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Journal articles on the topic 'VITRO PROPAGATION'

Author: Grafiati

Published: 11 September 2023

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1

Kanwar J, K., and S. Kumar. "In vitro propagation of Gerbera: A Review." Horticultural Science 35, No. 1 (February 12, 2008): 35–44. http://dx.doi.org/10.17221/651-hortsci.

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Gerbera has gained popularity in the past few years in many countries of the world and it is in great demand in the floral industry as cut flower as well as potted plant due to its beauty, colour, long vase life, and ability to rehydrate after long transportation. The most commercial cultivars are propagated through vegetative means by multiplication through divisions of clumps; however, the multiplication by this method is too slow to be commercially viable. To commercialize this crop and to meet the growing demand for planting material, tissue and organ culture techniques are being used as alternative methods for propagation in many countries. Most of the work has been carried on plant regeneration by adventitious organogenesis from capitulum, shoot tip, leaf, petiole and other parts of the plant. Attention should be paid to improve the technology to achieve 100% success in all species/cultivars to meet growing demands of the growers globally. From the literature, it is evident that gerberas are highly amenable to in vitro studies, as various explants were found to favourably respond to different culture media with different types and concentrations of growth regulators.

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2

Sedlák, J., and F. Paprštein. "In vitro propagation of blue honeysuckle." Horticultural Science 34, No. 4 (January 7, 2008): 129–31. http://dx.doi.org/10.17221/1871-hortsci.

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We have developed a rapid shoot multiplication procedure for <I>in vitro</I> propagation of blue honeysuckle (<I>Lonicera kamtschatica</I> [Sevast.] Pojark). Shoot tips of two genotypes 20/1 and Altaj were successfully established <I>in vitro</I> and micropropagated on the Murashige and Skoog (MS) based media containing different concentrations of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the genotype and concentration of BAP. The highest multiplication rate was obtained for the genotype 20/1 that produced 10.5 ± 0.7 shoots (longer than 10 mm) on the MS medium containing 2 mg/l BAP. The lowest multiplication rate was obtained for Altaj producing only 1.6 ± 0.1 shoots on MS medium containing 4 mg/l BAP. Moreover, <I>in vitro</I> rooting on the modified MS medium supplemented with 2.5 mg/l indole-3-butyric acid (IBA) was reported. Rooted shoots were transferred to the greenhouse for further evaluation.

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3

Fotso, Tchinda Néhémie Donfagsiteli, Duclaire Mbouna, and Ndoumou Denis Omokolo. "Propagation deRicinodendronheudelotiipar bouturagein vitro." Fruits 59, no. 5 (September 2004): 351–58. http://dx.doi.org/10.1051/fruits:2004033.

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4

Debnath, Samir C. "Propagation ofVaccinium in Vitro." International Journal of Fruit Science 6, no. 2 (February 20, 2007): 47–71. http://dx.doi.org/10.1300/j492v06n02_04.

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5

Al-Aradi, Haleemah J., Ansam M. S., and Khaun A. M. Jihad M. Al-Zewar. "Propagation of water hyssop ( Bacopa monnieri L.) in vitro." IRAQI JOURNAL OF AQUACULTURE 14, no. 1 (2017): 1–12. http://dx.doi.org/10.21276/ijaq.2017.14.1.1.

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6

Chalupa, V. "In vitro propagation of European larch (Larix decidua Mill.)." Journal of Forest Science 50, No. 12 (January 11, 2012): 553–58. http://dx.doi.org/10.17221/4656-jfs.

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Methods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic embryos and cotyledons were used as initial explants. Axillary and adventitious bud cultures were used for fast in vitro shoot multiplication. Root induction was stimulated on low salt medium supplemented with auxin. After rooting and hardening off, micropropagated trees were planted in experimental plots. The growth of micropropagated trees was comparable with the height and diameter growth of trees originated from seeds.

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7

Airò, M., G. Giardina, and A. Giovino. "IN VITRO PROPAGATION OF 'FRANGIPANI'." Acta Horticulturae, no. 1083 (May 2015): 545–48. http://dx.doi.org/10.17660/actahortic.2015.1083.72.

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8

Briggs, B. A., S. M. McCulloch, and L. A. Caton. "IN VITRO PROPAGATION OF RHODODENDRON." Acta Horticulturae, no. 364 (May 1994): 21–26. http://dx.doi.org/10.17660/actahortic.1994.364.1.

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9

Vlachou, G., M. Papafotiou, and K. F. Bertsouklis. "In vitro propagation ofBallota acetabulosa." Acta Horticulturae, no. 1113 (March 2016): 171–74. http://dx.doi.org/10.17660/actahortic.2016.1113.25.

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10

Vlachou, G., M. Papafotiou, and K. F. Bertsouklis. "In vitro propagation ofCalamintha nepeta." Acta Horticulturae, no. 1113 (March 2016): 189–94. http://dx.doi.org/10.17660/actahortic.2016.1113.28.

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11

Stapfer, R. E., and C. W. Heuser. "In Vitro Propagation of Periwinkle." HortScience 20, no. 1 (February 1985): 141–42. http://dx.doi.org/10.21273/hortsci.20.1.141.

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Abstract Single node explants from in vitro derived shoots of Vinca minor L. ‘Bowlesii’ were cultured on modified woody plant medium (WPM). A more effective promoter of axillary shoot proliferation was 6-benzylamino purine (BA) than 6-furfurylamino purine (kinetin) or 6-(γ,γ-dimethylallylamino) purine (2iP). Maximum production of shoots 5 mm and longer occurred in a medium containing 64 μm BA and 0.1 μm α naphthaleneacetic acid (NAA), whereas 64 μm BA and 1.0 μm NAA produced the most shoots 10 mm and longer. Proliferated shoots were rooted and established in a soilless medium under high humidity.

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12

Janick, Jules, James E. Simon, and Anna Whipkey. "In Vitro Propagation of Borage." HortScience 22, no. 3 (June 1987): 493–95. http://dx.doi.org/10.21273/hortsci.22.3.493.

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Abstract In vitro shoot proliferation of borage (Borago officinalis L.) was achieved in a basal medium based on Murashige and Skoog salts supplemented with 17.6 μm BA (4 mg·liter−1) plus 10% (v/v) coconut water. Rooting of in vitro-produced shoots occurred in basal media and increased in response to the addition of IBA. Rooted shoots were transferred successfully to soil. Immature zygotic embryos cultured in 4.5 μm 2,4-D (1 mg·liter−1) plus coconut water (CW) produce asexual embryos directly from the cotyledonary surface and indirectly from callus. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy)acetic acid (2,4-D); 1H-indole-3-butanoic acid (IBA).

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13

Hosier, Mary A., Gro Flatebo, and Paul E. Read. "In Vitro Propagation of Lingonberry." HortScience 20, no. 3 (June 1985): 364–65. http://dx.doi.org/10.21273/hortsci.20.3.364.

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Abstract Shoot proliferation of lingonberry (Vaccinium vitis-idaea ssp. minus) was achieved in vitro from shoot tips excised from 1-year-old stock plants and cultured on a low-salt medium supplemented with 2iP. Rooting was obtained without auxin application by placing microshoots in a peat-vermiculite medium in a high humidity chamber. After 6 weeks, the rooted plantlets were transplanted into a peat-lite mix, subjected to a 2-week low humidity acclimation process and subsequently acclimated in a greenhouse under 50% shade. Within 9 months, these plantlets developed a rhizomatous growth habit, in contrast to the nonrhizomatous habit of conventionally rooted cuttings. Chemical names used: N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP).

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14

Economou, Athanasios S., and Maria J. Spanoudaki. "In Vitro Propagation of Gardenia." HortScience 20, no. 2 (April 1985): 213. http://dx.doi.org/10.21273/hortsci.20.2.213.

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Abstract Shoot proliferation of Gardenia jasminoides Ellis was achieved from cultured shoot tips on a low salt medium supplemented with 25–98 μm (5–20 mg/liter) 2iP after 10 weeks. Reculture of the remaining tissue onto the same medium, after shoot removal, resulted in new shoot proliferation in 6 weeks. These microshoots rooted readily when excised and inserted into sphagnum moss peat under high humidity. Chemical name used. 2iP: N-(3-methyl-2-butenyl)-lH-purin-6-amine.

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15

Calvo, Maria Carmen, and Juan Segura. "In Vitro Propagation of Lavender." HortScience 24, no. 2 (April 1989): 375–76. http://dx.doi.org/10.21273/hortsci.24.2.375.

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Abstract Cultures were established for micropropagation of Lavandula latifolia Medicus from hypocotyl explants. Bud induction was achieved by placing the explants on Murashige and Skoog (MS) medium supplemented with 0.60 μM IAA and 8.9 μM BA. The buds developed into numerous shoots on transfer to several elongation media. Shoots were easily rooted (95%) on MS hormone-free medium with macronutrients at half-strength. Plantlets were successfully transplanted into soil. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); 1H-indole-3-acetic acid (IAA).

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16

Kancherla, S. Latha, and Prem L. Bhalla. "In Vitro Propagation of Pandoreas." HortScience 36, no. 2 (April 2001): 348–50. http://dx.doi.org/10.21273/hortsci.36.2.348.

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Pandoreas, Australian natives of horticultural significance, were successfully propagated using tissue culture. A protocol for rapid in vitro multiplication of commercial cultivars was developed using nodal segments cultured on Murashige and Skoog medium containing either BA or kinetin. Maximum shoot induction and number of shoots per explant for P. pandorana (Andrews) Steenis and P. jasminoides (Lindley) Schumann were on 8.8 μm BA and 4.6 μm kinetin. Higher levels of cytokinin in the medium inhibited shoot formation. Tissue-cultured shoots were rooted with IBA. This study demonstrates that Pandoreas can be successfully micropropagated. Chemical names used: 6-benzylaminopurine (BA); 3-indole butyric acid (IBA).

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17

Kuijpers, A. M., and M. Langens-Gerrits. "PROPAGATION OF TULIP IN VITRO." Acta Horticulturae, no. 430 (December 1997): 321–24. http://dx.doi.org/10.17660/actahortic.1997.430.49.

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18

Journal, Baghdad Science. "Propagation of Chickpea in vitro." Baghdad Science Journal 7, no. 4 (December 5, 2010): 1322–30. http://dx.doi.org/10.21123/bsj.7.4.1322-1330.

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Apical meristems, lateral buds, anthers of immature flowers and immature embryos of chickpea ( Cicer arietinum L.) were cultured on MS media with different growth regulators and incubated for 6 weeks at 25-27?C with 16 hrs photoperiod for callus initiation. Results indicated that 1 and 0.1 mg/l of 2,4-D and BA were suitable for callus initiation when apical meristems and lateral buds were used. While 2 and 0.5 mg/l of both growth regulators were essential for immature embryos. It was noticed that using chickpea anthers of the MS medium must contain 1mg/l 2ip and 0.5 mg/l IAA. However, MS medium supplemented with 1-3 mg/l of BA and 2,4-D respectively was good for callus initiation from lateral buds, anther and immature embryos. However, callus differentiations in chickpea were successfully obtained when 2-3 mg/l of IAA, 2-2.5mg/l of kinetin or 0.1 mg/l of NAA and 2 mg/l of kinetin were used. Data of regeneration and culture maintenance revealed that half strength of MS medium supplemented with 2, 2.5 mg/l of IAA and kinetin respectively or 0.005mg/l and 0.05 mg/l of NAA and BA respectively was the best. The importance of this method in propagation were used for improving and screening resistant chickpea germplasm aginst Fusarium wilt disease.

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19

Ma, Yan, David H. Byrne, and Jing Chen. "Propagation of rose speciesin vitro." In Vitro Cellular & Developmental Biology - Plant 32, no. 2 (April 1996): 103–8. http://dx.doi.org/10.1007/bf02823139.

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20

Purohit, S. D., and G. Kukda. "In vitro propagation ofWrightia tinctoria." Biologia plantarum 36, no. 4 (December 1, 1994): 519–26. http://dx.doi.org/10.1007/bf02921172.

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21

Ndoumou, Denis Omokolo, Fotso, Oumar, and Duclaire Mbouna. "Propagation d'Irvingia gabonensispar microbouturagein vitro." Fruits 59, no. 1 (January 2004): 31–38. http://dx.doi.org/10.1051/fruits:2004004.

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22

Das, Sudripta, Timir B. Jha, and Sumita Jha. "In vitro propagation of cashewnut." Plant Cell Reports 15, no. 8 (April 1996): 615–19. http://dx.doi.org/10.1007/bf00232464.

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23

Jha, Timir B., Sumita Jha, and Sudripta Das. "In vitro propagation of cashewnut." Plant Cell Reports 15, no. 8 (April 1, 1996): 615–19. http://dx.doi.org/10.1007/s002990050085.

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24

Kaliamoorthy, Seventhilingam, Harsh Chowdhery, and G. Murthy. "In vitro propagation of orchids." Indian Journal of Forestry 28, no. 4 (December 1, 2005): 439–50. http://dx.doi.org/10.54207/bsmps1000-2005-4s8784.

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25

Nekrasov, E. V. "IN VITRO PROPAGATION OF ARMENIACA MANDSHURICA (ROSACEAE)." Бюллетень Ботанического сада ДВО РАН, no. 18 (December 25, 2017): 81–88. http://dx.doi.org/10.17581/bbgi1814.

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26

Lekonceva, Tat'yana, and Aleksandr Fedorov. "Improvement of the grapes propagation technology in vitro." Agrarian Bulletin of the 200, no. 9 (September 30, 2020): 55–62. http://dx.doi.org/10.32417/1997-4868-2020-200-9-55-62.

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Abstract. The aim of research is improvement of the production technology in vitro of the grapes Pamyati Dombkovskoy. Methods. Methods generally accepted in the practice of clonal micropropagation of plants were applied. There are sterilization of the starting material, introduction into culture, clonal micropropagation, and in vitro rooting, followed by adaptation to in vivo conditions. The study object was micro cuttings grapes of the cultural variety Pamyati Dombkovskoy. The experiments were set up in three replicates, one replication was at least 10 test tubes. Statistical processing of the data obtained was carried out by the dispersion method according to B. A. Dospekhov. The following parameters were taken into account: micro-shoots and microplants heights, leaves number, proliferation coefficient. Root development was assessed in points. The success of adaptation was considered as the percentage of adapted microplants to the total number planted in the substrate. At the stage of adaptation, we applied supplemented and developed by us technique during clonal micropropagation of the Angelica rose. Research result. Success of estanlishment explants was 40 % on Murashige and Skoog growing medium with a reduced content of macronutrients, when introduced into a sterile culture in vitro. The optimal concentration of 6-benzylaminopurine (6-BAP) was determined at the proliferation stage – 1 mg/l. There are decrease in the proliferation coefficient from 4.4 pcs/cutting to 3.3 and 2.9 pcs/cutting respectively with an increase in the concentration of cytokinin 6-BAP to 2.0 and 3.0 mg / l (LSD05 = 1.0). It was revealed that the best growing medium for rooting micrograpes is the environment according to Zlenko and others, when the best microplants development is achieved according to such morphometric parameters as microshoots height, leaves number and root system. On the medium Zlenko and others, microplants height was higher by 4.3 mm than the control with LSD05 = 2.7, the number of leaves was more by 0.5 with LSD05 = 0.3, and the root system of microplants is better developed by 0.4 points (LSD05 = 0.2). Scientific novelty. Positive results were received when rooted grapes cuttings were adapted after 14 days of cultivation on rooting medium, which allows to reduce the duration of micro grapes in a test tube in 2–4 times compared with the conventional method. The use of the biological product “Trichoderma Veride” for watering the soil substrate with the subsequent spraying of adaptable microplants with the organosilicone fertilizer “Siliplant” is recommended.

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27

Templeton-Somers, Karen M., and Wanda W. Collins. "Field Performance and Clonal Variability in Sweet Potatoes Propagated in Vitro." Journal of the American Society for Horticultural Science 111, no. 5 (September 1986): 689–94. http://dx.doi.org/10.21273/jashs.111.5.689.

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Abstract Six methods of propagating sweet potato (Ipomoea batatas L.), including 3 in vitro propagation methods, nodal propagation, and propagation by slips and cuttings from bedded roots of ‘Jewel’, were evaluated for field performance of the resulting plants and for the degree of plant-to-plant variability. The 2-year experiment (1982 and 1983) included a carryover study with plants obtained from the bedded roots of the first year's study. Plants were evaluated individually for yield, skin and flesh color, mutation frequency, dry matter, and protein content. In both years, plants propagated by stem cuttings and by slips from bedded roots produced higher yields than those propagated by tissue culture methods. Plants propagated by regeneration from cultured leaf explants were consistently lower in yield than plants obtained by all other methods. In the 1983 study, plants from stem cuttings from field-bedded roots significantly out-yielded plants from all other treatments. Stem cuttings from roots bedded in pots in a growth chamber out-yielded all tissue culture propagation methods, even though the tissue-culture explants also were obtained from these same roots. The carryover study showed that all differences in yield due to propagation method were dissipated with a single cycle of the normal propagation method for sweet potato. When yield data were analyzed according to the mother root origin of the plant material, there were significant differences among plants originating from different roots. Differences in skin color mutation frequencies due to propagation method existed in the 1982 study; however, the 1983 study demonstrated that these differences were also due to the origin of the plant material.

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28

KARPUSHINA, M. V., and M. A. VINTER. "PROPAGATION OF GARDEN STRAWBERRIES IN CULTURE IN VITRO." Scientific Works of North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making 33 (July 2021): 9–14. http://dx.doi.org/10.30679/2587-9847-2021-33-9-14.

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29

Gawel, N. J., C. D. Robacker, and W. L. Corley. "In Vitro Propagation of Miscanthus sinensis." HortScience 25, no. 10 (October 1990): 1291–93. http://dx.doi.org/10.21273/hortsci.25.10.1291.

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Immature inflorescences of Miscanthus sinensis Andress. `Gracillimus', `Variegatus', and `Zebrinus' were cultured on modified MS medium with 9.0 μm 2,4-D, 20 g sucrose/liter, 2.0 g Gelrite/liter, and 0.75 g MgCl2/liter. Organogenesis was observed 8 to 12 weeks after callus initiation. Shoots were rooted on half-strength MS medium without growth regulators. After rooting, tillers were initiated. When transferred to soil, plants matured to flowering quickly and retained their variegation patterns. Propagation through in vitro tillering is suggested. Chemical name used: 2,4-dichlorophenoxyacetic acid (2,4-D).

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30

Wickremesinhe, E. R. M., W. J. Blackmon, and B. D. Reynolds. "In Vitro Propagation of Apios americana." HortScience 25, no. 11 (November 1990): 1439–40. http://dx.doi.org/10.21273/hortsci.25.11.1439.

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Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

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31

Horshati, E., and E. Jambor-Benczur. "IN VITRO PROPAGATION OF CAPPARIS SPINOSA." Acta Horticulturae, no. 725 (November 2006): 151–54. http://dx.doi.org/10.17660/actahortic.2006.725.14.

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32

Elhag, Hamid M. "In Vitro Propagation of Catha edulis." HortScience 26, no. 2 (February 1991): 212. http://dx.doi.org/10.21273/hortsci.26.2.212.

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33

Smith, R. H., P. J. Burdick, J. Anthony, and A. A. Reilley. "In Vitro Propagation of Coryphantha macromeris." HortScience 26, no. 3 (March 1991): 315. http://dx.doi.org/10.21273/hortsci.26.3.315.

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34

Upfold, S. J., J. van Staden, and T. J. Edwards. "In Vitro Propagation of Rhodohypoxis baurii." HortScience 27, no. 11 (November 1992): 1230. http://dx.doi.org/10.21273/hortsci.27.11.1230.

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35

Mezzetti, B., G. Minotta, O. Navacchi, and P. Rosati. "IN VITRO PROPAGATION OF ULMUS CARPINIFOLIA." Acta Horticulturae, no. 227 (September 1988): 396–98. http://dx.doi.org/10.17660/actahortic.1988.227.72.

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36

INAMOTO, Yasumi, and Yoshiaki KITANI. "In vitro propagation of Cinnamomum cassia." Plant tissue culture letters 6, no. 1 (1989): 25–27. http://dx.doi.org/10.5511/plantbiotechnology1984.6.25.

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37

Gonçalves, A. ""IN VITRO" PROPAGATION OF PALM TREES." Acta Horticulturae, no. 360 (August 1994): 149–56. http://dx.doi.org/10.17660/actahortic.1994.360.19.

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38

Sotthikul, C., C. Kaewpoowat, and N. Saimoon. "In vitro propagation of Habenaria hybrids." Acta Horticulturae, no. 1155 (March 2017): 293–300. http://dx.doi.org/10.17660/actahortic.2017.1155.42.

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39

Papafotiou, M., N. Tsalouchos, A. Papagianni, and K. F. Bertsouklis. "In vitro propagation of Lippia citriodora." Acta Horticulturae, no. 1155 (March 2017): 327–30. http://dx.doi.org/10.17660/actahortic.2017.1155.47.

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40

Soomro, Rashida, Shamsa Yasmin ., and Rizwana Aleem . "In vitro Propagation of Rosa indica." Pakistan Journal of Biological Sciences 6, no. 9 (April 15, 2003): 826–30. http://dx.doi.org/10.3923/pjbs.2003.826.830.

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41

Minocha, Subhash C. "In Vitro Propagation of Dionaea muscipula." HortScience 20, no. 2 (April 1985): 216–17. http://dx.doi.org/10.21273/hortsci.20.2.216.

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Abstract Surface-sterilized leaf sections from mature Venus fly trap (Dionaea muscipula Ellis) plants produced several adventitious plantlets when cultured on full strength Lin and Staba medium or a reduced strength Murashige and Skoog growth medium supplemented with 0.5-25 μM cytokinin. Zeatin at 9.0 or 22.5 μm was found to be most effective in inducing adventitious shoot formation. These shoots were excised and subcultured on a low salt medium to generate roots. The rooted plants then were transferred to sterilized peat moss and grown to maturity. A large number of plants could be regenerated through repeated subculture of leaf segments.

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42

Cockrel, Angela D., G. L. McDaniel, and E. T. Graham. "In Vitro Propagation of Florists’ Cineraria." HortScience 21, no. 1 (February 1986): 139–40. http://dx.doi.org/10.21273/hortsci.21.1.139.

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Abstract Tissue culture propagation of Senecio cruentus DC. ‘Hansa’ was achieved from shoot tips derived from selected seedlings germinated in vitro. Shoot multiplication was best accomplished on Murashige and Skoog (MS) gerbera medium supplemented with 2.8 μM IAA. Maximum root formation occurred on MS modified medium with reduced salt levels and 1.3 μM NAA. Rooted shoots were established successfully in a soilless medium under high humidity. Chemical names used: 1H-indole-3-acetic aid (IAA); 1-naphthaleneacetic acid (NAA).

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43

Zhang, Baolin, L. P. Stoltz, and J. C. Snyder. "In Vitro Propagation of Euphorbia fulgens." HortScience 22, no. 3 (June 1987): 486–88. http://dx.doi.org/10.21273/hortsci.22.3.486.

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Abstract Nodal sections of actively growing apical shoots from greenhouse-grown plants of Euphorbia fulgens Karw. ex Klotsch initiated new shoots after 4 weeks on a modified Murashige and Skoog (MS) medium supplemented with 9.1 μm zeatin. When cultures from the initiation stage were transferred for proliferation to the same medium, up to 14 shoots 5 mm long or longer were obtained per culture 4 weeks later. Through subcultures, 40 transplantable shoots per explant could be produced within 12 weeks. Shoots were rooted in vitro in the greenhouse with satisfactory survival rates. Chemical names used: (E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin).

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44

Bailey, Douglas A., Gary R. Seckinger, and P. Allen Hammer. "In Vitro Propagation of Florists’ Hydrangea." HortScience 21, no. 3 (June 1986): 525–26. http://dx.doi.org/10.21273/hortsci.21.3.525.

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Abstract The effects of repetitive subculturing, cytokinin species and concentration, basal medium, and gelatinizing constituent were studied to maximize shoot multiplication of Hydrangea macrophylla Thunb. ‘Rose Supreme’ shoot tip cultures. Cytokinin treatments of BA or 2iP at 2, 4, 8, 16, or 32 μm were incorporated into woody plant medium (WPM) solidified with 6 g·liter−1 Sigma agar. Cultures grown on 8 μm BA produced the greatest number of useable shoots per 4-week subculture. No treatment stimulated shoot multiplication until the 2nd subculture, after which shoot multiplication remained constant within treatments through the 5th subculture. Results indicate no difference in number of shoots produced per subculture on modified Gamborg's B5, modified Murashige and Skoog basal medium, or WPM when incorporating 8 μm BA and 6 g·liter−1 Sigma agar. No difference in shoot multiplication was observed between 6 g·liter−1 Sigma agar and 2 g·liter−1 Gel-Rite when incorporated into WPM containing 8 μm BA. However, culture fresh weight and shoot length was greater for cultures on Gel-Rite than for cultures on agar. Chemical names used: N-(phenylmethyl)-lH-purin-6-amine (BA) and N-(3-methyl-2-butenyl)-lH-purin-6-amine (2iP).

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45

Moore, G. A. "In Vitro Propagation of Citrus Rootstocks." HortScience 21, no. 2 (April 1986): 300–301. http://dx.doi.org/10.21273/hortsci.21.2.300.

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Abstract Internodal seedling stem sections of 3 Citrus rootstocks—sour orange (C. aurantium L.), ‘Carrizo’ citrange [C. sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.], and ‘Cleopatra’ mandarin (C. reshni Hort. ex Tanaka)—were cultured on media containing varying concentrations of BA and NAA. A medium containing 22 μM BA with or without 5.4 μM NAA was optimum for shoot initiation in all 3 rootstock types, but the 3 genotypes varied greatly in numbers of shoots produced. NAA in the medium was inhibitory to shoot production in long-term cultures. Chemical names used: N- (phenylmethyl)-1H-purin-6-amine (BA) and 1-naphthaleneacetic acid (NAA).

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46

Janick, Jules, and Anna Whipkey. "In Vitro Propagation of Cuphea wrightii." HortScience 21, no. 1 (February 1986): 135–37. http://dx.doi.org/10.21273/hortsci.21.1.135.

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Abstract In vitro propagation of Cuphea wrightii Gray was achieved by culturing leaves or shoots in a basal medium based on Murashige and Skoog salts. The cytokinin BA induced callus from leaves, shoots, hypocotyls, and epicotyls that was capable of initiating shoots as well as axillary branching, but callus induced from seedling roots did not differentiate shoots. Proliferated shoots transferred to a basal medium without BA produced roots and were successfully transferred to soil. Freshly harvested seed failed to germinate, but 31% of excised embryos developed when cultured on basal medium, and 63% developed in response to basal medium supplemented with 4.4 μM (lmg/liter) BA. Chemical names used: N-(phenlmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy)acetic acid (2,4-D); and 1H-indole-3-butanoic acid (IBA).

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47

van Aartrijk, J., and P. C. C. van der Linde. "IN VITRO PROPAGATION OF BULBOUS CROPS." Acta Horticulturae, no. 177 (December 1986): 278. http://dx.doi.org/10.17660/actahortic.1986.177.39.

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48

Devi, C. Subathra, and V. Mohana Srinivasan. "In vitro Propagation of Gymnema sylvestre." Asian Journal of Plant Sciences 7, no. 7 (September 15, 2008): 660–65. http://dx.doi.org/10.3923/ajps.2008.660.665.

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Oliveira, João Antônio Ramos de, Diego Pascoal Golle, André Schoffel, Juliane Nicolodi Camera, and Jana Koefender. "Physalis angulata L. propagation in vitro." Revista Ceres 66, no. 6 (December 2019): 486–92. http://dx.doi.org/10.1590/0034-737x201966060010.

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Hoepfner, A. "IN VITRO PROPAGATION OF RED RASPBERRY." Acta Horticulturae, no. 262 (November 1989): 285–88. http://dx.doi.org/10.17660/actahortic.1989.262.41.

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