Artículos de revistas: "C812"

1

Baxter, Brady. "Management of chronic iron overload". Paediatric Care 14, n.º 7 (septiembre de 2002): 14–16. http://dx.doi.org/10.7748/paed2002.09.14.7.14.c812.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

2

Brodsky, J. L. "Insulin activation of brain Na(+)-K(+)-ATPase is mediated by alpha 2-form of enzyme". American Journal of Physiology-Cell Physiology 258, n.º 5 (1 de mayo de 1990): C812—C817. http://dx.doi.org/10.1152/ajpcell.1990.258.5.c812.

Texto completo

Resumen

The sensitivity of the synaptosomal Na(+)-K(+)-ATPase to insulin was examined and found to be stimulated by the hormone when physiological intracellular sodium concentrations were present. Activation was not mediated by a sodium influx into the vesicles, as shown using sodium uptake experiments and by the fact that tetrodotoxin did not inhibit insulin action. Because the brain Na(+)-K(+)-ATPase catalytic subunit exists as two forms with different affinities for the inhibitory cardiac glycoside ouabain, the sensitivity of each form for insulin was examined. As previously observed in adipocytes, only the high-affinity component, alpha 2, was insulin sensitive. A dose-response curve of insulin activation of the Na(+)-K(+)-ATPase demonstrated a maximal insulin effect at relatively high hormone concentrations. It is unknown, therefore, whether stimulation of the brain Na(+)-K(+)-ATPase occurs in vivo.

Los estilos APA, Harvard, Vancouver, ISO, etc.

3

Janigro, D., G. A. West, E. L. Gordon y H. R. Winn. "ATP-sensitive K+ channels in rat aorta and brain microvascular endothelial cells". American Journal of Physiology-Cell Physiology 265, n.º 3 (1 de septiembre de 1993): C812—C821. http://dx.doi.org/10.1152/ajpcell.1993.265.3.c812.

Texto completo

Resumen

The endothelium plays an important role in the modulation of vascular tone and blood cell activation. Extensive work has demonstrated that the release of endothelium-derived relaxing factor (EDRF) from the endothelium is evoked by a number of physical and chemical stimuli requiring Ca2+. Because endothelial cells do not express voltage-dependent Ca2+ channels, Ca2+ influxes following receptor activation may be facilitated by cell hyperpolarizations mediated by the activation of K+ conductances. There has been recent interest in the role of ATP-sensitive K+ channels (KATP) suggesting that KATP may play a role in the regulation of blood flow. We have investigated the electrophysiological properties of an ATP-sensitive K+ conductance in whole cell and membrane patches from rat aorta and brain microvascular endothelial cells. Whole cell as well as single-channel currents were increased by either intracellular dialysis of ATP or application of glucose-free/NaCN (2 mM) solutions. Both currents were reversibly blocked by glibenclamide (1-100 microM). The KATP channel opener pinacidil (30 microM) caused activation of an outward current in the presence of physiological intracellular ATP concentrations. In inside-out patches, 10 microM-1 mM ATP invariably caused a dramatic decrease in channel activity. We conclude that both rat aorta and brain microvascular endothelial cells express KATP channels. KATP may play a role in the regulation of endothelial cell resting potential during impaired energy supply and therefore modulate EDRF release and thus cerebral blood flow.

Los estilos APA, Harvard, Vancouver, ISO, etc.

4

Valenti, G., J. M. Verbavatz, I. Sabolic, D. A. Ausiello, A. S. Verkman y D. Brown. "A basolateral CHIP28/MIP26-related protein (BLIP) in kidney principal cells and gastric parietal cells". American Journal of Physiology-Cell Physiology 267, n.º 3 (1 de septiembre de 1994): C812—C820. http://dx.doi.org/10.1152/ajpcell.1994.267.3.c812.

Texto completo

Resumen

The water channel CHIP28 accounts for the high water permeability of proximal tubules and thin descending limbs of Henle; a homologous water channel, WCH-CD, in the apical membrane of collecting duct principal cells, may be the vasopressin-sensitive water channel. We show here that one antiserum, raised against CHIP28, immunostains the basolateral membrane of collecting duct principal cells, in addition to staining CHIP28 in other cells. This serum was named anti-basolateral integral protein (anti-BLIP) to distinguish it from other anti-CHIP28 antisera. By Western blotting, BLIP serum recognized both CHIP28 and MIP26, and it stained lens fibers, which contain MIP26 but not CHIP28. BLIP antiserum immunoprecipitated a 28-kDa band, a broad 35- to 50-kDa band, and an approximately 16-kDa band from kidney papilla. It also stained the basolateral membrane of gastric parietal cells, which were not stained with anti-CHIP28 or anti-MIP26 antibodies. BLIP antiserum immunoprecipitated a 28-kDa protein band from stomach; this protein was not precipitated by anti-CHIP28 antibodies. These results suggest that basolateral membranes of principal cells and parietal cells contain a protein(s) that shares common epitopes with CHIP28 and MIP26. Finally, BLIP but not CHIP28 antiserum stained mesothelial (but not epithelial) cells of toad urinary bladder, a further indication that the BLIP antiserum recognizes a protein distinct from CHIP28.

Los estilos APA, Harvard, Vancouver, ISO, etc.

5

Hattori, M., K. Sakamoto, N. Fujihara y I. Kojima. "Nitric oxide: a modulator for the epidermal growth factor receptor expression in developing ovarian granulosa cells". American Journal of Physiology-Cell Physiology 270, n.º 3 (1 de marzo de 1996): C812—C818. http://dx.doi.org/10.1152/ajpcell.1996.270.3.c812.

Texto completo

Resumen

the present study was designed to assess the involvement of nitric oxide (NO) in the regulation of the epidermal growth factor (EGF) receptor during development of rat granulosa cells, which were prepared by puncturing ovaries of diethylstilbestrol-treated rats. The immature cells were cultured for 48 h with follicle-stimulating hormone (FSH) to be transformed into mature cells. A marked accumulation of guanosine 3',5' -cyclic monophosphate (cGMP) was observed during development. The accumulation of cGMP, but not of adenosine 3',5'-cyclic monophosphate (cAMP), was suppressed by specific inhibitors of NO synthase, L-NG-monomethyl-L-arginine (L-NMMA) and L-N(G)-nitro-L-arginine, and a selective inhibitor of the inducible NO synthase, aminoguanidine. The L-NMMA-induced suppression was partially reversed by addition of L-arginine to cultures but not D-arginine, indicating that NO formation is inhibited by competing with analogues of L-arginine for NO synthase. Treatment with 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide , an antagonist of NO, caused suppression in the increase of EGF binding sites, whereas exposure of the cells to sodium nitroprusside (SNP), an NO donor, caused elevation in EGF binding sites, with increasing extra- and intracellular cGMP levels. Analysis of the EGF receptor by affinity labeling with 125I-labeled EGF revealed that the intensity of the cross-linked receptor molecular with a molecular mass of 180 kDa was increased by exposure to SNP. The facilitatory effect of SNP on the EGF receptor was observed when the cAMP-dependent pathway was fully activated by FSH. However, the NO effect may be mediated by a cGMP-independent pathway, as 8-bromo-cGMP did not mimic the action of SNP. These results indicate that the L-arginine-NO system may contribute to the regulation of EGF receptor expression in developing granulosa cells stimulated by FSH.

Los estilos APA, Harvard, Vancouver, ISO, etc.

6

Fischer, Silvia, Matthias Clauss, Marion Wiesnet, Dieter Renz, Wolfgang Schaper y Gerhard F. Karliczek. "Hypoxia induces permeability in brain microvessel endothelial cells via VEGF and NO". American Journal of Physiology-Cell Physiology 276, n.º 4 (1 de abril de 1999): C812—C820. http://dx.doi.org/10.1152/ajpcell.1999.276.4.c812.

Texto completo

Resumen

In this study, an in vitro model of the blood-brain barrier, consisting of porcine brain-derived microvascular endothelial cells (BMEC), was used to evaluate the mechanism of hypoxia-induced hyperpermeability. We show that hypoxia-induced permeability in BMEC was completely abolished by a neutralizing antibody to vascular endothelial growth factor (VEGF). In contrast, under normoxic conditions, addition of VEGF up to 100 ng/ml did not alter monolayer barrier function. Treatment with either hypoxia or VEGF under normoxic conditions induced a twofold increase in VEGF binding sites and VEGF receptor 1 (Flt-1) mRNA expression in BMEC. Hypoxia-induced permeability also was prevented by the nitric oxide (NO) synthase inhibitor NG-monomethyl-l-arginine, suggesting that NO is involved in hypoxia-induced permeability changes, which was confirmed by measurements of the cGMP level. During normoxia, treatment with VEGF (5 ng/ml) increased permeability as well as cGMP content in the presence of several antioxidants. These results suggest that hypoxia-induced permeability in vitro is mediated by the VEGF/VEGF receptor system in an autocrine manner and is essentially dependent on reducing conditions stabilizing the second messenger NO as the mediator of changes in barrier function of BMEC.

Los estilos APA, Harvard, Vancouver, ISO, etc.

7

da Costa, Maria, Sheldon P. Rothenberg, Easwara Sadasivan, Annette Regec y Lian Qian. "Folate deficiency reduces the GPI-anchored folate-binding protein in rat renal tubules". American Journal of Physiology-Cell Physiology 278, n.º 4 (1 de abril de 2000): C812—C821. http://dx.doi.org/10.1152/ajpcell.2000.278.4.c812.

Texto completo

Resumen

A folate-binding protein (FBP) anchored to cell membranes by a glycosyl phosphatidylinositol (GPI) adduct is constitutively expressed in some transformed and cultured cell lines. Its expression is upregulated when these cells are grown in medium containing low folate, but whether this occurs in vivo with nutritional folate deficiency is unknown. To address this question, the GPI-FBP in the liver, kidney, and brain of rats on control and folate-deficient (FD) diets was measured. The GPI-FBP in the kidney of FD rats decreased significantly in contrast to the upregulation of this protein in cultured cells. Northern blot analysis and nuclear run-on assays indicated that transcription of the GPI-FBP gene in the kidney was not reduced by folate deficiency. This decrease of the GPI-FBP appears to result from its proteolysis, similar to the enzymatic degradation of the apoprotein that occurs in vitro. Because the GPI-FBP is on the brush borders of the proximal renal tubules and provides for the reabsorption of folate, this function diminishes when the protein decreases in folate deficiency.

Los estilos APA, Harvard, Vancouver, ISO, etc.

8

Vasquez Chavez, Jule Franve, Arturo Zapata, Juan Luis Garcia, Carlos Barrionuevo, Edgar Diaz, Ebert Poquioma, Tatiana Vidaurre y Luis Malpica. "Incidence and survival of Hodgkin lymphoma patients treated at a national cancer center in a middle-income country from 1999-2018: A report of 1,382 cases." Journal of Clinical Oncology 39, n.º 15_suppl (20 de mayo de 2021): e19523-e19523. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.e19523.

Texto completo

Resumen

e19523 Background: Hodgkin lymphoma (HL) is classically described as a malignancy with a good prognosis and bimodal pattern. We aim to describe the incidence and survival of HL patients treated at the National Cancer Institute (NCI) in Peru. Methods: We evaluated the database at the NCI Epidemiology Department in Peru from 1999-2018. The international classification of diseases (ICD)-10 codes C810-C813, C817, C819 and ICD for Oncology (version 3) codes 96503-96533, 96573-96593, 96533-96553, 96573, 99903 were used to classify both nodal and extra-nodal HL. The estimate of overall survival (OS) curves was performed by the Kaplan-Meier method, and the difference was computed by the log-rank test. Results: During the study period 12,092 patients with lymphoma were found, of which 1,382 (11.4%) were HL. The median age was 24 years (range 2-88). Male patients were 62.4%. Patients of ≤14 years were 32.9%, with the most frequent 5-year interval group those aged 5-9 years-old representing 16.7% of patients. The adolescent and young adults (AYA) (15-39 years) group represented 38.4%. A total of 1,360 (98.4%) were classified as nodal HL. The classification according to the subtypes was as follows: 1,148 (83.1%) were one of the five subtypes [classical HL (cHL) and nodular lymphocyte-predominant HL (NLPHL)], 196 (14.2%) were HL, not otherwise specified, and 38 (2.7%) were HL without pathological confirmation. The classifiable 1,148 patients were categorized as follows: 1,107 patients (96.4%) were cHL and 41 (3.6%) were NLPHL. The cHL patients were classified as mixed cellularity HL (MCHL) in 52.3%, nodular sclerosis HL (NSHL) in 36.7%, lymphocyte-rich HL (LRHL) in 8.1%, and lymphocyte-depleted HL (LDHL) in 2.9%. The whole classifiable cohort (n = 1,148) showed a trimodal pattern. The MCHL subtype was most frequent in the 5-9 years group and in the 60-64 years group. The NSHL subtype was most frequent in the 20-24 years group. The median 5-year OS of the entire cohort (n = 1,382) was 86.3% (95%CI, 83.9-88.5). The OS according to the interval age groups were as follows: for the 0-14 years group the 5-yr OS was 94.9% (95%CI, 91.8-96.8), for the 15-39 years was 84% (95%CI, 79.5-87.68), for the 40-64 years group was 82.5% (95%CI, 75.2-87.8), and for the ≥65 years group was 58.6% (95%CI, 43.4-71.1). The OS according to age range showed a statistically significant difference p < 0.01. Conclusions: In our study, patients with HL are younger compared to those in developed countries. The most frequent 5-year interval group is the 5-9 years group. HL incidence had a trimodal pattern. Extra-nodal presentation was extremely rare. NLPHL was also rare. The most frequent subtype was MCHL, however, in AYA patients the most frequent subtype was NSHL. Better OS was seen in the 0-14 year group, this being statistically significant compared to the older groups. Worse OS was observed in patients aged 65 and older.

Los estilos APA, Harvard, Vancouver, ISO, etc.

9

Ashenfelter, Orley. "Comparing Real Wage Rates". American Economic Review 102, n.º 2 (1 de abril de 2012): 617–42. http://dx.doi.org/10.1257/aer.102.2.617.

Texto completo

Resumen

A real wage rate is a nominal wage rate divided by the price of a good and is a transparent measure of how much of the good an hour of work buys. It provides an important indicator of the living standards of workers, and also of the productivity of workers. In this paper I set out the conceptual basis for such measures, provide some historical examples, and then provide my own preliminary analysis of a decade long project designed to measure the wages of workers doing the same job in over 60 countries—workers at McDonald's restaurants. The results demonstrate that the wage rates of workers using the same skills and doing the same jobs differ by as much as 10 to 1, and that these gaps declined over the period 2000–2007, but with much less progress since the Great Recession. (JEL C81, C82, D24, J31, N30, O57)

Los estilos APA, Harvard, Vancouver, ISO, etc.

10

Demir, Semahat S., John W. Clark y Wayne R. Giles. "Parasympathetic modulation of sinoatrial node pacemaker activity in rabbit heart: a unifying model". American Journal of Physiology-Heart and Circulatory Physiology 276, n.º 6 (1 de junio de 1999): H2221—H2244. http://dx.doi.org/10.1152/ajpheart.1999.276.6.h2221.

Texto completo

Resumen

We have extended our compartmental model [ Am. J. Physiol. 266 ( Cell Physiol. 35): C832–C852, 1994] of the single rabbit sinoatrial node (SAN) cell so that it can simulate cellular responses to bath applications of ACh and isoprenaline as well as the effects of neuronally released ACh. The model employs three different types of muscarinic receptors to explain the variety of responses observed in mammalian cardiac pacemaking cells subjected to vagal stimulation. The response of greatest interest is the ACh-sensitive change in cycle length that is not accompanied by a change in action potential duration or repolarization or hyperpolarization of the maximum diastolic potential. In this case, an ACh-sensitive K+ current is not involved. Membrane hyperpolarization occurs in response to much higher levels of vagal stimulation, and this response is also mimicked by the model. Here, an ACh-sensitive K+ current is involved. The well-known phase-resetting response of the SAN cell to single and periodically applied vagal bursts of impulses is also simulated in the presence and absence of the β-agonist isoprenaline. Finally, the responses of the SAN cell to longer continuous trains of periodic vagal stimulation are simulated, and this can result in the complete cessation of pacemaking. Therefore, this model is 1) applicable over the full range of intensity and pattern of vagal input and 2) can offer biophysically based explanations for many of the phenomena associated with the autonomic control of cardiac pacemaking.

Los estilos APA, Harvard, Vancouver, ISO, etc.

11

Wang, Jian-Qiang y Thomas F. Fässler. "The Anion [Co(C8H12)2]–. A Comparative Study of the Crystal Structures of [{K(18-crown-6)}2(C5H5)][Co(C8H12)2] and Co(C8H12)(C8H13)". Zeitschrift für Naturforschung B 64, n.º 8 (1 de agosto de 2009): 985–88. http://dx.doi.org/10.1515/znb-2009-0815.

Texto completo

Resumen

The cobalt complex [{K(18-crown-6)}2(C5H5)]- [Co(C8H12)2]・(THF)2 (3) has been synthesized and characterized by X-ray single-crystal structure determination. The crystal structure of Co(C8H12)(C8H13) (2) has been reinvestigated and compared with the structure of 3. The 1,5-cyclooctadiene (C8H12) and C8H13 ligands are coordinated in an η4 and η3 fashion, respectively. The cyclopentadienyl anion in [{K(18-crown-6)}2(C5H5)]+ in 3 is η5-coordinated to the two crown ether-encapsulated potassium cations

Los estilos APA, Harvard, Vancouver, ISO, etc.

12

Dong, Wei, Qin Zhou, Wangqiang Shen, Le Yang, Peng Jin, Xing Lu y Yongfu Lian. "The Various Packing Structures of Tb@C82 (I, II) Isomers in Their Cocrystals with Ni(OEP)". Nanomaterials 13, n.º 6 (9 de marzo de 2023): 994. http://dx.doi.org/10.3390/nano13060994.

Texto completo

Resumen

Soot-containing terbium (Tb)-embedded fullerenes were prepared by evaporation of Tb4O7-doped graphite rods in an electric arc discharge chamber. After 1,2,4-trichlorobenzene extraction of the soot and rotary evaporation of the extract, a solid product was obtained and then dissolved into toluene by ultrasonication. Through a three-stage high-pressure liquid chromatographic (HPLC) process, Tb@C82 (I, II) isomers were isolated from the toluene solution of fullerenes and metallofullerenes. With the success of the growth of cocrystals of Tb@C82 (I, II) with Ni(OEP), the molecular structures of Tb@C82 (I) and Tb@C82 (II) were confirmed to be Tb@C2v(9)-C82 and Tb@Cs(6)-C82, respectively, based on crystallographic data from X-ray single-crystal diffraction. Moreover, it was found that Tb@C82 (I, II) isomers demonstrated different packing behaviors in their cocrystals with Ni(OEP). Tb@C2v(9)-C82 forms a 1:1 cocrystal with Ni(OEP), in which Tb@C2v(9)-C82 is aligned diagonally between the Ni(OEP) bilayers to form zigzag chains. In sharp contrast, Tb@Cs(6)-C82 forms a 2:2 cocrystal with Ni(OEP), in which Tb@Cs(6)-C82 forms a centrosymmetric dimer that is aligned linearly with Ni(OEP) pairs to form one-dimensional structures in the a–c lattice plane. In addition, the distance of a Ni atom in Ni(OEP) to the Cs(6)-C82 cage is much shorter than that to the C2v(9)-C82 one, indicative of a stronger π-π interaction between Ni(OEP) and the C82 carbon cage in the cocrystal of Tb@CS(6)-C82 and Ni(OEP). Density functional theory calculations reveal that the regionally selective dimerization of Tb@CS(6)-C82 is the result of a dominant unpaired spin existing on a particular C atom of the CS(6)-C82 cage.

Los estilos APA, Harvard, Vancouver, ISO, etc.

13

Zhou, Xinyi, Yang-Rong Yao, Yajing Hu, Le Yang, Shaoting Yang, Yilu Zhang, Qianyan Zhang, Ping Peng, Peng Jin y Fang-Fang Li. "Reactivity of Open-Shell Metallofullerene Anions: Synthesis, Crystal Structures, and Electrochemical Properties of Benzylated Gd@C2v-C82". Inorganics 11, n.º 9 (25 de agosto de 2023): 349. http://dx.doi.org/10.3390/inorganics11090349.

Texto completo

Resumen

The reactivity of the open-shell Gd@C2v-C82 with different charge states towards benzyl bromide was investigated. [Gd@C2v-C82]3− exhibited enhanced activity relative to Gd@C2v-C82 and [Gd@C2v-C82]−. The structural characterizations, including MALDI-TOF MS, UV-vis-NIR, and single crystal X-ray diffraction, indicate the formation of isomeric benzyl monoadducts of Gd@C2v-C82. All three monoadducts contain 1:1 mirror-symmetric enantiomers. Additionally, the addition of the benzyl group and its specific position result in distinct electrochemical behavior of the products compared to the parent Gd@C2v-C82. Theoretical studies demonstrate that only [Gd@C2v-C82]3− has a HOMO energy level that matches well with the LUMO energy level of the PhCH2 radical, providing a rationalization for the observed significantly different reactivity.

Los estilos APA, Harvard, Vancouver, ISO, etc.

14

Blondel, Marc, Jean-Marc Galan y Matthias Peter. "Isolation and Characterization of HRT1 Using a Genetic Screen for Mutants Unable to Degrade Gic2p in Saccharomyces cerevisiae". Genetics 155, n.º 3 (1 de julio de 2000): 1033–44. http://dx.doi.org/10.1093/genetics/155.3.1033.

Texto completo

Resumen

Abstract Skp1p-cullin-F-box (SCF) protein complexes are ubiquitin ligases required for degradation of many regulatory proteins involved in cell cycle progression, morphogenesis, and signal transduction. Using a genetic screen, we have isolated a novel allele of the HRT1/RBX1 gene in budding yeast (hrt1-C81Y). hrt1-C81Y mutant cells exhibited an aberrant morphology but were viable at all temperatures. The cells displayed multiple genetic interactions with mutations in known SCF components and were defective for the degradation of several SCF targets including Gic2p, Far1p, Sic1p, and Cln2p. In addition, they also failed to degrade the F-box proteins Grr1p, Cdc4p, and Met30p. Wild-type Hrt1p but not Hrt1p-C81Y was able to bind multiple F-box proteins in an F-box-dependent manner. Hrt1p-C81Y harbors a single mutation in its ring-finger domain, which is conserved in subunits of distinct E3 ligases. Finally, Hrt1p was localized in both nucleus and cytoplasm and despite a short half-life was expressed constitutively throughout the cell cycle. Taken together, these results suggest that Hrt1p is a core subunit of multiple SCF complexes.

Los estilos APA, Harvard, Vancouver, ISO, etc.

15

Fenner, Ulrich, Hans Pritzkow y Walter Siebert. "Synthese und Komplexierung des 4.5-Cycloocta-2,3-dihydro-1,2,3-trimethyl-1,3-diborols / Synthesis and Complexation of 4,5-Cycloocta-2,3-dihydro-1,2,3-trimethyl-1,3-diborole". Zeitschrift für Naturforschung B 49, n.º 3 (1 de marzo de 1994): 315–20. http://dx.doi.org/10.1515/znb-1994-0304.

Texto completo

Resumen

Abstract Redox reactions of cyclooctyne and 1,1-bis(diiodboryl)ethane lead to 4,5-cycloocta-2,3-dihydro-1,3-diiodo-2-methyl-1,3-diborole (1a), which is methylated with AlMe3 to give 4,5-cycloocta-2,3-dihydro-1,2,3-trimethyl-1,3-diborole (1b). Complexation of 1b with the complex fragments (C5H5)Co, (C8H12)Ni, (C5H5)Ni, and (OC)3Co leads to the corresponding metal complexes 2b, 3b, 4b, and 5b. Their constitutions are derived from spectroscopic data and confirmed for 2b and 4b by X-ray structure analyses. 2b contains a C-H-B 3c/2e bond, its hydrogen atom could be located. [(C8H12)Ni(1b)] (3b) is the first example for a (C8H12)Ni complex having a C-H-B interaction.

Los estilos APA, Harvard, Vancouver, ISO, etc.

16

Doglio, L. T., M. S. Gawryl y T. F. Lint. "Analysis of human C8 with monoclonal antibodies. Characterization of a monoclonal antibody that recognizes free C8 alpha-gamma subunit." Journal of Immunology 141, n.º 6 (15 de septiembre de 1988): 2079–83. http://dx.doi.org/10.4049/jimmunol.141.6.2079.

Texto completo

Resumen

Abstract The eighth component of human C is essential for the formation of the membranolytic C attack complex. C8 has a unique structure in that two covalently linked chains, C8 alpha and C8 gamma, are associated non-covalently with the third chain, C8 beta. In order to study the structure and assembly of the C8 molecule, a panel of mAb has been produced against the C component C8. Eight of these mAb had reactivity to the C8 alpha-gamma subunit, whereas four reacted with C8 beta. One of the C8 alpha-gamma mAb, C8A2, had specificity for an epitope on the C8 alpha-chain and exhibited no cross-reactivity to any of the other terminal C components, including C8 beta. C8A2 inhibited the hemolytic activity of the C8 alpha-gamma subunit but had no effect on the activity of fluid phase whole C8 or C8 within membrane-bound C5b-8. Functional experiments suggest that C8A2 inhibits C8 alpha-gamma activity by interfering with its interaction with the C8 beta-chain. In an enzyme immunoassay using the C8A2 mAb, free C8 alpha-gamma subunit could be detected in both homozygous and heterozygous C8 beta-deficient serum. However, only low level binding was observed when homozygous C5- and C7-deficient sera were tested. Thus the mAb, C8A2, recognizes an epitope expressed on the C8 alpha-gamma subunit but not on intact C8 and can detect free C8 alpha-gamma in the presence of native C8.

Los estilos APA, Harvard, Vancouver, ISO, etc.

17

Maeda, Yutaka, Saeka Akita, Mitsuaki Suzuki, Michio Yamada, Takeshi Akasaka, Kaoru Kobayashi y Shigeru Nagase. "Controlling the reactivity of La@C₈₂ by reduction: reaction of the La@C₈₂ anion with alkyl halide with high regioselectivity". Beilstein Journal of Organic Chemistry 19 (11 de diciembre de 2023): 1858–66. http://dx.doi.org/10.3762/bjoc.19.138.

Texto completo

Resumen

Endohedral metallofullerenes have excellent redox properties, which can be used to vary their reactivity to certain classes of molecules, such as alkyl halides. In this study, the thermal reaction of the La@C2v-C82 anion with benzyl bromide derivatives 1 at 110 °C afforded single-bonded adducts 2–5 with high regioselectivity. The products were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and visible–near infrared spectroscopy. The reaction of La@C2v-C82 with alkyl halides using the same conditions showed no consumption of La@C2v-C82, indicating that the reactivity of La@C2v-C82 toward alkyl halides was effectively increased by one-electron reduction. Single-crystal X-ray diffraction analysis of the single-bonded adduct 3a revealed the addition site of the p-methoxybenzyl group on La@C2v-C82. Theoretical calculations indicated that the addition site carbons in neutral La@C2v-C82 have high spin density, whereas those in the La@C2v-C82 anion do not have high charge densities. Thus, the reaction is believed to occur via electron transfer, followed by the radical coupling of La@C2v-C82 and benzyl radicals, rather than by bimolecular nucleophilic substitution reaction of La@C2v-C82 anion with 1.

Los estilos APA, Harvard, Vancouver, ISO, etc.

18

Yasutake, Yuhsuke, Zujin Shi, Toshiya Okazaki, Hisanori Shinohara y Yutaka Majima. "Interaction Control Between Endohedral Metallofullerene and Metal Substrate by Introducing Alkanethiol Self-Assembled Monolayer". Journal of Nanoscience and Nanotechnology 6, n.º 11 (1 de noviembre de 2006): 3460–63. http://dx.doi.org/10.1166/jnn.2006.034.

Texto completo

Resumen

The interaction control between endohedral metallofullerenes and a metal substrate has been demonstrated by introducing hexanethiol, octanethiol, and decanethiol self-assembled monolayers (SAMs) as the interlayer. We observe the electric properties of terbium endohedral metallofullerenes (Tb@C82) on alkanethiol SAMs with different chain lengths by scanning tunneling microscopy (STM) and spectroscopy (STS). Based on the comparison of the high-resolution STM images of a Tb@C82 molecule on hexanethiol and octanethiol SAMs, the interaction between Tb@C82 and a hexanethiol SAM is found to be larger than that between Tb@C82 and an octanethiol SAM; this is because at 68 K, the rotational states of Tb@C82 terminate only on the hexanethiol SAM. Furthermore, we find that the tunneling current-voltage characteristics of Tb@C82 on the hexanethiol SAM show the rectifying effects that are also caused by the molecular energy level shifts of Tb@C82 molecules due to the large interaction.

Los estilos APA, Harvard, Vancouver, ISO, etc.

19

Nishizawa, Masako, Masakazu Kamata, Ryoichi Katsumata y Yoko Aida. "A Carboxy-Terminally Truncated Form of the Human Immunodeficiency Virus Type 1 Vpr Protein Induces Apoptosis via G1 Cell Cycle Arrest". Journal of Virology 74, n.º 13 (1 de julio de 2000): 6058–67. http://dx.doi.org/10.1128/jvi.74.13.6058-6067.2000.

Texto completo

Resumen

ABSTRACT Viral protein R (Vpr) of human immunodeficiency virus type 1 inhibits cell proliferation by arresting the cell cycle at the G2 phase and inducing to apoptosis after G2arrest. We have reported previously that C81, a carboxy-terminally truncated form of Vpr, interferes with cell proliferation via a novel pathway that is distinct from G2 arrest. However, the mechanism of this effect of C81 is unknown. We demonstrate here that C81 can induce apoptosis via G1 arrest of the cell cycle. Immunostaining for various markers of stages of the cell cycle and flow cytometry analysis of DNA content showed that most HeLa cells that had been transiently transfected with a C81 expression vector were arrested at the G1 phase and not at the G2 or S phase of the cell cycle. Staining for annexin V, which binds phosphatidylserine on the plasma membrane, as an early indicator of apoptosis and measurement of the activity of caspase-3, a signaling molecule in apoptotic pathways, indicated that C81 is a strong inducer of apoptosis. Expression of C81 induced the condensation, fragmentation, and clumping of chromatin that are typical of apoptosis. Furthermore, the kinetics of the C81-induced G1 arrest were closely correlated with changes in the number of annexin V-positive cells and the activity of caspase-3. Replacement of Ile or Leu residues by Pro at positions 60, 67, 74, and 81 within the leucine zipper-like domain of C81 revealed that Ile60, Leu67, and Ile74 play important roles both in the C81-induced G1 arrest and in apoptosis. Thus, it appears that C81 induces apoptosis through pathways that are identical to those utilized for G1 arrest of the cell cycle. It has been reported that Ile60, Leu67, and Ile74 also play an important role in the C81-induced suppression of growth. These results suggest that the suppression of growth induced by C81 result in apoptosis that is independent of G2 arrest of the cell cycle.

Los estilos APA, Harvard, Vancouver, ISO, etc.

20

Lutnaes, Bjart F., Jostein Krane, Ben E. Smith y Steven J. Rowland. "Structure elucidation of C80, C81 and C82 isoprenoid tetraacids responsible for naphthenate deposition in crude oil production". Organic & Biomolecular Chemistry 5, n.º 12 (2007): 1873. http://dx.doi.org/10.1039/b701462g.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

21

Нестеренко, Олександр. "НАЦІОНАЛЬНІ ІНФОРМАЦІЙНІ РЕСУРСИ: МИНУЛЕ І СЬОГОДЕННЯ, АБО ДВАДЦЯТЬ РОКІВ ПОТОМУ". Society. Economy. Digitalization 1, n.º 1 (1 de mayo de 2024): 45–59. http://dx.doi.org/10.31379/sed.1.1.2024.2.

Texto completo

Resumen

Метою дослідження є визначення інформаційно-технологічних та методологічних шляхів вирішення актуальних проблем забезпечування підвищення рівня розвитку національних інформаційних ресурсів в новій цифровій реальності. Проведено огляд публікацій світової наукової спільноти на основі пошуку з використанням сервісу Google books Ngram Viewer, а також у вітчизняних та міжнародних наукометричних базах даних, таких як ресурси Національної бібліотеки України імені В.І. Вернадського, Scopus та Web of Science. Цей огляд має на увазі такі дослідницькі питання: а) які часові тренди демонструє статистика досліджень; б) які сфери створення та використання інформаційних ресурсів розглядаються у дослідженнях; в) які державні стратегії відображені в публікаціях для подолання проблем розвитку національних інформаційних ресурсів. Основні результати свідчать, що дослідники приділяють увагу питанням розвитку національних інформаційних ресурсів в різних секторах діяльності, але переважно бібліотечним ресурсам, а також в сфері медицини, охорони здоров’я, генетики, біохімії, молекулярної біології тощо. Водночас оцінювання сучасного стану національних інформаційних ресурсів в країні і напрямів їх розвію свідчить про існуючий розрив між потребами суспільства та індустрією ресурсів, а також про недостатність уваги щодо питань державного управління сферою НІР. Систематичні огляди літератури, подібні до проведеного, можуть бути основою формування інформаційних метаресурсів, що утримуються інформаційними системами відповідних державних установ. Ці ресурси можуть використовуватись не лише відповідальними особами для підтримки прийняття рішень щодо формування та розвитку НІР, а й безпосередньо фахівцями різних галузей в процесі формування та актуалізації корпусу тематичних інформаційних ресурсів. JEL Класифікація: C81; C82; D83; L86

Los estilos APA, Harvard, Vancouver, ISO, etc.

22

Wang, Xiuxiu, Nan Yang, Juan Su, Chenchen Wu, Shengtang Liu, Lei Chang, Leigh D. Plant y Xuanyu Meng. "The Molecular Mechanism of Human Voltage-Dependent Anion Channel 1 Blockade by the Metallofullerenol Gd@C82(OH)22: An In Silico Study". Biomolecules 12, n.º 1 (12 de enero de 2022): 123. http://dx.doi.org/10.3390/biom12010123.

Texto completo

Resumen

The endohedral metallofullerenol Gd@C82(OH)22 has been identified as a possible antineoplastic agent that can inhibit both the growth and metastasis of cancer cells. Despite these potentially important effects, our understanding of the interactions between Gd@C82(OH)22 and biomacromolecules remains incomplete. Here, we study the interaction between Gd@C82(OH)22 and the human voltage-dependent anion channel 1 (hVDAC1), the most abundant porin embedded in the mitochondrial outer membrane (MOM), and a potential druggable target for novel anticancer therapeutics. Using in silico approaches, we observe that Gd@C82(OH)22 molecules can permeate and form stable interactions with the pore of hVDAC1. Further, this penetration can occur from either side of the MOM to elicit blockage of the pore. The binding between Gd@C82(OH)22 and hVDAC1 is largely driven by long-range electrostatic interactions. Analysis of the binding free energies indicates that it is thermodynamically more favorable for Gd@C82(OH)22 to bind to the hVDAC1 pore when it enters the channel from inside the membrane rather than from the cytoplasmic side of the protein. Multiple factors contribute to the preferential penetration, including the surface electrostatic landscape of hVDAC1 and the unique physicochemical properties of Gd@C82(OH)22. Our findings provide insights into the potential molecular interactions of macromolecular biological systems with the Gd@C82(OH)22 nanodrug.

Los estilos APA, Harvard, Vancouver, ISO, etc.

23

Setiawati, Mieke Rochimi, Listiani Sugiyono, Nadia Nuraniya Kamaluddin y Tualar Simarmata. "The use of endophytic growth-promoting bacteria to alleviate salinity impact and enhance the chlorophyll, N uptake, and growth of rice seedling". Open Agriculture 6, n.º 1 (1 de enero de 2021): 798–806. http://dx.doi.org/10.1515/opag-2021-0059.

Texto completo

Resumen

Abstract Soil salinity is a major limiting factor for crop productivity, which increases continuously due to climate change. This barrier can possibly be overcome with the occurrence of halotolerant endophytic bacteria which reportedly plays an important role in protecting plants against various environmental stresses. Therefore, plant growth-promoting microbes are used in agriculture as an inexpensive and eco-friendly technology to enhance crop productivity in saline areas. In this study, the three isolates with nitrogen fixation ability were applied for mitigation of salt stress. The isolates were coded as C3A1, C8D2, and K10P4 and applied to rice plants by seed priming method. Furthermore, they were given as single innoculant or combined as a consortium compared to control, which was without the addition of endophytic bacteria, while the inoculated seed was planted on saline semisolid Fahraeus media at 4 dS m−1. The results showed that the single isolate of K10P4 endophytic bacteria increased the dry weight of rice plants, N uptake, and chlorophyll of plants in saline conditions. The combination of K10P4 isolate with C8D2 was synergistic and increased the population of endophytic bacteria in root tissue and chlorophyll content compared to the combination of C3A1 or three isolates. Meanwhile, the use of the 16S ribosomal RNA method on C3A1, C8D2, and K10P4 indentified the isolates as Ochrobactrum tritici (C3A1), Pseudomonas stutzeri (C8D2), and Pseudomonas stutzeri (K10P4).

Los estilos APA, Harvard, Vancouver, ISO, etc.

24

Zhang, Huichao, Jinpeng Xin, Huaimin Jin, Wenhao Xiang, Muqing Chen, Yang-Rong Yao y Shangfeng Yang. "TmCN@C82: Monometallic Clusterfullerene Encapsulating a Tm3+ Ion". Inorganics 11, n.º 8 (31 de julio de 2023): 323. http://dx.doi.org/10.3390/inorganics11080323.

Texto completo

Resumen

Metal cyanide clusterfullerenes (CYCFs) are formed via the encapsulation of a single metal atom and a cyanide unit inside fullerene cages, endowing them with excellent properties in various applications. In this work, we report the synthesis, isolation, and characterizations of the first cases of thulium (Tm)-based CYCFs with the popular C82 carbon cages. The structural elucidation of the two TmCN@C82 isomers was achieved via diverse analytical techniques, including mass spectrometry, Vis-NIR spectroscopy, single-crystal X-ray crystallography, and cyclic voltammetry. The crystallographic analyses unambiguously confirmed the molecular structures of the two TmCN@C82 isomers as TmCN@Cs(6)-C82 and TmCN@C2v(9)-C82. Both TmCN clusters adopt a well-established triangular configuration, with the Tm ion located on the symmetrical plane of the carbon cages. The electronic structures of both TmCN@C82 isomers adopt a Tm3+(CN)−@(C82)2− configuration, exhibiting characteristic spectral and electrochemical properties reminiscent of divalent endohedral metallofullerenes (EMFs). Intriguingly, unlike the divalent Tm2+ ion observed in the mono-metallofullerenes Tm@C2n, a higher oxidation state of Tm3+ is identified in the monometallic TmCN cluster due to bonding with the cyanide anion. This result provides valuable insight into the essential role of the non-metallic endo-units in governing the oxidation state of the metal ion and the electronic behaviors of EMFs.

Los estilos APA, Harvard, Vancouver, ISO, etc.

25

Slanina, Zdeněk, Filip Uhlík, Shyi-Long Lee, Ludwik Adamowicz, Takeshi Akasaka y Shigeru Nagase. "Computed stabilities in metallofullerene series: Al@C82, Sc@C82, Y@C82, and La@C82". International Journal of Quantum Chemistry 111, n.º 11 (31 de agosto de 2010): 2712–18. http://dx.doi.org/10.1002/qua.22808.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

26

Yang, De, Yuliang Zhao, Hua Guo, Yana Li, Poonam Tewary, Ning Zhang y Joost Oppenheim. "Nanoparticle [Gd@C82(OH)22]n induces dendritic cell maturation and promotes Th1 immune responses (131.2)". Journal of Immunology 184, n.º 1_Supplement (1 de abril de 2010): 131.2. http://dx.doi.org/10.4049/jimmunol.184.supp.131.2.

Texto completo

Resumen

Abstract [Gd@C82(OH)22]n, a fullerene-based nanoparticle of approximately 22 nm in saline solution, has been previously shown to exhibit anti-tumor effect in mouse tumor-bearing models with little toxicity, however, the underlying anti-tumor mechanism(s) remains elusive. Here we report that [Gd@C82(OH)22]n could induce phenotypic maturation of dendritic cells (DC) by stimulating DC production of cytokines including IL-12p70, upregulating DC costimulatory (CD80, CD83, and CD86) and MHC (HLA-A,B,C and HLA-DR) molecules, and switching DCs from CCL5-responsive to CCL19-responsive phenotype. In addition, [Gd@C82(OH)22]n-treated dendritic cells became functionally mature as illustrated by their capacity to activate allogeneic T cells. When mice were immunized with ovalbumin in the presence [Gd@C82(OH)22]n, [Gd@C82(OH)22]n enhanced ovalbumin-specific Th1-polarized immune responses as evidenced by predominantly increased production of IL-1β, IL-2, and IFNγ. Thus, [Gd@C82(OH)22]n nanoparticle is a potent activator of dendritic cells and Th1 immune response, which contributes to its potent anti-tumor effect.

Los estilos APA, Harvard, Vancouver, ISO, etc.

27

Zhou, Hongsheng, Po Yee Mak, Hong Mu, Duncan H. Mak, Hiroyuki Kouji, Marina Konopleva, Jorge E. Cortes, Michael Andreeff y Bing Z. Carter. "Combination of Tyrosine Kinase Inhibitor with β-Catenin/CBP Modulator C82 Reverses TKI Resistance, Eradicates Quiescent CML Stem/Progenitors Cells, and Overcomes MSC-Associated Microenvironmental Protection". Blood 124, n.º 21 (6 de diciembre de 2014): 401. http://dx.doi.org/10.1182/blood.v124.21.401.401.

Texto completo

Resumen

Abstract Bcr-Abl tyrosine kinase inhibitors (TKIs) are effective in inducing remissions and improving survival in patients with CML but do not eliminate CML leukemia stem cells (LSCs). Wnt/β-catenin pathway is established to be active in CML and essential for CML LSC, while adult HSCs do not require fully active β-catenin for maintenance. Furthermore, Wnt/β-catenin signaling pathway plays a critical role in TKI resistance and stromal-mediated microenvironmental protection for CML stem and progenitor cells. We propose that combinations of β-catenin inhibitors and TKIs represent a potentially effective therapy by targeting both CML LSCs and leukemia-mediated microenvironmental protection. C82 is a novel β-catenin/CBP modulator that via binding to CBP inhibits the interaction of β-catenin and CBP and thus disrupts Wnt/β-catenin/CBP mediated cell proliferation and self-renewal signaling. CML cell lines and primary CML-BC patient samples were treated with combinations of C82 with different TKIs, including imatinib (IM), nilotinib (NIL), dasatinib (DAS), and ponatinib (PON). Both TKI-sensitive KBM5 (IC50=0.50±0.06µM, EC50=0.32±0.01µM, 48h) and TKI-resistant KBM5-STIT315I (IC50=1.44±0.06µM, EC50=0.36±0.09µM, 48h) cells were sensitive to C82. C82-TKI combinations synergistically induced apoptosis (C82-NIL, CI=0.30±0.07 and C82-DAS, CI=0.20±0.01 in KBM5; C82-NIL, CI=0.24±0.09 and C82-DAS, CI=0.36±0.05 in KBM5-STIT315I at 48h; respectively) and inhibited cell grwoth in both cell lines. KBM5, KBM5-STIT315I and K562 were co-cultured with normal human bone marrow derived-MSCs. Western blot showed that CML/hMSCs co-cultures increased β-catenin, CD44, and survivin proteins in CML cell lines. C82-TKI combinations induced similar degrees of cell death and proliferation inhibition with or without hMSC co-cultures, indicating the combination strategy can overcome MSC-mediated microenvironmental chemoprotection in CML. Western blot analysis showed that C82 significantly inhibited CD44 and survivin expression which was further reduced by C82-TKI combinations in KBM5 and KBM5-STIT315I cells. C82-TKI combinations were evaluated in CML sample (n=6) from heavily-treated and TKI-resistant CML-BC patients. Four out of 6 sample harbored BCR-ABL kinase mutations, including T315I, E255K/V, and H396R. Mononuclear cells from the patients were stained with cell division tracking dye CFSE and then co-cultured with hMSCs. Flow cytometry was performed to identify CD34+CFSEbright and CD34+CFSEdim cells, as quiescent and proliferating population, respectively. When CML cells were treated without hMSC co-culture, C82-TKI combinations exerted stronger synergistic effects in CFSEbright quiescent cells (CI=0.21±0.06, 0.29±0.07, 0.48±0.15, or 0.26±0.03 for combination of C-82 with IM, NIL, DAS, or PON) compared with CFSEdim proliferating cells (CI=0.43±0.05, 0.43±0.17, 0.50±0.20, or 0.44±0.06 for combination of C-82 with IM, NIL, DAS, or PON; respectively). While under co-culture conditions, similar levels of synergy was observed in proliferating (CI=0.39±0.02, 0.23±0.02, 0.32±0.05, or 0.27±0.01 for combination of C-82 with IM, NIL, DAS, or PON) and quiescent cells (CI=0.23±0.02, 0.20±0.01, 0.39±0.10, or 0.20±0.04 for combination of C-82 with IM, NIL, DAS, or PON; respectively). C82-TKI combinations also synergistically induced cell death in CD34+38- CML cells (n=4) and yielded minimum effect on normal bone marrows CD34+ cells (n=3). Invivo studies are ongoing with immunodeficient NOD/SCID/IL2rγnull mice injected with CML-BC patient samples. An open-label, dose-escalation phase I/II study of PRI-724 (active metabolite of C82) for advanced myeloid malignancies (NCT01606579), including CML patients in combination with dasatinb, is enrolling patients at MD Anderson Cancer Center and other centers. Our data demonstrate that β-catenin/CBP signaling pathway plays a critical role in quiescent CML stem/progenitor cells and disruption of the β-catenin/CBP interaction with C82 could overcome MSC-mediated microenvironmental protection for not only proliferating but also quiescent stem/progenitor cells in CML. Combinations of β-catenin/CBP signaling pathway modulator C82 with TKIs represent a potentially promising strategy to tackle TKI resistance and eradicate CML stem/progenitors cells and should be further investigated in larger studies. Disclosures Kouji: PRISM Pharma Co., Ltd: Employment. Cortes:PRISM Pharma Co., Ltd: Clinicaltrial PI for NCT01606579 Other. Andreeff:PRISM Pharma Co., Ltd: Research Funding. Carter:PRISM Pharma Co., Ltd: Research Funding.

Los estilos APA, Harvard, Vancouver, ISO, etc.

28

Slanina, Zdeněk, Filip Uhlík, Takeshi Akasaka, Xing Lu y Ludwik Adamowicz. "Pr@C82 Metallofullerene: Calculated Isomeric Populations". Inorganics 11, n.º 7 (24 de julio de 2023): 313. http://dx.doi.org/10.3390/inorganics11070313.

Texto completo

Resumen

Relative equilibrium populations of the five lowest-energy isolated-pentagon-rule (IPR) isomeric structures of Pr@C82 under high-temperature fullerene synthesis conditions were calculated with the Gibbs energy terms based on molecular characteristics derived using density functional theory (DFT) treatments (B3LYP/6-31+G*∼SDD energetics and B3LYP/6-31G*∼SDD entropy). Two leading isomers were identified, major Pr@C2v;9-C82 and minor Pr@Cs;6-C82. The calculated isomeric relative equilibrium populations agreed with observations.

Los estilos APA, Harvard, Vancouver, ISO, etc.

29

Kang, Seung-gu, Guoqiang Zhou, Ping Yang, Ying Liu, Baoyun Sun, Tien Huynh, Huan Meng et al. "Molecular mechanism of pancreatic tumor metastasis inhibition by Gd@C82(OH)22 and its implication for de novo design of nanomedicine". Proceedings of the National Academy of Sciences 109, n.º 38 (4 de septiembre de 2012): 15431–36. http://dx.doi.org/10.1073/pnas.1204600109.

Texto completo

Resumen

Pancreatic adenocarcinoma is the most lethal of the solid tumors and the fourth-leading cause of cancer-related death in North America. Matrix metalloproteinases (MMPs) have long been targeted as a potential anticancer therapy because of their seminal role in angiogenesis and extracellular matrix (ECM) degradation of tumor survival and invasion. However, the inhibition specificity to MMPs and the molecular-level understanding of the inhibition mechanism remain largely unresolved. Here, we found that endohedral metallofullerenol Gd@C82(OH)22 can successfully inhibit the neoplastic activity with experiments at animal, tissue, and cellular levels. Gd@C82(OH)22 effectively blocks tumor growth in human pancreatic cancer xenografts in a nude mouse model. Enzyme activity assays also show Gd@C82(OH)22 not only suppresses the expression of MMPs but also significantly reduces their activities. We then applied large-scale molecular-dynamics simulations to illustrate the molecular mechanism by studying the Gd@C82(OH)22–MMP-9 interactions in atomic detail. Our data demonstrated that Gd@C82(OH)22 inhibits MMP-9 mainly via an exocite interaction, whereas the well-known zinc catalytic site only plays a minimal role. Steered by nonspecific electrostatic, hydrophobic, and specific hydrogen-bonding interactions, Gd@C82(OH)22 exhibits specific binding modes near the ligand-specificity loop S1′, thereby inhibiting MMP-9 activity. Both the suppression of MMP expression and specific binding mode make Gd@C82(OH)22 a potentially more effective nanomedicine for pancreatic cancer than traditional medicines, which usually target the proteolytic sites directly but fail in selective inhibition. Our findings provide insights for de novo design of nanomedicines for fatal diseases such as pancreatic cancer.

Los estilos APA, Harvard, Vancouver, ISO, etc.

30

Garrido, David Israel, Andres Orquera, Johanna Rojas y Manuel Granja. "The Mortality burden of hematological malignancies in Ecuador". Nepal Journal of Epidemiology 11, n.º 2 (29 de junio de 2021): 1040–48. http://dx.doi.org/10.3126/nje.v11i2.37057.

Texto completo

Resumen

Background: The Hematological neoplasms (HN) are a heterogeneous group of cancers that originated in the hematopoietic or lymphoid tissues. There is reduced information published regarding HN mortality in Ecuador. This study aims to present the crude and age-specific mortality rates for HN in the Ecuadorian population. Methods: We performed a cross-sectional study through the national database of defunctions published by the Ecuadorian National Institute of Statistics and Census, 2019. We used the ICD-10 codes to classify the HN. Results: During 2019, 1462 deaths were reported, 53.83% were males, 87.96% of mestizo ethnicity, and 78.32% residents in urban areas. The median age was 62 years, with an interquartile range of 34. The crude mortality rate obtained was 8.49 per 100000 inhabitants, and the higher age-specific mortality rates was 43.29 per 100000 inhabitants aged ≥ 60 years, contrasting with the 2.63 per 100000 inhabitants in people aged < 20 years. Considering each ICD-10 group, we found the following rates by 100000 inhabitants; C85 2.04, C91 1.92, C92 1.46, C90 1.11, C83 0.70, C95 0.48, C81 0.38, C84 0.16, C82 0.10, C96 0.05, C93 0.04, C86 and C94 0.02, and C88 0.01. Conclusion: In Ecuador, during 2019, approximately eight people died due to HN by 100000 inhabitants, affecting mainly people aged ≥ 60 years. The most frequent neoplasms were Non-Hodgkin lymphomas, similar to other reports globally. These results should be analyzed considering some deficiencies in the Ecuadorian health system and the national registry. Therefore, we suggest conducting more studies regarding HN.

Los estilos APA, Harvard, Vancouver, ISO, etc.

31

Wakabayashi, Tomonari, Urszula Szczepaniak, Kaito Tanaka, Satomi Saito, Keisuke Fukumoto, Riku Ohnishi, Kazunori Ozaki et al. "Phosphorescence of Hydrogen-Capped Linear Polyyne Molecules C8H2, C10H2 and C12H2 in Solid Hexane Matrices at 20 K". Photochem 2, n.º 1 (28 de febrero de 2022): 181–201. http://dx.doi.org/10.3390/photochem2010014.

Texto completo

Resumen

Laser-ablated polyyne molecules, H(C≡C)nH, were separated by size in solutions and co-condensed with excess hexane molecules at a cryogenic temperature of 20 K in a vacuum system. The solid matrix samples containing C8H2, C10H2, and C12H2 molecules were irradiated with UV laser pulses and the phosphorescence 0–0 band was observed at 532, 605, and 659 nm, respectively. Vibrational progression was observed for the symmetric stretching mode of the carbon chain in the ground state with increments of ~2190 cm−1 for C8H2, ~2120 cm−1 for C10H2, and ~2090 cm−1 for C12H2. Temporal decay analysis of the phosphorescence intensity revealed the lifetimes of the triplet state as ~30 ms for C8H2, ~8 ms for C10H2, and ~4 ms for C12H2. The phosphorescence excitation spectrum reproduces UV absorption spectra in the hexane solution and in the gas phase at ambient temperature, although the excitation energy was redshifted. The symmetry-forbidden vibronic transitions were observed for C10H2 by lower excitation energies of 25,500–31,000 cm−1 (320–390 nm). Detailed phosphorescence excitation patterns are discussed along the interaction of the polyyne molecule and solvent molecules of hexane.

Los estilos APA, Harvard, Vancouver, ISO, etc.

32

Zhang, Guo Lin, Jian Min Zhang y Hong Kuan Yuan. "Density-Functional Theory Study of Ce@C₈₂". Advanced Materials Research 482-484 (febrero de 2012): 2577–81. http://dx.doi.org/10.4028/www.scientific.net/amr.482-484.2577.

Texto completo

Resumen

The magnetic ground state structure of the metallofullerene Ce@C82 is confirmed by the density functional calculations. The results show that the Ce atom is located inside the C82 cage with site at the C2 symmetry axis. The effective magnetic moment of Ce@C82 is increased relative to the value of a free Ce3+ ion. The reason is that there is hybridization between unoccupied Ce-4f states and carbon-π states, which result in a general ferromagnetic coupling of the Ce-4f spin with the remaining unpaired spin in the hybridized molecular orbital.

Los estilos APA, Harvard, Vancouver, ISO, etc.

33

Miyazaki, Takafumi, Ryohei Sumii, Hisashi Umemoto, Haruya Okimoto, Yasuhiro Ito, Toshiki Sugai, Hisanori Shinohara, Takeyuki Zaima, Hajime Yagi y Shojun Hino. "Ultraviolet photoelectron spectra of Er2@C82 (I), Er2@C82 (III), Er2C2@C82 (I) and Er2C2@C82 (III)". Chemical Physics 397 (marzo de 2012): 87–91. http://dx.doi.org/10.1016/j.chemphys.2012.01.007.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

34

Mikheev, Ivan V., Madina M. Sozarukova, Dmitry Yu Izmailov, Ivan E. Kareev, Elena V. Proskurnina y Mikhail A. Proskurnin. "Antioxidant Potential of Aqueous Dispersions of Fullerenes C60, C70, and Gd@C82". International Journal of Molecular Sciences 22, n.º 11 (29 de mayo de 2021): 5838. http://dx.doi.org/10.3390/ijms22115838.

Texto completo

Resumen

The antioxidant potential (capacity and activity) of aqueous fullerene dispersions (AFD) of non-functionalized C60, C70, and Gd@C82 endofullerene (in micromolar concentration range) was estimated based on chemiluminescence measurements of the model of luminol and generation of organic radicals by 2,2′-azobis(2-amidinopropane) dihydrochloride (ABAP). The antioxidant capacity was estimated by the TRAP method, from the concentration of half-suppression, and from the suppression area in the initial period. All three approaches agree and show that the antioxidant capacity of AFDs increased in the order Gd@C82 < C70 < C60. Mathematical modeling of the long-term kinetics data was used for antioxidant activity estimation. The effect of C60 and C70 is found to be quenching of the excited product of luminol with ABAP-generated radical and not an actual antioxidant effect; quenching constants differ insignificantly. Apart from quenching with a similar constant, the AFD of Gd@C82 exhibits actual antioxidant action. The antioxidant activity in Gd@C82 is 300-fold higher than quenching constants.

Los estilos APA, Harvard, Vancouver, ISO, etc.

35

Kageyama, Takuya, Shunsuke Uneme, Masayoshi Takase, Kotohiro Nomura y Tohru Nishinaga. "Diradical Character of Benzo- and Naphtho-Annelated Thiophene–Pyrrole Mixed Oligomer Dications". Australian Journal of Chemistry 67, n.º 5 (2014): 722. http://dx.doi.org/10.1071/ch13522.

Texto completo

Resumen

Benzo- and naphtho-annelated thiophene–pyrrole mixed octamers Bz8TP-Cm and Np8TP-Cm, comprising benzo- or naphthodithiophene and two dithienylpyrrole units, were synthesised. Density functional theory (DFT) calculations based on B3LYP/6–31G(d) with the broken symmetry method predicted that dications Bz8TP-C12+ and Np8TP-C12+ have stronger diradical character than previously investigated non-annelated thiophene–pyrrole mixed octamer 8TP-C12+ (R1 = R2 = H). Compounds Bz8TP-C8 and Np8TP-C8 showed a one-step, two-electron oxidation process based on cyclic voltammetry analysis. Reaction with SbCl5 involved a two-electron oxidation. Bz8TP-C82+ and Np8TP-C82+ displayed similar absorption spectra to that of nonamer 9TP-C122+ (R1 = R2 = H), rather than that of octamer 8TP-C122+, indicating the stronger diradical characters of octamers Bz8TP-C82+ and Np8TP-C82+, as enhanced by benzo- and naphtho-annelation, and were comparable with that of 9TP-C122+. Time-dependent-DFT calculations supported the conclusion obtained from the experimental results.

Los estilos APA, Harvard, Vancouver, ISO, etc.

36

Wang, Jingjing, Dongjie Chen, Fang Wei, Junhua Deng, Jia Su, Xiangmei Lin y Shaoqiang Wu. "Generation of Stable Cell Lines Expressing Akabane Virus N Protein and Insight into Its Function in Viral Replication". Pathogens 12, n.º 8 (18 de agosto de 2023): 1058. http://dx.doi.org/10.3390/pathogens12081058.

Texto completo

Resumen

Akabane virus (AKAV) is a world wide epidemic arbovirus belonging to the Bunyavirales order that predominantly infects livestock and causes severe congenital malformations. The nucleocapsid (N) protein of AKAV possesses multiple important functions in the virus life cycle, and it is an ideal choice for AKAV detection. In this study, we successfully constructed two stable BHK-21 cell lines (C8H2 and F7E5) that constitutively express the AKAV N protein using a lentivirus system combined with puromycin selection. RT-PCR analysis confirmed that the AKAV N gene was integrated into the BHK-21 cell genome and consistently transcribed. Indirect immunofluorescence (IFA) and Western blot (WB) assays proved that both C8H2 and F7E5 cells could react with the AKAV N protein mAb specifically, indicating potential applications in AKAV detection. Furthermore, we analyzed the growth kinetics of AKAV in the C8H2 and F7E5 cell lines and observed temporary inhibition of viral replication at 12, 24 and 36 h postinfection (hpi) compared to BHK-21 cells. Subsequent investigations suggested that the reduced viral replication was linked to the down-regulation of the viral mRNAs (Gc and RdRp). In summary, we have established materials for detecting AKAV and gained new insights into the function of the AKAV N protein.

Los estilos APA, Harvard, Vancouver, ISO, etc.

37

Anzuela, Elena, María A. Garralda, Ricardo Hernández, Lourdes Ibarlucea, Elena Pinilla y M. Angeles Monge. "Rhodium(I) complexes with unsymmetric aliphatic diamines. Crystal structure of [Rh(C8H12)(C7N2H16)][RhCl2(C8H12)] and [Rh(C8H12)(C7N2H16)]ClO4". Inorganica Chimica Acta 185, n.º 2 (julio de 1991): 211–19. http://dx.doi.org/10.1016/s0020-1693(00)85446-8.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

38

Harrold, Karen. "Development of a nurse-led service for patients receiving oral capecitabine". Cancer Nursing Practice 1, n.º 8 (octubre de 2002): 19–24. http://dx.doi.org/10.7748/cnp2002.10.1.8.19.c8102.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

39

Neame, Bryony. "The chic delivery of anti-cancer drugs". Cancer Nursing Practice 7, n.º 4 (mayo de 2008): 18–20. http://dx.doi.org/10.7748/cnp2008.05.7.4.18.c8162.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

40

Holmes, Lynn. "Identifying side effects of pelvic radiotherapy". Cancer Nursing Practice 9, n.º 10 (2 de diciembre de 2010): 12–18. http://dx.doi.org/10.7748/cnp2010.12.9.10.12.c8124.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

41

Jones, Helen, Anne Croudass y Sophie Lewis. "Facilitating the link between research and clinical practice". Cancer Nursing Practice 9, n.º 10 (2 de diciembre de 2010): 23–26. http://dx.doi.org/10.7748/cnp2010.12.9.10.23.c8125.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

42

Turner, Bruce y Lawrence Drudge-Coates. "Prostate cancer: risk factors, diagnosis and management". Cancer Nursing Practice 9, n.º 10 (2 de diciembre de 2010): 29–36. http://dx.doi.org/10.7748/cnp2010.12.9.10.29.c8126.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

43

MacGregor, Janet y Tony Gray. "Is a CCN degree the only way to develop children’s nursing in the community?" Paediatric Care 14, n.º 5 (junio de 2002): 21–25. http://dx.doi.org/10.7748/paed2002.06.14.5.21.c802.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

44

Hancock, Julia. "Preceptorship on a Neonatal Intensive Care Unit: Evaluating effectiveness". Paediatric Care 14, n.º 6 (julio de 2002): 33–37. http://dx.doi.org/10.7748/paed2002.07.14.6.33.c810.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

45

Price, Sue. "The recruitment and retention of children’s nurses". Paediatric Care 14, n.º 6 (julio de 2002): 39–43. http://dx.doi.org/10.7748/paed2002.07.14.6.39.c811.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

46

Simms, Susan, Nicki Hewitt y Vevers June. "Sibling support in childhood cancer". Paediatric Care 14, n.º 7 (septiembre de 2002): 20–22. http://dx.doi.org/10.7748/paed2002.09.14.7.20.c813.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

47

Ennis, Linda. "Renal anaemia in children: the role of the specialist nurse". Paediatric Care 14, n.º 7 (septiembre de 2002): 24–27. http://dx.doi.org/10.7748/paed2002.09.14.7.24.c814.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

48

Flatman, Dorothy. "Consulting children: are we listening?" Paediatric Care 14, n.º 7 (septiembre de 2002): 28–31. http://dx.doi.org/10.7748/paed2002.09.14.7.28.c815.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

49

Pantrini, Shiela A. "A window of opportunity: preventing Shaken Baby Syndrome in A&E". Paediatric Care 14, n.º 7 (septiembre de 2002): 32–34. http://dx.doi.org/10.7748/paed2002.09.14.7.32.c816.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

50

Clarke, Dave, Jane Davies y Peter McNee. "The case for a children’s nursing skills laboratory". Paediatric Care 14, n.º 7 (septiembre de 2002): 36–39. http://dx.doi.org/10.7748/paed2002.09.14.7.36.c817.

Texto completo

Los estilos APA, Harvard, Vancouver, ISO, etc.

Artículos de revistas sobre el tema "C812"

Crea una cita precisa en los estilos APA, MLA, Chicago, Harvard y otros