Academic literature on the topic 'Vernalization'

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Journal articles on the topic "Vernalization"

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Woods, Daniel P., Thomas S. Ream, Frédéric Bouché, Joohyun Lee, Nicholas Thrower, Curtis Wilkerson, and Richard M. Amasino. "Establishment of a vernalization requirement in Brachypodium distachyon requires REPRESSOR OF VERNALIZATION1." Proceedings of the National Academy of Sciences 114, no. 25 (June 5, 2017): 6623–28. http://dx.doi.org/10.1073/pnas.1700536114.

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A requirement for vernalization, the process by which prolonged cold exposure provides competence to flower, is an important adaptation to temperate climates that ensures flowering does not occur before the onset of winter. In temperate grasses, vernalization results in the up-regulation of VERNALIZATION1 (VRN1) to establish competence to flower; however, little is known about the mechanism underlying repression of VRN1 in the fall season, which is necessary to establish a vernalization requirement. Here, we report that a plant-specific gene containing a bromo-adjacent homology and transcriptional elongation factor S-II domain, which we named REPRESSOR OF VERNALIZATION1 (RVR1), represses VRN1 before vernalization in Brachypodium distachyon. That RVR1 is upstream of VRN1 is supported by the observations that VRN1 is precociously elevated in an rvr1 mutant, resulting in rapid flowering without cold exposure, and the rapid-flowering rvr1 phenotype is dependent on VRN1. The precocious VRN1 expression in rvr1 is associated with reduced levels of the repressive chromatin modification H3K27me3 at VRN1, which is similar to the reduced VRN1 H3K27me3 in vernalized plants. Furthermore, the transcriptome of vernalized wild-type plants overlaps with that of nonvernalized rvr1 plants, indicating loss of rvr1 is similar to the vernalized state at a molecular level. However, loss of rvr1 results in more differentially expressed genes than does vernalization, indicating that RVR1 may be involved in processes other than vernalization despite a lack of any obvious pleiotropy in the rvr1 mutant. This study provides an example of a role for this class of plant-specific genes.
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Trevaskis, Ben. "The central role of the VERNALIZATION1 gene in the vernalization response of cereals." Functional Plant Biology 37, no. 6 (2010): 479. http://dx.doi.org/10.1071/fp10056.

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Many varieties of wheat (Triticum spp.) and barley (Hordeum vulgare L.) require prolonged exposure to cold during winter in order to flower (vernalization). In these cereals, vernalization-induced flowering is controlled by the VERNALIZATION1 (VRN1) gene. VRN1 is a promoter of flowering that is activated by low temperatures. VRN1 transcript levels increase gradually during vernalization, with longer cold treatments inducing higher expression levels. Elevated VRN1 expression is maintained in the shoot apex and leaves after vernalization, and the level of VRN1 expression in these organs determines how rapidly vernalized plants flower. Some alleles of VRN1 are expressed without vernalization due to deletions or insertions within the promoter or first intron of the VRN1 gene. Varieties of wheat and barley with these alleles flower without vernalization and are grown where vernalization does not occur. The first intron of the VRN1 locus has histone modifications typically associated with the maintenance of an inactive chromatin state, suggesting this region is targeted by epigenetic mechanisms that contribute to repression of VRN1 before winter. Other mechanisms are likely to act elsewhere in the VRN1 gene to mediate low-temperature induction. This review examines how understanding the mechanisms that regulate VRN1 provides insights into the biology of vernalization-induced flowering in cereals and how this will contribute to future cereal breeding strategies.
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Zhang, HongWei, Bo Jiao, FuShuang Dong, XinXia Liang, Shuo Zhou, and HaiBo Wang. "Genome-wide identification of CCT genes in wheat (Triticum aestivum L.) and their expression analysis during vernalization." PLOS ONE 17, no. 1 (January 5, 2022): e0262147. http://dx.doi.org/10.1371/journal.pone.0262147.

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Numerous CCT genes are known to regulate various biological processes, such as circadian rhythm regulation, flowering, light signaling, plant development, and stress resistance. The CCT gene family has been characterized in many plants but remains unknown in the major cereal wheat (Triticum aestivum L.). Extended exposure to low temperature (vernalization) is necessary for winter wheat to flower successfully. VERNALIZATION2 (VRN2), a specific CCT-containing gene, has been proved to be strongly associated with vernalization in winter wheat. Mutation of all VRN2 copies in three subgenomes results in the eliminated demands of low temperature in flowering. However, no other CCT genes have been reported to be associated with vernalization to date. The present study screened CCT genes in the whole wheat genome, and preliminarily identified the vernalization related CCT genes through expression analysis. 127 CCT genes were identified in three subgenomes of common wheat through a hidden Markov model-based method. Based on multiple alignment, these genes were grouped into 40 gene clusters, including the duplicated gene clusters TaCMF6 and TaCMF8, each tandemly arranged near the telomere. The phylogenetic analysis classified these genes into eight groups. The transcriptome analysis using leaf tissues collected before, during, and after vernalization revealed 49 upregulated and 31 downregulated CCT genes during vernalization, further validated by quantitative real-time PCR. Among the differentially expressed and well-investigated CCT gene clusters analyzed in this study, TaCMF11, TaCO18, TaPRR95, TaCMF6, and TaCO16 were induced during vernalization but decreased immediately after vernalization, while TaCO1, TaCO15, TaCO2, TaCMF8, and TaPPD1 were stably suppressed during and after vernalization. These data imply that some vernalization related CCT genes other than VRN2 may exist in wheat. This study improves our understanding of CCT genes and provides a foundation for further research on CCT genes related to vernalization in wheat.
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Hong, Joon Ki, Eun Jung Suh, Sang Ryeol Park, Jihee Park, and Yeon-Hee Lee. "Multiplex CRISPR/Cas9 Mutagenesis of BrVRN1 Delays Flowering Time in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)." Agriculture 11, no. 12 (December 17, 2021): 1286. http://dx.doi.org/10.3390/agriculture11121286.

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The VERNALIZATION1 (VRN1) gene is a crucial transcriptional repressor involved in triggering the transition to flowering in response to prolonged cold. To develop Chinese cabbage (Brassica rapa L. ssp. pekinensis) plants with delayed flowering time, we designed a multiplex CRISPR/Cas9 platform that allows the co-expression of four sgRNAs targeting different regions of the endogenous BrVRN1 gene delivered via a single binary vector built using the Golden Gate cloning system. DNA sequencing analysis revealed site-directed mutations at two target sites: gRNA1 and gRNA2. T1 mutant plants with a 1-bp insertion in BrVRN1 exhibited late flowering after the vernalization. Additionally, we identified ‘transgene-free’ BrVRN1 mutant plants without any transgenic elements from the GE1 (gene-editing 1) and GE2 generations. All GE2 mutant plants contained successful edits in two out of three BrVRN1 orthologs and displayed delayed flowering time. In GE2 mutant plants, the floral repressor gene FLC1 was expressed during vernalization; but the floral integrator gene FT was not expressed after vernalization. Taken together, our data indicate that the BrVRN1 genes act as negative regulators of FLC1 expression during vernalization in Chinese cabbage, raising the possibility that the ‘transgene-free’ mutants of BrVRN1 developed in this study may serve as useful genetic resources for crop improvement with respect to flowering time regulation.
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Finnegan, E. Jean. "Vernalization." Current Biology 22, no. 12 (June 2012): R471—R472. http://dx.doi.org/10.1016/j.cub.2012.05.007.

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Rohwer, Charles L., and Royal D. Heins. "Daily Light Integral, Prevernalization Photoperiod, and Vernalization Temperature and Duration Control Flowering of Easter Cactus." HortScience 42, no. 7 (December 2007): 1596–604. http://dx.doi.org/10.21273/hortsci.42.7.1596.

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Experiments were performed on Hatiora gaertneri (Regel) Barthlott ‘Jan’ and ‘Rood’ and H. ×graeseri (Wedermann) Barthlott ‘Evita’ to determine their flowering responses to 1) daily light integral (DLI) before and during vernalization; 2) 0 to 6 weeks of short-day (SD) or long-day (LD) photoperiods before vernalization at 10, 12.5, or 15 °C; 3) propagation from April to July; 4) timing of leveling before or during inductive treatments; and 5) SD photoperiods before vernalization under darkness at 0 to 10 °C. ‘Jan’ grown under elevated DLI before vernalization and low DLI during vernalization flowered more prolifically than plants grown under low DLI before vernalization or high DLI during vernalization at 15 °C. Six weeks of SD photoperiods before vernalization increased the number of buds per flowering phylloclade after vernalization at 10 °C and increased flowering uniformity when vernalization duration was insufficient at 10 °C or vernalization temperature was 12.5 or 15 °C. For plants flowering in January, propagation the previous April produced better flowering than propagation in May, June, or July. Removal of apical phylloclades during prevernalization SD or during vernalization was deleterious to flowering. Vernalization in the dark produced marginal flowering, but SD treatment before vernalization increased the percentage of apical phylloclades flowering, buds per flowering apical phylloclade, and percentage of plants flowering after dark vernalization. ‘Evita’ flowered more poorly than either ‘Jan’ or ‘Rood’. Collectively, the most uniform flowering in January occurred when plants were exposed to a sequence of 4 to 6 weeks of SD, vernalization at 7.5 to 15 °C for 8 weeks, then growth under LD for 7 weeks.
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Landers, KF. "Vernalization responses in narrow-leafed lupin (Lupinus angustifolius) genotypes." Australian Journal of Agricultural Research 46, no. 5 (1995): 1011. http://dx.doi.org/10.1071/ar9951011.

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Three experiments were conducted to characterize vernalization response in 13 diverse narrowleafed lupin (Lupinus angustifolius) genotypes, and to identify the genetic basis of differences in vernalization response. The aim was to better understand how flowering time may be manipulated in lupin breeding. The genotypes consisted of breeding lines with parents of wild origin, plus selected commercial varieties. Treatments included response to different periods of vernalization and response to different sowing dates. Most of the genotypes required vernalization for flowering. There were three types of response to vernalization observed; an absolute requirement, a reduced response, in which vernalization did not appear to be essential for flowering, and no response in lines carrying the natural mutant gene Ku (Gladstones and Hill 1969). In genotypes with an absolute requirement for vernalization, the period of vernalization at 5�C required to ensure flowering varied between 2 and 4 weeks, and flowering was hastened by increasing periods of vernalization. When vernalization was marginally inadequate, abnormal inflorescences formed. An apparent thermosensitive response, in which vernalization hastened flowering but did not appear to be essential, occurred in cv. Wandoo, which carries the gene �efl�. This response could also possibly be explained not by the lack of an essential requirement for vernalization, but by an ability of the cultivar to respond to vernalization at fairly high temperatures, around 16�C. Crossing studies identified a major gene the same as or allelic to �efl� in one genotype, but no other single genes with major effect on vernalization response were detected in genotypes of wild origin.
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Košner, J., and K. Pánková. "Vernalization Response of Some Winter Wheat Cultivars (Triticum aestivum L.)." Czech Journal of Genetics and Plant Breeding 38, No. 3-4 (August 1, 2012): 97–103. http://dx.doi.org/10.17221/6242-cjgpb.

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For 17 cultivars of winter wheat (Triticum aestivum L.) different vernalization and photoperiod responses were detected. The effect of photoperiod sensitivity was not significantly changed by vernalization; different vernalization responses were probably due to the presence of multiple alleles at Vrn loci. The delay in heading depended on the vernalization deficit exponentially: y = Parameter (1) + (y0 – Parameter (1)) × EXP (Parameter (2) × (x – x0)). The dependence was shown to be general and significant for the given model in all the studied cultivars. Individual regressions characterised responses of cultivars to a deficit of vernalization treatment. Cluster analysis according to the characterisation obtained (full vernalization requirement, minimum vernalization requirement, insufficient vernalization and parameters of the dependence) showed the relationships between cultivars and enabled their grouping by similar profiles of vernalization, and, possibly, of photoperiod response. In individual cultivars, an attempt was made to use the model to predict performance for some agronomic traits.
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White, Scott N., Nathan S. Boyd, Rene C. Van Acker, and Clarence J. Swanton. "Studies on the flowering biology of red sorrel (Rumex acetosella) ramets from lowbush blueberry (Vaccinium angustifolium) fields in Nova Scotia, Canada." Botany 93, no. 1 (January 2015): 41–46. http://dx.doi.org/10.1139/cjb-2014-0123.

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Red sorrel (Rumex acetosella L.) is a ramet-producing herbaceous creeping perennial species commonly found as a weed in commercially managed lowbush blueberry (Vaccinium angustifolium Aiton) fields in Nova Scotia, Canada. Flowering and seed production occur primarily in overwintering ramets of this species, indicating a potential vernalization requirement for flowering. This study was therefore initiated to examine the role of vernalization, photoperiod, and pre-vernalization stimulus on ramet flowering. Red sorrel ramets propagated from creeping roots and seeds collected from established red sorrel populations in lowbush blueberry had an obligate requirement for vernalization to flower. Ramet populations maintained under pre- and post-vernalization photoperiods of 16 h flowered following 12 weeks of vernalization at 4 ± 0.1 °C, whereas those maintained under constant 16, 14, or 8 h photoperiods without vernalization did not flower. Vernalization for 10 weeks maximized, but did not saturate, the flowering response. Pre-vernalization photoperiod affected flowering response, with increased flowering frequency observed in ramet populations exposed to decreasing, rather than constant, photoperiod prior to vernalization. This study represents the first attempt to determine the combined effects of vernalization and photoperiod on red sorrel flowering, and the results provide a benchmark for the future study of flowering and sexual reproduction in this economically important perennial weed species.
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Terzioğlu, Serpil. "Responses of Some Turkish Wheat Cultivars to Vernalization and Photoperiod." Experimental Agriculture 24, no. 2 (April 1988): 237–45. http://dx.doi.org/10.1017/s0014479700015982.

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SUMMARYThe vernalization and photoperiodic response of six locally adapted bread wheat cultivars grown under natural daylength conditions during the summer or winter months was examined in glasshouse experiments. The wheat was vernalized by chilling imbibed grains at 2 ± 1°C for 0, 15 or 45 days. Vernalization for 45 days followed by long summer days led to floral initiation in all cultivars within 28 days but vernalization for 0 or 15 days only led to floral initiation in one cultivar. Vernalization followed by long days reduced the time from transplanting to anthesis, resulting in early ear emergence. Vernalization followed by short days accelerated the development of all the cultivars, but normal development could also occur without vernalization at this time of year. Apical differentiation of the primary shoot and its length and development gave the most reliable information on the period of vernalization required.
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Dissertations / Theses on the topic "Vernalization"

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Zanewich, Karen P., and University of Lethbridge Faculty of Arts and Science. "Vernalization and gibberellin physiology of winter canola." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1993, 1993. http://hdl.handle.net/10133/52.

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Winter canola (Brassica napus cv. Crystal) requires vernalization, exposure to chilling, to induce bolting and flowering. Since gibberellins (GAs) have been implicated in the regulation of stem elongation and reproductive development in numerous plants, the role of GAs in events induced by vernalization was investigated. Three classical approaches for studying GA physiology were taken. Plant growth regulators were applied and showed that: (i) GA application induced stem elongation but not flowering in nonvernalized plants and (ii) plant growth retardants that block GA biosynthesis prevented elongation and flowering in vernalized plants. Endogenous GAs were extracted from vernalized and nonvernalized shoot tips, chromatographically purified and quantified by gas chromatography-selected ion monitoring. GA1,3,8,19 and 20 concentrations were higher in the vernalized shoots following vernalization. Feeds of [3H]GA20 to vernalized and nonvernalized plants demonstrated higher rates of [3H]GA1 formation after vernalization, suggesting increased metabolism to the biologically active form. Collectively, these studies indicate a regulatory role of GAs in the control of stem elongation in winter canola, but the role of GAs in flowering was less clear. Vernalization apparently induces stem elongation by increasing GA synthesis and particularly the biosynthesis of GA1.
xii, 138 leaves : ill., ports. ; 28 cm.
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Geraldo, Nuno D. C. "Molecular changes underlying the early stages of vernalization." Thesis, University of East Anglia, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433587.

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Murphy, Lee Anne. "Vernalization response in spring oilseed rape, Brassica napus L." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq23642.pdf.

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Strange, Amy. "Natural Variation in the Vernalization Response of Arabidopsis thaliana." Thesis, University of East Anglia, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502370.

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Within Arabidopsis thaliana there is extensive natural variation in the timing of flowering. This thesis focuses on the variation in vernalization response, manifested as a requirement for different lengths of cold in order to fully accelerate flowering. During vernalization, the gene encoding the floral repressor FLC is silenced and this is maintained during subsequent development by a Polycomb-mediated chromatin silencing mechanism. Three accessions from Sweden (Lov-I, Ull-2-5 and Var-2-6) require extended vernalization due to a slower accumulation ofthe chromatin silencing during the cold. In this study a QTL analysis mapped the variation in vernalization response to chromosomes 1,4 and 5. Further fine mapping identified FLC as one ofthe loci underlying the QTL and polymorphisms in FLC were located in putative regulatory rather than protein-coding regions. Allelic variation in FLC was found to be directly responsible for variation in the stability ofFLC repression after short lengths of vernalization. Work is ongoing to map the nucleotide polymorphisms which are directly responsible for the phenotypic variation. The vernalization response of two accessions from America (Kno-I8 and RRS-IO) was also investigated. They express FLC at extremely low levels, but are late flowering. Vernalization response QTL in these accessions again mapped to chromosomes 1,4 and 5. Low FLC expression was associated with a transposable element insertion in intron 1 ofFLC. Cloning of this transposon into a wild-type FLC allele showed it inactivates function and demonstrated modifiers in the American accessions that result in their late flowering. Initial results are described from a European common garden experiment, addressing whether flowering time is an adaptive trait. It was found that the Swedish accessions described above have low fitness in non-vernalizing conditions.
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Williams, C. A. "Relationships between leaf development, carbohydrates and vernalization in cauliflower." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383746.

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Barrett, Lynne. "The role of Arabidopsis VRN1 in mediating the vernalization response." Thesis, University of East Anglia, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423800.

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Mandel, Roger M. "Hormones, photoperiod and vernalization in the control of flowering in Brassica." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/nq20752.pdf.

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Duncan, Susan. "Predicting the impact of climate change on vernalization for Arabidopsis thaliana." Thesis, University of East Anglia, 2015. https://ueaeprints.uea.ac.uk/54405/.

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Winter annual Arabidopsis thaliana plants require a prolonged period of cold, known as vernalization, to ensure prompt floral transition occurs in spring. This thesis addresses the question of whether partial saturation of cold requirements might delay flowering under future climate scenarios. Laboratory experiments set up to parameterize a predictive model revealed a surprising optimal vernalizing temperature for the Swedish accession Lov-1. Field experiments in Northern Sweden support the theory that this optimum likely reflects adaptation to autumn, rather than winter temperatures. A chilling unit model incorporating empirically derived parameters forecast an overall increase in effective vernalizing days for A. thaliana in northern Sweden. This increase is the result of an overall reduction in sub-zero temperatures that are predicted for northerly latitudes by the end of the century. Reductions in the number of effective vernalizing days were predicted for England and Spain, however these are unlikely to counteract the forcing effects of increased spring temperatures at these locations. This thesis also presents a novel method that enables single RNA molecules to be visualized for the first time in plants. This method was used to determine cell-to-cell variation and subcellular distribution of key vernalization gene transcripts before, during and after cold exposure. These results provide a unique insight into how plants perceive and integrate longterm temperature cues at the cellular level In summary, this thesis predicts the potential impact of climate change on A. thaliana vernalization across its species’ range. It also dissects transcriptional mechanisms that underlie long-term temperature integration. Modulation of these mechanisms is likely to be key for survival of some wild species and for maximizing crop yields under future climate scenarios.
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McKeown, Meghan. "Evolution of Vernalization and Photoperiod-Regulated Genetic Networks in the Grass Subfamily Pooideae." ScholarWorks @ UVM, 2016. http://scholarworks.uvm.edu/graddis/650.

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Flowering time is a carefully regulated trait that integrates cues from temperature and photoperiod to coordinate flowering at favorable times of the year. This dissertation aims to understand the evolution of genetic architecture that facilitated the transition of Pooideae, a subfamily of grass, from the tropics to the temperate northern hemisphere approximately 50 million years ago. Two traits hypothesized to have facilitated this evolutionary shift are the use of long-term low-temperature (vernalization) to ready plants for flowering, and long-day photoperiods to induce flowering. In chapter one I review literature on the regulation of grass flowering by vernalization and photoperiod, and in chapters two and three I determine the role of VERNALIZATION 1 (VRN1) and VRN2, known to confer vernalization responsiveness in core Pooideae crop species, in flowering time across Pooideae. In chapter four, I then test predictions of the hypothesis that the Brachypodium distachyon miR5200 ortholog in the ancestor of Pooideae was important for suppressing short day flowering through its negative regulation of flowering time integrator FLOWERING LOCUS T (FT)/VERNALIZATION3 (VRN3). In combination with other studies, my data demonstrate that VRN1-mediated vernalization responsiveness evolved early in the Pooideae, while VRN2-mediated vernalization responsiveness appears to have evolved much later in the diversification of Pooideae. Although miR5200 likely evolved early in the Pooideae, its transcriptional regulation by short day photoperiod appears derived within Brachypodium distachyon. This work answers important questions about the evolutionary origin of temperature- and photoperiod-mediated flowering in an economically important clade that contains crop species such as wheat (Triticum aestivum) and barley (Hordeum vulgare). Directions for future work on this topic are discussed in chapter 5.
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Genger, Ruth Kathleen, and Ruth Genger@csiro au. "Cytosine methylation, methyltransferases and flowering time in Arabidopsis thaliana." The Australian National University. Faculty of Science, 2000. http://thesis.anu.edu.au./public/adt-ANU20011127.115231.

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Environmental signals such as photoperiod and temperature provide plants with seasonal information, allowing them to time flowering to occur in favourable conditions. Most ecotypes of the model plant Arabidopsis thaliana flower earlier in long photoperiods and after prolonged exposure to cold (vernalization). The vernalized state is stable through mitosis, but is not transmitted to progeny, suggesting that the vernalization signal may be transmitted via a modification of DNA such as cytosine methylation. The role of methylation in the vernalization response is investigated in this thesis. ¶ Arabidopsis plants transformed with an antisense construct to the cytosine methyltransferase METI (AMT) showed significant decreases in methylation. AMT plants flowered significantly earlier than unvernalized wildtype plants, and the promotion of flowering correlated with the extent of demethylation. The flowering time of mutants with decreased DNA methylation (ddm1) was promoted only in growth conditions in which wildtype plants showed a vernalization response, suggesting that the early flowering response to demethylation operated specifically through the vernalization pathway. ¶ The AMT construct was crossed into two late flowering mutants that differed in vernalization responsiveness. Demethylation promoted flowering of the vernalization responsive mutant fca, but not of the fe mutant, which has only a slight vernalization response. This supports the hypothesis that demethylation is a step in the vernalization pathway. ¶ The role of gibberellic acid (GA) in the early flowering response to demethylation was investigated by observing the effect of the gai mutation, which disrupts the GA signal transduction pathway, on flowering time in plants with demethylated DNA. The presence of a single gai allele delayed flowering, suggesting that the early flowering response to demethylation requires a functional GA signal transduction pathway, and that demethylation increases GA levels or responses, directly or indirectly. ¶ In most transgenic lines, AMT-mediated demethylation did not fully substitute for vernalization. This indicates that part of the response is not affected by METI-mediated methylation, and may involve a second methyltransferase or a factor other than methylation. A second Arabidopsis methyltransferase, METIIa, was characterized and compared to METI. The two genes are very similar throughout the coding region, and share the location of their eleven introns, indicating that they diverged relatively recently. Both are transcribed in all tissues and at all developmental stages assayed, but the level of expression of METI is significantly higher than that of METIIa. The possible functions of METI, METIIa, and other Arabidopsis cytosine methyltransferase genes recently identified are discussed.
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Books on the topic "Vernalization"

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Hill, David E. Globe artichoke trials, 1998, 2000 management of yield using induced or natural vernalization. New Haven: Connecticut Agricultural Experiment Station, 2001.

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Hill, David E. Globe artichoke trials, 1998, 2000 management of yield using induced or natural vernalization. New Haven: Connecticut Agricultural Experiment Station, 2001.

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Mazumdar, Bibhas Chandra. Photoperiodism and Vernalization in Plants. Daya Publishing House,India, 2006.

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Baloch, Dost M. Vernalization requirement studies with Pacific Northwest wheats. 1994.

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Amasino, Richard, Elizabeth Dennis, Caroline Dean, Joanna Putterill, and Christian Jung, eds. Vernalization and Flowering Time: Celebrating 20 Years of FLC. Frontiers Media SA, 2021. http://dx.doi.org/10.3389/978-2-88971-739-2.

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Rojas-Calvo, Carlos Enrique. Effect of seed burial and vernalization on germination and growth of Bromus carinatus and its control with several herbicides. 1990.

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Pan, Aihong. Genetic analysis of vernalization, photoperiod, and winter hardiness in barley (Hordeum vulgare L.). 1994.

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Gonzalez, Adelmo Monsalve. Protein composition and functional properties of spring and winter wheats as affected by vernalization. 1991.

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Gleichsner, Jean Ann. Biology of Bromus rigidus: Interference in winter wheat, seed longevity in the soil, and vernalization requirements for flowering. 1988.

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Book chapters on the topic "Vernalization"

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Kim, Dong-Hwan, and Sibum Sung. "Vernalization." In Temperature and Plant Development, 79–95. Oxford: John Wiley & Sons, Inc, 2013. http://dx.doi.org/10.1002/9781118308240.ch4.

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Yan, Liuling, Genqiao Li, Ming Yu, Tilin Fang, Shuanghe Cao, and Brett F. Carver. "Genetic Mechanisms of Vernalization Requirement Duration in Winter Wheat Cultivars." In Advances in Wheat Genetics: From Genome to Field, 117–25. Tokyo: Springer Japan, 2015. http://dx.doi.org/10.1007/978-4-431-55675-6_13.

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Paro, Renato, Ueli Grossniklaus, Raffaella Santoro, and Anton Wutz. "Cellular Memory." In Introduction to Epigenetics, 49–66. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-68670-3_3.

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AbstractThe identity of cells in an organism is largely defined by their specific transcriptional profile. During cell division, these profiles need to be faithfully inherited to the daughter cells to ensure the maintenance of cell structure and function in a cell lineage. Here, you will learn how two groups of chromatin regulators, the Polycomb group (PcG) and the Trithorax group (TrxG), act in an antagonistic manner to maintain differential gene expression states. Members of the PcG cooperate in large multiprotein complexes to modify histones with repressive marks, resulting in condensed chromatin domains. Conversely, the TrxG proteins counteract the repressed domains by opening nucleosomal structures and establishing activating histone modifications. PcG and TrxG proteins are evolutionary highly conserved and control diverse processes, such as the identity of stem cells in mammalian development to the process of vernalization in plants.
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Yan, Weikai, and Donald H. Wallace. "A Physiological-Genetic Model of Photoperiod-Temperature Interactions in Photoperiodism, Vernalization, and Male Sterility of Plants." In Horticultural Reviews, 73–123. Oxford, UK: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470650585.ch3.

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Ream, T. S. "Vernalization." In Encyclopedia of Applied Plant Sciences, 473–82. Elsevier, 2017. http://dx.doi.org/10.1016/b978-0-12-394807-6.00110-6.

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KREKULE, J. "VERNALIZATION IN WHEAT." In Manipulation of Flowering, 159–69. Elsevier, 1987. http://dx.doi.org/10.1016/b978-0-407-00570-9.50017-x.

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Piñeiro, M., and J. M. Martínez-Zapater. "REGULATORS OF GROWTH | Vernalization." In Encyclopedia of Applied Plant Sciences, 1056–63. Elsevier, 2003. http://dx.doi.org/10.1016/b0-12-227050-9/00074-0.

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DALE, P. J., K. ARUMUGANATHAN, S. J. DALTON, and J. P. COOPER. "VERNALIZATION STUDIES IN LOLIUM." In Plant Tissue Culture and its Agricultural Applications, 63–68. Elsevier, 1986. http://dx.doi.org/10.1016/b978-0-407-00921-9.50011-7.

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NAPP-ZINN, K. "VERNALIZATION—ENVIRONMENTAL AND GENETIC REGULATION." In Manipulation of Flowering, 123–32. Elsevier, 1987. http://dx.doi.org/10.1016/b978-0-407-00570-9.50014-4.

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He, Xin. "An Insight into the Responses of Early-Maturing Brassica napus to Different Low-Temperature Stresses." In Abiotic Stress in Plants [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.93708.

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Rapeseed (Brassica napus L.) is an important oil crop worldwide, responds to vernalization, and shows an excellent tolerance to cold stresses during vegetative stage. The winter-type and semi-winter-type rapeseed were typical winter biennial plants in Europe and China. In recent years, more and more early-maturing semi-winter rapeseed varieties were planted across China. Unfortunately, the early-maturing rapeseed varieties with low cold tolerance have higher risk of freeze injury in cold winter and spring. The molecular mechanisms for coping with different low-temperature stress conditions in rapeseed recently had gained more attention and development. The present review gives an insight into the responses of early-maturing B. napus to different low-temperature stresses (chilling, freezing, cold-acclimation, and vernalization), and the strategies to improve tolerance against low-temperature stresses are also discussed.
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Conference papers on the topic "Vernalization"

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"What we know about vernalization process in wheat." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-154.

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"Modelling the dynamics of Vernalization: The role of conceptualization in model formulation." In 21st International Congress on Modelling and Simulation (MODSIM2015). Modelling and Simulation Society of Australia and New Zealand, 2015. http://dx.doi.org/10.36334/modsim.2015.b6.anderssen.

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Neubauer, McKayla. "Vernalization and cold acclimation of two divergent Camelina sativa biotypes: An RNAseq time course study." In ASPB PLANT BIOLOGY 2020. USA: ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1007241.

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Zubanova, Yu S., V. A. Filobok, E. A. Guenkova, E. R. Davoyan, D. M. Boldakov, and D. S. Mikov. "Identification of allelic combinations of the Ppd-D1, Vrn-A1, Vrn-B1 and Vrn-D1 genes in common wheat lines obtained in the National Center of Grain named after P. P. Lukyanenko." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-63.

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An analysis of the allelic composition of the genes determining photoperiodic sensitivity (Ppd-D1) and the need for vernalization (Vrn-A1, Vrn-B1, Vrn-D1) was carried out in 286 common wheat lines obtained in the National Center of Grain named after P. P. Lukyanenko with the use of allele-specific primers. The analyzed samples were distributed over 21 haplotypes; the dominant allele of the Ppd-D1a gene prevailed in the studied material. 123 lines of common wheat carry a combination of D-RRD alleles. The lines that can be attributed to the group of alternate wheat (R-RDR, R-RRD) were identified. All studied samples carry the recessive allele of at least one VRN1 gene.
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Xianyun Du, Jihong Cheng, Hui Lei, and Shaohui Wang. "Effects of vernalization on carbohydrate content in leaves and flashy taproot of radish (Raphanus sativus L.) during flashy taproot development." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5966119.

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LELIŪNIENĖ, Jolanta, Ligita BALEŽENTIENĖ, and Evaldas KLIMAS. "FESTULOLIUM METABOLITES ACCUMULATION RESPONSE TO PHOTOPERIOD OF FLOWERING TERMOINDUCTION." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.003.

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Most of plant development, physiological and metabolic processes are regulated by not only soluble sugars such as glucose and sucrose, but also by other signal molecules, such as phytohormones. The investigation of flowering induction, considering the influence of vernalisation duration and photoperiod on morphogenesis stages and accumulation metabolites in the new Festulolium cultivars ’Vėtra’ and ’Punia’ was carried out at the phytotron complex of the Plant Physiology Laboratory, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry in 2011-2012. The data revealed impact of vernalisation and photoperiod on accumulation of both types of assessed metabolies, i.e. phytohormones and saccharides, and thus confirmed their substantial role. 90 short-day vernalisation induced the highest total phytohormone content in ‘Vėtra’, when plant achieved tillering stage and was going for intensive growth when growth regulators will be important in the metabolic regulation. The highest phytohormone content was recorded after long – day 130+20 day vernalization at VII and IV organogenesis stages of ‘Vėtra’ and ʽPuniaʼ respectively. Saccharides content significantly depended on photoperiod and temperature during vernalisation and was different in ’Vėtra’ and ’Punia’.
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KLIMAS, Evaldas, Jolanta LELIŪNIENĖ, and Ligita BALEŽENTIENĖ. "VERNALISATION IMPACT ON BIOMETRICAL PARAMETERS OF FESTULOLIUM VARIETIES." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.002.

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Many plants, including Festulolium, grown in temperate climates require vernalization and must experience a period of low winter temperature to initiate or accelerate the flowering process. The aim of research was to investigate impact of vernalisation thermoinduction on growth and development parameters of Festulolium varieties ‘Vėtra’ and ‘Punia DS’. Investigations were carried out in Lithuanian Research Centre for Agriculture and Forestry Institute of Horticulture, Plant Physiology Laboratory of phytotron complex in 2011–2012. Some peculiarities of growth and development of. Festulolium varieties ’Vėtra’ and ‘Punia DS’ were investigated. 5 plants were sown in each 5 litre pot in neutral peat substrate (pH 6–6.5). The plants were grown in greenhouse till the tillering phase at the temperature of 20±2 °C at daytime and 16±2 °C at night. Later plants were moved to low temperature chambers for 90, 110 and 130 days for passing of vernalisation processes, where the 8 and 16 hour photoperiod were maintained at 4 °C temperature. After vernalisation periods plants were removed to a greenhouse for additional 20 days. Biometric parameters, namely plant height, shoot number and dry mass were measured after each period in greenhouse and climatic chambers. The data revealed different response of Festulolium varieties ‘Vėtra’ and ‘Punia DS’ to vernalisation conditions. According to our data ‘Vėtra’ plant height was 6 % higher than the ‘Punia DS’ after 130+20 days of vernalisation. Nonetheless, vernalisation temperature conditions have no significant impact on shoot number. 110 and 130 long-day photoperiod significantly impacted on shoot number of Festulolium ʽVėtraʼ. Otherwise, 90 days vernalisation of both photoperiod induced significantly the highest length of ‘Punia DSʼ shoots. ‘Vėtraʼ accumulated significantly the maximum dry matter after 110 days vernalisation period, than that after 90 and 130 days.
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Reports on the topic "Vernalization"

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Abbo, Shahal, Hongbin Zhang, Clarice Coyne, Amir Sherman, Dan Shtienberg, and George J. Vandemark. Winter chickpea; towards a new winter pulse for the semiarid Pacific Northwest and wider adaptation in the Mediterranean basin. United States Department of Agriculture, January 2011. http://dx.doi.org/10.32747/2011.7597909.bard.

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Original objectives: [a] Screen an array of chickpea and wild annual Cicer germplasm for winter survival. [b] Genetic analysis of winter hardiness in domesticated x wild chickpea crosses. [c] Genetic analysis of vernalization response in domesticated x wild chickpea crosses. [d] Digital expression analysis of a core selection of breeding and germplasm lines of chickpea that differ in winter hardiness and vernalization. [e] Identification of the genes involved in the chickpea winter hardiness and vernalization and construction of gene network controlling these traits. [f] Assessing the phenotypic and genetic correlations between winter hardiness, vernalization response and Ascochyta blight response in chickpea. The complexity of the vernalization response and the inefficiency of our selection experiments (below) required quitting the work on ascochyta response in the framework of this project. Background to the subject: Since its introduction to the Palouse region of WA and Idaho, and the northern Great Plains, chickpea has been a spring rotation legume due to lack of winter hardiness. The short growing season of spring chickpea limits its grain yield and leaves relatively little stubble residue for combating soil erosion. In Israel, chilling temperatures limit pod setting in early springs and narrow the effective reproductive time window of the crop. Winter hardiness and vernalization response of chickpea alleles were lost due to a series of evolutionary bottlenecks; however, such alleles are prevalent in its wild progenitor’s genepool. Major conclusions, solutions, achievements: It appears that both vernalization response and winter hardiness are polygenic traits in the wild-domesticated chickpea genepool. The main conclusion from the fieldwork in Israel is that selection of domesticated winter hardy and vernalization responsive types should be conducted in late flowering and late maturity backgrounds to minimize interference by daylength and temperature response alleles (see our Plant Breeding paper on the subject). The main conclusion from the US winter-hardiness studies is that excellent lines have been identified for germplasm release and continued genetic study. Several of the lines have good seed size and growth habit that will be useful for introgressing winter-hardiness into current chickpea cultivars to develop releases for autumn sowing. We sequenced the transcriptomes and profiled the expression of genes in 87 samples. Differential expression analysis identified a total of 2,452 differentially expressed genes (DEGs) between vernalized plants and control plants, of which 287 were shared between two or more Cicer species studied. We cloned 498 genes controlling vernalization, named CVRN genes. Each of the CVRN genes contributes to flowering date advance (FDA) by 3.85% - 10.71%, but 413 (83%) other genes had negative effects on FDA, while only 83 (17%) had positive effects on FDA, when the plant is exposed to cold temperature. The cloned CVRN genes provide new toolkits and knowledge to develop chickpea cultivars that are suitable for autumn-sowing. Scientific & agricultural implications: Unlike the winter cereals (barley, wheat) or pea, in which a single allelic change may induce a switch from winter to spring habit, we were unable to find any evidence for such major gene action in chickpea. In agricultural terms this means that an alternative strategy must be employed in order to isolate late flowering – ascochyta resistant (winter types) domesticated forms to enable autumn sowing of chickpea in the US Great Plains. An environment was identified in U.S. (eastern Washington) where autumn-sown chickpea production is possible using the levels of winter-hardiness discovered once backcrossed into advanced cultivated material with acceptable agronomic traits. The cloned CVRN genes and identified gene networks significantly advance our understanding of molecular mechanisms underlying plant vernalization in general, and chickpea in particular, and provide a new toolkit for switching chickpea from a spring-sowing to autumn-sowing crop.
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Samach, Alon, Douglas Cook, and Jaime Kigel. Molecular mechanisms of plant reproductive adaptation to aridity gradients. United States Department of Agriculture, January 2008. http://dx.doi.org/10.32747/2008.7696513.bard.

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Annual plants have developed a range of different mechanisms to avoid flowering (exposure of reproductive organs to the environment) under adverse environmental conditions. Seasonal environmental events such as gradual changes in day length and temperature affect the timing of transition to flowering in many annual and perennial plants. Research in Arabidopsis and additional species suggest that some environmental signals converge on transcriptional regulation of common floral integrators such as FLOWERING LOCUS T (FT). Here we studied environmental induction of flowering in the model legume Medicago truncatula. Similarly to Arabidopsis, the transition to flowering in M. truncatula is hastened by long photoperiods and long periods of vernalization (4°C for 2-3 weeks). Ecotypes collected in Israel retain a vernalization response even though winter temperatures are way above 4°C. Here we show that this species is also highly responsive (flowers earlier) to mild ambient temperatures up to 19°C simulating winter conditions in its natural habitat. Physiological experiments allowed us to time the transition to flowering due to low temperatures, and to compare it to vernalization. We have made use of natural variation, and induced mutants to identify key genes involved in this process, and we provide here data suggesting that an FT gene in M.truncatula is transcriptionally regulated by different environmental cues. Flowering time was found to be correlated with MtFTA and MtFTB expression levels. Mutation in the MtFTA gene showed a late flowering phenotype, while over-expressing MtFTA in Arabidopsis complemented the ft- phenotype. We found that combination of 4°C and 12°C resulted in a synergistic increase in MtFTB expression, while combining 4°C and long photoperiods caused a synergistic increase in MtFTA expression. These results suggest that the two vernalization temperatures work through distinct mechanisms. The early flowering kalil mutant expressed higher levels of MtFTA and not MtFTB suggesting that the KALIL protein represses MtFTA specifically. The desert ecotype Sde Boker flowers earlier in response to short treatments of 8-12oc vernalization and expresses higher levels of MtFTA. This suggests a possible mechanism this desert ecotype developed to flower as fast as possible and finish its growth cycle before the dry period. MtFTA and FT expression are induced by common environmental cues in each species, and expression is repressed under short days. Replacing FT with the MtFTA gene (including regulatory elements) caused high MtFTA expression and early flowering under short days suggesting that the mechanism used to repress flowering under short days has diversified between the two species.The circadian regulated gene, GIGANTEA (GI) encodes a unique protein in Arabidopsis that is involved in flowering mechanism. In this research we characterized how the expression of the M.truncatula GI ortholog is regulated by light and temperature in comparison to its regulation in Arabidopsis. In Arabidopsis GI was found to be involved in temperature compensation to the clock. In addition, GI was found to be involved in mediating the effect of temperature on flowering time. We tested the influence of cold temperature on the MtGI gene in M.truncatula and found correlation between MtGI levels and extended periods of 12°C treatment. MtGI elevation that was found mostly after plants were removed from the cold influence preceded the induction of MtFT expression. This data suggests that MtGI might be involved in 12°C cold perception with respect to flowering in M.truncatula. GI seems to integrate diverse environmental inputs and translates them to the proper physiological and developmental outputs, acting through several different pathways. These research enabled to correlate between temperature and circadian clock in M.truncatula and achieved a better understanding of the flowering mechanism of this species.
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Abbott, Albert G., Doron Holland, Douglas Bielenberg, and Gregory Reighard. Structural and Functional Genomic Approaches for Marking and Identifying Genes that Control Chilling Requirement in Apricot and Peach Trees. United States Department of Agriculture, September 2009. http://dx.doi.org/10.32747/2009.7591742.bard.

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Structural and functional genomic approaches for marking and identifying genes that control chilling requirement in apricot and peach trees. Specific aims: 1) Identify and characterize the genetic nature of chilling requirement for flowering and dormancy break of vegetative shoots in Prunusgermplasm through the utilization of existing apricot (NeweYa'ar Research Center, ARO) and peach (Clemson University) genetic mapping populations; 2) Use molecular genetic mapping techniques to identify markers flanking genomic regions controlling chilling; 3) Comparatively map the regions controlling chilling requirement in apricot and peach and locate important genomic regions influencing chilling requirement on the Prunus functional genomic database as an initial step for identification of candidate genes; 4) Develop from the functional genomics database a set of markers facilitating the development of cultivars with optimized chilling requirements for improved and sustained fruit production in warm-winter environments. Dormant apricot (prunus armeniaca L.) and peach [Prunus persica (L.) Batsch] trees require sustained exposure to low, near freezing, temperatures before vigorous floral and vegetative bud break is possible after the resumption of warm temperatures in the spring. The duration of chilling required (the chilling requirement, CR) is determined by the climatic adaptation of the particular cultivar, thus limiting its geographic distribution. This limitation is particularly evident when attempting to introduce superior cultivars to regions with very warm winter temperatures, such as Israel and the coastal southern United States. The physiological mechanism of CR is not understood and although breeding programs deliberately manipulate CR in apricot and peach crosses, robust closely associated markers to the trait are currently not available. We used segregating populations of apricot (100 Fl individuals, NeweYa'ar Research Center, ARO) and peach (378 F2 individuals, Clemson University) to discover several discreet genomic loci that regulate CR and blooming date. We used the extensive genomic/genetic resources available for Prunus to successfully combine our apricot and peach genetic data and identify five QTL with strong effects that are conserved between species as well as several QTL that are unique to each species. We have identified markers in the key major QTL regions for testing in breeding programs which we are carrying out currently; we have identified an initial set of candidate genes using the peach physical/transcriptome map and whole peach genome sequences and we are testing these currently to identify key target genes for manipulation in breeding programs. Our collaborative work to date has demonstrated the following: 1) CR in peach and apricot is predominantly controlled by a limited number ofQTL loci, seven detected in a peach F2 derived map comprising 65% of the character and 12 in an apricot Fl map comprising 71.6% and 55.6% of the trait in the Perfection and A. 1740 parental maps, respectively and that peach and apricot appear in our initial maps to share five genomic intervals containing potentially common QTL. 2) Application of common anchor markers of the Prunus/peach, physical/genetic map resources has allowed us not only to identify the shared intervals but also to have immediately available some putative candidate gene information from these intervals, the EVG region on LG1 in peach the TALY 1 region in apricot on LG2 in peach; and several others involved in vernalization pathways (LGI and LG7). 3) Mapped BACcontigs are easily defined from the complete physical map resources in peach through the common SSR markers that anchor our CR maps in the two species, 4) Sequences of BACs in these regions can be easily mined for additional polymorphic markers to use in MAS applications.
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