Literatura académica sobre el tema "CSF single chain library"
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Artículos de revistas sobre el tema "CSF single chain library"
Tapryal, Suman, Yogender Pal khasa y K. J. Mukherjee. "Single chain Fv fragment specific for human GM-CSF: Selection and expression using a bacterial expression library". Biotechnology Journal 5, n.º 10 (3 de septiembre de 2010): 1078–89. http://dx.doi.org/10.1002/biot.201000043.
Texto completoPiper, Clinton, Achia Khatun, Yao Chen, Ryan Zander, Weiguo Cui y William R. Drobyski. "Single Cell Immune Profiling of Colitogenic T Cells during Acute Graft Versus Host Disease Reveals Two Novel Transcriptionally Distinct CD4+ GM-CSF+ Lineages". Blood 134, Supplement_1 (13 de noviembre de 2019): 197. http://dx.doi.org/10.1182/blood-2019-127095.
Texto completoNing, Liangxia y Bin Wang. "Neurofilament light chain in blood as a diagnostic and predictive biomarker for multiple sclerosis: A systematic review and meta-analysis". PLOS ONE 17, n.º 9 (14 de septiembre de 2022): e0274565. http://dx.doi.org/10.1371/journal.pone.0274565.
Texto completoTashiro, Haruko, Ryosuke Shirasaki, Yoko Oka, Tadashi Yamamoto, Nobu Akiyama, Kazuo Kawasugi y Naoki Shirafuji. "Interleukin-1β Promotes the Expression of CD34 and Granulocyte Colony-Stimulating Factor-Receptor in Adult Dermal Fibroblasts". Blood 118, n.º 21 (18 de noviembre de 2011): 4815. http://dx.doi.org/10.1182/blood.v118.21.4815.4815.
Texto completoSmith, Eric L., Sham Mailankody, Arnab Ghosh, Reed Masakayan, Mette Staehr, Terence J. Purdon, Elizabeth Halton et al. "Development and Evaluation of a Human Single Chain Variable Fragment (scFv) Derived Bcma Targeted CAR T Cell Vector Leads to a High Objective Response Rate in Patients with Advanced MM". Blood 130, Suppl_1 (7 de diciembre de 2017): 742. http://dx.doi.org/10.1182/blood.v130.suppl_1.742.742.
Texto completoShort, M. K., T. Housel y intro by P. T. Jubinsky. "Selection of single chain antibody fragments against the gm-csf receptor". Experimental Hematology 28, n.º 7 (julio de 2000): 86. http://dx.doi.org/10.1016/s0301-472x(00)00355-6.
Texto completoHanna, Rachel, Lia Cardarelli, Nish Patel, Levi L. Blazer, Jarrett J. Adams y Sachdev S. Sidhu. "A phage‐displayed single‐chain Fab library optimized for rapid production of single‐chain IgGs". Protein Science 29, n.º 10 (15 de septiembre de 2020): 2075–84. http://dx.doi.org/10.1002/pro.3931.
Texto completoSchneider, Ruth, Barbara Bellenberg, Barbara Gisevius, Sarah Hirschberg, Roman Sankowski, Marco Prinz, Ralf Gold, Carsten Lukas y Aiden Haghikia. "Chitinase 3–like 1 and neurofilament light chain in CSF and CNS atrophy in MS". Neurology - Neuroimmunology Neuroinflammation 8, n.º 1 (10 de noviembre de 2020): e906. http://dx.doi.org/10.1212/nxi.0000000000000906.
Texto completoKamiya, Toshio, Osamu Saitoh y Hiroyasu Nakata. "Functional Expression of Single-Chain Heterodimeric G-Protein-Coupled Receptors for Adenosine and Dopamine". Cell Structure and Function 29, n.º 5,6 (2005): 139–45. http://dx.doi.org/10.1247/csf.29.139.
Texto completoRojas, Julio C., Jee Bang, Iryna V. Lobach, Richard M. Tsai, Gil D. Rabinovici, Bruce L. Miller y Adam L. Boxer. "CSF neurofilament light chain and phosphorylated tau 181 predict disease progression in PSP". Neurology 90, n.º 4 (27 de diciembre de 2017): e273-e281. http://dx.doi.org/10.1212/wnl.0000000000004859.
Texto completoTesis sobre el tema "CSF single chain library"
Cortini, Andrea. "Serum profiling and autoantibodies identification in Multiple Sclerosis using epitope and CSF IgG phage display libraries". Doctoral thesis, Università degli studi di Trieste, 2009. http://hdl.handle.net/10077/3073.
Texto completoMultiple sclerosis (MS) is considered the prototype of inflammatory autoimmune diseases of the central nervous system (CNS). The typical feature of the disease is the plaques of demyelination. The evolution of the plaque lesion in MS implicates an inflammatory phase followed by a recovery of functional myelin; a second step is the chronic progressive disease with axonal loss. The earlier phase of MS may be mediated by an autoimmune reaction. Whereas the role of T cells in MS pathogenesis is well established, the role of B cells and autoantibodies in demyelination and plaque formation is still unresolved. However several evidences suggest a contribute of autoantibodies in MS pathogenesis. B cells and myelin specific autoantibodies are present in the sclerosis plaques, and there is an increased production of immunoglobulin (Ig) in the cerebrospinal fluid (CSF) of more than 90% of MS patients . Typically these Ig present an oligoclonal pattern and sequencing of oligoclonal IgG showed extensive somatic mutations suggesting B cell clonal expansion and a specific antigen-driven immune response. The most extensively studied putative autoantigens are components of CNS myelin (myelin basic protein MBP, proteolipid protein PLP, myelin oligodendrocyte glycoprotein MOG). The autoantibodies in MS recognize both linear and conformational epitopes, but at present the conformational epitopes of myelin proteins have not been identified. For example, in MS, the T-cell receptors of autoreactive T lymphocytes recognize various peptides of the MBP, and, in EAE, the anti-MOG antibodies recognize only conformational epitopes. Furthermore, the progression of MS is accompanied by the decline of primary T-cell autoreactivity and by the concurrent emergence of neo-autoreactivity (epitope spreading). However recent investigation have showed that no myelin antigens, like neuron-specific enolase (NSE), retinal arrestin, beta-arrestin, may also have a role in MS pathogenesis. Autoimmunity against these antigens may be linked to neurodegeneration, defective remyelination, and predisposition to uveitis in multiple sclerosis. Several strategies, involving the phage display technology, have been employed in the attempt to discover the antigen that drives the immune response in MS. A first strategy depends on the cloning of IgG repertoire of MS patients in a phage display library screened with brain sections or known antigens. Another strategy involves large phage display libraries of random peptides screened with IgG of CSF in order to identify peptides recognized by antibodies present in CSF of MS patients. Phage display is a technique which involves the coupling of phenotype to genotype in a selectable format. It has been extensively used in molecular biology to study protein-protein interactions and to select antibodies against a wide range of different antigens. In this project we have proposed: 1. to study the autoimmune response in MS by using the phage display for the expression of antibodies involved in the disease. We wanted to make a ScFv library from B cells of CSF of different multiple sclerosis patients, to employ as tool to select a phage display Human Brain cDNA library for the identification of new antigens recognized by the immune sistem in MS patients. 2. To produce single gene mini library of putative antigens (MBP,PLP, MOG) for the generation of epitope chips to use for serotyping the immune response in different patients 3. To investigate the feasibility to use a single gene phage display mini-library as tool for epitope mapping (both linear and conformational) of novels autoantigens 4. To investigate the role of NSE(neuron specific enolase), a new possible no myelin autoantigen in multiple sclerosis, in the pathogenesis of the disease and the usefulness as possible diagnostic marker. Results: Scope 1 B-cells from liquor of two MS patients were centrifuged and the total RNA was extracted from the pellets. Total RNA was retrotranscripted and variable region of heavy and light chain of the antibodies were amplified by PCR. Heavy chain and light chain were assorted and assembled before to be cloned in the phagemid vector pDAN 5. A 2x104 independent clones library was obtained and analyzed by PCR and fingerprinting. A diversity of 30,8% for heavy chain and 72,7% for light chain was established. ScFv library was used to select a phage display Human Brain cDNA library. 17 clones with an high reactivity were obtained and after sequencing 6 clones on 17 have shown to be the same antigen(antigen A ); the reactivity on other two antigens obtained with the selection (antigen B and C) of CSF from 18 MS patients and 16 patients with other neurological disease (OND) was tested by ELISA to evaluate diagnostic value of this protein. The results shown that SM response was statistically different from OND response; the ELISA test gave a specificity of 94,12% and a significance of 53,85 %. The reactivity for the antigen B was also evaluated on sera of MS patients and controls. The MS response was statistically different from OND response and shown a specificity of 97,44% and a significance of 58,62 %. Scope 2&3 We have generated three single gene mini libraries of the major antigens in MS (MBP, MOG and PLP); cDNA of each gene was obtained by RT-PCR and after fragmentation cloned in a phagemid vector (pEP1) to obtain a mini-library for each gene. We have obtained a 2x105 for MBP, 2.4x104 for MOG and 1.6x106 for PLP independent clones library. MBP and MOG libraries were characterized by PCR and fingerprinting. Sequencing analysis shown that the entire MBP transcript variant 7 mRNA (664-1177 nt) and MOG isoform alpha 1 mRNA (262-918 nt) were represented in the respective library. To testing the capacity of selecting a single epitope from our libraries, we have performed a selection test with a commercial monoclonal antibody that recognize MBP 82-98 epitope; after three selection panning all selected clones contain the nucleotidic sequence 906- 956 nt (MBP transcript variant 7 mRNA) which encodes the immunogenic epitope recognized by the monoclonal antibody. Scope 4 The reactivity of sera from 31 MS patients and 14 healthy controls was tested by ELISA on NSE ; statistical analysis of the results shown that the two populations were significantly different.
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O'Brien, Siobhan Helen. "A single chain antibody bacteriophage display library from a patient with active uveoretinitis". Thesis, University of Aberdeen, 1999. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU123996.
Texto completoBosompem, Amma N. "Isolation of an anti-CD20 single chain variable fragment from a naïve human phage-scFv library". Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1202410076/.
Texto completoRoberts, Anthony Simon. "The cloning, characterisation and engineering of an IGF-I-BINDING single chain Fv". Thesis, Queensland University of Technology, 2004. https://eprints.qut.edu.au/15914/1/Anthony_Roberts_Thesis.pdf.
Texto completoRoberts, Anthony Simon. "The cloning, characterisation and engineering of an IGF-I-BINDING single chain Fv". Queensland University of Technology, 2004. http://eprints.qut.edu.au/15914/.
Texto completoPilger, Franziska [Verfasser]. "Construction of an equine antibody library in the single-chain-Fragment-variable format (scFv) to express equine immunoglobulins / Franziska Pilger". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://d-nb.info/1230824510/34.
Texto completoLi, Weiyi. "Protein Engineering Hydrophobic Core Residues of Computationally Designed Protein G and Single-Chain Rop: Investigating the Relationship between Protein Primary structure and Protein Stability through High-Throughput Approaches". The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1398956266.
Texto completoNdlovu, Siphumelele. "The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana". Master's thesis, Faculty of Science, 2020. http://hdl.handle.net/11427/32303.
Texto completoShahsavarian, Melody. "Genesis of immune diversity and selection of catalytic antibodies : a new investigation". Thesis, Compiègne, 2015. http://www.theses.fr/2015COMP2215/document.
Texto completoCatalytic antibodies (or abzymes) have been the focus numerous studies for some decades and have been produced with the ability to catalyze a wide range of reactions. They have also been discovered naturally in normal physiological and pathological conditions, notably on the background of autoimmune disease. Some have beneficial effects and others are detrimental to individual’s health. Hence, the origin of abzymes and their role in the immune response are ambiguous and must be enhanced. We have developed a novel strategy for the study of abzymes based on the phage display technology. We have constructed 4 libraries representing 4 murine immune repertoires with different genetic backgrounds and immunological states : healthy and naïve, healthy and immunized, autoimmune and naïve , and autoimmune and immunized. The strategies for the amplification and cloning of the immunoglobulin (lg) variable regions have been designed to optimize the size and diversity of the libraries. We have been able to pool the four libraries to create a large repertoire of size 2.7x109. After sequence analysis, we have found a number of statistically significant differences between the libraries. We have then used two strategically chosen targets to select for antibodies endowed with β lactamase activity : a cycle peptide and a penam sulfone, both inhibitors of the enzyme. We have selected for a total of 5 lgs endowed with β lactamase activity. The selected abzymes have different amino acid sequences. 3D modeling has provides insights on potential active sites demonstrating the ability of different structures to maintain the β lactamase activity and confirming the flexibility of the active site
"Construction and Characterization of a Single-Chain Variable Fragment Antibody Library against Fusobacterium nucleatum". 2012. http://hdl.handle.net/10222/15184.
Texto completoFarhan Khan placed second in the International Association for Dental Research/Unilever Hatton Competition in the Senior Basic Science Research Category representing Canada, while presenting the research contained in this dissertation. This international competition took place during the 90th General Session & Exhibition of the International Association for Dental Research in Iguaçu Falls, Brazil in June 2012.
Capítulos de libros sobre el tema "CSF single chain library"
Nahary, Limor y Itai Benhar. "Design of a Human Synthetic Combinatorial Library of Single-Chain Antibodies". En Therapeutic Antibodies, 61–80. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-554-1_3.
Texto completoBecker, Richard C. y Frederick A. Spencer. "Novel Anticoagulants". En Fibrinolytic and Antithrombotic Therapy. Oxford University Press, 2006. http://dx.doi.org/10.1093/oso/9780195155648.003.0023.
Texto completoGeorgiou, George y Barrett R. Harvey. "Applications of Flow Cytometry in Protein Engineering". En Flow Cytometry for Biotechnology. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780195183146.003.0017.
Texto completoActas de conferencias sobre el tema "CSF single chain library"
Timofeev, S. A., A. A. Tsarev, V. S. Zhuravlev, A. V. Konarev y V. V. Dolgikh. "Construction of scFv-antibodies to the active center of the Sunn bug (Eurygaster integriceps Put) gluten-hydrolyzing protease GHP3". En 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.247.
Texto completoDahlbäck, B. y A. LundWall. "ISOLATION AND CHARACTERIZATION OF cDNA CLONES FOR HUMAN FACTOR V". En XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643886.
Texto completoLOFTUS, J. C., E. F. Plow, A. L. Frelinger III, M. A. Smith, S. D’ouza y M. H. Ginsberg. "LOCALIZATION AND CHEMICAL SYNTHESIS OF A DIVALENT CATION REGULATED EPITOPE IN PLATELET MEMBRANE GPIIb". En XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643959.
Texto completoKoide, T. "CHARACTERIZATION OF THE GENE FOR HUMAN HISTIDINE-RICH GLYCOPROTEIN". En XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643599.
Texto completoButler-Zimrin, A. E., J. S. Bennett, M. Poncz, E. Schwartz, S. Surrey, R. Eisman, R. A. Heidenreich y G. Vilaire. "ISOLATION AND CHARACTERIZATION OF cDNA CLONES FOR THE PLATELET MEMBRANE GLYCOPROTEINS IIb and IIIa". En XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643961.
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