Dissertations / Theses on the topic 'Wfdb'

Обсяг роботи 109 сторінок, кількість ілюстрацій – 29, таблиць – 30, додатків – 1, джерел за переліком посилань – 50. Мета дослідження – розробка пакета програм візуалізації та напівавтоматичної розмітки електрокардіографічних даних, для підвищення ефективності систем автоматичного аналізу ЕКГ та серцевого ритму. Об’єкт дослідження – медичні показники ЕКГ та серцевого ритму за ресурсом PhysioNet. Предметом дослідження є розробка програми, що дозволяє взаємодіяти з фізіологічними сигналами формату PysioNet. Додаток дозволяє переглядати сигнали, анотації до них, а також скачувати потрібні записи при підключенні, до мережі Інтернет. Програма здійснює перетворення, сигналу з формату EDF та CSV формату PhysioNet. Для створення додатка використовується мова програмування Python. Як середовище програмування вибрана Jupiter Notebook. Для взаємодії з сигналами до проекту підключена бібліотека Waveform Database Library. У роботі є короткий огляд методів електрокардіографії, мови Python і платформи PysioNet, так як всі ці складові були використанні при розробці додатку. Публікація: 1. Солодкий В. П. Програмний додаток для візуалізації медичних показників за ресурсом PhysioNet. / Солодкий В.П. // Міжнародний науковий журнал "Інтернаука". — 2019. — №6. — 78 — 86 С. (стаття) Розроблена програма дозволяє виконувати всі поставлені функції. Але відомі шляхи поліпшення і модифікації програми. Наприклад, можливість шукати та скачувати медичні засоби за певними показниками з анотацій.
Volume of pages - 109, number of illustrations - 30, tables - 30, applications - 1 , sources - 50. The purpose of the study is to develop a package of visualization programs and semi-automatic marking of electrocardiographic data to improve the efficiency of ECG and heart rate systems. The object of the study is the medical parameters of ECG and cardiac rhythm with the PhysioNet resource. The subject of the study is the development of a program that allows you to interact with the physiological signals of the PysioNet format. The application allows you to view signals, annotations to them, and download the required entries when connected to the Internet. The program converts the signal from the EDF format and the CSV format to PhysioNet. The Python programming language was used to create the application. Jupiter Notebook was choose as the programming environment. The Waveform Database Library library was connected to the project to interact with the signals. There is a brief overview of electrocardiography, Python and PysioNet, as all of these components were used in the development of the application. Publication: 1. Solodky V.P. Software application for visualization of medical indicators with the resource PhysioNet. / Solodkyi V.P. // International scientific magazine "Internet Science". — 2019 — №6. — 78 — 86 P. (article) The developed program allows you to perform all the functions assigned. However, there are ways to improve and modify the program. For example, the ability to search and download medical devices for certain indicators of annotations.

SLPI and elafin are members of the whey acidic protein (WAP) four disulphide core (WFDC} family of proteins and have multiple contributions to innate host defence including inhibition of neutrophil serine proteases, antimicrobial properties and inhibition of the inflammatory response to LPS. This research aimed to investigate novel functions of WAP2 and eppin, two lesser characterised WFDC proteins that are found expressed in the lung. Recombinant expression and purification of WAP2 were first optimised in E. coli for use in a range of functional assays. Recombinant WAP2 inhibited cathepsin G protease activity. Monocytic cells pre-treated with recombinant WAP2 before LPS stimulation produced significantly lower levels of IL-8 and MCP-l compared to cells stimulated with LPS alone. Recombinant WAP2 became conjugated to fibronectin in a transglutaminase-mediated reaction and retained antiprotease activity. WAP2 was detected at variable levels in bronchoalveolar lavage fluid from cystic fibrosis patients. WAP2 and eppin were both shown by immunohistochemistry to be expressed in epithelial and immune cells of the lung. Eppin exerted potent antibacterial effects against the lung-colonising bacteria P. aeruginosa and S. aureus, in contrast to WAP2 which was ineffective. Together these results suggest a role for both WAP2 and eppin in the innate host response. Further characterisation is required to fully understand the range and implications of their physiological functions.

Orientador: Erick José Ramo da Silva
Resumo: O epidídimo é responsável por transformar os espermatozoides imaturos em células funcionalmente competentes, capazes de adquirir motilidade progressiva e de reconhecer e fertilizar o oócito. Além disso, o epidídimo é primordial para proteger e armazenar o gameta masculino antes da ejaculação. Alterações da fisiologia epididimária podem afetar a sua função, causando infertilidade masculina. A inflamação do epidídimo, conhecida como epididimite, é uma das doenças mais prevalentes do trato urogenital masculino, sendo um fator relevante de infertilidade masculina. Sua principal etiologia envolve a invasão de bactérias, como Escherichia coli, via ascensão retrógrada pela uretra. O epidídimo expressa constitutivamente diversos componentes do sistema imunológico inato, incluindo os receptores do tipo Toll (TLRs), como o TLR4, que é ativado pelo lipopolissacarídeo (LPS) de bactérias Gram-negativas, além de proteínas com atividade antimicrobiana, como os inibidores da protease do tipo WFDC (Whey-acidic protein four disufide core), indicando que este órgão está em constante estado de alerta para combater infecções bacterianas. Além da ação antimicrobiana e inibidora de protease, as proteínas WFDC apresentam ação anti-inflamatória, imunomoduladora e sobre a função espermática, sendo proteínas multifuncionais no trato reprodutor masculino. Neste estudo, testamos a hipótese de que a expressão de genes Wfdc no epidídimo é modificada por estímulos inflamatórios, como parte da resposta tecid... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The epididymis is responsible for transforming immature sperm into functionally competent cells capable of acquiring progressive motility and recognizing and fertilizing the oocyte. In addition, the epididymis is paramount for protecting and storing the male gamete before ejaculation. Changes in epididymal physiology may affect sperm maturation, transport or storage, causing male infertility. Epididymal inflammation, known as epididymitis, is one of the most prevalent diseases of the male urogenital tract, being a relevant factor of male infertility. Its main etiology involves the invasion of bacteria, such as E. coli, through retrograde ascension through the urethra. The epididymis constitutively expresses several elements of the innate immune system, including Toll-like receptors (TLRs), like the TLR4, which is activated by the lipopolysaccharide (LPS) of Gram-negative bacteria, as well as proteins with antimicrobial activity, like the WFDC (Whey-acidic difulfide core)-type protease inhibitors, indicating that this organ is constantly alert to fight bacterial infections. In addition to antimicrobial and protease inhibitory activities, WFDC proteins show anti-inflammatory, immunomodulatory and reproductive functions, being multifunctional proteins in the male reproductive tract. In this study, we tested the hypothesis that Wfdc gene expression in the epididymis is modified by inflammatory stimuli as part of tissue defense mechanisms to microbial aggression. For that, we char... (Complete abstract click electronic access below)
Mestre

Diplomová práce popisuje metodiku výpočtů částí náchylných k rozvoji mnoho-ohniskového únavového poškození (WFD). Nejdříve se práce zabývá rozborem předpisů letové způsobilosti souvisejících s WFD pro letadla certifikovaných podle FAR 23 v kategorii commuter. Následuje popis metodiky únavového výpočtu a predikce šíření trhliny. Dále jsou popsány řešení pro výpočet faktoru intenzity napětí pro základné konfigurace trhliny, tam kde to bylo možné v uzavřeném tvaru, jinak ve formě tabulky. Hlavní částí práce je návrh algoritmu AIMA schopného provést kompletní analýzu části náchylné k rozvoji únavového poškození na více místech (MSD). Model se validoval únavovou zkouškou vzorku přeplátovaného spoje dolního panelu křídla. Následně byla analyzována reálná konstrukce spoje dolních panelů křídla letounu L 410 NG.

Natural Resource Management processes are undergoing major transformations: technical and regulatory mechanisms are no longer considered sufficiently adaptive to address the complexity and uncertainty which characterise contemporary challenges in the sector, thus motivating wider use of integrated and collaborative approaches. Against this background, new models of participative management are encouraged which emphasise social learning among stakeholders. Yet, reported research which unambiguously demonstrates the role and impact of social learning remains sparse. This thesis contributes to a better understanding of the conditions under which social learning occurs, and most importantly the dynamics and benefits of social learning by systematically collecting evidence of the processes and impacts attributed to social learning. The research which employs a sequential mixed methods research design is undertaken with stakeholders involved in various engagement activities forming part of the implementation of the WFD in the UK, Ireland, and Germany and expands the still limited empirical knowledge base on social leaning in stakeholder interaction. Findings demonstrate that participatory platforms are shaped by processes of social learning although they are more noticeable as collaborative initiatives mature. Also, there is some degree of variation in the extent to which people learn or change, with stakeholders readily acquiring knowledge and improving relationships. However, the transformation of views and the development of a shared group identity seem to be limited. Findings clearly illustrate the multitude of factors that constrain the occurrence of learning processes and eventually limit the extent to which these can contribute to sustainable NRM. Foremost, this study reinforces the importance of the actual communicative learning process, the quality and intensity of which is largely influenced by the organisational arrangements and, more fundamentally, the ability of the stakeholders to shape the process.

Of all natural resources, water is particularly under pressure due to its intensive use. Therefore, it is vital to better understand the decision-making processes that could reverse trends in environmental deterioration. Policy appraisal tools can help decision makers develop sustainable public policies, as they support evidence-based policy choices. In practice, however, they are rarely used as a basis for decisions. In this context, this PhD has aimed to answer the following research question: How can we explain the different uses of policy appraisal - in particular of economic analysis – beyond evidence-based policy making in water decision making? To this end, I studied the case of the EU Water Framework Directive (WFD) (2000/60/EC) and the use of cost-benefit analysis in its application in the United Kingdom and France, as recent examples in which economic-based policy appraisal plays, in principle, a key role. I showed that appraisal tools could reflect political objectives and environmental policy ambitions in two ways. Firstly in the choice of the appraisal tool itself, which follows a specific logic. Secondly, the operationalisation of the tool selected may be influenced by political goals. As a consequence, the use of policy appraisal for evidence-based decision making can partly be explained by the discrepancy between the output of the appraisal and the political objectives pursued. More generally, I found that the politics stream – i.e. the political context - determines the presence and importance of appraisal tools in the policy process and explains further uses, in particular in problem definition and policy formulation. I also provided an empirical contribution to the knowledge on WFD implementation in member states. I explained the process of setting objectives and exemptions in two member states, and the role that economic analysis played in the decision-making process. I showed that ambitions related to the implementation of the WFD, and the political context more generally, shaped the decisions on the analytical tools used and that choices made in the operationalisation of these tools partly influenced the protection standards of individual water bodies. These results imply that debates on the use of policy appraisal in WFD implementation should go beyond experts’ circles and be considered in the political sphere.

Gli ecosistemi acquatici sono il comparto ambientale finale per molti contaminanti introdotti nell’ambiente, specialmente per quelli persistenti, provenienti da diverse attività antropiche. Gli organismi acquatici sono esposti simultaneamente a differenti classi di composti chimici e, la loro fisiologia può esserne affetta anche da basse concentrazioni ambientali che ne determinano un effetto a cascata drammatico sull’intero ciclo vitale delle stesse. Per identificare gli effetti ecotossicologici si devono scegliere delle variabili di studio che sono sensibili a cambiamenti di esposizione e quindi possono registrare informazioni a differenti livelli di organizzazione biologica (ad es.: comunità, specie, alterazioni cellulari, molecolari). Il presente lavoro ha come scopo di studiare biomarker biochimici e bioindicatori su invertebrati acquatici capaci di rispondere all’effetto dei contaminanti e predirne il rischio di tossicità, requisito fondamentale dell’attuale legislazione comunitaria ambientale. Questo lavoro, nell’ambito della Water Frame Directive (WFD), affronta questa tematica con diversi tipi di approccio per implementare un sistema di monitorizzazione efficiente. Lo SPEAR ad esempio è in grado di diagnosticare la contaminazione di ecosistemi acquatici da pesticidi operando a livello di comunità di invertebrati acquatici. Inoltre si sono presi in considerazione anche biomarker a livello cellulare in bivalvi (Tapes philipphinarum and Mya arenaria): attività enzimatiche coinvolti nelle risposte aerobiche (CCO) e antiossidative (SOD and CAT), risposte genotossicologiche come la frequenza dei micronuclei, e, infine, a livello di organismo, risposte di carattere comportamentale.
Aquatic ecosystems are the final sink for most of contaminants introduced in the environment, especially persistent organic pollutants coming from different human activities. Most of the freshwater and coastal organisms are exposed simultaneously to different classes of chemicals, and their physiology may be affected by toxic chemical even at low environmental concentrations with a cascade event on population dynamics. Identifying ecotoxicological effects requires descriptors that are sensitive to changes in exposure and thus can indicate information at different levels of biological organisation (i.e. communities, sub-individual level). The present work aims to investigate biochemical biomarkers and bioindicators in aquatic invertebrates able to detect biological effects and predict risk of toxicity, with a special focus paid to the requirements of current EU regulation. Therefore, the present work faced on different kinds of approach identified to be efficient tools within the context of the WFD requirements (Water Frame Directive, 2000/60/EC), for investigative and operational monitoring. Work include the SPEAR approach as trait based indicator descriptor of freshwater aquatic macroinvertebrates communities, able to diagnose pesticide contamination in aquatic ecosystems. Further diagnostic approaches in environmental status are included, as assay representing biological responses for different enzymes involved in the aerobic (CCO) and antioxidant responses, (SOD, and CAT), behavioural endpoints, and genotoxicity biomarker associated with micronuclei frequency in bivalve species (Tapes philipphinarum and Mya arenaria).

Increased wildfire activity over the last years has made fire managers to become more concerned about the effectiveness of current fuel treatment practices to reduce fire risk and severity. Fuel treatments lead to changes in the forest structure and therefore modify certain basic parameters that may influence the fire behaviour. The goal of this work is to quantitatively assess the effectiveness of fuel treatments in reducing risk of wildfires spread in some typically Mediterranean forest. Specifically, it envisages to know the characteristics of the fire that can occur in the slash originated from regenerated aleppo pine stands that were thinned after a wildfire and also to know the evolution of slash combustibility after the treatment. First, an extensive literature review has been done, focused mainly on those works that assessed the effectiveness of fuel treatments in reducing the risk of wildfire. Several studies demonstrated the effectiveness of treatments such as prescribed burning or thinning. However, these studies are not consistent about what treatment and slash management technique are the most effective. A study area has been selected and located within the perimeter of a large forest fire occurred in Bages (Central Catalonia, Spain) in 1994 to study the effectiveness of a type of fuel treatment usually performed in the Mediterranean Basin. The site was previously thinned during different periods and the slash was left in, not managed. In this area, the fuel, environment and topographic characteristics have been determined. With the fuel sampling methods used at the site, a protocol has been produced in order to characterize the regenerated aleppo pine stands, useful to obtain the parameters needed to evaluate fire behaviour. Results have led to a new fuel model for regenerated aleppo pine stands and also fuel models for regenerated aleppo pine stands that have been thinned at different times with the slash remaining unmanaged at the site. An experimental burning program has been implemented reproducing two different regenerated structures (6 and 40 months after treatment). With these experiments it has been proved that ex-situ experimentation is not useful to reproduce actual fire situations because it is impossible to repeat faithfully the overall structure of the ecosystem that affect fire behavior. A series of simulations of potential fires that could occur in the study area have been performed, incorporating the determined inputs (environmental conditions and fuel type) and evaluating different simulation tools (NEXUS i WFDS). Results obtained with the two simulations tools used confirm that both tools are reliable. In general values obtained in fireline intensity and rate of spread with WFDS are larger than with NEXUS. Results from simulations of Bages plots carried with both simulation tools generally show that fire behavior is more severe 1 month and 6 months after the treatment. That is, the wildfire effects after the treatment would be worse than in the untreated control plot and that time does not improve this situation, at least six months after treatment. According to the results and given the characteristics of our country where the risk of fires is very high, the effect of thinning treatments performed in central Catalonia after the 1994 fire brings a great fire vulnerability in treated stands, at least during the first months of treatment.

Successfulwater management implies tackling multi-level governance and improvingintegration between sectors. Sound information and related processes will berequired to support water management decision-making at these various levels.Additionally, considering Principle 10 of the UN’s Agenda 21, actors shouldhave access to information to enable their involvement in shaping watermanagement outcomes. This thesis draws on the results of two separate caseswhere information systems support action: (i) rural water supply in Uganda;and, (ii) water management according to the Water Framework Directive in Sweden.A research approach was formed based on: (a) a model conceptualising how informationsystems support organisational processes which lead to action; and, (b) asystems-thinking methodology. The results reveal that there are numerous,similar challenges to achieving information support for action in both theUgandan and Swedish cases. In both cases, information quantity and quality islimited; consequentially, the use of information to support action is inhibited.Furthermore, not all actors are involved in information system processes; in particular,local-level actors. Overall, there is limited support of strategic decision-makingand weak support of operational, or local, decision-making. The results suggestthat it might be possible to tailor strategic-level information processes tolocal needs, hopefully encouraging active involvement of local actors. Improvedinvolvement, together with a suitable systems approach, could be used to furtherdevelop information systems, improving integration between multiple levels ofgovernance and across sectors – suiting not just the needs ofstrategic decision-making but also the needs of operational, or local,decision-making.
QC 20120822

Les milieux côtiers subissent de fortes pressions dues aux activités anthropiques. Ces pressions vont parfois impacter les écosystèmes benthiques. En Europe, des réglementations internationales, telle que la Directive Cadre sur l’Eau (DCE), ont vu le jour. La DCE a fixé un cadre pour obtenir le retour et le maintien d’un bon état écologique pour les masses d’eau côtières, jusqu’à un mile de la côte. Pour vérifier si ce bon état écologique est atteint, des outils de mesure adaptés et performants sont nécessaires. L’utilisation d’indices biotiques basés sur les faunes de foraminifères benthiques est un des moyens pour mesurer l’état des écosystèmes. Ces indices basés sur les faunes de foraminifères sont en pleine expansion, notamment en Méditerranée. En termes de méthodologie, nous avons pu améliorer une technique de concentration des foraminifères benthiques par séparation par densité, qui accélère le traitement des échantillons. L’indice TSI-Med a la particularité d’apporter une correction pour l’état trophique naturel, c’est-à-dire le taux d’enrichissement naturel en matière organique, sur la base de la granulométrie du sédiment. Nos données suggèrent néanmoins que cette correction est encore insuffisante. Nous avons également comparé plusieurs indices biotiques basés sur les faunes de foraminifères benthiques le long des côtes méditerranéennes françaises. Les indices basés sur la diversité, notamment l’exp(H’bc), ne sont pas adaptés à notre zone d’étude oligotrophe. Les indices basés sur les groupes écologiques d’espèces que nous avons testés (TSI-Med, Foram-AMBI et FSI) sont plutôt bien corrélés. Le principal facteur qui va induire des différences entre ces indices est la liste d’assignation écologique des espèces. Trop d’espèces n’ont pas encore été assignées et l’assignation de plusieurs espèces majeures est problématique. Néanmoins, nous avons montré que ces indices sont efficaces à détecter l’impact d’un enrichissement en matière organique diffus et/ou provenant de source ponctuelle, telle que les rejets de stations d’épuration (côte israélienne). Pour perfectionner ces indices, des efforts de caractérisation écologique des espèces doivent être poursuivis
Coastal environments suffer from anthropogenic activities. Various types of pressure can have large impacts on benthic ecosystems. In Europe, international regulations, such as the Water Framework Directive (WFD), have been implemented. The WFD aims to attain and sustain a good ecological status for all coastal waters, up to one mile from the coast. To verify whether this good status has been reached, adapted and efficient measuring tools are needed. The use of biotic indices based on foraminiferal faunas are a mean to assess the ecosystem status. The use of indices based on foraminiferal faunas rapidly increases, especially in the Mediterranean. In terms of methodology, we were able to improve a method to concentrate benthic foraminifera by density separation, which accelerates the sample treatment. The TSI-Med index includes a correction to take into account the natural trophic state, i.e., the natural enrichment rate in organic matter, on the basis of sediment grain size. However, our data suggest that the applied correction is still insufficient. We also compared several biotic indices based on benthic foraminiferal faunas along the French Mediterranean coast. Indices based on diversity, such as the exp(H’bc), are not suited for our oligotrophic study area. The results of three tested indices based on ecological groups (TSI-Med, Foram-AMBI, FSI) are well correlated. The main factor inducing differences between the indices is the list of ecological assignments for the various species. In general, too many species have not yet been assigned to ecological categories and the assignment of several major species is problematic. Nevertheless, we show that the indices are already efficient in detecting the impact of diffusive organic matter enrichment or enrichment from point sources, such as sewage outlets (Israelian coast). To further perfection the foraminiferal indices, the efforts of ecological assignments of species have to be continued

L’identification des bases moléculaires de la tolérance au gel présente une grande importance tant sur le plan fondamental qu’appliqué. Medicago truncatula est une légumineuse modèle pour les espèces tempérées. Un QTL majeur de tolérance au gel après acclimatation au froid (Mt-FTQTL6), expliquant 40% de la variation phénotypique, a été détecté sur le chromosome 6 de cette espèce. En parallèle, un QTL pour le même caractère (WFD 6.1/FD164.c) a été identifié sur le groupe de liaison équivalent chez Pisum sativum. Cette thèse a pour objectifs de confirmer la synténie des régions chromosomiques contenant Mt-FTQTL6 et WFD 6.1/FD164.c et d’identifier des gènes candidats positionnels pour Mt-FTQTL6. Les premiers efforts ont permis de localiser Mt-FTQTL6 dans un intervalle de 3,7 cM qui coïncide avec une zone du génome de Medicago truncatula dont l’assemblage reste incomplet. Les ressources génomiques de Glycine max ont été ensuite exploitées. Cinq marqueurs géniques ont permis d'ancrer les régions chromosomiques de Mt-FTQTL6 et WFD 6.1/FD164.c. Des clones BAC correspondant à 15 marqueurs (sondes) ont été assemblés en 6 contigs couvrant l’intervalle de confiance de Mt-FTQTL6. Des lignées F7 ou F8, recombinantes au niveau de cet intervalle, ont été identifiées et phénotypées pour la tolérance au gel en conditions contrôlées. L'intervalle de confiance de Mt-FTQTL6 a ainsi été réduit à une région de 0,4 cM contenant 20 gènes parmi lesquels 12 gènes CBF/DREB1 en tandem. La variation allélique pour 11 gènes CBF/DREB1 a été mise en évidence chez les parents de la population de cartographie.La validation fonctionnelle est maintenant envisageable chez Medicago truncatula et Pisum sativum
Unraveling the molecular bases of freezing tolerance is of great importance both at the fundamental and applied levels. Medicago truncatula is a model legume for studies concerning cool-season species. A major freezing tolerance QTL after cold acclimation (Mt-FTQTL6), accounting for 40% of the phenotypic variation, has been identified on chromosome 6 of this species. Interestingly, a QTL for the same trait has been mapped on the corresponding linkage group in Pisum sativum (WFD 6.1/FD164.c). The present thesis aimed to confirm synteny between Mt-FTQTL6 and WFD 6.1/FD164.c harboring regions and to subsequently identify positional candidate genes for Mt-FTQTL6. Using BAC-derived markers, Mt-FTQTL6 has been first located in a 3.7-cM interval, coinciding with an assembly physical gap. Mt-FTQTL6 co-orthologous blocks in Glycine max were identified and exploited to develop additional markers. Five common gene-based markers were obtained between Mt-FTQTL6 and WFD 6.1/FD164.c chromosomal regions. Positive BAC clones for 15 different markers (probes) were assembled in 6 BAC contigs linked to Mt-FTQTL6. Homozygous F7 or F8 recombinant lines at Mt-FTQTL6 were identified and evaluated for freezing tolerance under controlled conditions. The QTL confidence interval was subsequently delimited to a 0.4 cM-region that contains 20 protein-coding genes including 12 tandemly-arrayed CBF/DREB1 genes. Isolation of 11 out of the 12 CBF/DREB1 genes from both parents of the mapping population was successfully achieved. Efforts will be next needed for functional validation in Medicago truncatula and Pisum sativum

The thesis studies one method, focus group discussions, for public participation in the starting of implementing the European Community Water Framework Directive at a catchment level. Focus group discussions can be used as a method for bringing relevant stakeholder-groups into the discussion and evaluation of different possible solutions to problems of managing local waters in order to lower nutrient emissions and stop eutrophication. Of special interest in the study is the participants’ development of collaboration and collaborative learning in the focus groups. Also of interest is their change in attitudes during the focus group process and their will to participate in similar settings. The thesis assesses the results of three questionnaires responded by focus group participants in a study carried out by the Swedish Water Management Research Programme, VASTRA. The participants represent stakeholders in Rönne å catchment in Skåne, and also the results from ten focus group discussions with the same participants.

Management of water bodies according to their river basinsis becoming increasingly common, as the concept of IntegratedWater Resources Management (IWRM) is getting more and moreacknowl-edged. In Europe, a large portion of these“new”management units will probably be internationalor transboundary. Decisions and policies consideringtransboundary water issues need to be based on reliable andcomprehendible information. In this thesis, a review of variousexisting models that may be used for understanding the role anduse of information in (transboundary) water management ispresented. Further, the thesis reports on an assessment of theinformation management of three transboundary water regimes inEurope, namely the international water commissions for LakeNeusiedl, Lake Constance and Elbe River. Besides examining theinformation management of the regimes as such, the managementwas also related to information needs implied by the IWRMcon-cept and by the EU Water Framework Directive (WFD). Thereviewed models and approaches were grouped into threecategories: information management models, information cyclemodels and communication between actors. The first categorycomprised models that may be used for managing and assessingdifferent types of information. The second group dealt withmodels explaining the production and communication ofinformation predominately from an information producer/senderperspective. The third group focused on ideas concerninginteractions and communication of information between differentkinds of actors. The studies on information management intransboundary water regimes showed that the information needsand strategies often were defined primarily with watercommissions’own needs in mind. The data collected by thecommissions were predominated by monitoring data, describingthe status of the environment and the impact caused by humanactivities. Furthermore, any communication of information toother groups of actors was mainly done through passivechannels. The information management in these transboundarywater regimes was not fully in accordance with informationneeds implied by the IWRM concept and the EU WFD.

Keywords:Integrated Water Resources Management (IWRM);transboundary water management; information; EU Water FrameworkDirective (WFD); regimes; policy and decision making; LakeNeusiedl; Lake Constance; Elbe River.

According to the Water Framework Directive of the European Union, macrophytes should be used as indicators in the ecological and environmental monitoring of lakes. In the member state Sweden the Environmental Protection Agency has elaborated assessment criteria for determining lake status based on macrophytes. The main focus of this thesis is to evaluate the efficiency of the assessment criteria for macrophytes. In addition the concordance between the ecological status classes of the four quality factors included was analysed. The assessment criteria for macrophytes are based on the total phosphorus preference of the respective species. The focus is thus mainly on the nutrient level of the investigated lakes and the environmental problem monitored is eutrophication. Macrophytes are one of the five biological quality factors used for lakes. The others are phytoplankton, diatoms, benthic fauna and fish. The two latter were included in this thesis. In addition to the biological factors the chemical factor, nutrients in lakes, i.e. the total phosphorus, was included. The macrophyte composition was investigated in two eutrophic Uppland lakes: Lake Trehörningen and Lake Edasjön. Since the assessment criteria were launched in 2007 there have been problems reported regarding the outcome of the methods. The results are sometimes inconclusive for eutrophicated lakes and especially for lakes with naturally high levels of nutrients. In this study the macrophyte species composition was compared with the nutrient level of different lakes. Some species were found in equal shares in oligotrophic lakes as in eutrophic, e.g. Nymphaea candida and Nuphar lutea. Hence they were considered less suitable as elements in the method evaluated. A couple of species were present in predominantly oligotrophic (e.g. Lobelia dortmanna) or eutrophic (e.g. Hydrocharis morsus-ranae) lakes, respectively. They were regarded as better indicators since they ”preferred” lakes either poor or rich in nutrients. It is important to point out that all the other biological quality factors used, except for macrophytes, are multimetric. I conclude that more parameters are needed for evaluation of macrophytes in lakes. Tentatively, the degree of coverage of the plants and algae, the presence and degree of coverage of invasive nonnative species, lake lowerings and the consequent composition and abundance change, all should be tested as additional parameters.  A high level of concordance was verified for the status classifications of the four quality factors in lakes of oligotrophy as well as in lakes of eutrophy, i.e. no particular  pattern based on trophy level was found. The macrophyte inventories of the two Uppland lakes both resulted in a moderate ecological status for macrophytes.

Earth observation satellites cover large areas with frequent temporal repetition and provide us with new insight into ocean and coastal processes. Ocean colour measurements from satellite remote sensing are linked to the bio-optics, which refers to the light interactions with living organisms and dissolved and suspended constituents in the aquatic environment. Human pressures have changed the aquatic ecosystems, by, for example, the increased input of nutrient and organic matter leading to eutrophication. This thesis aims to study and develop the link between bio-optical data and the remote sensing method to the monitoring and management of the Baltic Sea. The results are applied to the European Union’s Water Directives, and the Baltic Sea Action Plan from the Helsinki commission. In paper I indicators for eutrophication, chlorophyll-a concentration and Secchi depth were evaluated as a link to remote sensing observations. Chlorophyll-a measurements from an operational satellite service (paper I) were compared to conventional ship-based monitoring in paper II and showed high correlations to the in situ data. The results in paper I, II and IV show that the use of remote sensing can improve both the spatial and temporal monitoring of water quality. The number of observations increased when also using satellite data, thus facilitating the assessment of the ecological and environmental status within the European Union’s water directives. The spatial patterns make it possible to study the changes of e.g. algae blooms and terrestrial input on larger scales. Furthermore, the water quality products from satellites can offer a more holistic and easily accessible view of the information to decision makers and end-users. In paper III variable relationships between in situ bio-optical parameters, such as coloured dissolved organic matter (CDOM), dissolved organic carbon, salinity and Secchi depth, were found in different parts of the Baltic Sea. In paper IV an in situ empirical model to retrieve suspended particulate matter (SPM) from turbidity was developed and applied to remote sensing data. The use of Secchi depth as an indicator for eutrophication linked to the concentrations of chlorophyll-a and SPM and CDOM absorption was investigated in paper V. The variations in Secchi depth were affected differently by the mentioned parameters in the different regions. Therefore, one must also consider those when evaluating changes in Secchi depth and for setting target levels for water bodies. This thesis shows good examples on the benefits of incorporating bio-optical and remote sensing data to a higher extent within monitoring and management of the Baltic Sea.

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Manuscript. Paper 5: Manuscript.

Rivers and their catchments are under mounting pressure from direct channel modification, intensification of land use, and from a legacy of decades of channelisation. Recent legislation, in the form of the EU Water Framework Directive, places a greater emphasis on the management of water bodies as holistic systems, and includes the explicit consideration of hydromorphological quality, which describes the hydrologic and geomorphic elements of river habitats. These are defined specifically as hydrological regime, river continuity and river morphology. This appreciates that sediment and flow regimes, along with the channel structure, provides the 'template' on which stream ecological structure and function is built. Invertebrate fauna contribute significantly to the biodiversity of rivers, and often form the basis of monitoring river health. However much of the fundamental ecological knowledge base on the response of invertebrates to hydromorphological change needed to make informed decisions and accurate predictions, is either lacking, inadequate or contradictory. This thesis addresses some of the key potential shortcomings in recent bio-assessment that others have alluded to, but which have rarely been explored in the context of direct channel manipulations. By using two case studies of, realignment in a natural upland catchment, and flood protection engineering in an urban stream, this study investigates the sensitivity of hydromorphological impact assessment methods that rely on biodiversity patterns of benthic riverfly (Ephemeroptera, Plecoptera and Trichoptera) larva. This work employed widely used biomonitoring indices of benthic riverfly larva abundance, species richness, alpha and beta diversity, and community composition, applied over a range of spatial scales, in combination with spatially contemporaneous physical habitat data, to describe and explain community changes in response to disturbance, and patterns of natural variation. The effects of restoration were investigated using a high degree of sample replication within channels and across the wider catchment, as well as contrasting spring and autumn seasons. To assess change in a small urban channel, approaches that explicitly consider spatial elements of community data, using spatial eigenvectors analysis, were applied to spatially detrend community data and directly investigate spatial patterns. Restoration of the Rottal Burn was found to be successful in restoring habitat diversity and geomorphic processes, and in turn increasing reach scale species richness and beta diversity through the gradual arrival of rare and specialist taxa into novel habitats. Catchment scale replication revealed high variation in diversity indices of modified and undisturbed streams, and a strong temporal pattern related to antecedent flow conditions. Channels with greater habitat heterogeneity were able to maintain high gamma diversity during times of high flow stress by providing a number of low flow refuges along their length. The urban Brox Burn had surprisingly high riverfly richness and diversity driven by small scale hydraulic heterogeneity, created by bed roughness resulting in a range of microhabitats. Riverfly community responses to direct channel dredging could not be detected by measurements of average richness and diversity, however distinct changes were seen in gamma diversity, the identity of community members and their arrangement among sample patches. Impacts of sediment pollution release due to engineering were short lived and apparently had little detrimental impact on biodiversity. Strong spatial patterns of community assembly on the stream bed were uncovered, relating to longitudinal, edge and patchy patterns. Significant habitat drivers of community composition were confounded by high amounts of spatial autocorrelation, especially hydraulic variables. Due to the strongly physical and spatial nature of hydromorphological disturbance, turnover of species between sample locations at a range of scales, and the spatial arrangement of habitats and communities is of more use for detecting these types of subtle changes compared to mean richness or diversity. These findings have implications for the targeting of resources for monitoring of restoration, or engineering disturbances, in order to be sensitive to hydromorphological change. Efforts should target the main area of natural variability within the system, either replicating sampling in time or space to distinguish effects of impact. Spatial patterns, measures of beta diversity and species identity can be better exploited to identify systems with functioning geomorphological processes. Channel typologies proved misleading, and quantification of habitat and selection of control sites using multiple pre-defined criteria should be carried out. Studies of restoration operations and engineering impacts provide considerable opportunities for advancing our knowledge of the mechanisms that drive community response under a range of conditions to improve impact detection.

The Water Framework Directive (WFD) structures long-term plans for Europe's threatened water resources. Owning to the inherent and human-made complexities of the water cycle, stakeholders must move strategically to avoid crisis and restore sustainability. Yet, the reality of water resource management today is falling short on delivery. Stakeholders require strategic tools that will help them to build consensus and take action in the right direction. Using the Framework for Strategic Sustainable Development (FSSD), this study shows how Decision Support Systems can be strategically improved using a whole-systems approach grounded in basic Principles for Sustainability. In this way, stakeholders will be capable of making synchronized moves towards sustainability and thus more likely to realize the WFD’s goal of ‘good status’ for all European waterways by 2015.

En raison de leur importante capacité de réponse aux changements environnementaux, les producteurs primaires sont depuis longtemps utilisés comme indicateurs biologiques de la qualité des milieux aquatiques. La Directive Cadre Européenne sur l’Eau (2000/60/CE) préconise ainsi l’utilisation du phytoplancton, du phytobenthos et des macrophytes afin de mesurer l’état écologique des écosystèmes lacustres. Dans cette étude, ces trois compartiments ont été utilisés pour déterminer l’état écologique de cinq lacs localisés dans la région Aquitaine (Sud-Ouest de la France), lacs ayant la particularité de présenter des niveaux trophiques différents. Les résultats ont montré qu’au niveau intra-lac la composition floristique dépend fortement des variables physiques dans la plupart des systèmes tandis qu’au niveau inter-lac celle-ci dépend du niveau trophique. Le phytoplancton s’est révélé être l’indicateur de qualité le plus performant et le seul utilisable dans les eaux ouvertes, et le plus précis dans les systèmes où les macrophytes submergés étaient absents. Cependant, dans les lacs humiques, les assemblages phytoplanctoniques semblent répondre davantage à la turbidité et aux brassages fréquents de la colonne d’eau qu’aux nutriments. Dans de telles conditions, afin de mieux caractériser la qualité de l’eau, l’utilisation complémentaire du phytobenthos et des macrophytes se révèle plus pertinente. En conclusion, considérant les trois compartiments biologiques, la présente étude permet une approche complémentaire de l’état écologique des systèmes lacustres. Certaines des méthodes appliquées ici représentent un outil potentiel de détermination de la qualité de l’eau selon les exigences de la DCE, mais elles nécessitent d’être affinées pour une meilleure évaluation de l’état écologique des lacs français. Une attention particulière a été portée aux taxa exotiques observés dans ces lacs tempérés, et cet aspect doit être pris en considération lors de l’évaluation écologique. En effet, ces espèces sensiblement avantagées par les conséquences du changement climatique, peuvent altérer la structure et le fonctionnement des écosystèmes aquatiques en place, et aboutir à une perte de diversité
Due to their important ability to respond to environmental changes, primary producers have been used for a long time as biological indicators of water quality. The Water Framework Directive (2000/60/EC) recommends the use of the phytoplankton, phytobenthos (diatoms) and macrophytes in the ecological status assessment of lakes. In this study, these three compartments have been used to determine the ecological status of five lakes located in the Aquitaine region (southwest of France) with different trophic status. The results showed that within lakes, the floristic composition in most of the systems strongly depends on physical parameters whereas among lakes it depends on the trophic status. The phytoplankton proved to be the most powerful indicator of quality in open-water areas as well as in systems where submerged macrophytes are absent. However, in the humic lakes, the phytoplankton assemblages seem to respond more to turbidity and mixing than to nutrient load. Under such conditions, water quality could be underestimated and the complementary use of phytobenthos and macrophytes appears more appropriated. In conclusion, considering the three biological elements, the present study allows a complementary approach of the ecological status of the lakes. Some of the methods applied here represent a potential tool for the assessment of the ecological status in the context of WFD, but they need to be refined to assess more properly the ecological status of French lakes. A special attention was paid to exotic taxa observed in these temperate lakes, and this aspect must be taken into account in the ecological assessment. Indeed, these species favoured by the consequences of the climate change, can modify the structure and functioning of the aquatic ecosystems, and lead to a loss of diversity

Neste estudo desenvolve-se uma análise sobre a relação entre o contexto familiar e o profissional, mais concretamente sobre o Equilíbrio Trabalho- Família (ETF) e o Conflito Trabalho-Família (CTF). Foi estruturado um modelo de antecedentes em que se prevê que o ETF pode ser potenciado pela existência de Políticas Organizacionais de Conciliação (POC) e pela percepção de determinadas competências individuais, como a Gestão do Tempo (GT) e que o CTF pode ser predito pela realização de horas extra e pela percepção ineficaz de GT. Através da aplicação de um questionário a 322 participantes, medindo estas variáveis, constatou-se que a existência de algumas POC (parentalidade, saúde e apoio social e suporte organizacional e das chefias) e a percepção de GT predizem o ETF, enquanto a realização de horas extra prediz o CTF. São discutidos os resultados encontrados de acordo com as contribuições para o desenvolvimento teórico e as potenciais implicações para a prática profissional; ### ABSTRACT: This study develops an analysis of the relationship between family and professional background, more concretely on the Work-Family Balance (WFB) and the Work-Family Conflict (WFC). We built a model of precedents which predicts that the WFB can be enhanced by the existence of Organizational Policies for Reconciling Work-Family Life (OPR) and the perception of certain individual skills such as Time Management (TM), and that the WFC can be predicted by the performance of overtime and the perception of ineffective TM. We administered a questionnaire to 322 participants, measuring these variables; we found that the existence of some OPR (parenting, health and social support and organizational support and leadership) and the perception of TM predict the WFB, whilst working overtime predicts the extra WFC. The results are discussed according to the contributions to theoretical development and the potential implications for professional practice.

Même si la réduction des apports de nutriments dans les écosystèmes aquatiques est généralement envisagée avec des approches « effects-based », ce n’est pas le cadre qui a été suivi dans cette thèse. En effet, dans une démarche de développement durable, il semble plus pertinent, de définir des mesures de contrôle de la pollution agissant sur les relations de cause à effet. Selon cette logique, la méthodologie proposée par la Directive Cadre sur l’Eau (DCE) pour protéger les écosystèmes aquatiques présente plusieurs faiblesses. Par le fait qu’elle réfère à certains principes de l’économie standard de l’environnement, l’analyse économique de la DCE pose plusieurs problèmes pour agir sur la causalité de la pollution. Néanmoins, la méthodologie proposée par la DCE est pertinente par le fait qu’elle recommande l’utilisation combinée de mesures techniques et d’instruments pour diminuer les dommages environnementaux. Conformément à cette disposition, l’effet de mesures techniques et d’instruments sur le secteur agricole et sur la chaine alimentaire l’englobant a été évalué puisque cette dernière est responsable de la majorité des émissions diffuses et ponctuelles d’azote et de phosphore dans les eaux de surface. La définition de mesures techniques en fonction de l’efficience de l’utilisation des nutriments (Nutrient Use Efficiency, NUE) dans la production agricole a été complétée par l’étude des instruments économiques utilisés dans la Politique Agricole Commune (PAC) pour développer une agriculture multifonctionnelle. Si la thèse concerne de manière générale l’eutrophisation des écosystèmes aquatiques, elle se penche plus particulièrement sur les pressions au sein du bassin de l’Escaut et sur leurs impacts jusqu’à la zone côtière de la mer du Nord. Des mesures techniques agissant sur les causes des émissions diffuses du secteur agricole et sur les émissions ponctuelles des stations d’épuration ont été proposées pour ce bassin. Ces mesures techniques ont été caractérisées prioritairement par leur efficacité environnementale (grâce aux modèles Sénèque-Riverstrahler et MIRO) et en fonction de leurs coûts directs.

/

Although reducing the load of nutrients in aquatic ecosystems is usually looked at through "effects-based" approaches, this is not the context which this paper has chosen to follow. In fact, keeping in line with sustainable development, it seems more relevant to define measures to control pollution which act on cause and effect relations. According to this way of thinking, the methodology proposed by the Water Framework Directive (WFD) to protect aquatic ecosystems presents a number of weaknesses. By the fact that it refers to certain standard economic principles for the environment, the WFD’s economic analysis poses several problems towards acting on the cause of the pollution. Nevertheless, the methodology proposed by the WFD is relevant, as it recommends the combined use of technical measures and of instruments to reduce environmental damage. In accordance with this disposition, an assessment has been made of the effect of technical measures and instruments on the agricultural sector and on the food chain encompassing it, since the food chain is responsible for the majority of diffuse and point emissions of nitrogen and phosphorus in surface waters. The definition of technical measures in terms of efficient use of nutrients (Nutrient Use Efficiency, NUE) in agricultural production has been complemented by a study of economic instruments used in the Common Agricultural Politicy (CAP) to develop a multifunctional type of agriculture. Although the paper deals with the eutrophication of aquatic ecosystems in a general way, it concentrates particularly on the increasing pressures in the Scheldt basin and on their impacts as far as the North Sea coastal zone. Technical measures acting on the causes of diffuse emissions in the agricultural sector and on point emissions in the water treatment plants have been proposed for this basin. These technical measures have been categorised in order of priority according to their environmental effectiveness (thanks to the Sénèque-Riverstrahler et MIRO models) and depending on their direct costs.
Doctorat en Sciences agronomiques et ingénierie biologique
info:eu-repo/semantics/nonPublished

The Water Framework Directive (Directive 2000/60/EC) is an innovative piece of legislation aimed at harmonizing Water Policy among the 27 Member States. This Directive, the reading of which may appear quasi-revolutionary due to its territorial and material scope, is a new policy instrument with, as of yet, still ongoing implementation. The final deadline for the complete implementation of the Water Framework Directive is 2015, when the good ecological status of the water bodies in the European Union should have been achieved. The purpose of this study is to analyze the development of the implementation on three essential aspects of the Directive: the transposition, the creation of the administrative authorities, and the public participation in the elaboration of the River Basin Management Plans. My thesis has two main research questions: 1) Have France and Spain implemented the WFD correctly? 2) Does the pervasive theory of the Mediterranean Syndrome apply to my study cases? In order to answer the first question, I use the scoreboard method to assess of the correctness of implementation of the three essential aspects of the Directive mentioned above, complemented by an examination of the domestic dynamics that shaped the implementation categorized into different modes of governance. To address the second question the two case studies I have chosen will test the doctrine of the northern leaders and southern laggards as based on institutional culture. The doctrine of the Southern Laggards (also known as the Mediterranean Syndrome doctrine) advocates that the Southern/Mediterranean states are doomed to fail implementing EU environmental policy. In my thesis, I address this doctrine by using a practical case study: the comparison of the implementation of the Water Framework Directive between France and Spain. The essence of these arguments is rooted in the mainstream doctrine of the goodness of fit. That is, a Directive is more likely to be correctly implemented where there is a matching institutional framework. In order to use this theory as a departure point to examine the hypothesis of likeliness of correct implementation of the WFD by France and Spain, I selected three main institutional features that are hallmarks in France and Spain and also practically opposed: political centralization versus decentralization, democracy and participation versus totalitarianism, and the status of economic development of each EU Member. I use them as indicators to determine the degree of fit of France and Spain´s institutional frameworks with the purpose, the policy style and the institutions required by the WFD. I conclude with highlighting the importance of this study for the contribution that it may make to two contemporary issues: the current Eastern/Western divide in the EU (can the doctrine of the Northern/Southern states usefully be extrapolated to the Western/Eastern Members?) and the thorny economic situation of the EU today. At the time of finishing writing this thesis, all the fingers point to Greece, immersed in a striking public debt that has shaken the rest of the Mediterranean countries: is the Mediterranean Syndrome attacking again?
Graduate

The problematic of Contaminants of Emerging Concern (CEC) in the European Union (EU) surface water has been reported over the last decades as well as CEC’s potential impact on human health and the environment. The Water Framework Directive (2000/60/EC; WFD) is the main EU Directive for the protection of aquatic environments since it came in force. This Directive requires the monitoring of 45 priority substances regarding their environmental quality standards in order to achieve the good chemical status of water bodies until 2027. The last revision of the WFD in 2015 showed that Member States have not met the targets yet. The present dissertation aims to analyse whether WFD has been efficient enough to protect the aquatic ecosystems against CEC and evaluate if the environmental objectives will be complied until its last revision. The Netherlands was used as the case study. Interviews were carried out to 13 employees of the different layers in the Dutch water management system. Based on results obtained, it was concluded that the WFD has been the main European water legislation used to protect the aquatic environment from the occurrence of CEC in the last years. The Directive have created awareness and encourages Member States to take actions, however, some issues were identified. The conclusion is that WFD has not been efficient enough in the protection of aquatic environments against CEC. Although it is difficult to predict its success in 2027, two possible scenarios were identified. In the end, it was suggested a consistent assessment of CEC in aquatic environments, connection of WFD goals with EU’s chemicals regulations, collaboration between all interested parties and an integrated strategy to WFD implementation in the Member States.

The thesis is aimed on hydromorphological assessment with usage of distance data. The distance method QuaWaDis is defined and the usage of results of image processing within the hydromorphological assessment is considered. The aim of the assessment is classification of hydromorphological conditions of water bodies according to Water Framework Directive 2000/60/EC. Distance method is based on visual interpretation of public data. Image processing results can extend the number of parameters of the distance method - object based classification was applied on aerial imagery (in land cover classification), unsupervised classification and image reclassification was applied on multispectral satellite data (in water depths classification). The calibration of distance method and the credibility of the distance data was evaluated in comparison with results of field survey EcoRivHab (Matoušková, 2008). Object based image processing was aimed on accuracy assessment, index of efficiency was defined and the concept of fuzzy logic was applied in accuracy assessment. From consideration of classification efficiency is obvious, that large internally heterogeneous objecs is more effectivelly to vectorise manually. Accuracy assessment with usage of concept of fuzzy logic shows the success of object recognition within each...

Pesticides are vast class of compounds formulated to control pest. They are applied all over the world and their presence in the environment, especially in coastal lagoons has been inevitable. The knowledge gap concerning pesticides fate in coastal lagoons and their impact on the aquatic organisms as well as the lack of sensible analytical methods for their measurement was the driving force of this work. Focus was made on the pesticides classified as priority substances within the Water Framework Directive (WFD). Óbidos Lagoon (Leiria, Portugal) is of economic importance and as any coastal lagoon, is exposed to anthropogenic activities being for those reason the selected area for all the studies. To fulfill the analytical gaps concerning the priority pesticides (PPs), sensible analytical methodologies were developed for the determination of those pesticides in sediments and macroalgae Ulva sp. of Óbidos Lagoon. The application of such methodologies allowed an in-depth knowledge of pesticides historical application, sources and pathways inside the lagoon. Interestingly, results in the analysis of Ulva sp. show a tendency to accumulate some of the PPs only under adverse weather conditions which points the importance of the climate global changes in the uptake and partition of the PPs in coastal lagoons. Rural activities in the watershed were found to be the main source of the PPs in Óbidos Lagoon. Soil runoff and discharges through small tributaries are the main vehicles of entrance into the lagoon. PPs sediments monitoring reveals that past and present applications of those compounds have been carried out. The low hydrodynamism of the lagoon branches (Barrosa) favors the retention of the pesticides in this part of the lagoon. Among the list of the studied PPs, lindane, p,p’-DDT and the metabolite heptachlor epoxide show to be at levels above the ‘’probable level effect’’ with possible adverse impacts in aquatic organisms. Dissipation studies with chlorpyrifos revealed the importance of salinity, water turbulence and addition of dissolved organic matter (DOM) in its environmental fate. DOM is a very complex mixture of thousands of organic compounds with different sizes, charges and polarity. The development of a new gradient elution extraction methodology that uses a mixture of eluents with different polarities allowed the fractionation of DOM based in its hydrophobicity. Improving knowledge of DOM molecular-level composition is crucial for a better understanding of its reactivity and consequently its impact on pesticides environmental fate.

This thesis provides an analysis how social learning can be supported during the implementation of the WFD. Four research questions address the different characteristics of river basin management: 1) How do spatial misfits between participatory and decision-making institutions impede social learning"2) Does the formalized context of river basin management in Europe allow for social learning" 3) What are conducive environments for social learning in river basin management" 4) How does research contribute to social learning in river basin management" What challenges arise in applied research"The concept of social learning (SL), especially as developed in the European project HarmoniCOP (www.harmonicop.uos.de), formed the theoretical backbone of the analysis. This allowed to consider the challenge of SL to be supported by process design or the institutional settings. At the same time, it allowed to understand to which extent the efficiency of this support depends on the question whether or not the participants in the process recognize the options for improving their knowledge base and make use of them. The analyses have been mainly based on a German case study on the international Elbe river basin district, which was embedded in the European project HarmoniCOP. Here, the specific challenges (spatial misfit; the need for collaboration in formalized contexts) have been addressed exploring participatory and other collaboration processes as well as the actors approach to SL. In order to understand collaboration processes between research and water management, the role of research in delivering tools to support the new challenges of the WFD has been studied during activities of the European Concerted Action Harmoni-CA (www.harmoni-ca.info). The thesis closes with recommendations to improve social learning in practical river basin management.

Τα παράκτια μεταβατικά οικοσυστήματα όπως οι λιμνοθάλασσες και οι αλυκές, αποτελούν δυναμικά οικοσυστήματα και παρουσιάζουν έντονες χωρικές και χρονικές διακυμάνσεις, αφού ως οικότονοι μεταξύ ξηράς και θάλασσας, δέχονται την ταυτόχρονη επίδραση χερσαίου και θαλάσσιου περιβάλλοντος. Παράλληλα είναι οικοσυστήματα υψηλής παραγωγικότητας, αφού φιλοξενούν είδη με υψηλή πρωτογενή παραγωγικότητα. Τα υδρόβια μακρόφυτα αποτελούν δομικά και λειτουργικά στοιχεία των οικοσυστημάτων αυτών και σύμφωνα με την Οδηγία Πλαίσιο 2000/60/ΕΕ, αποτελούν ποιοτικά στοιχεία και χρησιμοποιούνται ως βιοδείκτες για την εκτίμηση της οικολογικής ποιότητας των υδάτινων οικοσυστημάτων. Στα πλαίσια της παρούσας μεταπτυχιακής διατριβής, διερευνήθηκαν οι σχέσεις μεταξύ αβιοτικών και βιοτικών παραμέτρων της υδάτινης στήλης, στις λιμνοθάλασσες Κοτύχι και Πρόκοπο της ΒΔ Πελοποννήσου και στις αλυκές Λάρνακας και στον υγρότοπο Ακρωτηρίου (Αλυκές Ακρωτηρίου, λιβάδι Φασουρίου) της Κύπρου. Πραγματοποιήθηκαν μηνιαίες δειγματοληψίες κατά την βλαστητική περίοδο των ετών 2006, 2007 και 2008, στην διάρκεια των οποίων καταγράφηκαν οι φυσικοχημικές παράμετροι των υδάτων, όπως βάθος (m), διαφάνεια (m), θερμοκρασία (οC), αλατότητα (‰), αγωγιμότητα (mS/cm), διαλυμένο οξυγόνο (mg/l), pH, ολικά αιωρούμενα στερεά (mg/l), φωτοσυνθετικά ενεργή ακτινοβολία- PAR ενώ υπολογίστηκαν οι συγκεντρώσεις της Chl-α (mg/m3) η αλκαλικότητα (mg/l), η συγκέντρωση των θρεπτικών αλάτων φωσφόρου-SRP (mg/l) και αζώτου - NO2-N, NO3-N και ΝΗ4-Ν (mg/l), καθώς και η σταθερά απορρόφησης Κ (m-1) της υδάτινης στήλης. Παράλληλα συλλέχθηκαν ποσοτικά και ποιοτικά δεδομένα που αφορούν τη δομή των μακροφυτικών κοινωνιών, καθώς και την χωρική και χρονική διακύμανση της κατανομής και της ανάπτυξης των μακροφυτικών ειδών που απαντώνται στις λιμνοθάλασσες και στις αλυκές. Τέλος διερευνήθηκαν οι σχέσεις μεταξύ αβιοτικών παραμέτρων και ειδών μακροφύτων και εξετάστηκαν οι πιθανοί παράγοντες που επηρεάζουν την ανάπτυξη και την κατανομή των μακροφύτων. Για την διερεύνηση της δομής των μακροφυτικών κοινωνιών, την ταξινόμηση της υδρόβιας βλάστησης σε ευδιάκριτες ομάδες, καθώς και την ομαδοποίηση των σταθμών δειγματοληψίας ανάλογα με την σύνθεση της βλάστησης τους, εφαρμόστηκαν οι μέθοδοι ταξινόμησης TWINSPAN και MDS. Χρησιμοποιήθηκαν επίσης οι συντελεστές συσχέτισης Pearson (r) και Kendall (tau-b), προκειμένου να εξεταστεί η ύπαρξη πιθανών συσχετίσεων μεταξύ των φυσικοχημικών παραμέτρων, αλλά και μεταξύ βιοτικών και αβιοτικών παραμέτρων, ενώ πραγματοποιήθηκε ανάλυση διασποράς (one-way ANOVA) προκειμένου να διαπιστωθούν στατιστικά σημαντικές διαφορές μεταξύ των περιοχών μελέτης, αλλά και μεταξύ των διαφορετικών ομάδων βλάστησης. Τέλος, για την διερεύνηση των αβιοτικών παραμέτρων που επηρεάζουν την ανάπτυξη και την κατανομή των μακροφύτων, εφαρμόστηκε η μέθοδος ανάλυσης κανονικών αντιστοιχιών CCA (Canonical Correspondence Analysis). Στις τέσσερις περιοχές μελέτης καταγράφηκαν συνολικά 29 taxa μακροφύτων, τα οποία ταξινομήθηκαν σε έξι ομάδες βλάστησης. Πέντε taxa από αυτά, αναφέρονται για πρώτη φορά στην Κύπρο. Τα είδη με την μεγαλύτερη αφθονία στις περιοχές μελέτης ήταν τα Ruppia cirrhosa, Potamogeton pectinatus, Najas marina ssp. armata, Althenia filiformis, Lamprothamnium papulosum και Ulva lactuca. Η βιομάζα των κυρίαρχων ειδών παρουσίασε εποχικές διακυμάνσεις με τις μέγιστες τιμές να καταγράφονται την άνοιξη και το καλοκαίρι. Στις λιμνοθάλασσες Κοτύχι και Πρόκοπο παρατηρήθηκε οικολογική διαδοχή ειδών στη διάρκεια της βλαστητικής περιόδου, η οποία μπορεί να αποδοθεί σε σημαντικές αλλαγές του αβιοτικού περιβάλλοντος (π.χ. αλατότητας), όπως η αντικατάσταση του είδους Potamogeton pectinatus από το αγγειόσπερμο Ruppia cirrhosa, ενώ η εμφάνιση ευτροφικών φαινομένων με σημαντική αύξηση της βιομάζας ευκαιριακών ειδών (π.χ. χλωρόφυτα), οδήγησε στην μείωση της βιομάζας των αγγειοσπέρμων. Στις αλυκές Λάρνακας και Ακρωτηρίου η βιομάζα κυμάνθηκε σε πολύ χαμηλά επίπεδα πιθανώς λόγω των πολύ ψηλών τιμών αλατότητας. Τις υψηλότερες τιμές βιομάζας παρουσίασε το αγγειόσπερμο Althenia filiformis τον Απρίλιο. Την βλαστητική περίοδο 2008 οι μεγαλύτερες τιμές αλατότητας που καταγράφηκαν στις αλυκές Λάρνακας και Ακρωτηρίου, δεν ευνόησαν την ανάπτυξη μακροφύτων. Αντίθετα στο λιβάδι Φασουρίου όπου η αλατότητα ήταν σημαντικά χαμηλότερη, η βιομάζα του κυρίαρχου Najas marina ssp. armata κυμάνθηκε σε υψηλές τιμές με τις μεγαλύτερες να καταγράφονται τον Ιούνιο. Από την διερεύνηση των μορφομετρικών χαρακτηριστικών του αγγειόσπερμου Ruppia cirrhosa, προέκυψε ότι η πυκνότητα των πληθυσμών του είδους κυμάνθηκε στα ίδια επίπεδα με άλλες λιμνοθάλασσες της Μεσογείου (449–5120 βλαστοί/m2), ενώ παρουσίασε θετική συσχέτιση με την θερμοκρασία και αρνητική συσχέτιση με την συγκέντρωση ολικού φωσφόρου. Το μήκος των φύλλων συσχετίστηκε θετικά με την συγκέντρωση των όξινων ανθρακικών ιόντων, τα οποία σε χαμηλές συγκεντρώσεις μπορεί να αποτελέσουν περιοριστικό παράγοντα για την ανάπτυξη των μακροφύτων. Η μεγαλύτερη ποικιλότητα μακροφύτων (δείκτης Shannon-Weaner) καταγράφηκε στην λιμνοθάλασσα Πρόκοπο, ενώ οι αλυκές Λάρνακας παρουσίασαν την χαμηλότερη ποικιλότητα, πιθανά λόγω του ότι οι υψηλές τιμές αλατότητας δεν ευνοούν την ανάπτυξη πολλών ειδών μακροφύτων. Η εφαρμογή του δείκτη οικολογικής αξιολόγησης ΕΕΙ (Ecological Evaluation Index) για την εκτίμηση της οικολογικής ποιότητας των υδάτων με την χρήση των μακροφύτων ως βιοδεικτών, είχε ως αποτέλεσμα την ταξινόμηση της ποιότητας των υδάτων της λιμνοθάλασσας Κοτυχίου ως Μέτρια, της λιμνοθάλασσας Πρόκοπος και των αλυκών Λάρνακας ως Καλή, ενώ του υγρότοπου Ακρωτηρίου μπορούν να χαρακτηριστούν ως Υψηλής οικολογικής ποιότητας. Oι σημαντικότερες περιβαλλοντικές παράμετροι που καθορίζουν την σύνθεση των ομάδων βλάστησης, αλλά και την εξάπλωση των ειδών των μακροφύτων στις περιοχές έρευνας, είναι το υδατικό ισοζύγιο, η αλατότητα και οι συγκεντρώσεις των θρεπτικών αλάτων. Στα πλαίσια προστασίας και διατήρησης των παράκτιων αυτών υγροτόπων, προτείνεται ο σχεδιασμός ενός μόνιμου δικτύου παρακολούθησης βιοτικών και αβιοτικών παραμέτρων και η διεξαγωγή συστηματικής έρευνας με την πραγματοποίηση οικολογικών μελετών χρησιμοποιώντας βιοτικά στοιχεία για την εκτίμηση της οικολογικής τους ποιότητας. Επίσης, η λήψη διαχειριστικών μέτρων για τον περιορισμό της εισροής θρεπτικών στους υγρότοπους, κρίνεται επιτακτική αφού η εμφάνιση ευτροφικών φαινομένων αποτελεί διαρκεί απειλή για τα υδάτινα οικοσυστήματα. Η απαγόρευση καταπάτησης των οικοτόπων, της απόρριψης σκουπιδιών, της βόσκησης και της θήρευσης, μπορεί να υλοποιηθεί με την λήψη και εφαρμογή αυστηρών μέτρων, καθώς και με την φύλαξη των περιοχών αυτών. Η περίφραξη και η σηματοδότηση επιλεγμένων σημείων, ο καθαρισμός απορριμμάτων, η απομάκρυνση ξενικών ειδών και ο καθαρισμός των καναλιών που συλλέγουν και διοχετεύουν νερό από την λεκάνη απορροής στους υγρότοπους, μπορούν να βοηθήσουν στην διατήρηση των υγροτόπων και την βελτίωση της οικολογικής τους κατάστασης. Επίσης, η πραγματοποίηση ειδικών περιβαλλοντικών μελετών πρέπει πάντα να προηγείται της υλοποίησης κάθε μορφής έργων. Τέλος η ενημέρωση και ευαισθητοποίηση του κοινού με την απρόσκοπτη λειτουργία κέντρων περιβαλλοντικής ενημέρωσης και φορέων διαχείρισης των περιοχών αυτών, κρίνεται απαραίτητη προκειμένου οι υγρότοποι να προστατευτούν και να αποφευχθεί η σταδιακή υποβάθμιση τους.
Lagoons and other coastal wetlands are shallow aquatic environments located in the transitional zone between terrestrial and marine ecosystems, which can span from freshwater to hypersaline conditions depending on their water balance. They exhibit an extreme spatial and temporal variability of environmental parameters and are recognized as highly productive ecosystems. Aquatic macrophytes are key structural and functional components of aquatic ecosystems. As photosynthetic sessile organisms being at the base of food web, are vulnerable and adaptive to human and environmental stress. They respond to aquatic environment representing reliable indicators of its changes and are mentioned in the WFD as biological quality elements for the ecological classification of transitional and coastal waters. In the present work, the relationships between biotic and abiotic parameters of the water column were investigated in coastal lagoons of Kotychi and Prokopos (NW Peloponnisos, Greece), Larnaca salt lakes and Akrotiri wetland (Cyprus). Monthly samplings were conducted during the vegetative periods of the years 2006, 2007 and 2008 and water parameters such as depth (m), transparency (m), temperature (οC), salinity (‰), conductivity (mS/cm), dissolved oxygen (mg/l), pH, photosynthetic active radiation – PAR and attenuation coefficient K (m-1) were recorded in situ, while Chl-α (mg/m3), total suspended matter (mg/l), alkalinity and nutrients of nitrogen and phosphorus (mg/l) were determined in the laboratory. Quantitative and qualitative data concerning aquatic macrophyte flora were recorded during the same period, such as species composition, community structure, spatial and temporal variations of species abundance and the succession of submerged macrophytes in relation to the main environmental factors. The aquatic macrophytic community was distinguished in different vegetation groups using TWINSPAN and MDS techniques. Correlations between environmental parameters were tested using Pearson and Kendall correlation tests, while One –way ANOVA was used to determine statistical significant differences of environmental variables between different study areas and distinguished plant groups. Finally CCA was performed in order to examine relationships between the species abundance and environmental variables. In total, 29 taxa were recorded in all four study areas and were classified in six vegetation groups. Five species were recorded for the first time in the island of Cyprus. The most abundant species were Ruppia cirrhosa, Potamogeton pectinatus, Najas marina ssp. armata, Althenia filiformis, Lamprothamnium papulosum και Ulva lactuca. The biomass of dominant species showed seasonal variations and maximum biomass values were recorded in late spring and summer. In Kotychi and Prokopos lagoons, the replacement of Potamogeton pectinatus by Ruppia cirrhosa was recorded, probably due to significant increase of salinity. In other parts of the lagoons, the replacement of macrophyte beds due to increased macroalgal proliferation was also recorded. In Larnaca and Akrotiri salt lakes, biomass values were significantly lower as a result of high salinity values. The most abundant species was Althenia filiformis (biomass peak in April). During the vegetative period of 2008, extreme salinity values were recorded as a result of reduced rainfall. High salinity values resulted in scarse occurrence of macrophytes in the salt lakes during this period. In Fasouri marsh, which is characterized by minimum salinity values, the dominant species Najas marina ssp. armata showed high biomass values (peak in June). Regarding the results of Ruppia cirrhosa population’s density in Kotychi lagoon (449-5120 shoots/m2), they were comparable with data from other Mediterranean lagoons. Meadows density was positively correlated with temperature and negatively correlated with total phosphorus. Leaf length showed positive correlation with bicarbonate ions, which can be a restrictive factor for the growth of macrophytes. The highest macrophyte diversity (Shannon-Weaner index) was found in Prokopos lagoon, whereas Larnaca salt lakes had the lowest values, probably due to high salinity. The implementation of EEI (Ecological Evaluation Index) for the assessment of the ecological quality of coastal and transitional waters, showed that Kotychi lagoon was classified into Moderate ecological class. Prokopos lagoon and Larnaca salt lakes were classified into Good ecological class and finally Akrotiri wetland was classified into High ecological class. Hydrological regime, salinity and nutrient concentrations seem to be the key factors controlling macrophyte composition, community structure and species abundance. For the conservation and management of these coastal and transitional wetlands, we propose the development of a monitoring network for biotic and abiotic parameters. Ecological studies for the evaluation of ecological quality of the wetlands according to the WFD 200/60/EU and implementation of management practices for the reduction of nutrient inflows in the wetlands are needed, in order to reduce eutrophication phenomena, which are a severe threat for the ecological balance of the wetlands. The encroachment of wetlands, hunting and waste discharges, should be prohibited by law. Finally, the increase of public environmental awareness will have significant results in the conservation of coastal wetlands.

The cumulative dissertation at hand addresses the question of how promising the successful implementation of the WFD is. Since research on implementation deficits has so far mainly considered national and sub-national levels, regional and local scales are taken centre stage within the dissertation. The Hase river catchment, which is located in one of Germany s most intensive livestock areas, serves as an example. The thesis shows that the procedural character of the WFD and regulative uncertainties give way to interpretation and action leeway, which will be pivotal to judgement on implementation success at lower levels. Moreover, the consistent operationalisation of nitrate limits in particular has led to major difficulties during implementation in Germany from which restraints can be expected for the preparation of river basin management plans and the program of measures. As to the regional level, implementation restraints also upraise due to socio-economic dependencies from agriculture and a high identification of the regional population with this economic sector have placed agriculture in a powerful position. In conclusion, one can state that various implementation restraints occur and the cause of these can be found at all scales European, sub-national and regional. The results of this dissertation concerning the multi-level system reveal that the relationship and interaction of the various scales will be given increased attention during the implementation process of European environmental policy. Besides the national and sub-national scale, the regional and local levels will be of equal importance. The question of suitable scales for implementation decisions and measures also offers pivotal approaches to undertake future research. Further research needs occur with new regional studies at national and international scales, with regard to the future of water protection in the course of the reform of German federalism and the current debate on climate change.