Relevant bibliographies by topics / Widefield motion

Academic literature on the topic 'Widefield motion'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Widefield motion"

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Amato, Alessia, Francesco Nadin, Federico Borghesan, Maria Vittoria Cicinelli, Irini Chatziralli, Saena Sadiq, Rukhsana Mirza, and Francesco Bandello. "Widefield Optical Coherence Tomography Angiography in Diabetic Retinopathy." Journal of Diabetes Research 2020 (November 24, 2020): 1–10. http://dx.doi.org/10.1155/2020/8855709.

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Purpose. To summarize the role of widefield optical coherence tomography angiography (WF-OCTA) in diabetic retinopathy (DR), extending from the acquisition strategies to the main clinical findings. Methods. A PubMed-based search was carried out using the terms “Diabetic retinopathy”, “optical coherence tomography angiography”, “widefield imaging”, and “ultra-widefield imaging”. All studies published in English up to August 2020 were reviewed. Results. WF-OCTA can be obtained with different approaches, offering advantages over traditional imaging in the study of nonperfusion areas (NPAs) and neovascularization (NV). Quantitative estimates and topographic distribution of NPA and NV are useful for treatment monitoring and artificial intelligence-based approaches. Curvature, segmentation, and motion artifacts should be assessed when using WF-OCTA. Conclusions. WF-OCTA harbors interesting potential in DR because of its noninvasiveness and capability of objective metrics of retinal vasculature. Further studies will facilitate the migration from traditional imaging to WF-OCTA in both the research and clinical practice fields.
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Liebel, Matz, Franco V. A. Camargo, Giulio Cerullo, and Niek F. van Hulst. "Widefield phototransient imaging for visualizing 3D motion of resonant particles in scattering environments." Nanoscale 14, no. 8 (2022): 3062–68. http://dx.doi.org/10.1039/d1nr06837g.

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We show how ultrafast holographic transient microscopy can be used to identify, visualise and 3D track dynamically moving non-fluorescent nanoparticles in large volumes-of-view and in the presence of non-specific scattering background.
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Li, Ziwei, Qinrong Zhang, Shih-Wei Chou, Zachary Newman, Raphaël Turcotte, Ryan Natan, Qionghai Dai, Ehud Y. Isacoff, and Na Ji. "Fast widefield imaging of neuronal structure and function with optical sectioning in vivo." Science Advances 6, no. 19 (May 2020): eaaz3870. http://dx.doi.org/10.1126/sciadv.aaz3870.

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Optical microscopy, owing to its noninvasiveness and subcellular resolution, enables in vivo visualization of neuronal structure and function in the physiological context. Optical-sectioning structured illumination microscopy (OS-SIM) is a widefield fluorescence imaging technique that uses structured illumination patterns to encode in-focus structures and optically sections 3D samples. However, its application to in vivo imaging has been limited. In this study, we optimized OS-SIM for in vivo neural imaging. We modified OS-SIM reconstruction algorithms to improve signal-to-noise ratio and correct motion-induced artifacts in live samples. Incorporating an adaptive optics (AO) module to OS-SIM, we found that correcting sample-induced optical aberrations was essential for achieving accurate structural and functional characterizations in vivo. With AO OS-SIM, we demonstrated fast, high-resolution in vivo imaging with optical sectioning for structural imaging of mouse cortical neurons and zebrafish larval motor neurons, and functional imaging of quantal synaptic transmission at Drosophila larval neuromuscular junctions.
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Ma, Yayao, Youngjae Lee, Catherine Best-Popescu, and Liang Gao. "High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells." Proceedings of the National Academy of Sciences 118, no. 3 (January 11, 2021): e2004176118. http://dx.doi.org/10.1073/pnas.2004176118.

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We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale.
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Matsui, Yoshitsugu, Atsushi Ichio, Asako Sugawara, Eriko Uchiyama, Hitomi Suimon, Hisashi Matsubara, Masahiko Sugimoto, Kengo Ikesugi, and Mineo Kondo. "Comparisons of Effective Fields of Two Ultra-Widefield Ophthalmoscopes, Optos 200Tx and Clarus 500." BioMed Research International 2019 (December 5, 2019): 1–7. http://dx.doi.org/10.1155/2019/7436293.

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Purpose. To compare the effective fields of the Optos 200Tx® and Clarus 500™, two ultra-widefield ophthalmoscopes, based on their ability to image branches of retinal vessel in the four retinal quadrants. Methods. Ninety retinal images from 90 patients with various eye diseases were studied. All patients had undergone 200° retinal imaging to obtain a single image of Optos (O) and the montage of two images of the Clarus (C). The highest number of traceable vessel branches in the four retinal quadrants was determined by two masked raters. An image was classified as “O > C” when the number of identifiable branch was greater in the Optos than the Clarus, as “O = C” when the number was equal and as “O < C” when the number was fewer in the Optos than the Clarus. Results. The appearance probability of “O > C” was significantly higher at the upper temporal quadrant than “O < C” (p<0.01 for both raters). In contrast, the appearance probability of “O < C” was significantly higher at the lower nasal quadrant than “O > C” (p<0.01 for both raters). There were no significant differences in the appearance probability between “O > C” and “O < C” at the other two retinal quadrants (p>0.50 for both raters). Conclusions. These results demonstrate that the effective field of views was different between the two devices at different retina quadrants. Further studies are needed to clarify possible factors such as artifacts by the eyelashes, differences in the depth of focus, motion of the device, and different locations of the images on the effective field of views.
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Morgan, Darrell D., and Anthony G. Moss. "The Effects of Cigarette Smoke on Porcine Airway Epithelium." Microscopy and Microanalysis 4, S2 (July 1998): 1076–77. http://dx.doi.org/10.1017/s1431927600025502.

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Cephalad pulmonary mucociliary clearance driven by cilia of the ciliated airway epithelium provides probably the most important line of defense against inhaled toxins and particulate material for organs of the thoracic cavity. We demonstrate the reorganization of the cytoskeleton and the endoplasmic reticulum of airway epithelial cells upon cigarette smoke inhalation by employing DIC, LSCM, TEM and widefield fluorescence microscopy and have correlated this reorganization to changes in ciliary beat frequency (CBF) via FFT analysis. Neonatal pigs were used to provided healthy tracheal epithelial tissue. Exposure to cigarette smoke causes rapid ultrastructural changes including: ciliary distortion and detachment, ciliary abscission and severe alteration in endoplasmic reticulum structure suggesting profound disruption of essential membrane-cytoskeletal linkages.To examine changes in CBF we describe a simple approach, using a laser scanning confocal microscope (LSCM) and an analog-to-digital computer converter/analyzer for the acquisition of data from biological systems that undergo rapid periodic movement, such as ciliary motion.
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Robertson, Islay O., Cheng Tan, Sam C. Scholten, Alexander J. Healey, Gabriel J. Abrahams, Guolin Zheng, Aurelien Manchon, Lan Wang, and Jean-Philippe Tetienne. "Imaging current control of magnetization in Fe3GeTe2 with a widefield nitrogen-vacancy microscope." 2D Materials, December 14, 2022. http://dx.doi.org/10.1088/2053-1583/acab73.

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Abstract Van der Waals (vdW) magnets are appealing candidates for realising spintronic devices that exploit current control of magnetization (e.g. switching or domain wall motion), but so far experimental demonstrations have been sparse, in part because of challenges associated with imaging the magnetization in these systems. Widefield nitrogen-vacancy (NV) microscopy allows rapid, quantitative magnetic imaging across entire vdW flakes, ideal for capturing changes in the micromagnetic structure due to an electric current. Here we use a widefield NV microscope to study the effect of current injection in thin flakes (∽10 nm) of the vdW ferromagnet Fe3GeTe2 (FGT). We first observe current-reduced coercivity on an individual domain level, where current injection in FGT causes substantial reduction in the magnetic field required to locally reverse the magnetisation. We then explore the possibility of current-induced domain-wall motion, and provide preliminary evidence for such a motion under relatively low current densities, suggesting the existence of strong current-induced torques in our devices. Our results illustrate the applicability of widefield NV microscopy to imaging spintronic phenomena in vdW magnets, highlight the possibility of efficient magnetization control by direct current injection without assistance from an adjacent conductor, and motivate further investigations of the effect of currents in FGT and other vdW magnets.
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Leibbrandt, Richard, Sarah Nicholas, and Karin Nordström. "The impulse response of optic flow-sensitive descending neurons to roll m-sequences." Journal of Experimental Biology 224, no. 23 (December 1, 2021). http://dx.doi.org/10.1242/jeb.242833.

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ABSTRACT When animals move through the world, their own movements generate widefield optic flow across their eyes. In insects, such widefield motion is encoded by optic lobe neurons. These lobula plate tangential cells (LPTCs) synapse with optic flow-sensitive descending neurons, which in turn project to areas that control neck, wing and leg movements. As the descending neurons play a role in sensorimotor transformation, it is important to understand their spatio-temporal response properties. Recent work shows that a relatively fast and efficient way to quantify such response properties is to use m-sequences or other white noise techniques. Therefore, here we used m-sequences to quantify the impulse responses of optic flow-sensitive descending neurons in male Eristalis tenax hoverflies. We focused on roll impulse responses as hoverflies perform exquisite head roll stabilizing reflexes, and the descending neurons respond particularly well to roll. We found that the roll impulse responses were fast, peaking after 16.5–18.0 ms. This is similar to the impulse response time to peak (18.3 ms) to widefield horizontal motion recorded in hoverfly LPTCs. We found that the roll impulse response amplitude scaled with the size of the stimulus impulse, and that its shape could be affected by the addition of constant velocity roll or lift. For example, the roll impulse response became faster and stronger with the addition of excitatory stimuli, and vice versa. We also found that the roll impulse response had a long return to baseline, which was significantly and substantially reduced by the addition of either roll or lift.
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Özsoy, Çağla, Adriana L. Hotz, Nicolas N. Rieser, Zhenyue Chen, Xosé Luís Deán-Ben, Stephan C. F. Neuhauss, and Daniel Razansky. "Volumetric optoacoustic neurobehavioral tracking of epileptic seizures in freely-swimming zebrafish larvae." Frontiers in Molecular Neuroscience 15 (September 13, 2022). http://dx.doi.org/10.3389/fnmol.2022.1004518.

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Fast three-dimensional imaging of freely-swimming zebrafish is essential to understand the link between neuronal activity and behavioral changes during epileptic seizures. Studying the complex spatiotemporal patterns of neuronal activity at the whole-brain or -body level typically requires physical restraint, thus hindering the observation of unperturbed behavior. Here we report on real-time volumetric optoacoustic imaging of aberrant circular swimming activity and calcium transients in freely behaving zebrafish larvae, continuously covering their motion across an entire three-dimensional region. The high spatiotemporal resolution of the technique enables capturing ictal-like epileptic seizure events and quantifying their propagation speed, independently validated with simultaneous widefield fluorescence recordings. The work sets the stage for discerning functional interconnections between zebrafish behavior and neuronal activity for studying fundamental mechanisms of epilepsy and in vivo validation of treatment strategies.
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Yanny, Kyrollos, Nick Antipa, William Liberti, Sam Dehaeck, Kristina Monakhova, Fanglin Linda Liu, Konlin Shen, Ren Ng, and Laura Waller. "Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy." Light: Science & Applications 9, no. 1 (October 2, 2020). http://dx.doi.org/10.1038/s41377-020-00403-7.

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Abstract Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth range. Here, we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture stop. Placing the phase mask at the aperture stop significantly reduces the size of the device, and varying the focal lengths enables a uniform resolution across a wide depth range. The phase mask encodes the 3D fluorescence intensity into a single 2D measurement, and the 3D volume is recovered by solving a sparsity-constrained inverse problem. We provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the field-varying aberrations in miniature objectives. We demonstrate a prototype that is 17 mm tall and weighs 2.5 grams, achieving 2.76 μm lateral, and 15 μm axial resolution across most of the 900 × 700 × 390 μm3 volume at 40 volumes per second. The performance is validated experimentally on resolution targets, dynamic biological samples, and mouse brain tissue. Compared with existing miniature single-shot volume-capture implementations, our system is smaller and lighter and achieves a more than 2× better lateral and axial resolution throughout a 10× larger usable depth range. Our microscope design provides single-shot 3D imaging for applications where a compact platform matters, such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.
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Dissertations / Theses on the topic "Widefield motion"

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Evans, Bernard John Essex. "Neuronal encoding of natural imagery in dragonfly motion pathways." Thesis, 2019. http://hdl.handle.net/2440/120098.

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Vision is the primary sense of humans and most other animals. While the act of seeing seems easy, the neuronal architectures that underlie this ability are some of the most complex of the brain. Insects represent an excellent model for investigating how vision operates as they often lead rich visual lives while possessing relatively simple brains. Among insects, aerial predators such as the dragonfly face additional survival tasks. Not only must aerial predators successfully navigate three-dimensional visual environments, they must also be able to identify and track their prey. This task is made even more difficult due to the complexity of visual scenes that contain detail on all scales of magnification, making the job of the predator particularly challenging. Here I investigate the physiology of neurons accessible through tracts in the third neuropil of the optic lobe of the dragonfly. It is at this stage of processing that the first evidence of both wide-field motion and object detection emerges. My research extends the current understanding of two main pathways in the dragonfly visual system, the wide-field motion pathway and target-tracking pathway. While wide-field motion pathways have been studied in numerous insects, until now the dragonfly wide-field motion pathway remains unstudied. Investigation of this pathway has revealed properties, novel among insects, specifically the purely optical adaptation to motion at both high and low velocities through motion adaptation. Here I characterise these newly described neurons and investigate their adaptation properties. The dragonfly target-tracking pathway has been studied extensively, but most research has focussed on classical stimuli such as gratings and small black objects moving on white monitors. Here I extend previous research, which characterised the behaviour of target tracking neurons in cluttered environments, developing a paradigm to allow numerous properties of targets to be changed while still measuring tracking performance. I show that dragonfly neurons interact with clutter through the previously discovered selective attention system, treating cluttered scenes as collections of target-like features. I further show that this system uses the direction and speed of the target and background as one of the key parameters for tracking success. I also elucidate some additional properties of selective attention including the capacity to select for inhibitory targets or weakly salient features in preference to strongly excitatory ones. In collaboration with colleagues, I have also performed some limited modelling to demonstrate that a selective attention model, which includes switching best explains experimental data. Finally, I explore a mathematical model called divisive normalisation which may partially explain how neurons with large receptive fields can be used to re-establish target position information (lost in a position invariant system) through relatively simple integrations of multiple large receptive field neurons. In summary, my thesis provides a broad investigation into several questions about how dragonflies can function in natural environments. More broadly, my thesis addresses general questions about vision and how complicated visual tasks can be solved via clever strategies employed in neuronal systems and their modelled equivalents.
Thesis (Ph.D.) -- University of Adelaide, Adelaide Medical School, 2019
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Conference papers on the topic "Widefield motion"

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Shajkofci, Adrian, and Michael Liebling. "Estimating Nonplanar Flow From 2D Motion-Blurred Widefield Microscopy Images Via Deep Learning." In 2021 IEEE 18th International Symposium on Biomedical Imaging (ISBI). IEEE, 2021. http://dx.doi.org/10.1109/isbi48211.2021.9434156.

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Lee, Chau-Hwang, Chun-Chieh Wang, Chia-Wei Lee, Chia-Yun Huang, Jiunn-Yuan Lin, and Pei-Kuen Wei. "Three-dimensional motion of a nanoparticle on the cell membrane observed by non-interferometric widefield optical profilometry." In 2008 Conference on Lasers and Electro-Optics (CLEO). IEEE, 2008. http://dx.doi.org/10.1109/cleo.2008.4551890.

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