del virus de la tristeza de los cítricos (CTV) en procesos de transmisión". Doctoral thesis, Universitat de València, 2010. http://hdl.handle.net/10803/41732.
Texto completoCTV isolates are composed of a population of sequence variants, resulting from mutation and recombination events. The frequency of these variants in the population is the outcome of different selective pressures, and migration and genetic drift phenomena generally associated to transmission processes. Here we analyzed separately the effect of different factors of the transmission process on the diversity and population structure of CTV isolates. We studied the effect of aphid transmission by nucleotide sequence comparisons between the donor and receptor plants and the collection CTV isolate. Transmission efficiency was low, and no sequence variation was observed. Aphid and graft transmission are a bottleneck resulting in a founder effect. We analysed the effect of graft transmission to a host of the same species. No obvious changes were observed in the CTV population structure and diversity We also studied if graft-transmission to different varieties propagated on Carrizo citrange could alter the CTV population. Although significant diversity changes were not observed, the population structure was occasionally altered due to the appearance of new sequence variants genetically close that became predominant in these populations. Finally, we used a clonal CTV isolate to study genetic variation generated de novo in different hosts. This isolate was serially passed by graft-transmission in different susceptible hosts and in a partially resistant host (sour orange). While CTV population was stable in the susceptible hosts, passages through sour orange caused major changes due to the appearance of new diverged sequence variants previously found in two natural isolates). Detection of minor variants phylogenetically located between these three genotypes supports the idea of an evolutionary process between the original and two new genotypes to get adapted to sour orange. Recombination events involving the three genotypes supports the presence of more than one genotype in infected sour orange. Inoculation from sour orange to susceptible hosts showed progressive loss of infectivity, due in part to the low virus titer in this host. CTV could neither be detected in Mexican lime propagated on these sour orange plants, suggesting that some host factor might also block CTV movement from sour orange to lime.
Navarro, López Josep Amadeu. "Estudio preliminar de las interacciones del virus de la tristeza de los cítricos (CTV) y su huésped". Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/90468.
Texto completoEl virus de la tristeza de los cítricos (CTV) es un closterovirus con un RNA genómico (gRNA) de ssRNA (+) y ¿20 Kb organizado en 12 pautas de lectura abierta (ORFs) que codifican al menos 17 proteínas, algunas de función desconocida. Las proteínas p25 y p27 forman parte de un grupo de genes implicados en el ensamblaje del virión, y se ha demostrado que p25 junto con p20 y p23 son supresores de silenciamiento génico en algunas especies de Nicotiana, y la última es también un determinante de patogénesis. CTV tiene una gama de huéspedes muy restringida y de forma natural sólo infecta el floema de algunas especies de cítricos. Por ello, para trabajar con este patosistema virus-huésped, se requiere un huésped experimental adecuado y un sistema genético eficiente de CTV. Durante los últimos años hemos desarrollado un sistema genético de CTV basado en la agroinfección sistémica de Nicotiana benthamiana, una especie no-natural de este virus, con clones infecciosos del genotipo T36. Dicha infección va acompañada de síntomas característicos, algunos de los cuales son similares a los que el virus induce en cítricos. La interacción CTV-N. benthamiana es muy variable y genotipo-dependiente, sólo algunos aislados se replican en esta especie y únicamente T36 la infecta sistémicamente. En este trabajo, abordamos la función de las proteínas p20 y p25 de tres aislados de CTV que difieren en sus características patogénicas. La expresión transitoria de las p20 y p25 fusionadas a proteínas fluorescentes reveló su idéntica localización subcelular en N. benthamiana y cítricos. La proteína p20 se localizó en el citosol y el núcleo celular en agregados amorfos asociados a regiones perinucleares e inclusiones nucleares puntuales. En cambio, la expresión de la p25 de T36, T318A y T385 de CTV reveló una localización diferencial. Mientras la de T36 y T385 fue nuclear, la de T318A fue citosólica. Un análisis detallado de las regiones implicadas en dicha localización, reveló la existencia de una señal de exportación nuclear NES rica en leucinas en la región Nt de la proteína. Un análisis de la capacidad patogénica de p20 y p25 en N. benthamiana en un contexto de infección viral heterólogo a través de PVX, mostró que p25 no es un determinante de patogenicidad en esta especie, pero p20 sí. Por otra parte, el interactoma de la p25-T36 resultó mucho más complejo y diverso que el de la p25-T318A, interviniendo potencialmente en mayor número de procesos metabólicos de fotosíntesis, actividad redox, homeóstasis y transporte celular, biosíntesis y degradación de proteínas, unión a proteínas de los plastidios y ácidos nucleicos o de respuesta a estrés (biótico y oxidativo) o defensa mediada por la ruta del jasmónico, del ciclo de metilación, señalización por ROS y proteínas HSP. Las interacciones mayoritarias de la p25-T318A se relacionaron con transporte/localización y respuesta a estrés, principalmente con interactores implicados en procesos de apoptosis, patogénenis y proteínas HSP, de unión a calcio o redoxinas. También hemos conseguido la evolución experimental de CTV por pases seriados en N. benthamiana. Dicha evolución conlleva un conjunto de características adaptativas significativas como: el aumento del prendimiento de injertos, de la tasa neta de infectividad, del título viral y del adelanto de los síntomas causados en esta especie herbácea con el aumento de los pases. Las características adaptativas también se reflejaron a nivel molecular en la variabilidad genética y estructura de las poblaciones de los virus evolucionados en dos linajes independientes. Virus evolucionados en N. benthamiana resultaron menos infecciosos inicialmente por inoculación mecánica de regreso a cítricos, y se acumularon menos que el virus parental durante el primer año. Dicha re-adaptación de los virus evolucionados se reflejó a nivel molecular en la pérdida progresiva de las m
El virus de la tristesa dels cítrics (CTV) és un closterovirus amb un RNA genòmic de ssRNA(+) i 20 Kb organitzat en 12 pautes de lectura oberta (ORFs) que codifiquen, al menys, 17 proteïnes, algunes de les quals de funció desconeguda. Les proteïnes p25 i p27 formen part d'un grup de gens implicat en l'assemblatge del virió, i s'ha demostrat que p25, junt a p20 i p23, són supressors de silenciament gènic en algunes espècies de Nicotiana, i la última també és un determinant de patogènesi. La gama d'hostes de CTV és molt restringida i de forma natural sols infecta el floema d'algunes espècies de cítrics. Per això, per a treballar amb aquest patosistema virus-hoste, es requereix un hoste experimental adequat i un sistema genètic eficient de CTV. Durant els últims anys hem desenvolupat un sistema genètic de CTV basat en l'agroinfecció sistèmica de Nicotiana benthamiana, una espècie no-natural d'aquest virus, amb clons infecciosos del genotip T36. Aquesta infecció va acompanyada de símptomes característics, alguns dels quals són similars als que el virus indueix en cítrics. La interacció CTV-N. benthamiana és molt variable i genotip depenent, ja que sols alguns aïllats es repliquen en aquesta espècie i únicament T36 la infecta sistèmicament. En aquest treball hem abordat la funció de les proteïnes p20 i p25 de tres aïllats de CTV que difereixen en les seues característiques patogèniques. L'expressió transitòria de les p20 i p25 fusionades a proteïnes fluorescents va revelar la seua idèntica localització subcel·lular en N. benthamiana i cítrics. La proteïna p20 dels aïllats T36, T318A i T385 es va localitzar al citosol i al nucli cel·lular formant agregats amorfs associats a regions perinuclears, i inclusions nuclears puntuals. En canvi, l'expressió de la p25 de T36, T318A i T385 de CTV va revelar una localització diferencial. Mentre la de T36 i T385 fou nuclear, la de T318A fou citosòlica. Una anàlisi detallada de les regions implicades en eixa localització va revelar l'existència d'una senyal d'exportació nuclear (NES) rica en leucines a la regió Nt de la proteïna. L'anàlisi de la capacitat patogènica de p20 i p25 en N. benthamiana en un context d'infecció viral heteròloga a través de PVX, va mostrar que p25 no és un determinant de patogenicitat en aquesta espècie, però p20 sí. D'altra banda, l'interactoma de p25-T36 va resultar molt més complex i divers que el de p25-T318A, intervenint potencialment en un major nombre de processos metabòlics de fotosíntesi, activitat redox, homeòstasi i transport cel·lular, biosíntesi i degradació de proteïnes, unió a proteïnes dels plastidis i àcids nucleics o de resposta a estrés (biòtic i oxidatiu) o defensa mediada per la ruta del jasmònic, del cicle de metilació, senyalització per ROS i proteïnes HSP. Les interaccions majoritàries de la p25-T318A es relacionaren amb transport/localització i resposta a estrés, principalment amb interactors implicats en processos d'apoptosi, patogènesi i proteïnes HSP, d'unió a calci o redoxines. També hem aconseguit l'evolució experimental de CTV per passes seriats en N. benthamiana. Aquesta evolució comporta un conjunt de característiques adaptatives significatives com: l'augment de la supervivència dels empelts, de la taxa neta d'infectivitat, de la càrrega viral i de l'ajornament dels símptomes causats en aquesta espècie herbàcia amb l'augment dels passes. Les característiques adaptatives també es reflectiren a nivell molecular amb la variabilitat genètica i estructura de les poblacions dels virus evolucionats en dos llinatges independents. Virus evolucionats a N. benthamiana resultaren menys infecciosos inicialment per inoculació mecànica de tornada a cítrics, i s'acumularen menys que el virus parental durant el primer any. Aquesta re-adaptació dels virus evolucionats a N. benthamiana de tornada a cítrics es va reflectir a nivell molecular amb
Navarro López, JA. (2017). Estudio preliminar de las interacciones del virus de la tristeza de los cítricos (CTV) y su huésped [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/90468
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Kahlon, Amandeep Singh. "Molecular characterization of the population diversity of selected isolates and subisolates of Citrus tristeza virus (CTV) from Florida". [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0011870.
Texto completoLibros sobre el tema "CTV"
Gittins, Susan. CTV: The television wars. North York, Ont: Stoddart, 1999.
Buscar texto completoMichael, Nolan. CTV, the network that means business. Edmonton: University of Alberta Press, 2001.
Buscar texto completoConfederación de Trabajadores de Venezuela. CTV, 50 años de historia, 1936-1986. [Caracas?]: Confederación de Trabajadores de Venezuela, 1986.
Buscar texto completoChiappa, Ernestino. CTV: Il corpo truppe volontarie italiane durante la guerra civile spagnola, 1936- 1939 : cronistoria-uniformi. Milano: EMI, 2003.
Buscar texto completoC.V. Yerushalayim: Karmel, 2007.
Buscar texto completoCCTV. 3a ed. Boston: Butterworth-Heinemann, 2000.
Buscar texto completoMangum, L. J. CTD/Ob2s. Seattle, Wash: U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Environmental Research Laboratories, Pacific Marine Environmental Laboratory, 1985.
Buscar texto completoLynch, J. M. CTD/O. Seattle, Wash: U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Environmental Research Laboratories, Pacific Marine Environmental Laboratory, 1988.
Buscar texto completoLynch, J. M. CTD/Ob2s. Seattle, Wash: U.S. Dept. of Commerce, National Oceanic and Atmospheric Administration, Environmental Research Laboratories, Pacific Marine Environmental Laboratory, 1988.
Buscar texto completoMéxico, Confederación de Trabajadores de. Constitución CTM. México: Secretaría del Trabajo y Previsión Social, Subsecretaría "B", Unidad Coordinadora de Políticas, Estudios y Estadísticas del Tabajo, 1987.
Buscar texto completoCapítulos de libros sobre el tema "CTV"
Kim, Denise S., Remy R. Lobo y Alon Kahana. "Orbital CTA/CTV". En Atlas of Orbital Imaging, 1–4. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-41927-1_86-1.
Texto completoKim, Denise S., Remy R. Lobo y Alon Kahana. "Orbital CTA/CTV". En Atlas of Orbital Imaging, 113–16. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62426-2_86.
Texto completoKnowlton, Christin A., Michelle Kolton Mackay, Tod W. Speer, Robyn B. Vera, Douglas W. Arthur, David E. Wazer, Rachelle Lanciano et al. "Clinical Target Volume (CTV)". En Encyclopedia of Radiation Oncology, 125. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-540-85516-3_298.
Texto completoRusso, Marcella y Antonino F. Catara. "Phenotyping Biological Properties of CTV Isolates". En Methods in Molecular Biology, 15–27. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9558-5_3.
Texto completoEl Attar, Abderrahim, Mostafa El Hachloufi y Zine El Abidine Guennoun. "Optimal Reinsurance Under CTV Risk Measure". En Innovations in Smart Cities and Applications, 919–30. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-74500-8_82.
Texto completoSoler, Nuria, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores y Leandro Peña. "Methods for Producing Transgenic Plants Resistant to CTV". En Methods in Molecular Biology, 229–43. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9558-5_17.
Texto completoRodrigo, Javier. "Italy, the CTV, and politics on the National side". En Fascist Italy in the Spanish Civil War, 1936–1939, 102–32. Abingdon, Oxon ; New York, NY : Routledge, 2021. | Series: [Routledge/Cañada Blanch studies on contemporary Spain]: Routledge, 2021. http://dx.doi.org/10.4324/9781003166054-4.
Texto completoYokomi, Raymond. "CTV Vectors and Interactions with the Virus and Host Plants". En Methods in Molecular Biology, 29–53. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9558-5_4.
Texto completoDavis, R. Eugene, Gerard Duvierre, Yves Boussant-Roux y Michael Nelson. "High-Zirconia Fused Cast Refractory Applications in CTV Panel Glass Melters". En A Collection of Papers Presented at the 61st Conference on Glass Problems: Ceramic Engineering and Science Proceedings, Volume 22, Issue 1, 117–23. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2008. http://dx.doi.org/10.1002/9780470294659.ch10.
Texto completoScalliet, P., L. Renard, B. Lengelé y B. Tombal. "CTV for Lymphatics in Prostate Adenocarcinoma: an Anatomical Description and Clinical Discussion". En Clinical Target Volumes in Conformal and Intensity Modulated Radiation Therapy, 145–56. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-06270-8_7.
Texto completoActas de conferencias sobre el tema "CTV"
Perrier, P. y G. Durand. "CRV-CTV aerodynamic shape definition". En 8th AIAA International Space Planes and Hypersonic Systems and Technologies Conference. Reston, Virigina: American Institute of Aeronautics and Astronautics, 1998. http://dx.doi.org/10.2514/6.1998-1628.
Texto completoPotylitsyn, N. P., A. V. Britkov, Y. A. Krasyuk, N. A. Gorbanov y V. V. Zayanchukovskiy. "CTV optical equipment". En 2005 15th International Crimean Conference Microwave and Telecommunication Technology. IEEE, 2005. http://dx.doi.org/10.1109/crmico.2005.1564825.
Texto completoKrasyuk, Y. A., N. P. Potylitsyn, P. A. Rakityanskiy, S. A. Dementenko y A. E. Prokopenko. "Optical assembly for CTV". En 2004 14th International Crimean Conference "Microwave and Telecommunication Technology". IEEE, 2004. http://dx.doi.org/10.1109/crmico.2004.183200.
Texto completo"General background: history of CTV". En Virtual City and Territory. Barcelona: Centre de Política de Sòl i Valoracions, 2016. http://dx.doi.org/10.5821/ctv.8110.
Texto completoMorozov, A. A., A. V. Prokhorenko, V. N. Dyachenko, A. V. Britkov, V. V. Zayanchukovskiy, V. I. Sviridenko, N. A. Gorbanov et al. "A multifunctional CTV measuring device". En 2003 13th International Crimean Conference 'Microwave and Telecommunication Technology' Conference Proceedings. IEEE, 2003. http://dx.doi.org/10.1109/crmico.2003.158744.
Texto completoBritkov, A. V., A. V. Prohorenko, V. N. Dyachenko, D. N. Trembach, V. I. Sviridenko, N. A. Gorbanov y A. S. Nosov. "Multifunctional measuring device for CTV". En 2004 14th International Crimean Conference "Microwave and Telecommunication Technology". IEEE, 2004. http://dx.doi.org/10.1109/crmico.2004.183366.
Texto completoKinney, David, Jeff Bowles, Lily Yang y Cathy Roberts. "Conceptual design of a 'SHARP' - CTV". En 35th AIAA Thermophysics Conference. Reston, Virigina: American Institute of Aeronautics and Astronautics, 2001. http://dx.doi.org/10.2514/6.2001-2887.
Texto completoBritkov, A. V., N. A. Gorbanov, V. I. Sviridenko, O. S. Nosov, V. T. Gontarev, M. A. Tarasov, A. V. Prohorenko y V. N. Dyachenko. "LFM-700 multifunctional measuring device for CTV". En 2005 15th International Crimean Conference Microwave and Telecommunication Technology. IEEE, 2005. http://dx.doi.org/10.1109/crmico.2005.1565133.
Texto completoHerber, Nikolaus y Joachim Lucas. "The ECLS Subsystem for the European Crew Transfer Vehicle (CTV)". En International Conference On Environmental Systems. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 1996. http://dx.doi.org/10.4271/961373.
Texto completoChoudhary, Amit Kumar. "The impact of tumour regression in locally advanced carcinoma cervix during external beam radiotherapy and the need for adaptive planning". En 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685254.
Texto completoInformes sobre el tema "CTV"
Dawson, William O., Moshe Bar-Joseph, Charles L. Niblett, Ron Gafny, Richard F. Lee y Munir Mawassi. Citrus Tristeza Virus: Molecular Approaches to Cross Protection. United States Department of Agriculture, enero de 1994. http://dx.doi.org/10.32747/1994.7570551.bard.
Texto completoLee, Richard, Moshe Bar-Joseph, K. S. Derrick, Aliza Vardi, Roland Brlansky, Yuval Eshdat y Charles Powell. Production of Antibodies to Citrus Tristeza Virus in Transgenic Citrus. United States Department of Agriculture, septiembre de 1995. http://dx.doi.org/10.32747/1995.7613018.bard.
Texto completoBar-Joseph, Moshe, William O. Dawson y Munir Mawassi. Role of Defective RNAs in Citrus Tristeza Virus Diseases. United States Department of Agriculture, septiembre de 2000. http://dx.doi.org/10.32747/2000.7575279.bard.
Texto completoDawson, William O. y Moshe Bar-Joseph. Creating an Ally from an Adversary: Genetic Manipulation of Citrus Tristeza. United States Department of Agriculture, enero de 2004. http://dx.doi.org/10.32747/2004.7586540.bard.
Texto completoYin, Xietian, Shichao Zhao, Nan Xiang, Jidong Chen, Jun Xu y Yudan Zhang. Efficacy and Safety of Chinese Herbal Medicines combined with Cyclophosphamide for Connective Tissue Disease-Associated Interstitial Lung Disease: A Meta-Analysis of Randomized Controlled Trials. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, diciembre de 2022. http://dx.doi.org/10.37766/inplasy2022.12.0010.
Texto completoSchat, Karel Antoni, Irit Davidson y Dan Heller. Chicken infectious anemia virus: immunosuppression, transmission and impact on other diseases. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7695591.bard.
Texto completoChejanovsky, Nor y Bruce A. Webb. Potentiation of pest control by insect immunosuppression. United States Department of Agriculture, julio de 2004. http://dx.doi.org/10.32747/2004.7587236.bard.
Texto completoLeonard, Talayna, Robert Lemme, Cati Kral, Briana Santiago, Chris Elberts, Stephanie Dewald, Patrick McGonagill et al. High-Percentage of Early Resectable Pancreatic Ductal Adenocarcinoma is Unidentified on Abdominal CT Obtained for Unrelated Diagnosis. Science Repository, diciembre de 2021. http://dx.doi.org/10.31487/j.aco.2021.02.03.
Texto completoSkone, Timothy J. CTL Diesel. Office of Scientific and Technical Information (OSTI), agosto de 2013. http://dx.doi.org/10.2172/1509366.
Texto completoMcCaffrey, Trevor y Gordon T. Richards. CIV Distance. GitHub, octubre de 2021. http://dx.doi.org/10.17918/civdistance.
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