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1

Lyrene, Paul M. "Citrus: Genetics, Breeding and Biotechnology." HortScience 43, no. 6 (October 2008): 1932a—1932. http://dx.doi.org/10.21273/hortsci.43.6.1932a.

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2

Gmitter, Frederick G. "Contemporary Approaches to Improving Citrus Cultivars." HortTechnology 4, no. 3 (July 1994): 206–10. http://dx.doi.org/10.21273/horttech.4.3.206.

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Traditional genetic manipulation methods have proven ineffective or irrelevant for many citrus breeding objectives. Alternative approaches to Citrus genetic improvement are now available as a result of technological developments in genetics and tissue culture. For example, mapping DNA marker polymorphisms should lead to identifying markers closely linked to important loci, thereby facilitating early selection and minimizing costs associated with plant size and juvenility. Genetic transformation methods allow trait-specific modification of commercial cultivars. By selecting beneficial variants
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3

Talon, Manuel, and Fred G. Gmitter. "Citrus Genomics." International Journal of Plant Genomics 2008 (May 19, 2008): 1–17. http://dx.doi.org/10.1155/2008/528361.

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Citrus is one of the most widespread fruit crops globally, with great economic and health value. It is among the most difficult plants to improve through traditional breeding approaches. Currently, there is risk of devastation by diseases threatening to limit production and future availability to the human population. As technologies rapidly advance in genomic science, they are quickly adapted to address the biological challenges of the citrus plant system and the world's industries. The historical developments of linkage mapping, markers and breeding, EST projects, physical mapping, an intern
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4

Gmitter, Frederick G. "CONTEMPORARY APPROACHES TO CITRUS CULTIVAR IMPROVEMENT." HortScience 27, no. 6 (June 1992): 699b—699. http://dx.doi.org/10.21273/hortsci.27.6.699b.

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Traditional methods of genetic manipulation have proven ineffective or irrelevant for many citrus breeding objectives. Alternative approaches to genetic improvement of citrus are now available as a result of technological developments in genetics and tissue culture. Mapping DNA markers on the Citrus genome should lead to identification of markers closely linked to important loci, thereby facilitating early selection and minimizing costs associated with plant size and juvenility. Genetic transformation methods provide opportunities for trait-specific modification of commercial cultivars. The se
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5

Omura, Mitsuo, and Takehiko Shimada. "Citrus breeding, genetics and genomics in Japan." Breeding Science 66, no. 1 (2016): 3–17. http://dx.doi.org/10.1270/jsbbs.66.3.

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6

Donmez, Dicle, Ozhan Simsek, Tolga Izgu, Yildiz Aka Kacar, and Yesim Yalcin Mendi. "Genetic Transformation inCitrus." Scientific World Journal 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/491207.

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Citrus is one of the world’s important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs.Citrustransformation has now been achieved in a number of laboratories by various methods.Agrobacterium tumefaciensis used mainly in citrus transformation studies. Particle bombardment, electroporation,A. rhizogenes, and a new method called RNA int
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7

Elleuch, Amine, Fattouma Djilani Khouaja, Imen Hamdi, Nabiha Bsais, Jean-Pierre Perreault, Mohamed Marrakchi, and Hatem Fakhfakh. "Sequence analysis of three citrus viroids infecting a single Tunisian citrus tree (Citrus, reticulata, Clementine)." Genetics and Molecular Biology 29, no. 4 (2006): 705–10. http://dx.doi.org/10.1590/s1415-47572006000400020.

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8

Gmitter, Fred G., Chunxian Chen, Marcos A. Machado, Alessandra Alves de Souza, Patrick Ollitrault, Yann Froehlicher, and Tokurou Shimizu. "Citrus genomics." Tree Genetics & Genomes 8, no. 3 (April 26, 2012): 611–26. http://dx.doi.org/10.1007/s11295-012-0499-2.

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9

Brune, Andreas, Mathias Müller, Lincoln Taiz, Pedro Gonzalez, and Ed Etxeberria. "Vacuolar Acidification in Citrus Fruit: Comparison between Acid Lime (Citrus aurantifolia) and Sweet Lime (Citrus limmetioides) Juice Cells." Journal of the American Society for Horticultural Science 127, no. 2 (March 2002): 171–77. http://dx.doi.org/10.21273/jashs.127.2.171.

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Vacuolar acidification was investigated in `Palestine' sweet (Citrus limmetioides Tanaka) and `Persian' acid lime [(Citrus aurantifolia (Christm.) Swingle] (vacuolar pHs of 5.0 and 2.1, respectively) using tonoplast vesicles isolated from juice cells. The ATPase activity of tonoplast-enriched vesicles from sweet limes was strongly inhibited by bafilomycin A1 and NO3-, but was unaffected by vanadate. In contrast, the ATPase activity in acid lime membranes was only slightly inhibited by bafilomycin A1 and NO3- and was strongly inhibited by high concentrations of vanadate. The vacuolar origin of
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10

Yu, Yuan, Chunxian Chen, Ming Huang, Qibin Yu, Dongliang Du, Matthew R. Mattia, and Frederick G. Gmitter. "Genetic Diversity and Population Structure Analysis of Citrus Germplasm with Single Nucleotide Polymorphism Markers." Journal of the American Society for Horticultural Science 143, no. 6 (November 2018): 399–408. http://dx.doi.org/10.21273/jashs04394-18.

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Citrus (Citrus sp.) germplasm collections are a valuable resource for citrus genetic breeding studies, and further utilization of the resource requires knowledge of their genotypic and phylogenetic relationships. Diverse citrus accessions, including citron (Citrus medica), mandarin (Citrus reticulata), pummelo (Citrus maxima), papeda (Papeda sp.), trifoliate orange (Poncirus trifoliata), kumquat (Fortunella sp.), and related species, have been housed at the Florida Citrus Arboretum, Winter Haven, FL, but the accessions in the collection have not been genotyped. In this study, a collection of 8
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11

Bernet, G. P., C. Margaix, J. Jacas, E. A. Carbonell, and M. J. Asins. "Genetic analysis of citrus leafminer susceptibility." Theoretical and Applied Genetics 110, no. 8 (April 16, 2005): 1393–400. http://dx.doi.org/10.1007/s00122-005-1943-6.

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12

Liu, Qiyan, Song Zhang, Shiqiang Mei, Yan Zhou, Jianhua Wang, Guan-Zhu Han, Lei Chen, Changyong Zhou, and Mengji Cao. "Viromics unveils extraordinary genetic diversity of the family Closteroviridae in wild citrus." PLOS Pathogens 17, no. 7 (July 12, 2021): e1009751. http://dx.doi.org/10.1371/journal.ppat.1009751.

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Our knowledge of citrus viruses is largely skewed toward virus pathology in cultivated orchards. Little is known about the virus diversity in wild citrus species. Here, we used a metatranscriptomics approach to characterize the virus diversity in a wild citrus habitat within the proposed center of the origin of citrus plants. We discovered a total of 44 virus isolates that could be classified into species Citrus tristeza virus and putative species citrus associated ampelovirus 1, citrus associated ampelovirus 2, and citrus virus B within the family Closteroviridae, providing important informat
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13

Burns, Jacqueline K., Luis V. Pozo, Covadonga R. Arias, Brandon Hockema, Vidhya Rangaswamy, and Carol L. Bender. "Coronatine and Abscission in Citrus." Journal of the American Society for Horticultural Science 128, no. 3 (May 2003): 309–15. http://dx.doi.org/10.21273/jashs.128.3.0309.

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Coronatine is a polyketide phytotoxin produced by several plant pathogenic Pseudomonas spp. The effect of coronatine on abscission in Citrus sinensis L. Osbeck `Hamlin' and `Valencia' orange fruit, leaves, fruitlets, and flowers was determined. Coronatine at 200 mg·L-1 significantly reduced fruit detachment force of mature fruit, and did not cause fruitlet or flower loss in `Valencia'. Cumulative leaf loss was 18% with coronatine treatment. Coronafacic acid or coronamic acid, precursors to coronatine in Pseudomonas syringae, did not cause mature fruit abscission. Ethylene production in mature
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14

Lea-Cox, John D., and James P. Syvertsen. "Nitrogen Uptake by Citrus Leaves." Journal of the American Society for Horticultural Science 120, no. 3 (May 1995): 505–9. http://dx.doi.org/10.21273/jashs.120.3.505.

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We studied whether foliar-applied N uptake from a single application of low-biuret N-urea or K NO to citrus leaves was affected by N source, leaf age, or whole-shoot N content. In a glasshouse experiment using potted 18-month-old Citrus paradisi (L.) `Redblush' grapefruit trees grown in full sun, 2- and 6-month-old leaves on single shoots were dipped into a 11.2 g N/liter (1.776% atom excess N-urea) solution with 0.1% (v/v) Triton X-77. Two entire trees were harvested 1.5,6,24, and 48 hours after N application. Uptake of N per unit leaf area was 1.6- to 6-fold greater for 2-month-old leaves th
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15

Marin, M. L., and N. Duran-Vila. "Conservation of Citrus Germplasm in Vitro." Journal of the American Society for Horticultural Science 116, no. 4 (July 1991): 740–46. http://dx.doi.org/10.21273/jashs.116.4.740.

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A study was conducted to evaluate the potential of in vitro techniques for genetic conservation of citrus. A tissue culture system was developed using explants of juvenile `Pineapple' sweet orange. It consisted of: a) establishment of primary cultures from nodal stem segments followed by the recovery of plants in vitro; and b) successive cycles of secondary cultures consisting of the culture of nodal stem segments from in vitro-grown plants, rooting of shoots obtained from nodal stem segments, and recovery of whole plantlets. Two parameters, K and K', based on the multiplication factors of the
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16

Freitas-Astúa, Juliana, Marinês Bastianel, Eliane C. Locali-Fabris, Valdenice M. Novelli, Ana Carla Silva-Pinhati, Ana Carolina Basílio-Palmieri, Maria Luisa P. N. Targon, and Marcos A. Machado. "Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction." Genetics and Molecular Biology 30, no. 3 suppl (2007): 980–90. http://dx.doi.org/10.1590/s1415-47572007000500026.

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17

Jude W. Grosser, Frederick G. Gmitter Jr., Manjul Dutt, Milica Calovic, Paul Ling, and Bill Castle. "HIGHLIGHTS OF THE UNIVERSITY OF FLORIDA, CITRUS RESEARCH AND EDUCATION CENTER'S COMPREHENSIVE CITRUS BREEDING AND GENETICS PROGRAM." Acta Horticulturae, no. 1065 (January 2015): 405–13. http://dx.doi.org/10.17660/actahortic.2015.1065.50.

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18

Zou, Xiuping, Meixia Du, Yunuo Liu, Liu Wu, Lanzhen Xu, Qin Long, Aihong Peng, Yongrui He, Maxuel Andrade, and Shanchun Chen. "CsLOB1 regulates susceptibility to citrus canker through promoting cell proliferation in citrus." Plant Journal 106, no. 4 (March 23, 2021): 1039–57. http://dx.doi.org/10.1111/tpj.15217.

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19

Taylor, K. C., and H. L. Geitzenauer. "5-kD Zinc-binding Protein Accumulation in Macrophylla-decline-affected Citrus." Journal of the American Society for Horticultural Science 123, no. 3 (May 1998): 357–60. http://dx.doi.org/10.21273/jashs.123.3.357.

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Macrophylla-decline (MD)-affected citrus display apparent nutrient deficiencies in a sectorial pattern within the citrus tree canopy. The status of several elements (Ca, Cu, Fe, Mg, Mn, and Zn) was assessed in MD and healthy citrus selected from the same citrus orchards. Leaf and phloem tissues were sampled from mature, reproductive trees. Levels of Ca, Cu, Fe, Mg, and Mn were unaffected by the disorder in leaf or phloem tissues. Zinc was diminished in the leaves of MD citrus, and elevated in the whole phloem tissue (2.57-fold on a dry mass basis). Calcium and Cu were sufficient, while Mg, Fe,
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20

Tisserat, Brent, Danny Jones, and Paul D. Galletta. "Natural Branching in Citrus Juice Vesicles." Journal of the American Society for Horticultural Science 113, no. 6 (November 1988): 957–60. http://dx.doi.org/10.21273/jashs.113.6.957.

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Abstract Juice vesicles in several Citrus species and cultivars differentiate additional vesicle apex primordia. Four distinct types of juice vesicles were described and quantified in citrus fruits: solitary (single stalk/single vesicle body/single apex primordium), dual-tipped (single stalk/single vesicle body/dual apex primordia), multiple-tipped (single stalk/single vesicle body/multiple apex primordia) and branched (single-stalk/multiple vesicle bodies/multiple apex primordia). The type and frequency of branching varies considerably among species and within cultivars. Branching was found i
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21

Achor, D. S., H. Browning, and L. G. Albrigo. "Anatomical and Histochemical Effects of Feeding by Citrus Leafminer Larvae (Phyllocnistis citrella Stainton) in Citrus Leaves." Journal of the American Society for Horticultural Science 122, no. 6 (November 1997): 829–36. http://dx.doi.org/10.21273/jashs.122.6.829.

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Young expanding leaves of `Ambersweet' [Citrus reticulata Blanco × C. paradisi Macf. × C. reticulata) × C. sinensis (L) Osb.] with feeding injury by third larval stage of citrus leafminer (Phyllocnistis citrella) were examined by light and electron microscopy for extent of injury and tissue recovery over time. Results confirmed that injury is confined to the epidermal layer, leaving a thin covering over the mine tunnel that consisted of the cuticle and outer cell wall. Wound recovery consisted of two possible responses: the production of callus tissue or the formation of wound periderm. The pr
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22

Ahmad, Riaz, Darush Struss, and Stephen M. Southwick. "Development and Characterization of Microsatellite Markers in Citrus." Journal of the American Society for Horticultural Science 128, no. 4 (July 2003): 584–90. http://dx.doi.org/10.21273/jashs.128.4.0584.

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We evaluated the potential of microsatellite markers for use in Citrus genome analysis. Microsatellite loci were identified by screening enriched and nonenriched libraries developed from `Washington Navel' Citrus. Microsatellite-containing clones were sequenced and 26 specific PCR primers were selected for cross-species amplification and identification of cultivars/clones in Citrus. After an enrichment procedure, on average 69.9% of clones contained dinucleotide repeats (CA)n and (CT)n, in contrast to <25% of the clones that were identified as positive in hybridization screening of a nonenr
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23

Bordignon, Rita, Herculano Penna Medina-Filho, Walter José Siqueira, and Joaquim Teófilo Sobrinho. "The genetics of tolerance to tristeza disease in citrus rootstocks." Genetics and Molecular Biology 27, no. 2 (2004): 199–206. http://dx.doi.org/10.1590/s1415-47572004000200013.

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24

Khalil, Ashraf T., Galal T. Maatooq, and Khalid A. El Sayed. "Limonoids from Citrus reticulata." Zeitschrift für Naturforschung C 58, no. 3-4 (April 1, 2003): 165–70. http://dx.doi.org/10.1515/znc-2003-3-403.

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The seeds of Citrus reticulata afforded the new limonoid derivative, isolimonexic acid methyl ether, in addition to the the previously isolated limonin, deacetylnomilin, obacunone and ichangin. The structure elucidation was achieved primarily through 1D and 2-D-NMR analyses. The marginal antimalarial activity of isolimonexic acid methyl ether is reported.
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25

Lyu, Shiheng, Ying Yu, Shirong Xu, Weiwei Cai, Guixin Chen, Jianjun Chen, Dongming Pan, and Wenqin She. "Identification of Appropriate Reference Genes for Normalizing miRNA Expression in Citrus Infected by Xanthomonas citri subsp. citri." Genes 11, no. 1 (December 23, 2019): 17. http://dx.doi.org/10.3390/genes11010017.

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MicroRNAs (miRNAs) are short noncoding RNA molecules that regulate gene expression at the posttranscriptional level. Reverse transcription-quantitative PCR (RT-qPCR) is one of the most common methods used for quantification of miRNA expression, and the levels of expression are normalized by comparing with reference genes. Thus, the selection of reference genes is critically important for accurate quantification. The present study was intended to identify appropriate miRNA reference genes for normalizing the level of miRNA expression in Citrus sinensis L. Osbeck and Citrus reticulata Blanco inf
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26

Catalano, Chiara, Angelo Ciacciulli, Fabrizio Salonia, Maria Patrizia Russo, Paola Caruso, Marco Caruso, Giuseppe Russo, Gaetano Distefano, and Concetta Licciardello. "Target-Genes Reveal Species and Genotypic Specificity of Anthocyanin Pigmentation in Citrus and Related Genera." Genes 11, no. 7 (July 16, 2020): 807. http://dx.doi.org/10.3390/genes11070807.

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Background: Anthocyanin pigmentation characterizes a number of tissues of Citrus and its relatives. The gain and loss of pigmentation is intriguing and is inherited variously among species. Methods: Citrus germplasm was used to investigate the anthocyanin pigmentation of tissues never before considered, including stamen, style and stigma, and of young leaves, petals, rind and flesh of 28 genotypes belonging to 14 species. Citrus genotypes encompassed citron, lemon, sweet orange, lime, and Citrus relatives included Microcitrus, Murraya, and Severinia. A relative qRT-PCR analysis was carried out
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27

Tipu, Mohammad Monirul Hasan, MD Mashiur Rahman, Md Mynul Islam, Ferdous-E. Elahi, Raunak Jahan, and Md Rashidul Islam. "Citrus greening disease (HLB) on Citrus reticulata (Mandarin) caused by Candidatus Liberibacter asiaticus in Bangladesh." Physiological and Molecular Plant Pathology 112 (December 2020): 101558. http://dx.doi.org/10.1016/j.pmpp.2020.101558.

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28

Lewinsohn, Efraim, Lothar Britsch, Yehuda Mazur, and Jonathan Gressel. "Flavanone Glycoside Biosynthesis in Citrus." Plant Physiology 91, no. 4 (December 1, 1989): 1323–28. http://dx.doi.org/10.1104/pp.91.4.1323.

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29

Koch, Karen E., and Wayne T. Avigne. "Postphloem, Nonvascular Transfer in Citrus." Plant Physiology 93, no. 4 (August 1, 1990): 1405–16. http://dx.doi.org/10.1104/pp.93.4.1405.

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30

Jourdan, Pablo S., Elmar W. Weiler, and Richard L. Mansell. "Naringin Levels in Citrus Tissues." Plant Physiology 77, no. 4 (April 1, 1985): 896–902. http://dx.doi.org/10.1104/pp.77.4.896.

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31

Jourdan, Pablo S., Cecilia A. McIntosh, and Richard L. Mansell. "Naringin Levels in Citrus Tissues." Plant Physiology 77, no. 4 (April 1, 1985): 903–8. http://dx.doi.org/10.1104/pp.77.4.903.

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32

Gmitter, Frederick G., and Xubai Ling. "Embryogenesis in Vitro and Nonchimeric Tetraploid Plant Recovery from Undeveloped Citrus Ovules Treated with Colchicine." Journal of the American Society for Horticultural Science 116, no. 2 (March 1991): 317–21. http://dx.doi.org/10.21273/jashs.116.2.317.

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A method was developed to produce nonchimeric, autotetraploid Citrus plants via in vitro somatic embryogenesis in the presence of colchicine. Undeveloped ovules from immature fruit of `Valencia' sweet orange (Citrus sinensis [L.] Osb.) and `Orlando' and `Minneola' tangelos (Citrus reticulata Blanco × Citrus × paradisi Macf.) were held on Murashige and Tucker medium with 500 mg malt extract/liter and 0.0090, 0.01%, or 0.10% colchicine for 21 days. Embryogenesis from tangelo ovules was suppressed by 0.10% colchicine, but no such effect was observed among sweet orange ovules. Colchicine treatment
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33

MATSUURA, Yasushi. "Limit to the deesterification of citrus pectin by citrus pectinesterase." Agricultural and Biological Chemistry 51, no. 6 (1987): 1675–77. http://dx.doi.org/10.1271/bbb1961.51.1675.

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34

Stover, Ed, Richard R. Stange, T. Gregory McCollum, Jesse Jaynes, Michael Irey, and Erik Mirkov. "Screening Antimicrobial Peptides In Vitro for Use in Developing Transgenic Citrus Resistant to Huanglongbing and Citrus Canker." Journal of the American Society for Horticultural Science 138, no. 2 (March 2013): 142–48. http://dx.doi.org/10.21273/jashs.138.2.142.

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Huanglongbing {HLB [associated with Candidatus Liberibacter sp. (CLas)]} and asiatic citrus canker {ACC [causal organism Xanthomonas citri ssp. citri (XCC)]} are bacterial diseases that seriously threaten sustainability of the Florida citrus (Citrus sp.) industry. Sweet orange (Citrus sinensis) and grapefruit (Citrus paradisi) are highly susceptible to ACC and improvement through conventional breeding is a long-term process, making transgenic solutions attractive. No strong HLB resistance has been identified within cultivated citrus scion types: creation of transgenic citrus that would permit
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35

Nolte, Kurt D., Andrew D. Hanson, and Douglas A. Gage. "Proline Accumulation and Methylation to Proline Betaine in Citrus: Implications for Genetic Engineering of Stress Resistance." Journal of the American Society for Horticultural Science 122, no. 1 (January 1997): 8–13. http://dx.doi.org/10.21273/jashs.122.1.8.

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Proline and various betaines can function as osmoprotectants and cryoprotectants when accumulated in the cytoplasm of cells. Genetic engineering can raise levels of these compounds and thereby improve stress resistance; Citrus species are potential candidates for this. Before attempting such engineering, it is necessary to characterize the natural osmoprotectants of Citrus and related genera. We therefore surveyed 55 cultivated and wild species of the Aurantioideae, analyzing proline and betaines in leaves of mature trees. Some citrus relatives accumulated proline alone; others accumulated pro
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36

Oliveira, Roberto Pedroso de, Carlos Ivan Aguilar-Vildoso, and Marcos Antônio Machado. "Selection processes in a citrus hybrid population using RAPD markers." Pesquisa Agropecuária Brasileira 38, no. 11 (November 2003): 1297–302. http://dx.doi.org/10.1590/s0100-204x2003001100007.

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The objective of this work was to evaluate the processes of selection in a citrus hybrid population using segregation analysis of RAPD markers. The segregation of 123 RAPD markers between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck) was analysed in a F1 progeny of 94 hybrids. Genetic composition, diversity, heterozygosity, differences in chromosomal structure and the presence of deleterious recessive genes are discussed based on the segregation ratios obtained. A high percentage of markers had a skeweness of the 1:1 expected segregation ratio in
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37

Chaturvedi, H. C., and A. K. Sharma. "Androgenesis in Citrus aurantifolia (Christm.) swingle." Planta 165, no. 1 (1985): 142–44. http://dx.doi.org/10.1007/bf00392223.

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38

Trainin, Taly, Alexander Lipsky, Avraham A. Levy, and Doron Holland. "Prolonged Somatic Transposition in Citrus: The Autonomous Ac Transposable Element Remains Active in the Citrus Genome for Several Years." Journal of the American Society for Horticultural Science 130, no. 1 (January 2005): 95–101. http://dx.doi.org/10.21273/jashs.130.1.95.

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The maize transposable element Activator (Ac) has been shown to be active in a number of dicots, including arabidopsis [Arabidopsis thaliana (L.) Heynh.], tobacco (Nicotiana tabacum L.), tomato (Lycopersicon esculentum Mill.), potato (Solanum tuberosum L.), and aspen (Populus tremuloides Michx.). However, no information is available on somatic transposition in any plant during several years of growth and development. It is not known how transposition affects genetic variability among vegetative parts that have developed during a long period of growth. In order to explore the possibility of usi
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39

Tan, Fui-Ching, and Stephen M. Swain. "Functional characterization of AP3, SOC1 and WUS homologues from citrus (Citrus sinensis)." Physiologia Plantarum 131, no. 3 (November 2007): 481–95. http://dx.doi.org/10.1111/j.1399-3054.2007.00971.x.

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40

Hartmond, Ulrich, Rongcai Yuan, Jacqueline K. Burns, Angela Grant, and Walter J. Kender. "Citrus Fruit Abscission Induced by Methyl-jasmonate." Journal of the American Society for Horticultural Science 125, no. 5 (September 2000): 547–52. http://dx.doi.org/10.21273/jashs.125.5.547.

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Methyl jasmonate (MJ) was tested as a potential abscission chemical to enhance mechanical harvest of `Hamlin' and `Valenica' orange [Citrus sinensis (L.) Osb.]. In field experiments, a solution of 1, 5, 10, 20, or 100 mm MJ was applied either as a stem wrap to individual fruit or as a spray to entire trees or canopy sectors. Solutions of 10, 20, and 100 mm MJ resulted in significant and consistent reduction of fruit detachment force and caused fruit drop within 7 to 10 days. Fruit loosening was preceded by an increase in the internal ethylene concentration of fruit similar to that of other exp
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41

Louzada, E. S., H. S. del Rio, D. Xia, and J. M. Moran-Mirabal. "Preparation and Fusion of Citrus sp. Microprotoplasts." Journal of the American Society for Horticultural Science 127, no. 4 (July 2002): 484–88. http://dx.doi.org/10.21273/jashs.127.4.484.

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Large-scale production of microprotoplasts from `Ruby Red' grapefruit (Citrus paradisi Macf.) and from the Citrus L. sp. relative Swinglea glutinosa (Blanco) Merr., was performed after treatment of suspension cells with APM. An average of 75.2% of the microprotoplasts contained a single chromosome, followed by 17.1% with two, 4.6% with three, and 2.0% with four. Only 1.1% had more than five chromosomes. Maximum chromosome number observed was eight and the average yield was 2 × 106 of total microprotoplasts per gram of suspension cells. Flow cytometry analysis confirmed low DNA content. The pol
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42

Maust, B. E., and J. G. Williamson. "Nitrogen Nutrition of Containerized Citrus Nursery Plants." Journal of the American Society for Horticultural Science 119, no. 2 (March 1994): 195–201. http://dx.doi.org/10.21273/jashs.119.2.195.

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Experiments were conducted with `Hamlin' orange [Citrus sinensis (L.) Osb.] budded on Cleopatra mandarin (Citrus reticulata Blanco) or Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] seedling rootstocks to determine minimum container solution N concentrations required for optimum growth and fertilizer uptake efficiency at various growth stages. Plants were fertigated daily with 1 liter of N solution at either 0, 12.5, 25, 50, 100, or 200 mg·liter-1 from NH4NO3 or 0, 3.13, 6.25, 12.5, 25, or 50 mg·liter-1 from NH4NO3 dissolved in a complete nutrient solution, respec
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43

Garcia-Luis, A., F. Fornes, and J. L. Guardiola. "Leaf Carbohydrates and Flower Formation in Citrus." Journal of the American Society for Horticultural Science 120, no. 2 (March 1995): 222–27. http://dx.doi.org/10.21273/jashs.120.2.222.

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The carbohydrate contents of the leaves of satsuma mandarin (Citrus unshiu Marc.) trees were altered before or during the low temperature flower induction period to determine the relationship between gross levels of carbohydrates and flower formation. Early removal of the fruit and girdling of the branches on either fruiting or defruited trees caused an accumulation of carbohydrates in the leaves and increased flower formation. Shading the trees resulted in a transient reduction in leaf carbohydrate levels and in a decrease in flower formation. Although a relationship between carbohydrate leve
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44

Petracek, Peter D., D. Frank Kelsey, and Craig Davis. "Response of Citrus Fruit to High-pressure Washing." Journal of the American Society for Horticultural Science 123, no. 4 (July 1998): 661–67. http://dx.doi.org/10.21273/jashs.123.4.661.

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The effect of high-pressure washing (HPW) on the surface morphology and physiology of citrus fruit was examined. Mature white (Citrus paradisi Macf. `Marsh') and red (Citrus paradisi Macf. `Ruby Red') grapefruit, oranges (Citrus sinensis L. `Hamlin'), and tangelos (Citrus reticulata Blanco × Citrus paradisi Macf. `Orlando') were washed on a roller brush bed and under a water spraying system for which water pressure was varied. Washing white grapefruit and oranges for 10 seconds under conventional low water pressure (345 kPa at cone nozzle) had little effect on peel wax fine structure. Washing
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45

Fang, D. Q., C. T. Federici, and M. L. Roose. "A High-Resolution Linkage Map of the Citrus Tristeza Virus Resistance Gene Region in Poncirus trifoliata (L.) Raf." Genetics 150, no. 2 (October 1, 1998): 883–90. http://dx.doi.org/10.1093/genetics/150.2.883.

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Abstract Resistance to citrus tristeza virus (CTV) was evaluated in 554 progeny of 10 populations derived from Poncirus trifoliata. A dominant gene (Ctv) controlled CTV resistance in P. trifoliata. Twenty-one dominant PCR-based DNA markers were identified as linked to Ctv by bulked segregant analysis. Of the 11 closest markers to Ctv, only 2 segregated in all populations. Ten of these markers were cloned and sequenced, and codominant RFLP markers were developed. Seven RFLP markers were then evaluated in 10 populations. Marker orders were consistent in all linkage maps based on data of single p
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46

Killiny, Nabil, Faraj Hijaz, Scott J. Harper, and William O. Dawson. "Effects of Citrus tristeza closterovirus infection on phloem sap and released volatile organic compounds in Citrus macrophylla." Physiological and Molecular Plant Pathology 98 (April 2017): 25–36. http://dx.doi.org/10.1016/j.pmpp.2017.03.003.

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47

Dentali, Steven J. "Comment on Citrus aurantium Minireview." Experimental Biology and Medicine 230, no. 2 (February 2005): 102. http://dx.doi.org/10.1177/153537020523000203.

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48

Distefano, Gaetano, Giuseppina Las Casas, Stefano La Malfa, Alessandra Gentile, Eugenio Tribulato, and Maria Herrero. "Pollen Tube Behavior in Different Mandarin Hybrids." Journal of the American Society for Horticultural Science 134, no. 6 (November 2009): 583–88. http://dx.doi.org/10.21273/jashs.134.6.583.

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Seedlessness is an important trait in the evaluation of commercial mandarin for fresh consumption. However, in the last decade, the presence of seeds in fruit of cultivars considered as seedless has become a problem in different citrus-growing areas because the commercial value is depreciated. Seeds have appeared concomitantly with the introduction of new cultivars that appear to be cross-compatible. To overcome this problem, different strategies have been explored, but a definitive answer is still elusive. The search for alternatives contrasts with how little is known about the basis of the p
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49

Kijas, J. M. H., J. C. S. Fowler, and M. R. Thomas. "An evaluation of sequence tagged microsatellite site markers for genetic analysis within Citrus and related species." Genome 38, no. 2 (April 1, 1995): 349–55. http://dx.doi.org/10.1139/g95-045.

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Microsatellites, also called sequence tagged microsatellite sites (STMSs), have become important markers for genome analysis but are currently little studied in plants. To assess the value of STMSs for analysis within the Citrus plant species, two example STMSs were isolated from an intergeneric cross between rangpur lime (Citrus × limonia Osbeck) and trifoliate orange (Poncirus trifoliata (L.) Raf.). Unique flanking primers were constructed for polymerase chain reaction amplification both within the test cross and across a broad range of citrus and related species. Both loci showed length var
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50

Duhan, Naveen, Manish Meshram, Cristian D. Loaiza, and Rakesh Kaundal. "citSATdb: Genome-Wide Simple Sequence Repeat (SSR) Marker Database of Citrus Species for Germplasm Characterization and Crop Improvement." Genes 11, no. 12 (December 10, 2020): 1486. http://dx.doi.org/10.3390/genes11121486.

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Microsatellites or simple sequence repeats (SSRs) are popular co-dominant markers that play an important role in crop improvement. To enhance genomic resources in general horticulture, we identified SSRs in the genomes of eight citrus species and characterized their frequency and distribution in different genomic regions. Citrus is the world’s most widely cultivated fruit crop. We have implemented a microsatellite database, citSATdb, having the highest number (~1,296,500) of putative SSR markers from the genus Citrus, represented by eight species. The database is based on a three-tier approach
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