Dissertations / Theses on the topic 'Water quality – Ontario'

A two-year field study was carried out in eastern Ontario to investigate the effects of water table management (WTM) on water quality and crop yields. Corn (Zea mays L.) and soybean (Glycine max Merr.) were planted in alternate strips across the three treatments of 50 cm controlled water table (CWT), 75 cm CWT and free drainage (FD). Drainflow volume and nitrate-N concentration of the drainage water were measured. Soil samples were collected and analysed for total N, P, K, available N, soil moisture and organic matter levels. Chlorophyll-meter readings and plant harvest parameters were also measured. Rainfall, soil and air temperatures were recorded throughout the growing seasons.
The obtained data show that in 1995, the CWT plots significantly increased total drainflow, as compared to FD. In 1996, overall drainflow and nitrate concentrations were significantly reduced. Both the corn and soybean yields were higher with WTM than with FD for both years. (Abstract shortened by UMI.)

Forty-seven riffle zones from 21 streams of Eastern Ontario and Western Quebec were sampled in 1998 to describe how characteristics of the benthic invertebrate assemblage (abundance, taxa richness and size distribution) varied as a function of water quality parameters (conductivity, TP, SRP, TSS, NO3+NO2, NH3, TKN, Cl-, SO42-) along a gradient of watershed development. A principal components analysis on water quality parameters revealed that there were two groups of correlated water quality variables that explained the majority of the variability among sites. The first group of variables included chloride, sulfate, nitrate+nitrite and conductivity and represented a gradient of urbanization while the second group represented nutrients and included: soluble reactive phosphorus, total phosphorus, ammonia, total suspended solids and total Kjeldahl nitrogen. Simple and multiple regression models predicting invertebrate assemblage characteristics were fitted using water quality principal components scores as independent variables. Overall, invertebrate assemblage characteristics were related to both groups of water quality variables. Abundances per taxon and size classes generally increased with increased nutrients, and overall abundance and the ratio of abundances of sensitive to tolerant taxa declined with increasing chloride, sulfate, nitrate+nitrite and conductivity. Existing information suggests that the water quality gradient found in these streams is more a reflection of anthropogenic sources than the result of geological differences. Therefore, it appears that human activities affect the distribution and abundance of invertebrates in this region. However our models did not explain a good proportion of the variability. It would seem that stream invertebrates of the Ottawa valley are also affected by other parameters that have yet to be identified.

Over the last 150 years the 'natural' landscape in southeastern Ontario (as in much of the world) has undergone a dramatic transformation due, in large part, to widespread deforestation, wetland destruction and degradation, and increased agricultural activity. The negative impacts of such land-use modifications may include declines in wetland water quality and biodiversity. Here, I develop models to predict the effects of adjacent land-use on wetland water quality, and amphibian and plant diversity using indices of land-use intensity such as, forest cover, road density, building density, fertiliser application, livestock density etc. I find negative relationships between land-use intensity and wetland water quality, and amphibian and plant species richness. The strongest relationships tend to be with forest cover, however, other important variables include wetland size, road density, and the proportion of adjacent lands that is wetland. Moreover, there are complex interactions among variables. For instance, part of the effect of forest cover on plant and amphibian species richness may be indirect, through effects on wetland water quality but there are also effects of forest cover on both plants and amphibians that are independent of wetland water quality. In a conservation context, one important question is 'what is the scale of adjacent land-use effects?' I find that landscape modifications up to 2000--4000 meters from a wetland edge have the strongest correlations with wetland water quality and amphibian species richness while, land-uses 250--400 meters from the wetland edge are most strongly correlated with plant species richness. The conservation implication is that the current Ontario Wetland Policy which (1) evaluates and protects wetlands on a site-by-site basis and, (2) regulates adjacent land-use out to 120 meters from the wetland edge, is not likely to ensure long-term protection of wetland water quality and biodiversity in Ontario.

The purpose of this research is to describe and analyze the present and potential role of non-governmental organizations (NGO's) in the management of agricultural soil erosion and associated water quality decline (ASEWQ), in Ontario. A series of five tasks are associated with this research purpose. These tasks include: the cataloguing of all agricultural NGO's in Ontario; the assessment of this NGO population's vertical interaction; the assessment of the NGO population's horizontal spread; analysis of NGO mobilization potential in the area of ASEWQ management; and finally the characterization of degree of NGO favourability towards, and involvement in, ASEWQ management. Research has led to the discovery of a very large and complex population of agricultural organizations in Ontario. The horizontal distribution of this population closely reflects the geographic configuration of agricultural activity in the province. The vertical distribution reveals a high degree of organizational affiliation and networking which gives the grassroots indirect access to provincial policy influence. Despite the size, complexity and general favourability of this population towards soil conservation, few NGO's have suitable potential to mobilize on the ASEWQ issue. As a result, only a few strategically placed and organized NGO's have any involvement in controlling agricultural soil erosion. In conclusion, this research offers a series of recommendations to provincial government agricultural policy makers and future research avenues. (Abstract shortened by UMI.)

Phosphorus, a mineral nutrient, is an essential element in aquatic systems. It is only available for biological activity in the form of orthophosphate and soluble restrictive phosphate. Eutrophication, caused by nutrient enrichment, is a problem in many freshwater systems, which results in increased algal blooms, anoxic conditions, and consequently, biodiversity loss and ecosystem failure. Low dissolved oxygen levels trigger the release of sediment bound phosphorus, which reinforces eutrophication. Nutrients in aquatic systems are provided by point and non-point sources and these sources can be affected by several factors, including population, land-use, and climate change. There are many long-term historical phosphorus studies on rivers, but there are very few that are conducted on the Grand River watershed and none that look at factors that might be driving the phosphorus loadings. The Grand River watershed, located in Ontario, Canada, is a highly agricultural watershed with a growing population of approximately one million. It has experienced eutrophication, which has led to excessive production of cyanobacteria and regions of hypoxia. In this study, historical phosphorus concentration data (Total Phosphorus, Soluble Reactive Phosphorus, and Particulate Phosphorus) in five sites along the Grand River were analyzed temporally and spatially from 1965 to 2010 in the upper, middle, and lower parts of the watershed. The Particulate Phosphorus was calculated by subtracting SRP from TP. Several other data such as climate, land-use, geology, and population were also explored and considered as possible factors that may have influenced the trends over time. TP, SRP, and PP average flow weighted concentrations and fluxes were calculated in 2-6 year intervals. SRP load was higher prior to the early 1970???s, declined in the 1970???s, was more stable in the 1980???s and 1990???s, and increased in the 2000???s. The initial decrease in SRP in the early 1970???s was likely due to the phosphorus ban in detergents in 1973 that was implemented over several years. The constant SRP loadings in the 1980???s and 1990???s, despite population and urban development growth, may have been due to upgrades in waste water treatment plants during that time period. The recent increase in phosphorus in more recent years coincides with a large increase in the number of livestock in the 2000???s and population growth. SRP and PP loads increase from upstream to downstream regions are likely due to nutrient accumulation by the river. The higher loads and concentrations of SRP in the CGR is expected because the region is highly urbanized and contains most of the tile drainage in the watershed.

This thesis summarizes the efforts of two years of field investigation and water quality data analysis. With a focus on construction sites, background monitoring was carried out at two sites and data from a third, active site was also included for analysis. The water quality data was used to estimate event-based sediment yield from each location, and continuously-collected rainfall, water level and turbidity data was used to calibrate an event-based hydrologic model (SEDCAD). Based on the results of this research and the outputs of the calibrated model, an event-based sediment yield equation calibrated for Southern Ontario conditions is presented in conjunction with an IDF design tool. The IDF design tool can be used to effectively size and site construction-phase erosion and sediment controls before shovels break ground. The regulatory framework by which such controls are assessed is also discussed, and improvements to existing stormwater management guidelines are proposed.

A two-part paleolimnological study was conducted to examine changes in historical and modern water-quality in Lake of the Woods (LoW) in response to multiple stressors, such as climate change and shoreline residential development. Changes in diatom assemblages were analyzed in the modern and pre-industrial sediment intervals of 17 study sites, and in high-resolution 210Pb-dated sedimentary records from three northwestern bays (Clearwater, Poplar, and White Partridge bays). Patterns in diatom assemblage changes revealed pronounced and synchronous shifts over the last ca. 150 years. The most notable shift in the diatom community structure (~1970 AD) was characterized by an overall shift towards a higher relative abundance of small, centric Cyclotella taxa and planktonic, pennate diatoms (e.g., Asterionella formosa, Fragilaria crotonensis), and a corresponding lower relative abundance of heavily silicified Aulacoseira taxa and small benthic Fragilaria taxa and Achnanthes taxa. Lakewater total phosphorus (TP) concentrations were inferred from sedimentary diatom assemblages. DI-TP reconstructions revealed either no change or a decline in DI-TP since pre-industrial (pre-1850) times at majority (88%) of the top-bottom study sites, and no distinct directional change over the past ca. 150 years at the northwestern bays. Therefore, we concluded that TP was not an important driver of the floristic changes we observed. Chl-a trends indicate that primary production increased during the last ca. 100 years, likely tracking increases in microbial blooms. Changes in diatom assemblage composition and primary productivity have occurred during a period of substantial warming for this region of northwestern Ontario. Strong correlations (r >0.50, p<0.005) between diatom compositional changes, chl-a trends, and local air temperature records and lake-ice phenology suggest that climate-induced changes in lakewater properties may have been key factors driving the observed changes. From these data, we conclude that climate warming, rather than changing shoreline development and TP changes, has had the most pronounced effect on algal communities in the LoW. Estimates of pre-impact lake trophic status can aid in setting realistic mitigation targets for lakes impacted by multiple stressors. Therefore, paleolimnological studies comparing pre- and post-disturbance algal assemblages, such as the investigations we have conducted, are of interest from a lake management perspective.
Thesis (Master, Biology) -- Queen's University, 2010-05-28 13:38:59.472

In Canada, as in many other affluent nations, private well water consumers remain at risk for gastrointestinal (GI) illness due to fecal contamination of groundwater. There have been numerous documented outbreaks of GI illness related to contaminated drinking water. While the general risk to well water consumers has been established, the risk in southern Ontario is poorly understood. As a preliminary step towards understanding this risk, a study of Escherichia coli (E. coli) contamination in private well water was undertaken. Spatial scan statistics were employed to determine the extent of contamination for over 30,000 private wells in southeastern Ontario between 2008 and 2012, inclusive. This analysis revealed one large, temporally stable elevated risk region, and three significant smaller regions within it. The methodology utilized in the primary investigation was then applied to a 2012 dataset for all of southern Ontario, resulting in the identification of three regions of elevated risk. The presence of E. coli, a traditional fecal indicator organism, indicates lack of water potability. To provide knowledge regarding the origins of fecal contamination in southeastern Ontario, a molecular microbial source tracking (MST) study was undertaken. A quantitative real-time Bacteroidales PCR assay specifically targeting human, bovine, and general (specific to 10 hosts) was optimized and applied to 716 private well water samples. Almost half of the samples showed evidence of human fecal contamination, whereas only 13% contained evidence of bovine fecal contamination. Approximately one quarter of well samples tested positive for the general host Bacteroidales assay, with an additional one quarter testing negative for all MST assays. Additionally, spatial scan statistics revealed a region of human-sourced contamination, which geographically corresponded with the E. coli contamination cluster for the same study year. The presence of E. coli contamination clusters among private wells reveals an at-risk group of well water consumers. As such, public health practitioners may use this information to target well stewardship programs in higher risk regions. Humans were the predominant contributors of fecal contamination to private wells within the primary study region. These findings may enable future preventative measures by providing insight into the true origins of groundwater fecal pollution.
Thesis (Master, Pathology & Molecular Medicine) -- Queen's University, 2014-06-02 14:50:16.685

The dominant social values and perceptions of the 1970s, which guided the development of institutional and procedural frameworks for risk policy making in Canada, have changed since that time. Over the last decade, the public has signalled problems with the perceived legitimacy of frameworks for risk policy making which involve closed negotiations between government and industry, and which rely heavily on the recommendations of a select group of scientific experts. Consequently, decision makers are adopting participatory policy making processes in attempts to restore public perceptions of legitimacy. This thesis examines the nature of public participation in risk policy making and evaluates the extent to which this type of approach, as it is designed in the context of a case study, addresses problems of legitimacy. On analyzing submissions from a public consultation on a drinking water guideline for tritium, it was found that decision makers failed to recognize the prevalence of legitimacy concerns, and thus did not design the process of public participation to address these issues. In employing a process of public involvement which resulted in no significant redistribution of influence over decision making, and in assuming that public concerns could be allayed by providing more technical information regarding risk assessment techniques, decision makers failed to recognize the changing social context for decision making on risk issues. If present trends in public values and perceptions continue, policy domains like that of radiation health protection, which employ closed, science-based decision processes, will be confronted with more pronounced problems of legitimacy which call for radical changes to both the social and the scientific assumptions which underlie risk policy making.

Vegetated roof technologies are increasingly being adopted as treatment measures to mitigate the effects of urban stormwater. A mass balance approach was used to assess the wet weather performance of a vegetated roof on the top of city hall in Waterloo, Ontario. Vegetated and control roof sections were instrumented to measure precipitation inputs, storage and outflow for 18 storm events from June to October, 2006. Concentrations of suspended solids (SS), total phosphorus (TP), soluble reactive phosphorus (SRP), copper (Cu), zinc (Zn), chromium (Cr) and cadmium (Cd) in precipitation and roof (vegetated and control) runoff were measured. A total of 155.6 mm of rain fell during the study period. The vegetated roof retained 64.5 mm (41.5%) of the total rainfall while the control roof retained ~ 5.1 mm (3.3 %). For individual rain events, the vegetated roof retained an average of 3.5 mm (47.6 %) while the control roof retained ~ 0.3 mm (4.7 %). Water retention varied with storm size, season and was influenced by wetting history. The vegetated roof retained 80.6 % of precipitation for light storm events (≤ 3.5 mm) and 34.9 % for large storm events (> 3.5 mm). The control roof retained 7.6 % light storm events and 3.7 % for large storm events. Water quality from the vegetated roof did not show significant improvement as only Zn concentrations in runoff from the vegetated roof were significantly lower than that measured in runoff from the control roof. Concentrations of SS, Cu, Cr and Cd in vegetated roof runoff were relative to concentrations in rainfall and control roof runoff and TP and SRP concentrations were significantly higher than that in rainfall or control roof runoff. Results gained from this study may assist people in planning and stormwater management by providing insight into the monitoring, development and application of new stormwater controls.

The increased use of synthetic nitrogen fertilizers since the early 1900s has resulted in greater food production but also problems with nitrogen pollution in freshwaters. Nitrate (NO3-) is a common pollutant in rivers and groundwater in agricultural watersheds; the drinking water limit in Canada is 10 mg N/L. Microbial processing of NO3- and ammonium (NH4+) can produce nitrous oxide (N2O), a potent greenhouse gas responsible for about 5% of the greenhouse effect. Rivers provide a complex environment, where a variety of redox conditions, available substrates and microbial populations can co-exist on small spatial and temporal scales. Therefore, many questions remain about N cycling in river environments. N2O is produced during anoxic microbial NO3- or NO2- reduction to N2 (denitrification) and oxic microbial NH4+ oxidation to NO3- (nitrification). A significant portion (~25%) of global anthropogenic N2O is produced in rivers and estuaries, but mechanisms are not clear and predictability is poor. The United Nations Intergovernmental Panel on Climate Change (IPCC) provides default equations for calculating N2O emission estimates, in which annual NO3- loading to rivers is positively linearly related to N2O emissions. However, it is unclear how sound these linear relationships are and if measured N2O emissions are similar to IPCC estimates. The Grand River watershed is the largest in southern Ontario. Nutrient discharge to the Grand River is high due to extensive agriculture and high urban populations. The river often has a hypoxic water column due to high community respiration in summer. However, although nitrogen pollution is significant, N cycling is not well understood in the river. This thesis shows that NO3- and NH4+ do not typically change on the diel scale, with the exception of two sites downstream of wastewater treatment plants (WWTPs). However, N2O concentration changes dramatically. N2O concentrations are higher at night and lower during the day for most sites, but are reversed at very low-nutrient sites. N2O is therefore a sensitive indicator of changes in N cycling that may not be evident from NO3- and NH4+ concentrations or stable isotope ratios. Additionally, this work shows the importance of having a sampling design that captures diel variability in N2O. Previous work in rivers and streams worldwide focused on the appropriate N2O:NO3- ratio used to predict N2O emissions. In contrast, this thesis shows that there is a significant but very weak relationship between instantaneous N2O emissions and NO3- concentrations. However, there is a much stronger negative exponential relationship between DO and N2O. Annual N2O emissions tripled between 2006 and 2007 but NO3- masses in the river were only 10% higher, likely because river levels were lower and anoxia more prevalent in 2007. This research suggests that the IPCC needs a new conceptual model for N2O-NO3- relationships in rivers. N2O is produced in rivers, partially due to microbial processing of NO3- and NH4+ from WWTP effluent. However, WWTP effluent may also include dissolved N2O and CH4 but this previously had not been directly quantified. It was also unclear if stable isotopic ratios of NH4+, NO3-, N2O and CH4 in WWTP effluent were distinct from river sources and could be used for effluent tracing. N2O emissions from three WWTPs in the Grand River Watershed were measured over 24 hours in summer and winter. N2O emissions were similar to direct emissions from WWTPs but CH4 emissions were about an order of magnitude lower than direct WWTP emissions. This is a previously-ignored source of N2O and CH4 to the atmosphere. While stable isotopic ranges of NO3- and NH4+ were not always distinct from river sources, ??15N-N2O, ??18O-N2O and ??13C-CH4 were distinct, making them potentially useful tracers of WWTP effluent in rivers. N2O isotopic signatures may help determine production and removal processes in rivers, but isotopic effects of the major production pathway, denitrification, have not been characterized for river sediments. This was addressed by preparing anoxic laboratory incubations of river sediment from two sites (non-urban and urban) in the Grand River and measuring stable isotopic effects of N2O production via denitrification. Stable isotopic fractionations were similar to published values but, surprisingly, strongly negatively correlated to production rate, even though NO3- substrate was plentiful. This novel finding suggests that N2O reduction resulting in isotopic effects is more prevalent in high-substrate systems than previously thought, and that N2O reduction may be inhibited by high NO3- or NO2- or by lags in N2O reductase activity in high N2O-production incubations. This could explain why N2O emissions from the Grand River are lower than predicted by IPCC equations, which assume that N2O:(N2O+N2) ratios produced by denitrification are constant. Concern about NO3- export to freshwater lakes and to oceans is growing, but the role of large, eutrophic rivers in removing watershed NO3- loading via denitrification and biotic assimilation is not clear. To understand how much NO3- the Grand River receives, and how much it removes annually, a NO3- isotope mass balance for the Grand River was created. The river denitrified between 0.5% and 17% of incoming NO3-, less than the 50% suggested by the IPCC. This is surprising, as the river is well mixed, has moderate to high NO3- concentrations, experiences hypoxia (promoting denitrification), and has extensive biomass (biofilm and macrophytes) that assimilate N. However, the river???s short residence time (~3 days not counting reservoirs), organic carbon-poor sediment and mineralization of organic matter could contribute to low denitrification rates. These findings suggest that denitrification rates in rivers worldwide could be lower than previously estimated. Although error was high, most ??15N-NO3- values for losses were in the expected range for denitrification and most ??15N-NO3- values for gains were within ranges from tributaries, WWTP effluent and groundwater measured in the watershed. The model suggests that 68% to 83% of N loads to the watershed are lost before entering the Grand River, and 13% is exported to Lake Erie, leaving 5 to 19% lost in the Grand River from a combination of denitrification, assimilation and storage. These findings suggest that large rivers are much less efficient in denitrification than other locations in watersheds such as small streams, ponds, groundwater and riparian zones. They also indicate that agricultural NO3- loading is much higher than WWTP effluent, suggesting that N management strategies should focus on agricultural runoff and groundwater. Given that N2O:NO3- relationships are weak and non-linear in the Grand River, a new conceptual model for N2O:NO3- relationships is presented. First, the Grand River dataset was supplemented with data from high-oxygen streams in southern Ontario. Regression tree analysis shows a weak relationship between NO3- and N2O in these streams with no other factors (temperature, DO, NH4+, TP, DOC, etc.) improving fit. A conceptual model was then created, which posits that N2O emission variability (between and within sites) increases with NO3- concentration when NO3- concentrations are above the threshold for NO3- limitation. The global dataset does not dispute this model, though a NO3- threshold was not clear. The lack of sites with both high NO3- and high N2O may indicate a paucity of research on eutrophic sites. Alternatively, high NO3- may indicate oxic conditions (i.e. little to no denitrification to remove it) which are incompatible with very high N2O emissions. In this case, the conceptual model can be modified such that N2O variability decreases when NO3- > ~ 4 mg N/L. The work also shows that low DO consistently results in high N2O emissions but high temperatures result in a very large range of N2O emissions. This approach allows N2O emissions, which have very high variability and are difficult to predict, to be constrained to likely ranges.

In response to sulphur dioxide emission reductions in North America and Europe, there has been a recent shift in research focus towards understanding ecosystem recovery. Evidence for reversibility in the effects of acidification on lake ecosystems within North America has been, for the most part, constrained to a single locality (Sudbury, Ontario). Lakes in a fume-kill area near Wawa, Ontario, present a new and rare opportunity for studying potentially rapid chemical and biological recovery patterns from extreme acidification. Several lakes acidified (pH ~3) during a period of local iron sintering from 1939 to 1998, and although minimal historical data are available, striking biological recovery has recently been observed. This study used paleolimnological techniques to track diatom (class Bacillariophyceae) responses to historical water quality changes in five fume-kill lakes near Wawa. Prior to the onset of iron sintering, the sediment-recorded diatom assemblages were dominated by species that are typically found in circumneutral or slightly alkaline lakes. Following the known occurrence of lake acidification, there was a striking shift in the sediment record towards dominance by acid- and metal-tolerant species. Water quality of the fume-kill lakes has since improved (i.e. pH has increased from ~3 to ~7 and metal concentrations have decreased). In four of the five lake cores, this was reflected by a decrease in the relative abundance of acid-tolerant species. Surprisingly, diatom communities were not progressing towards pre-disturbance species assemblages. Factors impeding the return of native species might include metal enrichment in surface sediment and potentially altered lake thermal regimes. Documenting and understanding recovery trajectories is necessary to help lake managers evaluate policy decisions regarding the efficacy of emission reduction programs and mitigation measures. This thesis provides evidence from one location in addition to Sudbury that the ecological effects of severe lake acidification can be reversed if SO2 emissions are sufficiently reduced. It also further demonstrates the complexity of recovery patterns in acidified and metal-contaminated lakes.
Thesis (Master, Biology) -- Queen's University, 2009-04-26 18:38:20.58

Endocrine disruption and high occurrences of intersex have been observed in wild fish associated with wastewater treatment plant (WWTP) effluents in the urbanized reaches of the Grand River watershed located in southern Ontario, Canada. WWTP effluent is a complex matrix with diverse aquatic environmental contaminants and stressors. This study aimed to: (1) characterize the spatio-temporal distribution and fate of antiandrogenic personal care products (triclosan, chlorophene, and dichlorophene), along with selected pharmaceuticals (carbamazepine, ibuprofen, naproxen, and venlafaxine) and the herbicide, atrazine in the Grand River watershed and (2) model the behaviour of these contaminants in the aquatic environment. Water sampling of 29 sites which covered six municipal WWTPs and ~100 km of river length was completed during summer low flows (July 2012). Monthly samples were also collected immediately upstream and downstream of a major WWTP (Kitchener) from August to November 2012. Many of the target pharmaceuticals and triclosan were detected in WWTP effluents in the Grand River watershed, especially those that did not nitrify (minimal treatment with high ammonia). Chlorophene was either undetected or was only found at trace levels in the effluents. Under low flow conditions, triclosan and several other pharmaceuticals exhibited a spatial pattern where concentrations increased directly downstream of the WWTPs, then decreased with distance downstream (dilution and/or degradation). Chlorophene, in contrast, was not found downstream of most of the WWTP outfalls but was first detected at a site 5 km upstream of a WWTP and then continued with relatively constant concentrations for approximately 29 km downstream. It was also only found during the summer sampling period. Atrazine was consistently found in all sampling locations which reflected the agricultural non-point source nature of this compound. The WASP 7.5 model (US Environmental Protection Agency) was adapted and calibrated to a reach of the Grand River associated with the Kitchener WWTP. The simulation of the fate and transport of the target compounds revealed that flow-driven transport processes (advection and dispersion) greatly influence their behaviour in the aquatic environment. However, fate mechanisms such as biodegradation and photolysis also potentially play an important role in the attenuation of most compounds. The exception was carbamazepine where it was shown to act as a conservative tracer compound for wastewater specific contaminants in the water phase. The fate model developed can be applied in the future to predict the fate of a wide variety of contaminants of emerging concern across the watershed to help define the exposure of these biologically active chemicals to sensitive ecosystems.

Partial-infiltration permeable pavement (PP) systems provide environmental benefits by increasing infiltration, attenuating storm flows and improving stormwater quality. This thesis focuses on the performance and operation of partial-infiltration PP systems over low permeability soil in Ontario. Three PP, AquaPave®, Eco-Optiloc® and Hydromedia® Pervious Concrete were monitored over two years and their performance was evaluated relative to an impermeable Asphalt control. Field data was collected from the Kortright PP pilot parking lot in Vaughan, Ontario. Through the use of restrictor valves on underdrains the PP systems were shown to provide substantial hydrologic benefits by eliminating stormwater outflow for rain events less than 7mm, reducing peak flows by 91% and reducing total stormwater volume by 43%. Stormwater quality was analyzed for winter and non-winter seasons. The PP were shown to greatly reduce the concentration and total loading of suspended solids, nutrients, hydrocarbons and most heavy metals. Some water quality data, such as pH, K, or Sr levels, indicate that the quality of PP effluent will change as the system ages. Study of PP sample boxes at the University of Guelph highlighted the role that construction materials have on effluent quality and showed that pollutants introduced by the pavement and aggregate are almost entirely in a dissolved form and decline very rapidly after a season of exposure to rainfall. Benefits to water quality were sustained during winter months. The partial-infiltration PP systems were shown to provide buffering of Na and Cl concentrations. Small and large-scale maintenance practices for PP systems were investigated. Small-sized equipment testing found that vacuum cleaning and pressure-washing have good potential to improve infiltration capacity. Testing of full-sized streetsweeping trucks demonstrated that permeability can be partially restored on PICP by suction-based sweeping. Vacuum-sweeping was beneficial on a PC pavement which had experienced large permeability losses. Results of this study indicate that partial-infiltration PP systems can be effective measures for maintaining or restoring infiltration functions on parking lots and other low volume traffic areas, even in areas with low permeability soils.

The western embayment of Lake Ontario, Hamilton Harbour, is one of the most polluted sites in the Laurentian Great Lakes and in recent years has seen a reoccurrence of cyanobacterial blooms. This study uses a multidisciplinary approach to examine the presences of toxic Cyanobacteria in the harbour in order to gain insight into these recurrent blooms. Microscopic analyses of phytoplankton samples collected during the 2009 summer-fall sampling season from two locations within the harbour showed the spatial and seasonal diversity of the contemporary cyanobacterial community. Microcystis colonies relative abundances in relation to total algal numbers were estimated. The lowest and highest relative abundances of Microcystis in the phytoplankton population were 0.6% and 9.7%, respectively, and showed seasonal variability between stations. Fourteen cyanobacterial genera comprising six families and three orders were identified and for which the most abundant filamentous genera during the summer-fall sampling season were Planktothrix, Aphanizomenon and Limnothrix. Potential microcystin producers Microcystis, Planktothrix, Aphanizomenon and Dolichospermum were also present and during the sampling period Microcystis was recorded at both stations on all dates, however, its relative abundance was below 10 % throughout the study period. The composition and abundance of filamentous cyanobacteria were observed to be positively statistically correlated to water quality environmental parameters dissolved nitrates (NO3/NO2), dissolved inorganic carbon (DIC), and conductivity. Redundancy analysis (RDA) found that 53.35% total variance of Aphanizomenon was correlated to low water column NO3/NO2 and conductivity, and higher water column DIC. 58.13% of the relative abundance of Planktothrix was correlated to high concentrations of dissolved nitrates, while 51.69% of total variance of Limnothrix was correlated to higher DIC and lower water column dissolved nitrate concentrations. Information about past cyanobacterial communities was obtained from the sediment core analysis, using paleolimnological and modern molecular methods. The age of the 100.5 cm long sediment core retrieved from the deepest part of Hamilton Harbour was established to be 140 years (1869-2009), using the Constant Rate of Supply (CRS) 210Pb age model. This age was not sufficient to provide information of harbour’s environmental conditions, presence of the blooms, and triggers for their occurrence before European settlement in the area. Results of the HPLC analysis of fossil pigments indicated that the dominant members of the algal community have not changed over the 140 years and that cyanobacteria were regular members of the phytoplankton community. The composition of the major chlorophyll pigments indicated high presence of Chlorophyta and Bacillariophyta in the harbour at all times. The main algal groups identified on the basis of marker pigments presence, besides the Chlorophyta and Bacillariophyta, were the Dinophyta and the Cryptophyta. The presence of a scytonemin derivative, compound B, indicated that cyanobacterial blooms were occurring in past, before the first officially recorded blooms in the 1960s. Cyanobacterial pigments presence indicated that Cyanobacteria have been a regular but not dominant feature of Hamilton Harbour phytoplankton in the past. To our knowledge, this study is the first one examining fossil pigments from Hamilton Harbour. Results of the PCR-DGGE molecular analysis of 16S rRNA-V3 gene fragments from sedimentary DNA revealed the presence of thirteen cyanobacterial genotypes. The temporal change in the cyanobacterial community composition was indicated by the increasing number of species over time, from the oldest to the most recent sediment layers. The deepest sediment strata showed the lowest number (two bands) and intensity of bands. The most recent sediment layer had the greatest numbers (11) and intensity of bands. This increased diversity indicated changing environmental conditions in the harbour, primarily nutrient pollution and worsening water quality. Results of the PCR-DGGE molecular analysis of mcyE-AMT gene fragments showed that Microcystis aeruginosa and Planktothrix rubescens were two microcystin producers present in Hamilton Harbour over the last 80 years. The persistent presence and resilience of these two genera indicated a more serious and longer-term issue of toxic blooms than previously recognized. Historical records show that noticeable anthropogenic impact on Lake Ontario environment has been measurable since the 1780s, the first dramatic impact on the Lake Ontario watershed was evident from the mid1880s, the earliest evidence of eutrophication in the lake occurred between 1820 and 1850, while human induced environmental changes in Hamilton Harbour date back ca. 350 years. In the 1960s, cyanobacterial blooms were first officially recognized in the harbour and the lower Great Lakes. The present research is the first report of the mcyE module and AMT domain of microcystin genes being amplified from sediment of North American lakes, and showed that toxic Cyanobacterial have been regular members of Hamilton Harbour phytoplankton community for almost a century. This research considerably deepened the knowledge of the past toxic cyanobacterial blooms in Hamilton Harbour and their possible causes. It also showed that in the absence of historical records, both the PCR-DGGE method and the mcyE-AMT gene may be used for reconstruction of the past toxic blooms not only in the Laurentian Great Lakes, but also in other aquatic regions of the world impacted by toxic cyanobacterial blooms. Also, it demonstrated the utility of the combined molecular and paleolimnological analyses, which might become a useful tool in the determination of the bloom causes factors and in the mitigation of the future production of toxic blooms.