Literatura académica sobre el tema "Capillary eletrophoresi"

碩士
國立交通大學
應用化學研究所
85
Capillary electrophoresis is one of important separation technique based on different migration velocities of analytes when a potential is applied. This method has many advantages including high separation efficiency, short analysis time, low consumption of sample, and easy automation. In recent years, the widespread use of antibiotics has resulted led to the emergence of antibiotic-resistant organisms. For this reason, some countries have emphasized on the misusing of antibiotics. Capillary electrophoresis was applied to separate two kinds of antibiotics in this paper. One is tetracyclines, another is β-lactam antibiotics. 　　Seven TCs were completely separated from each other. Also, the effect of adding EDTA and 1,3-Diaminopropane was discussed. The optimum separation condition then was applied to analyze milk. 　　The U.S. Food and Drug Administration (PDA) set "safe levels" for β-lactam antibiotics, including amoxicillin, ampicillin, ceftiofur, cephapirin, cloxacillin, and penicillin. In commercially, penicillin has two forms: penicillin G and penicillin V. In this study, micellar electrokinetic chromatography (MEKC) was developed for determining above seven β-lactam antibiotics. The effect of concentration of surfactant and buffer pH value on the retention behavior and selectivity would be discussed. The optimum separation condition then was applied to analyze animal medicine.

碩士
國立高雄師範大學
化學系
101
ABSTRACT In this study, unmodified magnetic Fe3O4 nanoparticles(NPs) were used for selective extraction and enrichment of flavonoids namely- catechin (C), epicatechin (EC), rutin (R), and quercitrin (Q), prior to their analysis using capillary electrophoresis with UV detection. Coordination of Fe3+ ions present on the surface of Fe3O4 to catechol with special interactions enables the magnetic NPs to capture the catechol moiety of the flavonoids from an aqueous solution. Under the optimum extraction conditions, the limit of detections at signal-to-noise of 3:1 for C, EC, R and Q were found to be 0.82, 0.76, 0.02 and 0.02 μM, respectively. enrichment factor of up to 156.04 - 451.79. Notably, the C18 solid-phase extraction (SPE) combined with Fe3O4 NPs Magnetic solid phase extraction and the proposed method was successfully applied for the determination of C, EC, and Q in the human urine. The accuracy of this method was evaluated by the recovery of analytes in the spiked sample. Good recoveries (99.36-102.66%) with low relative standard deviations (2.70 to 3.89%) were achieved. Some advantages of the proposed methods have high sensitivity, high precision, and high accuracy.