Journal articles: 'Zebrafish model system'

1

Belyaeva, N. F., V. N. Kashirtseva, N. V. Medvedeva, Yu Yu Khudoklinova, O. M. Ipatova, and A. I. Archakov. "Zebrafish as a model system for biomedical studies." Biomeditsinskaya Khimiya 56, no. 1 (January 2010): 120–31. http://dx.doi.org/10.18097/pbmc20105601120.

Full text

Abstract:

Zebrafish (Danio rerio) are now firmly established as a powerful research model for many areas of biology and medicine. Here, we review some achievements of zebrafish - based assays for modeling human diseases and for drug discovery and development. For drug discovery, zebrafish are especially valuable in the earlier stages of research as they provide a model organism to demonstrate a new treatment's efficacy and toxicity before more costly mammalian models are used. This review provides examples of compounds known to be toxic to humans that have been demonstrated to functional similarly in zebrafish. Major advantages of zebrafish embryons are that they are readily permeable to small molecules added to their incubation medium and the transparent chorion enables the easy observation of development. Assay of acute toxicity (LC50 estimation) in embryos can also include the screening for developmental disorders as an indicator of teratogenic effects. We used zebrafish for toxicity testing of new drugs on the base of phospholipid nanoparticles. The organization of the genome and the pathways controlling signal transduction appear to be highly conserved between zebrafish and humans that allow using zebrafish for modeling of human diseases some examples of which are illustrated in this paper.

APA, Harvard, Vancouver, ISO, and other styles

2

Wasel, Ola, and Jennifer L. Freeman. "Chemical and Genetic Zebrafish Models to Define Mechanisms of and Treatments for Dopaminergic Neurodegeneration." International Journal of Molecular Sciences 21, no. 17 (August 20, 2020): 5981. http://dx.doi.org/10.3390/ijms21175981.

Full text

Abstract:

The zebrafish (Danio rerio) is routinely used in biological studies as a vertebrate model system that provides unique strengths allowing applications in studies of neurodevelopmental and neurodegenerative diseases. One specific advantage is that the neurotransmitter systems are highly conserved throughout vertebrate evolution, including between zebrafish and humans. Disruption of the dopaminergic signaling pathway is linked to multiple neurological disorders. One of the most common is Parkinson’s disease, a neurodegenerative disease associated with the loss of dopaminergic neurons, among other neuropathological characteristics. In this review, the development of the zebrafish’s dopaminergic system, focusing on genetic control of the dopaminergic system, is detailed. Second, neurotoxicant models used to study dopaminergic neuronal loss, including 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), the pesticides paraquat and rotenone, and 6-hydroxydopamine (6-OHDA), are described. Next, zebrafish genetic knockdown models of dj1, pink1, and prkn established for investigating mechanisms of Parkinson’s disease are discussed. Chemical modulators of the dopaminergic system are also highlighted to showcase the applicability of the zebrafish to identify mechanisms and treatments for neurodegenerative diseases such as Parkinson’s disease associated with the dopaminergic system.

APA, Harvard, Vancouver, ISO, and other styles

3

Yoder, Jeffrey A., Michael E. Nielsen, Chris T. Amemiya, and Gary W. Litman. "Zebrafish as an immunological model system." Microbes and Infection 4, no. 14 (November 2002): 1469–78. http://dx.doi.org/10.1016/s1286-4579(02)00029-1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Amatruda, James F., Jennifer L. Shepard, Howard M. Stern, and Leonard I. Zon. "Zebrafish as a cancer model system." Cancer Cell 1, no. 3 (April 2002): 229–31. http://dx.doi.org/10.1016/s1535-6108(02)00052-1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Goldsmith, J. R., and Christian Jobin. "Think Small: Zebrafish as a Model System of Human Pathology." Journal of Biomedicine and Biotechnology 2012 (2012): 1–12. http://dx.doi.org/10.1155/2012/817341.

Full text

Abstract:

Although human pathologies have mostly been modeled using higher mammal systems such as mice, the lower vertebrate zebrafish has gained tremendous attention as a model system. The advantages of zebrafish over classical vertebrate models are multifactorial and include high genetic and organ system homology to humans, high fecundity, external fertilization, ease of genetic manipulation, and transparency through early adulthood that enables powerful imaging modalities. This paper focuses on four areas of human pathology that were developed and/or advanced significantly in zebrafish in the last decade. These areas are (1) wound healing/restitution, (2) gastrointestinal diseases, (3) microbe-host interactions, and (4) genetic diseases and drug screens. Important biological processes and pathologies explored include wound-healing responses, pancreatic cancer, inflammatory bowel diseases, nonalcoholic fatty liver disease, and mycobacterium infection. The utility of zebrafish in screening for novel genes important in various pathologies such as polycystic kidney disease is also discussed.

APA, Harvard, Vancouver, ISO, and other styles

6

Bilotta, Joseph, and Shannon Saszik. "The zebrafish as a model visual system." International Journal of Developmental Neuroscience 19, no. 7 (November 2001): 621–29. http://dx.doi.org/10.1016/s0736-5748(01)00050-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Pitchai, Arjun, Rajesh Kannan Rajaretinam, and Jennifer L. Freeman. "Zebrafish as an Emerging Model for Bioassay-Guided Natural Product Drug Discovery for Neurological Disorders." Medicines 6, no. 2 (May 30, 2019): 61. http://dx.doi.org/10.3390/medicines6020061.

Full text

Abstract:

Most neurodegenerative diseases are currently incurable, with large social and economic impacts. Recently, there has been renewed interest in investigating natural products in the modern drug discovery paradigm as novel, bioactive small molecules. Moreover, the discovery of potential therapies for neurological disorders is challenging and involves developing optimized animal models for drug screening. In contemporary biomedicine, the growing need to develop experimental models to obtain a detailed understanding of malady conditions and to portray pioneering treatments has resulted in the application of zebrafish to close the gap between in vitro and in vivo assays. Zebrafish in pharmacogenetics and neuropharmacology are rapidly becoming a widely used organism. Brain function, dysfunction, genetic, and pharmacological modulation considerations are enhanced by both larval and adult zebrafish. Bioassay-guided identification of natural products using zebrafish presents as an attractive strategy for generating new lead compounds. Here, we see evidence that the zebrafish’s central nervous system is suitable for modeling human neurological disease and we review and evaluate natural product research using zebrafish as a vertebrate model platform to systematically identify bioactive natural products. Finally, we review recently developed zebrafish models of neurological disorders that have the potential to be applied in this field of research.

APA, Harvard, Vancouver, ISO, and other styles

8

Maves, Lisa. "Special Issue “Zebrafish-A Model System for Developmental Biology Study”." Journal of Developmental Biology 8, no. 3 (August 4, 2020): 15. http://dx.doi.org/10.3390/jdb8030015.

Full text

Abstract:

For this Special Issue “Zebrafish-A Model System for Developmental Biology Study,” we present a collection of studies, including original research papers and review articles, that focus on advances in developmental biology research and that take advantage of the zebrafish model organism [...]

APA, Harvard, Vancouver, ISO, and other styles

9

Nam, Youn Hee, Isabel Rodriguez, Sung Woo Shin, Ji Heon Shim, Na Woo Kim, Min Cheol Kim, Seo Yule Jeong, et al. "Characteristics of the New Insulin-Resistant Zebrafish Model." Pharmaceuticals 14, no. 7 (July 4, 2021): 642. http://dx.doi.org/10.3390/ph14070642.

Full text

Abstract:

Insulin resistance, which occurs when insulin levels are sufficiently high over a prolonged period, causing the cells to fail to respond normally to the hormone. As a system for insulin resistance and diabetes drug development, insulin-resistant rodent models have been clearly established, but there is a limitation to high-throughput drug screening. Recently, zebrafish have been identified as an excellent system for drug discovery and identification of therapeutic targets, but studies on insulin resistance models have not been extensively performed. Therefore, we aimed to make a rapid insulin-resistant zebrafish model that complements the existing rodent models. To establish this model, zebrafish were treated with 10 μM insulin for 48 h. This model showed characteristics of insulin-resistant disease such as damaged pancreatic islets. Then we confirmed the recovery of the pancreatic islets after pioglitazone treatment. In addition, it was found that insulin-resistant drugs have as significant an effect in zebrafish as in humans, and these results proved the value of the zebrafish insulin resistance model for drug selection. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that insulin resistance altered gene expression due to the MAPK signaling and calcium signaling pathways. This model demonstrates the utility of the zebrafish model for drug testing and drug discovery in insulin resistance and diabetes.

APA, Harvard, Vancouver, ISO, and other styles

10

Elo, B., C. M. Villano, D. Govorko, and L. A. White. "Larval zebrafish as a model for glucose metabolism: expression of phosphoenolpyruvate carboxykinase as a marker for exposure to anti-diabetic compounds." Journal of Molecular Endocrinology 38, no. 4 (April 2007): 433–40. http://dx.doi.org/10.1677/jme-06-0037.

Full text

Abstract:

The zebrafish model system is one of the most widely used animal models for developmental research and it is now becoming an attractive model for drug discovery and toxicological screening. The completion of sequencing the zebrafish genome and the availability of full-length cDNAs and DNA microarrays for expression analysis, in addition to techniques for generating transgenic lines and targeted mutations, have made the zebrafish model even more attractive to researchers. Recent data indicate that the regulation of glucose metabolism in zebrafish, through the production of insulin, is similar to mammalian models, and many of the genes involved in regulating blood glucose levels have been identified in zebrafish. The data presented here show that adult zebrafish respond to anti-diabetic drugs similarly to mammalian models, by reducing blood glucose levels. Furthermore, we show that the expression of phosphoenolpyruvate carboxykinase (PEPCK), which catalyzes a rate-limiting step in gluconeogenesis and is transcriptionally regulated by glucagon and insulin, is regulated in larval zebrafish similarly to that seen in mammalian systems, and changes in PEPCK expression can be obtained through real-time PCR analysis of whole larval RNA. Taken together, these data suggest that larval zebrafish may be an appropriate model for the examination of glucose metabolism, using PEPCK as an indicator of blood glucose levels.

APA, Harvard, Vancouver, ISO, and other styles

11

Posner, Mason, Kelly L. Murray, Matthew S. McDonald, Hayden Eighinger, Brandon Andrew, Amy Drossman, Zachary Haley, Justin Nussbaum, Larry L. David, and Kirsten J. Lampi. "The zebrafish as a model system for analyzing mammalian and native α-crystallin promoter function." PeerJ 5 (November 27, 2017): e4093. http://dx.doi.org/10.7717/peerj.4093.

Full text

Abstract:

Previous studies have used the zebrafish to investigate the biology of lens crystallin proteins and their roles in development and disease. However, little is known about zebrafish α-crystallin promoter function, how it compares to that of mammals, or whether mammalian α-crystallin promoter activity can be assessed using zebrafish embryos. We injected a variety of α-crystallin promoter fragments from each species combined with the coding sequence for green fluorescent protein (GFP) into zebrafish zygotes to determine the resulting spatiotemporal expression patterns in the developing embryo. We also measured mRNA levels and protein abundance for all three zebrafish α-crystallins. Our data showed that mouse and zebrafish αA-crystallin promoters generated similar GFP expression in the lens, but with earlier onset when using mouse promoters. Expression was also found in notochord and skeletal muscle in a smaller percentage of embryos. Mouse αB-crystallin promoter fragments drove GFP expression primarily in zebrafish skeletal muscle, with less common expression in notochord, lens, heart and in extraocular regions of the eye. A short fragment containing only a lens-specific enhancer region increased lens and notochord GFP expression while decreasing muscle expression, suggesting that the influence of mouse promoter control regions carries over into zebrafish embryos. The two paralogous zebrafish αB-crystallin promoters produced subtly different expression profiles, with the aBa promoter driving expression equally in notochord and skeletal muscle while the αBb promoter resulted primarily in skeletal muscle expression. Messenger RNA for zebrafish αA increased between 1 and 2 days post fertilization (dpf), αBa increased between 4 and 5 dpf, but αBb remained at baseline levels through 5 dpf. Parallel reaction monitoring (PRM) mass spectrometry was used to detect αA, aBa, and αBb peptides in digests of zebrafish embryos. In whole embryos, αA-crystallin was first detected by 2 dpf, peaked in abundance by 4–5 dpf, and was localized to the eye. αBa was detected in whole embryo at nearly constant levels from 1–6 dpf, was also localized primarily to the eye, and its abundance in extraocular tissues decreased from 4–7 dpf. In contrast, due to its low abundance, no αBb protein could be detected in whole embryo, or dissected eye and extraocular tissues. Our results show that mammalian α-crystallin promoters can be efficiently screened in zebrafish embryos and that their controlling regions are well conserved. An ontogenetic shift in zebrafish aBa-crystallin promoter activity provides an interesting system for examining the evolution and control of tissue specificity. Future studies that combine these promoter based approaches with the expanding ability to engineer the zebrafish genome via techniques such as CRISPR/Cas9 will allow the manipulation of protein expression to test hypotheses about lens crystallin function and its relation to lens biology and disease.

APA, Harvard, Vancouver, ISO, and other styles

12

K. Shrman, Kamal Kishor, R. P. Singh N. S. Meena, and Neelam Dinodia Rituraj Kewat. "Zebrafish: An Emerging Model System in Toxicological Studies." International Journal of Current Microbiology and Applied Sciences 10, no. 2 (February 10, 2021): 826–35. http://dx.doi.org/10.20546/ijcmas.2021.1002.098.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Wen, Xiao-Yan. "Zebrafish: A Renewed Model System For Functional Genomics." Science Foundation in China 16, no. 2 (January 1, 2008): 36–46. http://dx.doi.org/10.1088/1005-0841/16/2/004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Belyaeva, N. F., V. N. Kashirtseva, N. V. Medvedeva, Yu Yu Khudoklinova, O. M. Ipatova, and A. I. Archakov. "Zebrafish as a model system for biomedical studies." Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry 3, no. 4 (November 17, 2009): 343–50. http://dx.doi.org/10.1134/s1990750809040039.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Dai, Yu-Jie, Yong-Fang Jia, Na Chen, Wan-Ping Bian, Qin-Kai Li, Yan-Bo Ma, Yan-Ling Chen, and De-Sheng Pei. "Zebrafish as a model system to study toxicology." Environmental Toxicology and Chemistry 33, no. 1 (December 4, 2013): 11–17. http://dx.doi.org/10.1002/etc.2406.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Teoh, Seong Lin, Shaiful Ridzwan Bin Sapri, Mohd Rafizul Bin Mohd Yusof, Mohamad Fairuz Yahaya, and Srijit Das. "Construction of an Affordable Open-Design Recirculating Zebrafish Housing System." Journal of the American Association for Laboratory Animal Science 59, no. 5 (September 1, 2020): 512–18. http://dx.doi.org/10.30802/aalas-jaalas-19-000167.

Full text

Abstract:

Recently, the zebrafish has gained in popularity as a vertebrate animal model for biomedical research. Commercial zebrafish housing systems are available and are designed to maximize stocking density of fish for a given space, but these systems are expensive and purchasing them may not be feasible for emerging laboratories with limited funding. In this article, we describe the construction of a simple and affordable recirculating zebrafish housing system. This system can be constructed in 3 working days, with materials readily available in hardware stores. The cost for construction of the system was only 3,000 MYR (750 USD). The system consists of a water reservoir, a supply line that delivers water to the shelves holding the zebrafish tanks, and a drainage line that receives water from both the supply line and the shelves containing the fish tanks and returns this water to the reservoir. This system also has a 3-stage filtration process to ensure that clean water is delivered to the zebrafish tank. The system can house up to 360 zebrafish. This low-cost housing system may make research using zebrafish feasible some laboratories.

APA, Harvard, Vancouver, ISO, and other styles

17

Montes, Guilherme Carneiro. "The use of zebrafish to evaluate neuropharmacology of the gold nanoparticles." European Journal of Chemistry 12, no. 4 (December 31, 2021): 488–92. http://dx.doi.org/10.5155/eurjchem.12.4.488-492.2152.

Full text

Abstract:

Zebrafish (Danio rerio) is a vertebrate animal used in animal model research with complex brains and behaviors similar to humans and associate with low coast become a model attractive for the academic community to seek zebrafish for scientific research. Studies on diseases of the central nervous system (CNS) have advanced and news therapeutic agents were developed for treatment these disorders. Reports suggest that the zebrafish model supports the neurodegenerative studies due functional conservation between human genes implicated in neurodegenerative disorders. The discovery of therapeutic compounds for CNS using the zebrafish model allows to show a neuroprotective action or neurotoxicity that might alter the behavioral changes. Neurotoxicity tests might perform in zebrafish’s embryos into 96 multi-well plates, which reduces the amount of substances used and cost. The bioactive compounds able to penetrate the blood-brain barrier (BBB) have important role physicochemical properties that might be desirable pharmacological effects and zebrafish trials allow if the substances might penetrate BBB and to exert central activity. The assays zebrafish are used to analyze nanoparticles that are small molecules used to explore variety applications in human health. Gold nanoparticles (AuNPs) has important properties which are extremely interest for pharmaceutical area such as drug delivery, cellular imaging, diagnostics, and therapeutic agents. Gold nanoparticles enhances Parkinson symptoms and improved neuroinflammation. Some studies show zebrafish might use to evaluate gold nanoparticles for human health hazard and toxicity studies. There is enormous potential for zebrafish in preclinical assays due to predict pharmacological and toxicity effects. Specific guidelines focused on methodologies in the zebrafish are needed to ensure adequate reproducible trials.

APA, Harvard, Vancouver, ISO, and other styles

18

Francoeur, Nicholas, and Rwik Sen. "Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research." Journal of Developmental Biology 9, no. 4 (September 25, 2021): 40. http://dx.doi.org/10.3390/jdb9040040.

Full text

Abstract:

Heart disease is the leading cause of death in the United States and worldwide. Understanding the molecular mechanisms of cardiac development and regeneration will improve diagnostic and therapeutic interventions against heart disease. In this direction, zebrafish is an excellent model because several processes of zebrafish heart development are largely conserved in humans, and zebrafish has several advantages as a model organism. Zebrafish transcriptomic profiles undergo alterations during different stages of cardiac development and regeneration which are revealed by RNA-sequencing. ChIP-sequencing has detected genome-wide occupancy of histone post-translational modifications that epigenetically regulate gene expression and identified a locus with enhancer-like characteristics. ATAC-sequencing has identified active enhancers in cardiac progenitor cells during early developmental stages which overlap with occupancy of histone modifications of active transcription as determined by ChIP-sequencing. CRISPR-mediated editing of the zebrafish genome shows how chromatin modifiers and DNA-binding proteins regulate heart development, in association with crucial signaling pathways. Hence, more studies in this direction are essential to improve human health because they answer fundamental questions on cardiac development and regeneration, their differences, and why zebrafish hearts regenerate upon injury, unlike humans. This review focuses on some of the latest studies using state-of-the-art technology enabled by the elegant yet simple zebrafish.

APA, Harvard, Vancouver, ISO, and other styles

19

Derzhavina, K. A., N. P. Ilyin, M. V. Seredinskaya, M. O. Nerush, K. V. Zakharchenko, D. V. Sorokin, K. A. Demin, and A. V. Kalueff. "Zebrafish as a model organism for rare diseases of nervous system." Russian Journal for Personalized Medicine 2, no. 2 (June 1, 2022): 17–32. http://dx.doi.org/10.18705/2782-3806-2022-2-2-17-32.

Full text

Abstract:

Rare (orphan) diseases are an urgent unmet biomedical problem. Tremendous efforts and resources are expended for the search for cures for these diseases. Zebrafish can provide a powerful screening tool for novel orphan drugs, and may also deepen our mechanistic understanding of such rare pathological conditions. Here, we discuss various models of orphan diseases of the nervous system on zebrafish, and outline associated problems, limitations and prospects in the context of the development of personalized medicine.

APA, Harvard, Vancouver, ISO, and other styles

20

Pudelko, Linda, Steven Edwards, Mirela Balan, Daniel Nyqvist, Jonathan Al-Saadi, Johannes Dittmer, Ingrid Almlöf, Thomas Helleday, and Lars Bräutigam. "An orthotopic glioblastoma animal model suitable for high-throughput screenings." Neuro-Oncology 20, no. 11 (May 10, 2018): 1475–84. http://dx.doi.org/10.1093/neuonc/noy071.

Full text

Abstract:

Abstract Background Glioblastoma (GBM) is an aggressive form of brain cancer with poor prognosis. Although murine animal models have given valuable insights into the GBM disease biology, they cannot be used in high-throughput screens to identify and profile novel therapies. The only vertebrate model suitable for large-scale screens, the zebrafish, has proven to faithfully recapitulate biology and pathology of human malignancies, and clinically relevant orthotopic zebrafish models have been developed. However, currently available GBM orthotopic zebrafish models do not support high-throughput drug discovery screens. Methods We transplanted both GBM cell lines as well as patient-derived material into zebrafish blastulas. We followed the behavior of the transplants with time-lapse microscopy and real-time in vivo light-sheet microscopy. Results We found that GBM material transplanted into zebrafish blastomeres robustly migrated into the developing nervous system, establishing an orthotopic intracranial tumor already 24 hours after transplantation. Detailed analysis revealed that our model faithfully recapitulates the human disease. Conclusion We have developed a robust, fast, and automatable transplantation assay to establish orthotopic GBM tumors in zebrafish. In contrast to currently available orthotopic zebrafish models, our approach does not require technically challenging intracranial transplantation of single embryos. Our improved zebrafish model enables transplantation of thousands of embryos per hour, thus providing an orthotopic vertebrate GBM model for direct application in drug discovery screens.

APA, Harvard, Vancouver, ISO, and other styles

21

Chao, Chun-Cheih, Po-Chen Hsu, Chung-Feng Jen, I.-Hui Chen, Chieh-Huei Wang, Hau-Chien Chan, Pei-Wen Tsai, et al. "Zebrafish as a Model Host for Candida albicans Infection." Infection and Immunity 78, no. 6 (March 22, 2010): 2512–21. http://dx.doi.org/10.1128/iai.01293-09.

Full text

Abstract:

ABSTRACTIn this work, the zebrafish model organism was developed to obtain a minivertebrate host system for aCandida albicansinfection study. We demonstrated thatC. albicanscan colonize and invade zebrafish at multiple anatomical sites and kill the fish in a dose-dependent manner. Inside zebrafish, we monitored the progression of theC. albicansyeast-to-hypha transition by tracking morphogenesis, and we monitored the corresponding gene expression of the pathogen and the early host immune response. We performed a zebrafish survival assay with differentC. albicansstrains (SC5314, ATCC 10231, anhgc1mutant, and acph1/efg1double mutant) to determine each strain's virulence, and the results were similar to findings reported in previous mouse model studies. Finally, using zebrafish embryos, we monitoredC. albicansinfection and visualized the interaction between pathogen and host myelomonocytic cellsin vivo. Taken together, the results of this work demonstrate that zebrafish can be a useful host model to studyC. albicanspathogenesis, and they highlight the advantages of using the zebrafish model in future invasive fungal research.

APA, Harvard, Vancouver, ISO, and other styles

22

Najib, Nor H. M., Yong H. Nies, Syarifah A. S. Abd Halim, Mohamad F. Yahaya, Srijit Das, Wei L. Lim, and Seong L. Teoh. "Modeling Parkinson’s Disease in Zebrafish." CNS & Neurological Disorders - Drug Targets 19, no. 5 (November 13, 2020): 386–99. http://dx.doi.org/10.2174/1871527319666200708124117.

Full text

Abstract:

Parkinson’s Disease (PD) is one of the most common neurodegenerative disorders that affects the motor system, and includes cardinal motor symptoms such as resting tremor, cogwheel rigidity, bradykinesia and postural instability. Its prevalence is increasing worldwide due to the increase in life span. Although, two centuries since the first description of the disease, no proper cure with regard to treatment strategies and control of symptoms could be reached. One of the major challenges faced by the researchers is to have a suitable research model. Rodents are the most common PD models used, but no single model can replicate the true nature of PD. In this review, we aim to discuss another animal model, the zebrafish (Danio rerio), which is gaining popularity. Zebrafish brain has all the major structures found in the mammalian brain, with neurotransmitter systems, and it also possesses a functional blood-brain barrier similar to humans. From the perspective of PD research, the zebrafish possesses the ventral diencephalon, which is thought to be homologous to the mammalian substantia nigra. We summarize the various zebrafish models available to study PD, namely chemical-induced and genetic models. The zebrafish can complement the use of other animal models for the mechanistic study of PD and help in the screening of new potential therapeutic compounds.

APA, Harvard, Vancouver, ISO, and other styles

23

Hentschel, Dirk M., Kwon Moo Park, Lucia Cilenti, Antonis S. Zervos, Iain Drummond, and Joseph V. Bonventre. "Acute renal failure in zebrafish: a novel system to study a complex disease." American Journal of Physiology-Renal Physiology 288, no. 5 (May 2005): F923—F929. http://dx.doi.org/10.1152/ajprenal.00386.2004.

Full text

Abstract:

Acute renal failure (ARF) is characterized by a very high mortality essentially unchanged over the past 40 years. Simple vertebrate models are needed to improve our understanding of ARF and facilitate the development of novel therapies for this clinical syndrome. Here, we demonstrate that gentamicin, a commonly used nephrotoxic antibiotic, causes larval zebrafish to develop ARF characterized by histological and functional changes that mirror aminoglycoside toxicity in higher vertebrates and inability of zebrafish to maintain fluid homeostasis. We developed a novel method to quantitate renal function in larval zebrafish and demonstrate a decline in glomerular filtration rate after gentamicin exposure. The antineoplastic drug cisplatin, whose use in humans is limited by kidney toxicity, also causes typical histological changes and a decline in renal function in larval zebrafish. A specific inhibitor of Omi/HtrA2, a serine protease implicated in cisplatin-induced apoptosis, prevented renal failure and increased survival. This protective effect was confirmed in a mouse model of cisplatin-induced nephrotoxicity. Therefore, zebrafish provides a unique model system, amenable to genetic manipulation and drug screening, to explore the pathophysiology of ARF and establish novel therapies with potential use in mammals.

APA, Harvard, Vancouver, ISO, and other styles

24

Evensen, Lasse, Patrick L. Johansen, Gerbrand Koster, Kaizheng Zhu, Lars Herfindal, Martin Speth, Federico Fenaroli, et al. "Zebrafish as a model system for characterization of nanoparticles against cancer." Nanoscale 8, no. 2 (2016): 862–77. http://dx.doi.org/10.1039/c5nr07289a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Breitwieser, Helmut, Thomas Dickmeis, Marcel Vogt, Marco Ferg, and Christian Pylatiuk. "Fully Automated Pipetting Sorting System for Different Morphological Phenotypes of Zebrafish Embryos." SLAS TECHNOLOGY: Translating Life Sciences Innovation 23, no. 2 (December 8, 2017): 128–33. http://dx.doi.org/10.1177/2472630317745780.

Full text

Abstract:

Systems biology methods, such as transcriptomics and metabolomics, require large numbers of small model organisms, such as zebrafish embryos. Manual separation of mutant embryos from wild-type embryos is a tedious and time-consuming task that is prone to errors, especially if there are variable phenotypes of a mutant. Here we describe a zebrafish embryo sorting system with two cameras and image processing based on template-matching algorithms. In order to evaluate the system, zebrafish rx3 mutants that lack eyes due to a patterning defect in brain development were separated from their wild-type siblings. These mutants show glucocorticoid deficiency due to pituitary defects and serve as a model for human secondary adrenal insufficiencies. We show that the variable phenotypes of the mutant embryos can be safely distinguished from phenotypic wild-type zebrafish embryos and sorted from one petri dish into another petri dish or into a 96-well microtiter plate. On average, classification of a zebrafish embryo takes approximately 1 s, with a sensitivity and specificity of 87% to 95%, respectively. Other morphological phenotypes may be classified and sorted using similar techniques.

APA, Harvard, Vancouver, ISO, and other styles

26

McGonnell, I. M., and R. C. Fowkes. "Fishing for gene function – endocrine modelling in the zebrafish." Journal of Endocrinology 189, no. 3 (June 2006): 425–39. http://dx.doi.org/10.1677/joe.1.06683.

Full text

Abstract:

The use of zebrafish (Danio rerio) in scientific research is growing rapidly. It initially became popular as a model of vertebrate development because zebrafish embryos develop rapidly and are transparent. In the past 5 years, the sequencing of the zebrafish genome has increased the profile of zebrafish research even further, expanding into other areas such as pharmacology, cancer research and drug discovery. The use of zebrafish in endocrine research has mainly been confined to the study of the development of endocrine organs. However, it is likely to be a useful model in other areas of endocrinology, as there are a wide variety of both forward and reverse genetic techniques that can be employed in the zebrafish to decipher gene function in disease states. In this review, we compare the endocrine system of the zebrafish to mouse and human, demonstrating that the systems are sufficiently similar for zebrafish to be employed as a model for endocrine research. We subsequently review the repertoire of genetic techniques commonly employed in the zebrafish model to understand gene function in vertebrate development and disease. We anticipate that the use of these techniques will make the zebrafish a prominent model in endocrine research in the coming years.

APA, Harvard, Vancouver, ISO, and other styles

27

Lu, Bo, Misun Hwang, Cha Yong, and Luigi Moretti. "Zebrafish as a Model System to Screen Radiation Modifiers." Current Genomics 8, no. 6 (September 1, 2007): 360–69. http://dx.doi.org/10.2174/138920207783406497.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Ward, Alister, C. "The zebrafish as a model system for human disease." Frontiers in Bioscience 7, no. 1-3 (2002): d827. http://dx.doi.org/10.2741/ward.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Kikuchi, Kazu, Jennifer Holdway, Airon Wills, and Kenneth Poss. "S19-04 Cardiac regeneration in the zebrafish model system." Mechanisms of Development 126 (August 2009): S46. http://dx.doi.org/10.1016/j.mod.2009.06.1010.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Ijaz, Sundas, and Ellen J. Hoffman. "Zebrafish: A Translational Model System for Studying Neuropsychiatric Disorders." Journal of the American Academy of Child & Adolescent Psychiatry 55, no. 9 (September 2016): 746–48. http://dx.doi.org/10.1016/j.jaac.2016.06.008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Ward, Alister C. "The zebrafish as a model system for human disease." Frontiers in Bioscience 7, no. 4 (2002): d827–833. http://dx.doi.org/10.2741/a814.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Li, Qiaoli, Michael Frank, Christine I. Thisse, Bernard V. Thisse, and Jouni Uitto. "Zebrafish: A Model System to Study Heritable Skin Diseases." Journal of Investigative Dermatology 131, no. 3 (March 2011): 565–71. http://dx.doi.org/10.1038/jid.2010.388.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Bartoszewski, Slawomir, Mateusz Dawidziuk, Natalia Kasica, Roma Durak, Marta Jurek, Aleksandra Podwysocka, Dorothy Lys Guilbride, Piotr Podlasz, Cecilia Lanny Winata, and Pawel Gawlinski. "A Zebrafish/Drosophila Dual System Model for Investigating Human Microcephaly." Cells 11, no. 17 (September 1, 2022): 2727. http://dx.doi.org/10.3390/cells11172727.

Full text

Abstract:

Microcephaly presents in neurodevelopmental disorders with multiple aetiologies, including bi-allelic mutation in TUBGCP2, a component of the biologically fundamental and conserved microtubule-nucleation complex, γ-TuRC. Elucidating underlying principles driving microcephaly requires clear phenotype recapitulation and assay reproducibility, areas where go-to experimental models fall short. We present an alternative simple vertebrate/invertebrate dual system to investigate fundamental TUBGCP2-related processes driving human microcephaly and associated developmental traits. We show that antisense morpholino knockdown (KD) of the Danio rerio homolog, tubgcp2, recapitulates human TUBGCP2-associated microcephaly. Co-injection of wild type mRNA pre-empts microcephaly in 55% of KD zebrafish larvae, confirming causality. Body shortening observed in morphants is also rescued. Mitotic marker (pH3) staining further reveals aberrantly accumulated dividing brain cells in microcephalic tubgcp2 KD morphants, indicating that tubgcp2 depletion disrupts normal mitosis and/or proliferation in zebrafish neural progenitor brain cells. Drosophila melanogaster double knockouts (KO) for TUBGCP2 homologs Grip84/cg7716 also develop microcephalic brains with general microsomia. Exacerbated Grip84/cg7716-linked developmental aberration versus single mutations strongly suggests interactive or coinciding gene functions. We infer that tubgcp2 and Grip84/cg7716 affect brain size similarly to TUBGCP2 and recapitulate both microcephaly and microcephaly-associated developmental impact, validating the zebrafish/fly research model for human microcephaly. Given the conserved cross-phyla homolog function, the data also strongly support mitotic and/or proliferative disruption linked to aberrant microtubule nucleation in progenitor brain cells as key mechanistic defects for human microcephaly.

APA, Harvard, Vancouver, ISO, and other styles

34

Hoo, Jing Ying, Yatinesh Kumari, Mohd Farooq Shaikh, Seow Mun Hue, and Bey Hing Goh. "Zebrafish: A Versatile Animal Model for Fertility Research." BioMed Research International 2016 (2016): 1–20. http://dx.doi.org/10.1155/2016/9732780.

Full text

Abstract:

The utilization of zebrafish in biomedical research is very common in the research world nowadays. Today, it has emerged as a favored vertebrate organism for the research in science of reproduction. There is a significant growth in amount numbers of scientific literature pertaining to research discoveries in reproductive sciences in zebrafish. It has implied the importance of zebrafish in this particular field of research. In essence, the current available literature has covered from the very specific brain region or neurons of zebrafish, which are responsible for reproductive regulation, until the gonadal level of the animal. The discoveries and findings have proven that this small animal is sharing a very close/similar reproductive system with mammals. More interestingly, the behavioral characteristics and along with the establishment of animal courtship behavior categorization in zebrafish have laid an even stronger foundation and firmer reason on the suitability of zebrafish utilization in research of reproductive sciences. In view of the immense importance of this small animal for the development of reproductive sciences, this review aimed at compiling and describing the proximate close similarity of reproductive regulation on zebrafish and human along with factors contributing to the infertility, showing its versatility and its potential usage for fertility research.

APA, Harvard, Vancouver, ISO, and other styles

35

Spitsbergen, Jan M., and Michael L. Kent. "The State of the Art of the Zebrafish Model for Toxicology and Toxicologic Pathology Research—Advantages and Current Limitations." Toxicologic Pathology 31, no. 1_suppl (January 2003): 62–87. http://dx.doi.org/10.1080/01926230390174959.

Full text

Abstract:

The zebrafish (Danio rerio ) is now the pre-eminent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. The zebrafish genome will be completely sequenced within the next 1—2 years. Together with the substantial historical database regarding basic developmental biology, toxicology, and gene transfer, the rich foundation of molecular genetic and genomic data makes zebrafish a powerful model system for clarifying mechanisms in toxicity. In contrast to the highly advanced knowledge base on molecular developmental genetics in zebrafish, our database regarding infectious and noninfectious diseases and pathologic lesions in zebrafish lags far behind the information available on most other domestic mammalian and avian species, particularly rodents. Currently, minimal data are available regarding spontaneous neoplasm rates or spontaneous aging lesions in any of the commonly used wild-type or mutant lines of zebrafish. Therefore, to fully utilize the potential of zebrafish as an animal model for understanding human development, disease, and toxicology we must greatly advance our knowledge on zebrafish diseases and pathology.

APA, Harvard, Vancouver, ISO, and other styles

36

Usui, Takuji, Daniel W. A. Noble, Rose E. O’Dea, Melissa L. Fangmeier, Malgorzata Lagisz, Daniel Hesselson, and Shinichi Nakagawa. "The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio)." PeerJ 6 (January 17, 2018): e4292. http://dx.doi.org/10.7717/peerj.4292.

Full text

Abstract:

Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34–0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.

APA, Harvard, Vancouver, ISO, and other styles

37

van der Vaart, Michiel, Herman P. Spaink, and Annemarie H. Meijer. "Pathogen Recognition and Activation of the Innate Immune Response in Zebrafish." Advances in Hematology 2012 (2012): 1–19. http://dx.doi.org/10.1155/2012/159807.

Full text

Abstract:

The zebrafish has proven itself as an excellent model to study vertebrate innate immunity. It presents us with possibilities forin vivoimaging of host-pathogen interactions which are unparalleled in mammalian model systems. In addition, its suitability for genetic approaches is providing new insights on the mechanisms underlying the innate immune response. Here, we review the pattern recognition receptors that identify invading microbes, as well as the innate immune effector mechanisms that they activate in zebrafish embryos. We compare the current knowledge about these processes in mammalian models and zebrafish and discuss recent studies using zebrafish infection models that have advanced our general understanding of the innate immune system. Furthermore, we use transcriptome analysis of zebrafish infected withE. tarda, S. typhimurium, andM. marinumto visualize the gene expression profiles resulting from these infections. Our data illustrate that the two acute disease-causing pathogens,E. tardaandS. typhimurium, elicit a highly similar proinflammatory gene induction profile, while the chronic disease-causing pathogen,M. marinum, induces a weaker and delayed innate immune response.

APA, Harvard, Vancouver, ISO, and other styles

38

Adamson, Kathryn Isabel, Eamonn Sheridan, and Andrew James Grierson. "Use of zebrafish models to investigate rare human disease." Journal of Medical Genetics 55, no. 10 (July 31, 2018): 641–49. http://dx.doi.org/10.1136/jmedgenet-2018-105358.

Full text

Abstract:

Rare diseases are collectively common and often extremely debilitating. Following the emergence of next-generation sequencing (NGS) technologies, the variants underpinning rare genetic disorders are being unearthed at an accelerating rate. However, many rare conditions lack effective treatments due to their poorly understood pathophysiology. There is therefore a growing demand for the development of novel experimental models of rare genetic diseases, so that potentially causative variants can be validated, pathogenic mechanisms can be investigated and therapeutic targets can be identified. Animal models of rare diseases need to be genetically and physiologically similar to humans, and well-suited to large-scale experimental manipulation, considering the vast number of novel variants that are being identified through NGS. The zebrafish has emerged as a popular model system for investigating these variants, combining conserved vertebrate characteristics with a capacity for large-scale phenotypic and therapeutic screening. In this review, we aim to highlight the unique advantages of the zebrafish over other in vivo model systems for the large-scale study of rare genetic variants. We will also consider the generation of zebrafish disease models from a practical standpoint, by discussing how genome editing technologies, particularly the recently developed clustered regularly interspaced repeat (CRISPR)/CRISPR-associated protein 9 system, can be used to model rare pathogenic variants in zebrafish. Finally, we will review examples in the literature where zebrafish models have played a pivotal role in confirming variant causality and revealing the underlying mechanisms of rare diseases, often with wider implications for our understanding of human biology.

APA, Harvard, Vancouver, ISO, and other styles

39

Joshi, Bhagyashri, Ganesh Wagh, Harmandeep Kaur, and Chinmoy Patra. "Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology." Biology 10, no. 11 (November 13, 2021): 1177. http://dx.doi.org/10.3390/biology10111177.

Full text

Abstract:

Hypertension, a common chronic condition, may damage multiple organs, including the kidney, heart, and brain. Thus, it is essential to understand the pathology upon ectopic activation of the molecular pathways involved in mammalian hypertension to develop strategies to manage hypertension. Animal models play a crucial role in unraveling the disease pathophysiology by allowing incisive experimental procedures impossible in humans. Zebrafish, a small freshwater fish, have emerged as an important model system to study human diseases. The primary effector, Angiotensin II of the RAS pathway, regulates hemodynamic pressure overload mediated cardiovascular pathogenesis in mammals. There are various established mammalian models available to study pathophysiology in Angiotensin II-induced hypertension. Here, we have developed a zebrafish model to study pathogenesis by Angiotensin II. We find that intradermal Angiotensin II injection every 12 h can induce cardiac remodeling in seven days. We show that Angiotensin II injection in adult zebrafish causes cardiomyocyte hypertrophy and enhances cardiac cell proliferation. In addition, Angiotensin II induces ECM protein-coding gene expression and fibrosis in the cardiac ventricles. Thus, this study can conclude that Angiotensin II injection in zebrafish has similar implications as mammals, and zebrafish can be a model to study pathophysiology associated with AngII-RAS signaling.

APA, Harvard, Vancouver, ISO, and other styles

40

Sarmiento, Beatriz E., Santiago Callegari, Kemel A. Ghotme, and Veronica Akle. "Patient-Derived Xenotransplant of CNS Neoplasms in Zebrafish: A Systematic Review." Cells 11, no. 7 (April 2, 2022): 1204. http://dx.doi.org/10.3390/cells11071204.

Full text

Abstract:

Glioblastoma and neuroblastoma are the most common central nervous system malignant tumors in adult and pediatric populations. Both are associated with poor survival. These tumors are highly heterogeneous, having complex interactions among different cells within the tumor and with the tumor microenvironment. One of the main challenges in the neuro-oncology field is achieving optimal conditions to evaluate a tumor’s molecular genotype and phenotype. In this respect, the zebrafish biological model is becoming an excellent alternative for studying carcinogenic processes and discovering new treatments. This review aimed to describe the results of xenotransplantation of patient-derived CNS tumors in zebrafish models. The reviewed studies show that it is possible to maintain glioblastoma and neuroblastoma primary cell cultures and transplant the cells into zebrafish embryos. The zebrafish is a suitable biological model for understanding tumor progression and the effects of different treatments. This model offers new perspectives in providing personalized care and improving outcomes for patients living with central nervous system tumors.

APA, Harvard, Vancouver, ISO, and other styles

41

Ordas, Anita, Robert-Jan Raterink, Fraser Cunningham, Hans J. Jansen, Malgorzata I. Wiweger, Susanne Jong-Raadsen, Sabine Bos, et al. "Testing Tuberculosis Drug Efficacy in a Zebrafish High-Throughput Translational Medicine Screen." Antimicrobial Agents and Chemotherapy 59, no. 2 (November 10, 2014): 753–62. http://dx.doi.org/10.1128/aac.03588-14.

Full text

Abstract:

ABSTRACTThe translational value of zebrafish high-throughput screens can be improved when more knowledge is available on uptake characteristics of potential drugs. We investigated reference antibiotics and 15 preclinical compounds in a translational zebrafish-rodent screening system for tuberculosis. As a major advance, we have developed a new tool for testing drug uptake in the zebrafish model. This is important, because despite the many applications of assessing drug efficacy in zebrafish research, the current methods for measuring uptake using mass spectrometry do not take into account the possible adherence of drugs to the larval surface. Our approach combines nanoliter sampling from the yolk using a microneedle, followed by mass spectrometric analysis. To date, no single physicochemical property has been identified to accurately predict compound uptake; our method offers a great possibility to monitor how any novel compound behaves within the system. We have correlated the uptake data with high-throughput drug-screening data fromMycobacterium marinum-infected zebrafish larvae. As a result, we present an improved zebrafish larva drug-screening platform which offers new insights into drug efficacy and identifies potential false negatives and drugs that are effective in zebrafish and rodents. We demonstrate that this improved zebrafish drug-screening platform can complement conventional models ofin vivoMycobacterium tuberculosis-infected rodent assays. The detailed comparison of two vertebrate systems, fish and rodent, may give more predictive value for efficacy of drugs in humans.

APA, Harvard, Vancouver, ISO, and other styles

42

Biga, P. R., and F. W. Goetz. "Zebrafish and giant danio as models for muscle growth: determinate vs. indeterminate growth as determined by morphometric analysis." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 291, no. 5 (November 2006): R1327—R1337. http://dx.doi.org/10.1152/ajpregu.00905.2005.

Full text

Abstract:

The zebrafish has become an important genetic model, but their small size makes them impractical for traditional physiological studies. In contrast, the closely related giant danio is larger and can be utilized for physiological studies that can also make use of the extensive zebrafish genomic resources. In addition, the giant danio and zebrafish appear to exhibit different growth types, indicating the potential for developing a comparative muscle growth model system. Therefore, the present study was conducted to compare and characterize the muscle growth pattern of zebrafish and giant danio. Morphometric analyses demonstrated that giant danio exhibit an increased growth rate compared with zebrafish, starting as early as 2 wk posthatch. Total myotome area, mean fiber area, and total fiber number all exhibited positive correlations with larvae length in giant danio but not in zebrafish. Morphometric analysis of giant danio and zebrafish larvae demonstrated faster, more efficient growth in giant danio larvae. Similar to larger teleosts, adult giant danio exhibited increased growth rates in response to growth hormone, suggesting that giant danio exhibit indeterminate growth. In contrast, adult zebrafish do not exhibit mosaic hyperplasia, nor do they respond to growth hormone, suggesting they exhibit determinate growth like mammals. These results demonstrate that giant danio and zebrafish can be utilized as a direct comparative model system for muscle growth studies, with zebrafish serving as a model organism for determinate growth and giant danio for indeterminate growth.

APA, Harvard, Vancouver, ISO, and other styles

43

Tabassum, Nadia, Hongmei Tai, Da-Woon Jung, and Darren R. Williams. "Fishing for Nature’s Hits: Establishment of the Zebrafish as a Model for Screening Antidiabetic Natural Products." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–16. http://dx.doi.org/10.1155/2015/287847.

Full text

Abstract:

Diabetes mellitus affects millions of people worldwide and significantly impacts their quality of life. Moreover, life threatening diseases, such as myocardial infarction, blindness, and renal disorders, increase the morbidity rate associated with diabetes. Various natural products from medicinal plants have shown potential as antidiabetes agents in cell-based screening systems. However, many of these potential “hits” fail in mammalian tests, due to issues such as poor pharmacokinetics and/or toxic side effects. To address this problem, the zebrafish (Danio rerio) model has been developed as a “bridge” to provide an experimentally convenient animal-based screening system to identify drug candidates that are activein vivo. In this review, we discuss the application of zebrafish to drug screening technologies for diabetes research. Specifically, the discovery of natural product-based antidiabetes compounds using zebrafish will be described. For example, it has recently been demonstrated that antidiabetic natural compounds can be identified in zebrafish using activity guided fractionation of crude plant extracts. Moreover, the development of fluorescent-tagged glucose bioprobes has allowed the screening of natural product-based modulators of glucose homeostasis in zebrafish. We hope that the discussion of these advances will illustrate the value and simplicity of establishing zebrafish-based assays for antidiabetic compounds in natural products-based laboratories.

APA, Harvard, Vancouver, ISO, and other styles

44

LI, Li, and Ling-Fei LUO. "Zebrafish as the model system to study organogenesis and regeneration." Hereditas (Beijing) 35, no. 4 (September 27, 2013): 421–32. http://dx.doi.org/10.3724/sp.j.1005.2013.00421.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Korzh, V., I. Kondrychyn, and C. Winata. "The Zebrafish as a New Model System for Experimental Biology." Cytology and Genetics 52, no. 6 (November 2018): 406–15. http://dx.doi.org/10.3103/s009545271806004x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Steele, Shelby L., Sergey V. Prykhozhij, and Jason N. Berman. "Zebrafish as a model system for mitochondrial biology and diseases." Translational Research 163, no. 2 (February 2014): 79–98. http://dx.doi.org/10.1016/j.trsl.2013.08.008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

FADOOL, J., and J. DOWLING. "Zebrafish: A model system for the study of eye genetics." Progress in Retinal and Eye Research 27, no. 1 (January 2008): 89–110. http://dx.doi.org/10.1016/j.preteyeres.2007.08.002.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Dooley, K. "Zebrafish: a model system for the study of human disease." Current Opinion in Genetics & Development 10, no. 3 (June 1, 2000): 252–56. http://dx.doi.org/10.1016/s0959-437x(00)00074-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Schaaf, M. J. M., A. Chatzopoulou, and H. P. Spaink. "The zebrafish as a model system for glucocorticoid receptor research." Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 153, no. 1 (May 2009): 75–82. http://dx.doi.org/10.1016/j.cbpa.2008.12.014.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Marí-Beffa, Manuel, Jesús A. Santamaría, Carmen Murciano, Leonor Santos-Ruiz, José A. Andrades, Enrique Guerado, and José Becerra. "Zebrafish Fins as a Model System for Skeletal Human Studies." Scientific World JOURNAL 7 (2007): 1114–27. http://dx.doi.org/10.1100/tsw.2007.190.

Full text

Abstract:

Recent studies on the morphogenesis of the fins ofDanio rerio(zebrafish) during development and regeneration suggest that a number of inductive signals involved in the process are similar to some of those that affect bone and cartilage differentiation in mammals and humans. Akimenko et al. (2002) has shown that bone morphogenetic protein-2b (BMP2b) is involved in the induction of dermal bone differentiation during fin regeneration. Many other groups have also shown that molecules from the transforming growth factor-beta superfamily (TGFβ), including BMP2, are effective in promoting chondrogenesis and osteogenesisin vivoin higher vertebrates, including humans. In the present study, we review the state of the art of this topic by a comparative analysis of skeletal tissue development, regeneration and renewal processes in tetrapods, and fin regeneration in fishes. A general conclusion of this study states that lepidotrichia is a special skeletal tissue different to cartilage, bone, enamel, or dentine in fishes, according to its extracellular matrix (ECM) composition. However, the empirical analysis of inducing signals of skeletal tissues in fishes and tetrapods suggests that lepidotrichia is different to any responding features with main skeletal tissues. A number of new inductive molecules are arising from fin development and regeneration studies that might establish an empirical basis for further molecular approaches to mammal skeletal tissues differentiation. Despite the tissue dissimilarity, this empirical evidence might finally lead to clinical applications to skeletal disorders in humans.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Zebrafish model system'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles