Dissertations / Theses on the topic 'Viral movements'
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Lin, Jun. "Structures of Poliovirus and Antibody Complexes Reveal Movements of the Capsid Protein VP1 During Cell Entry." BYU ScholarsArchive, 2011. https://scholarsarchive.byu.edu/etd/3047.
Full textHerent, Coralie. "Respiratory Adaption to Running Exercise : A Behavioral and Neuronal Circuits Study in Mice Absent Phasing of Respiratory and Locomotor Rhythms in Running Mice Control of Orienting Movements and Locomotion by Projection-Defined Subsets of Brainstem V2a Neurons Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection." Thesis, université Paris-Saclay, 2021. http://www.theses.fr/2021UPASL001.
Full textDuring running, ventilation increases to match the augmented energetic demand. Yet the presumed neuronal substrates for this running hyperpnea have remained elusive. To fill this gap, we have, in mice, examined the interactions between i) limb movements and respiratory cycles, and ii) locomotor and respiratory neural networks. First, by combining electromyographic recordings (EMG) of the diaphragm with limb video-tracking in running mice, we show that, for a wide range of trotting speeds on a treadmill, breathing rate increases to a fixed value, irrespective of running speeds. Importantly, breaths are never temporally synchronized to strides, highlighting that exercise hyperpnea can operate without phasic signals from limb sensory feedbacks. We next sought to identify candidate trigger neurons in the locomotor central network, and their partners in respiratory centers. Combining EMG recordings, viral tracing, and activity interference tools, we first show that the prime supraspinal center for locomotor initiation (the mesencephalic locomotor region, MLR) can upregulate breathing during, and even before, running. Indeed, the MLR contacts directly and modulates the main inspiratory generator, the preBötzinger complex. We show that the lumbar locomotor circuits also have an excitatory action onto respiratory activity, but that this ascending drive targets another essential respiratory group, the retrotrapezoid nucleus. This work highlights the multifunctional nature of locomotor command and executive centers, and points to multiple neuronal pathways capable of upregulating breathing during, or possibly even prior to, running
Seaberg, Bonnie Lee. "Host factors involved in viral movement through plants." Thesis, [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-3282.
Full textEdge, David. "Identification of host factors controlling plant viral movement." Thesis, University of East Anglia, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.398793.
Full textPivo, Trevor Raye. "Movement of a Viral Surrogate from Restrooms to Public Areas in a Hospital." Thesis, The University of Arizona, 2016. http://hdl.handle.net/10150/613369.
Full textGermundsson, Anna. "Transgenic resistance to PMTV and PVA provides novel insights to viral long-distance movement /." Uppsala : Dept. of Plant Biology And Forest Genetics, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/2005105.pdf.
Full textPeiró, Morell Ana. "Proteínas de movimiento de la familia 30K:interacción con membranas biológicas y factores proteicos y su implicación en el transporte viral." Doctoral thesis, Universitat Politècnica de València, 2015. http://hdl.handle.net/10251/48471.
Full textPeiró Morell, A. (2014). Proteínas de movimiento de la familia 30K:interacción con membranas biológicas y factores proteicos y su implicación en el transporte viral [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/48471
TESIS
Dall'Ara, Mattia. "RNA/RNA interactions involved in the regulation of Benyviridae viral cicle." Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAJ019/document.
Full textMultipartite RNA virus condition to provide a complete set of genomic segments in each infected cell implies a high level of MOI that results in an unsustainable biological cost in terms of viral replication. In the proposed model, to minimize the cost of the genome integrity preservation, a network of RNA/RNA interactions determines the recognition and the mobilization of at least one of each genomic RNAs in a modular RNP complex. Such complex must be considered as the mobile infectious unit of the segmented genome during viral spread in the plant. The Aim of this thesis was to experimentally determine the existence of RNA/RNA interactions between BNYVV RNAs and their implication in the viral cycle. BNYVV genomic segments have been co-detected within isolated single cells from systemic tissues where they accumulate to reach set point genome formulas. In the model where vRNAs interact each other to form the minimal mobile infective unit, RNA1 and RNA2 interaction domain has been identified in silico and in vitro. The rationale of such an interaction has been provided in vivo using BNYVV and Beet soil-borne mosaic virus chimeras
Fernandes, Nelson Horta. "The effect of habitat encroochment by roads on space use and movement patterns of an endangered vole." Master's thesis, Universidade de Évora, 2019. http://hdl.handle.net/10174/25598.
Full textHipper, Clémence. "Nature du complexe viral impliqué dans le mouvement à longue distance du virus de la jaunisse du navet." Thesis, Strasbourg, 2013. http://www.theses.fr/2013STRAJ063/document.
Full textIn the project, Turnip yellows virus (TuYV) transport in the phloem was analysed. The first objective was to identify the nature of the viral complex involved in vascular movement: virions and/or ribonucleoprotein complexes. Mutant viruses were modified in the capsid protein gene to inhibit formation of virions. By analyzing their movement in different host plants, in the absence or in the presence of the wild-type capsid proteins brought in trans, we demonstrated a strong relation between virion formation and virus long-distance movement. The second objective was to identify cellular partners of the TuYV-P4 protein, a putative movement protein which is host-specific. Two proteins were identified by screening a cDNA library of A. thaliana using the yeast two hybrid technique, and their function in the virus cycle was assessed by performing sub-cellular localizations and infection of A. thaliana KO mutants
Li, Changzhi. "Doppler phase modulation effect for non-contact accurate measruement [sic] of vital signs and other periodic movements from theory to CMOS system on chip integrations /." [Gainesville, Fla.] : University of Florida, 2009. http://purl.fcla.edu/fcla/etd/UFE0024294.
Full textFlobinus, Alyssa. "Rôle de la protéine p14 du BNYVV et de l'ARN-3 viral dans la suppression de l'interférence par l'ARN et le mouvement à longue distance." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAJ045/document.
Full textThe beet necrotic yellow vein virus (BNYVV) is a multipartite positive-stranded RNA phytovirus. The RNA3 contains a « core » sequence in which resides the « coremin » motif of 20 nucleotides absolutely required for the viral systemic movement in Beta macrocarpa. The RNA3 undergoes a process that produces a noncoding RNA3 (ncRNA3), stabilized by « coremin » at its 5’ end. Using a yeast genetic approach, the exoribonuclease Xrn1 and plant XRN4 have been identified as being responsible for the ncRNA3 accumulation from RNA3 processing. In vitro, we showed that the ncRNA3 accumulation is due to the stalling of Xrn1 by “coremin”. The viral p14 protein, an RNA silencing suppressor encoded by the RNA2, is also required for the systemic movement and interacts with the “coremin” sequence. Our studies demonstrated the ability of RNA3 to partially complement an allelic p14 mutant in local and systemic infections. Our data highlighted an effect of the p14 protein on the RNA silencing movement and on the potential cellular target RDR6
Boissinot, Sylvaine. "Partenaires et rôle dans le cycle viral des différentes formes de la protéine RT du Cucurbit aphid-borne yellows virus." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-00998392.
Full textLinhares, José Carlos Tarrio. "Clínica e cirurgia em bovinos de aptidão leiteira." Master's thesis, Universidade de Évora, 2015. http://hdl.handle.net/10174/17548.
Full textPoignavent, Vianney. "Relations structure-fonctions chez la protéine multi-fonctionnelle P1 du virus de la panachure jaune du riz." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS024.
Full textThe virus of rice yellow mottle virus (RYMV for Rice Yellow Mottle Virus) mainly infects the genus Oryza and causes significant damage to rice crops in Africa. Although its genome is rudimentary, this virus code essential proteins for its maintenance in the host despite the defense mechanisms of the plant. Recent work by the team has identified the P1 protein encoded by the virus as a protein that could, through its ownership of RNA silencing suppressor, allow the virus to bypass an essential defense mechanism of the host and allow the virus to perpetuate its viral cycle. Little data on the mechanisms of action of the P1 protein is available to date. The work undertaken during my thesis was therefore to supplement the knowledge of the biochemistry of this protein, to define its three-dimensional structure and update its sub cellular localization to reveal properties that could enable us not only to understand how this protein works its functions but also to define methods of adequate response against the virus. Thus, I show that the P1 protein is a new zinc finger protein family having a unique 3D structure consisting of a first domain involved in the dimerization of the protein and in interactions with ligands some of which may originate from the plant host. My work also identifies a second sensor field in the redox state of the protein that probably allows him to probe the state of the plant during viral infection and adapt its conformation to conduct their duties. Finally, a mutagenesis approach to P1 assisted by the new 3D protein structure shows that it is now possible to identify critical residues in the protein for its participation in the viral infection. This work thus opens up many possibilities for future mechanistic studies on these key areas of the protein, as well as for studies of genetic diversity within many RYMV isolates of virus in Africa
Soares, Elizângela Teixeira. "Quando a leste vira centro: o desenvolvimento econômico da Zona Leste na perspectiva dos atores do Fórum para o Desenvolvimento da Zona Leste." Pontifícia Universidade Católica de São Paulo, 2016. https://tede2.pucsp.br/handle/handle/19012.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The East Zone of the city of São Paulo has been put in evidence on national and international news, with an increasing in 2010, when the district of Itaquera was chosen to host the opening of the World Cup soccer games 2014. The history of this region shows that it has been considered a commuter region, as it is opposed to the West Zone, the most developed zone of São Paulo and where the private and public investments are destined. Hosting the World Cup openings not only boosted roads, urban interventions, and the implementation of social facilities, but has also put in evidence again the struggle of the development of the East Zone. The present study was created in order to identify how the social characters of the region have organized on the face of such development process and how they have mobilized to take ownership of it. It was decided to start the study by the Forum for the East Zone Development (FDZL) and, from the network analysis methodology, questioning what kind of development are suitable for the region. The initial hypothesis stated that the Forum was articulating as a cohesive group, helping the development of the East Zone. However, the study showed that the Forum is a heterogeneous network, which on the moment of the competition for development was disjointed. The reorganization of the network intended to find the characters and identify exactly if it can demonstrate what I pointed out in relation to networks, that they are different players disputing for the same social space. The network shows that they do. Thus, the study revealed that the present conflict over the East Zone development is a dispute between organizations and social movements, such as the Forum organization, and what is on dispute is whether or not the East Zone will be constituted as a centrality
A Zona Leste de São Paulo ganhou evidência nos noticiários nacionais e internacionais, principalmente a partir de 2010, quando Itaquera foi o distrito escolhido para sediar o estádio de abertura da Copa do Mundo de 2014. A história da região demonstra que ela tem sido relegada à condição de cidade dormitório por oposição à Zona Oeste, a mais desenvolvida da cidade de São Paulo e para onde têm sido destinados os investimentos públicos e privados. Sediar o estádio de abertura da Copa não impulsionou apenas as intervenções viárias e urbanas e a implantação de equipamentos sociais, mas também recolocou em evidência a disputa pelo desenvolvimento da Zona Leste. Assim, o presente estudo foi elaborado com o objetivo de identificar como os atores sociais dessa região se organizaram diante desse processo de desenvolvimento e como se mobilizaram para dele se apropriarem. Optou-se por iniciar o estudo por meio do Fórum para o Desenvolvimento da Zona Leste (FDZL) e, a partir da metodologia de análise de redes, questionar que tipo de desenvolvimento desejam para a região. A hipótese inicial indicava que o Fórum era grupo articulador coeso, articulado em função do desenvolvimento da ZL. No entanto, o estudo demonstrou que o Fórum é uma rede heterogênea que, no momento em que a disputa pelo desenvolvimento foi recolada em evidência, estava desarticulada. A reorganização da rede pretendeu, portanto, localizar os atores e identificar, exatamente, se ela consegue demonstrar aquilo que evidenciei em relação às redes, ou seja, que são atores distintos em disputa pelo mesmo espaço social. A rede mostra que sim. Desse modo, o estudo revelou que o conflito presente em torno do desenvolvimento da Zona Leste é uma disputa entre organizações como os movimentos sociais e organizações como o Fórum, e o que está em disputa é constituir ou não a Zona Leste uma centralidade
Mealor, Michael A. "Spatial heterogeneity in ecology." Thesis, University of Stirling, 2005. http://hdl.handle.net/1893/53.
Full textValls, Jimena Paola Hochmann. "Análise do impacto das proteínas E6/E7 de diferentes variantes moleculares de HPV-16 sobre as vias de transdução de sinal mediadas por MAPK." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5155/tde-21092016-084921/.
Full textPersistent infection with HPV-16 is strongly associated with risk of developing neoplasia in the uterine cervix, vagina, vulva, penis, anal canal and oropharynx. The detailed study of HPV-16 intra-typical nucleotide variability resulted in important findings regarding phylogeny and viral evolution, and the natural history of infections. Asian-American (AA) and E-350G variants of HPV-16 were associated with increased risk of persistent viral infection and development of cervical cancer compared to the European prototype (E-P or E-350T), although this variant still presents higher risk when compared to other viral types. More recently, functional differences between the E6/E7 proteins of distinct molecular variants of HPV-16 are being described, in order to explain the differences in the epidemiological associations observed. Data from our group pointed to increased transcription of the MEK2 gene specifically in primary human keratinocytes (PHKs) transducing E6/E7 of the E-350G variant. Consequently, the aims of this study were: 1) To examine the activation levels of effector proteins of the signal transduction pathways mediated by MAPK and PI3K/AKT in PHKs immortalized by E6/E7 of three different molecular variants of HPV-16 (AA, E-P, E-350G); (2) To analyze the effects of E6/E7 of different molecular variants of HPV-16 upon MAPK pathways concerning the induction of transcription factors; (3) To analyze the transforming potential of PHKs immortalized by different molecular variants of HPV-16, and in cooperation with the cellular protein c- MYC; (4) To analyze the potential of migration and invasion in PHKs immortalized by different molecular variants of HPV-16, and in cooperation with the cellular protein c- MYC. In this study we observed that the AA variant of HPV-16 induced higher activation of both signaling pathways studied (MAPK, and PI3K/AKT). Furthermore, this variant presented increased migration capacity, higher invasion through a collagen matrix, and greater transforming potential. Moreover, cells immortalized by the AA variant showed higher expression of the mesenchymal protein vimentin and a decrease of the epithelial protein E-cadherin, suggesting partial activation of Epithelial Mesenchymal Transition (EMT). In addition, when the c-MYC oncogene was co-transduced in the different cells lines infected with HPV-16 E6/E7, we observed that in PHKs immortalized by the AA variant there was also an enhanced activation of the MAPK-ERK pathway, a higher ability to migrate, and similar transformation potential in comparison with cells co-transduced with the E-350G variant and c-MYC. Taken together, this data suggest that the AA molecular variant of the HPV-16 has a selective advantage over the other variants to promote cell transformation, migration and invasion, and this could partly explain the higher prevalence of this variant in cervical cancer. The results generated in this study are very important to assess the impact of intra-typical variability of HPV-16 on the oncogenic potential observed in epidemiological studies
Agustina, VIra [Verfasser], Kerstin [Akademischer Betreuer] [Gutachter] Martens, and Michael [Gutachter] Dobbins. "PISA, Public Participation and Policy Making in Education : the Impact of Online Social Movements and the Shift in Public Involvement in the Basic and Secondary Education Decision Making Processes in Indonesia / VIra Agustina ; Gutachter: Kerstin Martens, Michael Dobbins ; Betreuer: Kerstin Martens." Bremen : Staats- und Universitätsbibliothek Bremen, 2017. http://d-nb.info/1143765699/34.
Full textRiotte-Lambert, Louise. "Se souvenir et revenir : approche théorique et méthodologique des stratégies de déplacement récursif et de leurs conséquences populationnelles." Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT169.
Full textRecursive movement patterns, by which an individual returns to already visited sites, are very common. Memory use, hypothesized to be advantageous when the environment is predictable, could underlie the emergence of these patterns. However, our understanding of the memory-movement interface has been limited by two knowledge gaps. We still lack appropriate methodologies and theoretical knowledge of the advantages of memory use and of the patterns that emerge from it. During this PhD project, I aimed at filling in some of these gaps. I present here three new frameworks for the analysis of recursive movement patterns. The first one delimits the areas most frequently revisited by an individual, the second one detects periodic revisit patterns, and the third one formally defines and quantifies routine movement behaviour in terms of movement sequence repetitiveness, and presents an algorithm that detects the sub-sequences that are repeated. Using an individual-based model, we show that memory use, when the environment is predictable, is very energetically advantageous compared to foraging strategies that do not use memory, including in a situation of competition, and that it leads to the emergence of stable Home Ranges and spatial segregation between individuals. Memory use invalidates several hypotheses very commonly made in population studies, by leading to a stronger environmental depletion, to a higher equilibrium population size, and to a nonlinear relationship between the total population size and the individually-experienced intensity of competition. Therefore, my PhD thesis contributes to a better understanding of the consequences of memory use for the fitness of individuals, for movement patterns, and for population dynamics. It offers innovative methodologies that quantify and characterize recursive movement patterns that can emerge from its use. These methods should open new opportunities for the comparison of the movements of individuals from different populations and species, and thus the testing of hypotheses about the pressures that select for memory use
Arduini, Guilherme Ramalho 1983. "Em busca da Idade Nova : Alceu Amoroso Lima e os projetos catolicos de organização social (1928-1945)." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/282068.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas
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Resumo: Este estudo trata de um intelectual brasileiro chamado Alceu Amoroso Lima, também conhecido como Tristão do Ataíde. Em 1928, ele assumiu publicamente a fé católica, tornando-se uma das vozes mais importantes da Igreja no Brasil durante as décadas seguintes. Em outras palavras, ele envolveu-se indiretamente em política, tornando-se o representante oficioso da Igreja para assuntos ligados à política e escreveu muitos livros e artigos. Entre as décadas de 1920 e 1940 ele demonstrava uma mentalidade reacionária, mas gradativamente tornou-se um defensor ardoroso da liberdade de imprensa e outros direitos civis, especialmente após a II Guerra Mundial. Nosso objetivo é compreender as causas desta mudança, assim como as conseqüências que isto trouxe para suas ações e escritos sobre o problema da organização das classes trabalhadoras de acordo a Doutrina Social da Igreja.
Abstract: This study is about a Brazilian intellectual called Alceu Amoroso Lima, also known as "Tristão do Ataíde". In 1928, he publicly adopted Catholicism, becoming one of the most important voices of the Church in Brazil throughout the following decades. In other words, he got indirectly involved in politics by becoming the semi-official delegate for political affairs and wrote several articles and books. In the late 1920s and 1930s he expressed a very conservative mindset; nevertheless especially after the II World War he gradually became a champion of freedom of speech and other civil rights. Our aim is to understand the causes of this change as well as the consequences it brought to his actions and writings on the problem of organizing the working classes according to the Catholic Social Doctrine.
Mestrado
Historia
Mestre em História
Saeed, Muhammad. "The role of a geminiviral DNA β satellite in viral pathogenicity and movement." 2006. http://hdl.handle.net/2440/37886.
Full textThesis (Ph.D.)--School of Agriculture, Food and Wine, 2006.
Saeed, Muhammad. "The role of a geminiviral DNA β satellite in viral pathogenicity and movement." Thesis, 2006. http://hdl.handle.net/2440/37886.
Full textThesis (Ph.D.)--School of Agriculture, Food and Wine, 2006.
Chen, Jao-Shien, and 陳昭賢. "A microtubule-associated protein, NbMAP90, is involved in viral movement in Nicotiana benthamiana plants." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/46002523882021435449.
Full text國立中興大學
生物科技學研究所
98
During reproduction, plant viruses need many host factors to assist their transcription, translation, replication, encapsidation and movement etc. In order to investigate the interaction between hosts and viruses, we used Bamboo mosaic virus (BaMV) as the material to screen the genes of Nicotiana benthamiana meaningful for viruses. BaMV belongs to the genus Potexvirus and is a single-stranded, positive-sense RNA virus. We isolated the differentially expressed genes of N. benthamiana after virus infection by cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. Among the up-regulated candidates a gene fragment and its full length cDNA were cloned. The sequence was predicted to encode a 90-kDa microtubule-associated protein product and designated NbMAP90. Immunolocalization assay by confocal microscopy also showed colocalization of NbMAP90 and microtubules. On the other hand, NbMAP90 was predicted to contain a TBC (Tre-2, Bub2p, Cdc16) functional domain, suggesting its possible role in the regulation of intracellular membrane trafficking. For further understanding the roles of NbMAP90 in BaMV replication, we reduced the NbMAP90 mRNA by gene silencing strategy, infected the NbMAP90-knockdown protoplasts and leaves with virus, and assessed viral accumulation by measuring the amount of viral coat protein. There was no significant difference in BaMV coat protein accumulation between the NbMAP90-knockdown protoplasts and the control cells, indicating the viral replication was not interfered by reduction of NbMAP90. However, at 7 days post-inoculation, BaMV coat protein accumulation in the NbMAP90-knockdown leaves was 50% of that in control leaves; however, coat protein in knockdown leaves was 2% of that in control leaves in systemic leaves. Additionally, once the NbMAP90 was knocked down, the viral spreading efficiency was reduced than in the control leaves. We also infected the NbMAP90-knockdown leaves with Potato virus X (PVX) and Cucumber mosaic virus (CMV) and the results also showed coat protein reduction, indicating that effects of this gene on the population accumulation of plant viruses exhibits no species-specificity. By means of these results we suggest that NbMAP90 could be involved in BaMV cell-to-cell movement. The possible mechanism of this protein involved in BaMV life cycle in plants is discussed.
Wang, Chao-Wen, and 王昭雯. "Viral RNA Distribution and Movement of Tomato Bushy Stunt Virus and Cucumber Necrosis Virus in Spinach." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/95629431729218508166.
Full text國立臺灣大學
植物病蟲害學系
85
Systemic infection of plants by viruses requires compatible interactions between viral and cellular factors. Virus must first replicate in the initially inoculated cell and then move to adjacent cells through plasmodesmata. Cell-to-cell movement to phloem, followed by entry into sieve elements, is required for long distance transport to other organs and tissues. Except of virus replication and movement, plant active defense response also determine the viral host range. Two viruses, tomato bushy stunt virus (TBSV) and cucumber necrosis virus (CNV), both belong to tombusvirus group and have highly sequence similarity. The aim of the thesis is to analyze the possible reasons why TBSV systemic infected and CNV localized in spinach plants. Firstly, we prepared spinach protoplast and detect time-course viral RNA accumulation in it. We found that TBSV replication faster than CNV about 2hr. Secondly, in the plant tissue level, we detect viral RNA accumulation by northern hybridization. The data showed that botus (TBSV) and cucumber necrosis virus (CNV), both belong to tombusvirus group and have highly sequence similarity. The aim of the thesis is to analyze the possible reasons why TBSV systemic infected and CNV localized in spinach plants. Firstly, we prepared spinach protoplast and detect time-course viral RNA accumulation in it. We found that TBSV replication faster than CNV about 2hr. Secondly, in the plant tissue level, we detect viral RNA accumulation by northern hybridization. The data showed that both TBSV and CNV RNA can be detected on the day before symptom showing. Thirdly, we use dot blot and tissue print hybridization to show the time-course RNA distribution with tip-inoculation or lateral-inoculation methods. The data showed that TBSV move into vascular bundle and region of the plants. Although CNV is localized in inoculated leaf, and necrotic local lesion formation. However, CNV RNA signals can always be detected outside the necrotic regions. To illustrate the potential application of green fluorescent protein (GFP) reporter gene in viral movement studies, TBSV-GFP and CNV*-GFP were constructed in which near 3'' region of the coat protein gene of wild type viruses were inserted by GFP. Blue light fluorescent microscopy of the inoculated spinach, Nicotiana benthamiana and Chenopodium quinoa plant showed that TBSV-GFP has better fluorescent expression than CNV*-GFP has. In C. quinoa plant. TBSV-GFP showed especially evident green fluorescence, we concluded that in this plant, GFP has most potential to viral movement studies. We tried paraffin sections, and used RNA in situ hybridization method to analyze the two virus distribution in spinach inoculated leaves. The data showed that TBSV signals had ever been seen in every kinds of cells. CNV signals can be detected outside the necrotic lesions and unable to move into the vascular region. According to these dataes, we concluded that why CNV is localized in spinach is because the virus can''t move into the vascular bundle for systemic infections.
Wun, Da-Cian, and 温達乾. "Ipsilateral Dual SIL-Radar System for Vital Sign Detection with Random Body Movement Cancellation." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/44533297795176692810.
Full text國立中山大學
電機工程學系研究所
104
This thesis studies a vital-sign detection system based on a dual self-injection-locked (SIL) radar to resolve the interference due to random body motion, which is one of the challenging issues in this field. The system creates the 180 degrees phase difference between the two different paths in the dual SIL radar to achieve the random body motion cancellation condition in detecting vital signs such as respiration and heart rates. The system architecture and experimental setup are constructed through a step-by-step procedure. Firstly, a random object motion is generated by shaking a metal plate. Secondly, the phase shifter is tuned to cancel the Doppler shift caused by the motion of the object under a well-balanced system architecture. Finally, the dual SIL radar is used to detect vital signs under random body motion with comparison to the signal SIL radar. Only the dual SIL radar can successfully detect the normal and high respiration and heart rates of a human before and after exercise, respectively, with random body motion.
Hung, Wei-Ping, and 洪偉玶. "Real-Time and Noncontact Impulse Radio Radar System for um Movement Accuracy and Vital-Sign Monitoring Applications." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/rw9bsy.
Full text國立交通大學
電信工程研究所
106
In this dissertation, a real-time, contactless pulse-based ultra-wideband (UWB) radar sensor was used to precisely detect the movement of the robotic arm, heartbeats, and respiration activities. To increase the object tracking resolution, an algorithm using adaptive and digitalized post-calibration techniques were proposed and tested. Both the radiated and reflected analog pulses were converted to digital signals by a low-noise comparator and its buffer for the time interval and distance detection. Moreover, the dynamic and calibrated tracking mechanism was incorporated into the algorithm for improving tracking accuracy and to continuously localize an object’s vibration. The measured performance revealed that the proposed method successfully achieved m movement precision, thus providing its excellent tracking ability. We performed three different experimental tests to demonstrate the UWB radar’s viability. First, the radar was employed to detect the undesired structural vibration of a delta robotic arm, which was subjected to high acceleration and structural flexibility. The measured results indicated that the accuracy of 100 m can be achieved at a 10 Hz vibration frequency. Next, the apnea alarm was tested, which included an embedded sensitive sensor in the smart mattress in order to execute long-term physiological monitoring. The apnea was mitigated by auto-adjusting the patient’s sleeping posture on the mattress when the UWB sensor detected the apnea condition. The final experiment using the radar sensor was to monitor human vital signs, such as heart and breathing rates, simultaneously. In comparison with a commercial electrocardiography (ECG) instrument, this radar sensor measured 73.2 beats per minutes (bpm) with only 1.2% deviation. Our results showed that the contactless UWB sensor performs superbly and is well-suited for monitoring physiological parameters.
Wu, Wan-Chen, and 吳婉禎. "Phosphorylation of Triple Gene Block Protein 1 (TGBp1) of Bamboo Mosaic Virus Affects Viral Accumulation and Cell-to-Cell Movement in Nicotiana Benthamiana." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/ne8zq7.
Full textWang, Zheng-Cheng, and 王政承. "The replicase’s helicase-like domain of Foxtail mosaic virus interacts with the viral coat protein and this interaction affects the virus cell-to-cell movement." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/94403726090277144580.
Full text國立中興大學
生物科技學研究所
100
Foxtail mosaic virus, a member of potexvirus genus, has a single-strand RNA genome which consists of five open reading frames (ORFs). ORF1 encodes a ~152 kDa replicase responsible for replication, transcription and 5’ cap formation, while ORF5 encodes a ~ 24 kDa viral coat protein (CP) responsible for genome encapsidation and participating into viral movement within host. Previous studies on Bamboo mosaic virus (BaMV), also a potexvirus, found that there is an interaction between the replicase’s helicase-like domain (HLD) and CP, and weakness of the interaction, through mutation at A209 and N210 of CP, limits the spread out of the virus within Nicotiana benthamiana and Chenopodium quinoa, implicating that replicase is an essential component of the ribonucleoprotein complex passing through plasmodesmata. To explore whether this is a general event in potexviruses, I repeated similar experiments on FoMV in this study. The interaction between replicase’s HLD and CP of FoMV was confirmed by pull down assay. The mutations at the corresponding position on CP of FoMV (A230G and A231G) were generated on a CP-expression plasmid and infectious clones (pCF and pCFG). Pull down assay showed that the mutant coat proteins have weaker interaction with the HLD. Inoculation of N. benthamiana and C. quinoa with the infectious clones found that A230G and A231G mutations reduced the viral movement in both hosts. By trasformating protoplast, the mutaions had no influence on replication in plant cell. Based on the results, we speculate that the viral RNA of potexviruses is transported cell to cell along with the replicase to enable immediate replication once deposited in the adjacent cell.
Wang, Lan-Hui, and 王蘭蕙. "The Effect of Phosphorylation of Triple Gene Block Protein 1 (TGBp1) on Viral Replication and Cell-to-Cell Movement of Bamboo Mosaic Virus in Nicotiana benthamiana." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/90265907350259181577.
Full text慈濟大學
生命科學系碩士班
102
Bamboo mosaic virus (BaMV) belongs to the genus Potexvirus which contains five open reading frames (ORFs). Three of the five ORFs are triple gene block protein 1, 2, 3 (TGBp1, 2, 3) which involved in the viral movement between plant cells. To investigate the effect of phosphorylation of BaMV TGBp1 on cell-to-cell movement in Nicotiana benthamiana, we first used NetPhos 2.0 to predict the potential phosphorylation positions and BaMV infectious clone which expresses GFP was used to create the TGBp1 mutants. Threonine-58 and Threonine-111 were changed into Aspartate (T58D and T111D, respectively) or Alanine (T58A and T111A), and Serine-15, 18, and 247 were changed into Aspartate (S15D, S18D and S247D). In Planta experiments, we transfected the plasmid which contains wild type or mutant TGBp1 sequences into Nicotiana benthamiana leaves. By measuring fluorescent area, mutants S15D and S18D as well as T58D and T111D with mimicking constant phosphorylation or T111A which cannot be phosphorylated have reduced GFP foci area and intensity. I further studied whether these TGBp1 mutants influence BaMV replication by protoplast assays. By measuring the coat protein accumulation, S15D was significantly reduced comparing to the wild type, whereas which of T111D was not. These results indicate that mimicking constant phosphorylation on Serine-15 affects replication of BaMV and constant phosphorylation on Threonine-111 dramatically reduces the cell-to-cell movement of BaMV since its replication efficiency in the protoplasts was not affected. In summary, this study resolves the phosphorylation status on several amino acids of BaMV TGBp1 which play important roles for BaMV replication and cell-to-cell movement in N. benthamiana.