Tesis sobre el tema "Biosensing platform"
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Ravindran, Ramasamy. "An electronic biosensing platform". Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/44774.
Texto completoShi, Jingxing. "Integrated photonic crystals platform for biosensing". Thesis, University of Southampton, 2018. https://eprints.soton.ac.uk/423474/.
Texto completoKing, Branden Joel. "Tapered Optical Fiber Platform for Biosensing Applications". University of Dayton / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1398708775.
Texto completoPerelló, Roig Rafel. "Fully integrated CMOS-MEMS resonators as a biosensing platform". Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2021. http://hdl.handle.net/10803/673937.
Texto completo[cat] La diversificació de funcionalitats en un mateix sistema integrat intrínsec a l’aproximació “More than Moore” ha evidenciat un interès creixent en els dispositius basats en ressonadors MEMS per aplicacions tipus “System-on-Chip” gràcies a la seva capacitat de miniaturització, a la implementació directa d’esquemes de lectura elèctrics i a la seva possible integració amb les tecnologies actuals de fabricació de circuits integrats. Aquests avantatges els fan candidats idonis pel desenvolupament d’aplicacions de bio-sensat en l’àmbit de la química i de la biologia donada la seva portabilitat, elevat rendiment, dimensions reduïdes i temps mínim de processat. Aquest treball de tesi es focalitza en l’anàlisi, disseny i desenvolupament de ressonadors MEMS orientats i optimitzats per a la detecció de COVs i pel sensat calorimètric, integrats monolíticament amb circuits CMOS de lectura mitjançant l’ús d’una tecnologia comercial CMOS de 0.35-μm i d’una estratègia de fabricació CMOS-MEMS. Els ressonadors MEMS desenvolupats poden operar en mode oscil·lador, gràcies a la implementació d’un esquema elèctric amb actuació electrostàtica i lectura capacitiva, que permet la seva connexió directa a un circuit amplificador específic obtenint-se així un senyal de sortida quasi digital. Els dispositius MEMS s’han mecanitzat emprant les capes disponibles de la tecnologia CMOS mitjançant una aproximació intra-CMOS per la seva fabricació. Aquesta requereix d’una etapa posterior de gravat humit per a l’alliberació de les parts mecàniques mòbils dels ressonadors basada en l’eliminació de l’òxid de sacrifici tot mantenint-se protegit la resta del circuit integrat gràcies a la capa de passivació. En aquest treball es presenten un gran nombre d’estudis analítics i simulacions FEM que, juntament amb la caracterització experimental tant en llaç obert com en llaç tancat dels diferents dispositius CMOS-MEMS fabricats, suposen una important font d’informació per a l’optimització dels dissenys. S’han dissenyat ressonadors tipus plataforma amb quatre ancoratges que operen dins el rang dels MHz i seguidament, s’han avaluat per la seva operació com a sensors de gas gravimètrics mitjançant una funcionalització superficial específica (ja sigui a través d’immersió en dissolució o bé per deposició d’un polímer emprant impressió de tinta) amb els que s’ha obtingut una resolució en massa per unitat de superfície de 213 pg·cm-2·Hz-1 així com una desviació d’Allan inferior als 0.5 ppm. Addicionalment s’ha analitzat la tolerància que presenten varies topologies de suport front a pertorbacions ambientals com la temperatura, la humitat i el flux d’un gas constatant que les estructures tipus “U” minimitzen aquests efectes fins 20 vegades. Mitjançant la deposició del polímer P4V sobre els sistemes fabricats, s’ha demostrat la capacitat de detectar acetona amb una resolució de 20 ppb el que capacita per la seva potencial aplicació en el diagnosi clínic de pacients diabètics de forma no invasiva per mitjà de l’exhalat. En el cas dels sensors calorimètrics, s’han dissenyat ressonadors tipus pont amb dos ancoratges que operen dins el rang dels MHz i funcionen com a sensors de temperatura de molt alta sensibilitat assolint un valors de fins a -7900 ppm·ºC-1 que, juntament amb una desviació Allan de l’oscil·lador inferior a ppm, s’assoleix una excel·lent resolució tèrmica de 300 μK. En aquesta línia d’actuació, s’ha desenvolupat tot un procés de co-integració dels ressonadors juntament amb una plataforma de microfluídica basada en PDMS amb el que s’obté un sistema tipus “Lab-on-Chip” compatible amb circuits integrats i que permet dirigir un fluid d’interès per a la seva interacció sobre un elèctrode CMOS. Es presenta un procés de planarització 2D, que permet incrementar la superfície efectiva del xip, juntament amb la utilització de la tècnica “wire bonding” estàndard per a la connexió elèctrica amb l’encapsulat. Els resultats experimentals confirmen l’operació del sistema com a micro-calorímetre obtenint una eficiència tèrmica de la mostra cap al ressonador del 20%, que esdevé en una resolució d’energia i calor de 150 pJ i 630 nW, respectivament.
[spa] La diversificación de funcionalidades en un mismo sistema integrado intrínseca a la aproximación “More than Moore” ha evidenciado un interés creciente en los dispositivos basados en resonadores MEMS para aplicaciones tipo “System-on-Chip” gracias a su capacidad de miniaturización, a la implementación directa de esquemas de lectura eléctricos y a su posible integración con las tecnologías actuales de fabricación de circuitos integrados. Estas ventajas los hacen candidatos idóneos para el desarrollo de aplicaciones de bio-sensado en el ámbito de la química y de la biología dada su portabilidad, elevado rendimiento, tamaño reducido y tiempo mínimo de procesado. Este trabajo de tesis se focaliza en el análisis, diseño y desarrollo de resonadores MEMS, orientados y optimizados para la detección de COVs y para sensado calorimétrico, integrados monolíticamente con circuitos CMOS de lectura mediante el uso de una tecnología comercial CMOS de 0.35-μm y de una estrategia de fabricación CMOS-MEMS. Los resonadores MEMS desarrollados pueden operar como oscilador, gracias a la implementación de un esquema eléctrico con actuación electrostática y lectura capacitiva, que permite su conexión directa a un circuito amplificador específico obteniéndose así una señal de salida casi digital. Los dispositivos MEMS se han mecanizado utilizando las capas disponibles de la tecnología CMOS mediante una aproximación intra-CMOS para su fabricación. Esta requiere de una etapa posterior de gravado húmedo para la liberación de las partes mecánicas móviles de los resonadores basada en la eliminación del óxido de sacrificio manteniéndose protegido el resto del circuito integrado gracias a una capa de pasivación. En este trabajo se presentan un gran número de estudios analíticos y simulaciones FEM que, junto con la caracterización experimental tanto en lazo abierto como lazo cerrado de los diferentes dispositivos CMOSMEMS fabricados, suponen una importante fuente de información para la optimización de los diseños. Se han diseñado resonadores tipo plataforma con cuatro anclajes que operan en el rango de los MHz y seguidamente, se han evaluado para su operación como sensores de gas gravimétricos mediante una funcionalización superficial específica (ya sea a través de inmersión en disolución o bien por deposición de un polímero usando impresión de tinta) con los que se ha obtenido una resolución en masa por unidad de superficie de 213 pg·cm-2·Hz-1 así como una desviación Allan inferior a los 0.5 ppm. Adicionalmente se ha analizado la tolerancia que presentan varias topologías de anclaje frente a perturbaciones ambientales como la temperatura, la humedad y el flujo de un gas constatándose que las estructuras tipo “U” minimizan estos efectos hasta 20 veces. Mediante la deposición del polímero P4V sobre los sistemas fabricados, se ha demostrado la capacidad de detectar acetona con una resolución de 20 ppb lo que capacita para su potencial aplicación en el diagnóstico clínico de pacientes diabéticos de forma no invasiva a través del exhalado. En el caso de los sensores calorimétricos, se han diseñado resonadores tipo puente de doble anclaje que operan en el rango de los MHz y funcionan como sensores de temperatura de muy alta sensibilidad alcanzando valores de hasta -7900 ppm·ºC-1 que, junto con una desviación Allan del oscilador inferior a ppm, se consigue una excelente resolución térmica de hasta 300 μK. En esta línea de actuación, se ha desarrollado todo un proceso de co-integración de los resonadores junto con una plataforma de microfluídica basada en PDMS con lo que se obtiene un sistema tipo “Lab-on-Chip” compatible con circuitos integrados y que permite dirigir un fluido de interés para su interacción sobre un electrodo CMOS. Se presenta un proceso de planarización 2D, que permite incrementar la superficie efectiva del chip, junto con la utilización de la técnica “wire bonding” estándar para la conexión eléctrica con el encapsulado. Los resultados experimentales confirman la operación del sistema como micro-calorímetro obteniendo una eficiencia térmica de la muestra hacia el resonador del 20%, lo que supone una resolución de energía y calor de 150 pJ y 630 nW, respectivamente.
Sogne, E. "NOVEL PLATFORM FOR BIOSENSING APPLICATION BASED ON CLUSTER-ASSEMBLED MATERIALS". Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/265569.
Texto completoBhatnagar, Purva. "Multi-Frequency and Multi-Sensor Impedance Sensing Platform for Biosensing Applications". University of Cincinnati / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1543999395772179.
Texto completoDaaboul, George. "Interferometric biosensing platform for multiplexed digital detection of viral pathogens and biomarkers". Thesis, Boston University, 2013. https://hdl.handle.net/2144/10976.
Texto completoLabel-free optical biosensors have been established as proven tools for monitoring specific biomolecular interactions. However, compact and robust embodiments of such instruments have yet to be introduced in order to provide sensitive, quantitative, and high-throughput biosensing for low-cost research and clinical applications. Here we present the interferometric reflectance-imaging sensor (IRIS). IRIS allows sensitive label free analysis using an inexpensive and durable multi-color LED illumination source on a silicon based surface. IRIS monitors biomolecular interaction through measurement of biomass addition to the sensor's surface. We demonstrate the capability of this system to dynamically monitor antigen-antibody interactions with a noise floor of 5.2 pg/mm^2 and DNA single mismatch detection under isothermal melting conditions in an array format. Ensemble detection of binding events using IRIS did not provide the sensitivity needed for detection of infectious disease and biomarkers at clinically relevant concentrations. Therefore, a new approach was adapted to the IRIS platform that allowed the detection and identification of individual nanoparticles on the sensor's surface. The new detection method was te1med single-particle IRIS (SP-IRIS). We developed two detection modalities for SP-IRIS. The first modality is when the target is a nanoparticle such as a virus. We verified that SP-IRIS can accurately detect and size individual viral particles. Then we demonstrated that single nanoparticle counting and sizing methodology on SP-IRIS leads to a specific and sensitive virus sensor that can be multiplexed. Finally, we developed an assay for the detection of Ebola and Marburg. A detection limit of 3 x 10^3 PFU/ml was demonstrated for vesicular stomatitis virus (VSV) pseudotyped with Ebola or Marburg virus glycoprotein. We have demonstrated that virus detection can be done in human whole blood directly without the need for sample preparation. The second modality of SP-IRIS we developed was single molecule counting of biomarkers utilizing a sandwich assay with detection probes labeled with gold nanoparticles. We demonstrated the use of single molecule counting in a nucleic acid assay for melanoma biomarker detection. We showed that a single molecule counting assay can lead to detection limits in the attomolar range. The improved sensitivity of IRIS utilizing single nanoparticle detection holds promise for a simple and low-cost technology for rapid virus detection and multiplexed molecular screening for clinical applications.
Kendall, James Kenneth Roger. "Development of a tethered biomembrane biosensing platform for the incorporation of ion channels". Thesis, University of Leeds, 2011. http://etheses.whiterose.ac.uk/2589/.
Texto completoCastro, Arias Juan Manuel. "Towards a Plasmonic and Electrochemical Biosensor Integrated in a Microfluidic Platform". Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS020/document.
Texto completoDuring my thesis, I worked on the development of a specific fabrication process able to produce a device that combines two different biodetection techniques, plasmonic response based on Localized Surface Plasmon Resonance (LSPR) and electrochemical response. Methods and results that are presented in this manuscript were defined to converge towards a unique fluidic device combining these two different sensing approaches. This device integrates interdigitated array of MIM nanocavities. In order to find the easier working configuration allowing the excitation of plasmonic resonances, their geometry has been optimized through electromagnetic simulations. The fabrication of these dual devices has been optimized based on Soft-UV NIL and, finally, optical characterization of these nanocavities has been successfully compared with theoretical simulations. In parallel to this challenging goal, simpler fluidic electrochemical devices that integrate conventional microelectrodes have also been developed. The goal was first to develop an innovative chemistry for the couple biotin/streptavidin and secondly to learn how fluidic parameters can affect the capture efficiency of molecules. This manuscript ends with a discussion on the role of the fluidic parameters on the biodetection efficiency based on the theory of Squires
Kumarswami, Neelam. "Development of a multiplexing biosensor platform using SERS particle immunoassay technology". Thesis, University of Bedfordshire, 2014. http://hdl.handle.net/10547/321094.
Texto completoDoherty, Brenda [Verfasser], Markus A. [Gutachter] Schmidt, Rachel [Gutachter] Grange y Isabelle Philippa [Gutachter] Staude. "Plasmonic microstructured optical fibres : an efficient platform towards biosensing / Brenda Doherty ; Gutachter: Markus A. Schmidt, Rachel Grange, Isabelle Philippa Staude". Jena : Friedrich-Schiller-Universität Jena, 2020. http://d-nb.info/121099853X/34.
Texto completoWang, Wenxing. "Development of microcantilever biosensing platforms". Thesis, Heriot-Watt University, 2013. http://hdl.handle.net/10399/2722.
Texto completoTUOHETI, ABUDUWAILI. "Smart Embedded Systems for Biomedical Applications". Doctoral thesis, Politecnico di Torino, 2019. http://hdl.handle.net/11583/2742529.
Texto completoOakland, Chloe. "Lanthanide upconversion nanophosphors as platforms for luminescent biosensing applications". Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/lanthanide-upconversion-nanophosphors-as-platforms-for-luminescent-biosensing-applications(5a40bf86-83bb-455e-93c9-7ac488955c45).html.
Texto completoSena, Torralba Amadeo. "Development and application of innovative point-of-care biosensing platforms". Doctoral thesis, Universitat Autònoma de Barcelona, 2020. http://hdl.handle.net/10803/670851.
Texto completoEl objetivo de esta tesis ha sido el desarrollo y la aplicación de plataformas innovadoras de biosensado en el punto de atención. La tesis se divide en cinco capítulos seguidos de una sección de conclusiones generales. El Capítulo 1 comienza con una introducción general a los conceptos de biosensores y biosensado en el punto de atención. Luego, se enfoca en una de las pruebas de punto de atención más exitosas: el ensayo de flujo lateral (LFA). Los aspectos clave del ensayo, como los componentes y reactivos, los procedimientos de fabricación y operación están cubiertos en profundidad. El capítulo continúa con una revisión de los desafíos actuales de LFA ha enfrentado y las mejoras más relevantes reportadas en los últimos años. El Capítulo 2 describe brevemente los objetivos que motivaron este trabajo. El Capítulo 3 presenta una nueva plataforma de detección (PEB) que combina las características clave de un ensayo de flujo lateral, la prueba de punto de atención más utilizada, con las capacidades de tratamiento de muestras de la electroforesis. En particular, se demuestra la capacidad de PEB para separar diferentes tipos de partículas y detectar anticuerpos IgG humanos en muestras de sangre no tratadas. Finalmente, para hacer que la plataforma sea aplicable en el punto de atención, PEB se combina con un teléfono inteligente que controla la electroforesis y lee la señal óptica generada. El Capítulo 4 explica una estrategia simple y de bajo costo para mejorar el rendimiento analítico de los LFA. Mediante el uso de barreras de cera solubles, las nanopartículas se acumulan temporalmente en la parte superior de la línea de detección (TL). Este paso extendido de incubación interna promueve la formación del inmunocomplejo, generando una mejora de sensibilidad y de señal en comparación con la detección convencional de LFA para IgG humana (H-IgG). El Capítulo 5 presenta una plataforma de detección en el punto de atención que consiste en un microtubo y dos piezas de fibra de vidrio. El principio de detección se basa en la transferencia de energía de resonancia de Förster utilizando nanoclusters de oro como indicador de señal y nanopartículas de oro conjugadas con anticuerpos como un desactivador. La plataforma ha sido validada para la detección de Escherichia coli O157: H7 en agua de río y del grifo, demostrando una elevada sensibilidad.
The objective of this thesis has been the development and application of innovative biosensing platforms at the point of care. The thesis is divided into five chapters followed by a section of general conclusions. Chapter 1 begins with a general introduction to the concepts of biosensors and point-of-care biosensing. Then, it focuses on one of the most successful point-of-care tests: the lateral flow test (LFA). Key aspects of the assay, such as components and reagents, manufacturing and operating procedures are covered in depth. The chapter continues with a review of the current challenges LFA has faced and the most relevant improvements reported in recent years. Chapter 2 briefly describes the objectives that motivated this work. Chapter 3 introduces a new detection platform (PEB) that combines the key features of a lateral flow assay, the most widely used point-of-care test, with the capabilities of electrophoresis sample treatment. In particular, the ability of PEB to separate different types of particles and detect human IgG antibodies in untreated blood samples is demonstrated. Finally, to make the platform applicable at the point of care, PEB is combined with a smartphone that controls the electrophoresis and reads the generated optical signal. Chapter 4 explains a simple, low-cost strategy to improve the analytical performance of LFAs. By using soluble wax barriers, nanoparticles temporarily accumulate at the top of the detection line (TL). This extended internal incubation step promotes immunocomplex formation, generating improved sensitivity and signal compared to conventional LFA detection for human IgG (H-IgG). Chapter 5 presents a point-of-care detection platform consisting of a microtube and two pieces of fiberglass. The detection principle is based on Förster resonance energy transfer using gold nanoclusters as a signal indicator and antibody-conjugated gold nanoparticles as a quencher. The platform has been validated for the detection of Escherichia coli O157: H7 in river and tap water, demonstrating high sensitivity.
Soh, Jun Hui. "Development of novel biosensing and diagnostic platforms using nanoparticle complexes". Thesis, Imperial College London, 2015. http://hdl.handle.net/10044/1/43845.
Texto completoMedina, Sánchez Mariana. "Improved biosensing applications using lab-on-a-chip and other platforms". Doctoral thesis, Universitat Autònoma de Barcelona, 2013. http://hdl.handle.net/10803/129331.
Texto completoSimple and miniaturized micro / nanofluidic platforms are especially interesting due to their advantages like the reduction of sample and reagent volumes, the decrease of the analysis time, the possibility of portability and the integration of conventional analytical techniques. Furthermore it is important to point out the role that nanomaterials can play in terms of enhancing electrochemical properties after being integrated into the microfluidic platform or even in the electrode, where the detection event will be performed. Combined together, nanotechnology, electrochemistry and microfluidics could provide a really powerful biosensor platform, thus in the present Thesis different microfluidic platforms with integrated electrodes as transducers in biosensing applications were evaluated. General aspects and experimental results are exposed, starting from a General Introduction that describes various aspects related with the use of nanomaterials and lab-on-a-chip technologies as a promising synergy for a wide range of applications. The electrochemical detection of proteins (ex. Apolipoprotein-E, ApoE) by using CdS or CdSe@ZnS Quantum Dots (QDs) as labels has been one of the main objectives of this Thesis. The immunocomplex was performed by using tosylactivated magnetic beads as preconcentration platform into the same microfluidic system. Due to the need to achieve a lower limit of detection of the immunoassays, different strategies for electrochemical signal enhancing are proposed. The first one is the use of a magnetic field to immobilize magnetic beads in a controllable way into a microfluidic channel in order to obtain a stable magnetic plug where the immunoassay is performed. The second strategy is the use of a home-made recycling system. In this part, the increasing signal of QDs is demonstrated by using an external peristaltic pump connected to a microfluidic chip forming a loop system. After this demonstration, a micro-peristaltic pump with integrated valves is also proposed. All the fabrication steps have been optimized and the software for sequential control of the valves also has been developed. Finally, bismuth is used as it is a well-known material that agglomerates with heavy metals. We took advantages of this property for improving the electrochemical signal of QDs, due to the cadmium content that QDs have in their core. Optimization of the bismuth concentration has been done in order to achieve the highest signal. This detection has been performed in batch system as well as in microfluidic mode. In addition, another novel platform for electrochemical determination of a pesticide (atrazine) based on magneto-immunoassay using boron-doped diamond (BDD) electrode is presented. BDD electrode has been modified by electroreduction of potassium tetrachloroplatinate (K2PtCl4) in order to grow platinum nanoparticles (Pt-NPs) onto the electrode surface. The immunoassay was based on a direct competitive assay using horseradish peroxidase (HRP) as enzymatic label and magnetic microparticles as preconcentration platform. A flexible organic double gate Bio-Field Effect Transistor (Bio-FET) developed by inkjet technology onto a flexible substrate is also presented. This kind of organic transducers has important advantages for biosensors in terms of fabrication cost and biocompatibility as well as their integration into microchannels. To demonstrate the applicability of this device in the biological field, its functionalization with a capture antibody, in order to detect a model protein in a label-free mode was performed. The device fabrication, its structure, materials composition optimization, electrical characteristics and other functionalities are also discussed. Finally, the general conclusions are exposed including some opinions / recommendations for further continuation of the research in the field.
Meneghello, Anna. "Surface plasmon resonance based platforms for clinical and environmental biosensing applications". Doctoral thesis, Università degli studi di Padova, 2016. http://hdl.handle.net/11577/3424512.
Texto completoIl mio lavoro di Tesi di Dottorato, sviluppato presso i laboratori Veneto Nanotech (Nanofab a Marghera, LaNN a Padova ed ECSIN a Rovigo), ha avuto come obiettivo l’utilizzo della tecnologia di risonanza plasmonica di superficie (SPR – Surface Plasmon Resonance) per lo sviluppo di piattaforme biosensoristiche per applicazioni clinica ed ambientali. In particolare, durante il lavoro di Dottorato sono state messe a punto due piattaforme SPR: la prima piattaforma utilizza sonde oligonucleotidiche a DNA per l'individuazione di mutazioni causanti fibrosi cistica (CF) mentre la seconda utilizza anticorpi per il rilevamento di cellule di Legionella pneumophila. Entrambi i sensori sono basati sulla stessa strategia di rilevamento, ovvero l’utilizzo di una metodologia Grating Coupled – Surface Plasmon Resonance (GC-SPR) progettata utilizzando una configurazione conica di illuminazione ad azimut rotato per la rilevazione diretta – senza passaggi di marcatura, label-free – dell’analita in esame. Per quanto riguarda la piattaforma a DNA per la fibrosi cistica, si è sviluppata una strategia per l'individuazione di alcune delle mutazioni più frequenti responsabili CF tra la popolazione italiana. Per la rilevazione di tali mutazioni le superfici di analisi utilizzate sono grigliati sinusoidali, e la rilevazione specifica delle sequenze di interesse si ottiene attraverso l'utilizzo di oligonucleotidi allele-specifici (ASO – allele specific oligonucleotide) con struttura ad hairpin, disegnati per la discriminazione di un singolo nucleotide. I substrati plasmonici sono stati utilizzati per testare campioni wild-type ed eterozigoti (wt/mut) per le mutazioni in esame, amplificati tramite PCR a partire da campioni clinici. Le condizioni di ibridazione sono state ottimizzate per ottenere il rapporto di discriminazione (DR – discrimination ratio) massimo tra campioni wild-type ed eterozigoti. I segnali SPR ottenuti ibridando campioni wild-type e campioni eterozigoti hanno mostrato DR in grado di identificare univocamente i genotipi corretti, come confermato da esperimenti di fluorescenza in microarray eseguiti in parallelo. Inoltre la genotipizzazione ottenuta tramite SPR non è stata inficiata in campioni contenenti DNA interferente, consentendo quindi di utilizzare la piattaforma per la discriminazione in parallelo dei diversi alleli, e la possibilità futura di scalare il sistema con un approccio di high throughput screening. Per quanto riguarda la piattaforma ad anticorpi per la rilevazione di Legionella pneumophila, la medesima strategia basata su GC-SPR è stata messa a punto per ottenere una rilevazione rapida e sensibile di tale patogeno. Il limite legale di L. pneumophila in ambienti ospedalieri ad alto rischio in Italia è di 102 UFC/L (unità formanti colonia) e la metodologia di riferimento per la sua identificazione è una tecnica di coltura microbiologica che richiede tempi di attesa fino a 7 giorni. Partendo da tali considerazioni un sistema GC-SPR altamente sensibile è stato sviluppato ed applicato per la rivelazione di L. pneumophila: la rivelazione è stata accuratamente impostata ed ottimizzata con un ceppo standard del battere, prima attraverso l'utilizzo di superfici d’oro non nanostrutturate (flat) opportunamente funzionalizzate ed analizzate tramite fluorescenza, e successivamente attraverso reticoli sinusoidali (grating) d’oro analizzati tramite elissometria GC-SPR. Attraverso la metodologia GC-SPR ad azimut rotato è stato possibile rilevare fino a 10 UFC, mentre con l’analisi in fluorescenza non è stato possibile identificare quantitativi di battere inferiori a 104 UFC. Risultati positivi sono stati ottenuti anche incubando campioni di L. pneumophila isolati direttamente dall’ambiente ospedaliero. Questa piattaforma di rilevazione potrà essere implementata come prototipo in cui campioni di acqua e aria potranno venir sequenzialmente concentrati, iniettati in un sistema di microfluidica, ed incubati sulla superficie del sensore SPR per l'analisi, obiettivi questi del progetto POSEIDON (Horizon2020) attualmente in corso. La particolare metodologia GC-SPR ad azimut rotato applicata in questo lavoro di Tesi si è dimostrata essere una strategia accurata e altamente sensibile - con possibilità di multiplexing - per la rilevazione di diversi tipi di biomolecole, a partire da frammenti di DNA fino ad intere cellule batteriche.
Yavas, Ozlem. "On-chip biosensing platforms based on gold and silicon optical nano-resonators". Doctoral thesis, Universitat Politècnica de Catalunya, 2019. http://hdl.handle.net/10803/667307.
Texto completoLa instrumentación Point-of-care (POC) es compacta, móvil y permite una detección rápida, razón por la que se prevé que sean de gran ayuda en áreas como el diagnostico precoz, la monitorización de tratamientos y la medicina personalizada, revolucionando los modelos sanitarios, especialmente en las zonas de difícil acceso y con menos recursos. La necesidad de este tipo de dispositivos impulsa el desarrollo de novedosas tecnologías en el campo de los bio-sensores. Diseñar equipos para la detección de bio-moléculas que sean rápidos, pequeños y sencillos es un reto que requiere la integración de múltiples campos de la ciencia y la ingeniería. Los nano-resonadores ópticos muestran un gran potencial como bio-sensores sin necesidad de marcaje, gracias a su capacidad de acoplase directamente con la luz en modos menores que la longitud de onda. Los nano-resonadores metálicos basados en resonancias plasmónicas superficiales localizadas (LSPR) han sido estudiados y han demostrado ser una firme alternativa a los ya comerciales basados en resonancias plasmónicas superficiales (SPR). Los nano-resonadores dieléctricos han sido recientemente objeto de atención debido a sus bajas perdidas y la capacidad de manipular los componentes eléctricos y magnéticos de la luz. En esta tesis presentamos avances en el campo de la bio-detección y en el uso de los nano-resonadores ópticos como potenciales herramientas para la detección de enfermedades y monitorización de los tratamientos. Hemos desarrollado y evaluado distintas plataformas de detección combinando los nano-resonadores ópticos, tanto metálicos como dieléctricos, con las más avanzadas técnicas de microfluídica y química de superficies. En primer lugar, nos centramos en el desarrollo de un dispositivo microfluídico basado en sensores LSPR de oro que permite multiplexar 32 canales. Los 32 sensores se monitorizan en tiempo real para demostrar la cuantificación de 4 marcadores de cáncer de mama en suero sanguíneo humano. Demostramos que mediante la optimización de los ensayos se pueden alcanzar bajos límites de detección (LOD), lo que allana el camino hacia dispositivos POC de uso clínico. Por otro lado, hemos utilizado los nano-resonadores de silicio integrados con la microfluídica para también detectar marcadores de cáncer en suero. Estos sensores, cuyo principio de funcionamiento se basa en resonancias de MIE, han demostrado ser una alternativa razonable a los sensores de oro. Además, demostramos que un proceso de fabricación de nano-resonadores de silicio rápido, escalable y de bajo coste da lugar a límites de detección suficientes para la producción de futuras POC. También realizamos un minucioso estudio del rol de las resonancias eléctricas y magnéticas en dichos sensores y su relación con el desplazamiento y el cambio magnitud de la resonancia del sensor global. Nuestro trabajo es un avance en el desarrollo de futuros instrumentos POC rápidos y baratos en el ámbito de la salud a escala molecular.
Wang, Peng. "Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics". University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011.
Texto completoSanmartí, Espinal Marta. "Study of natural nanovesicles carrying olfactory receptors for the development of biosensing platforms". Doctoral thesis, Universitat de Barcelona, 2015. http://hdl.handle.net/10803/286002.
Texto completoVesícules naturals produïes a partir de cèl·lules modificades genèticament són prometedors components de sensat per utilitzar com a detectors en biodispositius. Això és particularment cert en el cas de receptors adjuntats a proteïna G (GPCRs) presents en molts processos cel·lulars, on la seva funcionalitat depèn estrictament del seu entorn lipídic. Els receptors de membrana estan involucrats en una gran varietat de vies bioquímiques i per tant són objectiu d’estudi per teràpia i desenvolupament de nous fàrmacs. Per tant, plataformes bioanalítiques i assajos d’unió receptor-lligand, utilitzant receptors transmembrana, requereixen la construcció de matrius de membranes lipídiques ben caracteritzades, actuant com a suport per evitar la desnaturalització de proteïnes durant el processament del bioxip. En aquesta tesi es presenta la producció i caracterització de nanovesícules de membrana (NV) provinents de cèl·lules de llevat Saccharomyces cerevisiae que contenen receptors olfactius (un membre de la família de GPCRs) heteròlogament expressats a la membrana. Hem demostrat que les fraccions de membrana, a partir de cèl·lules de llevat, en solució formen espontàniament nanovesícules esfèriques tancades. També s’ha demostrat, que després d’un procés de enginyeria genètica els receptors olfactius van ser expressats correctament a la membrana del llevat. També s’ha presentat un mètode simple per homogeneïtzar la mida de les nanovesícules. A més a més, es presenta per primer cop un nou mètode immunoquímic per la quantificació directa de les proteïnes transmembrana (GPCR) en el seu ambient lipídic natural. El mètode utilitza anticossos monoclonals en un assaig basat en ELISA amb alta detectabilitat. L’aplicació del mètode es demostra a través de la quantificació del receptors olfactius OR1740 i OR7D4 expressats en nanovesícules de membrana plasmàtica. També es presenta, mitjançant observació directa amb AFM, com les nanovesícules es depositen i s’aplanen sense trencar-se sobre substrats de vidre i or seguint la llei de difusió. Es demostra com en el cas del vidre els màxims recobriments superficials obtinguts són del 20-25% i en el cas del or funcionalitzat del 10-15%, controlant la concentració de nanovesícules, el temps de depòsit, la presència de residus procedents del procés de producció de les nanovesícules, la química de la superfície, la força iònica del medi, etc. Finalment, s’ha demostrat per SPR que els receptors expressats eren funcionals i que aquesta tècnica òptica permet la detecció de petites molècules, com són els odorants, a les concentracions en el rang micromolar. Els resultats presentats en aquesta tesis contribueixen donant un pas important a la realització de dispositius biosensors basats en nanovesícules naturals que integren receptors de membrana adjuntats a proteïna G.
Samiei, Ehsan. "Development of advanced operators for enhanced on-chip biosensing in digital microfluidic platforms". Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/60122.
Texto completoApplied Science, Faculty of
Engineering, School of (Okanagan)
Graduate
NOVARA, CHIARA. "Silver nanostructures on porous silicon for multiplexed Surface Enhanced Raman Scattering biosensing platforms". Doctoral thesis, Politecnico di Torino, 2016. http://hdl.handle.net/11583/2653123.
Texto completoNovikova, Irina V. "Paranemic and Receptor-Loop RNA Motifs: Versatile Interactions for Biosensing Platforms and Nanotechnology Scaffolds". Bowling Green State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1288300501.
Texto completoYang, Seung Ook. "Enzyme Encapsulation, Biosensing Endocrine Disrupting Chemicals, and Bio-therapeutic Expression Platforms Using Cell-Free Protein Synthesis". BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/6885.
Texto completoLin, Chih-Heng y 林志衡. "Silicon Nanowire Field-Effect Transistor Based Biosensing Platform". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/44441103558445344256.
Texto completo國立交通大學
生物科技學系
100
Real-time surveillance of the biomarker is critical for improvements in illness management and is especially important for early detection, rapid intervention, and a possible reduction of the disease occurrence. Enhanced surveillance requires rapid, robust, and inexpensive analytical techniques capable of providing a detailed analysis of biological molecules. A simple and low-cost method to fabricate poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) for bio-sensing application was demonstrated. The poly-silicon nanowire (poly-SiNW) channel was fabricated by employing the poly-silicon (poly-Si) sidewall spacer technique, which approach was comparable with current commercial semiconductor process and forsaken expensive electron beam (E-beam) lithography tools for large-scale production. The electronic properties of the poly-SiNW FET in aqueous solution were found to be similar to those of single-crystal SiNW FETs reported in the literature. Functionalized poly-SiNW FETs were used as the biosensors for specific and ultrasensitive detection of neurotransmitter dopamine and high pathogenic avian influenza virus DNA in this study. Specific electric changes were observed for dopamine and DNA sensing when nanowire surface of poly-SiNW FETs was modified with specific recognition capturers and those biological molecules at fM to pM range could be distinguished. We further demonstrated that specific detection, confirmation and recovery of DNA probe on the nanowire surface could be achieved with SiNW-FETs using hemagglutinin DNA as the diagnostic target. With its characteristics (ultrasensitive, label-free, and real-time detection) and advantages (potential for mass commercial production and integration with microfluidic system and circuit), poly-SiNW FET can be developed to become a portable biosensor for field use and point-of-care diagnoses.
Tu, Yi-Kai y 涂逸凱. "Integration of Photonic Crystal with Microfluidic Chip as Biosensing Platform". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/59690450204753572401.
Texto completo國立交通大學
機械工程系所
104
The goal of this project is to develop a novel Lab-on-a-chip (LOC) detection system. The proposed system consists of a microfluidic subsystem and a label-free (LF) detection subsystem. The system can facilitate sample preparation and provide immediate detections. The microfluidic subsystem consists of several components including two liquid inlet which can inject the samples and reagents, and filters for cell debris purification after lysis. All these components will be fabricated on a silicon chip simultaneously. Regarding the detection subsystem, we developed a sensitive photonic crystal (PC) biosensor for LF detection of proteins. The PC functions as an optical filter where only a particular combination of wavelength/illumination angle can excite the structure resonance, resulting in a strong reflection, while other combinations of wavelengths and illumination angles are transmitted through. Through monitoring the shift of the reflected wavelength, the concentration of the analyte can be determined. The designed LOC system was demonstrated to detect the Beta-actin proteins inside the cell and simultaneously to filter the cell debris. The result indicates the detection limit of 160 ng/ml can be achieved without any prefiltering procedure.
Huang, Yu-Chung y 黃昱中. "Development of Reflection-Based Tubular Waveguide Particle Plasmon Resonance Biosensing Platform". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/29jp5z.
Texto completo國立中正大學
化學暨生物化學研究所
102
The objective of this work is to develop a novel chemical and biochemical sensing platform, namely, a reflection-based tubular waveguide particle plasmon resonance (RTW-PPR) biosensing platform, which is based on the tubular waveguide particle plasmon resonance (TW-PPR) biosensor. The working principle of this invention is given by the following processes: 1. a light emitting diode (LED) emits light with an excitation wavelength corresponds to the particle plasmon resonance (PPR) of gold nanoparticle; 2. the light is transmitted by optical fibers and coupled into a glass tube; 3. the light traveled in the tube wall by multiple total internal reflections (TIRs); 4.The evanescent wave excites the PPR of gold nanoparticles on the surface of the tube wall. When the refractive index of the medium surrounding the nanoparticles changes (eg. adsorption of biomolecules on nanoparticle surface), the peak wavelength and extinction cross-section of the particle plasmon resonance (PPR) band changes. To construct the sensor tube, the bottom of the tube will be modified with a reflective layer. When the incident light reaches the reflective layer, the light will travel to opposite direction. The PPR effect increases through an increase of optical path length by reflection, when the sensor length is the same, thus effectively enhances the sensitivity of the sensing system. The light is finally detected by a photodiode (PD) through fiber optics. This system does not require labeling (eg. modified fluorescent molecular). Hence, the RTW-PPR biosensing platform can achieve label-free and real-time detection with high sensitivity. In this work, we optimized the optical and sensing elements of the system. System stability, reproducibility, sensor sensitivity and sensor resolution (SR) will be tested by a series of refractive index (RI) experiments and biochemical detection experiments. In the RI experiment, using different weight percents of sucrose in pure water, a refractive index resolution of 2.21x10-5 RIU and a sensor sensitivity of -6.17 RIU-1 have been achieved by the sensor. In the biochemical detection experiments, OVA were used to functionalize the gold nanoparticle in order to detect anti-OVA antibody. Results show that the calibration curve is linear (R2>0.99) and the limit of detection (LOD) is about 5.71x10-7 g/mL (3.81x10-9 M). In summary, we have developed a novel RTW-PPR biosensing platform successfully, and its feasibility in biosensing has been demonstrated.
Pagará, Beatriz Condeço Pinto. "Paper as a Colorimetric Biosensing Platform for Tetracyclines Detection in Milk". Master's thesis, 2018. http://hdl.handle.net/10362/61057.
Texto completoFan, Shu-Yu y 范書毓. "Fiber-optical biosensing platform for quantifying cell population or tissue level activities". Thesis, 2013. http://ndltd.ncl.edu.tw/handle/87907459896605344892.
Texto completo國立中興大學
生醫工程研究所
101
A fiber-optical biosensing platform in coordination with localized fluidic delivery to perform topical cell-scale assay inside living tissue or organs in vivo. The deviation from the cell density and spatial configuration in the vicinity of sensor probe can cause error in threshold value determination, which is difficult for the conventional extrinsic catheter fiber-optic designs to predict or calibrate for in vivo applications. The strategy for correcting / calibrating the difference from the spatial issue is to stain the cells with two fluorescent agents. The 1st fluorophore (indicator) will has a known effect on all cells which is not affected by the conditions (“normal / control” or “treated” with desired physiological changes) of the cells; the signal from the indicator will be considered as “baseline” reflecting each independent measurement with specific density and configuration of the cells in the vicinity of sensor tip. The 2nd fluorophore (reporter) will exhibit level of physiological change on “treated” cells. The significance of the physiological changes on individual cell will be evaluated by the ratio of the two fluorescent signals (reporter / indictor) to report the normalized deviation between “control” and “treated” cell population. In this research, we applied the fiber-optic sensor platform in monitoring chemotherapeutic, cyclophosphamide (CPA), induced 3D-distributed MCF-7 cell (human breast carcinoma cell line) apoptosis for verifying the feasibility and capability of the system in monitoring cell population or tissue level activities in vivo. In the 1st stage of the development, 25mM CPA was found to enhance apoptosis of cancer cells in cell viability (MTT) assay under 2D cell culture, increase the adsorption amount of fluorophore FM 1-43 on apoptotic cells, but had no effect on the adsorption amount of indocyanine derivatives on apoptotic cells. In the 2nd stage of study, the fiber-optic sensing platform monitored the kinetics of fluorescence changes around the micro-environment of sensor tip with a 200μm i.d. optical fiber, conveying the excitation and returning emission, and a 325μm i.d. microcapillary initially delivering fluorophore, 750nl 300 μm naphthalene asymmetric indocyanine derivative (Cpd.B), in indicating the spatial distribution (density) of cells in the tissue-mimic system, following by delivering fluorophore, 750nl 160μM FM1-43, in demonstrating the apoptotic activity induced by CPA after calibrated with the previous Cpd.B reported cell distribution indicating signal. When cell density exceeded 107cells/ml, the increase percentage of peak values in dynamic fluorescent change pattern of Cpd.B were in proportion to cell densities, but not correlated statistically with CPA-induced apoptotic activities. However, the increase percentages of peak values in dynamic fluorescent change pattern of FM 1-43 were in proportion to both cell densities and CPA-induced apoptotic activities. In the cell density between 107-108cells/ml, the increase percentage of peak values in dynamic fluorescent change pattern from FM 1-43 interacted with CPA-induced apoptotic cells were 2.1-3.3 folds of peak values from FM 1-43 interacted with control cell. When sequentially interacted with Cpd.B and FM 1-43, the ratios from increase percentage of peak values of FM 1-43 divided by increase percentage of peak values of Cpd.B were around 0.71-1.58 in the control cells with different densities, while the ratios from CPA-induced apoptotic cells were around 3.62-10.68. The value of ratio could be applied as indication of apoptotic activity without interference of spatial distribution of cells. The preliminary result verified the feasibility and capability of the system in monitoring cell population or tissue level activities in vivo.
Wu, Chia-Che y 吳嘉哲. "A Biosensing Platform Integrating ZnO Nanowires and Blood Plasma Separation Microfluidic Device". Thesis, 2013. http://ndltd.ncl.edu.tw/handle/93380828948571086184.
Texto completo國立交通大學
材料科學與工程學系奈米科技碩博士班
101
In this thesis, a biosensing platform integrating zinc oxide nanowires and blood plasma separation microfluidic device was demonstrated and investigated; Plasma microfluidic system utilizing gravity separation was prepared for effective filtering of the blood cells that usually caused signal interference in fluorescence intensity identification. The ZnO nanowire with biocompatibility nanopillar structure was optimized for the increase of the surface area in capture of target molecules. Prior to sensing, both Amino Propyl Triethoxy Silane (APTES) and Biotin were modified on the ZnO nanowire via self-assembly technique for Streptavidin detection. Whole blood samples spiked with various concentrations of Streptavidin were adopted to characterize the efficiency of the proposed system and the limit of detection (LOD). Fluoresence images confirmed the enhancement of the proposed sensing platform; with ZnO nanowire the LOD was enhanced from 42 pM to 4.2 pM. Finally, an automatic fluid flow mechanism was demonstrated to increase the feasibility of the proposed system. It is believed that by integrating these features the proposed platform can be beneficial to future point-of-care applications.
Chiu, Han-Sheng y 邱瀚生. "Fiber-optic Biosensing Platform for Real-time Evaluation of Chemotherapy Efficacy in vivo". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/30767871409190841473.
Texto completo國立中興大學
生醫工程研究所
102
A fiber-optical biosensing platform is developed to providing real-time evaluation of chemotherapy efficacy in vivo. It was verified by monitoring the apoptotic response of MDA-MB-231 xenografts in nu/nu mice induced by the maximal tolerance dosage of cyclophosphamide (CPA). Percutaneous 200μm i.d. optical fibers were applied to convey excitation and detect fluorescent emission from topically delivered FM1-43 (indicating apoptotic activities), and phospholipid modified marina blue (Mb, calibrating spatial distribution effect of cells).
Salgado, Shehan. "Graphene Encapsulation for Cells: A Bio-Sensing and Device Platform". Thesis, 2014. http://hdl.handle.net/10012/8391.
Texto completoLin, Chih-Hsiang y 林智翔. "C-reactive protein as the target model for development of a biorecognition and biosensing platform". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/j62re2.
Texto completo國立交通大學
應用化學系碩博士班
106
Single chain fragment variable (scFv), consists of heavy chain and light chain of fragment variable linked with flexible peptide, is the smallest unit of antibody with antigen-binding activities. Reduced size of protein facilitates the expression in E. coli. Phage display technology enables screening of scFv to against interested antigen. With the scFv expressed in E. coli, it has the potential to replace the use of antibodies produced by animal and consequently reduces the cost of diagnosis. C-reactive protein (CRP), the target antigens of this study, is recognized as a mediator of the acute-phase response, associated with various chronic inflammatory mechanism. CRP is a common biomarker in current assays. In this study, Maltose-binding protein fused CRP (MBP-CRP) expressed in E. coli was employed for scFv screening using a phage display library. Simultaneously, human cell produced CRP (CRPcell) served as the other target antigen for scFv screening. The scFv obtained from the screening, scFvCRP, was further inserted with his-tag at the N-terminus genetically to facilitate the process of protein purification. The affinity of scFvCRP was further characterized with indirect enzyme-linked immunosorbent assay (ELISA) and microscale thermophoresis (MST). Kd value was estimated to be 105 nM by indirect ELISA, whereas the Kd is 0.43 ± 0.06 nM obtained from MST analysis. The discrepancy between the two measurements becomes an interesting issue to be discussed. A detection platform was further established with the application of a NiO-coated chip on quartz crystal microbalance (QCM). Valid detection concentration was achieved below 100 nM (2.5 mg/L) of CRP.
Schmid, Matthew John. "Multi-analyte biosensing : the integration of sensing elements into a photolithographically constructed hydrogel based biosensor platform". Thesis, 2005. http://hdl.handle.net/2152/21921.
Texto completotext
Hsieh, Yi-Feng y 謝宜峯. "Modified Porous Carbon Spheres/Graphene Oxide Nanocomposites as a Sensitive Platform for Circulating Tumor Cells Biosensing". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/yhe4j6.
Texto completoWei, Lan-Yi y 魏蘭懿. "Development of protein tyrosine kinase activity biosensing platform and its application in anti-cancer drug test". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/x7u84u.
Texto completo國立交通大學
分子醫學與生物工程研究所
106
Protein tyrosine kinases are highly related to many human diseases, including neuron degenerative diseases, autoimmunity, diabetes, and cancers. Previously, a tyrosinase-based tyrosine kinase biosensor was developed and characterized by using Src as a model in our lab. In this study two other tyrosine kinases, Hck and Her2, were further explored and studied on a novel electrochemical detection setup with the previously developed tyrosine kinase sensing protocol. Hck protein kinase has been shown to be related to various immune disorders, such as chronic myelogenous leukemia, multiple myeloma and acute lymphatic leukemia. The dysfunction of Her2 protein kinase is strongly associated with the pathogenesis of various human cancers, including non-small cell lung cancer, ovarian cancer, pancreatic cancer, endometrial cancer and colon cancer. The platform allows reaction to be carried out in a small sample and with low noise. The results showed that the developed detection platform could be used for protein tyrosine kinases activity assay and for drug screening of these protein tyrosine kinases.
Wang, Chih-Ying y 王智瑩. "An Electrochemical Biosensing Platform Using Carbon Nanotubes Electrodes for Preclinical Evaluation of Drug Release Profile of Antibiotic Nanocapsules". Thesis, 2010. http://ndltd.ncl.edu.tw/handle/38940690324380035678.
Texto completo國立清華大學
動力機械工程學系
98
ii ABSTRACT In this thesis, an electrochemical biosensing platform using carbon nanotubes electrodes, which can be used to evaluate drug release profiles of antibiotic nanocapsules in real time and continuous mode, has been successfully designed, developed, and characterized. First, the electrochemical sensing electrode has been fabricated by MEMS technologies. Then, the biosensing platform is connected to the sensing circuit and LabVIEW program for signal acquire and processing. Finally, the developed carbon nanotubes electrode and electrochemical biosensing platform have been applied for pre-clinical evaluation of drug release profiles of antibiotic nanocapsules. The electrode is one of the key components for electrochemical sensing. The materials and the surface characteristics of electrodes play an important role in electrochemical sensing. Here, we successfully combined the carbon nanotubes electrodes with electrochemical biosensor, using electrophoresis deposition or drop-coating method to deposit carbon nanotubes on the surfaces of the gold electrodes. The measurement results show that the maximum affordable current has been improved 0.0208 mA to o.6680 mA. And, the sensing signals are amplified up to 13.75 times using carbon nanotube-modified electrodes. The linear range of the developed electrochemical biosensing platform using carbon nanotubes electrodes is from 1 g/ml to 10g/ml (R2=0.9837). The sensitivity of the developed system is 0.023 mA‧ml/g. The HPLC and other traditional instrument could not detection the drug release from nanocapsules in real time and continuous mode. According to the measurements using our developed electrochemical biosensing platform, it shows that antibiotic nanocapsules start to increase the drug release on the 4th day and the release rate is 0.0258 μg/ml.hr. The drug release of antibiotic nanocapsules reached 24.98 μg/ml on the 7th day. The antibiotic biosensor platform using carbon nanotube electrodes for preclinical evaluation of drug release profile of nanocapsules presented in this work showed good performance in sensing of antibiotic Teoplanin drug samples. The antibiotic biosensor platform could be further integrated with a micro fluidic platform for controlled synthesis of nanocapsules to feedback the drug release profile for optimization of the synthesis process. In addition, the developed biosensor can be integrated with wireless passive transmission module to be an implantable biomedical microsystem for health monitoring in future.
Wu, Jia-Xuan y 吳佳軒. "Phage Display Screening of Single-Chain Variable Fragments for proBrain Natriuretic Peptide Recognition and Development of Biosensing Platform". Thesis, 2019. http://ndltd.ncl.edu.tw/handle/9y6beq.
Texto completo國立交通大學
應用化學系分子科學碩博士班
108
The proBrain Natriuretic Peptide is a 108-amino acids prohormone secreted by cardiomyocytes in the heart ventricles in response to volume expansion and pressure overload. The proBrain Natriuretic Peptide can be cleaved by enzyme and produce N-terminal pro hormone BNP (76-amino acid) and BNP (32-amino acid). N-terminal prohormone BNP (NT-proBNP) is a non-active prohormone containing the first 76 amino acid residues of proBNP, Brain natriuretic peptide (BNP) is a active hormone including the remaining 32 amino acids. Both proBNP, BNP and NT-proBNP are produced resulting from the change of pressure inside the heart. Therefore, to measure the concentration of these proteins can be used for evaluation of heart failure. The proBNP was employed for phage display library screening. After several generations of the screening, two single-chain fragment variable (scFv) regions with high binding affinity against proBNP were obtained. The dissociation constant (Kd) of the two selected anti-proBNP scFvs was measured to be 3.4± 0.8 μM and 476 ± 38 nM via the application of microscale thermophoresis. The measurement of proBNP was further performed by electrochemical impedance spectroscopy. The measurement limit of proBNP using the anti-proBNP scFv1 is at μM level, which is not feasible for Kd value estumation, whereas the Kd of the anti-proBNP scFv2 platform towards proBNP is 74±14 nM. The linear range of proBNP was measured from 12.3 to 333 nM. Though the selected scFvs can recognize and specifically bind to proBNP, the binding affinity needs to be improved in order for establishing an effective detection of proBNP.
Holmes, Richard. "Towards a Novel Electrochemical Sensing Platform for Diagnosing Urinary Tract Infections". Thesis, 2012. http://hdl.handle.net/1807/33242.
Texto completoYang, Wan-Ling y 楊婉鈴. "Applications of silicon nanowire field-effect transistors on biochemistry study: 1. Minimizing sample volume and detection time via selective surface modification 2. Coupling supported lipid bilayer to a silicon nanowire transistor as a biosensing platform". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/32544513155555116051.
Texto completo國立臺灣大學
化學研究所
100
Silicon nanowire field-effect transistors (SiNW-FETs) have drawn great attention because of their potential as a label-free, real-time, and ultra-sensitive sensor for biomolecular detections. As a biological sensor, the surface of a SiNW-FET device was conventionally all area modified (AAM) with receptors, covering not only the minute SiNW surface area but also the relatively massive surrounding substrate area. However, target molecules could be captured on the upstream substrate area before reaching the SiNW surface in sensing measurements, thus jeopardizing the detection sensitivity. In this study, we have successfully fabricated SiNW-FETs with the selective surface modification (SSM) of receptors only on the SiNW sensing surface via gas-phase premodification and a bottom-up fabrication technique. Our results show that a SSM SiNW-FET, exhibiting desirable electrical characteristics with regard to ohmic contact and high transconductance, has the merits of faster response time, less sample requirements, and much improved detection sensitivity. Besides, we integrated SiNW-FET with a lipid bilayer to mimic the cell membrane for biological research, especially for the membrane protein studies. Our results show that a 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid bilayer membrane with single or double lipid bilayers could be homogeneously formed on the SiNW-FET surface via a vesicle fusion method. However, because the shielding of the lipid bilayers on the underlying SiNW, signals were reduced in electrical measurement. To improve the signal acquisition from a lipid bilayer membrane covered SiNW-FET, we demonstrated that the electrical signals and the detection limit can be enhanced by utilizing a multiple-parallel-connection (MPC) SiNW-FET system.
LIN, YUCHENG y 林祐正. "Development of Reflective Guided-Mode-Resonance Biosensing Platforms". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/55933647000058942336.
Texto completo國立中正大學
機械工程系研究所
104
The present study develops a low-cost biochip system employing guided-mode resonance biochip. The advantage of the guided-mode resonance biochip lies in its simple structure and the fact that it is a label-free biochip. By altering the geometric structure of the guided-mode resonance biochip, the sensitivity may be increased over 200 nm/RIU, and thus applicable for high-sensitivity bio-chemical detection. The present study first discussed the impact the geometrical effect has on sensitivity of biochips, and whether it could increase the sensitivity of guided-mode resonance biochip. We perform finite element simulation and transmission experiment to study the impact that depth and thickness of gratings have on sensitivity. The result of finite element analysis shows that, for both thickness and depth, sensitivity peaks at a certain value; increase or decrease from that particular value would cause the sensitivity to drop. Further, it is noticeable that when the geometric size of grating was optimized, the chip’s sensitivity is five times higher than those were not. In other words, optimizing the geometric size of the chip may increase its sensitivity by fivefold. Combining this result to other effects, the performance of guided-mode resonance biochip could be significantly improved. The present study then turned to developing a low-cost biochip detection system. We employed LED as light source, and used the variation in light intensity as detection mechanism to compare the performances of transmissive and reflective structure. The present study found that the reflective light intensity measurement system shows better performance than the transmissive light intensity measurement system, where the resolution of the reflective system, comparing to that of the transmissive system, is increased by approximately 427%. In order to proof the reflective system’s capability in bio-chemical detection, the present study also conducted a DNP/anti-DNP bio-chemical detection experiment. And the result of the calculations shows a detection limit of 5.38×10^(-8) g⁄ml for the reflective guided-mode resonance biochip that we developed, which proves it to possess outstanding bio-signal detection capability.
Ahl, Stefanie Elisabeth [Verfasser]. "New platforms for optical biosensing / Stefanie Elisabeth Ahl". 2007. http://d-nb.info/985594470/34.
Texto completoSANG, JIA-YU y 宋家裕. "Development of Reflection-Based Optical Waveguide Particle Plasmon Resonance Biosensing Platforms". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/68801620211485505316.
Texto completo國立中正大學
化學暨生物化學研究所
104
The objectives of this work are to develop two novel multiplex chemical and biochemical sensing platforms, namly a reflection-based tubular waveguide particle plasmon resonance (RTW-PPR) biosensing platform, and a reflection-based fiber optic particle plasmon resonance (RFO-PPR) biosensing platform. The principle of inventions are based on measuring the light intensity after consecutive total internal reflections (TIRs) along a noble metal nanoparticles-modified waveguide (tube or optical fiber), wherein the evanescent wave excites the particle plasmon resonance of the nanoparticles at the reflection interface. When a noble metal nanoparticle is influenced by the change of the refractive index on its surrounding environment, its particle plasmon resonance condition will change. This phenomenon can be used as the basis of chemical and biological sensing. In the first part :we used Poly(methyl methacrylate) PMMA as waveguide material to form a tubular waveguide and utilized 3-mercaptopropylsilatrane (MPS) to reduce the modification time. A variety of experiments were carried out to validate the sensitivity and refractive index resolution of the sensing platform. Using different weight percent of sucrose in pure water as samples, a refractive index resolution of 4.34×10-5 RIU and a sensor sensitivity of 5.39 RIU-1 have been achieved by the platform. In the biochemical detection experiments, OVA was used to functionalize the gold nanoparticle in order to detect anti-OVA. Results show that the calibration curve is linear (R2>0.99) and the limit of detection (LOD) is about 4.64×10-6 g/mL (3.09×10-8 M). In the second part:the RFO-PPR platform has achieved the absorbance sensitivity of 4.83 AU/RIU-1 and the sensor resolution of 4.6×10-5 RIU by using gold nanospheres as the sensing element. By the similar configuration, but using gold nanorods as the sensing element, the absorbance sensitivity of 3.81 AU/RIU-1 and the sensor resolution of 3.7×10-5 RIU have been achieved. In the biochemical detection experiments, DNP was used to functionalize the gold nanorods in order to detect anti-DNP antibody. Results show that the calibration curve is linear (correlation coefficient >0.99) and the detection limit is less than 3.88×10-10 M.
Ong, Peijie. "Optimization and characterization of noise for ion channel and carbon nanotube biosensing platforms". Thesis, 2020. https://doi.org/10.7916/d8-h8mz-zd83.
Texto completoChang, Jhih-Wei y 張志瑋. "Applications of Label-Free Biosensing and Development of Low-Cost Plasmonic Sensing Platforms". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/03793057109094689392.
Texto completo國立陽明大學
生醫光電研究所
102
Nanostructure-based surface plasmon resonance sensors are capable of sensitive and label-free detection for chemical and biological sensing applications. However, low-cost mass-production techniques and development of portable low-cost sensing platforms are the main issues which should be addressed. In this study, double-layer gold nanoslit arrays were fabricated on a cyclic olefin polymer (COP) film using hot embossing nanoimprinting lithography and metal sputtering techniques and then utilized to detect methicillin-resistant staphylococcus aureus (MRSA). In the experiment, penicillin-Binding Protein 2α present in MRSA was detected using the plasmonic biochips and the minimum detectable concentration of penicillin-Binding Protein 2α was 100 ng/mL. In order to improve the sensitivity of the biochips, double-layer gold nanoslit array with a period of 1000 nm was fabricated and tested. The result shows that the wavelength sensitivity of the chip was 926 nm/RIU and the figure of merit value was up to 272. In addition, we combined an inexpensive transmission-type scanner, a 632.8 nm laser line filter and plasmonic biochips to establish a portable low-cost sensing platform capable of high-throughput detection. An antigen-antibody interaction experiment in aqueous environment was conducted using the platform to verify the detection sensitivity in surface binding event. The proposed sensing platform has the advantages of label-free high-throughput detection, simple operation method, quick detection, low price and portable. It can benefit various sensing applications and is suitable for point-of-care detection.
(5930402), Justin C. Wirth. "Engineering Sensitivity: An Optical Optimization of Ring Resonator Arrays for Label-Free Whole Bacterial Sensing". Thesis, 2019.
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