Dissertations / Theses on the topic 'Vitamin B6'
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KUZUYA, FUMIO. "Vitamin B6 and Arteriosclerosis." Nagoya University School of Medicine, 1993. http://hdl.handle.net/2237/17526.
Full textTambasco, Studart Marina. "Analysis of vitamin B6 biosynthesis in Arabidopsis thaliana /." Zürich : ETH, 2007. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17120.
Full textStudart, Marina Tambasco. "Analysis of vitamin B6 biosynthesis in Arabidopsis thaliana." kostenfrei, 2007. http://e-collection.ethbib.ethz.ch/view/eth:29544.
Full textGandhi, Amit. "VITAMIN B6 METABOLISM AND REGULATION OF PYRIDOXAL KINASE." VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/2008.
Full textDesai, Jigarkumar. "Pyridoxal Kinase: Its Role in Vitamin B6 Metabolism." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/2254.
Full textVan, der Westhuizen Christian Abraham. "Kinetic studies of vitamin B6 metabolism in humans." Diss., University of Pretoria, 2001. http://hdl.handle.net/2263/22786.
Full textDissertation (MSc (Chemical Pathology))--University of Pretoria, 2006.
Chemical Pathology
unrestricted
Speitling, Annette. "Wirkungen akut und chronisch hochdosierter Vitamin-B6-Gaben : biokinetische Untersuchungen am Menschen /." Giessen : Wiss. Fachverl, 1991. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=003444484&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Full textConsiglieri, Vladi Olga. "Doseamento da vitamina B6 por espectrofotometria derivada no ultravioleta." Universidade de São Paulo, 1992. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-10072008-170738/.
Full textA rapid and selecrive method for rhe dererminarion of pyridoxine in pharmaceuticals has been described. The procedure has been developed using direct UV first-derivative spectrofotometry in solid and liquid preparations (tablets, oral solution and injection). Spectral inrerferences from formulation excipienrs and other drugs in simple UV spectrophotometric methods have been eliminated by the application of the proposed method. Calibration curves have been made and the correlation coefficienr for. the first-order derivative was 0,99997. Standard deviation, coefficient of variation and confidence interval were calculated. The method was applied in the analysis of commercial and simulated samples. The results when compared with those obtained by using the USP 22nd. ed. official method shows clear advanrages related to accuracy, precision and practical application.
Pittaya, Kanchanapakonchai Amnuay Thithapandha. "Effects of vitamin B6 on CC14 toxicities in rats /." abstract, 1986. http://mulinet3.li.mahidol.ac.th/thesis/2529/29E-Pittaya-K.pdf.
Full textFootitt, E. J. "Vitamin B6 and serotonin metabolism in neurological disorders of childhood." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1413008/.
Full textMayengbam, Shyamchand S. "Characterization, quantification, and in vivo effects of vitamin B6 antagonists from flaxseed on amino acid metabolism in a rodent model of moderate vitamin B6 deficiency." ACS Publications, 2014. http://hdl.handle.net/1993/30741.
Full textOctober 2015
Almeida, Lana Carneiro. "Preditores dietéticos das concentrações séricas ou plasmáticas de homocisteína, ácido fólico, vitaminas B12 e B6 em mulheres." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/6/6133/tde-25062007-175008/.
Full textObjective To examine whether measurements of dietary intakes, obtained with a validated quantitative food frequency questionnaire (FFQ), correlated with serum or plasma levels of folic acid, vitamins B12 and B6 and homocystein (hcy) measured in low-income women living in São Paulo, Brazil. Population and methods We analyzed dietary data from 1434 women aged 21-65 years enrolled in a case-control study of diet and cervical cancer carried out in three public hospitals of São Paulo. Data for women with invasive cervical cancer were excluded. All participants had their usual dietary intake assessed by interview, using a validated FFQ, and provided a fasting blood sample for serum and plasma separation. Serum concentrations of folic acid and vitamin B12 were measured by fluorimmunoassay, while serum levels of vitamin B6 and plasma levels of hcy were measured by reversed-phase high performance liquid chromatography. Correlations between estimates of food and energy-adjusted nutrient intakes and levels of folic acid, vitamins B12 and B6 and hcy were assessed using multiple linear regression models, adjusted for covariates such as age, body mass index, lifestyle (including smoking), past and current gynecologic morbidity and obstetric history, and use of oral contraceptives. Results Although only 6.2% of the study participants had serum folic acid levels below the reference value of 7 nmol/L, 45.7% and 97.1% had a dietary intake of folic acid estimated to be less than 180 g/day and 400 g/day, respectively. Multiple linear models showed serum folic acid levels to be positively correlated with the estimated intake of protein, iron, folate, vitamins B1, B3, B6, A and C, citrus fruits and juices and green vegetables, and negatively correlated with the estimated intake of fat, sweets and dairy products. Similar results were obtained after a further adjustment for fiber intake in the model, except for the estimated intake of folic acid and green vegetables, which lost their statistical significance as independent predictors of serum folic acid levels. Serum levels of vitamin B12 below the cut-off point of 148 pmol/L were found in 11.0% of study participants; most of them (70.4%) had their vitamin B12 intake estimated to be equal or greater than the reference value of 2 g/day. Serum levels of vitamin B12 were positively correlated with the estimated intake of dairy products and vitamins B2 and B12. The estimated intakes of fiber, vitamin E and beans were negatively correlated with serum levels of vitamin B12. Dietary vitamin B6 was estimated to be below the recommended levels of 1.3 mg/day (age 50 years) or 1.5 mg/day (age > 50 years) in 49.0% of study participants. No correlation was found between estimated intakes of foods and nutrients and plasma levels of vitamin B6. Hcy concentrations were positively correlated with the estimated intake of carbohydrates and sweets, and negatively correlated with the estimated intake of protein, cholesterol, iron, zinc of animal origin, vitamins A, B2, B12 and B6 and fishes. However, these correlations were no longer significant after additional adjustment for dietary protein, the strongest predictor of hcy plasma levels. Conclusion The estimated dietary intakes of selected foods and nutrients were shown to be independent predictors of measured serum levels of folic acid and vitamin B12, providing a basis for indentifying the main dietary sources of these nutrients in this and similar populations. The strong negative correlation between plasma levels of hcy and dietary protein provides a basis for future nutritional interventions. No correlation was found between plasma concentrations of vitamin B6 and estimated dietary intakes.
Porter, Charmaine L. Locy Robert D. "Functional identification and characterization of an Arabidopsis thaliana gene involved in vitamin B6 biosynthesis." Auburn, Ala., 2005. http://hdl.handle.net/10415/1261.
Full textDunn, Karen A. "The relationship between vitamin B6 and the degree of PMS symptomatology /." Staten Island, N.Y. : [s.n.], 1990. http://library.wagner.edu/theses/nursing/1990/thesis_nur_1990_dunn_relat.pdf.
Full textPresoto, Ana Elisa Ferreira. "Vitaminas do complexo B e ferro em farinhas de cereais." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-21112017-145753/.
Full textComplex B vitamins and iron are present in some cereal foods, a kind of food largely consumed by Brazilian people. The total of these micronutrients can be significant at the dairy ingestion portion and in the consume of industrialized products enriched with complex B vitamins and iron contribute in the recommended dietary intake of these micronutrients. Table of food composition do not report complete data of complex B vitamins and iron in cereal flours. In Brazil, since 2002 the addition of folic acid and iron in com and wheat flours is compulsory. Therefore, to adapt the label of some products enriched with vitamins and iron, there is necessary the development and validation of analytical methods. These methods must be reliable and with enough sensitivity to analyse complex B vitamins and iron, in low concentration, wich are natural content in food. The purpose of this work is the evaluation, with validated methods, of the content of seven complex B vitamíns (B1, B2, B6, niacin, folic acid, pantothenic acid and biotin) and iron in five kinds of cereal flours (oat, rice, barley, com and wheat). These raw materiais are used in food industry in order to enrich the industrialized products.
Andrade, Jirrah Pedro de. "Desenvolvimento e eficácia clínica de dermocosméticos para a pele acneica contendo vitamina B3 e derivados de vitamina B6 e zinco." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/60/60137/tde-30012014-081127/.
Full textAcne is a skin disease with high prevalence and its treatment is important to prevent permanent skin lesions or the aggravation of psychological disorders due to self-esteem shaken. This way, the development of effective dermocosmetic formulations, that can improve the conditions of this skin type, is very important. Vitamin B3, a vitamin B6 derivative and zinc PCA are among the active ingredients which present potential benefits in the controlling of some pathogenic factors of acne. Thus, the aim of this research was to develop cosmetic formulations for acneic skin containing vitamin B3, vitamin B6 lipophilic derivative and zinc PCA, as well as the evaluation of stability and clinical efficacy. For this purpose, were developed different formulations which, at first, were evaluated in terms of stability face to zinc PCA addition. After this, the others active ingredients were added and the formulations were submitted to preliminary tests of stability and physical stability studies by rheological behavior determination. The most stable formulation was subject to skin compatibility evaluation and vehicle comedogenicity. The formulation with the active ingredients was also evaluated regarding their sensorial characteristics and clinical efficacy. Efficacy studies were performed by means of objective and subjective methods, after a sixweek- period of use of the formulation. The objective methods consisted in non-invasive in vivo methodologies (biophysical techniques and image analysis) where were evaluated hydration, barrier function, lipid content, skin pH and the counting of porphyrins, microcomedones and inflammatories lesions. In relation to subjective methods, was performed the efficacy perception using a questionnaire in order to compare the skin before and after the treatment. The results showed that among the formulations developed, only one kept stable after the stability tests. The formulations were considered as \"very good\" on skin compatibility test and showed no comedogenic potential. In sensorial evaluation, frequencies obtained for the parameters considered bad were low, which indicate the sensorial of the formulation was adequate for the purposes. In clinical efficacy study, the formulation under study did not alter the parameters related to hydration and skin barrier function and was effective in reducing the counting of porphyrins and inflammatories lesions (p<0,05). Clinical evaluation by subjective methods showed the formulation effectiveness regarding the improvement of inflammatory acne, skin oiliness, hydration and softness. Finally, the results obtained showed the formulation developed is effective and compatible with the skin and, besides the importance of research and development for obtaining stable, safe and effective formulations with suitable sensorial.
Ho, Chia-ling. "Vitamin B6 status of young and older adult women in Metro Vancouver." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62700.
Full textSousa, Bruno Soares de. "Investigação do potencial ansiolítico de Magnésio e Vitamina B6 em uma única administração em humanos." Universidade Federal da Paraíba, 2013. http://tede.biblioteca.ufpb.br:8080/handle/tede/4295.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The objective this study was to investigate the potential anxiolytic Magnesium and Vitamin B6 in college students using a model of experimental induction of anxiety, through the assessment of physiological and psychological parameters. The study was an experimental, randomized and controlled. It was composed of sixty students, female, distributed in one of four experimental groups: Control; Magnesium, Magnesium + B6, Vitamin B6. The human anxiety was induced by Simulated Public Speaking (TSFP) and was assessed by physiological parameters (Systemic Blood Pressure, Heart Rate, Temperature of Extremity , Electrical Skin Conductance) and psychological parameters (State - Trait Anxiety Inventory). The behavior of physiological and psychological measures was accompanied on the four times of testing, Baseline (B), Pre - stress (PT), Performance (S) and Final (F) and were evaluated in two ways: behavior between the supplemented groups and between the phases in each respective time groups. During speech, the diastolic blood pressure was lower in B6 versus control (P <.05), there was also a decrease in the conductance B6 group (P <0.01) in the Magnesium + B6 group (P <0.05) and group B6 (P <0.05) compared to the control group. In the final moment, the conductance was lower in B6 compared with controls (P <0.05). The STAI-T showed that the university had moderate levels of anxiety (STAI-T 40-60 points). In the comparison between groups was observed that all individuals of the respective groups already started the test with a moderate degree of anxiety (STAI-E 40 - 60 points). At the last moment there was a decrease in E-STAI score in both groups, where they began to be classified with low levels of anxiety (STAI-E <40 points) (P> 0.05). The use of magnesium and vitamin B6, at a concentration of 200 mg enough results showed that proves its efficacy in controlling some symptoms of anxiety induced experimentally here, exhibited lower values for CEP and anticipatory anxiety having vitamin B6 submitted lower values of DBP at the performance of the speech, which means to say that in the control group participants showed less variability.
O objetivo deste estudo foi investigar o potencial ansiolítico do Magnésio e da Vitamina B6 em estudantes universitárias utilizando um modelo de indução experimental de ansiedade, por meio da avaliação dos parâmetros fisiológicos e psicológicos. O estudo teve caráter experimental, randomizado e controlado. Foi composto por sessenta estudantes, do sexo feminino, distribuídas em um dos quatro grupos experimentais: Controle; Magnésio; Magnésio + B6; Vitamina B6. A ansiedade humana experimental foi induzida pelo Teste de Simulação de Falar em Público (TSFP) e foi avaliada por meio de parâmetros fisiológicos (Pressão Arterial Sistêmica, Frequência Cardíaca, Temperatura de Extremidades, Condutância Elétrica da Pele) e de parâmetros psicológicos (Inventário de ansiedade Traço e Estado). O comportamento das medidas fisiológicas e psicológicas foi acompanhado nos quatro momentos do teste, Basal (B), Pré - estresse (PT), Performance (S) e Final (F) e foram avaliados de duas formas: comportamento entre os grupos suplementados e entre as fases em cada um dos respectivos grupos.No momento do discurso a pressão arterial diastólica foi menor no grupo B6 comparado ao controle (P<0,05), houve ainda diminuição da condutância no grupo B6 (P<0,01), no grupo Magnésio + B6 (P<0,05) e no grupo B6 (P<0,05) comparados ao grupo controle. No momento final a condutância foi menor no grupo B6 comparado ao controle (P<0,05). O IDATE-T demonstrou que as universitárias apresentavam níveis de ansiedade moderado (IDATE-T 40-60 pontos). Na comparação entre os grupos observou-se que todos os indivíduos dos respectivos grupos já iniciavam o teste com um grau de ansiedade moderada (IDATE-E 40 - 60 pontos). No momento final houve diminuição no escore do IDATE-E em ambos os grupos, onde os mesmos passaram a ser classificados com grau de ansiedade baixa (IDATE-E < 40 pontos) (P>0,05). A utilização de Magnésio e de vitamina B6, na concentração de 200 mg apresentou resultados suficientes que comprovam sua eficácia no controle de alguns sintomas da ansiedade, aqui induzida de forma experimental, apresentando menores valores de CEP durante a ansiedade antecipatória e tendo a vitamina B6 apresentado valores menores da PAD no momento da performance do discurso, o que significa afirmar que em relação ao grupo controle as participantes apresentaram menor variabilidade.
Coutinho, Leonam Gomes. "Estudo do papel da prote?na multifuncional APE1/Ref-1 sobre a resposta inflamat?ria na meningite bacteriana." Universidade Federal do Rio Grande do Norte, 2013. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12649.
Full textConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
Despite advances in antibiotic therapy, bacterial meningitis (BM) remains with high mortality and morbidity rates in worldwide. One important mechanism associated to sequels during disease is the intense inflammatory response which promotes an oxidative burst and release of reactive oxygen species, consequently leading to cell death. Activation of DNA repair enzymes during oxidative stress has been demonstrated in several neurological disorders. APE1/Ref-1 is a multifunctional protein involved in DNA repair and plays a redox function on transcription factors such as NFkB and AP-1.The aim of this study was assess the role of APE1/Ref-1 on inflammatory response and the possibility of its modulation to reduce the sequels of the disease. Firstly it was performed an assay to measure cytokine in cerebrospinal fluid of patients with BM due to Streptococcus pneumoniae and Neisseriae meningitides. Further, a cellular model of inflammation was used to observe the effect of the inhibition of the endonuclease and redox activity of APE1/Ref-1 on cytokine levels. Additionally, APE1/Ref-1 expression in cortex and hippocampus of rat with MB after vitamin B6 treatment was evaluated. Altogether, results showed a similar profile of cytokines in the cerebrospinal fluid of patients from both pathogens, although IFNy showed higher expression in patients with BM caused by S. pneumoniae. On the other hand, inhibitors of APE1/Ref-1 reduced cytokine levels, mainly TNF-?. Reduction of oxidative stress markers was also observed after introduction of inhibitors in the LPS-stimulated cell. In the animal model, BM increased the expression of the protein APE1/Ref-1, while vitamin B6 promoted reduction. Thereby, this data rise important factors to be considered in pathogenesis of BM, e.g., IFNy can be used as prognostic factor during corticosteroid therapy, APE1/Ref-1 can be an important target to modulate the level of inflammation and VIII oxidative stress, and vitamin B6 seems modulates several proteins related to cell death. So, this study highlights a new understanding on the role of APE1/Ref-1 on the inflammation and the oxidative stress during inflammation condition
A meningite bacteriana (MB) ? uma doen?a infecciosa que permanece com altas taxas de mortalidade e morbidade em todo o mundo, principalmente em pa?ses subdesenvolvidos, apesar dos avan?os na antibioticoterapia. Um dos principais mecanismos associados ?s sequelas durante a MB ? a elevada resposta inflamat?ria, que promove uma exacerbada quantidade de esp?cies reativas de oxig?nio (ERO) levando ?s c?lulas a apoptose ou necrose. A ativa??o de enzimas de reparo de DNA durante o estresse oxidativo tem sido demonstrada nas mais diversas desordens. Uma importante enzima envolvida neste processo ? a endonuclease apur?nica/apirimidinica1/fator redox-1 (APE1/Ref-1). Ela ? uma prote?na multifuncional envolvida no reparo de DNA e na redu??o de fatores envolvidos com a resposta inflamat?ria, tais como o fator nuclear kappa B (NFkB) e prote?na ativadora 1 (AP-1). Este estudo teve como objetivo identificar o envolvimento de APE1/Ref-1 na resposta inflamat?ria visando a possibilidade de sua utiliza??o como alvo terap?utico na redu??o de sequelas durante a MB. Para isto, inicialmente foi realizado uma an?lise no perfil de express?o de citocinas em l?quor de pacientes com meningite causada por Streptococcus pneumoniae e Neisseriae meningitidis visando selecionar moduladores inflamat?rios de interesse para ensaios em cultura de c?lula subsequentes. Em seguida, utilizando um modelo celular de indu??o com LPS foi avaliado o efeito da inibi??o da atividade de reparo e redox de APE1 sobre a express?o de citocinas inflamat?rias. Por fim, foi observada a express?o de APE1 no c?rtex (CX) e hipocampo (HC) de ratos com MB frente a uma terapia adjuvante com vitamina B6. Nossos resultados mostraram um perfil de moduladores inflamat?rios muito semelhante no l?quor dos pacientes com MB causada pelos pat?genos estudados, embora interferon gama (IFNy) tenha sido VI significativamente mais expresso em pacientes com S. pneumoniae do que N. meningitidis. Quanto ao uso dos inibidores das fun??es, redox e de reparo, de APE1/Ref-1 no modelo in vitro, houve redu??o significativa na express?o de algumas citocinas, principalmente o fator de necrose tumoral-alfa (TNF-?). Al?m disso, os inibidores demonstraram uma redu??o nos n?veis de ERO nas c?lulas estimuladas com LPS. No modelo animal, a express?o prot?ica de APE1/Ref-1, no CX e HC dos ratos, foi modulada ap?s introdu??o da vitamina B6. Portanto, esses dados fornecem um novo olhar para a fisiopatologia da MB, em que citocinas como IFNy podem ser usadas em um diagn?stico diferencial entre meningites causadas por S. pneumoniae e N. meningitidis. A prote?na de reparo de DNA, APE1/Ref-1, parece ser um alvo potencial na modula??o da resposta inflamat?ria e do estresse oxidativo, bem como a terapia adjuvante com vitamina B6 mostra ter um papel sobre a express?o de APE1/Ref-1. Consequentemente, o conhecimento obtido neste estudo pode ser importante na melhoria do progn?stico da MB, al?m de contribuir para entender a associa??o entre o reparo de DNA e inflama??o
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Turner, James P. "A case control study of the carpal tunnel syndrome, with special reference to vitamin B6 status." Thesis, University of Surrey, 1989. http://epubs.surrey.ac.uk/848093/.
Full textBhunthurat, Anurak. "The Vitamin B-6 Status of Patients with Chronic Obstructive Pulmonary Disease." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc500541/.
Full textZolfaghari, Sara S. "The relationship between folic acid, vitamin B12, and vitamin B6 intakes and depression in women who use hormonal oral contraceptives." Thesis, California State University, Long Beach, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=1604887.
Full textDepression is a leading cause of disability and mortality worldwide, especially for women. No nutrition recommendations exist for depression. Oral contraceptives (OCs) have become the leading form of pregnancy prevention in the United States. Studies have associated OC use with impaired nutrient status, specifically folate, vitamin B12, and vitamin B6, which also affect brain functions. Dietary folate, vitamin B12, and vitamin B6 self-reported intakes were used to determine the relationship between depression in women who used OCs (n = 34) in a selected cohort ( n = 409) from the National Health and Nutrition Examination Survey, 2003–2008. OC users were more depressed than non-OC users; depression was associated with various quartile levels of vitamin intake (p <.001). No benefit was observed with intakes which exceeded RDAs for non-OC users; OC users were less depressed when intakes exceeded RDAs for folate, vitamin B12, and vitamin B6 by 13%, 75%, and 7%, respectively.
Kruse, Vera [Verfasser], and Rima [Akademischer Betreuer] Obeid. "Auswirkung einer Folat-, Vitamin B12- und Vitamin B6-Supplementation auf den Knochenstoffwechsel zusätzlich zur Basistherapie mit Vitamin D und Kalzium / Vera Kruse. Betreuer: Rima Obeid." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2014. http://d-nb.info/1057869171/34.
Full textSandmark, Jenny. "Enzymatic mechanisms in biotin synthesis: vitamin B₆ catalysis and phosphoryl transfer /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-609-x/.
Full textPotter, Kathleen. "The effects of long-term homocysteine-lowering treatment with folic acid, vitamin B6 and Vitamin B12 on vascular structure and function in stroke." University of Western Australia. School of Medicine and Pharmacology, 2009. http://theses.library.uwa.edu.au/adt-WU2010.0020.
Full textWodarra, Juliane [Verfasser]. "Einfluss von Blutspenden auf Vitamin B6, Vitamin B12, Folsäure, Biotin und Homocystein in Abhängigkeit von Vitamineinnahme, Lebens- und Ernährungsgewohnheiten / Juliane Wodarra." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2009. http://d-nb.info/1023783266/34.
Full textKronenberger, Thales. "Implicações estruturais de mutantes da piridoxal quinase de Plasmodium falciparum." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-12082014-140851/.
Full textVitamin B9 metabolism is a known drug-target for malaria. Vitamin B6 was validated as essential for the parasite. We analysed biochemically and structurally the plasmodial dimeric pyridoxal kinase. PfPdxKs allowed us to determine the localisation of the active site as well the interface between the two monomers and to identify the involved residues. Molecular dynamics shows that the PdxKs dimerization is important for the active sites stability, which was confirmed by the decrease of activity in mutants related to this region. Gel filtration revealed equilibrium of monomer-dimer conformation and therefore the interface mutations decrease in activity might not be directly related to the dimerization processes, but rather to the active site organisation. The active site region shows a serine involved in keeping the pyridoxals conformation during catalyses and the identified lid region covering the ATP binding site is responsible for preventing auto-hydrolysis. Substitution of the respective amino acid to alanine resulted in enzyme inactivation. In silico analysis of the PfPdxK spacer region identified nucleic acid binding sides, however RNA binding experiments failed so far and the possibility of protein-RNA binding remains for elucidation.
Hobbs, Lisa M. "Dietary vitamin B6 supplementation promotes the growth of 7,12-dimethylbenz(a)anthracene-induced mammary carcinoma in Sprague Dawley rats." Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/44023.
Full textMaster of Science
Powell, Lisa. "Effects of various diets on vitamin B-6 and cholesterol levels in ten men aged 21-37." Virtual Press, 1990. http://liblink.bsu.edu/uhtbin/catkey/722433.
Full textDepartment of Home Economics
Cowing, Brandy Ellen. "Vitamin B6 Decreases Proliferation and DNA Synthesis in Human Mammary Carcinoma Cell Lines In Vitro." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/31644.
Full textMaster of Science
Baumgart, Anna [Verfasser]. "Die Bedeutung von Vitamin B6-Defizienzen für ätiologisch ungeklärte Epilepsien des frühen Kindesalters / Anna Baumgart." Kiel : Universitätsbibliothek Kiel, 2017. http://d-nb.info/113823401X/34.
Full textChan-Huot, Monique [Verfasser]. "Modeling the properties and function of the enzymic cofactor vitamin B6 by NMR / Monique Chan-Huot." Berlin : Freie Universität Berlin, 2011. http://d-nb.info/1025509943/34.
Full textRichts, Björn [Verfasser]. "Vitamin B6 metabolism and underground metabolic routes in the Gram-positive bacterium Bacillus subtilis / Björn Richts." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://d-nb.info/1237633621/34.
Full textGrün, Ingolf. "Determination of vitamin B-6, available lysine and pyridoxyllysine in a new instant baby food product." Thesis, Virginia Tech, 1989. http://hdl.handle.net/10919/45974.
Full textThe purpose of this study was to compare the nutrient content of a new instant baby food product to jar baby food of similar product formulation. Instant and jar "Vegetable and Beef" and "Bananas" products processed in 1985 and 1987 were analyzed for available lysine, vitamin B-6 and pyridoxyllysine content.
The available lysine content of 100 grams of baby food was found to be higher in the instant products, but when adjusted for protein content, available lysine was higher in the jar products. This indicates that drum-drying used for the instant products is more detrimental in regard to lysine availability than retorting. The vitamin B-6 content of the instant products was found to be higher than that of the jar products. However, due to the addition of ingredients with little or no vitamin B-6 content to the jar products, no conclusion about processing effects on vitamin B-6 content can be made. Products processed in 1985 tended to be lower in nutrient content than the products processed in 1987. Pyridoxyllysine, a compound thought to affect vitamin B-6 bioavailability, could not be detected in any of the baby foods, either by amino acid or HPLC analysis.
The instant products were found to be at least equal to the jar products with regard to available lysine and vitamin B-6 content. All products also appear to provide sufficient amounts of these nutrients to infants less than one year of age.
Master of Science
Beg, Mohammed Asim. "Host-parasite relationships in vitamin B6 deficient cotton rats (Sigmodon hispidus) infected with Litomosoides carinii (Nematoda, filarioidea)." Thesis, University of Salford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386552.
Full textYamamura, Ei-tora. "Biochemical and molecular biological studies on enzymatic synthesis of vitamin B6 derivatives and optically active carboxylic acids." Kyoto University, 2020. http://hdl.handle.net/2433/245820.
Full text0048
新制・論文博士
博士(農学)
乙第13305号
論農博第2880号
新制||農||1074(附属図書館)
学位論文||R2||N5242(農学部図書室)
富山大学大学院理工学研究科生命環境科学専攻
(主査)教授 小川 順, 教授 阪井 康能, 教授 栗原 達夫
学位規則第4条第2項該当
Rosenberg, Jonathan. "Vitamin B6 Production in Bacillus subtilis." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E32C-B.
Full textRueschhoff, Elizabeth Ellen. "Vitamin B6 metabolism in Arabidopsis thaliana." 2009. http://www.lib.ncsu.edu/theses/available/etd-09032009-121530/unrestricted/etd.pdf.
Full textChen, Wen-shan Chou 1964. "Effect of glycosylated vitamin B-6 intake on the excretion of vitamin B-6 in women." Thesis, 1992. http://hdl.handle.net/1957/27185.
Full textGraduation date: 1993
Ju, Hsiao Li, and 蕭莉茹. "Vitamin B6 intakes and status to evaluatethe vitamin B6 requirement of 13-18 years-aged adolescents in Tainan." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/10768447157720788288.
Full text國立成功大學
生物學系
90
Abstract The characteristic growth rate in adolescence raises requirement for nutrients, thus affecting the nutritional status, especially of vitamin B6 due to its role in the protein metabolism. Studies regarding vitamin B6 status are limited in Taiwan. None was conducted for the adolescents. Therefore, the purpose of this study was to evaluate the vitamin B6 requirement of adolescents aged 13-18 years in Tainan. The subjects participating in the study were 146 boys and 145 girls (a total of 291) who were recruited by stratified random sampling from 27 middle schools and 19 high schools. Daily intakes of vitamin B6 and protein were analyzed according to the 3-day dietary record including 2 weekdays and 1 weekend day. Plasma concentrations of pyridoxal-5-phosphate (PLP) and 24-hrs of urinary 4-pyridoxic acid (4-PA) excretion were analyzed by HPLC. Erythrocyte transaminases activity coefficients (EALT-AC and EAST-AC) were also analyzed. Anthropometric measurements of adolescents (height, body weight, midarm circumference and body fat percentage) were collected by a trained technical people. Recommendations for vitamin B6 intakes were suggested on the basis of the effects of vitamin B6 intake on plasma PLP, EALT-AC, EAST-AC and urinary excretion of adolescents. The Estimated Average Requirements (EAR) for vitamin B6 were determined and the Recommended Dietary Allowance (RDA) for vitamin B6 were calculated according to the methodology of Dietary Reference Intakes (RDIs). Anthropometric measurements of 291 adolescents in this study were similar to or slightly higher than those of NAHSIT (Nutrition and Health Survey in Taiwan) 1993-1996, indicating the normal growth and development. Among these, 230 (110 boys and 120 girls) completed the dietary and biochemical study. All subjects had plasma PLP > 20 n mol/L. Urinary 4-PA ≦3 mmol/ day were observed in 5.00 ﹪boys and 12.00 ﹪girls. EALT-AC≧1.25 were observed in 20.00 ﹪boys and 28.00 ﹪girls. EAST-AC≧1.80 were observed in 24.00 ﹪boys and 40.00 ﹪girls. These values are considered indicatives of vitamin B6 inadequacy. Mean daily intakes of energy, protein and vitamin B6 were significantly higher in boys than girls. Plasma PLP concentration and urinary 4-PA excretion of the boys were significantly higher than the girls. The percentages of inadequacy for EALT-AC and EAST-AC were significantly higher in girls than in boys. Mean daily intakes of energy and protein did not differ significantly between 13-15 and 16-18 years aged groups. Mean daily intake of vitaminB6 for 16-18 years was significantly higher than 13-15 years-aged. Plasma PLP concentration did not differ significantly between 13-15 and 16-18 years aged groups. Urinary 4-PA excretion of the adolescents aged 16-18 years was significantly higher than that of 13-15 years-aged. The percentages of inadequacy for EALT-AC and EAST-AC of 13-15 years were significantly higher than those of 16-18 years-aged. In conclusion, vitamin B6 status of adolescents aged 13-18 years showed the inadequacy in the short-term intakes of vitamin B6 reflected by the inadequate urinary 4-PA excretion observed in 5-12 % of adolescents in this study. All subjects had plasma PLP >20 n mol/L indicating that vitamin B6 intakes were adequate to meet physiological metabolism within 7-10 days. However, vitamin B6 status was suggested to be inadequate for long-term (6-8 wks) due to the high percentages (20-40%) of inadequacy for EALT-AC and EAST-AC. The Estimated Average Requirements for vitamin B6 which of satisfy biochemical indicators in plasma, erythrocyte and urine were determined and used to calculate Recommended Dietary Allowances (RDAs) by multiplying 120%. This study suggested that RDAs for vitamin B6 were 1.20 mg and 1.28 mg for boys aged 13-15 and 16-18 years, respectively. The RDAs for vitamin B6 were 1.04 mg and 1.16 mg for girls aged 13-15 and 16-18 years, respectively.
Hou, Hui-Ching, and 侯慧卿. "Inflammation alters vitamin B6 metabolism in vivo." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/p9z4pf.
Full text國立中興大學
食品暨應用生物科技學系所
102
Abstract Rheumatoid arthritis (RA) is a systemic autoimmune disease with unclear etiology and pathogenesis. Pyridoxal 5’-phosphate (PLP) is the major B6 vitamins form, and a variety of disease conditions have repeatedly been found to be associated with low levels of plasma PLP. Inverse associations have been shown between plasma PLP and biomarkers of human inflammation. Collagen-induced arthritis (CIA) and adjuvant-induced arthritis (AA) rats are commonly used as models for studying human with rheumatoid arthritis (RA). The objective of the present study is to investigate vitamin B6 metabolism during inflammation in vivo. Twenty-two 6-week-old female Lewis rats were housed in individual IVC cages under pathogen-free condition on a 12-hour day/night cycle and fed with the AIN93M diet during the experimental period. Arthritis was induced at the age of 10-week and were sacrificed at the age of 15-weeks during chronic inflammation. The volume of paw significantly increased in both AA and CIA groups during inflammation while body weight significantly decreased. Arthritis induction did not alter 24-hour urinary excretion of vitamin B6 in both models. However, inflammation resulted in drastic alterations in vitamin B6 in both liver and certain extra-hepatic tissues except for the spleen. The lower circulating plasma PLP levels observed in the CIA group reflected a decrease in hepatic PLP contents, as a positive correlation was observed between the two (r=0.731 p=0.039, n=8). The mechanism by which arthritis causes vitamin B6 mobilization during inflammation is under investigation.
Chang, Hsin-Yueh, and 張馨月. "Vitamin B6 status and metabolism in rheumatoid arthritis." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/38806691068629835441.
Full text國立中興大學
生物科技學研究所
99
Rheumatoid arthritis (RA) has been related with low vitamin B6 status and impaired vitamin B6 function. Three enzymes are involved in the metabolism of vitamin B6 in different tissues. These enzymes are pyridoxal (pyridoxamine, pyridoxine) phosphate phosphatase (PDXP), pyridoxal (pyridoxamine, pyridoxine) kinase (PDXK) and pyridoxine (pyridoxamine) phosphate oxidase (PNPO). There is the possibility that inadequate vitamin B6 levels in RA patients might be result from abnormal vitamin B6 metabolism. The purpose of this study is to examine these enzymes expression in vitamin B6 metabolism and disclose the relationships between these enzymes and vitamin B6 levels in circulating and tissues. In human study, we investigated the plasma and erythrocyte B6 levels in RA patients and controls. Hepatic PDXP gene expressions and hepatic PDXK enzyme activity in animals were affected by prednisolone treatment. In addition, plasma B6 levels were affected in mice by long-term prednisolone treatment. Furthermore, prolonged nonsteroidal antiinflammatory drugs (NSAIDs) treatment attenuated hepatic, kidney and erythrocyte B6 levels in animals. Moreover, it should be concerned about long-term NSAIDs use because their adverse effect on attenuating vitamin B6 status.
D'ALESSIO, FEDERICO. "Regulation of vitamin B6 metabolism in Escherichia coli." Doctoral thesis, 2020. http://hdl.handle.net/11573/1470147.
Full textMASCOLO, Elisa. "Relationship between Vitamin B6, DNA damage and diabetes." Doctoral thesis, 2021. http://hdl.handle.net/11573/1531464.
Full textSindihebura-Ruhumba, Pascaline. "Effect of controlled vitamin B-6 intake and pyridoxine supplementation on B-6 status of smokers." Thesis, 1999. http://hdl.handle.net/1957/27213.
Full textGraduation date: 1999
Witzinger, Linda. "Rolle der Pyridoxal 5´-Phosphat Phosphatase PDXP im Vitamin B6-Metabolismus muriner Erythrozyten und Hippocampi." Doctoral thesis, 2020. https://doi.org/10.25972/OPUS-21654.
Full textThe phosphatase PDXP, also called Chronophin, is a member of the ubiquitously expressed HAD-phosphatases, which have some important physiological functions in the organism. Its substrate pyridoxal 5´-phosphate (PLP) is the active form of vita-min B6, an important cofactor of several reactions. PDXP-deficient mice (KO-mice) have PLP-concentrations in erythrocytes and in the whole brain twice as high as wildtype mice. It is assumed that PDXP therefore has an important function in erythrocytes and in the brain. The aim of the study was to gain initial insights into these functions of PDXP. For this purpose, HPLC-based analyses of the PLP-concentrations in erythrocytes from WT- and KO-mice were carried out. The doubled PLP-levels in the RBCs of KO-mice could be confirmed. In addition, a method for measuring the endogenous phosphatase activity of PDXP in red cell lysates was established. The activity of PDXP could be measured by the reduction of its substrate PLP over time. This required the incubation with pyridoxine and the inhibition of PDXK by ginkgotoxine. An assumed function of PDXP in mobilization of PL(P) from the erythrocytes in fasting conditions could be ruled out. Therefore, a comparison between the PLP-concentrations in RBCs of fasted mice with normal fed ones was done. Surprisingly the fasted KO-mice showed the same percentaged decrease of cellular PLP-level as the fasted WT-mice. During vitamin B6 intake however, a function of PDXP as being a “converter” of pyridoxine to pyridoxal was found. Starting with PN, a PDXP-mediated dephosphorylation from PLP to PL could take place in the wildtype mice (via the intermediate steps PNP and PLP). Consequently, the WT´s production of PL quadrupled compared to the KO´s. PDXP turned out to be essential for the conversion of pyridoxine to pyridoxal in erythrocytes. This conversion confers some metabolic flexibility to the organism and to a certain extent makes it independent of the choice of food. Moreover, cells and organs, that due to the absence of PNPO cannot produce PL(P) themselves, can be provided via erythrocytes. The high reactivity of PLP with surrounding nucleophiles poses a certain problem for the cell in dealing with free PLP. The majority of the PLP in RBCs is bound to proteins (primarily hemoglobin). It was distinguished between the here termed “free” PLP and the bound PLP by using filter devices with a MWCO at 3 kDa. First insights could be gained about PDXP as a determinant of free PLP-levels in erythrocytes and hippocampus. The amount of free PLP in the hippocampus was significantly higher than in the RBCs. Additionally, the hippocampus showed some differences in the con¬centration of free PLP between WT- and KO-mice. The level of free PLP in PDXP deficient mice was one third higher than in wildtype mice. Finally, some correlation between the age of the mice and their PLP-metabolism was found. The results revealed changes of the PLP-concentrations with age in the RBCs and the hippocampus. Moreover, western blot analyses showed some age-related differences in the expression of vitamin B6 enzymes. In the hippocampus older wildtype mice showed a quintupled expression of PDXP compared to younger ones. However, western blot analyses of red blood cell lysates from older animals revealed a lower expression of PNPO by a factor of four. For the organism this physiological reduction of its PNPO expression with age would mean a loss of metabolic flexibility, that is accompanied by the conversion from PN to PL
Lin, Sang-Chu, and 林桑竹. "Effects of Glutamine on Vitamin B6 Biosynthesis in Arabidopsis thaliana." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/92987756308143317370.
Full text國立臺灣大學
植物科學研究所
104
Glutamine is one of the most abundant free amino acids in plants. As the first organic nitrogen from primary nitrogen assimilation, glutamine is a major amino donor for the synthesis of amino acids, nucleotides, and other nitrogen-containing compounds. We showed that glutamine could efficiently support Arabidopsis growth when used as a sole nitrogen source in the growth medium, but the addition of excess glutamine significantly inhibited Arabidopsis growth. We thus used appropriate glutamine concentration as sole nitrogen source to screen for “glutamine hypersensitive” mutants. One of the mutants, line 23844/pdx3-3, had dramatically reduced root growth, irregular cell arrangement, less cell proliferation and abnormal cell death in glutamine-containing medium. Line 23844 is defective in At5g49970 that encodes pyridoxine/ pyridoxamine phosphate oxidase (PDX3) of the vitamin B6 salvage pathway. The mutant has a point mutation that changes Gln226 to a stop codon. In addition to the mutant, we have obtained the genetic complementation line and PDX3 gene silencing lines that show similar phenotypes. These results suggested that the growth defects in 23844 are caused by loss-of-function in the PDX3 gene. sos4 (salt overly sensitive 4), another mutant involved in vitamin B6 salvage pathway, had similar phenotypes with pdx3-3, and pdx3-3/sos4 double mutant had more severe phenotypes. Furthermore, by analysis vitamin B6 contents in pdx3-3 and sos4, we found abnormal vitamin B6 levels in mutants. These data indicated that defects in vitamin B6 salvage pathway might result in glutamine hypersensitive phenotypes. Nevertheless, the functions of PDX3 and the vitamin B6 salvage pathway are not well understood in plants. We will further study the relationship between glutamine and vitamin B6 salvage pathway, and the regulation of vitamin B6 homeostasis.
Bills, Nathan D. "In vivo and in vitro determination of the bioavailability of vitamin B-6 from plant foods containing pyridoxine glucoside." Thesis, 1990. http://hdl.handle.net/1957/25980.
Full textKwak, Ho-Kyung. "Effect of varying levels of vitamin B-6 intake on lymphocyte mitogenic response and vitamin B-6 concentration in human peripheral blood mononuclear cells." Thesis, 2001. http://hdl.handle.net/1957/26568.
Full textGraduation date: 2002
Hansen, Christine M. 1953. "Effect of vitamin B-6 intake, protein intake and bioavailability on vitamin B-6 status for women." Thesis, 1995. http://hdl.handle.net/1957/27152.
Full textGraduation date: 1996