Academic literature on the topic 'Yeast chromosome'

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Journal articles on the topic "Yeast chromosome"

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Ross, L. O., D. Treco, A. Nicolas, J. W. Szostak, and D. Dawson. "Meiotic recombination on artificial chromosomes in yeast." Genetics 131, no. 3 (July 1, 1992): 541–50. http://dx.doi.org/10.1093/genetics/131.3.541.

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Abstract We have examined the meiotic recombination characteristics of artificial chromosomes in Saccharomyces cerevisiae. Our experiments were carried out using minichromosome derivatives of yeast chromosome III and yeast artificial chromosomes composed primarily of bacteriophage lambda DNA. Tetrad analysis revealed that the artificial chromosomes exhibit very low levels of meiotic recombination. However, when a 12.5-kbp fragment from yeast chromosome VIII was inserted into the right arm of the artificial chromosome, recombination within that arm mimicked the recombination characteristics of the fragment in its natural context including the ability of crossovers to ensure meiotic disjunction. Both crossing over and gene conversion (within the ARG4 gene contained within the fragment) were measured in the experiments. Similarly, a 55-kbp region from chromosome III carried on a minichromosome showed crossover behavior indistinguishable from that seen when it is carried on chromosome III. We discuss the notion that, in yeast, meiotic recombination behavior is determined locally by small chromosomal regions that function free of the influence of the chromosome as a whole.
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Bystricky, Kerstin, Thierry Laroche, Griet van Houwe, Marek Blaszczyk, and Susan M. Gasser. "Chromosome looping in yeast." Journal of Cell Biology 168, no. 3 (January 31, 2005): 375–87. http://dx.doi.org/10.1083/jcb.200409091.

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Long-range chromosome organization is known to influence nuclear function. Budding yeast centromeres cluster near the spindle pole body, whereas telomeres are grouped in five to eight perinuclear foci. Using live microscopy, we examine the relative positions of right and left telomeres of several yeast chromosomes. Integrated lac and tet operator arrays are visualized by their respective repressor fused to CFP and YFP in interphase yeast cells. The two ends of chromosomes 3 and 6 interact significantly but transiently, forming whole chromosome loops. For chromosomes 5 and 14, end-to-end interaction is less frequent, yet telomeres are closer to each other than to the centromere, suggesting that yeast chromosomes fold in a Rabl-like conformation. Disruption of telomere anchoring by deletions of YKU70 or SIR4 significantly compromises contact between two linked telomeres. These mutations do not, however, eliminate coordinated movement of telomere (Tel) 6R and Tel6L, which we propose stems from the territorial organization of yeast chromosomes.
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Chen, Rey-Huei. "Chromosome detachment from the nuclear envelope is required for genomic stability in closed mitosis." Molecular Biology of the Cell 30, no. 13 (June 15, 2019): 1578–86. http://dx.doi.org/10.1091/mbc.e19-02-0098.

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Mitosis in metazoans involves detachment of chromosomes from the nuclear envelope (NE) and NE breakdown, whereas yeasts maintain the nuclear structure throughout mitosis. It remains unknown how chromosome attachment to the NE might affect chromosome movement in yeast. By using a rapamycin-induced dimerization system to tether a specific locus of the chromosome to the NE, I found that the tethering delays the separation and causes missegregation of the region distal to the tethered site. The phenotypes are exacerbated by mutations in kinetochore components and Aurora B kinase Ipl1. The chromosome region proximal to the centromere is less affected by the tether, but it exhibits excessive oscillation before segregation. Furthermore, the tether impacts full extension of the mitotic spindle, causing abrupt shrinkage or bending of the spindle in shortened anaphase. The study supports detachment of chromosomes from the NE being required for faithful chromosome segregation in yeast and segregation of tethered chromosomes being dependent on a fully functional mitotic apparatus.
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Guacci, V., and D. B. Kaback. "Distributive disjunction of authentic chromosomes in Saccharomyces cerevisiae." Genetics 127, no. 3 (March 1, 1991): 475–88. http://dx.doi.org/10.1093/genetics/127.3.475.

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Abstract Distributive disjunction is defined as the first division meiotic segregation of either nonhomologous chromosomes that lack homologs or homologous chromosomes that have not recombined. To determine if chromosomes from the yeast Saccharomyces cerevisiae were capable of distributive disjunction, we constructed a strain that was monosomic for both chromosome I and chromosome III and analyzed the meiotic segregation of the two monosomic chromosomes. In addition, we bisected chromosome I into two functional chromosome fragments, constructed strains that were monosomic for both chromosome fragments and examined meiotic segregation of the chromosome fragments in the monosomic strains. The two nonhomologous chromosomes or chromosome fragments appeared to segregate from each other in approximately 90% of the asci analyzed, indicating that yeast chromosomes were capable of distributive disjunction. We also examined the ability of a small nonhomologous centromere containing plasmid to participate in distributive disjunction with the two nonhomologous monosomic chromosomes. The plasmid appeared to efficiently participate with the two full length chromosomes suggesting that distributive disjunction in yeast is not dependent on chromosome size. Thus, distributive disjunction in S. cerevisiae appears to be different from Drosophila melanogaster where a different sized chromosome is excluded from distributive disjunction when two similar size nonhomologous chromosomes are present.
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Albert, Benjamin, Julien Mathon, Ashutosh Shukla, Hicham Saad, Christophe Normand, Isabelle Léger-Silvestre, David Villa, et al. "Systematic characterization of the conformation and dynamics of budding yeast chromosome XII." Journal of Cell Biology 202, no. 2 (July 22, 2013): 201–10. http://dx.doi.org/10.1083/jcb.201208186.

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Chromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ∼100 kb along the chromosome, and investigated their motion over broad time scales (0.2–400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion.
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Fuchs, Jörg, Alexander Lorenz, and Josef Loidl. "Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes." Journal of Cell Science 115, no. 6 (March 15, 2002): 1213–20. http://dx.doi.org/10.1242/jcs.115.6.1213.

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The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, as chromosomal loci with tandem repeats of exogenous tetO sequences are frequently associated. These associations occur only if TetR molecules are present. tetO tandem repeats associate regardless of their chromosomal context. When they are present at a proximal and a distal chromosomal position, they perturb the normal polarized Rabl-arrangement of chromosome arms by recruiting chromosome ends to the centromeric pole of the nucleus. Associations are established at G1 and are reduced during S-phase and mitosis. This system may serve as a model for the role of DNA sequence-specific binding proteins in imposing nonrandom distribution of chromosomes within the nucleus.
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Spell, R. M., and C. Holm. "Nature and distribution of chromosomal intertwinings in Saccharomyces cerevisiae." Molecular and Cellular Biology 14, no. 2 (February 1994): 1465–76. http://dx.doi.org/10.1128/mcb.14.2.1465-1476.1994.

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To elucidate yeast chromosome structure and behavior, we examined the breakage of entangled chromosomes in DNA topoisomerase II mutants by hybridization to chromosomal DNA resolved by pulsed-field gel electrophoresis. Our study reveals that large and small chromosomes differ in the nature and distribution of their intertwinings. Probes to large chromosomes (450 kb or larger) detect chromosome breakage, but probes to small chromosomes (380 kb or smaller) reveal no breakage products. Examination of chromosomes with one small arm and one large arm suggests that the two arms behave independently. The acrocentric chromosome XIV breaks only on the long arm, and its preferred region of breakage is approximately 200 kb from the centromere. When the centromere of chromosome XIV is relocated, the preferred region of breakage shifts accordingly. These results suggest that large chromosomes break because they have long arms and small chromosomes do not break because they have small arms. Indeed, a small metacentric chromosome can be made to break if it is rearranged to form a telocentric chromosome with one long arm or a ring with an "infinitely" long arm. These results suggest a model of chromosomal intertwining in which the length of the chromosome arm prevents intertwinings from passively resolving off the end of the arm during chromosome segregation.
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Spell, R. M., and C. Holm. "Nature and distribution of chromosomal intertwinings in Saccharomyces cerevisiae." Molecular and Cellular Biology 14, no. 2 (February 1994): 1465–76. http://dx.doi.org/10.1128/mcb.14.2.1465.

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To elucidate yeast chromosome structure and behavior, we examined the breakage of entangled chromosomes in DNA topoisomerase II mutants by hybridization to chromosomal DNA resolved by pulsed-field gel electrophoresis. Our study reveals that large and small chromosomes differ in the nature and distribution of their intertwinings. Probes to large chromosomes (450 kb or larger) detect chromosome breakage, but probes to small chromosomes (380 kb or smaller) reveal no breakage products. Examination of chromosomes with one small arm and one large arm suggests that the two arms behave independently. The acrocentric chromosome XIV breaks only on the long arm, and its preferred region of breakage is approximately 200 kb from the centromere. When the centromere of chromosome XIV is relocated, the preferred region of breakage shifts accordingly. These results suggest that large chromosomes break because they have long arms and small chromosomes do not break because they have small arms. Indeed, a small metacentric chromosome can be made to break if it is rearranged to form a telocentric chromosome with one long arm or a ring with an "infinitely" long arm. These results suggest a model of chromosomal intertwining in which the length of the chromosome arm prevents intertwinings from passively resolving off the end of the arm during chromosome segregation.
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McManus, J., P. Perry, A. T. Sumner, D. M. Wright, E. J. Thomson, R. C. Allshire, N. D. Hastie, and W. A. Bickmore. "Unusual chromosome structure of fission yeast DNA in mouse cells." Journal of Cell Science 107, no. 3 (March 1, 1994): 469–86. http://dx.doi.org/10.1242/jcs.107.3.469.

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Chromosomes from the fission yeast Schizosaccharomyces pombe have been introduced into mouse cells by protoplast fusion. In most cell lines the yeast DNA integrates into a single site within a mouse chromosome and results in striking chromosome morphology at metaphase. Both light and electron microscopy show that the yeast chromosome region is narrower than the flanking mouse DNA. Regions of the yeast insert stain less intensely with propidium iodide than surrounding DNA and bear a morphological resemblance to fragile sites. We investigate the composition of the yeast transgenomes and the modification and chromatin structure of this yeast DNA in mouse cells. We suggest that the underlying basis for the structure we see lies above the level of DNA modification and nucleosome assembly, and may reflect the attachment of the yeast DNA to the rodent cell nucleoskeleton. The yeast integrant replicates late in S phase at a time when G bands of the mouse chromosomes are being replicated, and participates in sister chromatid exchanges at a high frequency. We discuss the implications of these studies to the understanding of how chromatin folding relates to metaphase chromosome morphology and how large stretches of foreign DNA behave when introduced into mammalian cells.
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Hollingsworth, N. M., and B. Byers. "HOP1: a yeast meiotic pairing gene." Genetics 121, no. 3 (March 1, 1989): 445–62. http://dx.doi.org/10.1093/genetics/121.3.445.

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Abstract The recessive mutation, hop1-1, was isolated by use of a screen designed to detect mutations defective in homologous chromosomal pairing during meiosis in Saccharomyces cerevisiae. Mutants in HOP1 displayed decreased levels of meiotic crossing over and intragenic recombination between markers on homologous chromosomes. In contrast, assays of the hop1-1 mutation in a spo13-1 haploid disomic for chromosome III demonstrated that intrachromosomal recombination between directly duplicated sequences was unaffected. The spores produced by SPO13 diploids homozygous for hop1 were largely inviable, as expected for a defect in interhomolog recombination that results in high levels of nondisjunction. HOP1 was cloned by complementation of the spore lethality phenotype and the cloned gene was used to map HOP1 to the LYS11-HIS6 interval on the left arm of chromosome IX. Electron microscopy revealed that diploids homozygous for hop1 fail to form synaptonemal complex, which normally provides the structural basis for homolog pairing. We propose that HOP1 acts in meiosis primarily to promote chromosomal pairing, perhaps by encoding a component of the synaptonemal complex.
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Dissertations / Theses on the topic "Yeast chromosome"

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Morroll, Shaun Michael. "Mapping of yeast artificial chromosomes from Arabidopsis chromosome 5." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308922.

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Bhuiyan, Hasanuzzaman. "Chromosome synapsis and recombination in yeast meiosis /." Stockholm : Institutionen för molekylärbiologi och funktionsgenomik, Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-225.

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Almeida, Hugo Ricardo Noronha de. "Measuring chromosome-end fusions in fission yeast." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2013. http://hdl.handle.net/10362/10629.

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Dissertation presented to obtain the Ph.D degree in Molecular Biology
The ends of eukaryotic chromosomes are protected from illegitimate repair by structures called telomeres. These are comprised of specific DNA repeats bound by a specialized protein complex. When telomere function is compromised, chromosome ends fuse, generating chromosomal abnormalities and genomic instability.(...)
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Yang, Hui. "Chromosome dynamics and chromosomal proteins in relation to apoptotic cell death in yeast." Laramie, Wyo. : University of Wyoming, 2008. http://proquest.umi.com/pqdweb?did=1594496261&sid=1&Fmt=2&clientId=18949&RQT=309&VName=PQD.

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Smith, Victoria. "A molecular genetic analysis of yeast chromosome IX." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239206.

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Priya, Vattem Padma. "Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III." Master's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/4324.

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The linker histone HI binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very significant homology to histone HI. In vitro chromatin assembly experiments with recombinant Hho 1 p have shown that it is able to complex with the dinucleosomes in a similar manner to histone HI. It has also been reported that disruption of HHOl has little affect on RNA levels. A longstanding issue concerns the location of Hho 1 p in the chromatin and studies have shown using immunoprecipitation technique with anti-HA antibody, that Hho 1 p shows a preferential binding to rDNA sequences. In this project we have tried to confirm the above results in wild type cells, using immunopurifi ed anti rHho 1 p antibody.
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Brand, A. H. "Characterisation of a yeast silencer sequence." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377249.

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Lu, Wenqing. "Phenotypic impact of inversions in yeast genome." Electronic Thesis or Diss., Sorbonne université, 2021. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2021SORUS514.pdf.

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Les génomes sont des structures hautement dynamiques et les grandes variations structurelles (SVs) des chromosomes, telles que les inversions, contribuent à l'évolution des génomes et à l'adaptation des espèces. Il est essentiel de comprendre l'impact fonctionnel des inversions sur la diversité phénotypique, car il existe de plus en plus de preuves que les inversions joueraient un rôle important dans les variations phénotypiques. Afin d'expliquer l'impact phénotypique des inversions, nous avons choisi la levure de boulangerie comme modèle eucaryote unicellulaire dans notre travail. Sur la base d'un catalogue de 104 événements d'inversion caractérisés parmi un panel de 142 assemblages de génomes complets, nous nous sommes concentrés sur une inversion spéciale de 32kb localisée sur le chromosome XIV et qui est trouvée de manière récurrente dans diverses souches de Saccharomyces cerevisiae et S. paradoxus. Nous avons utilisé la méthodologie CRISPR/Cas9 d'édition du génome pour générer des bibliothèques de souches de S. cerevisiae contenant cette région dans les deux orientations par l'introduction de 2 cassures double-brin ((DSB pour Double-Strand Break) de l'ADN aux extrémités de cette région. Nous avons construit ce type d'inversions dans 3 souches hôtes présentant des fonds génétiques différents, S288C, YPS128 et Y12. Afin de tester les relations entre ce type de variation génétique et les traits phénotypiques, nous avons étudié l'impact fonctionnel des inversions pendant les cycles cellulaires sexuels et végétatifs, y compris le taux de croissance dans différentes conditions de culture, l'efficacité de la sporulation, l'efficacité des croisements et la viabilité des spores. Ce travail nous a permis de déterminer la contribution de cette inversion aux variations phénotypiques et son rôle adaptatif au cours de l'évolution
Genomes are highly dynamic structures and large-scale Structural Variations (SVs) of chromosomes such as inversions contribute to genome evolution and species adaptation. Understanding the functional impact of inversion on phenotypic diversity is essential because there are growing evidence that inversions play an important role in phenotypic variation. For the purpose of explaining the phenotypic impact of inversions, we choose yeast as single cell eukaryotic model in our work. Based on a catalogue of 104 inversion events characterized among a panel of 142 complete genome assemblies, we focused on a special 32kb inversion on chromosome XIV that is recurrently found in various strains of Saccharomyces cerevisiae and S. paradoxus. CRISPR/Cas9 methodology of genome editing is applied to generate strain libraries in S. cerevisiae containing this region in both orientations through the introduction of DNA double-strand breaks (DSBs) at the inversion boundaries. We constructed such inversion models in 3 different host strains with different genetic background, S288C, YPS128 and Y12. In order to test the relationships between this type of genetic variation and phenotypic traits, we investigated the functional impact of the inversions during both sexual and asexual cell cycles, including growth ratio in different culture conditions, sporulation efficiency, mating efficiency and spore viability. This work allows us to determine the contribution of inversions to phenotypic variations and their adaptive role during evolution
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Collins, Kimberly A. "Characterization of the budding yeast centromeric histone H3 variant, Cse4 /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/5011.

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Hassock, Sheila Ruth. "Physical and transcriptional mapping in the distal Xq28 region of the human X chromosome." Thesis, King's College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312021.

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Books on the topic "Yeast chromosome"

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James, Louise Anne. Physical mapping on human chromosome 3 using yeast artificial chromosomes. Manchester: University of Manchester, 1994.

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Scherer, Stephen W. Physical mapping of human chromosome 7 with yeast artificial chromosome (YAC) vectors. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1991.

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1956-, Nelson David L., and Brownstein Bernard H, eds. YAC libraries: A user's guide. New York: W.H. Freeman and Co., 1994.

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Tracey, S. M. The effects of yeast artificial chromosomes of the yeast genome. Manchester: UMIST, 1995.

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Heale, S. M. Factors effecting the utility of yeast artificial chromosomes as cloning vectors. Manchester: UMIST, 1993.

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1944-, Adolph Kenneth W., ed. Microbial gene techniques. San Diego: Academic Press, 1995.

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Alasdair, MacKenzie, ed. YAC protocols. 2nd ed. Totowa, N.J: Humana Press, 2006.

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Scherer, Stephen W. Physical mapping of human chromosome 7 with yeast artificial chromosomes. 1995.

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(Editor), David L. Nelson, and Bernard Brownstein (Editor), eds. YAC Libraries: A User's Guide (Uwbc Biotechnical Resource). Oxford University Press, USA, 1993.

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Creeper, Leslie Ann. Functional analysis of a mammalian chromosomal origin in yeast. 1985.

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Book chapters on the topic "Yeast chromosome"

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Fabb, Stewart A., and Jiannis Ragoussis. "Yeast artificial chromosome vectors." In Molecular and Cell Biology of Human Gene Therapeutics, 104–24. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0547-7_6.

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Grunstein, Michael, Min Han, Ung-Jin Kim, Tillman Schuster, and Paul Kayne. "Histone and Nucleosome Function in Yeast." In Molecular Biology of Chromosome Function, 347–65. New York, NY: Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3652-8_16.

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Solomon, F., S. Guenette, D. Kirkpatrick, V. Praitis, B. Weinstein, and J. Archer. "A Genetic Analysis of Microtubule Assembly and Function in Yeast." In Chromosome Segregation and Aneuploidy, 199–209. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84938-1_17.

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Diffley, John F. X. "Global regulators of chromosome function in yeast." In Molecular Biology of Saccharomyces, 25–33. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2504-8_3.

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Martin, Gregory B. "Construction of plant yeast artificial chromosome libraries." In Plant Molecular Biology Manual, 383–99. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0511-8_25.

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Scherthan, Harry. "Yeast Chromosome Dynamics Revealed by Immuno FISH." In Springer Protocols Handbooks, 495–510. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-52959-1_50.

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Duffy, Supipi, and Philip Hieter. "The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast." In Methods in Molecular Biology, 11–19. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7306-4_2.

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Joseph, Fraulin, So Jung Lee, Eric Edward Bryant, and Rodney Rothstein. "Measuring Chromosome Pairing During Homologous Recombination in Yeast." In Homologous Recombination, 253–65. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0644-5_18.

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Iba, Koh, Sue Gibson, Sue Hugly, Mitsuo Nishimura, and Chris Somerville. "Chromosome Walking in the Region of Arabidopsis fadD Locus Using Yeast Artificial Chromosomes." In Research in Photosynthesis, 55–58. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-009-0383-8_11.

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Scherthan, Harry, and Caroline Adelfalk. "Live Cell Imaging of Meiotic Chromosome Dynamics in Yeast." In Methods in Molecular Biology, 537–48. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-129-1_31.

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Conference papers on the topic "Yeast chromosome"

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Hieter, Philip, Melanie Bailey, Nigel O'Neil, Derek van Pel, and Peter Stirling. "Abstract IA7: Chromosome instability and synthetic lethality in yeast and cancer." In Abstracts: AACR Precision Medicine Series: Synthetic Lethal Approaches to Cancer Vulnerabilities - May 17-20, 2013; Bellevue, WA. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1535-7163.pms-ia7.

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Terao, Kyohei, Hiroyuki Kabata, Hodehiro Oana, and Masao Washizu. "Manipulation of yeast chromosomal DNA using optically driven microstructures." In SPIE Optics + Photonics, edited by Kishan Dholakia and Gabriel C. Spalding. SPIE, 2006. http://dx.doi.org/10.1117/12.680190.

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Reports on the topic "Yeast chromosome"

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Antonarakis, S. E. Human chromosome 21: Linkage mapping and cloning in yeast artificial chromosomes. Office of Scientific and Technical Information (OSTI), January 1991. http://dx.doi.org/10.2172/6278130.

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Weier, Heinz-Ulrich G., Karin M. Greulich-Bode, Jenny Wu, and Thomas Duell. Delineating Rearrangements in Single Yeast Artificial Chromosomes by Quantitative DNA Fiber Mapping. Office of Scientific and Technical Information (OSTI), September 2009. http://dx.doi.org/10.2172/982923.

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