Bibliografías temáticas / Cats as laboratory animals

Índice

  1. Artículos de revistas
  2. Tesis
  3. Libros
  4. Capítulos de libros
  5. Actas de conferencias
  6. Informes

Literatura académica sobre el tema "Cats as laboratory animals"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 24 de febrero de 2023

Crea una cita precisa en los estilos APA, MLA, Chicago, Harvard y otros

Elija tipo de fuente:

Consulte las listas temáticas de artículos, libros, tesis, actas de conferencias y otras fuentes académicas sobre el tema "Cats as laboratory animals".

Junto a cada fuente en la lista de referencias hay un botón "Agregar a la bibliografía". Pulsa este botón, y generaremos automáticamente la referencia bibliográfica para la obra elegida en el estilo de cita que necesites: APA, MLA, Harvard, Vancouver, Chicago, etc.

También puede descargar el texto completo de la publicación académica en formato pdf y leer en línea su resumen siempre que esté disponible en los metadatos.

Artículos de revistas sobre el tema "Cats as laboratory animals"

1

Lobova, D., V. Kleinova, J. Konvalinova, P. Cerna y D. Molinkova. "Laboratory diagnostics of selected feline respiratory pathogens and their prevalence in the Czech Republic". Veterinární Medicína 64, No. 1 (23 de enero de 2019): 25–32. http://dx.doi.org/10.17221/93/2017-vetmed.

Texto completo
Resumen
Respiratory problems in cats have a multifactorial character. Therapy without the detection of pathogen is often ineffective. Our study was therefore focused on the detection of important feline respiratory bacterial pathogens such as Mycoplasma felis, Chlamydia felis and Bordetella bronchiseptica and viral pathogens such as Felid alphaherpesvirus-1 and feline calicivirus. The goal of this study was to map the occurrence of these pathogens in cat populations in the Czech Republic with the aim of introducing rapid and highly sensitive methods into routine diagnostics and to provide consulting services to animal health professionals based on the acquired data. A total of 218 cats were investigated in the study: 69 were outdoor and 149 were indoor cats. Three groups of animals were compared: up to one year of age (60 cats), one to three years of age (68 cats) and more than three years of age (90 cats). Samples were taken from conjunctiva and/or the oropharynx. Samples originated from cats with various forms of respiratory disease or from healthy cats from different parts of the Czech Republic. Real-Time RT-PCR, multiplex Real-Time PCR, nested PCR and sequencing analyses were performed. Outdoor cats were infected more often (84 detected pathogens in 69 cats) than indoor cats (110 detected infections in 149 cats). More than one pathogen was detected in a total of 38 cats, and six cats were infected with more than two pathogens. The difference was statistically significant in the case of co-infections, but not for mono-infections (P < 0.05). Kittens and young adults up to the age of one year were the most common reservoirs of respiratory infections (only 19 cats out of 60 were negative and positive cats often harboured coinfections). The difference in age groups were not statistically significant (P > 0.05). Concerning the site of the sampling, feline calicivirus, M. felis and B. bronchiseptica were detected more often from oropharynx than from conjunctival swabs. M. felis was slightly more common in clinically diseased animals (39.6%) than in healthy ones (26.1%). The obtained results reveal the frequency of individual pathogens and their co-infections in cats kept on the territory of the Czech Republic, data which can be used to make the treatment of respiratory infections and breeding measures more effective. Therefore, the diagnostic methods are now available to veterinary surgeons with the possibility of consultation and discussion of the results.
Los estilos APA, Harvard, Vancouver, ISO, etc.
2

de Rivera, Christina, Jacqui Ley, Bill Milgram y Gary Landsberg. "Development of a laboratory model to assess fear and anxiety in cats". Journal of Feline Medicine and Surgery 19, n.º 6 (18 de abril de 2016): 586–93. http://dx.doi.org/10.1177/1098612x16643121.

Texto completo
Resumen
Objectives The objectives of this study were: (1) to develop a laboratory-based model to assess fear and anxiety in cats using the feline open-field test (OFT) and the feline human interaction test (HIT); and (2) to validate the model using diazepam, a known anxiolytic. Methods Laboratory-housed cats (n = 41) were first classified as fearful, mildly fearful or non-fearful by a technician familiar with the cats and also by veterinary behaviorists (GL, JL), by assessing the cats’ behavior in their home rooms. In experiment 1, each cat’s behavior was assessed in an OFT and an HIT. In experiment 2, after administration of the anxiolytic diazepam, a subset of the cats was re-tested. Results In experiment 1, the OFT revealed significant group effects on two measures: duration of inactivity, and vocalization. Fearful animals had significantly longer periods of inactivity than non-fearful animals. Non-fearful and mildly fearful cats vocalized more frequently than fearful cats. In the HIT, fearful cats travelled less than non-fearful and mildly fearful cats. Fearful and mildly fearful animals had significantly longer durations of inactivity, and non-fearful and mildly fearful cats had a significantly higher frequency of vocalization compared with fearful cats. In experiment 2, in the OFT, treatment with diazepam caused an increase in distance travelled, shorter durations of inactivity, and more frequent inactivity and vocalization. In the HIT, diazepam increased distance travelled and decreased duration of inactivity. Fearful cats spent significantly less time near the human compared with non-fearful cats, and this persisted under diazepam. Conclusions and relevance The feline OFT and feline HIT can be used jointly to assess the effects of medications or other therapies on fear and anxiety in the domestic cat.
Los estilos APA, Harvard, Vancouver, ISO, etc.
3

Fischer, Amy, Valerie AW Benka, Joyce R. Briggs, Joanne Maki, Kevin N. Morris, Kayla A. Myers, Linda Rhodes, George Robert Weedon y Julie K. Levy. "Hybrid model intermediate between a laboratory and field study: A humane paradigm shift in feline research". Journal of Feline Medicine and Surgery 20, n.º 9 (19 de julio de 2018): 803–10. http://dx.doi.org/10.1177/1098612x18791872.

Texto completo
Resumen
Objectives: Non-surgical contraceptives are under development to provide accessible, affordable and humane alternatives for the management of free-roaming cat populations. The objective of this project was to develop a research approach for promising non-surgical contraceptives using outbred cats in a simulated free-roaming setting, meeting high standards for both animal welfare and scientific rigor. Methods: A facility, specially constructed with indoor and outdoor living areas, was approved and regulated as both an animal shelter and a United States Department of Agriculture research facility. Thirty female and five male cats, healthy but at high risk of euthanasia, were recruited from animal shelters and private homes. Guided by a detailed protocol, cats were housed in this facility for up to 18 months after acclimatization. Cats were administered the study product or a placebo, and then entered into a breeding trial. Cats were adopted at the end of the study. A range of methods was used to provide enrichment and balance a natural environment with the need for detailed daily monitoring. Results: Primary study results related to contraceptive safety and efficacy are published separately. Achieving a research model that is an intermediate step between a laboratory and an uncontained free-roaming cat colony was complex. Significant learnings shared in this current publication span: the selection of cats; acclimatization to a simulated colony environment; cat behavioral training during the study and in preparation for adoption; disease management; contract staff and volunteer support; and cat behavior throughout a breeding study. Conclusions and relevance: This model inspires continued movement away from the paradigm of breeding cats for research and instead sources existing cats at risk for euthanasia. The housing and management of the cats elevates research animals’ quality of life and provides positive post-study outcomes. While not appropriate for every feline research scenario, this hybrid model (between a laboratory and field study) proved to be a practical, humane and reliable scenario for research requiring a simulated real-world environment.
Los estilos APA, Harvard, Vancouver, ISO, etc.
4

Ujwala Ashokrao Jadhao, Chatraguna Eknathrao Lagad y Rajesh Kundlikrao Ingole. "Laboratory animal handling techniques, basic facilities and care: A review". World Journal of Biology Pharmacy and Health Sciences 12, n.º 3 (30 de diciembre de 2022): 170–82. http://dx.doi.org/10.30574/wjbphs.2022.12.3.0239.

Texto completo
Resumen
Animals have been utilized in pharmaceutical scientific research and development for more than 300 years. Pharmaceutical items are a must in order to treat illnesses caused by viruses, bacteria, or homeostatic imbalance, among other causes. Pharmaceuticals must be thoroughly characterized via in vitro, in vivo, and clinical trial investigations in order to be used in humans. It is essential to quantify organism's in vivo using animals whose physiology and genetics are most similar to those of humans. Common animals used for laboratory experimental research are Cats, Rats, Dogs, Mice, Guinea pigs and Monkeys. Handling of animals includes transportation, animal housing, temperature of cages, sanitation, water, and food options etc. Proper animal handling techniques affects the outcome of research study, so it very imperative to study and to practice the basics of animal handling techniques. This review is the small efforts to compile the scientific literature and basic information regarding laboratory animal handling techniques, basic facility and care.
Los estilos APA, Harvard, Vancouver, ISO, etc.
5

Ruthrauff, Cassandra M., Julie Smith y Leigh Glerum. "Primary Bacterial Septic Peritonitis in Cats: 13 Cases". Journal of the American Animal Hospital Association 45, n.º 6 (1 de noviembre de 2009): 268–76. http://dx.doi.org/10.5326/0450268.

Texto completo
Resumen
The purpose of this paper is to describe the signalment, clinical signs, laboratory results, culture results, and response to treatment for primary septic peritonitis in cats. This is a retrospective study of 12 client-owned animals. Medical records were reviewed for clinical findings, laboratory results, microbial culture results, radiographic findings, diagnosis, treatment, and outcome. The overall mortality rate for this group of cats was 31%, consistent with previous reports of septic peritonitis in cats. All cats that were both bradycardic and hypothermic on presentation did not survive. Other clinicopathological findings were consistent with previously reported cases of septic peritonitis in cats. Results suggest that clinicopathological findings and outcomes in cats with primary septic peritonitis are similar to those in cats with septic peritonitis from a determined cause. A specific mechanism of inoculation has yet to be determined, but an oral source of bacteria is suggested for cats with primary bacterial septic peritonitis.
Los estilos APA, Harvard, Vancouver, ISO, etc.
6

Cossaboom, Caitlin M., Alexandra M. Medley, Jessica R. Spengler, Esther A. Kukielka, Grace W. Goryoka, Tiffany Baird, Swity Bhavsar et al. "Low SARS-CoV-2 Seroprevalence and No Active Infections among Dogs and Cats in Animal Shelters with Laboratory-Confirmed COVID-19 Human Cases among Employees". Biology 10, n.º 9 (11 de septiembre de 2021): 898. http://dx.doi.org/10.3390/biology10090898.

Texto completo
Resumen
Human-to-animal and animal-to-animal transmission of SARS-CoV-2 has been documented; however, investigations into SARS-CoV-2 transmission in congregate animal settings are lacking. We investigated four animal shelters in the United States that had identified animals with exposure to shelter employees with laboratory-confirmed COVID-19. Of the 96 cats and dogs with specimens collected, only one dog had detectable SARS-CoV-2 neutralizing antibodies; no animal specimens had detectable viral RNA. These data indicate a low probability of human-to-animal transmission events in cats and dogs in shelter settings with early implementation of infection prevention interventions.
Los estilos APA, Harvard, Vancouver, ISO, etc.
7

Calvet, Guilherme Amaral, Sandro Antonio Pereira, Maria Ogrzewalska, Alex Pauvolid-Corrêa, Paola Cristina Resende, Wagner de Souza Tassinari, Anielle de Pina Costa et al. "Investigation of SARS-CoV-2 infection in dogs and cats of humans diagnosed with COVID-19 in Rio de Janeiro, Brazil". PLOS ONE 16, n.º 4 (28 de abril de 2021): e0250853. http://dx.doi.org/10.1371/journal.pone.0250853.

Texto completo
Resumen
Background Infection by SARS-CoV-2 in domestic animals has been related to close contact with humans diagnosed with COVID-19. Objectives: To assess the exposure, infection, and persistence by SARS-CoV-2 of dogs and cats living in the same households of humans that tested positive for SARS-CoV-2, and to investigate clinical and laboratory alterations associated with animal infection. Methods Animals living with COVID-19 patients were longitudinally followed and had nasopharyngeal/oropharyngeal and rectal swabs collected and tested for SARS-CoV-2. Additionally, blood samples were collected for laboratory analysis, and plaque reduction neutralization test (PRNT90) to investigate specific SARS-CoV-2 antibodies. Results Between May and October 2020, 39 pets (29 dogs and 10 cats) of 21 patients were investigated. Nine dogs (31%) and four cats (40%) from 10 (47.6%) households were infected with or seropositive for SARS-CoV-2. Animals tested positive from 11 to 51 days after the human index COVID-19 case onset of symptoms. Three dogs tested positive twice within 14, 30, and 31 days apart. SARS-CoV-2 neutralizing antibodies were detected in one dog (3.4%) and two cats (20%). In this study, six out of thirteen animals either infected with or seropositive for SARS-CoV-2 have developed mild but reversible signs of the disease. Using logistic regression analysis, neutering, and sharing bed with the ill owner were associated with pet infection. Conclusions The presence and persistence of SARS-CoV-2 infection have been identified in dogs and cats from households with human COVID-19 cases in Rio de Janeiro, Brazil. People with COVID-19 should avoid close contact with their pets during the time of their illness.
Los estilos APA, Harvard, Vancouver, ISO, etc.
8

Grotheer, Maike, Johannes Hirschberger, Katrin Hartmann, Noemi Castelletti y Bianka Schulz. "Comparison of signalment, clinical, laboratory and radiographic parameters in cats with feline asthma and chronic bronchitis". Journal of Feline Medicine and Surgery 22, n.º 7 (4 de septiembre de 2019): 649–55. http://dx.doi.org/10.1177/1098612x19872428.

Texto completo
Resumen
Objectives Feline asthma (FA) and feline chronic bronchitis (CB) are common respiratory conditions in cats, frequently referred to as ‘feline lower airway disease’. However, the aetiologies of both inflammatory airway diseases are probably different. Little is known about the differences in signalment, clinical signs, laboratory abnormalities and radiographic features between cats with these two airway diseases. The aim of the study was to investigate whether certain parameters can help in differentiating between both diseases, as distinguished by airway cytology. Methods Seventy-three cats with FA and 24 cats with CB were included in the retrospective study. Inclusion criteria were compatible clinical signs and a cytological evaluation of bronchoalveolar lavage fluid indicating either FA (eosinophilic inflammation) or CB (neutrophilic inflammation) without cytological or microbiological evidence of bacterial infection. Parameters of signalment, physical examination, haematology and thoracic radiographs of both disease groups were compared statistically ( P <0.05). Results The median age of cats with FA was 6 years, and was 7.5 years in cats with CB ( P = 0.640). The most commonly reported clinical signs in both groups were a cough (95% FA/96% CB; P = 1.000), pathological pulmonary auscultatory sounds (82% FA/79% CB; P = 0.766) and dyspnoea (73% FA/79% CB; P = 0.601). Abnormal radiographic lung patterns were detected in 94% of cats with FA and 91% with CB ( P = 0.629), respectively. Blood eosinophilia was significantly more common in cats with FA (40%) compared with CB (27%) ( P = 0.026). Conclusions and relevance The study indicates that a differentiation of FA and CB by means of signalment, a single clinical sign, and haematological and radiographic findings is not possible.
Los estilos APA, Harvard, Vancouver, ISO, etc.
9

Carlstead, Kathy, Janine L. Brown y William Strawn. "Behavioral and physiological correlates of stress in laboratory cats". Applied Animal Behaviour Science 38, n.º 2 (noviembre de 1993): 143–58. http://dx.doi.org/10.1016/0168-1591(93)90062-t.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
10

Platto, Sara, Agathe Serres y Ai Jingyi. "Chinese College Students’ Attitudes towards Animal Welfare". Animals 12, n.º 2 (10 de enero de 2022): 156. http://dx.doi.org/10.3390/ani12020156.

Texto completo
Resumen
Understanding the attitude of stakeholders towards animals is critical for the development and improvement of animal welfare in a country. College students from veterinary, animal, and life sciences majors represent future key stakeholders that will interact with professionals from animal industries. Therefore, it is critical to understand these college students’ attitudes towards animals and their knowledge about animal welfare. The present survey aimed to investigate Chinese college students’ concerns towards different animal classes (i.e., pets, farm, laboratory, and wild animals) through the animal Sentient and Five Freedoms models. Chinese college students from different majors (i.e., related to animal sciences or not) scored very well in their attitude towards both the animal Sentient and Five Freedoms models, with differences depending on the animal class considered. Pets (dogs and cats) had better consideration for both animal Sentient and Five Freedoms models, followed by wild animals, while farm and laboratory animals were less considered. Veterinary science major students showed the strongest differences in attitudes depending on the animal classes considered compared to other majors. Furthermore, respondents showed better attitude scoring if they currently owned or had owned animals, had participated in animal welfare courses, or in laboratory work that involved animals. When compared to previous studies, our results suggest a general improvement of Chinese college students’ attitudes towards animals.
Los estilos APA, Harvard, Vancouver, ISO, etc.
Más fuentes

Tesis sobre el tema "Cats as laboratory animals"

1

Crawley, Allison Catherine. "Enzyme replacement therapy in a feline model of mucopolysaccharidosis type VI /". Title page, contents and abstract only, 1998. http://web4.library.adelaide.edu.au/theses/09PH/09phc9107.pdf.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
2

Yogalingam, Gouri. "Molecular characterisation of feline MPS VI and evaluation of gene therapy /". Title page, contents and abstract only, 1998. http://web4.library.adelaide.edu.au/theses/09PH/09phy54.pdf.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
3

Zarrinkalam, Krystyna. "Characterisation of osteoblast function in a feline model of mucopolysaccharidosis type VI". Title page, contents and introduction only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phz38.pdf.

Texto completo
Resumen
Addenda slip inserted in back. Includes bibliographical references (leaves 178-231). To further the understanding of the molecular mechanisms that contribute to the skeletal pathology of mucopolysaccharidosis type VI and to investigate the production of organic matrix by mucopolysaccharidosis VI osteoblasts
Los estilos APA, Harvard, Vancouver, ISO, etc.
4

Rose, Heidi Huffman. "SURVIVIN AND p53 EXPRESSION IN FELINE ORAL SQUAMOUS CELL CARCINOMA AND CORRELATION WITH PROGNOSIS". MSSTATE, 2008. http://sun.library.msstate.edu/ETD-db/theses/available/etd-03252008-191425/.

Texto completo
Resumen
Squamous cell carcinoma (SCC), the most common oral neoplasm of cats, demonstrates aggressive local invasion and has a poor prognosis. In humans, mutation of the p53 gene, crucial in cell cycle arrest and induction of apoptosis in damaged cells, is common in neoplasms. Survivin, an inhibitor of apoptosis, is frequently overexpressed in many types of human cancer. Studies suggest that wild-type p53 inhibits survivin expression, while mutated p53 does not. The purposes of this study included immunohistochemical examination of survivin and p53 expression in feline oral SCC and determination of a correlation between p53 mutation and survivin overexpression, as well as comparison with survival time. Survivin expression was noted in 80% (24/30) of cases, while 43.3% (13/30) of cases were positive for p53. No statistically significant correlation was noted between p53 and survivin expression, even when corrected for age, breed, and sex; and survival time was not affected.
Los estilos APA, Harvard, Vancouver, ISO, etc.
5

Rochlitz, Irene. "The welfare of cats kept in confined environments". Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271003.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
6

Kirk, Robert George William. "Reliable animals, responsible scientists : constructing standard laboratory animals in Britain c.1919-1976". Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1445731/.

Texto completo
Resumen
This thesis explores the attempt to construct a national supply of standard laboratory- animals in Britain between 1919 and 1976. The demand for a national supply of standard laboratory-animals is located in the formation during the interwar period of the discipline of biological standardization. In contrast to other disciplines within the sciences biological standardization placed great emphasis upon the routine replication of experimental results. In consequence the field of biological standardization problematized the laboratory-animal and sought its standardization in order to construct it as a reliable diagnostic tool. In 1947 the Medical Research Council responded to pressure from an unprecedented coalition of scientific societies and established the Laboratory Animals Bureau tasked with regulating the British laboratory-animal production toward producing standard laboratory-animals. The work of the Laboratory Animal Bureau is analysed but the main focus of the thesis is upon the relationship between the practices of standardization and the promotion of the welfare of laboratory-animals. Particularly after the close of the Second World War the project to standardize laboratory-animals became increasingly associated with the promotion of their welfare. The relationship between the two was made explicit through the work of the Universities Federation for Animal Welfare in collaboration with the Laboratory Animals Bureau. In order to understand the relationship between standardization and welfare Michel Foucault's concept of biopower is employed. It is subsequently argued that the analytics of biopower need not be restricted to human life but equally encompasses non-human life. Through the Foucaultian perspective of biopower it is argued that standardization and welfare are two poles of the same biopolitical process.
Los estilos APA, Harvard, Vancouver, ISO, etc.
7

Lazenby, Billie Theresa. "Do feral cats affect small animals? : a case study from the forests of southern Tasmania". Phd thesis, School of Biological Sciences, 2012. http://hdl.handle.net/2123/9990.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
8

Shapiro, Amanda June. "Australian companion animals and Coxiella burnetii (Q fever)". Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/15780.

Texto completo
Resumen
Introduction: Outbreaks of Q fever, an emerging worldwide zoonosis caused by Coxiella burnetii, were associated with periparturient companion animals in veterinary hospitals in Australia. These outbreaks initiated feline and canine seroprevalence studies, a national cat breeder survey, a molecular study looking for C. burnetii within food sources, and a canine vector-borne diseases study in dogs from Aboriginal communities, with the aim of increasing our understanding of the epidemiology of C. burnetii infection in companion animals. Method: Seroprevalence studies evaluated three methodologies; indirect immunofluorescence assay, enzyme linked immunosorbent assay and complement fixation testing. The Q fever cat breeder survey recruited registered cat breeders to determine their knowledge, attitudes and practices. The molecular study utilised real-time PCR to screen raw meat (n=59) intended for pet consumption containing kangaroo (n=55) and non-kangaroo tissues (n=4). Results: Seroprevalence was highest in cattery-confined breeding cats (9.3%) and in dogs from Aboriginal communities (6.5%). A significant Q fever knowledge gap was present amongst cat breeders, with potential associations between risky husbandry practices and Q fever transmission. C. burnetii DNA was detected in 64% of samples, all of kangaroo origin, with sequence confirmation and genotyping of three Australian strains isolated from human Q fever patients. Anaemia and thrombocytopenia the most common haematological abnormalities in dogs from Aboriginal communities were likely attributable to Anaplasma platys and Babesia vogeli infections, but also affected by nutritional, parasitic and other environmental stressors. Conclusion: Australian cats and dogs have been exposed to C. burnetii; reproducing infected animals pose a potential public health threat to those present at parturition. A comprehensive risk assessment of novel sources of C. burnetii infection for companion animals and humans is required.
Los estilos APA, Harvard, Vancouver, ISO, etc.
9

Agarwal, Rajat. "A model for minimizing cost for housing laboratory mice". [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0001241.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
10

Delpire, Veronique Charline. "Ethical schemes for the use of transgenic laboratory animals". Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324118.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
Más fuentes

Libros sobre el tema "Cats as laboratory animals"

1

A, Suckow Mark, ed. The laboratory cat. Boca Raton, Fla: CRC Press, 1998.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
2

Scientific and humane issues in the use of random source dogs and cats in research. Washington, D.C: National Academies Press, 2009.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
3

Donnersberger, Anne B. A manual of anatomy and physiology: Laboratory animal, the cat. 3a ed. Lexington, Mass: D.C. Heath, 1985.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
4

Willard, Michael D. y Harold Tvedten. Small animal clinical diagnosis by laboratory methods. 5a ed. St. Louis, Mo: Elsevier, 2012.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
5

Sodikoff, Charles. Laboratory profiles of small animal diseases: A guide to laboratory diagnosis. 2a ed. St. Louis: Mosby, 1995.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
6

Laboratory profiles of small animal diseases: A guide to laboratory diagnosis. 3a ed. St. Louis: Mosby, 2001.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
7

Bush, B. M. Interpretation of laboratory results for small animal clinicians. Oxford: Blackwell Scientific Publications, 1991.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
8

D, Willard Michael, Tvedten Harold y Turnwald Grant H, eds. Small animal clinical diagnosis by laboratory methods. 2a ed. Philadelphia: W.B. Saunders, 1994.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
9

Kustritz, Margaret V. Root. Clinical canine and feline reproduction: Evidence-based answers. Ames, Iowa: Wiley-Blackwell, 2010.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
10

Amemiya, T. Retinal and choroidal vascular changes and systemic diseases in rats: Corrosion cast and scanning electron microscopy. New York: Springer, 2003.

Buscar texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
Más fuentes

Capítulos de libros sobre el tema "Cats as laboratory animals"

1

Gaskell, R. M. "Herpesvirus of Cats". En Virus Diseases in Laboratory and Captive Animals, 219–32. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-2091-3_13.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
2

Blenden, Donald C., Manuel J. Torres-Anjel y F. T. Satalowich. "Applications of Laboratory Technology in the Evaluation of the Risk of Rabies Transmissions by Biting Dogs and Cats". En Advances in Animal Welfare Science 1985, 221–46. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4247-9_12.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
3

Lakshmanan, Mageshwaran. "Laboratory Animals". En Introduction to Basics of Pharmacology and Toxicology, 13–36. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5343-9_2.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
4

Gunn-Moore, Danièlle y Kerry Simpson. "Pheochromocytoma in Cats". En Clinical Endocrinology of Companion Animals, 137–42. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch14.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
5

Gunn-Moore, Danièlle y Kerry Simpson. "Hypoadrenocorticism in Cats". En Clinical Endocrinology of Companion Animals, 22–27. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch2.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
6

Gunn-Moore, Danièlle y Kerry Simpson. "Insulinoma in Cats". En Clinical Endocrinology of Companion Animals, 240–44. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch22.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
7

Gunn-Moore, Danièlle. "Hypothyroidism in Cats". En Clinical Endocrinology of Companion Animals, 273–77. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch26.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
8

Peterson, Mark E. "Hyperthyroidism in Cats". En Clinical Endocrinology of Companion Animals, 295–310. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch29.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
9

Schenck, Patricia A. y Dennis Chew. "Hypocalcemia in Cats". En Clinical Endocrinology of Companion Animals, 326–34. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch32.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
10

Chew, Dennis y Patricia A. Schenck. "Hypercalcemia in Cats". En Clinical Endocrinology of Companion Animals, 373–84. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118997093.ch35.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.

Actas de conferencias sobre el tema "Cats as laboratory animals"

1

Kulmakova, N. I. "PREVALENCE AND FEATURE OF OTODEKTOSIS IN CATS". En DIGEST OF ARTICLES ALL-RUSSIAN (NATIONAL) SCIENTIFIC AND PRACTICAL CONFERENCE "CURRENT ISSUES OF VETERINARY MEDICINE: EDUCATION, SCIENCE, PRACTICE", DEDICATED TO THE 190TH ANNIVERSARY FROM THE BIRTH OF A.P. Stepanova. Publishing house of RGAU - MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1853-9-2021-45.

Texto completo
Resumen
The prevalence and peculiarities of the course of otodectosis in cats in the conditions of the Vysota veterinary clinic (Moscow) have been studied. Animals of both sexes are at risk for the incidence of otodectosis; outbred and belonging to breeds (for example, Scottish); crossbred cats in a mixed way of keeping; cats under 6 years old; animals that have a lot of contacts with other animals. Otodectosis in the population of the city of Moscow is especially pronounced in the autumn-spring period.
Los estilos APA, Harvard, Vancouver, ISO, etc.
2

Veretnikov, A. A., O. N. Konyaeva y V. A. Tolkachѐv. "Age-related incidence of calculus diagnosis in purebreds dogs and cats at home". En SCIENCE OF RUSSIA: GOALS AND OBJECTIVES. L-Journal, 2020. http://dx.doi.org/10.18411/sr-10-12-2020-42.

Texto completo
Resumen
A study of the age-related incidence of calculus diagnosis in certain breeds of dogs and cats was carried out. Because of the research, it was found that at all ages taken into account; dogs are most susceptible to animals of the Chihuahua Hua, Cocker Spaniel and Spitz breeds, and in cats - animals of the Scottish, British and Siamese breeds.
Los estilos APA, Harvard, Vancouver, ISO, etc.
3

Monteiro, B., C. Otis, L. Arendt-Nielsen y E. Troncy. "Quantitative Sensory Testing in Dogs and Cats with Osteoarthritis-Related Pain: A Systematic Review". En Pain in Animals Workshop 2017: Abstracts. Schattauer GmbH, 2018. http://dx.doi.org/10.1055/s-0038-1660885.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
4

Koniar, Dusan, Libor Hargas, Zuzana Loncova, Frantisek Duchon y Peter Beno. "Laboratory animals tracking in videosequences". En 2016 ELEKTRO. IEEE, 2016. http://dx.doi.org/10.1109/elektro.2016.7512134.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
5

Naida, Christopher G. "Construction Vibration Mitigation for Laboratory Animals". En Geotechnical Earthquake Engineering and Soil Dynamics V. Reston, VA: American Society of Civil Engineers, 2018. http://dx.doi.org/10.1061/9780784481462.030.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
6

Krasnikov, A. V. "SOME ASPECTS OF MAXILLOFACIAL SURGERY OF SMALL NONPRODUCTIVE ANIMALS". En DIGEST OF ARTICLES ALL-RUSSIAN (NATIONAL) SCIENTIFIC AND PRACTICAL CONFERENCE "CURRENT ISSUES OF VETERINARY MEDICINE: EDUCATION, SCIENCE, PRACTICE", DEDICATED TO THE 190TH ANNIVERSARY FROM THE BIRTH OF A.P. Stepanova. Publishing house of RGAU - MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1853-9-2021-42.

Texto completo
Resumen
An analytical review is presented, the purpose of which is todetermine the most important vectors when choosing approaches to reparative osteogenesis in dogs and cats with fractures of the bones of the upper and / or lower jaw. Medical care for all types of fractures should rely on such vectors of functionally stable osteosynthesis as adequate rigid fixation, minimally invasiveness, structural stability, safety and effectiveness of the new product's effect on bone regeneration and on the whole organism as a whole.
Los estilos APA, Harvard, Vancouver, ISO, etc.
7

Lukanc, Barbara y Vladimira Erjavec. "Treating Burns in Cats and Dogs Using Medical Honey". En Socratic Lectures 7. University of Lubljana Press, 2022. http://dx.doi.org/10.55295/psl.2022.d11.

Texto completo
Resumen
Common causes of burns in small animals are household accidents or iatrogenic burns in veterinary clinics (electric heating pads…). Burns are usually treated as open wounds, where honey can be used as a dressing, that creates a moist healing environment, promotes tissue debridement, eliminates infections, has a deodorizing effect, and reduces inflammation, edema, and exudation. Honey also stimulates angiogenesis, promotes granulation tissue and epithelialization, and reduces scarring. It is important to minimise the potential contamination of burns. The high viscosity of honey acts as a physical barrier against external contaminants and the effectiveness of the barrier is enhanced by the antibacterial properties of honey. In general, antibiotics are not necessary but they are indicated in septic animals. In early stages of burn healing excessive exudate occurs, requiring dressings to be changed up to twice daily. In later stages, they are usually changed every 2-3 days. For uneventful healing, the primary layer should not adhere to the wound, which is not achieved by the application of honey. Therefore, after the application of honey we covered wounds with low-adherent absorbent dressing. We describe the treatment of a chemical burn from an iodine dressing in a cat, a thermal burn from spilled boiling soup in a cat, and a thermal burn from a heating pad in a dog with medical honey. All wounds were treated as open wounds and healed completely by second intention healing. Keywords: Burns; Cats; Dogs; Medical honey dressing
Los estilos APA, Harvard, Vancouver, ISO, etc.
8

Noble, C., E. Scott, A. Nolan y J. Reid. "Initial Evidence to Support the Use of a Generic Health-Related Quality of Life Instrument to Measure Chronic Pain in Cats with Osteoarthritis". En Pain in Animals Workshop 2017: Abstracts. Schattauer GmbH, 2018. http://dx.doi.org/10.1055/s-0038-1660887.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
9

Dyulger, G. P. "UTERINE STROKE PIOMETER IN A DOG ASSOCIATED WITH OVARIOREMINANT SYNDROME (CLINICAL CASE)". En DIGEST OF ARTICLES ALL-RUSSIAN (NATIONAL) SCIENTIFIC AND PRACTICAL CONFERENCE "CURRENT ISSUES OF VETERINARY MEDICINE: EDUCATION, SCIENCE, PRACTICE", DEDICATED TO THE 190TH ANNIVERSARY FROM THE BIRTH OF A.P. Stepanova. Publishing house of RGAU - MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1853-9-2021-35.

Texto completo
Resumen
Pyometra of the uterine stump associated with the ovarian remnant syndrome is quite rare found in veterinary practice of small domestic animals (dogs and cats) and therefore the description of its particular case in a dog is undoubtedly of some interest to practicing doctors.
Los estilos APA, Harvard, Vancouver, ISO, etc.
10

Maxwell, Paul, Howard Jay Siegel y Jerry Potter. "The ISTeC People-Animals-Robots laboratory: Robust resource allocation". En Rescue Robotics (SSRR). IEEE, 2009. http://dx.doi.org/10.1109/ssrr.2009.5424142.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.

Informes sobre el tema "Cats as laboratory animals"

1

Zurlo, Joanne. Institute of Laboratory Animals Research (ILAR). Fort Belvoir, VA: Defense Technical Information Center, marzo de 2003. http://dx.doi.org/10.21236/ada416653.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
2

Shantyz, A. K., P. V. Miroshnichenko, E. S. Sadikova y V. V. Menshenin. Changes in hematological and biochemical blood parameters in laboratory animals with experimental escherichiosis. Краснодарский научный центр по зоотехнии и ветеринарии, 2018. http://dx.doi.org/10.18411/88sh-e5337.

Texto completo
Los estilos APA, Harvard, Vancouver, ISO, etc.
3

Corscadden, Louise y Arpaporn Sutipatanasomboon. What Is Operant Behavior And How To Study It. Maze Engineers, diciembre de 2022. http://dx.doi.org/10.55157/me2022127.

Texto completo
Resumen
Operant behavior describes a type of voluntary goal-directed actions in animals based on the repercussions of previous occurrences. It develops when animals learn to specifically respond to recurring situations based on the outcome of their past experience. American psychologist B.F. Skinner was the first to use operant to describe the behaviors he observed in his landmark experiments in laboratory animals. Operant behavior and conditioning refine the nuance between conscious and unconscious behavioral responses, which influence psychology, and applied behavior analysis, and improve our understanding of addiction, substance dependence, child development, and decision-making.
Los estilos APA, Harvard, Vancouver, ISO, etc.
4

Corscadden, Louise y Arpaporn Sutipatanasomboon. Rodent Tagging And Identification. ConductScience, enero de 2023. http://dx.doi.org/10.55157/cs20230109.

Texto completo
Resumen
Animal tagging is a means to identify and distinguish all the individual animals of interest, which applies to wildlife, farm, or laboratory animals. It involves attaching a tag to a specific animal part that contains a unique identifier for each animal. The identifier can be numbers, alphabets, or a combination of both that distinguish and track the animals throughout their lifespans. In rodents, tagging is the most popular identification approach. Typically, tags are made from metals and attached to the outer part of rodent ears, or the ear pinna. In rare circumstances, metal tags can also be attached to the rodent’s leg or tail.
Los estilos APA, Harvard, Vancouver, ISO, etc.
5

Matthew, Gray. Data from "Winter is Coming – Temperature Affects Immune Defenses and Susceptibility to Batrachochytrium salamandrivorans". University of Tennessee, Knoxville Libraries, enero de 2021. http://dx.doi.org/10.7290/t7sallfxxe.

Texto completo
Resumen
Environmental temperature is a key factor driving various biological processes, including immune defenses and host-pathogen interactions. Here, we evaluated the effects of environmental temperature on the pathogenicity of the emerging fungus, Batrachochytrium salamandrivorans (Bsal), using controlled laboratory experiments, and measured components of host immune defense to identify regulating mechanisms. We found that adult and juvenile Notophthalmus viridescens died faster due to Bsal chytridiomycosis at 14 ºC than at 6 and 22 ºC. Pathogen replication rates, total available proteins on the skin, and microbiome composition likely drove these relationships. Temperature-dependent skin microbiome composition in our laboratory experiments matched seasonal trends in wild N. viridescens, adding validity to these results. We also found that hydrophobic peptide production after two months post-exposure to Bsal was reduced in infected animals compared to controls, perhaps due to peptide release earlier in infection or impaired granular gland function in diseased animals. Using our temperature-dependent infection results, we performed a geographic analysis that suggested that N. viridescens populations in the northeastern United States and southeastern Canada are at greatest risk for Bsal invasion. Our results indicate that environmental temperature will play a key role in the epidemiology of Bsal and provide evidence that temperature manipulations may be a viable Bsal management strategy.
Los estilos APA, Harvard, Vancouver, ISO, etc.
6

Jung, Carina, Matthew Carr, Denise Lindsay, Eric Fleischman y Chandler Roesch. Microbiome perturbations during domestication of the green June beetle (Cotinis nitida). Engineer Research and Development Center (U.S.), febrero de 2022. http://dx.doi.org/10.21079/11681/43342.

Texto completo
Resumen
Animal-associated microbiomes are critical to the well-being and proper functioning of the animal host, but only limited studies have examined in-sect microbiomes across different developmental stages. These studies revealed large shifts in microbiome communities, often because of significant shifts in diet during insects’ life cycle. Establishing insect colonies as model laboratory organisms and understanding how to properly feed and care for animals with complex and dynamic life cycles requires improved data. This study examined laboratory raised green June beetles (Cotinis nitida) captured from the field upon emergence from pupae. Starting with wild-caught adults, two generations of beetles were reared in the laboratory, ending with an entirely laboratory raised generation of larvae. The study compared the microbiomes of each generation and the microbiomes of larvae to adults. This study suggests that a diet of commercial, washed fruit for adults and commercial, packaged, organic alfalfa meal for larvae resulted in depauperate gut microbiome communities. Fermentative yeasts were completely absent in the laboratory-raised adults, and major bacterial population shifts occurred from one generation to the next, coupled with high morbidity and mortality in the laboratory-raised generation. Providing laboratory-raised beetles fresh-collected fruit and the larvae field-harvested detritus may therefore vastly improve their health and survival.
Los estilos APA, Harvard, Vancouver, ISO, etc.
7

Shpigel, Nahum, Raul Barletta, Ilan Rosenshine y Marcelo Chaffer. Identification and characterization of Mycobacterium paratuberculosis virulence genes expressed in vivo by negative selection. United States Department of Agriculture, enero de 2004. http://dx.doi.org/10.32747/2004.7696510.bard.

Texto completo
Resumen
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of a severe inflammatory bowel disease (IBD) in ruminants, known as Johne’s disease or paratuberculosis. Johne’s disease is considered to be one of the most serious diseases affecting dairy cattle both in Israel and worldwide. Heavy economic losses are incurred by dairy farmers due to the severe effect of subclinical infection on milk production, fertility, lower disease resistance and early culling. Its influence in the United States alone is staggering, causing an estimated loss of $1.5 billion to the agriculture industry every year. Isolation of MAP from intestinal tissue and blood of Crohn's patients has lead to concern that it plays a potential pathogenic role in promoting human IDB including Crohn’s disease. There is great concern following the identification of the organism in animal products and shedding of the organism to the environment by subclinically infected animals. Little is known about the molecular basis for MAP virulence. The goal of the original proposed research was to identify MAP genes that are required for the critical stage of initial infection and colonization of ruminants’ intestine by MAP. We proposed to develop and use signature tag mutagenesis (STM) screen to find MAP genes that are specifically required for survival in ruminants upon experimental infection. This research projected was approved as one-year feasibility study to prove the ability of the research team to establish the animal model for mutant screening and alternative in-vitro cell systems. In Israel, neonatal goat kids were repeatedly inoculated with either one of the following organisms; MAP K-10 strain and three transposon mutants of K-10 which were produced and screened by the US PI. Six months after the commencement of inoculation we have necropsied the goats and taken multiple tissue samples from the jejunum, ileum and mesenteric lymph nodes. Both PCR and histopathology analysis indicated on efficient MAP colonization of all the inoculated animals. We have established several systems in the Israeli PI’s laboratory; these include using IS900 PCR for the identification of MAP and using HSP65-based PCR for the differentiation between MAV and MAP. We used Southern blot analysis for the differentiation among transposon mutants of K-10. In addition the Israeli PI has set up a panel of in-vitro screening systems for MAP mutants. These include assays to test adhesion, phagocytosis and survival of MAP to/within macrophages, assays that determine the rate of MAPinduced apoptosis of macrophages and MAP-induced NO production by macrophages, and assays testing the interference with T cell ã Interferon production and T cell proliferation by MAP infected macrophages (macrophage studies were done in BoMac and RAW cell lines, mouse peritoneal macrophages and bovine peripheral blood monocytes derived macrophages, respectively). All partners involved in this project feel that we are currently on track with this novel, highly challenging and ambitious research project. We have managed to establish the above described research systems that will clearly enable us to achieve the original proposed scientific objectives. We have proven ourselves as excellent collaborative groups with very high levels of complementary expertise. The Israeli groups were very fortunate to work with the US group and in a very short time period to master numerous techniques in the field of Mycobacterium research. The Israeli group has proven its ability to run this complicated animal model. This research, if continued, may elucidate new and basic aspects related to the pathogenesis MAP. In addition the work may identify new targets for vaccine and drug development. Considering the possibility that MAP might be a cause of human Crohn’s disease, better understanding of virulence mechanisms of this organism might also be of public health interest as well.
Los estilos APA, Harvard, Vancouver, ISO, etc.
8

Cahaner, Avigdor, Susan J. Lamont, E. Dan Heller y Jossi Hillel. Molecular Genetic Dissection of Complex Immunocompetence Traits in Broilers. United States Department of Agriculture, agosto de 2003. http://dx.doi.org/10.32747/2003.7586461.bard.

Texto completo
Resumen
Objectives: (1) Evaluate Immunocompetence-OTL-containing Chromosomal Regions (ICRs), marked by microsatellites or candidate genes, for magnitude of direct effect and for contribution to relationships among multiple immunocompetence, disease-resistance, and growth traits, in order to estimate epistatic and pleiotropic effects and to predict the potential breeding applications of such markers. (2) Evaluate the interaction of the ICRs with genetic backgrounds from multiple sources and of multiple levels of genetic variation, in order to predict the general applicability of molecular genetic markers across widely varied populations. Background: Diseases cause substantial economic losses to animal producers. Emerging pathogens, vaccine failures and intense management systems increase the impact of diseases on animal production. Moreover, zoonotic pathogens are a threat to human food safety when microbiological contamination of animal products occurs. Consumers are increasingly concerned about drug residues and antibiotic- resistant pathogens derived from animal products. The project used contemporary scientific technologies to investigate the genetics of chicken resistance to infectious disease. Genetic enhancement of the innate resistance of chicken populations provides a sustainable and ecologically sound approach to reduce microbial loads in agricultural populations. In turn, animals will be produced more efficiently with less need for drug treatment and will pose less of a potential food-safety hazard. Major achievements, conclusions and implications:. The PI and co-PIs had developed a refined research plan, aiming at the original but more focused objectives, that could be well-accomplished with the reduced awarded support. The successful conduct of that research over the past four years has yielded substantial new information about the genes and genetic markers that are associated with response to two important poultry pathogens, Salmonella enteritidis (SE) and Escherichia coli (EC), about variation of immunocompetence genes in poultry, about relationships of traits of immune response and production, and about interaction of genes with environment and with other genes and genetic background. The current BARD work has generated a base of knowledge and expertise regarding the genetic variation underlying the traits of immunocompetence and disease resistance. In addition, unique genetic resource populations of chickens have been established in the course of the current project, and they are essential for continued projects. The US laboratory has made considerable progress in studies of the genetics of resistance to SE. Microsatellite-marked chromosomal regions and several specific genes were linked to SE vaccine response or bacterial burden and the important phenomenon of gene interaction was identified in this system. In total, these studies demonstrate the role of genetics in SE response, the utility of the existing resource population, and the expertise of the research group in conducting such experiments. The Israeli laboratories had showed that the lines developed by selection for high or low level of antibody (Ab) response to EC differ similarly in Ab response to several other viral and bacterial pathogens, indicating the existence of a genetic control of general capacity of Ab response in young broilers. It was also found that the 10w-Ab line has developed, possibly via compensatory "natural" selection, higher cellular immune response. At the DNA levels, markers supposedly linked to immune response were identified, as well as SNP in the MHC, a candidate gene responsible for genetic differences in immunocompetence of chickens.
Los estilos APA, Harvard, Vancouver, ISO, etc.
9

Boisclair, Yves R. y Arieh Gertler. Development and Use of Leptin Receptor Antagonists to Increase Appetite and Adaptive Metabolism in Ruminants. United States Department of Agriculture, enero de 2012. http://dx.doi.org/10.32747/2012.7697120.bard.

Texto completo
Resumen
Objectives The original project had 2 major objectives: (1) To determine the effects of centrally administered leptin antagonist on appetite and adaptive metabolism in the sheep; (2) To develop and prepare second-generation leptin antagonists combining high binding affinity and prolonged in vivo half-life. Background Periods of suboptimal nutrition or exaggerated metabolic activity demands lead to a state of chronic energy insufficiency. Ruminants remain productive for a surprisingly long period of time under these circumstances by evoking adaptations sparing available energy and nutrients. The mechanism driving these adaptations in ruminant remains unknown, but could involve a reduction in plasma leptin, a hormone acting predominantly in the brain. In laboratory animals, reduced leptin signaling promotes survival during nutritional insufficiency by triggering energy sparing adaptations such as reduced thyroid hormone production and insulin resistance. Our overall hypothesis is that similar adaptations are triggered by reduced leptin signaling in the brain of ruminants. Testing of this hypothesis in ruminants has not been possible due to inability to block the actions of endogenous leptin and access to ruminant models where leptin antagonistic therapy is feasible and effective. Major achievements and conclusions The Israeli team had previously mutated 3 residues in ovine leptin, with no effect on receptor binding. This mutant was renamed ovine leptin antagonist (OLA) because it cannot activate signaling and therefore antagonizes the ability of wild type leptin to activate its receptor. To transform OLA into an effective in vivo antagonist, the Israeli made 2 important technical advances. First, it incorporated an additional mutation into OLA, increasing its binding affinity and thus transforming it into a super ovine leptin antagonist (SOLA). Second, the Israeli team developed a method whereby polyethylene glycol is covalently attached to SOLA (PEG-SOLA) with the goal of extending its half-life in vivo. The US team used OLA and PEG-SOLA in 2 separate animal models. First, OLA was chronically administered directly into the brain of mature sheep via a cannula implanted into the 3rdcerebroventricule. Unexpectedly, OLA had no effect of voluntary feed intake or various indicators of peripheral insulin action but reduced the plasma concentration of thyroid hormones. Second, the US team tested the effect of peripheral PEG-SOLA administration in an energy sensitive, rapidly growing lamb model. PEG-SOLA was administered for 14 consecutive days after birth or for 5 consecutive days before sacrifice on day 40 of life. Plasma PEG-SOLA had a half-life of over 16 h and circulated in 225- to 288-fold excess over endogenous leptin. PEG-SOLA administration reduced plasma thyroid hormones and resulted in a higher fat content in the carcass at slaughter, but had no effects on feed intake, body weight, plasma glucose or insulin. These results show that the team succeeded in developing a leptin antagonist with a long in vivo half-life. Moreover, in vivo results show that reduced leptin signaling promotes energy sparing in ruminants by repressing thyroid hormone production. Scientific and agricultural implications The physiological role of leptin in ruminants has been difficult to resolve because peripheral administration of wild type leptin causes little effects. Our work with leptin antagonists show for the first time in ruminants that reduced leptin signaling induces energy sparing mechanisms involving thyroid hormone production with little effect on peripheral insulin action. Additional work is needed to develop even more potent leptin antagonists, to establish optimal administration protocols and to narrow down phases of the ruminant life cycle when their use will improve productivity.
Los estilos APA, Harvard, Vancouver, ISO, etc.
10

Morrison, Mark y Joshuah Miron. Molecular-Based Analysis of Cellulose Binding Proteins Involved with Adherence to Cellulose by Ruminococcus albus. United States Department of Agriculture, noviembre de 2000. http://dx.doi.org/10.32747/2000.7695844.bard.

Texto completo
Resumen
At the beginning of this project, it was clear that R. albus adhered tightly to cellulose and its efficient degradation of this polysaccharide was dependent on micromolar concentrations of phenylacetic acid (PAA) and phenylpropionic acid (PPA). The objectives for our research were: i) to identify how many different kinds of cellulose binding proteins are produced by Ruminococcus albus; ii) to isolate and clone the genes encoding some of these proteins from the same bacterium; iii) to determine where these various proteins were located and; iv) quantify the relative importance of these proteins in affecting the rate and extent to which the bacterium becomes attached to cellulose. BARD support has facilitated a number of breakthroughs relevant to our fundamental understanding of the adhesion process. First, R. albus possesses multiple mechanisms for adhesion to cellulose. The P.I.'s laboratory has discovered a novel cellulose-binding protein (CbpC) that belongs to the Pil-protein family, and in particular, the type 4 fimbrial proteins. We have also obtained genetic and biochemical evidence demonstrating that, in addition to CbpC-mediated adhesion, R. albus also produces a cellulosome-like complex for adhesion. These breakthroughs resulted from the isolation (in Israel and the US) of spontaneously arising mutants of R. albus strains SY3 and 8, which were completely or partially defective in adhesion to cellulose, respectively. While the SY3 mutant strain was incapable of growth with cellulose as the sole carbon source, the strain 8 mutants showed varying abilities to degrade and grow with cellulose. Biochemical and gene cloning experiments have been used in Israel and the US, respectively, to identify what are believed to be key components of a cellulosome. This combination of cellulose adhesion mechanisms has not been identified previously in any bacterium. Second, differential display, reverse transcription polymerase chain reaction (DD RT-PCR) has been developed for use with R. albus. A major limitation to cellulose research has been the intractability of cellulolytic bacteria to genetic manipulation by techniques such as transposon mutagenesis and gene displacement. The P.I.'s successfully developed DD RT- PCR, which expanded the scope of our research beyond the original objectives of the project, and a subset of the transcripts conditionally expressed in response to PAA and PPA have been identified and characterized. Third, proteins immunochemically related to the CbpC protein of R. albus 8 are present in other R. albus strains and F. intestinalis, Western immunoblots have been used to examine additional strains of R. albus, as well as other cellulolytic bacteria of ruminant origin, for production of proteins immunochemically related to the CbpC protein. The results of these experiments showed that R. albus strains SY3, 7 and B199 all possess a protein of ~25 kDa which cross-reacts with polyclonal anti-CbpC antiserum. Several strains of Butyrivibrio fibrisolvens, Ruminococcus flavefaciens strains C- 94 and FD-1, and Fibrobacter succinogenes S85 produced no proteins that cross-react with the same antiserum. Surprisingly though, F. intestinalis strain DR7 does possess a protein(s) of relatively large molecular mass (~200 kDa) that was strongly cross-reactive with the anti- CbpC antiserum. Scientifically, our studies have helped expand the scope of our fundamental understanding of adhesion mechanisms in cellulose-degrading bacteria, and validated the use of RNA-based techniques to examine physiological responses in bacteria that are nor amenable to genetic manipulations. Because efficient fiber hydrolysis by many anaerobic bacteria requires both tight adhesion to substrate and a stable cellulosome, we believe our findings are also the first step in providing the resources needed to achieve our long-term goal of increasing fiber digestibility in animals.
Los estilos APA, Harvard, Vancouver, ISO, etc.
También puede estar interesado en las bibliografías ampliadas sobre el tema "Cats as laboratory animals" para tipos de fuentes particulares:
Artículos de revistas Tesis Libros
Ofrecemos descuentos en todos los planes premium para autores cuyas obras están incluidas en selecciones literarias temáticas. ¡Contáctenos para obtener un código promocional único!

Pasar a la bibliografía