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Journal articles on the topic 'Yeast two-hybrid'

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Published: 4 June 2021

Last updated: 6 July 2024

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1

NISHIHARA, Tsutomu, and Jun-ichi NISHIKAWA. "Bioassay for endocrine disruptors by using yeast two-hybrid system." Folia Pharmacologica Japonica 118, no. 3 (2001): 203–10. http://dx.doi.org/10.1254/fpj.118.203.

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2

Hughes-Davies, L. "The Yeast Two-Hybrid System." Journal of Medical Genetics 35, no. 8 (August 1, 1998): 704. http://dx.doi.org/10.1136/jmg.35.8.704.

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3

Szeberényi, József. "The yeast two-hybrid system." Biochemistry and Molecular Biology Education 34, no. 4 (July 2006): 306–7. http://dx.doi.org/10.1002/bmb.2006.494034042638.

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4

NAKAMURA, HIDEMITSU. "Diversification of yeast two-hybrid system." Kagaku To Seibutsu 37, no. 4 (1999): 249–50. http://dx.doi.org/10.1271/kagakutoseibutsu1962.37.249.

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5

Koegl, M., and P. Uetz. "Improving yeast two-hybrid screening systems." Briefings in Functional Genomics and Proteomics 6, no. 4 (January 22, 2008): 302–12. http://dx.doi.org/10.1093/bfgp/elm035.

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6

Reece-Hoyes, John S., and Albertha J. M. Walhout. "Generating Yeast Two-Hybrid Bait Strains." Cold Spring Harbor Protocols 2018, no. 7 (July 2018): pdb.prot094979. http://dx.doi.org/10.1101/pdb.prot094979.

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7

Reece-Hoyes, John S., and Albertha J. M. Walhout. "Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays." Cold Spring Harbor Protocols 2018, no. 7 (July 2018): pdb.top094953. http://dx.doi.org/10.1101/pdb.top094953.

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8

Serebriiskii, Ilya G., Rui Fang, Ekaterina Latypova, Richard Hopkins, Charles Vinson, J. Keith Joung, and Erica A. Golemis. "A Combined Yeast/Bacteria Two-hybrid System." Molecular & Cellular Proteomics 4, no. 6 (March 20, 2005): 819–26. http://dx.doi.org/10.1074/mcp.t500005-mcp200.

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9

Van Criekinge, Wim, and Rudi Beyaert. "Yeast two-hybrid: State of the art." Biological Procedures Online 2, no. 1 (October 1999): 1–38. http://dx.doi.org/10.1251/bpo16.

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10

Uetz, Peter. "Editorial for “The Yeast two-hybrid system”." Methods 58, no. 4 (December 2012): 315–16. http://dx.doi.org/10.1016/j.ymeth.2013.01.001.

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11

Lawit, Shai J., Kevin O’Grady, William B. Gurley, and Eva Czarnecka-Verner. "Yeast two-hybrid map of Arabidopsis TFIID." Plant Molecular Biology 64, no. 1-2 (March 6, 2007): 73–87. http://dx.doi.org/10.1007/s11103-007-9135-1.

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12

Parrish, Jodi R., Keith D. Gulyas, and Russell L. Finley. "Yeast two-hybrid contributions to interactome mapping." Current Opinion in Biotechnology 17, no. 4 (August 2006): 387–93. http://dx.doi.org/10.1016/j.copbio.2006.06.006.

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13

McAlister-Henn, Lee, Natalie Gibson, and Ellen Panisko. "Applications of the Yeast Two-Hybrid System." Methods 19, no. 2 (October 1999): 330–37. http://dx.doi.org/10.1006/meth.1999.0860.

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14

Eustace, Rosanne, and R. J. Thornton. "Selective hybridization of wine yeasts for higher yields of glycerol." Canadian Journal of Microbiology 33, no. 2 (February 1, 1987): 112–17. http://dx.doi.org/10.1139/m87-019.

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Wines that are lacking in body may be improved by the presence of greater amounts of glycerol. Wine yeast strains vary in their ability to produce glycerol. A programme of hybridizing yeast strains while selecting for increased production of glycerol was undertaken. Three generations of hybridization resulted in yeast strains which produced 10–11 g glycerol/L compared with 3.0–6.6 g/L produced by the wine yeast strains of the original breeding stock. Industrial-scale winemaking confirmed the ability of two hybrid strains to produce similar amounts of glycerol to those observed in laboratory-scale fermentations. The activity of the enzyme glycerol-3-phosphate dehydrogenase was measured in crude extracts of two breeding stock wine yeasts and in two final generation hybrid strains. The observed activities were lower in the products of the hybridization programme than in the original wine yeast strains. Two alternative explanations are suggested. (i) Selective hybridization may select for the alleles of the gene which codes for an alcohol dehydrogenase I isozyme which has a lower activity resulting in increased glycerol production. (ii) Phospholipid synthesis is reduced and the glycerol-3-phosphate, which is the major precursor of phospholipids in yeasts, accumulates as glycerol.

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15

Luo, Ying, Anna Batalao, Helen Zhou, and Li Zhu. "Mammalian Two-Hybrid System: A Complementary Approach to the Yeast Two-Hybrid System." BioTechniques 22, no. 2 (February 1997): 350–52. http://dx.doi.org/10.2144/97222pf02.

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16

Saito, Youhei, Takanobu Nakagawa, Ayana Kakihana, Yoshia Nakamura, Tomomi Nabika, Michihiro Kasai, Mai Takamori, et al. "Yeast Two-Hybrid and One-Hybrid Screenings Identify Regulators ofhsp70Gene Expression." Journal of Cellular Biochemistry 117, no. 9 (March 10, 2016): 2109–17. http://dx.doi.org/10.1002/jcb.25517.

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17

IWATA, Nobuhisa. "Molecular Cloning Using the Yeast Two-hybrid System." Japanese Journal of Thrombosis and Hemostasis 9, no. 2 (1998): 126–32. http://dx.doi.org/10.2491/jjsth.9.126.

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18

Chen, Yu-Chi, Seesandra Venkatappa Rajagopala, Thorsten Stellberger, and Peter Uetz. "Exhaustive benchmarking of the yeast two-hybrid system." Nature Methods 7, no. 9 (September 2010): 667–68. http://dx.doi.org/10.1038/nmeth0910-667.

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19

Parchaliuk, Debra L., Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, and R. Daniel Gietz. "Yeast two-hybrid system: part A: screen preparation." Technical Tips Online 4, no. 1 (January 1999): 6–15. http://dx.doi.org/10.1016/s1366-2120(08)70129-6.

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20

Parchaliuk, Debra L., Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, and R. Daniel Gietz. "Yeast two-hybrid system: part B—screening procedure." Technical Tips Online 4, no. 1 (January 1999): 30–34. http://dx.doi.org/10.1016/s1366-2120(08)70133-8.

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21

Parchaliuk, Debra L., Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, and R. Daniel Gietz. "Yeast two-hybrid system: part C—characterizing positives." Technical Tips Online 4, no. 1 (January 1999): 35–44. http://dx.doi.org/10.1016/s1366-2120(08)70134-x.

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22

Hollingsworth, Robert, and Julia H. White. "Target discovery using the yeast two-hybrid system." Drug Discovery Today: TARGETS 3, no. 3 (June 2004): 97–103. http://dx.doi.org/10.1016/s1741-8372(04)02414-4.

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23

Lin, Guiting, Zhiwen Zhang, Tong Zang, Dianqi Xin, Zhijie Chang, and Yinglu Guo. "Interaction of hTCF4 by yeast two-hybrid system." Chinese Science Bulletin 45, no. 21 (November 2000): 1973–77. http://dx.doi.org/10.1007/bf02909690.

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24

White, M. A. "The yeast two-hybrid system: forward and reverse." Proceedings of the National Academy of Sciences 93, no. 19 (September 17, 1996): 10001–3. http://dx.doi.org/10.1073/pnas.93.19.10001.

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25

Lecrenier, Nicolas, Françoise Foury, and Andre Goffeau. "Two-hybrid systematic screening of the yeast proteome." BioEssays 20, no. 1 (December 6, 1998): 1–5. http://dx.doi.org/10.1002/(sici)1521-1878(199801)20:1<1::aid-bies2>3.0.co;2-y.

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26

Paiano, Aurora, Azzurra Margiotta, Maria De Luca, and Cecilia Bucci. "Yeast Two-Hybrid Assay to Identify Interacting Proteins." Current Protocols in Protein Science 95, no. 1 (August 21, 2018): e70. http://dx.doi.org/10.1002/cpps.70.

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27

Wendland, Jürgen. "Lager Yeast Comes of Age." Eukaryotic Cell 13, no. 10 (August 1, 2014): 1256–65. http://dx.doi.org/10.1128/ec.00134-14.

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ABSTRACTAlcoholic fermentations have accompanied human civilizations throughout our history. Lager yeasts have a several-century-long tradition of providing fresh beer with clean taste. The yeast strains used for lager beer fermentation have long been recognized as hybrids between twoSaccharomycesspecies. We summarize the initial findings on this hybrid nature, the genomics/transcriptomics of lager yeasts, and established targets of strain improvements. Next-generation sequencing has provided fast access to yeast genomes. Its use in population genomics has uncovered many more hybridization events withinSaccharomycesspecies, so that lager yeast hybrids are no longer the exception from the rule. These findings have led us to propose network evolution withinSaccharomycesspecies. This “web of life” recognizes the ability of closely related species to exchange DNA and thus drain from a combined gene pool rather than be limited to a gene pool restricted by speciation. Within the domesticated lager yeasts, two groups, the Saaz and Frohberg groups, can be distinguished based on fermentation characteristics. Recent evidence suggests that these groups share an evolutionary history. We thus propose to refer to the Saaz group asSaccharomyces carlsbergensisand to the Frohberg group asSaccharomyces pastorianusbased on their distinct genomes. New insight into the hybrid nature of lager yeast will provide novel directions for future strain improvement.

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28

Shaghaghi-Moghaddam, Reza, Hoda Jafarizadeh-Malmiri, Parviz Mehdikhani, Sepide Jalalian, and Reza Alijanianzadeh. "Screening of the five different wild, traditional and industrial Saccharomyces cerevisiae strains to overproduce bioethanol in the batch submerged fermentation." Zeitschrift für Naturforschung C 73, no. 9-10 (September 25, 2018): 361–66. http://dx.doi.org/10.1515/znc-2017-0180.

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Abstract Efforts to produce bioethanol with higher productivity in a batch submerged fermentation were made by evaluating the bioethanol production of the five different strains of Saccharomyces cerevisiae, namely, NCYC 4109 (traditional bakery yeast), SFO6 (industrial yeast), TTCC 2956 (hybrid baking yeast) and two wild yeasts, PTCC 5052 and BY 4743. The bioethanol productivity and kinetic parameters for all five yeasts at constant fermentation conditions, during 72 h, were evaluated and monitored. The obtained results indicated that compared to the wild yeasts, both traditional bakery (NCYC 4109) and industrial (SFO6) yeasts had higher bioethanol productivity (0.9 g/L h). Significant (p<0.05) differences between biomass concentration of NCYC 4109 yeast and those of other yeasts 30 h after start of fermentation, and its high bioethanol concentration (59.19 g/L) and yield over consumed sugars (77.25%) were highlighted among all the studied yeasts. Minimum bioethanol productivity was obtained using yeasts PTCC 5052 (0.7 g/L h) and TTCC 2956 (0.86 g/L h). However, maximum yield over consumed sugar was obtained using the yeast TTCC 2956 (79.41%).

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29

Walhout, Albertha J. M., Simon J. Boulton, and Marc Vidal. "Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm." Yeast 1, no. 2 (January 1, 2000): 88–94. http://dx.doi.org/10.1155/2000/156745.

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The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as ‘functional genomics’. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein–protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein–protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans.

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30

Walhout, Albertha J. M., Simon J. Boulton, and Marc Vidal. "Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm." Yeast 1, no. 2 (2000): 88–94. http://dx.doi.org/10.1002/1097-0061(20000630)17:2<88::aid-yea20>3.0.co;2-y.

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The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as ‘functional genomics’. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein–protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein–protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeastSaccharomyces cerevisiaeand the nematodeCaenorhabditis elegans.

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31

PAN, Xiao. "DETECTION OF MICROCYSTINS BASED ON YEAST TWO-HYBRID SYSTEM." Acta Hydrobiologica Sinica 32, no. 2 (November 20, 2008): 201–6. http://dx.doi.org/10.3724/sp.j.1035.2008.00201.

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32

Brückner, Anna, Cécile Polge, Nicolas Lentze, Daniel Auerbach, and Uwe Schlattner. "Yeast Two-Hybrid, a Powerful Tool for Systems Biology." International Journal of Molecular Sciences 10, no. 6 (June 18, 2009): 2763–88. http://dx.doi.org/10.3390/ijms10062763.

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33

Liu, Ying, Zabeena Merchant, Hao-Ching Hsiao, Kim L. Gonzalez, Kathleen S. Matthews, and Sarah E. Bondos. "Media composition influences yeast one- and two-hybrid results." Biological Procedures Online 13, no. 1 (2011): 6. http://dx.doi.org/10.1186/1480-9222-13-6.

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34

Liu, Y., N. T. Woods, D. Kim, M. Sweet, A. N. A. Monteiro, and R. Karchin. "Yeast two-hybrid junk sequences contain selected linear motifs." Nucleic Acids Research 39, no. 19 (July 23, 2011): e128-e128. http://dx.doi.org/10.1093/nar/gkr600.

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35

Vidalain, Pierre-Olivier, Mike Boxem, Hui Ge, Siming Li, and Marc Vidal. "Increasing specificity in high-throughput yeast two-hybrid experiments." Methods 32, no. 4 (April 2004): 363–70. http://dx.doi.org/10.1016/j.ymeth.2003.10.001.

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36

Rain, Jean-Christophe, Alexandra Cribier, Annabelle Gérard, Stéphane Emiliani, and Richard Benarous. "Yeast two-hybrid detection of integrase–host factor interactions." Methods 47, no. 4 (April 2009): 291–97. http://dx.doi.org/10.1016/j.ymeth.2009.02.002.

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37

Rajagopala, Seesandra V., Patricia Sikorski, J. Harry Caufield, Andrey Tovchigrechko, and Peter Uetz. "Studying protein complexes by the yeast two-hybrid system." Methods 58, no. 4 (December 2012): 392–99. http://dx.doi.org/10.1016/j.ymeth.2012.07.015.

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38

Caufield, J. H., Neha Sakhawalkar, and Peter Uetz. "A comparison and optimization of yeast two-hybrid systems." Methods 58, no. 4 (December 2012): 317–24. http://dx.doi.org/10.1016/j.ymeth.2012.12.001.

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39

Xin, Xiangbo, Ting Wang, Xinfeng Liu, Guoning Sui, Congfei Jin, Yingwei Yue, Shuping Yang, and Hong Guo. "A yeast two-hybrid assay reveals CMYA1 interacting proteins." Comptes Rendus Biologies 340, no. 6-7 (June 2017): 314–23. http://dx.doi.org/10.1016/j.crvi.2017.06.003.

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40

Cao, Yang, Usha Nair, Kyoko Yasumura-Yorimitsu, and Daniel J. Klionsky. "A multipleATGgene knockout strain for yeast two-hybrid analysis." Autophagy 5, no. 5 (July 2009): 699–705. http://dx.doi.org/10.4161/auto.5.5.8382.

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41

Konopka, James. "The Yeast Two-Hybrid System.Paul L. Bartel , Stanley Fields." Quarterly Review of Biology 73, no. 4 (December 1998): 493. http://dx.doi.org/10.1086/420439.

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42

Mehla, Jitender, J. Harry Caufield, and Peter Uetz. "Mapping Protein–Protein Interactions Using Yeast Two-Hybrid Assays." Cold Spring Harbor Protocols 2015, no. 5 (May 2015): pdb.prot086157. http://dx.doi.org/10.1101/pdb.prot086157.

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43

Bhartur, Sheela G., and James R. Goldenring. "Mapping of Ezrin Dimerization Using Yeast Two-Hybrid Screening." Biochemical and Biophysical Research Communications 243, no. 3 (February 1998): 874–77. http://dx.doi.org/10.1006/bbrc.1998.8196.

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44

Aho, Sirpa, Airi Arffman, Tiina Pummi, and Jouni Uitto. "A Novel Reporter GeneMEL1for the Yeast Two-Hybrid System." Analytical Biochemistry 253, no. 2 (November 1997): 270–72. http://dx.doi.org/10.1006/abio.1997.2394.

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45

Helmuth, Martin, Wilko Altrock, Tobias M. Böckers, Eckart D. Gundelfinger, and Michael R. Kreutz. "An Electrotransfection Protocol for Yeast Two-Hybrid Library Screening." Analytical Biochemistry 293, no. 1 (June 2001): 149–52. http://dx.doi.org/10.1006/abio.2001.5107.

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46

PAN, Xiao, Ping-Ping SHEN, and Zi-Chun HUA. "DETECTION OF MICROCYSTINS BASED ON YEAST TWO-HYBRID SYSTEM." Acta Hydrobiologica Sinica 32, no. 2 (March 1, 2008): 201–6. http://dx.doi.org/10.3724/issn1000-3207-2008-2-201-q.

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47

Salinas, Paloma, Sirine Bibak, Raquel Cantos, Lorena Tremiño, Carmen Jerez, Trinidad Mata-Balaguer, and Asunción Contreras. "Studies on the PII-PipX-NtcA Regulatory Axis of Cyanobacteria Provide Novel Insights into the Advantages and Limitations of Two-Hybrid Systems for Protein Interactions." International Journal of Molecular Sciences 25, no. 10 (May 16, 2024): 5429. http://dx.doi.org/10.3390/ijms25105429.

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Yeast two-hybrid approaches, which are based on fusion proteins that must co-localise to the nucleus to reconstitute the transcriptional activity of GAL4, have greatly contributed to our understanding of the nitrogen interaction network of cyanobacteria, the main hubs of which are the trimeric PII and the monomeric PipX regulators. The bacterial two-hybrid system, based on the reconstitution in the E. coli cytoplasm of the adenylate cyclase of Bordetella pertussis, should provide a relatively faster and presumably more physiological assay for cyanobacterial proteins than the yeast system. Here, we used the bacterial two-hybrid system to gain additional insights into the cyanobacterial PipX interaction network while simultaneously assessing the advantages and limitations of the two most popular two-hybrid systems. A comprehensive mutational analysis of PipX and bacterial two-hybrid assays were performed to compare the outcomes between yeast and bacterial systems. We detected interactions that were previously recorded in the yeast two-hybrid system as negative, as well as a “false positive”, the self-interaction of PipX, which is rather an indirect interaction that is dependent on PII homologues from the E. coli host, a result confirmed by Western blot analysis with relevant PipX variants. This is, to our knowledge, the first report of the molecular basis of a false positive in the bacterial two-hybrid system.

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48

Usher, Jane, and Ursula Bond. "Recombination between Homoeologous Chromosomes of Lager Yeasts Leads to Loss of Function of the Hybrid GPH1 Gene." Applied and Environmental Microbiology 75, no. 13 (May 8, 2009): 4573–79. http://dx.doi.org/10.1128/aem.00351-09.

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ABSTRACT Yeasts used in the production of lagers contain complex allopolyploid genomes, resulting from the fusion of two different yeast species closely related to Saccharomyces cerevisiae and Saccharomyces bayanus. Recombination between the homoeologous chromosomes has generated a number of hybrid chromosomes. These recombination events provide potential for adaptive evolution through the loss or gain of gene function. We have examined the genotypic and phenotypic effects of one of the conserved recombination events that occurred on chromosome XVI in the region of YPR159W and YPR160W. Our analysis shows that the recombination event occurred within the YPR160W gene, which encodes the enzyme glycogen phosphorylase and generates a hybrid gene that does not produce mature mRNA and is nonfunctional due to frameshifts in the coding region. The loss of function of the hybrid gene leads to glycogen levels similar to those found in haploid yeast strains. The implications for the control of glycogen levels in fermentative yeasts are discussed.

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49

Chen, Qiu-Min, Na Cui, Yang Yu, Xiang-Nan Meng, and Hai-Yan Fan. "Expression and Construction of Yeast Expression Vector Containing CsTCTP1 Gene from Cucumber in Yeast Two-Hybrid System." Open Plant Science Journal 10, no. 1 (June 30, 2017): 55–61. http://dx.doi.org/10.2174/1874294701710010055.

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Background: The translationally controlled tumor protein (TCTP) was originally found in tumor tissue, and later found in other tissues. Initially, TCTP was considered a kind of growth-associated protein. Recent studies have shown that TCTP has many biological functions. Objective: To verification of CsTCTP1 gene function by yeast two-hybrid system, the pGBKT7- CsTCTP1 yeast expression vector was constructed and cytotoxicity and self-activating activity were detected, which could lay the foundation for further studies on gene function and make a preparation for verification of CsTCTP1 gene function by yeast two-hybrid system. Method: Specific PCR, conventional sequencing, heat shock conversion method and TE/LiAC transformation method. Results: We constructed a yeast expression vector containing the CsTCTP1 gene. The CsTCTP1 coding sequence was inserted into a pGBKT7 vector as a bait protein and then transformed into the Y2HGold yeast stain. Conclusion: We found that CsTCTP1 protein had no cytotoxic effect and could not be self-activated. The constructed bait expression vector can be used in the subsequent yeast two - hybrid detection system.

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50

Lin, Lin, Yuhong Shi, Zhaopeng Luo, Yuwen Lu, Hongying Zheng, Fei Yan, Jiong Chen, Jianping Chen, M. J. Adams, and Yunfeng Wu. "Protein–protein interactions in two potyviruses using the yeast two-hybrid system." Virus Research 142, no. 1-2 (June 2009): 36–40. http://dx.doi.org/10.1016/j.virusres.2009.01.006.

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