Academic literature on the topic 'Whole embryo culture'

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Journal articles on the topic "Whole embryo culture"

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Patat, Dilara, and Mehtap Nisari. "Hypoxia Modeling Technique in Whole Rat Embryo Culture." Ukraïnsʹkij žurnal medicini, bìologìï ta sportu 5, no. 3 (April 21, 2020): 82–88. http://dx.doi.org/10.26693/jmbs05.03.082.

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Van Maele-fabry, G., F. Gofflot, and J. J. Picard. "Whole embryo culture of presomitic mouse embryos." Toxicology in Vitro 9, no. 5 (October 1995): 671–75. http://dx.doi.org/10.1016/0887-2333(95)00064-f.

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Fujinaga, Masahiko, Richard I. Mazze, Jeffrey M. Baden, Alan G. Fantel, and Thomas H. Shepard. "Rat Whole Embryo Culture." Anesthesiology 69, no. 3 (September 1, 1988): 401–4. http://dx.doi.org/10.1097/00000542-198809000-00019.

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Fujinaga, M., R. I. Mazze, J. M. Baden, A. G. Fantel, and T. H. Shepard. "Rat Whole Embryo Culture." Obstetric Anesthesia Digest 9, no. 1 (April 1989): 23. http://dx.doi.org/10.1097/00132582-198904000-00024.

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Keyte, A. L., and K. K. Smith. "Monodelphis Whole-Embryo Culture." Cold Spring Harbor Protocols 2008, no. 11 (October 1, 2008): pdb.prot5075. http://dx.doi.org/10.1101/pdb.prot5075.

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Wlodarczyk, Bogdan, Bogumil Biernacki, Maria Minta, and Jan Zmudzki. "Postimplantation Whole Embryo Culture Assay for Hamsters: An Alternative to Rat and Mouse." Scientific World JOURNAL 1 (2001): 227–34. http://dx.doi.org/10.1100/tsw.2001.48.

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Postimplantation whole embryo culture (WEC) assay for rats and mice has been well established and introduced to many laboratories. Recently WEC technique for rabbits has been developed; however, information on culture of other species is very limited. Knowing the usefulness of hamsters in classical embryotoxicology, we reasoned that hamster WEC could be an alternative model for the most frequently used rat and mouse WEC. Previously we have optimized culture conditions for postimplantation hamster embryos. The aim of this study was to test the susceptibility of hamster embryos cultured in vitro to embryotoxic compounds and to compare our results with those reported by others on rat or mouse embryo culture. For that purpose we choose three known embryotoxic compounds�valproic acid, cadmium chloride, and diethylstilbestrol�and tested them using a postimplantation hamster whole embryo culture assay. Hamster embryos were cultured from 7.5 days gestation for 24 h in a medium consisting of 35% hamster serum and 65% synthetic culture medium (Iscove�s or McCoy 5A). At the end of the culture period, the embryos were examined morphologically, measured with the aid of a computer image analysis system, and total protein content was assessed. All three compounds exhibited dose-related embryotoxic and teratogenic effects in hamster embryos. The malformations observed were similar to those reported on rat and mouse embryos. Comparison of the results with data reported by other authors indicates that hamster embryos cultured in vitromight be more susceptible to embryotoxic stimuli than rat and mouse embryos.
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Steele, C. E. "Whole embryo culture and teratogenesis." Human Reproduction 6, no. 1 (January 1991): 144–47. http://dx.doi.org/10.1093/oxfordjournals.humrep.a137249.

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Steele, C. E., and R. Marlow. "Teratological studies using whole-embryo culture." Food and Chemical Toxicology 24, no. 6-7 (June 1986): 644. http://dx.doi.org/10.1016/0278-6915(86)90146-8.

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Piersma, A. H. "Whole embryo culture and toxicity testing." Toxicology in Vitro 7, no. 6 (November 1993): 763–68. http://dx.doi.org/10.1016/0887-2333(93)90079-k.

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Williams, Carolyn L., Paul K. Priscott, I. T. Oliver, and George C. T. Yeoh. "Albumin and transferrin synthesis in whole rat embryo cultures." Development 92, no. 1 (March 1, 1986): 33–41. http://dx.doi.org/10.1242/dev.92.1.33.

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The uptake of [3H]leucine by the rat yolk sac and embryo and the subsequent synthesis of albumin and transferrin have been studied in whole embryo culture. Rat embryos of 12 days gestation were used in all experiments. Isotopically labelled transferrin was detectable in yolksac and embryo tissue extracts. In contrast, [3H]albumin could not be found in either tissue extract. Levels of radioactive transferrin in the yolk sac of cultured whole conceptuses decreased during 12 h in cold media. Embryonic transferrin showed an opposite trend in that it increased over 12 h by nearly 30-fold. In view of these results experiments were conducted in embryos and yolk sacs cultured in separate bottles. Radioimmunoprecipitation for transferrin revealed that there was synthesized protein in the yolk sac which then decreased by approximately 30% after 2h in normal cultured medium. There was no evidence of transferrin synthesis in embryo extracts over a 12 h period. These results present evidence that the visceral yolk sac is the primary site of transferrin synthesis in the rat and that the protein is thereafter transported, intact, to the embryo.
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Dissertations / Theses on the topic "Whole embryo culture"

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Zeeb, Martin [Verfasser], Eckhard [Akademischer Betreuer] Lammert, and Christopher R. [Akademischer Betreuer] Bridges. "Whole-embryo culture of mouse embryos to study vascular development / Martin Zeeb. Gutachter: Christopher R. Bridges. Betreuer: Eckhard Lammert." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2013. http://d-nb.info/1033639990/34.

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Xu, Jian, and 徐堅. "Using antisense oligonucleotide in whole embryo culture to study gene interactions during mouse gastrulation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31220150.

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Xu, Jian. "Using antisense oligonucleotide in whole embryo culture to study gene interactions during mouse gastrulation /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19918884.

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Bücken, Andreas. "Optimierung der Whole-Embryo-Culture von 9,5 Tage alten Rattenembryonen durch Austestung verschiedener Pufferlösungen sowie durch Einführung eines kontinuierlichen Begasungsverfahrens (Rotator-System) Verlängerung der Kulturdauer auf 72 (96) Stunden /." Berlin : Mensch-und-Buch-Verl, 2006. http://www.diss.fu-berlin.de/2006/444/index.html.

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Anwar, Mohammad. "An investigation of the teratogenic properties of human sera using a mammalian whole embryo culture technique." Thesis, University of Leicester, 1986. http://hdl.handle.net/2381/35083.

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Using the rodent embryo culture techniques of New (1978), it can be demonstrated that, over the 48-hours culture period, the in-vitro growth and development, of explanted 9.5 days old post-implantation rat conceptuses, in immediately-centrifuged and heat-inactivated homologous serum is almost indistinguishable from that which occurs in-vivo up-to 11.5 days. Moreover, using these methods, it has become possible to grow rat conceptuses in a culture medium made up of 90 per cent human serum supplemented with glucose and 10 per cent rat serum. Embryonic growth and developmental indices achieved in culture, using supplemented human serum, were reported to be comparable to those observed in whole rat serum (Reti et al., 1982). The successful growth of rat conceptuses during the major part of the organogenetic stage, is an important technical departure, since it enables the effect of potential teratogens, in human serum to be investigated. In order to validate the current embryo culture model, it was demonstrated initially that reproducible embryonic growth and development can be achieved in appropriately prepared serum samples, obtained from healthy women during the various stages of their menstrual cycles, pregnancy and the post-natal period. Furthermore, using serum from patients with ovarian malignancies, on antimitotic chemotherapy, it was demonstrated that the modified whole rat embryo culture model is highly sensitive in distinguishing between normal and embryotoxic human sera. This method of maintaining embryos in culture, was adapted to investigate the differences in the species specific embryopathic properties of valproic acid. This anticonvulsant agent, commonly used during pregnancy, was found to be embryopathic at a lower serum concentration in rats than in humans. The implications of these findings are discussed with a view to investigate potential embryotoxicity of drugs used during human pregnancy. Finally, sera from women with histories of neural tube defect (NTDS) affected pregnancies and those with unexplained recurrent miscarriages were used as a culture medium to investigate serum related environmental embryopathic factors. Experimental work reported in this thesis indicates, that culture of rat conceptuses in appropriately prepared sera from 76 per cent of women with NTD affected pregnancies and 25 per cent of the habitual aborters resulted in an abnormal growth.
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Dinort, Kathrin [Verfasser]. "Untersuchung von N-Methyl-pyrrolidon und seinen Metaboliten auf Embryotoxizität in der `Whole Embryo Culture´ / Kathrin Dinort." Berlin : Freie Universität Berlin, 2007. http://d-nb.info/1022457292/34.

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Kamijo, Hiroshi. "Impaired vascular remodeling in the yolk sac of embryos deficient in ROCK-I and ROCK-II." Kyoto University, 2015. http://hdl.handle.net/2433/199189.

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Pock, Tim Verfasser], and Verena [Akademischer Betreuer] [Nordhoff. "The effect of growth factor supplemented ART culture media on whole mouse embryo development / Tim Pock ; Betreuer: Verena Nordhoff." Münster : Universitäts- und Landesbibliothek Münster, 2019. http://d-nb.info/1197692002/34.

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Pock, Tim [Verfasser], and Verena [Akademischer Betreuer] Nordhoff. "The effect of growth factor supplemented ART culture media on whole mouse embryo development / Tim Pock ; Betreuer: Verena Nordhoff." Münster : Universitäts- und Landesbibliothek Münster, 2019. http://d-nb.info/1197692002/34.

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Kilb, Caroline [Verfasser]. "Untersuchung von fünf Fremdstoffen und deren Hauptmetaboliten allein und in Kombination in der Whole Embryo Culture mit Rattenembryonen / Caroline Kilb." Berlin : Freie Universität Berlin, 2013. http://d-nb.info/1031100954/34.

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Books on the topic "Whole embryo culture"

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Development toxicity of methanol in whole embryo culture: A comparative study with mouse and rat embryos. [Washington, D.C.?: Environmental Protection Agency], 1993.

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Taiz, Lincoln, and Lee Taiz. Flora’s Secret Gardens. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780190490263.003.0018.

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Wilhelm Hofmeister established the unity of the Plant Kingdom through the discovery of Alternation of Generations. In both cryptogams and flowering plants a diploid asexual stage, or sporophyte, alternates with a haploid sexual stage. Thus the flower is not the true sexual stage, but rather the asexual spore-producing stage. The main difference between ferns and roses is that the spores of the fern are visible on the undersides of the leaves, while the spores of the rose are concealed within the anthers and ovaries. These spores develop into the actual sexual stage of the spermatophyte, the male and female gametophytes, i.e. the pollen tube and the embryo sac. Hofmeister’s discovery solved of the age-old quandary over plant sex. The sexualists and the asexualists can both claim to have been correct, but it was the sexualists who freed their minds from cultural biases and glimpsed the true sexual nature of plants.
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Book chapters on the topic "Whole embryo culture"

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Harris, Craig. "Rodent Whole Embryo Culture." In Methods in Molecular Biology, 215–37. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-867-2_13.

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Marshall, Valerie A., and Edward W. Carney. "Rabbit Whole Embryo Culture." In Methods in Molecular Biology, 239–52. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-867-2_14.

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Tung, Emily W. Y., and Louise M. Winn. "Mouse Whole Embryo Culture." In Methods in Molecular Biology, 187–94. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9182-2_13.

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Harris, Craig. "Rat Whole Embryo Culture." In Methods in Molecular Biology, 195–217. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9182-2_14.

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Ozolinš, Terence R. S. "Rabbit Whole Embryo Culture." In Methods in Molecular Biology, 219–33. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9182-2_15.

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Pryor, Sophie E., Valentina Massa, Dawn Savery, Nicholas D. E. Greene, and Andrew J. Copp. "Convergent Extension Analysis in Mouse Whole Embryo Culture." In Methods in Molecular Biology, 133–46. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-510-7_11.

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Hang, Calvin T., and Ching-Pin Chang. "Use of Whole Embryo Culture for Studying Heart Development." In Methods in Molecular Biology, 3–9. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-523-7_1.

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Liang, Bo, and Chi Chiu Wang. "Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs." In Methods in Molecular Biology, 177–89. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7883-0_8.

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Zhang, Cindy, Julie Panzica-Kelly, and Karen Augustine-Rauch. "The Rat Whole Embryo Culture Assay Using the Dysmorphology Score System." In Methods in Molecular Biology, 423–50. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-131-8_30.

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Steele, C. E., and R. Marlow. "The Use of Whole Embryo Culture in the Study of Teratogenic Mechanisms." In Archives of Toxicology, 432–33. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-69928-3_97.

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Conference papers on the topic "Whole embryo culture"

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TSUNEKAWA, YUJI, MASANORI TAKAHASHI, and NORIKO OSUMI. "LABELING OF NEUROEPITHELIAL CELLS USING WHOLE EMBRYO CULTURE AND GENE TRANSFER METHODS TO CHARACTERIZE THE CELL CYCLE." In Proceedings of the Final Symposium of the Tohoku University 21st Century Center of Excellence Program. IMPERIAL COLLEGE PRESS, 2006. http://dx.doi.org/10.1142/9781860948800_0023.

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Kuzmina, T. I., D. N. Tatarskaya, and V. YU Kravtsov. "PROSPECTS FOR THE USING OF SILICON-CONTAINING COMPOUNDS IN THE TECHNOLOGY OF IN VITRO MATURATION OF ANIMAL OOCYTES." In "International Scientific and Practical Conference" THEORY AND PRACTICE OF VETERINARY PHARMACY, ECOLOGY AND TOXICOLOGY IN AIC ", dedicated to the centenary of the Department of Pharmacology and Toxicology, SPbSUVM. FSBEI HE St. Petersburg SUVM, 2021. http://dx.doi.org/10.52419/3006-2021-2-129-131.

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In vitro production of animal embryos is an important tool for solving male and female infertility problems in animals. Modeling of extracorporeal maturation systems of oocytes using siliconcontaining compounds in the composition of culture media revealed the peculiarities of the realization of their effects on somatic and germ cells of ovarian follicles, depending on the structure. Highly dispersed silica nanoparticles (nHDS) positive effects on the fertility of female gametes in Bos Taurus and Sus Scrofa Domesticus. The gel substrate of silica (silicon dimethylglycerolate - DMGC) does not cause an increase in the level of embryos cleavage, while it does not have cytoand genotoxicity when tested on somatic and germ cells of antral follicles.Key words: siliconcontaining compounds, in vitro maturation, animal oocytes.
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Wan, Chen-rei, Seok Chung, Ryo Sudo, and Roger D. Kamm. "Induction of Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells in a Confined Microfluidic Environment." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-203995.

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Embryonic stem cell derived cardiomyocytes are deemed an attractive treatment option for myocardial infarction. Their clinical efficacy, however, has not been unequivocally demonstrated. There is a need for better understanding and characterization of the cardiogenesis process. A microfluidic platform in vitro is used to dissect and better understand the differentiation process. Through this study, we find that while embryoid bodies (EBs) flatten out in a well plate system, differentiated EBs self-assemble into complex 3D structures. The beating regions of EBs are also different. Most beating areas are observed in a ring pattern on 2D well plates around the center, self-assembled beating large 3D aggregates are found in microfluidic devices. Furthermore, inspired by the natural mechanical environment of the heart, we applied uniaxial cyclic mechanical stretch to EBs. Results suggest that prolonged mechanical stimulation acts as a negative regulator of cardiogenesis. From this study, we conclude that the culture environments can influence differentiation of embryonic stem cells into cardiomycytes, and that the use of microfluidic systems can provide new insights into the differentiation process.
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