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Academic literature on the topic 'Wb 60'

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Published: 25 July 2024
Last updated: 29 July 2024

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Journal articles on the topic "Wb 60"

1

Kaiser, Martin F., Nuria Porta, Bhupinder Sharma, Daniel Levine, Dow-Mu Koh, Kevin Boyd, Charlotte Pawlyn, et al. "Prospective comparison of whole body MRI and FDG PET/CT for detection of multiple myeloma and correlation with markers of disease burden: Results of the iTIMM trial." Journal of Clinical Oncology 39, no. 15_suppl (May 20, 2021): 8012. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.8012.

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8012 Background: Early and sensitive detection of bone marrow disease and stratified patient management according to clinical risk can confer survival advantages in multiple myeloma (MM). Whole body MRI (WB MRI) and Fluorodeoxyglucose (FDG) PET/CT are included in international guidelines for imaging in patients with a suspected diagnosis of MM. However prospective studies comparing detection of MM by contemporary WB MRI as per recent MY-RADS consensus against FDG PET/CT are lacking. We report here protocol-defined endpoints from the prospective iTIMM (NCT02403102) study, comparing WB MRI and PET/CT, their relationship with serum and bone marrow estimates of disease burden, as well as molecular tumor characteristics. Methods: Patients with newly diagnosed MM or at first relapse planned to receive chemotherapy and autologous stem cell transplantation were enrolled in iTIMM. Matched baseline WB MRI and FDG PET/CT were performed and baseline clinical data including tumor genetics collected. Scans were double reported for presence of focal and diffuse disease by expert MRI and PET/CT radiologists, blinded to each other’s assessment. Paired methods were used to compare burden and patterns of disease on WB MRI compared to FDG PET/CT at baseline. Primary and secondary trial endpoints include relationship between post-treatment WB MRI response and progression-free survival, for which follow-up is ongoing. Exploratory endpoints include comparison of baseline WB MRI and PET/CT and their correlation with laboratory parameters, for which data is complete and reported here. Results: From May 2015 to March 2018, sixty patients (35 male; mean age 60 years) underwent baseline WB MRI as per MY-RADS consensus and FDG PET/CT. At least one focal lesion was detected in 50/60 patients (83.3%) by WB MRI and in 36/60 patients (60%) by PET/CT. WB MRI was more sensitive ( P< 0.05) across anatomical regions except for ribs and cervical spine. Four patients in our study showed two or more focal lesions ≥5 mm only on WB MRI but not PET/CT. All lesions detected by WB MRI but not PET/CT resolved in follow-up scans after treatment, excluding false positives. In 49/60 (81.7%) patients, diffuse disease was detected by WB MRI, compared to 10/60 (16.7%) by PET-CT; WB MRI was more sensitive across all anatomical areas ( P< 0.05). Plasma cell infiltration and paraprotein levels were significantly higher for patients with diffuse disease on WB MRI, but not on PET/CT. All genetically high-risk tumours, defined by t(4;14), t(14;16), del(1p), gain(1q) or del(17p), showed diffuse infiltration on WB MRI. Conclusions: WB MRI increases detection of focal and diffuse disease compared with FDG PET/CT, including improved detection of focal lesions meeting criteria for active disease as per International Myeloma Working Group diagnostic criteria, proposing it as a gold standard for tumor imaging in MM. Clinical trial information: NCT02403102.
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2

Chen, Ying, Duanwei He, Jiaqian Qin, Zili Kou, Shanmin Wang, and Jianghua Wang. "Ultrahigh-pressure densification of nanocrystalline WB ceramics." Journal of Materials Research 25, no. 4 (April 2010): 637–40. http://dx.doi.org/10.1557/jmr.2010.0082.

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Phase-pure nanostructured WB ceramics are hot pressed at ultrahigh pressures of 1.0 to 3.0 GPa and high temperatures of 700 to 1000 °C (UHPHT) for 60 min. The UHPHT samples are nanograin size from 15 to 40 nm. Our experimental observation shows that ultrahigh pressure could improve densification, and the density of WB samples could reach 99.4% of theoretical. The comparative experiments carried out at ambient pressure and temperatures of 550 to 1100 °C for 60 min indicate that the external pressure was favorable for phase-pure and highly dense WB formation. In addition, the UHPHT samples give a high hardness value of 28.9 ± 0.8 GPa.
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Thodis, Panagiotis, Ioanna S. Kosma, Konstantinos Nesseris, Anastasia V. Badeka, and Michael G. Kontominas. "Evaluation of a New Bulk Packaging Container for the Ripening of Feta Cheese." Foods 12, no. 11 (May 28, 2023): 2176. http://dx.doi.org/10.3390/foods12112176.

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In the present study, the quality characteristics of Feta cheese were investigated as a function of the packaging container (a stainless-steel tank (SST), a wooden barrel (WB), and a tin can (TC)) and ripening time. The results showed that the Feta cheese’s pH, moisture, and lactose decreased, while fat, protein, and salt increased (p < 0.05) during ripening with SST and WB, showing similar behaviors versus that of the TC container. For the proteolysis indices, % TN,% WSN, 12% TCA, and 5% PTA showed the highest values (p < 0.05) for cheeses packaged in WB, followed by those in SST and TC, with all increasing (p < 0.05) during ripening. The most abundant odor-active volatiles were free fatty acids, alcohols, and esters following the order SST > WB > TC on day 60. On day 60, the cheeses packaged in SST and WB showed higher (p < 0.05) hardness and fracturability values, as well as aroma scores, compared to those in TC, with both parameter values increasing with the ripening time.
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Le Bris, Yannick, Berger Florian, Audrey Menard, Thierry Guillaume, Pierre Peterlin, Alice Garnier, Laurence Lode, et al. "Engraftment of Donor Cells after Allogeneic Stem Cell Transplantation: Comparison and Impact of Chimerism in Whole Blood and Peripheral CD3+ T-Cells." Blood 128, no. 22 (December 2, 2016): 5866. http://dx.doi.org/10.1182/blood.v128.22.5866.5866.

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Abstract Introduction: After allogeneic stem cell transplantation (allo-SCT), engraftment can be assessed by quantitative polymerase chain reaction (qPCR) using differing donor/recipient markers (Alizadeh et al. Blood, 2002), identified in peripheral blood DNA cells before transplant. We report here on the concomitant examination of the proportions of donor and recipient DNA in peripheral whole blood (WB) and sorted CD3+ T-cells on days +60 and +90, looking at their impact on survival. Patients, material and methods: This monocentric study evaluated the impact on outcomes of early WB and sorted CD3+ T cells chimerism independently and of the four possible combinations of chimerism between WB and sorted CD3+ T-cells. All follow-up chimerism samples from allo-SCT patients performed in adults at Nantes University Hospital between October 2009 and October 2016 were reviewed, focusing on those where both PB and/or CD3+ T-cells were evaluated on days +60 (45-75) and/or +90 (75-120) after allo-SCT. A global cohort of 229 patients (239 grafts) was retrieved, which includes 52 patients evaluable on day +60 only, 67 evaluable on day +90 only and 120 evaluable on both days +60 and +90. A threshold of >95% donor DNA was considered for complete chimerism. Disease free survival (DFS) was calculated from the date of graft until relapse, death or last follow-up. Overall survival (OS) was calculated from the date of graft until death or last follow-up. Chi square tests were used to compare incidences. Log rang test and Kaplan Meier were used to evaluate DFS and OS. Results: The whole cohort comprised 62% males and had a median age of 58 years old (20-74) at the time of allo-SCT. Patients were treated for myeloid-lineage disease in 59% of the cases. Reduced-intensity conditioning was used in 89% (n=212), donors were familial in 45% (n=107), registry in 48% (n=114). Unrelated cord blood units were used in 8% of the cases (n=18). Post-transplant cyclophosphamide (PTCY) was performed in 48 procedures including 33 and 15 with haplo (HG) and matched donors respectively. Considering the 239 allograft procedures, the median follow-up was 5.8 years (95% CI: 3.1-5.8), the rate of relapse 27% and the rate of death 31%. Complete WB chimerism was observed for 80% and 71% of the cases on day +60 and day +90 respectively. Complete CD3+ chimerism was present for 53% and 51% of the grafts at days +30 and +90 respectively. Thus, cases displaying both complete WB and CD3+ chimerism on days +60 and +90 were 53% and 51% respectively, while 27% and 20% were documented with full WB and mixed CD3+ chimerism on days +60 and +90. Mixed chimerism was observed in both WB and CD3+ cells in 14% of the cases on day +60 and 22% on day +90. Finally, a small proportion of patients (6% and 7% at days +60 and +90) displayed an intriguing complete chimerism in CD3+ cells yet mixed WB chimerism. None of these features appeared associated to disease lineage (lymphoid or myeloid) nor cord blood allo-SCT. Interestingly, of the 27 grafts with myeloablative conditioning, only 14 had full WB/CD3+ engraftment on day +60 or +90, and thus all 27 were retained for the study. None of the four WB/CD3 chimerism combinations at the two times considered had an impact on DFS in this cohort. Surprisingly, although full or mixed WB chimerism had no impact on DFS and OS at days +60 and +90, the presence of a mixed CD3+ chimerism (vs full) at day+90 was associated with a significantly better OS (median: 5.8 months years [95%CI: -not reached] versus 3.1 years [95%CI: 2.2- 3.1]; p=0.025). CD3+ chimerism at day+60 has no impact on OS. All HG resulted in full CD3+ chimerism at both time points compared to non HG (100% vs 52%, p<0.0001). The same was almost true when considering PTCY procedures: 90% at day+60 and 92% at day +90. Of note, there was no influence on DFS nor OS of WB or CD3+ chimerism status when considering only HG or PTCY grafts vs others in this series. Discussion: In this large series, early WB chimerism status did not predict outcome. Surprisingly, mixed CD3+ chimerism at day+90 appears to be significantly associated with a longer OS, suggesting that remaining recipient memory lymphocytes could be beneficial. This result has to be confirmed prospectively. It remains also to define the place of donor lymphocyte infusions (DLI) to prevent relapse in patients with full or mixed CD3+ chimerism post-transplant (analyses of DLI received in our patients are on-going). Disclosures Moreau: Bristol-Myers Squibb: Honoraria; Takeda: Honoraria; Novartis: Honoraria; Amgen: Honoraria; Janssen: Honoraria, Speakers Bureau; Celgene: Honoraria.
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Jiménez-Pulido, Iván Jesús, Daniel Rico, Daniel De Luis, and Ana Belén Martín-Diana. "Combined Strategy Using High Hydrostatic Pressure, Temperature and Enzymatic Hydrolysis for Development of Fibre-Rich Ingredients from Oat and Wheat By-Products." Foods 13, no. 3 (January 24, 2024): 378. http://dx.doi.org/10.3390/foods13030378.

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Wheat bran (WB) and oat hull (OH) are two interesting undervalued cereal processing sources rich in total dietary fibre (TDF) and other associated bioactive compounds, such as β-glucans and polyphenols. The aim of this study was to optimise a combination chemical (enzymes) and physical (high hydrostatic pressure-temperature) strategies to increase the bioaccessibility of bioactive compounds naturally bound to the bran and hull outer layers. WB and OH were hydrolysed using food-grade enzymes (UltraFloXL and Viscoferm, for WB and OH, respectively) in combination with HPP at different temperatures (40, 50, 60 and 70 °C) and hydrolysis either before or after HPP. Proximal composition, phytic acid, β-glucans, total phenolics (TPs) and total antioxidant activity (TAC) were evaluated to select the processing conditions for optimal nutritional and bioactive properties of the final ingredients. The application of the hydrolysis step after the HPP treatment resulted in lower phytic acid levels in both matrices (WB and OH). On the other hand, the release of β-glucan was more effective at the highest temperature (70 °C) used during pressurisation. After the treatment, the TP content ranged from 756.47 to 1395.27 µmol GAE 100 g−1 in WB, and OH showed values from 566.91 to 930.45 µmol GAE 100 g−1. An interaction effect between the temperature and hydrolysis timing (applied before or after HPP) was observed in the case of OH. Hydrolysis applied before HPP was more efficient in releasing OH TPs at lower HPP temperatures (40–50 °C); meanwhile, at higher HPP temperatures (60–70 °C), hydrolysis yielded higher TP values when applied after HPP. This effect was not observed in WB, where the hydrolysis was more effective before HPP. The TP results were significantly correlated with the TAC values. The results showed that the application of optimal process conditions (hydrolysis before HPP at 60 or 70 °C for WB; hydrolysis after HPP at 70 °C for OH) can increase the biological value of the final ingredients obtained.
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Fiorilli, Giovanni, Federico Quinzi, Andrea Buonsenso, Giusy Casazza, Luigi Manni, Attilio Parisi, Alfonso Di Costanzo, Giuseppe Calcagno, Marzia Soligo, and Alessandra di Cagno. "A Single Session of Whole-Body Electromyostimulation Increases Muscle Strength, Endurance and proNGF in Early Parkinson Patients." International Journal of Environmental Research and Public Health 18, no. 10 (May 20, 2021): 5499. http://dx.doi.org/10.3390/ijerph18105499.

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Parkinson’s disease (PD) patients lead a sedentary lifestyle, being unable or unwilling to exercise conventionally, due to physical and mental limitations. The aim of this study was to assess the acute effects of a single session of whole-body electromyostimulation (WB-EMS) on the physical performances and serum levels of the neurotrophic factors in PD patients. Ten subjects (aged 72.60 ± 6.82) underwent 20 min of physical activity with superimposed WB-EMS and, after four weeks, the same protocol with no WB-EMS. WB-EMS was conducted with intermittent stimulation, with 4 s WB-EMS/4 s rest, at 85 Hz, 350 μs. A physical fitness assessment and blood samples collection, to evaluate neurotrophic factors’ levels (BDNF, FGF21, proNGF, mNGF), were collected before and after the intervention. The RM-ANOVA showed significant improvements in sit-to-stand (p < 0.01), arm curl (p < 0.01), handgrip (p < 0.01) and soda pop test (p < 0.01) after the WB-EMS intervention. Higher proNFG serum levels were observed in the WB-EMS condition compared to the no WB-EMS after 60 min post-intervention (p = 0.0163). The effect of WB-EMS confirmed the electrostimulation ability to modulate the proNGF quantity. The positive impact of the WB-EMS protocol on physical functioning, and eye–hand coordination, makes this intervention a promising strategy to improve motor and non-motor symptoms in PD patients.
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Friesen, Brian J., Martin P. Poirier, Dallon T. Lamarche, Andrew W. D’Souza, Jung-Hyun Kim, Sean R. Notley, and Glen P. Kenny. "Postexercise whole-body sweating increases during muscle metaboreceptor activation in young men." Applied Physiology, Nutrition, and Metabolism 43, no. 4 (April 2018): 423–26. http://dx.doi.org/10.1139/apnm-2017-0675.

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We assessed the effect of metaboreceptor activation on whole-body evaporative heat loss (WB-EHL) in 12 men (aged 24 ± 4 years) in the early-to-late stages of a 60-min exercise recovery in the heat. Metaboreceptor activation induced by 1-min isometric-handgrip (IHG) exercise followed by 5-min forearm ischemia to trap metabolites increased WB-EHL by 25%–31% and 26%–34% during the ischemic period relative to IHG-only and control (natural recovery only), respectively, throughout recovery. We show that metaboreceptor activation enhances WB-EHL in recovery.
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8

Greene, Sarah E., Yuefang Huang, Kurt C. Curtis, Christopher L. King, Peter U. Fischer, and Gary J. Weil. "IgG4 antibodies to the recombinant filarial antigen Wb-Bhp-1 decrease dramatically following treatment of lymphatic filariasis." PLOS Neglected Tropical Diseases 17, no. 6 (June 7, 2023): e0011364. http://dx.doi.org/10.1371/journal.pntd.0011364.

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Background Lymphatic filariasis (LF) is a neglected tropical disease and a major cause of chronic disability. Improved diagnostic tests are needed because of long-term persistence of anti-filarial antibodies or circulating filarial antigenemia after treatments that clear microfilaremia. Here, we assess changes in levels of antibodies to the recombinant filarial antigens Wb-Bhp-1, Wb123, and Bm14 after anti-filarial treatment. Methodology/principal findings IgG4 antibodies to recombinant filarial antigens were assessed by ELISA. We tested serial plasma samples from a clinical trial in Papua New Guinea. Before treatment, 90%, 71% and 99% of participants had antibodies to Wb-Bhp-1, Wb123, and Bm14, respectively. Antibodies to Wb-Bhp-1 and Wb123, but not Bm14, were significantly higher in participants with persistent microfilaremia 24 months after treatment. Antibodies to all three antigens declined significantly by 60 months after treatment with ivermectin, diethylcarbamazine and albendazole despite circulating filarial antigen in 76% of participants. By 60 months follow up, antibodies to Wb-Bhp-1, Wb123, and Bm14 were detected in 17%, 7% and 90% of participants, respectively. Antibodies to Wb-Bhp-1 also declined more rapidly after treatment than antibodies to Bm14 in samples from a clinical trial conducted in Sri Lanka. We also tested archived serum samples from people living in filariasis-endemic communities in Egypt with different infection profiles. Antibodies to Wb-Bhp-1 were detected in 73% of microfilaremic people, 53% of amicrofilaremic people with circulating filarial antigen, and 17.5% of endemic individuals without microfilaria or circulating filarial antigen. Tests performed with legacy samples from India showed that few people with filarial lymphedema had antibodies to these recombinant antigens. Conclusions Antibodies to Wb-Bhp-1 and Wb123 are more closely correlated with persistent microfilaremia than circulating filarial antigenemia or antibodies to Bm14, and they clear more rapidly after anti-filarial treatment. Additional studies are needed to assess the value of Wb-Bhp-1 serology as a tool for determining the success of LF elimination efforts.
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Kemmler, Wolfgang, Anja Weissenfels, Michael Bebenek, Michael Fröhlich, Heinz Kleinöder, Matthias Kohl, and Simon von Stengel. "Effects of Whole-Body Electromyostimulation on Low Back Pain in People with Chronic Unspecific Dorsal Pain: A Meta-Analysis of Individual Patient Data from Randomized Controlled WB-EMS Trials." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/8480429.

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In order to evaluate the favorable effect of whole-body electromyostimulation (WB-EMS) on low back pain (LBP), an aspect which is frequently claimed by commercial providers, we performed a meta-analysis of individual patient data. The analysis is based on five of our recently conducted randomized controlled WB-EMS trials with adults 60 years+, all of which applied similar WB-EMS protocols (1.5 sessions/week, bipolar current, 16–25 min/session, 85 Hz, 350 μs, and 4–6 s impulse/4 s impulse-break) and used the same pain questionnaire. From these underlying trials, we included only subjects with frequent-chronic LBP in the present meta-analysis. Study endpoints were pain intensity and frequency at the lumbar spine. In summary, 23 participants of the underlying WB-EMS and 22 subjects of the control groups (CG) were pooled in a joint WB-EMS and CG. At baseline, no group differences with respect to LBP intensity and frequency were observed. Pain intensity improved significantly in the WB-EMS (p<.001) and was maintained (p=.997) in the CG. LBP frequency decreased significantly in the WB-EMS (p<.001) and improved nonsignificantly in the CG (p=.057). Group differences for both LBP parameters were significant (p≤.035). We concluded that WB-EMS appears to be an effective training tool for reducing LBP; however, RCTs should further address this issue with more specified study protocols.
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Dominelli, Paolo B., Yannick Molgat-Seon, Derek Bingham, Philippa M. Swartz, Jeremy D. Road, Glen E. Foster, and A. William Sheel. "Dysanapsis and the resistive work of breathing during exercise in healthy men and women." Journal of Applied Physiology 119, no. 10 (November 15, 2015): 1105–13. http://dx.doi.org/10.1152/japplphysiol.00409.2015.

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We asked if the higher work of breathing (Wb) during exercise in women compared with men is explained by biological sex. We created a statistical model that accounts for both the viscoelastic and the resistive components of the total Wb and independently compares the effects of biological sex. We applied the model to esophageal pressure-derived Wb values obtained during an incremental cycle test to exhaustion. Subjects were healthy men ( n = 17) and women ( n = 18) with a range of maximal aerobic capacities (V̇o2 max range: men = 40-68 and women = 39–60 ml·kg−1·min−1). We also calculated the dysanapsis ratio using measures of lung recoil and forced expiratory flow as index of airway caliber. By applying the model we found that the differences in the total Wb during exercise in women are due to a higher resistive Wb rather than viscoelastic Wb. We also found that the higher resistive Wb is independently explained by biological sex. To account for the known effect of lung volumes on the dysanapsis ratio we compared the sexes with an analysis of covariance procedures and found that when vital capacity was accounted for the adjusted mean dysanapsis ratio is statistically lower in women (0.17 vs. 0.25 arbitrary units; P < 0.05). Our collective findings suggest that innate sex-based differences may exist in human airways, which result in significant male-female differences in the Wb during exercise in healthy subjects.
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Books on the topic "Wb 60"

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George, Khushf, ed. Handbook of bioethics: Taking stock of the field from a philosophical perspective. Dordrecht: Kluwer Academic, 2004.

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1956-, Nerlich Brigitte, Elliott Richard 1982-, and Larson Brendon, eds. Communicating biological sciences: Ethical and metaphorical dimensions. Burlington, VT: Ashgate, 2009.

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Bioethics: Principles, issues, and cases. 2nd ed. New York: Oxford University Press, 2013.

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Intervention and Reflection: Basic Issues in Bioethics. Wadsworth, 2013.

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Intervention and Reflection: Basic Issues in Bioethics, Concise Edition. Wadsworth, 2012.

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Intervention and Reflection: Basic Issues in Bioethics. Wadsworth, 2016.

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(Editor), Jennifer Gunning, and Soren Holm (Editor), eds. Ethics, Law And Society (Ethics, Law and Society). Ashgate Publishing, 2005.

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Bioethics: A Primer for Christians. Eerdmans Publishing Company, William B., 2020.

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Bioethics: A Primer for Christians. Eerdmans Publishing Company, William B., 2004.

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Bioethics: A Primer for Christians. Eerdmans Publishing Company, William B., 2004.

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Conference papers on the topic "Wb 60"

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Lall, Pradeep, and Sungmo Jung. "Reliability Evaluation of Cu-Al WB in High Temperature and High Current Applications." In ASME 2021 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/ipack2021-75890.

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Abstract High reliability harsh environment applications necessitate a better understanding of the acceleration factors under operating stresses. Automotive electronics has transitioned to the use of copper wire for first level interconnects. A number of copper wire formulations have emerged including palladium coated copper and gold-flash palladium coated copper. The corrosion reliability of copper wire bonds in high temperature conditions is not yet fully understood. The EMC used to encapsulate chips and interconnects can vary widely in formulation, including pH, porosity, diffusion rate, composition of contaminants and contaminant concentration. To realistically represent the expected wirebond reliability, there is need for a predictive model that can account for environmental conditions, operating conditions, and exposure to EMCs. In this paper, different EMCs were studied in a high-temperature-current environment with temperature range of 60°C–100°C under current of 0.2A–1A. The diffusion kinetics based on the Nernst-Planck Equation for migration of the chlorine ions has been coupled with the Butler-Volmer equation for corrosion kinetics to create a Multiphysics model. Polarization curves have been measured for copper, aluminum and intermetallics under a number of pH values, and chlorine-ion concentrations. Tafel parameters have been extracted through measurements of the polarization curves.
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S. V., Shalygin, and Russkikh G. S. "EXPERIMENTAL VERIFICATION OF AN ALGORITHM FOR OPTIMIZING THE INTERNAL STRUCTURE OF SAMPLES OBTAINED BY FDM AND SLA PRINTING METHODS OPERATING UNDER THREE-POINT BENDING CONDITIONS." In Mechanical Science and Technology Update. Omsk State Technical University, 2022. http://dx.doi.org/10.25206/978-5-8149-3453-6-2022-60-68.

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The work is devoted to the description of the algorithm for the formation of the internal structure of the product obtained using FDM technology considering the stress-strain state, based on the use of isosurfaces of equivalent stresses obtained on a pre-calculated stress-strain state of the sample in an elastic isotropic formulation. The main purpose of the work is to verify the previously presented algorithm for optimizing the internal structure of 3D printed samples obtained by FDM printing, operating under three-point bending conditions. The data obtained earlier are based on numerical experiments using finite element analysis methods in Ansys WB. In this paper, the assumption made for numerical experiments are consistently verified for each conditional step of the optimization algorithm. As well as experimental tests of optimized samples with isotropic and anisotropic properties
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Mariani, G., R. Ghirardini, P. Verani, F. Mandelli, G. B. Rossi, M. P. Mannucci, and S. Butto. "ANTI-HIV AFTER HEATED CLOTTING FACTOR CONCENTRATES IN HEMOPHILIACS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643971.

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In Italy, heated concentrates became the only source of hemophilia therapy since July 1985, when a government act enforced their use instead of nonheated concentrates. Since then 63 anti-HIV seronegative hemophiliacs treated with heated concentrates were followed-up prospectively, focusing on the development of anti-HIV. Anti-HIV (documented by persistent positivity for ELISA and WB) occurred in 6 patients who had no other risk factor for HIV infection. For 3, anti-HIV was first found in Sept., Oct. or Nov. 1985 i.e. within 4 months of the last infusion of unheated concentrates (July 1985). For another patient, anti-HIV was found in Sept. 1986, but no other sample was available after the last negative test (Nov. 1985). For these 4 cases, therefore, we cannot exclude that seroconversions are due to nonheated concentrates used until July 1985. For 2 patients, however, anti-HIV occurred in July 1986, i.e. 11 months after change to heated concentrates. For both a hemophilia A patient (treated exclusively with a concentrate dry-heated for 72 hr at 68°C) and a hemophilia B patient (treated with both a steam-heated concentrate and a concentrate dry-heated for 72 hr at 68°C) the last seronegativities were found in March 1986, i.e. 7.0 and 7.5 months after commencing the use of heated concentrates or 3.5 and 4.0 months before the first seropositivity. The overwhelming majority of heated concentrates were prepared from non-donor-screened plasma. In conclusion, two anti-HIV occurred in previously seronegative patients treated exclusively with heated concentrates. Intensity and duration of concentrate exposure to heating were greater than those for the commercial dry-heated concentrate (60°C for 30 hours) that caused two reported seroconversions.
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