Tesis sobre el tema "Cs41"
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Woods, Michael John. "CloudSpace: A Web Development Environment for CS1 Courses". Thesis, Virginia Tech, 2011. http://hdl.handle.net/10919/32880.
Texto completoMaster of Science
Parker, Joshua B. "Automation in CS1 with the Factoring Problem Generator". DigitalCommons@CalPoly, 2009. https://digitalcommons.calpoly.edu/theses/210.
Texto completoTsai, Yi-Ting. "Testicular macrophage regulation of Leydig cell development and function". Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25853.
Texto completoGarceau, Valerie. "Characterisation of the chicken mononuclear phagocyte system". Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/18006.
Texto completoFranzetti, Bruno. "Structure, fonction et expression de la protéine ribosomique chloroplastique CS1". Grenoble 1, 1992. http://www.theses.fr/1992GRE10099.
Texto completoMathsyaraja, Haritha. "CSF1 DRIVEN TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL ALTERATIONS IN MYELOID CELLS PROMOTE METASTATIC TUMOR PROGRESSION". The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1396965183.
Texto completoPrather, James. "Beyond Automated Assessment: Building Metacognitive Awareness in Novice Programmers in CS1". Diss., NSUWorks, 2018. https://nsuworks.nova.edu/gscis_etd/1030.
Texto completoDesfougères, Thomas. "Etude de l'effet de SFH2/CSR1 sur le métabolisme des acides gras chez "Saccharomyces cerevisiae"". Poitiers, 2007. http://www.theses.fr/2007POIT2333.
Texto completoDaly, Tebring. "Influence of Alice 3: Reducing the Hurdles to Success in a Cs1 Programming Course". Thesis, University of North Texas, 2013. https://digital.library.unt.edu/ark:/67531/metadc271795/.
Texto completoGarcia, Manuel. "An evaluation of potential technologies for a web based development environment for CS1 students /". Click here to view, 2009. http://digitalcommons.calpoly.edu/cscsp/2.
Texto completoProject advisor: David Janzen. Title from PDF title page; viewed on Jan. 14, 2010. Includes bibliographical references. Also available on microfiche.
García, Cervera Argelia Sarahí. "Diferenciación In Vitro de Osteoclastos de pollo mediante el cultivo con CSF1 y RANKL, y anotación funcional de genes". Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2017. http://hdl.handle.net/20.500.11799/68470.
Texto completoLos osteoclastos (OST) son células multinucleadas móviles de gran tamaño cuya función es reabsorber hueso. Su diferenciación es un proceso complejo que involucra la fusión entre tres o más precursores mononucleados y la posterior fijación al hueso. Las rutas moleculares que inciden en la diferenciación de los OST se han estudiado desde que se encontró que en ratón el co-cultivo de células estromales con médula ósea o con células del bazo daban como resultado OST. Debido a su importancia en la homeóstasis del hueso, su estudio resulta fundamental para la comprensión de los mecanismos de enfermedades óseas, como la osteoporosis o la osteopetrosis, A pesar del impacto que tienen estas enfermedades en la calidad de vida, particularmente la osteoporosis; los estudios hechos en OST hasta ahora, sólo se han llevado a cabo mediante la obtención de OST in vitro lavados directamente del hueso, de precursores de médula ósea cultivados sobre cortes de hueso, y más recientemente adicionando CSF1 y RANKL; únicamente a precursores de sangre periférica de humano, y a precursores en médula ósea de ratón; sin considerar las ventajas intrínsecas de efectuar la diferenciación in vitro en otros modelos de estudio ni las desventajas de las técnicas existentes. Aunque el genoma del pollo se encuentra completamente secuenciado, hasta el momento la anotación continúa siendo pobre, por lo que los estudios genéticos y moleculares en el pollo donde se empleen técnicas que permitan analizar la expresión de varios genes simultáneamente, tales como los microarreglos, requerirán de una anotación apropiada para su análisis. Efectuar una buena anotación es importante porque permite determinar si en la secuencia que se analiza existe un gen conocido, identificar la conservación de genes y vías de señalización entre organismos; para así poder extrapolar los resultados obtenidos a otras especies. El presente estudio tiene como objetivo diferenciar in vitro células precursoras monociticas de médula ósea en OST de pollo, así como realizar anotación funcional de varios genes expresados en OST; a fin de identificar genes específicos que pudieran en un futuro ser propuestos como blancos terapéuticos para enfermedades como la osteoporosis o la osteopetrosis. Para ello se optimizaron las condiciones requeridas para la producción de OST y MAC de pollo in vitro, posteriormente se realizó un microarreglo de ambos cultivos. Los datos devueltos por el microarreglo, se organizaron en función a los radios de expresión promedio entre ambos grupos. Se encontraron genes específicos de osteoclastos con homología en el pollo y otros organismos. Curiosamente, se encontraron marcos de lectura abierto enriquecidos en OST, sin embargo éstos presentan homología con los genomas de aves y no con los genomas del humano o del ratón.
DePasquale, Peter Joseph III. "Implications on the Learning of Programming Through the Implementation of Subsets in Program Development Environments". Diss., Virginia Tech, 2003. http://hdl.handle.net/10919/28367.
Texto completoPh. D.
Francisco, Rodrigo Elias. "Juiz online no ensino de CS1: requisitos, dificuldade de problemas e plágio em código-fonte". Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/6203.
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This dissertation approaches Online Judge in teaching Introductory Programming (CS1). Initially there was an exploratory research on BOCA system in teaching CS1, which brought experiences and data of student interactions with the system, which, supported by Systematic Literature Review (RSL), contributed to defining the requirements for the system meets the discipline of CS1 and guided continuing research. In the second phase, there was the aim to solve specific problems identified in the previous phase, and measuring the difficulty of CS1 problems and support for plagiarism identification in CS1 activities. The solution of these problems included RSL, practical experiences with writing and execution algorithms, comparison of the results with the expected results, and comparison of the proposed approaches to the identified in the literature. The strategy to measure the difficulty of problems CS1 proposed works with the height of a tree mounted to sets and sub-sets of nested code into a program and the amount of related subjects. The strategy to support the identification of plagiarism proposal works with the Edit Distance algorithm processing and normalization techniques in preprocessing, and it is a highly adapted proposal to the reality of the data used in this research (programs written in C with few lines of code by students CS1). Experience has shown the complexity of applying computing to education, which often works with subjective data, it was necessary to raise the difficulty of the problems in view of the students and the teacher’s view of the existence of plagiarism in peer programs, whose views are quite variables. It is suggested the creation of multidisciplinary teams to the evolution of the area (with professionals of computing, statistics, psychology and pedagogy) with a focus on validation and method used for research.
Esta dissertação aborda Juiz Online no ensino de Programação Introdutória (CS1). Inicialmente houve uma pesquisa exploratória sobre o sistema BOCA no ensino de CS1, que trouxe experiências e dados de interações de alunos com o sistema, que, apoiados pela Revisão Sistemática da Literatura (RSL), contribuíram para a definição dos requisitos necessários para que o sistema atenda à disciplina de CS1 e guiaram a continuidade da pesquisa. Em um segundo momento, o objetivo foi resolver problemas específicos levantados na fase anterior, sendo a medição da dificuldade de problemas de CS1 e o apoio à identificação de plágio em atividades de CS1. A solução desses problemas contou com RSL, experiências práticas com escrita e execução de algoritmos, comparação dos resultados obtidos com os resultados esperados, e comparação das abordagens propostas com as identificadas na literatura. A estratégia proposta para medir a dificuldade de problemas de CS1 trabalha com a altura de uma árvore montada com conjuntos e sub-conjuntos de códigos aninhados num programa e a quantidade de assuntos relacionados. A estratégia para apoiar a identificação de plágio proposta trabalha com o algoritmo Distância de Edição no processamento e técnicas de normalização no pré-processamento. Trata-se de uma proposta fortemente adaptada à realidade dos dados utilizados nesta pesquisa (programas escritos em C, com poucas linhas de código, por alunos de CS1). A experiência mostrou a complexidade em aplicar a computação à educação, que trabalha frequentemente com dados subjetivos. Foi necessário levantar a dificuldade dos problemas na visão dos alunos e a visão de professores sobre a existência de plágio em pares de programas, cujas opiniões são bastante variáveis. Sugere-se para a evolução da área, que sejam criadas equipes multidisciplinares (com profissionais de computação, estatística, psicologia e pedagogia) e haja um foco na validação e no método usado para as pesquisas.
Villain, Patricia. "Fonction transcriptionnelle du site 1 : élément cis du gène nucléaire d'épinard RPS1 codant pour la protéine ribosomique plastidiale CS1". Université Joseph Fourier (Grenoble), 1995. http://www.theses.fr/1995GRE10187.
Texto completoLi, Yuan. "Redox regulation of salicylic acid synthesis in plant immunity". Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/19578.
Texto completoPrice, Kellie W. "Using Visual Technologies in the Introductory Programming Courses for Computer Science Majors". NSUWorks, 2013. http://nsuworks.nova.edu/gscis_etd/276.
Texto completoJunqueira, Talita Vieta. "Expressão gênica de FOXP3, indoleamina 2,3 dioxigenase, IL10 e CSF1 em útero de vacas que receberam infusão intrauterina de antígenos maternos e paternos no período peri-ovulatório". Universidade Federal de Uberlândia, 2015. https://repositorio.ufu.br/handle/123456789/12416.
Texto completoA maioria das perdas gestacionais em bovinos acontece no início da gestação, particularmente entre os dias 7 e 16, período no qual o embrião é totalmente dependente do ambiente uterino para sobreviver e iniciar seu crescimento pré-implantação. Durante esse período, a mortalidade embrionária após transferência de embriões produzidos in vitro (TEPIV) ou in vivo (TE-OM) é em média quase duas vezes mais elevada do que aquela derivada de embriões originados de monta natural ou inseminação artificial (IA). A sensibilização da receptora contra as moléculas MHC paternas e maternas do embrião alogênico pode ser uma das causas das altas taxas de perdas gestacionais observadas após TE. Estudos realizados em humanos e em várias espécies têm demonstrado que a sensibilização com antígenos do concepto pode ser uma maneira útil de afetar o desempenho reprodutivo facilitando o reconhecimento e a aceitação materna do embrião alogênico através da indução de citocinas e células imunorregulatórias no microambiente uterino. Nesse sentido, o presente estudo teve como foco principal determinar se a administração simultânea ou isolada de antígenos paterno e materno no útero de fêmeas bovinas receptoras de embrião PIV, no dia do estro, aumenta a expressão de genes que podem facilitar o reconhecimento e desenvolvimento do embrião alogênico durante o início da gestação. Para isto, foram utilizadas 45 vacas cruzadas divididas em 4 tratamentos: T0: controle; T1: Sêmen; T2: PBMCs e T3: PBMCs+Sêmen. As fêmeas bovinas foram sincronizadas ao estro e receberam os antígenos no corpo uterino no dia do cio (D0). Biópsias uterinas foram coletadas in vivo no D0, para controle, e 7 (D7) e 14 (D14) dias após o cio e a administração dos antígenos, para avaliar o efeito do tratamento no ambiente uterino da receptora no momento em que ocorreria o procedimento de anovulação em TE-PIV e durante o período no qual o embrião bovino já teria iniciado seu crescimento préimplantação, respectivamente. A expressão gênica foi avaliada por PCR em tempo real e transcritos de FOXP3, IDO, IL-10 e CSF-1 foram detectados em todas as amostras de RNA extraídas das biópsias uterinas. A análise semiquantitativa da expressão gênica relativa entre os grupos controle e tratado mostrou que nenhum dos tratamentos promoveu aumento significativo na expressão desses genes. Além disso, no D14 todos os tratamentos promoveram uma queda na quantidade de transcritos de CSF-1 e, ainda, o tratamento com ambos os antígenos também promoveu uma queda na abundância de transcritos de IL-10. Em conclusão, a administração isolada ou simultânea de ambos os antígenos no útero de vacas receptoras de embrião PIV parece não propiciar aumento da tolerância materna aos aloantígenos do embrião nem condições favoráveis a seu crescimento e desenvolvimento préimplantação, pelo menos no que se refere ao efeito mediado por FOXP3, IDO, IL-10 e CSF-1 no D7 e D14 do ciclo estral.
Mestre em Biologia Celular e Estrutural Aplicadas
Lisowski, Zofia Maria. "Targeting the macrophage in equine post-operative ileus". Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/33191.
Texto completoNielsen, Bryce. "Developing response surfaces based on tool geometry for a convex scrolled shoulder step spiral (CS4) friction stir processing tool used to weld Al 7075 /". Diss., CLICK HERE for online access, 2009. http://contentdm.lib.byu.edu/ETD/image/etd2813.pdf.
Texto completoNielsen, Bryce K. "Developing Response Surfaces Based on Tool Geometry for a Convex Scrolled Shoulder Step Spiral (CS4) Friction Stir Processing Tool Used to Weld AL 7075". BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/1782.
Texto completoGriffin, Jean. "Learning to Program From Interactive Example Code (With and Without Intentional Bugs)". Diss., Temple University Libraries, 2018. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/488614.
Texto completoPh.D.
Computing education for learning to program has made great strides in the current century. Exciting educational technologies are now available and active learning pedagogies are increasingly used. Interest is strong, but the longstanding problem remains: learning to program as an analytical endeavor is quite frustrating for many. The purpose of this study is to discover ways to mitigate this frustration. It researches ways to help students comprehend code by guiding them to take it apart (through reading, tracing, completing, and debugging) as they learn to write code on their own. This study contributes to the understanding of learning from errors. It also builds upon and further develops the emergent pedagogy of de-constructionism. The de-constructionist approach involves taking things apart, practice, and learning from errors. This study applies a de-constructionist approach in an experiment with ~80 undergraduates learning Python in an introductory programming class. During weekly lab periods, students engaged with web-based interactive practice problems that emphasize reading, tracing, completing, and in some cases, debugging code. Students also wrote code for lab and homework assignments. Approximately half of the students were given some that involved learning from bugs that were intentionally placed in the provided code, while the others were not. Learning gains were assessed using pre/post tests and exams. Surveys were used to measure attitudes. Learning gains and attitudes were compared according to condition (Bugs, NoBugs), prior experience, gender, minority status, and class size. This study demonstrates that bugs can be intentionally incorporated into practice problems that students like to solve, without detrimental effects on learning or attitudes about computing. It also contributes to the literature on code comprehension.
Temple University--Theses
Ryu, Mike Dongyub. "Improving Introductory Computer Science Education with DRaCO". DigitalCommons@CalPoly, 2018. https://digitalcommons.calpoly.edu/theses/1943.
Texto completoMoraes, Tatiana de Souza. "Transformação genética de tomateiro (Solanum lycopersicum cv. \'Micro-Tom\') e de laranja doce (Citrus sinensis L. Osbeck) com o gene Csd1 (superóxido dismutase do cobre e do zinco), isolado de Poncirus trifoliata". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-14122015-103829/.
Texto completoAlthough the citrus industry is an important economic activity in Brazil, in recent years there has been a significant reduction in the national citrus production. The low profitability of the citrus sector has faced due to the high production cost is mainly attributed to phytosanitary problems, particularly diseases that directly affect productivity of orchards. Currently, huanglongbing (HLB) is the most serious disease that affects the global citrus industry and the damage is severe in all citrus varieties. Genetic transformation of plants is an alternative to obtain transgenic plants with genes that stimulate the plant defense system, making it resistant to diseases. Despite the effectiveness of protocols for genetic transformation of citrus, a characteristic disadvantage of perennial plants is the slow reproductive cycle, hindering validation of new genes of interest. Therefore, an important strategy is the use of model plants, such as the tomato, which has a short cycle and good genetic transformation efficiency. The objective of this study was to obtain transgenic plants of Solanum lycopersicum cv. \"Micro-Tom \'and Citrus sinensis, containing the gene construct with Csd1 gene (copper/zinc superoxide dismutase), isolated of Poncirus trifoliata. The protein encoded by the gene Csd1, also known as SOD1 (superoxide dismutase 1), is the most powerful antioxidant in nature and is important constituent of cellular defense against oxidative stress caused by bacterial infection. \'Micro-tom\' tomato was used as a model for pathogenic gene validation. However, due to its low efficiency of genetic transformation, the inoculation experiments with the pathogen were not realized. Posteriorly, the characterization of gene function Csd1 in relation to the HLB disease will be realize with citrus transgenic plants. The objective of this study was to obtain transgenic plants of Solanum lycopersicum cv. \'Micro-Tom\' and of Citrus sinensis, containing the gene construction with Csd1 gene (copper/zinc superoxide dismutase), isolated of Poncirus trifoliata, for validation and for future study of this gene for resistance to HLB. Proteins encoded by the Csd1 gene, also known as SOD1 (superoxide dismutase 1), are the most powerful antioxidants in nature and are important constituents of cellular defense against oxidative stress caused by bacterial infection. The identification of transgenic plants of tomato and sweet orange was performed by the PCR analysis using primers for the detection of Csd1 gene. The PCR+ plants were acclimatized and transferred to a greenhouse. The genetic transformation efficiency of tomato \'Micro-Tom\' and sweet orange cultivars, \'Hamlin\' and \'Pineapple\', were 0.34%, 4.74% and 3.65%, respectively. The molecular characterization with the Southern blot and RT-qPCR analyses was performed only in citrus plants. The transgene integration was confirmed in 32 plants. The number of insertion events ranged from 1-5 and the presence of Csd1 endogenous gene is found in three distinct locations in the plants genome. The mRNA level of the transgene was verified in 21 plants that had only a single transgene insertion into the plant genome. The results show that there was transcription of Csd1 gene in transgenic plants as well as in non-transgenic plants. The relation between the transgene transcript level with the resistance of plants to pathogens is set after inoculation with Candidadus Liberibacter.
Fernandez, Reinaldo. "A Cognitive Apprenticeship Approach for Teaching Abstract and Complex Skills in an Online Learning Environment". NSUWorks, 2014. http://nsuworks.nova.edu/gscis_etd/2.
Texto completoGilley, William. "Animations and Interactive Material for Improving the Effectiveness of Learning the Fundamentals of Computer Science". Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/32899.
Texto completoDue to the rapid proliferation of the World Wide Web (WWW) in recent years, many educators are now seeking to improve the effectiveness of their instruction by providing interactive, web-based course material to their students. The purpose of this thesis is to document a set of eight online learning modules created to improve the effectiveness of learning the fundamentals of Computer Science. The modules are as follows:
- Algorithms - Definition and specification of algorithms, with a comparison and analysis of several sorting algorithms as examples.
- Artificial Intelligence - Overview of current applications in this discipline.
- Data Structures - Explanation of basic data structures, including an introduction to computer memory and pointers, and a comparison of logical and physical representations of commonly used data structures.
- Machine Architecture - Explanation of data storage, gates and circuits, and the central processing unit.
- Number Systems - Discussion of number representation and arithmetic in number systems other than the decimal number system, with a focus on binary numbers and binary arithmetic.
- Operating Systems - Explanation of the purpose of operating systems and the major components that make up an operating system.
- Programming Languages - Explanation of the fundamental concepts in procedural programming languages.
- Software Engineering - Introduction to software life cycle models and an overview of the procedural and object-oriented paradigms.
Each module consists of a set of lessons and review questions written in HyperText Markup Language (HTML). Embedded in these pages are various interactive components implemented as Flash animations or Java applets. The modules currently reside on the Computer Science courseware server of Virginia Polytechnic Institute and State University (Virginia Tech) and can be viewed at the following WWW site: http://courses.cs.vt.edu/csonline/.
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Master of Science
Tung-ShengLin y 林東昇. "Regulation of a novel adhesin Csp1 in Clostridium difficile". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/24xnw4.
Texto completo國立成功大學
微生物及免疫學研究所
103
Clostridium difficile is a Gram positive, spore forming obligate anaerobic bacteria, causive agent of the antibiotic associated diarrhea. C. difficile infection (CDI) is caused by host microflora disruption through broad-spectrum antibiotics. The emergence of hypervirulent C. difficile strains resulting in high morbidity and mortality has occurred in many countries. The C. difficile secrets toxins TcdA, TcdB and binary toxin CDT. TcdA and TcdB are responsible for gastrointestinal inflammation, epithelial cell tight junction lose and apoptosis. Before infection, the pathogens need to attach to the host cell first. In C. difficile, surface protein are responsible for interaction with host cell and extracellular matrix of vertebrates. Csp1, a potential cell wall protein anchored on cell wall by sortase, and a putative collagen binding adhesin. Zmp1, a metalloprotease which can cleave Csp1 in vitro. c-di-GMP, a second messenger molecules modulate C. difficile motility, biofilm and toxin. In our study, we focus on Csp1 regulation between Zmp1 and c-di-GMP. Our results showed that in the absence of metalloprotease, Csp1 localization is increased on the cell wall, c-di-GMP enhance the quantity of Csp1 , and the PPKTG motif is needed for recognization by sortase. In summary, our results demonstrated the localization and regulation of a novel adhesin Csp1 by Zmp1 and c-di-GMP.
Zih-CianSu y 蘇資茜. "Molecular characterization of a putative adhesin Csp1 in Clostridium difficile". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/25453058743918092331.
Texto completo國立成功大學
微生物及免疫學研究所
102
Clostridium difficile is a Gram-positive, endospore forming human pathogen and the most common cause of antibiotic-associated diarrhea within hospital settings worldwide. Disruption of the host’s indigenous microflora by broad-spectrum antibiotics is one of the major triggers for C. difficile infections (CDI). The emergence of hypervirulent C. difficile strains with high mortality rates have resulted in major epidemics in many parts of the world. C. difficile can express two exotoxins TcdA and TcdB, and in some strains a binary toxin CDT. Both TcdA and TcdB are potent toxins and are responsible for the extensive gastrointestinal inflammation and epithelial tissue damages found in an infected host. In addition, C. difficile is known to express a wide-variety of surface proteins such as S-layer proteins, fibronectin and cell wall proteins (Cwps). Surface proteins of Gram-positive bacteria are known to play important roles during infections. Sortase, a membrane anchored transpeptidase found ubiquitously in Gram-positive bacteria, promotes the covalent anchoring of surface proteins to the cell wall envelope. To determine the role of sortase-dependent surface proteins in C. difficile pathogenesis, I identified at least seven putative sortase substrates in C. difficile strain 630 by bioinformatics analysis. In this study, I focused on one of the putative cell surface proteins, Csp1. Csp1, encoded by CD2831, is a predicted protein of 972 amino acids and is annotated as a collagen-binding protein. The protein has a PPKTG motif suggesting that Csp1 could be a putative substrate of C. difficile sortase B. The aim of this study is to characterize molecular properties of Csp1. I constructed csp1 insertion mutant, KYC01, and defined the localization of Csp1 by immunoblotting using polyclonal anti-Csp1. Due to the low expression level of Csp1, I also overexpressed Csp1 in both CD630 and KYC01. Cellular fractionation and immunoblotting analysis demonstrated that Csp1 can be found anchored to the cell wall. Interestingly, the majority of Csp1 appear to be secreted into the medium. In-vitro binding experiments demonstrated the ability of Csp1 recombinant protein to adhere to Collagen I. In summary, the results generated from this project revealed a novel adhesin in C. difficile.
Hsu, Kuo-Sheng y 徐國盛. "Coexpression,copurification of Sacchromyces cerevisiae monopolin complex and analysis of Csm1/Lrs4 complex stoichiometry". Thesis, 2004. http://ndltd.ncl.edu.tw/handle/57847740861935079287.
Texto completo國立臺灣大學
生化科學研究所
92
In budding yeast, Saccharomyces cerevisiae, Mam1, Csm1 and Lrs4 form monopolin complex around sister centromeres during meiosis I for sister chromatids cosegregation. Sister kinetochores can be clamped togethor by monopolin complex to attach the same oriented poles during metaphase and the sister chromatids will be pulled to the same cell after the first cell division of meiosis. For studying the protein complex, we used the high-throughput screening of soluble recombinant proteins method to obtain the clones which could overexpress the target fusion protein. By transforming two hetero-locus plasmids to E. coli., BL21-CodonPlus(DE3), Csm1/Lrs4 or Mam1/Csm1/Lrs4 complex was coexpressed in the double antibiotic selected strains. Using Ni-NTA or amylase affinity column, we have purified His6-Csm1, His6-Csm1/Lrs4 and MBP-Mam1/His6-Csm1/Lrs4 protein complex indepedently. Futhermore, we also determined the stoichiometry of Csm1/Lrs4 complex by combining the gel filtration and the sucrose gradient velocity sedimentation assay. In conclusion, we confirmed Mam1, Csm1 and Lrs4 can form monopolin complex in vitro and Csm1 can form homotrimer alone and heterohexamer with Lrs4 via the interaction of their coiled coil domains.
"Monosporic cultures cytology and fertility and Agaricus blazei CS1 mating results". Tese, BIBLIOTECA CENTRAL DA UFLA, 2006. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=169.
Texto completoSchroeder, Andrew J. "CSE1, an essential yeast gene required for cell cycle progression, encodes a nuclear transport factor". 1998. https://scholarworks.umass.edu/dissertations/AAI9823773.
Texto completoEstey, Anthony. "An exploration of learning tool log data in CS1: how to better understand student behaviour and learning". Thesis, 2016. http://hdl.handle.net/1828/7757.
Texto completoGraduate
0984
0525
0710
aestey@uvic.ca
Haney, Mickey D. "A structured approach to teach the content of CS1 to non-major students with an emphasis on analytic problem solving". 1987. http://hdl.handle.net/2097/23664.
Texto completoBecker, Daniel. "Maturation and nucleo-cytoplasmic shuttling of snRNAs in Saccharomyces cerevisiae". Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E56A-D.
Texto completoRusso, Kennedy Anna. "Towards a Data-Driven Analysis of Programming Tutorials' Telemetry to Improve the Educational Experience in Introductory Programming Courses". Thesis, 2015. http://hdl.handle.net/1828/6500.
Texto completoGraduate
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Schönitzer, Veronika [Verfasser]. "Dictyostelium discoideum als Expressionssystem für die transmembrane Myosin-Chitinsynthase Ar-CS1 aus Atrina rigida (Mollusca, Bivalvia) - einem Modellorganismus der Biomineralisation - und Charakterisierung der Myosindomäne / vorgelegt von Veronika Schönitzer". 2009. http://d-nb.info/1002001897/34.
Texto completo(9189365), Anthony A. Lowe. "The Theory of Applied Mind of Programming". Thesis, 2020.
Buscar texto completoThe Theory of Applied Mind of Programming (TAMP) provides a new model for describing how programmers think and learn. Historically, many students have struggled when learning to program. Programming as a discipline lives in logic and reason, but theory and science tell us that people do not always think rationally. TAMP builds upon the groundbreaking work of dual process theory and classical educational theorists (Piaget, Vygotsky, and Bruner) to rethink our assumptions about cognition and learning. Theory guides educators and researchers to improve their practice, not just their work but also their thinking. TAMP provides new theoretical constructs for describing the mental activities of programming, the challenges in learning to program, as well as a guidebook for creating and recognizing the value of theory.
This dissertation is highly nontraditional. It does not include a typical empirical study using a familiar research methodology to guide data collection and analysis. Instead, it leverages existing data, as accumulated over a half-century of computing education research and a century of research into cognition and learning. Since an applicable methodology of theory-building did not exist, this work also defines a new methodology for theory building. The methodology of this dissertation borrows notation from philosophy and methods from grounded theory to define a transparent and rigorous approach to creating applied theories. By revisiting past studies through the lens of new theoretical propositions, theorists can conceive, refine, and internally validate new constructs and propositions to revolutionize how we view technical education.
The takeaway from this dissertation is a set of new theoretical constructs and promising research and pedagogical approaches. TAMP proposes an applied model of Jerome Bruner's mental representations that describe the knowledge and cognitive processes of an experienced programmer. TAMP highlights implicit learning and the role of intuition in decision making across many aspects of programming. This work includes numerous examples of how to apply TAMP and its supporting theories in re-imagining teaching and research to offer alternative explanations for previously puzzling findings on student learning. TAMP may challenge conventional beliefs about applied reasoning and the extent of traditional pedagogy, but it also offers insights on how to promote creative problem-solving in students.