Relevant bibliographies by topics / Xylitol Production

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Academic literature on the topic 'Xylitol Production'

Author: Grafiati
Published: 18 May 2024

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Journal articles on the topic "Xylitol Production"

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Hong, Yuanyuan, Mehdi Dashtban, Greg Kepka, Sanfeng Chen, and Wensheng Qin. "Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production inTrichoderma reesei." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/169705.

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T. reeseiis an efficient cellulase producer and biomass degrader. To improve xylitol production inTrichoderma reeseistrains by genetic engineering, two approaches were used in this study. First, the presumptive D-xylulokinase gene inT. reesei(xyiH), which has high homology to known fungi D-xylulokinase genes, was silenced by transformation ofT. reeseiQM9414 strain with an antisense construct to create strain S6-2-2. The expression of thexyiHgene in the transformed strain S6-2-2 decreased at the mRNA level, and D-xylulokinase activity decreased after 48 h of incubation. This led to an increase in xylitol production from undetectable levels in wild-typeT. reeseiQM9414 to 8.6 mM in S6-2-2. TheT. reeseiΔxdh is a xylose dehydrogenase knockout strain with increased xylitol production compared to the wild-typeT. reeseiQM9414 (22.8 mM versus undetectable). The copy number of the xylose reductase gene (xyl1) inT. reeseiΔxdh strain was increased by genetic engineering to create a new strain Δ9-5-1. The Δ9-5-1 strain showed a higherxyl1 expression and a higher yield of xylose reductase, and xylitol production was increased from 22.8 mM to 24.8 mM. Two novel strains S6-2-2 and Δ9-5-1 are capable of producing higher yields of xylitol.T. reeseihas great potential in the industrial production of xylitol.
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T.N. Bhagat, A. G. Pathade, and G. R. Pathade. "Xylitol: Production and its applications – A Review." Ecology, Environment and Conservation 30, Suppl (2024): 228–33. http://dx.doi.org/10.53550/eec.2024.v30i02s.048.

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Xylitol is important chemical due to its various applications. The use of xylitol as a sweetener for diabetic patients has made it a high valuable special chemical. As xylitol is present in low concentrations in fruits and vegetables, therefore its extraction is unaffordable from these sources. Thus, xylitol can be produced chemically by reduction of xylose in presence of suitable reducing agents and microbial route method. Although several species of yeast, fungi and bacteria synthesize xylitol by the species of the genus Candida, Saccharomyces, Pichia, Debaryomyces, Trichosporon, Enterobacter, Corynebacterium, Mycobacterium etc. The chemical synthesis of xylitol from xylose is the dominant production method of xylitol production. The industrial process to produce xylitol involves the chemical hydrolysis of D-xylose followed by the hydrogenation of the resultant hemicellulose hydrolysate by catalysts including palladium and nickel. For the chemical synthesis of xylitol, high temperatures and high pressure are required. These processes are very expensive which makes the production costs of synthesizing xylitol because of the highly energy intensive, high temperature, pressure and metal catalyst used for a sustained period of time. Therefore, in this review article we study the microbial route of xylitol production from agricultural residues.
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Kumar, Kuldeep, Ekta Singh, and Smriti Shrivastava. "Microbial xylitol production." Applied Microbiology and Biotechnology 106, no. 3 (January 28, 2022): 971–79. http://dx.doi.org/10.1007/s00253-022-11793-6.

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Hor, Sreyden, Mallika Boonmee Kongkeitkajorn, and Alissara Reungsang. "Evaluation of Xylose-Utilising Yeasts for Xylitol Production from Second-Generation Ethanol Vinasse and Effect of Agitation Intensity in Flask-Scale Xylitol Production." Sains Malaysiana 52, no. 1 (January 31, 2023): 175–85. http://dx.doi.org/10.17576/jsm-2023-5201-14.

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This study aimed to select a yeast strain that effectively utilises xylose to produce xylitol from the vinasse of ethanol broth obtained from the fermentation of sugarcane bagasse hydrolysate. Eleven strains of xylose-fermenting yeasts were evaluated for their abilities to utilise xylose and produce xylitol. Two strains that showed outstanding performance in the semi-defined xylose medium were selected for further testing with a vinasse medium. Candida guilliermondii TISTR 5068 showed a superior xylitol production of 7.03 ± 0.08 g/L with the xylitol yield of 0.70 g/gxylose when cultured in bagasse-based ethanol vinasse. The strain was further tested for its xylitol production performance when cultured at four different agitation intensities. Excessive agitation resulted in a rapid xylitol production rate but caused xylitol consumption once the xylose was depleted. Moderate agitation resulted in the highest xylitol yield of 0.79 g/gxylose. The results of this study have provided important information for the development of the xylitol production process using waste streams from cellulosic ethanol production.
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Hor, Sreyden, Mallika Boonmee Kongkeitkajorn, and Alissara Reungsang. "Sugarcane Bagasse-Based Ethanol Production and Utilization of Its Vinasse for Xylitol Production as an Approach in Integrated Biorefinery." Fermentation 8, no. 7 (July 19, 2022): 340. http://dx.doi.org/10.3390/fermentation8070340.

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Biorefinery of sugarcane bagasse into ethanol and xylitol was investigated in this study. Ethanol fermentation of sugarcane bagasse hydrolysate was carried out by Saccharomyces cerevisiae. After ethanol distillation, the vinasse containing xylose was used to produce xylitol through fermentation by Candida guilliermondii TISTR 5068. During the ethanol fermentation, it was not necessary to supplement a nitrogen source to the hydrolysate. Approximately 50 g/L of bioethanol was produced after 36 h of fermentation. The vinasse was successfully used to produce xylitol. Supplementing the vinasse with 1 g/L of yeast extract improved xylitol production 1.4-fold. Cultivating the yeast with 10% controlled dissolved oxygen resulted in the best xylitol production and yields of 10.2 ± 1.12 g/L and 0.74 ± 0.04 g/g after 60 h fermentation. Supplementing the vinasse with low fraction of molasses to improve xylitol production did not yield a positive result. The supplementation caused decreases of up to 34% in xylitol production rate, 24% in concentration, and 24% in yield.
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West, Thomas P. "Xylitol Production by Candida Species from Hydrolysates of Agricultural Residues and Grasses." Fermentation 7, no. 4 (October 28, 2021): 243. http://dx.doi.org/10.3390/fermentation7040243.

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Xylitol is an industrially important chemical due to its commercial applications. The use of xylitol as a sweetener as well as its utilization in biomedical applications has made it a high value specialty chemical. Although several species of yeast synthesize xylitol, this review focusses on the species of the genus Candida. The importance of the enzyme xylitol reductase present in Candida species as it relates to their ability to synthesize xylitol was examined. Another focus of this work was to review prior studies examining the ability of the Candida species to synthesize xylitol effectively from hydrolysates of agricultural residues and grasses. An advantage of utilizing such a hydrolysate as a substrate for yeast xylitol production would be decreasing the overall cost of synthesizing xylitol. The intent of this review was to learn if such hydrolysates could substitute for xylose as a substrate for the yeast when producing xylitol. In addition, a comparison of xylitol production by Candida species should indicate which hydrolysate of agricultural residues and grasses would be the best substrate for xylitol production. From studies analyzing previous hydrolysates of agricultural residues and grasses, it was concluded that a hydrolysate of sugarcane bagasse supported the highest level of xylitol by Candida species, although corncob hydrolysates also supported significant yeast xylitol production. It was also concluded that fewer studies examined yeast xylitol production on hydrolysates of grasses and that further research on grasses may provide hydrolysates with a higher xylose content, which could support greater yeast xylitol production.
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Meilany, Diah, Dewinka Anugeraheni, Abdul Aziz, Made Tri Ari Penia Kresnowati, and Tjandra Setiadi. "The Effects of Operational Conditions in Scaling Up of Xylanase Enzyme Production for Xylitol Production." Reaktor 20, no. 1 (March 13, 2020): 32–37. http://dx.doi.org/10.14710/reaktor.20.1.32-37.

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The biological route to produce xylitol from Oil Palm Empty Fruit Bunches (EFBs) comprises of EFBs pretreatment, enzymatic hydrolysis, fermentation, and downstream separation of the produced xylitol. Due to the specificity in the hemicellulose composition of EFBs, a xylanase enzyme that has a high affinity to EFBs is required to hydrolyze the EFBs into xylose. In this research, the influences of aeration, humidity, and mixing in xylanase production were mapped. The xylanase production was performed by Aspergillus fumigatus ITBCCL170 in a solid-state fermentation using a tray fermenter with EFBs as the substrate. The optimal configuration was further scaled up into xylanase production using 1000 g of EFBs as the substrate. The results showed that the highest enzyme activity was 236.3 U/g EFB, obtained from the use of humid air airflow of 0.1 LPM, and mixing was performed once a day. The scaling up resulted in a lower xylanase activity and call for a better design of the fermenter.Keywords: aeration, humidity, mixing, OPEFBs, tray fermenter, xylanase, xylitol
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Rahayu, E., N. Hidayah, and R. S. Adiandri. "Production of Xylitol from Corn Biomass using Candida sp. As Microbial Agent." IOP Conference Series: Earth and Environmental Science 1024, no. 1 (May 1, 2022): 012075. http://dx.doi.org/10.1088/1755-1315/1024/1/012075.

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Abstract Xylitol, C5H12O5, is a white and odorless crystalline powder of sweetening agents that included as low-calorie sweetener. It could be used as a healthy ingredient for food and pharmaceutical. Natural sources of xylitol are fruit and vegetable, even in minute quantities. At industrial scale, xylitol is produced through hydrolysis and hydrogenation process of lignocellulosic materials. The aim of this research was to study the production of xylitol from corn biomass using Candida sp. as a microbial agent. The research was conducted using different species of Candida sp. (C. guilliermondii and C. tropicalis) and supplement media growth (with or without glucose). Xylitol concentration was examined after fermentation for 3 and 5 days. The results showed that corncob is a promising material to use in producing xylitol from lignocellulosic biomass. The longer fermentation time, the higher xylitol concentration, ranged from 0.049 to 0.088 g/L. However, compare to another microbe species, the treatment using Candida tropicalis showed that long fermentation resulted in a lower xylitol concentration. The addition of glucose as co-substrate increased xylose consumption rate and xylitol productivity. These results provide useful information to develop further study about xylitol production using agricultural biomass.
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Mardawati, Efri, Budi Mandra Harahap, Emilda Ayu Febrianti, Agus Try Hartono, Natasha Putri Siahaan, Anting Wulandari, Silvia Yudiastuti, Sri Suhartini, and Kasbawati Kasbawati. "Integrated and partial process of xylitol and bioethanol production from oil palm empty fruit bunches." Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering 5, no. 1 (July 31, 2022): 49–67. http://dx.doi.org/10.21776/ub.afssaae.2022.005.01.5.

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Oil palm empty fruit bunches (OPEFBs) are highly abundant in Indonesia and have been highlighted as a potential feedstock for bioethanol and xylitol production. However, the efficacy of the fermentation technology to convert OPEFBs to bioethanol and xylitol, either in partial (i.e. mono-production) or integrated (i.e. co-production) process, still needs further improvement. This study aimed to evaluate the partial and integrated process for xylitol and bioethanol production from OPEFBs. In the integrated process, the remaining solid residues after xylitol extraction are used as feedstock for bioethanol due to their high cellulose compounds. This solid residue is more susceptible to be degraded by cellulase enzymes into glucose and further transformed into bioethanol. In the partial process of xylitol production, xylanase enzyme was used to hydrolyze xylan into xylose, which was then converted into xylitol using Debaryomyces hansenii. While in the partial process of bioethanol production, the hydrolysis of cellulose in the OPEFB into glucose was carried out using cellulase enzymes, followed by fermentation using Saccharomyces cerevisiae. The results show that the partial process produced xylitol yield (Yp/s) of 0.10 g-xylitol/g-xylose, while bioethanol at yield (Yp/s) of 0.32 g-bioethanol/g-glucose, respectively. The integrated process generates xylitol yield (Yp/s)of 0.298 g-xylitol/g-xylose, with bioethanol yield from the remaining solid at 0.051 g-bioethanol/g-OPEFB (or 0.078 g-bioethanol/g-glucose). These findings, therefore, confirmed that the integrated process of xylitol with bioethanol production might offer higher efficacy of OPEFB utilization into high value-added products.
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Ambarsari, Laksmi, Suryani Suryani, Steffanus Gozales, and Puspa Julistia Puspita. "The Addition Effects of Glucose as a Co-substrate on Xylitol Production by Candida guilliermondii." Current Biochemistry 2, no. 1 (April 20, 2015): 13–21. http://dx.doi.org/10.29244/cb.2.1.13-21.

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High cost production is one of the constraints of the commercial xylitol production due to high energy needed and pure raw materials. Therefore, it is necessary to improve the xylitol production eficiently with lower production cost by using microorganisms. The research objectives were to determine the optimum xylitol production from xylose by metabolism of C. guilliermondii and effect of glucose as a co-substrate in fermentation medium. The ratio of glucose : xylose (g/L) was 1:25, 1:12, 1:5 and 1:2.5 respectively. The xylitol concentration was measured by spectrophotometer method (D-sorbytol/D-xylitol kit). The result showed that the exponential phase of Candida guilliermondii was 12 h to 36 of incubation and optimum of incubation time to produce the highest xylitol was 72 h. The best ratio- of glucose : xylose to produce xylitol was 9 g/L glucose : 45 g/L xylose (1 : 5). The xylitol concentration produced from medium with the addition of glucose was 2.85 g/L. This concentration increased five times compared to that in the medium without addition of glucose that only reached 2.85 g/L. According to this study, the addition of glucose as a co-substrate could increase the xylitol production.
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Dissertations / Theses on the topic "Xylitol Production"

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Rangaswamy, Sendil. "Xylitol Production From D-Xylose by Facultative Anaerobic Bacteria." Diss., Virginia Tech, 2003. http://hdl.handle.net/10919/26385.

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Seventeen species of facultative anaerobic bacteria belonging to three genera (Serratia, Cellulomonas, and Corynebacterium) were screened for the production of xylitol; a sugar alcohol used as a sweetener in the pharmaceutical and food industries. A chromogenic assay of both solid and liquid cultures showed that 10 of the 17 species screened could grow on D-xylose and produce detectable quantities of xylitol during 24-96 h of fermentation. The ten bacterial species were studied for the effect of environmental factors, such as temperature, concentration of D-xylose, and aeration, on xylitol production. Under most conditions, Corynebacterium sp. NRRL B 4247 produced the highest amount of xylitol. The xylitol produced by Corynebacterium sp. NRRL B 4247 was confirmed by mass spectrometry. Corynebacterium sp. NRRL B 4247 was studied for the effect of initial D-xylose concentration, glucose, glyceraldehyde, and gluconate, aeration, and growth medium. Corynebacterium sp. NRRL B 4247 produced xylitol only in the presence of xylose, and did not produce xylitol when gluconate or glucose was the substrate. The highest yield of xylitol produced in 24 h (0.57 g/g xylose) was using an initial D-xylose concentration of 75 g/l. Under aerobic conditions the highest xylitol yield was 0.55 g/g while under anaerobic conditions the highest yield was 0.2 g/g. Glyceraldehyde in concentrations greater than 1 g/l inhibited Corynebacterium sp. B 4247 growth and xylitol production. Corynebacterium sp. NRRL B 4247 culture grown in the presence of potassium gluconate (96 g/l) for 48 h and on addition of D-xylose to the media increased accumulation to 10.1 g/l of xylitol after 150 h. Corynebacterium sp. NRRL B 4247 exhibited both NADH and NADPH-dependent xylose reductase activity in cell-free extracts. The NADPH-dependent activity was substrate dependent. The activity was 2.2-fold higher when DL-glyceraldehyde was used as substrate than with D-xylose. In cell-free extracts the difference in xylose reductase and xylitol dehydrogenase activity was highest at 24 h, whereas for cell cultures that were grown in gluconate and xylose, the difference in the reductase and dehydrogenase activities was highest at 12 h after xylose addition. The NAD+ dependent xylitol dehydrogenase activity was low compared to the cells grown without gluconate. The molecular weight of NADPH-dependent xylose reductase protein obtained by gel filtration chromatography was 58 kDa. Initial purification was performed on a DE-52 anion exchange column. Purification using Red Sepharose affinity column resulted in a 58 kDa protein on the SDS PAGE gel and was further purified on a Mono-Q column. The activity stained band on the native gel yielded 58, 49, 39 and 30 kDa bands on the denaturing gel. The peptides of the 58 kDa protein of Corynebacterium sp. B 4247 sequenced by mass spectrometry, identified with E2 and E3 (Bacillus subtilis) components of multi-enzyme system consisting of pyruvate dehydrogenase complex, 2-oxoglutarate dehydrogenase complex and oxo-acid dehydrogenase complex. A 75% match was shown by the peptide â QMSSLVTRâ with E-value of 8e-04 to the Saccharomyces cerevisiae protein that was capable of reducing xylose to xylitol. The peptide â LLNDPQLILMEAâ had conserved match â LL + DPâ over several aldose reductases. The xylose reductase of the yeast Candida tropicalis ATCC 96745 was also purified. The molecular weight of the yeast NADPH-dependent xylose reductase was about 37 kDa on an SDS PAGE
Ph. D.
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Kuusisto, Jyrki. "Catalytic production of alternative sweeteners : lactitol, mannitol and xylitol /." Åbo : Åbo Akademi University, 2006. http://catalogue.bnf.fr/ark:/12148/cb414423531.

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Saha, Shyama Prasad. "Production of microbial xylanase under submerged fermentation of agro-residues and its application in xylitol production." Thesis, University of North Bengal, 2018. http://ir.nbu.ac.in/hdl.handle.net/123456789/2682.

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Povelainen, Mira. "Pentitol phosphate dehydrogenases discovery, characterization and use in D-arabitol and xylitol production by metabolically engineered Bacillus subtilis /." Helsinki : University of Helsinki, 2008. http://urn.fi/URN:ISBN:978-952-10-5095-4.

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Nolleau, Valérie. "Caractérisation du métabolisme du xylose en vue d'une optimisation de la production de xylitol chez "Candida guilliermondii" et "Candida parapsilosis"." Montpellier 2, 1994. http://www.theses.fr/1994MON20284.

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En vue de produire du xylitol a partir d'un hydrolysat hemicellulosique riche en xylose, nous avons tout d'abord caracterise et quantifie les reponses cinetiques de deux especes levuriennes candida guillermondii et candida parapsilosis, souches qui ont ete choisies pour leurs aptitudes a fermenter le xylose en xylitol. Cette etude, realisee en milieu synthetique et dans differentes conditions de culture, a abouti a la definition des conditions optimales pour la production de xylitol. Ainsi, avec candida guilliermondii, le rendement de production en xylitol atteint une valeur maximale de 0,67 g/g en presence de 300 g/l de xylose, a un ph de 6,0 et avec une vitesse de transfert d'oxygene de 2,2 mmol/l. H. Les conditions optimales de production de xylitol par candida parapsilosis (y#p#/#s = 0,75 g/g) sont obtenues lors de cultures realisees avec 100 g/l de xylose, a un ph initial de 4,75 et pour une vitesse de transfert d'oxygene de 0,4 mmol/l. H. En etudiant le comportement des deux souches dans un milieu reproduisant un hydrolysat hemicellulosique c'est-a-dire contenant principalement du glucose et du mannose, nous avons pu analyser l'influence de ces differents substrats sur la production de xylitol. Nous avons pu observer que la consommation du glucose en conditions aerobies par ces levures conduisait en fait a l'obtention d'une biomasse cellulaire nettement mieux adaptee pour produire du xylitol a partir de xylose. Nous nous sommes donc bases sur cette particularite pour ameliorer la fermentescibilite d'un hydrolysat hemicellulosique de peuplier. Outre cette approche physiologique, les reactions enzymatiques directement impliquees dans l'accumulation du xylitol ont ete etudiees. La comparaison de l'expression de la xylose reductase et de la xylitol deshydrogenase mesuree in vitro avec la production de xylitol mesuree in vivo a permis de souligner l'importance de l'equilibre de la balance d'oxydo-reduction dans une cellule
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Agrawal, Manoj. "Metabolic engineering of Zymomonas mobilis for improved production of ethanol from lignocelluloses." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/43618.

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Ethanol from lignocellulosic biomass is a promising alternative to rapidly depleting oil reserves. However, natural recalcitrance of lignocelluloses to biological and chemical treatments presents major engineering challenges in designing an ethanol conversion process. Current methods for pretreatment and hydrolysis of lignocelluloses generate a mixture of pentose (C5) and hexose (C6) sugars, and several microbial growth inhibitors such as acetic acid and phenolic compounds. Hence, an efficient ethanol production process requires a fermenting microorganism not only capable of converting mixed sugars to ethanol with high yield and productivity, but also having high tolerance to inhibitors. Although recombinant bacteria and yeast strains have been developed, ethanol yield and productivity from C5 sugars in the presence of inhibitors remain low and need to be further improved for a commercial ethanol production. The overarching objective of this work is to transform Zymomonas mobilis into an efficient whole-cell biocatalyst for ethanol production from lignocelluloses. Z. mobilis, a natural ethanologen, is ideal for this application but xylose (a C5 sugar) is not its 'natural' substrate. Back in 1995, researches at National Renewable Energy Laboratory (NREL) had managed to overcome this obstacle by metabolically engineering Z. mobilis to utilize xylose. However, even after more than a decade of research, xylose fermentation by Z. mobilis is still inefficient compared to that of glucose. For example, volumetric productivity of ethanol from xylose fermentation is 3- to 4- fold lower than that from glucose fermentation. Further reduction or complete inhibition of xylose fermentation occurs under adverse conditions. Also, high concentrations of xylose do not get metabolized completely. Thus, improvement in xylose fermentation by Z. mobilis is required. In this work, xylose fermentation in a metabolically engineered Z. mobilis was markedly improved by applying the technique of adaptive mutation. The adapted strain was able to grow on 10% (w/v) xylose and rapidly ferment xylose to ethanol within 2 days and retained high ethanol yield. Similarly, in mixed glucose-xylose fermentation, the strain produced a total of 9% (w/v) ethanol from two doses of 5% glucose and 5% xylose (or a total of 10% glucose and 10% xylose). Investigation was done to identify the molecular basis for efficient biocatalysis. An altered xylitol metabolism with reduced xylitol formation, increased xylitol tolerance and higher xylose isomerase activity were found to contribute towards improvement in xylose fermentation. Lower xylitol production in adapted strain was due to a single mutation in ZMO0976 gene, which drastically lowered the reductase activity of ZMO0976 protein. ZMO0976 was characterized as a novel aldo-keto reductase capable of reducing xylose, xylulose, benzaldehyde, furfural, 5-hydroxymethyl furfural, and acetaldehyde, but not glucose or fructose. It exhibited nearly 150-times higher affinity with benzaldehyde than xylose. Knockout of ZMO0976 was found to facilitate the establishment of xylose fermentation in Z. mobilis ZM4. Equipped with molecular level understanding of the biocatalytic process and insight into Z. mobilis central carbon metabolism, further genetic engineering of Z. mobilis was undertaken to improve the fermentation of sugars and lignocellulosic hydrolysates. These efforts culminated in construction of a strain capable of fermenting glucose-xylose mixture in presence of high concentration of acetic acid and another strain with a partially operational EMP pathway.
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Rissi, Silvana. "Avaliação do potencial de produção de etanol e xilitol a partir de xilose por macromicetos." reponame:Repositório Institucional da UCS, 2016. https://repositorio.ucs.br/handle/11338/2360.

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Chin, Zhao Si, and 趙士慶. "Production of Xylitol from Xylose Fermentation." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/35004117867933016450.

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碩士
大葉大學
食品工程研究所
87
Xylitol has multiple biological functions that render the sugar alcohol many potential applic-ations in the food industry. This research used the yeast, Candida subtropicalis C22 , isolated from sugar can bagasse to ferment xylose into xylitol. The strain produced mostly xylitol with very small amount of ethanol. Shaker flasks of working volume of 150ml were used for the study. The strain could produce 17.5% (w/v) xylitol with initial xylose concentration of 20% (w/v) within 9 days. The addition of surfactant (Triton X-100) was found to siguificantly speed up the fermentation,similar xylitol conc.(16% w/w) was achieved in 5 days. However, the yield was slightly decreased. The productivity was 0.0359g/hr/L/g dry cell. Key Words：Xylose、Xylitol、surfactant、Candida subtropicalis
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Chen, Kaun-Ben, and 陳觀彬. "Production of xylitol by immobilized yeast cells." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/54084061463037157527.

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碩士
國立雲林科技大學
工業化學與災害防治研究所碩士班
89
Production of xylitol by immobilized yeast cell Student：Kaun-Ben Chen Advisors：Dr. Wen-Chang Liaw Institute of Industrial Chemistry and Hazards Prevention National Yunlin University of Science & Technology ABSTRACT This study deals with production of xylitol from rice straw. Straw was first treated with sulfuric acid, the result indicates that the best condition for hydrolysis is using 2% H2SO4 and heat the straw at 126℃for 60min.Througth this treatment 13.3g of xylose can be obtained from 100g of rice straw. Xylose thus obtained is further decolorized with activated charcol and pH adjusted to remove the salts. Meanwhile, the yeast strain-Candida subtropicalis is immobilized and entrapped in the hydrophilic acrylic resin matrix using photopolymerization method. The immobilized cells are then used to ferment xylose to xylitol. As for immobilization with photo-crosslinking, the raw material used are acrylic monomer such as polyethylene glycol diacrylate ( PEG-DA) and 2-hydroxyethyl methacrylate (HEMA).To these substances were added with 1% Benzoin isopropyl ether(photo-semsitizer agent)and yeast cells. The membrarce thus formed has a thickness of 0.2mm and in the 10% culture medium it will product the highest amount of xylitol with a yield of 70%. The immobilized yeast cells are them treated batchwise for endurance. Result indicates that it is fairly stable for 1~2 months with a yield exceeds 60%.
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Serrano, Patrícia Isabel Pós-de-Mina. "Production of xylitol by the yeast Komagataella pastoris." Master's thesis, 2018. http://hdl.handle.net/10362/55075.

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Xylitol is a sugar alcohol that can replace sucrose, is tolerated by diabetics and has several clinical applications. Currently, xylitol is manufactured on a large scale through a chemical process, but there is a search for alternative processes that are environmentally friendly and more cost-effective. In this thesis, the biotechnological production of xylitol has been examined as an alternative to the chemical process, using the yeast Komagataella pastoris to produce xylitol from glucose/xylose mixtures. Different parameters, namely, pH, temperature, xylose concentration, the presence of arabinose as substrate and dissolved oxygen were tested. This work demonstrated that the best pH value for xylitol synthesis was 7.0 - 7.5 that resulted in 4.04 g/L of xylitol, with a volumetric productivity of 0.024 g/L.h and a specific productivity 0.35 gxylitol /gCDW. Cultivation with initial pH value 7.00 and 37 ºC, in fully aerobic conditions, and changing the pH to 6.4 at 72 h of cultivation, resulted in the highest xylitol concentration of this study: 12.00 g/L, concomitant with a volumetric productivity of 0.071 g/L.h and a specific productivity of 1.41 gxylitol/gCDW. Limiting oxygen conditions resulted in a xylitol concentration of 5.81 g/L, with a specific productivity of 0.33 gxylitol/ gCDW, and a volumetric productivity, of 0.035 g/L.h. Several concentrations of xylose in the glucose/xylose mixtures were tested. The highest xylitol production was obtained with 60 g/L of xylose. Reducing the xylose concentration resulted in lower xylitol production. Interestingly, it was observed for the first time that K. pastoris was able to synthesize arabitol using arabinose as substrate, with the arabitol concentration reaching 3.15 g/L on cultivation at 37 ºC under oxygen limitation. This feature opens up the possibility of using this process for the synthesis of both sugar alcohols, xylitol and arabitol, being apparently possible to tune the synthesis of each product by altering the cultivation conditions.
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Books on the topic "Xylitol Production"

1

Ojamo, Heikki. Yeast xylose metabolism and xylitol production. Espoo, Finland: Technical Research Centre of Finland, 1994.

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de Almeida Felipe, Maria das Graças, and Anuj Kumar Chandel, eds. Current Advances in Biotechnological Production of Xylitol. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-04942-2.

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Toivari, Mervi. Engineering the pentose phosphate pathway of Saccharomyces cerevisiae for production of ethanol and xylitol. [Espoo, Finland]: VTT Technical Research Centre of Finland, 2007.

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Maria das Graças de Almeida Felipe and Anuj Kumar Chandel. Current Advances in Biotechnological Production of Xylitol: Fermentative Production of Xylitol. Springer International Publishing AG, 2022.

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Chandel, Anuj Kumar, and Silvio Silvério da Silva. D-Xylitol: Fermentative Production, Application and Commercialization. Springer London, Limited, 2012.

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Chandel, Anuj Kumar, and Silvio Silvério da Silva. D-Xylitol: Fermentative Production, Application and Commercialization. Springer, 2014.

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Book chapters on the topic "Xylitol Production"

1

Salgado, José Manuel, Attilio Converti, and José Manuel Domínguez. "Fermentation Strategies Explored for Xylitol Production." In D-Xylitol, 161–91. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_7.

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Sasaki, Miho, Masayuki Inui, and Hideaki Yukawa. "Microorganisms for Xylitol Production: Focus on Strain Improvement." In D-Xylitol, 109–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_5.

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Converti, Attilio, Patrizia Perego, José Manuel Domínguez González, Janaína Teles de Faria, and Fábio Coelho Sampaio. "Bioenergetic Aspects of Xylitol Production from Lignocellulosic Materials." In D-Xylitol, 205–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_9.

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Hou-Rui, Zhang. "Key Drivers Influencing the Large Scale Production of Xylitol." In D-Xylitol, 267–89. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_12.

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de Freitas Branco, Ricardo, Anuj K. Chandel, and Sílvio Silvério da Silva. "Enzymatic Production of Xylitol: Current Status and Future Perspectives." In D-Xylitol, 193–204. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_8.

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Mpabanga, Tandiwe P., Anuj K. Chandel, Silvio Silvério da Silva, and Om V. Singh. "Detoxification Strategies Applied to Lignocellulosic Hydrolysates for Improved Xylitol Production." In D-Xylitol, 63–82. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_3.

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Ravella, Sreenivas Rao, Joe Gallagher, Steve Fish, and Reddy Shetty Prakasham. "Overview on Commercial Production of Xylitol, Economic Analysis and Market Trends." In D-Xylitol, 291–306. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_13.

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de Cássia Lacerda Brambilla Rodrigu, Rita, Eliana Vieira Canettieri, Ernesto Acosta Martinez, Larissa Canilha, Ana Irene Napolez Solenzal, and João Batista de Almeida e Silva. "Statistical Approaches for the Optimization of Parameters for Biotechnological Production of Xylitol." In D-Xylitol, 133–60. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31887-0_6.

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Saha, Badal C., and Rodney J. Bothast. "Microbial Production of Xylitol." In ACS Symposium Series, 307–19. Washington, DC: American Chemical Society, 1997. http://dx.doi.org/10.1021/bk-1997-0666.ch017.

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Lugani, Yogita, Balwinder Singh Sooch, Vinita Dheeran, and Sachin Kumar. "Microbial Production of Xylitol." In Microbial Fermentation and Enzyme Technology, 227–56. Boca Raton : CRC Press, [2020]: CRC Press, 2020. http://dx.doi.org/10.1201/9780429061257-15.

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Conference papers on the topic "Xylitol Production"

1

Qi, Xianghui, Jing Lin, Xu Wang, Fei Wang, Wenying Deng, Bokai Zhao, Xiu Wang, and Jianzong Meng. "Introduction of two key enzymes: D-arabitol dehydrogenase and xylitol dehydrogenase for microbial production of xylitol." In International conference on Human Health and Medical Engineering. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/hhme130431.

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Majid Soleimani, Lope Tabil, and Satya Panigrahi. "Xylitol Production in Aerated Free- and Immobilized-cell Systems." In 2013 Kansas City, Missouri, July 21 - July 24, 2013. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2013. http://dx.doi.org/10.13031/aim.20131620511.

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ZHANG, Huanhuan, Junhua YUN, Tinashe Archbold MAGOCHA, Miaomiao YANG, Yanbo XUE, and Xianghui QI. "Microbial Production of Xylitol from D-arabitol by Gluconobacter Oxydans." In International Conference on Biological Engineering and Pharmacy 2016 (BEP 2016). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/bep-16.2017.23.

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Sasaki, Chizuru, Akihiro Kurosumi, Yuya Yamashita, Godliving Mtui, and Yoshitoshi Nakamura. "Xylitol production from dilute-acid hydrolysis of bean group shells." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0132.

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M Soleimani, L Tabil, and S Panigrahi. "Bio-production of a Polyalcohol (Xylitol) from Lignocellulosic Resources: A Review." In 2006 CSBE/SCGAB, Edmonton, AB Canada, July 16-19, 2006. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2006. http://dx.doi.org/10.13031/2013.22064.

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Setty, Yelamarthi Pydi, and Katuri Srivani. "Effect of Inoculum Age and Volume on Microbial Production of Xylitol." In 14th Asia Pacific Confederation of Chemical Engineering Congress. Singapore: Research Publishing Services, 2012. http://dx.doi.org/10.3850/978-981-07-1445-1_230.

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Michael Mueller, Mark R Wilkins, and Ibrahim M Banat. "Screening Of Kluyveromyces marxianus IMB Strains At Microaerophilic Conditions For Xylitol Production." In 2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2010. http://dx.doi.org/10.13031/2013.29667.

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"Modeling Fermentative Production of Xylitol in an Undefined Medium Derived from Biomass." In 2014 ASABE Annual International Meeting. American Society of Agricultural and Biological Engineers, 2014. http://dx.doi.org/10.13031/aim.20141913384.

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Pramasari, Dwi Ajias, Maulida Oktaviani, M. Zuvan Maulana Fahrezi, Ahmad Thontowi, Atit Kanti, and Euis Hermiati. "Short-term Meyerozyma caribbica Y67 adaptation in sugarcane trash hemicellulosic hydrolysate for xylitol production." In THE 2ND INTERNATIONAL CONFERENCE OF LIGNOCELLULOSE. AIP Publishing, 2024. http://dx.doi.org/10.1063/5.0184684.

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Michael Mueller, Mark R Wilkins, and Ibrahim M Banat. "Production of Ethanol and Xylitol by Thermotolerant Kluyveromyces marxianus Strains using Xylose at 40 and 45°C." In 2008 Providence, Rhode Island, June 29 - July 2, 2008. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2008. http://dx.doi.org/10.13031/2013.24749.

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