Tesis sobre el tema "Cellular RNA"
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Hunt, Sarah Louise. "Cellular proteins required for rhinovirus RNA translation". Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313880.
Texto completoChan, Annie Yee-Man. "Interactions between the influenza virus RNA polymerase and cellular RNA polymerase II". Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670083.
Texto completoBailey, Daniel John. "Cellular proteins in picornavirus replication". Thesis, University of Reading, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298484.
Texto completoVasale, Jessica J. "Roles of Cellular RNA-Dependent RNA Polymerases in Endogenous Small RNA Pathways in Caenorhabditis elegans: A Dissertation". eScholarship@UMMS, 2010. https://escholarship.umassmed.edu/gsbs_diss/481.
Texto completoOsborn, Maire. "Cellular RNA Targeting by Platinum (II) Anticancer Therapeutics". Thesis, University of Oregon, 2014. http://hdl.handle.net/1794/17920.
Texto completoBrown, E. C. "Cellular proteins involved in translation of human rhinovirus RNA". Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596963.
Texto completoTodorova, Tanya (Tanya Todorova). "Function and regulation of PARP13 binding to cellular RNA". Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/97789.
Texto completoCataloged from PDF version of thesis. Vita.
Includes bibliographical references.
Poly(ADP-ribose) polymerase-13 (PARP13) is a member of the PARP family of proteins - enzymes that use NAD+ to synthesize a posttranslational protein modification called poly(ADP-ribose) (PAR). PARPs function in multiple cellular pathways, and recently several members of the family have been implicated in regulating various steps in RNA metabolism, from splicing to translation and decay. PARP1 3 is the best-understood RNA-regulatory PARP. Initially discovered as a host immune factor, PARP13 functions by binding viral transcripts via its four CCCH-type zinc fingers and targeting them for degradation. In the context of the immune response PARP1 3 can also inhibit the translation of its viral targets and enhance the activity of other RNA-binding viral receptors, such as RIG-1. More recently PARP13 was shown to also indirectly regulate the cellular transcriptome by inhibiting the activity of Argonaute 2 (Ago2), a member of the miRNA silencing pathway. While itself catalytically inactive, PARP13 is modified by PAR and can target Ago2 for modification by a yet unknown PARP. However, it remains unclear if RNA binding is required for this function of PARP1 3. Indeed, even though multiple viruses are known to be restricted by PARP13, cellular mRNA targets of PARP13 binding and regulation have not yet been identified. Here we show that PARP1 3 binds endogenous RNA and regulates the cellular transcriptome. We identify TRAILR4 mRNA as the first cellular target of PARP13 regulation and demonstrate that PARP13 represses TRAILR4 expression posttranscriptionally by binding to a specific region in the 3' untranslated region of the transcript and targeting it for degradation in a primarily 3'-5' decay mechanism. By inhibiting the expression of TRAILR4 - a decoy pro-survival receptor of the apoptotic ligand TRAIL, PARP1 3 regulates the cellular response to TRAIL and acts as a pro-apoptotic factor. We also examine possible mechanisms of regulation of PARP1 3 function. We identify the RNA-helicase DHX30 as a constitutive PARP1 3-interacting protein and show that the two proteins co-regulate a subset of cellular transcripts. We further demonstrate that the PAR-binding domain of PARP1 3 inhibits RNA binding, while PARP1 3 interaction with PARP5a and covalent modification with PAR appear to be mutually exclusive with RNA binding.
by Tanya Todorova.
Ph. D.
Mullani, Nowsheen. "An RNA Signature Links Oxidative Stress To Cellular Senescence". Electronic Thesis or Diss., Sorbonne université, 2019. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2019SORUS560.pdf.
Texto completoOxidative Stress is one of the routes leading to cellular senescence. While the damages that reactive oxygen species inflict on proteins and DNA are well described, our insight on how transcription may participate in the onset of senescence is still limited. At a transcriptional level, oxidative stress results in accumulation of promoter RNAs (uaRNAs) and enhancer RNAs (eRNAs) as a consequence of defective release of the RNAPII from the chromatin a phenomenon known as RNAPII crawling. We observed that RNAPII crawling was also detected downstream of a small series of genes known to be regulated by HP1Υ at the level of their termination. Exploring this phenomenon yielded an unexpected result in the sense that it revealed an inhibiting effect of hydrogen peroxide on the RNA exosome complex involved in degradation of polyadenylated RNAs. The crawling RNAPII results in the transcription of ALU sequences located in the neighborhood of promoters and enhancers and downstream of intron-less genes and of small series of intron-containing genes. As ALU sequences contain genome encoded A tracts, they should normally be degraded by the RNA exosome. Yet, as oxidative stress also inhibits this RNAse activity, mRNAs containing serendipitously transcribed ALU sequences get stabilized and are detected in the cytoplasm and even polysome fractions. This phenomenon may participate in the onset of the interferon response associated with oxidative stress
Stassinopoulos, Ioannis A. "Interactions of picornavirus internal ribosome entry sites with cellular proteins". Thesis, University of Sussex, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322940.
Texto completoSchmier, Brad J. "The Molecular Machinery Critical to the Degradation of Cellular RNA". Scholarly Repository, 2012. http://scholarlyrepository.miami.edu/oa_dissertations/714.
Texto completoTedeschi, Frank A. Tedeschi. "IDENTIFICATION OF CELLULAR RNA BINDING SITES OF DEAD-BOX HELICASES". Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1531217057171378.
Texto completoIdris, Jalilah. "Investigating novel roles of RNA binding proteins SAFB1 and SAFB2 in RNA processing and in cellular stress". Thesis, University of Bristol, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.738206.
Texto completoCordiner, Ross Andrew Alex. "The cellular functions of the microprocessor complex". Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25877.
Texto completoOzes, Ali Rayet. "Targeting the long non coding RNA HOTAIR in cancer". Thesis, Indiana University, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10154781.
Texto completoOvarian cancer (OC) takes the lives of nearly 14,000 US women every year. Although platinum is one of the most effective drugs in treating ovarian cancer, the development of platinum resistance is one of the biggest challenges facing patients. I have shown that the long non-coding RNA HOTAIR contributes to platinum-resistant OC and determined the regulators and targets of HOTAIR during the platinum-induced DNA damage response. My published data supports the role of HOTAIR in contributing to DNA damage induced cellular senescence and secretion of pro-inflammatory cytokines leading to cisplatin resistance. My unpublished work (under review) analyzed the interaction of HOTAIR with the PRC2, its known interacting partner. In this study, I developed a novel strategy blocking HOTAIR-PRC2 interaction and resensitized ovarian tumors to platinum in mouse studies. The results offer a pre-clinical proof of concept for targeting long non-coding RNAs as a therapeutic approach and may represent a strategy to overcome chemotherapy resistance in tumors exhibiting high expression of HOTAIR, a frequent observation in high grade serous OC.
Babendure, Jeremy R. "Utilizing RNA structure as a tool in molecular and cellular biology". Diss., Connected to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2005. http://wwwlib.umi.com/cr/ucsd/fullcit?p3190000.
Texto completoTitle from first page of PDF file (viewed Mar. 6, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 122-131).
Nurmohamed, Salima. "Communication between the Escherichia coli RNA degradative machineries and cellular metabolism". Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611286.
Texto completoÖhrmalm, Christina. "Functional characterization of the cellular protein p32 : a protein regulating adenovirus transcription and splicing through targeting of phosphorylation /". Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6794.
Texto completoMcNally, Beth Anne. "A role for cytoplasmic PML in the cellular antiviral response". Columbus, Ohio : Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1133377007.
Texto completoYork, Ashley D. "A study of viral and cellular factors in the regulation of the influenza virus RNA-dependent RNA polymerase". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:5958fafd-4c91-4434-910e-29e2dd0539b9.
Texto completoJeyaraj, Selvi Chrysolyte. "A role for the mRNA-stabilizing protein HuR in protection from cellular ATP depletion". Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1186773861.
Texto completoWong, Tsz-lo y 黃子璐. "Cellular role of miR-143 in cervical cancer". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48274045.
Texto completopublished_or_final_version
Pathology
Master
Master of Medical Sciences
Schonauer, Melissa. "Intersection of RNA Processing and Fatty Acid Synthesis and Attachment in Yeast Mitochondria". Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/194674.
Texto completoMahmoudi, Massoud. "Induction of Interferon Messenger RNA and Expression of Cellular Oncogenes in Human Lymphoblastoid Cells". Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc798199/.
Texto completoMina, Ibarra Leonardo Bruno. "Cellular mRNA decay factors involved in the hepatitis C virus life cycle". Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7215.
Texto completoSince all (+)RNA viruses need to regulate the exit of the viral genome from the cellular translation machinery to replication and these proteins are conserved from yeast to humans, We hypothesized that the human homologues of Lsm1-7/Dhh1/Pat1 are required for the replication of human (+) RNA viruses. In this work we proved this hypothesis by showing that HCV translation and replication depend on these cellular proteins. Moreover, the requirement of these factors for efficient HCV RNA translation was linked exclusively to the 5´ and 3´ nontranslated regions (NTRs) of the viral genome. Furthermore, the LSm1-7 complex specifically interacts in vitro with essential cis-acting HCV RNA elements located in the NTRs.
El grupo de virus de RNA de cadena de polaridad positiva ((+)RNA) incluye numerosos patógenos de plantas, animales y humanos, tales como el virus de la Hepatitis C (VHC). Sus genomas virales imitan a los RNAm celulares, sin embargo, además de actuar como mensajeros para la traducción de proteínas virales, también actúan como moldes para la replicación viral. Debido a que estas dos funciones son mutuamente excluyentes, un paso clave en la replicación de todos los virus (+) RNA es la salida regulada del RNA genómico desde la maquinaria de traducción celular hacia las complejos de replicación virales. Utilizando un sistema modelo que permite la replicación del virus del mosaico del bromo (VMB), un virus (+) RNA de plantas, en levaduras, nuestro grupo ha demostrado que los activadores de decapping celulares Dhh1, LSm1 y Pat1 juegan un papel clave en este paso. La proteína LSm1 es una subunidad del complejo celular LSm1-7. Hemos demostrado recientemente que dichos complejos interactúan directamente con secuencias específicas en el genoma del VMB y que dichas interacciones regulan la traducción y replicación del VMB. De manera interesante, la proteína homologa de LSm1 en bacterias, Hfq, es necesaria para la replicación del bacteriófago (+) RNA Qβ en E. coli.
Debido a que todos los virus (+) RNA necesitan regular la salida del genoma viral desde la maquinaria traduccional de la célula hacia los complejos de replicación virales, y que las proteínas Dhh1, LSm1-7 y Pat1 están conservadas de levaduras a humanos, nuestra hipótesis es que lo homólogos humanos de Lsm1-7/Dhh1/Pat1 son requeridos para la replicación de virus (+)RNA de humanos. En este trabajo, hemos demostrado esta hipótesis mostrando que la traducción y la replicación del virus de la hepatitis C (VHC) dependen de estas proteínas celulares. Más aún, el requerimiento de estos factores para una eficiente traducción del genoma del VHC está vinculado de manera exclusiva a las regiones no traducidas (RNT) 5´ y 3´ del genome viral. Además, el complejo cellular LSm1-7 interactúa in vitro de manera específica con elementos esenciales del RNA del VHC que actúan en cis localizados en las RNTs.
Plakos, Kory. "Platinum-seq: High-throughput mapping of small-molecule platinum adducts on cellular RNA". Thesis, University of Oregon, 2017. http://hdl.handle.net/1794/22269.
Texto completoChambers, A. "RNA 3' cleavage and polyadenylation in oocytes, eggs and embryos of Xenopus laevis". Thesis, University of Warwick, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380275.
Texto completoRubilar, Guzman Paulina. "Cellular host factors involved in the translation of the HIV-1 genomic RNA". Thesis, Lyon, École normale supérieure, 2015. http://www.theses.fr/2015ENSL1009/document.
Texto completoHuman Immunodeficiency virus type 1 (HIV-1) is a positive strand RNA virus belonging to the lentivirus genus of the retroviridae family and it is the etiological agent of the pandemic AIDS, which is a major health concern worldwide. Throughout HIV-1 replication cycle, the production of viral proteins depends exclusively on the cellular translational machinery. This is the reason why we have explored the role of some cellular factors that could control HIV-1 translation at different stages. We have focused our studies on the translation of the full length genomic RNA (gRNA), which serves both as genome for viral encapsidation and as a messenger for translation of Gag and Gag-Pol viral polyproteins.1) The role of the RNA helicase DDX3 in HIV-1 translation Initiation The fact that HIV-1 possesses a highly structured 5’ untranslated region (5’UTR) prompted us to speculate that DDX3 may be involved in HIV-1 translation. We used a combination of in vitro and ex-vivo approaches to show that DDX3 was able to bind and form complexes with the 5’-UTR of HIV-1 to assist translation initiation. We also demonstrated that DDX3 can form a complex with initiation factors such as PABP, eIF4G and eIF4E. 2) Programmed Ribosomal Frameshift (PRF) in the genomic RNA of HIV-1Translation of HIV-1 Gag-Pol polyprotein requires a -1 PRF. This mechanism allows the synthesis of Gag and Gag-Pol polyproteins, using the same mRNA template, at ratios of 95 and 5% respectively. Keeping the -1PRF ratio is important as any change leads to reduction in virus infectivity.By means of a dual reporter construct and full provirus replication system we were able to demonstrate that the stress granules associated protein TIAR, controls HIV-1 infectious progeny by regulating the ratio of the HIV-1 PRF
Win, Maung Nyan Parker Carl Stevens Smolke Christina D. "Engineering RNA devices for gene regulation, biosensing, and higher-order cellular information processing /". Diss., Pasadena, Calif. : Caltech, 2008. http://resolver.caltech.edu/CaltechETD:etd-05282008-142750.
Texto completoPalsule, Geeta. "Mechanism and Functional Consequences of Generating and Processing Drosophila RNase P RNA from an Intron". The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555628225881656.
Texto completoKheimar, Ahmed Mahmoud Osman [Verfasser]. "Tumor promoting functions of cellular telomerase RNA and viral RNAs in herpesvirus-induced cancer formation / Ahmed Mahmoud Osman Kheimar". Berlin : Freie Universität Berlin, 2017. http://d-nb.info/1147758247/34.
Texto completoOstertag, Derek Glenn. "Novel dsRNA-dependent activation of a cellular antiviral response to vesicular stomatitis virus /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2005. http://wwwlib.umi.com/cr/ucsd/fullcit?p3167840.
Texto completoChilds, April Celeste. "The Effects of EIF5A and of the Polyamines on RNA Processing, Translation, and P-Body Formation". Diss., Tucson, Ariz. : University of Arizona, 2005. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu%5Fetd%5F1326%5F1%5Fm.pdf&type=application/pdf.
Texto completoGuo, Bing. "Positive correlation between cellular levels of storage compounds and RNA in activated sludge bacteria". Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=107874.
Texto completoPour mettre en œuvre des solutions rationnelles aux problèmes de la séparation des solides tels que la prolifération de bactéries filamenteuses causant le foisonnement et le moussage et afin de concevoir rationnellement de nouveaux procédés pour la production de produits à valeur ajoutée, il est nécessaire de comprendre les fonctions écologiques et la dynamique des populations de bactéries hétérotrophes qui résident dans les installations de traitement des eaux usées par boues activées. Bien que la caractérisation phylogénétique (baseée sur l'ADN et l'ARN) de ces communautés microbiennes ait démontré leur grande diversité, les liens entre les populations hétérotrophes et des niches fonctionnelles spécifiques sont encore ténus. Parce que les niveaux d'ARN sont liés aux taux de croissance et que les composés de stockage sont accumulés en réponse à des dynamiques de croissance spécifiques, l'abondance de ces constituants cellulaires peut servir de marqueurs biologiques fonctionnels. Une méthode de cytométrie en flux a été développée pour les mesurer sur une base cellule par cellule en utilisant des colorants fluorescents : RNASelect pour l'ARN, le rouge du nil pour les polyhydroxyalcanoates (PHA) et le 7-AAD pour l'ADN. La méthode a été validée contre cinq souches représentant un large éventail de diversité bactérienne : Escherichia coli K-12, Rhodococcus jostii RHA1, Bacillus subtilis, Cupriavidus necator DSM428 and DSM 541 (une souche mutante de DSM428 incapable d'accumuler des PHA). La comparaison des ratios ARN/ADN déterminés par cytométrie en flux et par extraction d'acide nucléique entre ces souches a démontré une corrélation générale acceptable.La méthode a ensuite été utilisée pour étudier les échantillons obtenus à partir de trois réacteurs par boues activées indépendants situés dans une usine de traitement des eaux usées près de Montréal : un réacteur à pleine échelle, un réacteur à l'échelle pilote utilisant de l'ozone pour réduire la production de boues, et un réacteur de contrôle à l'échelle pilote. Dans tous les cas, on a constaté qu'un niveau élevé des ratios des signaux fluorescents d'ARN/ADN était corrélé avec des signaux élevés de fluorescence associée au PHA. Par ailleurs, lorsque les signaux associés au PHA de la biomasse échantillonnée à partir des réacteurs à l'échelle pilote ont été comparés, on a constaté que le signal PHA était plus élevé pour les échantillons du réacteur traité à l'ozone. L'augmentation du PHA résulte probablement de la production de substrats supplémentaires facilement dégradables par le traitement par ozone des boues. Nous suggérons que cette corrélation entre les ratios d'ARN/ADN et l'abondance de PHA représente une caractérisation fonctionnelle des hétérotrophes. Les populations consommant des substrats facilement dégradables auraient un ratio ARN/ADN élevé et elles accumulent des composés de stockage, tandis que ce serait l'inverse pour les populations consommant des substrats lentement dégradables.
Wang, Dong 1975. "Cisplatin-induced nucleosome and RNA polymerase II modification mediate cellular response to the drug". Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/28693.
Texto completoVita.
Includes bibliographical references.
(cont.) with recombinant material. The in vitro system established in this study will facilitate the investigation of platinum-DNA damage by DNA repair processes and help elucidate the role of specific post-translational modification in NER of platinum-DNA adducts at the physiologically relevant nucleosome level. Chapter 3: Nucleotide Excision Repair of Site-Specifically Platinum-Modified Tetrasomes. The nucleosome, the basic structural unit of chromatin, is composed of a histone (H3/H4)₂ tetramer flanked by two H2A/H2B dimers, around which is wrapped 146 base pairs (bp) of DNA. The (H3/H4)₂ tetramer plays a central role in the structural integrity and positioning of the nucleosome core particle. Site- specifically platinated tetrasomes were prepared to investigate the modulating effects of histone tetramers on excision repair of cisplatin-intrastrand cross-links. In addition, (Pt(DACH))²⁺-modified tetrasome was prepared to investigate the effect of spectator ligands on excision repair of platinated tetrasomes. The NER results reveal that the (H3/H4)₂ tetramer is sufficient to block excision of both cisplatin-DNA and Pt(DACH))²⁺-DNA adduct in vitro. The efficiency of excision of cisplatin-modified tetrasomal DNA is about half (53%) that of the [Pt(DACH)]²⁺-modified tetrasome. Chapter 4: Cisplatin Adducts Change the Rotational Positioning of DNA on the Nucleosome ...
Chapter 1: Cellular Processing of Platinum Anti-Cancer Drugs --Identifying Pathways for Chemogenotherapeutic Drug Design. Cisplatin, carboplatin, and oxaliplatin are widely used in cancer chemotherapy. Platinum-DNA adducts, formed following uptake of the drug into the nucleus of cells, activate several cellular processes that mediate the cytotoxicity of these platinum drugs. This review focuses on recently discovered cellular pathways that are activated in response to cisplatin, including those involved in regulating drug uptake, the signaling of DNA damage, cell cycle checkpoints and arrest, DNA repair, and cell death. Such knowledge of the cellular processing of cisplatin adducts with DNA provides valuable clues for the rational design of more efficient platinum-based drugs as well as the development of new therapeutic strategies. Chapter 2: Nucleotide Excision Repair from a Site-Specifically Platinum-Modified Nucleosome. Nucleotide excision repair is a major cellular defense mechanism against the toxic effects of the anticancer drug cisplatin and other platinum based chemotherapeutic agents. In this study, mononucleosomes were prepared containing either a d(GpG) or a d(GpTpG) intrastrand cross-link. Comparison of the extent of repair by mammalian cell extracts of free and nucleosomal DNA containing the same platinum-DNA adduct reveals that the nucleosome significantly inhibits nucleotide excision repair. The effects of post-translational modification of histones on excision of platinum damage from nucleosomes were investigated by comparing native and recombinant nucleosomes containing the same intrastrand d(GpTpG) cross-link. Excision from native nucleosomal DNA is [approximately] 2-fold higher than the level observed
by Dong Wang.
Ph.D.
Ribeiro, Diogo. "Discovery of the role of protein-RNA interactions in protein multifunctionality and cellular complexity". Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0449/document.
Texto completoOver time, life has evolved to produce remarkably complex organisms. To cope with this complexity, organisms have evolved a plethora of regulatory mechanisms. For instance, thousands of long non-coding RNAs (lncRNAs) are transcribed by mammalian genomes, presumably expanding their regulatory capacity. An emerging concept is that lncRNAs can serve as protein scaffolds, bringing proteins in proximity, but the prevalence of this mechanism is yet to be demonstrated. In addition, for every messenger RNA encoding a protein, regulatory 3’ untranslated regions (3’UTRs) are also present. Recently, 3’UTRs were shown to form protein complexes during translation, affecting the function of the protein under synthesis. However, the extent and importance of these 3’UTR-protein complexes in cells remains to be assessed.This thesis aims to systematically discover and provide insights into two ill-known regulatory mechanisms involving the non-coding portion of the human transcriptome. Concretely, the assembly of protein complexes promoted by lncRNAs and 3’UTRs is investigated using large-scale datasets of protein-protein and protein-RNA interactions. This enabled to (i) predict hundreds of lncRNAs as possible scaffolding molecules for more than half of the known protein complexes, as well as (ii) infer more than a thousand distinct 3’UTR-protein complexes, including cases likely to post-translationally regulate moonlighting proteins, proteins that perform multiple unrelated functions. These results indicate that a high proportion of lncRNAs and 3’UTRs may be employed in regulating protein function, potentially playing a role both as regulators and as components of complexity
Saini, Harleen. "Intron and Small RNA Localization in Mammalian Neurons". eScholarship@UMMS, 2019. https://escholarship.umassmed.edu/gsbs_diss/1044.
Texto completoSvensson, Valentine. "Probabilistic modelling of cellular development from single-cell gene expression". Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267937.
Texto completoKolisnichenko, Marina. "The role of the polyadenylation site of the melanocortin 1 receptor in generating MC1R-TUBB3 chimeras and attenuation of TORC1 delays the onset of replicative and RAS-induced cellular senescience". Thesis, University of Oxford, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711654.
Texto completoCooper, Samuel J. "Proteomic investigation of factors binding Gurken RNA in Drosphila and Hepatitis C virus cellular interactions". Thesis, University of Oxford, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540148.
Texto completoBurns, David M. "Post-Transcriptional Control of Human Cellular Senescence: A Dissertation". eScholarship@UMMS, 2010. https://escholarship.umassmed.edu/gsbs_diss/491.
Texto completoSmith, Gregory Robert. "Unraveling the Role of Cellular Factors in Viral Capsid Formation". Research Showcase @ CMU, 2015. http://repository.cmu.edu/dissertations/475.
Texto completoZhang, Wei. "Functional elucidation of BS69 /". View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?BICH%202008%20ZHANG.
Texto completoBryant, Helen Elizabeth. "Analysis of cellular and viral proteins that interact with the IE63 protein of herpes simplex virus type 1". Thesis, University of Glasgow, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312135.
Texto completoZangl, Maximiliane Sophie [Verfasser]. "Influence of Viral Proteins and Cellular Exoribonuclease Xrn1 on RNA Recombination in Pestiviruses / Maximiliane Sophie Zangl". Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2015. http://d-nb.info/1073954560/34.
Texto completoVreeland, Amanda C. "Cellular Retinoic Acid-Binding Protein 2 Cooperates with HuR to Stabilize RNA and Inhibit Tumor Growth". Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1409933949.
Texto completoXu, Kai. "KEY ROLES OF SUB-CELLULAR MEMBRANES AND CO-CHAPERONE IN TOMBUSVIRUS REPLICATION". UKnowledge, 2014. http://uknowledge.uky.edu/plantpath_etds/15.
Texto completoTenOever, Benjamin R. "Cellular recognition of RNA virus infection leading to activation of interferon regulatory factors three and seven and establishment of the antiviral state". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84849.
Texto completoNitin, Nitin. "Optical and MR Molecular Imaging Probes and Peptide-based Cellular Delivery for RNA Detection in Living Cells". Diss., Available online, Georgia Institute of Technology, 2005, 2005. http://etd.gatech.edu/theses/available/etd-08102005-120350/.
Texto completoDr. X. Hu, Committee Member ; Dr. Al Merrill, Committee Member ; Dr. Niren Murthy, Committee Member ; Dr. Gang Bao, Committee Chair ; Dr. Nicholas Hud, Committee Member. Includes bibliographical references.
Galão, Rui Pedro Ribeiro. "Role of the cellular decapping activator LSM1-7 complex in the replication of positive-strand RNA viruses". Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7222.
Texto completoUtilizando la capacidad del BMV, un virus de ARN de cadena positiva (ARN(+)), para replicar en levaduras se ha demostrado previamente que las subunidades del anillo LSm1-7, así como Pat1 y Dhh1, desempeñan un papel esencial en la transición del genoma del virus de BMV desde traducción a replicación. En células no infectadas, estas proteínas median la transición de ARNm celulares de la traducción a un estado de no-traducción mediante la activación del proceso de decapping en la via 5'-3' de degradación de los ARNs celulares dependiente de deadenilación. Teniendo en cuenta la conservación de esta vía desde levaduras a humanos y la necesidad común de todos los virus ARN(+) para regular la transición de sus genomas desde un estado activo de traducción a otro no activo para permitir la replicación, una posibilidad interesante, y nuestra hipótesis de trabajo, es que LSm1-7, Dhh1 y Pat1 son utilizadas no solo por BMV para replicar en levaduras, sino también por otros virus ARN(+) que infectan a humanos, como el virus de la hepatitis C, para replicar en células de mamíferos. Por otra parte, dado el papel clave de estas proteínas en un paso común en todos los virus de ARN(+), es esencial caracterizar los mecanismos moleculares aun no conocidos y asociados a dicha función. En este sentido, también estudiamos la hipótesis de que el complejo LSm1-7, como miembro de la familia de proteínas Sm, pueda interactuar directamente con los genomas virales de virus de ARN(+) con el fin de desempeñar su papel en el ciclo de vida del virus de una manera similar a la que otros miembros de su familia interactúan con sus ARN con el fin de lograr sus diferentes funciones celulares. En este trabajo hemos podido confirmar ambas hipótesis demostrando que los homólogos humanos de las proteínas anteriormente mencionadas, LSm1-7, Rck/p54 y PatL1, son necesarios para la traducción y replicación del ARN del virus de la Hepatitis C. Por otra parte, los anillos reconstituidos de LSm1-7 reconocen específicamente señales importantes, tanto en el genoma de BMV como en el de la Hepatitis C que regulan su traducción y/o replicación. Estas observaciones constituyen la primera evidencia de que el complejo LSm1-7 es capaz de interactuar directamente con genomas virales y representan también novedosos patrones de interacción de este complejo con ARN. Teniendo en cuenta las estrategias de replicación en común de los virus de ARN de cadena positiva y las funciones celulares conservadas de LSm1-7, Pat1 y Dhh1 de levaduras a humanos, nuestros resultados señalan la posibilidad de explotar estas proteínas para la generación de medicamentos antivirales de amplio espectro.
Hopkins, M. J. "Characterisation of the human intestinal microbiota based on community cellular fatty acid analysis and ribosomal RNA measurements". Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604222.
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