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Journal articles on the topic 'Vector viruses'
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1
Wang, Xiao-Wei, and Stéphane Blanc. "Insect Transmission of Plant Single-Stranded DNA Viruses." Annual Review of Entomology 66, no. 1 (January 7, 2021): 389–405. http://dx.doi.org/10.1146/annurev-ento-060920-094531.
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Of the approximately 1,200 plant virus species that have been described to date, nearly one-third are single-stranded DNA (ssDNA) viruses, and all are transmitted by insect vectors. However, most studies of vector transmission of plant viruses have focused on RNA viruses. All known plant ssDNA viruses belong to two economically important families, Geminiviridae and Nanoviridae, and in recent years, there have been increased efforts to understand whether they have evolved similar relationships with their respective insect vectors. This review describes the current understanding of ssDNA virus–vector interactions, including how these viruses cross insect vector cellular barriers, the responses of vectors to virus circulation, the possible existence of viral replication within insect vectors, and the three-way virus–vector–plant interactions. Despite recent breakthroughs in our understanding of these viruses, many aspects of plant ssDNA virus transmission remain elusive. More effort is needed to identify insect proteins that mediate the transmission of plant ssDNA viruses and to understand the complex virus–insect–plant three-way interactions in the field during natural infection.
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Kaur, Navneet, Daniel K. Hasegawa, Kai-Shu Ling, and William M. Wintermantel. "Application of Genomics for Understanding Plant Virus-Insect Vector Interactions and Insect Vector Control." Phytopathology® 106, no. 10 (October 2016): 1213–22. http://dx.doi.org/10.1094/phyto-02-16-0111-fi.
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The relationships between plant viruses and their vectors have evolved over the millennia, and yet, studies on viruses began <150 years ago and investigations into the virus and vector interactions even more recently. The advent of next generation sequencing, including rapid genome and transcriptome analysis, methods for evaluation of small RNAs, and the related disciplines of proteomics and metabolomics offer a significant shift in the ability to elucidate molecular mechanisms involved in virus infection and transmission by insect vectors. Genomic technologies offer an unprecedented opportunity to examine the response of insect vectors to the presence of ingested viruses through gene expression changes and altered biochemical pathways. This review focuses on the interactions between viruses and their whitefly or thrips vectors and on potential applications of genomics-driven control of the insect vectors. Recent studies have evaluated gene expression in vectors during feeding on plants infected with begomoviruses, criniviruses, and tospoviruses, which exhibit very different types of virus-vector interactions. These studies demonstrate the advantages of genomics and the potential complementary studies that rapidly advance our understanding of the biology of virus transmission by insect vectors and offer additional opportunities to design novel genetic strategies to manage insect vectors and the viruses they transmit.
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Gray, Stewart M., and Nanditta Banerjee. "Mechanisms of Arthropod Transmission of Plant and Animal Viruses." Microbiology and Molecular Biology Reviews 63, no. 1 (March 1, 1999): 128–48. http://dx.doi.org/10.1128/mmbr.63.1.128-148.1999.
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SUMMARY A majority of the plant-infecting viruses and many of the animal-infecting viruses are dependent upon arthropod vectors for transmission between hosts and/or as alternative hosts. The viruses have evolved specific associations with their vectors, and we are beginning to understand the underlying mechanisms that regulate the virus transmission process. A majority of plant viruses are carried on the cuticle lining of a vector’s mouthparts or foregut. This initially appeared to be simple mechanical contamination, but it is now known to be a biologically complex interaction between specific virus proteins and as yet unidentified vector cuticle-associated compounds. Numerous other plant viruses and the majority of animal viruses are carried within the body of the vector. These viruses have evolved specific mechanisms to enable them to be transported through multiple tissues and to evade vector defenses. In response, vector species have evolved so that not all individuals within a species are susceptible to virus infection or can serve as a competent vector. Not only are the virus components of the transmission process being identified, but also the genetic and physiological components of the vectors which determine their ability to be used successfully by the virus are being elucidated. The mechanisms of arthropod-virus associations are many and complex, but common themes are beginning to emerge which may allow the development of novel strategies to ultimately control epidemics caused by arthropod-borne viruses.
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Chare, Elizabeth R., and Edward C. Holmes. "Selection pressures in the capsid genes of plant RNA viruses reflect mode of transmission." Journal of General Virology 85, no. 10 (October 1, 2004): 3149–57. http://dx.doi.org/10.1099/vir.0.80134-0.
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To determine the selection pressures faced by RNA viruses of plants, patterns of nonsynonymous (d N) and synonymous (d S) substitution in the capsid genes of 36 viruses with differing modes of transmission were analysed. This analysis provided strong evidence that the capsid proteins of vector-borne plant viruses are subject to greater purifying selection on amino acid change than those viruses transmitted by other routes and that virus–vector interactions impose greater selective constraints than those between virus and plant host. This could be explained by specific interactions between capsid proteins and cellular receptors in the insect vectors that are necessary for successful transmission. However, contrary to initial expectations based on phylogenetic relatedness, vector-borne plant viruses are subject to weaker selective constraints than vector-borne animal viruses. The results suggest that the greater complexity involved in the transmission of circulative animal viruses compared with non-circulative plant viruses results in more intense purifying selection.
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Roberts, Anjeanette, Linda Buonocore, Ryan Price, John Forman, and John K. Rose. "Attenuated Vesicular Stomatitis Viruses as Vaccine Vectors." Journal of Virology 73, no. 5 (May 1, 1999): 3723–32. http://dx.doi.org/10.1128/jvi.73.5.3723-3732.1999.
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ABSTRACT We showed previously that a single intranasal vaccination of mice with a recombinant vesicular stomatitis virus (VSV) expressing an influenza virus hemagglutinin (HA) protein provided complete protection from lethal challenge with influenza virus (A. Roberts, E. Kretzschmar, A. S. Perkins, J. Forman, R. Price, L. Buonocore, Y. Kawaoka, and J. K. Rose, J. Virol. 72:4704–4711, 1998). Because some pathogenesis was associated with the vector itself, in the present study we generated new VSV vectors expressing HA which are completely attenuated for pathogenesis in the mouse model. The first vector has a truncation of the cytoplasmic domain of the VSV G protein and expresses influenza virus HA (CT1-HA). This nonpathogenic vector provides complete protection from lethal influenza virus challenge after intranasal administration. A second vector with VSV G deleted and expressing HA (ΔG-HA) is also protective and nonpathogenic and has the advantage of not inducing neutralizing antibodies to the vector itself.
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Mackett, M. "The live vector approach?viruses." World Journal of Microbiology & Biotechnology 7, no. 2 (March 1991): 137–49. http://dx.doi.org/10.1007/bf00328983.
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Ćuk, Marina, Zagorka Savić, Renata Iličić, and Ferenc Bagi. "Importance and epidemiology of tomato spotted wilt virus." Biljni lekar 49, no. 2 (2021): 148–57. http://dx.doi.org/10.5937/biljlek2102148c.
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Tomato spotted wilt virus (TSWV) is the most economically important plant viruses from genus Tospovirus. It has a polyphagous character and infects a wide range of very significant agricultural crops. Vectors of viruses are insects from order Thysanoptera (Thripidae) and till know eight species are known to transmit tospoviruses of which Frankliniella occidentalis is considered to be economically most important vector. TSWV is transmitted by thrips in a persistent and propagative manner. Relationship between vector and TSWV is very specific because vectors acquire the virus in the larval stages, while imago plays a key role in transmission of the virus. TSWV causes wide range of symptoms depending on host plant, external environmental conditions and type of viruses. In addition to affecting the fruit quality of cultivated crops, greatly reduces the yield to agricultural producers. Tomato is the most commonly attacked by TSWV, and after the symptoms manifested on leaves in the form of a bronze color, the virus was name. Protection of agricultural crops is very challenging and difficult due to wide distribution of viruse vectors, their hidden way of life as well as wide range of TSWV hosts.
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Tsai, Chi-Wei, Adib Rowhani, Deborah A. Golino, Kent M. Daane, and Rodrigo P. P. Almeida. "Mealybug Transmission of Grapevine Leafroll Viruses: An Analysis of Virus–Vector Specificity." Phytopathology® 100, no. 8 (August 2010): 830–34. http://dx.doi.org/10.1094/phyto-100-8-0830.
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To understand ecological factors mediating the spread of insect-borne plant pathogens, vector species for these pathogens need to be identified. Grapevine leafroll disease is caused by a complex of phylogenetically related closteroviruses, some of which are transmitted by insect vectors; however, the specificities of these complex virus–vector interactions are poorly understood thus far. Through biological assays and phylogenetic analyses, we studied the role of vector-pathogen specificity in the transmission of several grapevine leafroll-associated viruses (GLRaVs) by their mealybug vectors. Using plants with multiple virus infections, several virus species were screened for vector transmission by the mealybug species Planococcus ficus and Pseudococcus longispinus. We report that two GLRaVs (-4 and -9), for which no vector transmission evidence was available, are mealybug-borne. The analyses performed indicated no evidence of mealybug–GLRaV specificity; for example, different vector species transmitted GLRaV-3 and one vector species, Planococcus ficus, transmitted five GLRaVs. Based on available data, there is no compelling evidence of vector–virus specificity in the mealybug transmission of GLRaVs. However, more studies aimed at increasing the number of mealybug species tested as vectors of different GLRaVs are necessary. This is especially important given the increasing importance of grapevine leafroll disease spread by mealybugs in vineyards worldwide.
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Xu, Beibei, Zhiying Tan, Kenli Li, Taijiao Jiang, and Yousong Peng. "Predicting the host of influenza viruses based on the word vector." PeerJ 5 (July 18, 2017): e3579. http://dx.doi.org/10.7717/peerj.3579.
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Newly emerging influenza viruses continue to threaten public health. A rapid determination of the host range of newly discovered influenza viruses would assist in early assessment of their risk. Here, we attempted to predict the host of influenza viruses using the Support Vector Machine (SVM) classifier based on the word vector, a new representation and feature extraction method for biological sequences. The results show that the length of the word within the word vector, the sequence type (DNA or protein) and the species from which the sequences were derived for generating the word vector all influence the performance of models in predicting the host of influenza viruses. In nearly all cases, the models built on the surface proteins hemagglutinin (HA) and neuraminidase (NA) (or their genes) produced better results than internal influenza proteins (or their genes). The best performance was achieved when the model was built on the HA gene based on word vectors (words of three-letters long) generated from DNA sequences of the influenza virus. This results in accuracies of 99.7% for avian, 96.9% for human and 90.6% for swine influenza viruses. Compared to the method of sequence homology best-hit searches using the Basic Local Alignment Search Tool (BLAST), the word vector-based models still need further improvements in predicting the host of influenza A viruses.
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Khanna, Madhu, Nilanshu Manocha, Garima Joshi, Latika Saxena, and Sanjesh Saini. "Role of retroviral vector-based interventions in combating virus infections." Future Virology 14, no. 7 (July 2019): 473–85. http://dx.doi.org/10.2217/fvl-2018-0151.
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The deployment of viruses as vaccine-vectors has witnessed recent developments owing to a better understanding of viral genomes and mechanism of interaction with the immune system. Vaccine delivery by viral vectors offers various advantages over traditional approaches. Viral vector vaccines are one of the best candidates for activating the cellular arm of the immune system, coupled with the induction of significant humoral responses. Hence, there is a broad scope for the development of effective vaccines against many diseases using viruses as vectors. Further studies are required before an ideal vaccine-vector is developed and licensed for use in humans. In this article, we have outlined the use of retroviral vectors in developing vaccines against various viral diseases.
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Moreno, Aranzazu, Eugénie Hébrard, Marilyne Uzest, Stéphane Blanc, and Alberto Fereres. "A Single Amino Acid Position in the Helper Component of Cauliflower Mosaic Virus Can Change the Spectrum of Transmitting Vector Species." Journal of Virology 79, no. 21 (November 1, 2005): 13587–93. http://dx.doi.org/10.1128/jvi.79.21.13587-13593.2005.
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ABSTRACT Viruses frequently use insect vectors to effect rapid spread through host populations. In plant viruses, vector transmission is the major mode of transmission, used by nearly 80% of species described to date. Despite the importance of this phenomenon in epidemiology, the specificity of the virus-vector relationship is poorly understood at both the molecular and the evolutionary level, and very limited data are available on the precise viral protein motifs that control specificity. Here, using the aphid-transmitted Cauliflower mosaic virus (CaMV) as a biological model, we confirm that the “noncirculative” mode of transmission dominant in plant viruses (designated “mechanical vector transmission” in animal viruses) involves extremely specific virus-vector recognition, and we identify an amino acid position in the “helper component” (HC) protein of CaMV involved in such recognition. Site-directed mutagenesis revealed that changing the residue at this position can differentially affect transmission rates obtained with various aphid species, thus modifying the spectrum of vector species for CaMV. Most interestingly, in a virus line transmitted by a single vector species, we observed the rapid appearance of a spontaneous mutant specifically losing its transmissibility by another aphid species. Hence, in addition to the first identification of an HC motif directly involved in specific vector recognition, we demonstrate that change of a virus to a different vector species requires only a single mutation and can occur rapidly and spontaneously.
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Di Mattia, Jérémy, Faustine Ryckebusch, Marie-Stéphanie Vernerey, Elodie Pirolles, Nicolas Sauvion, Michel Peterschmitt, Jean-Louis Zeddam, and Stéphane Blanc. "Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector." Viruses 12, no. 3 (March 10, 2020): 299. http://dx.doi.org/10.3390/v12030299.
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Single-stranded DNA (ssDNA) plant viruses belong to the families Geminiviridae and Nanoviridae. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner. While geminiviruses are transmitted by leafhoppers, treehoppers, whiteflies and aphids, nanoviruses are transmitted exclusively by aphids. Circulative transmission involves complex virus–vector interactions in which epithelial cells have to be crossed and defense mechanisms counteracted. Vector taxa are considered a relevant taxonomic criterion for virus classification, indicating that viruses can evolve specific interactions with their vectors. Thus, we predicted that, although nanoviruses and geminiviruses represent related viral families, they have evolved distinct interactions with their vector. This prediction is also supported by the non-structural Nuclear Shuttle Protein (NSP) that is involved in vector transmission in nanoviruses but has no similar function in geminiviruses. Thanks to the recent discovery of aphid-transmitted geminiviruses, this prediction could be tested for the geminivirus alfalfa leaf curl virus (ALCV) and the nanovirus faba bean necrotic stunt virus (FBNSV) in their common vector, Aphis craccivora. Estimations of viral load in midgut and head of aphids, precise localization of viral DNA in cells of insect vectors and host plants, and virus transmission tests revealed that the pathway of the two viruses across the body of their common vector differs both quantitatively and qualitatively.
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Chen, Qian, and Taiyun Wei. "Cell Biology During Infection of Plant Viruses in Insect Vectors and Plant Hosts." Molecular Plant-Microbe Interactions® 33, no. 1 (January 2020): 18–25. http://dx.doi.org/10.1094/mpmi-07-19-0184-cr.
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Plant viruses typically cause severe pathogenicity in plants, even resulting in the death of plants. Many pathogenic plant viruses are transmitted in a persistent manner via insect vectors. Interestingly, unlike in the plant hosts, persistent viruses are either nonpathogenic or show limited pathogenicity in their insect vectors, while taking advantage of the cellular machinery of insect vectors for completing their life cycles. This review discusses why persistent plant viruses are nonpathogenic or have limited pathogenicity to their insect vectors while being pathogenic to plants hosts. Current advances in cell biology of virus–insect vector interactions are summarized, including virus-induced inclusion bodies, changes of insect cellular ultrastructure, and immune response of insects to the viruses, especially autophagy and apoptosis. The corresponding findings of virus-plant interactions are compared. An integrated view of the balance strategy achieved by the interaction between viral attack and the immune response of insect is presented. Finally, we outline progress gaps between virus-insect and virus-plant interactions, thus highlighting the contributions of cultured cells to the cell biology of virus-insect interactions. Furthermore, future prospects of studying the cell biology of virus-vector interactions are presented.
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Chung, Bong-Nam, Tomas Canto, and Peter Palukaitis. "Stability of recombinant plant viruses containing genes of unrelated plant viruses." Journal of General Virology 88, no. 4 (April 1, 2007): 1347–55. http://dx.doi.org/10.1099/vir.0.82477-0.
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The stability of hybrid plant viruses that might arise by recombination in transgenic plants was examined using hybrid viruses derived from the viral expression vectors potato virus X (PVX) and tobacco rattle virus (TRV). The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was introduced into a vector derived from TRV RNA2 to generate TRV-NIb. All three viruses were unstable and most of the progeny viruses had lost the inserted sequences between 2 and 4 weeks post-inoculation. There was some variation in the rate of loss of part or all of the inserted sequence and the number of plants containing the deleted viruses, depending on the sequence, the host (Nicotiana tabacum vs Nicotiana benthamiana) or the vector, although none of these factors was associated consistently with the preferential loss of the inserted sequences. PVX-NIb was unable to accumulate in NIb-transgenic tobacco resistant to infection by PVY and also showed loss of the NIb insert from PVX-NIb in some NIb-transgenic tobacco plants susceptible to infection by PVY. These data indicate that such hybrid viruses, formed in resistant transgenic plants from a transgene and an unrelated virus, would be at a selective disadvantage, first by being targeted by the resistance mechanism and second by not being competitive with the parental virus.
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Mackenzie, John S., and Andrew F. van den Hurk. "The risks to Australia from emerging and exotic arboviruses." Microbiology Australia 39, no. 2 (2018): 84. http://dx.doi.org/10.1071/ma18023.
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The recent pandemic spread of mosquito-borne arboviruses across multiple continents, as exemplified by West Nile (WNV)1,, chikungunya (CHIKV)2, and Zika (ZIKV)3, viruses, together with the continuing disease burden of epidemic dengue viruses (DENVs)1, multiple importations of yellow fever virus (YFV) into populous areas of Asia4, and the potential threat of some other, possibly unknown, emerging arboviral threat, constitute a wake-up call for governments to strengthen surveillance programmes and enhance research into mosquito-transmitted diseases5–7. Rift Valley fever8 (RVFV) and Japanese encephalitis1,9 (JEV) viruses are also important examples of threats to human and/or livestock health. Australia is vulnerable to these arboviral diseases, with risk of importation and outbreak potential varying between viruses10. The risk of exotic arboviral diseases establishing transmission cycles in Australia is dependent on the availability of competent vectors and suitable vertebrate hosts. Therefore, knowledge of the vector competence of Australian mosquito species for exotic arboviruses, potential for the introduction and establishment of exotic vector species, and suitability of vertebrate hosts, are essential components of understanding and mitigating these arboviral threats.
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Zhao, Pingzhi, Xiangmei Yao, Congxi Cai, Ran Li, Jie Du, Yanwei Sun, Mengyu Wang, et al. "Viruses mobilize plant immunity to deter nonvector insect herbivores." Science Advances 5, no. 8 (August 2019): eaav9801. http://dx.doi.org/10.1126/sciadv.aav9801.
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A parasite-infected host may promote performance of associated insect vectors; but possible parasite effects on nonvector insects have been largely unexplored. Here, we show that Begomovirus, the largest genus of plant viruses and transmitted exclusively by whitefly, reprogram plant immunity to promote the fitness of the vector and suppress performance of nonvector insects (i.e., cotton bollworm and aphid). Infected plants accumulated begomoviral βC1 proteins in the phloem where they were bound to the plant transcription factor WRKY20. This viral hijacking of WRKY20 spatiotemporally redeployed plant chemical immunity within the leaf and had the asymmetrical benefiting effects on the begomoviruses and its whitefly vectors while negatively affecting two nonvector competitors. This type of interaction between a parasite and two types of herbivores, i.e., vectors and nonvectors, occurs widely in various natural and agricultural ecosystems; thus, our results have broad implications for the ecological significance of parasite-vector-host tripartite interactions.
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German, Thomas L., Marcé D. Lorenzen, Nathaniel Grubbs, and Anna E. Whitfield. "New Technologies for Studying Negative-Strand RNA Viruses in Plant and Arthropod Hosts." Molecular Plant-Microbe Interactions® 33, no. 3 (March 2020): 382–93. http://dx.doi.org/10.1094/mpmi-10-19-0281-fi.
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The plant viruses in the phylum Negarnaviricota, orders Bunyavirales and Mononegavirales, have common features of single-stranded, negative-sense RNA genomes and replication in the biological vector. Due to the similarities in biology, comparative functional analysis in plant and vector hosts is helpful for understanding host–virus interactions for negative-strand RNA viruses. In this review, we will highlight recent technological advances that are breaking new ground in the study of these recalcitrant virus systems. The development of infectious clones for plant rhabdoviruses and bunyaviruses is enabling unprecedented examination of gene function in plants and these advances are also being transferred to study virus biology in the vector. In addition, genome and transcriptome projects for critical nonmodel arthropods has enabled characterization of insect response to viruses and identification of interacting proteins. Functional analysis of genes using genome editing will provide future pathways for further study of the transmission cycle and new control strategies for these viruses and their vectors.
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Ziegler-Graff, Véronique. "Molecular Insights into Host and Vector Manipulation by Plant Viruses." Viruses 12, no. 3 (February 27, 2020): 263. http://dx.doi.org/10.3390/v12030263.
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Plant viruses rely on both host plant and vectors for a successful infection. Essentially to simplify studies, transmission has been considered for decades as an interaction between two partners, virus and vector. This interaction has gained a third partner, the host plant, to establish a tripartite pathosystem in which the players can react with each other directly or indirectly through changes induced in/by the third partner. For instance, viruses can alter the plant metabolism or plant immune defence pathways to modify vector’s attraction, settling or feeding, in a way that can be conducive for virus propagation. Such changes in the plant physiology can also become favourable to the vector, establishing a mutualistic relationship. This review focuses on the recent molecular data on the interplay between viral and plant factors that provide some important clues to understand how viruses manipulate both the host plants and vectors in order to improve transmission conditions and thus ensuring their survival.
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Barsov, Eugene V., William S. Payne, and Stephen H. Hughes. "Adaptation of Chimeric Retroviruses In Vitro and In Vivo: Isolation of Avian Retroviral Vectors with Extended Host Range." Journal of Virology 75, no. 11 (June 1, 2001): 4973–83. http://dx.doi.org/10.1128/jvi.75.11.4973-4983.2001.
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ABSTRACT We have designed and characterized two new replication-competent avian sarcoma/leukosis virus-based retroviral vectors with amphotropic and ecotropic host ranges. The amphotropic vector RCASBP-M2C(797-8), was obtained by passaging the chimeric retroviral vector RCASBP-M2C(4070A) (6) in chicken embryos. The ecotropic vector, RCASBP(Eco), was created by replacing theenv-coding region in the retroviral vector RCASBP(A) with the env region from an ecotropic murine leukemia virus. It replicates efficiently in avian DFJ8 cells that express murine ecotropic receptor. For both vectors, permanent cell lines that produce viral stocks with titers of about 5 × 106 CFU/ml on mammalian cells can be easily established by passaging transfected avian cells. Some chimeric viruses, for example, RCASBP(Eco), replicate efficiently without modifications. For those chimeric viruses that do require modification, adaptation by passage in vitro or in vivo is a general strategy. This strategy has been used to prepare vectors with altered host range and could potentially be used to develop vectors that would be useful for targeted gene delivery.
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Fiallo-Olivé, Elvira, Li-Long Pan, Shu-Sheng Liu, and Jesús Navas-Castillo. "Transmission of Begomoviruses and Other Whitefly-Borne Viruses: Dependence on the Vector Species." Phytopathology® 110, no. 1 (January 2020): 10–17. http://dx.doi.org/10.1094/phyto-07-19-0273-fi.
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Most plant viruses require a biological vector to spread from plant to plant in nature. Among biological vectors for plant viruses, hemipteroid insects are the most common, including phloem-feeding aphids, whiteflies, mealybugs, planthoppers, and leafhoppers. A majority of the emerging diseases challenging agriculture worldwide are insect borne, with those transmitted by whiteflies (Hemiptera: Aleyrodidae) topping the list. Most damaging whitefly-transmitted viruses include begomoviruses (Geminiviridae), criniviruses (Closteroviridae), and torradoviruses (Secoviridae). Among the whitefly vectors, Bemisia tabaci, now recognized as a complex of cryptic species, is the most harmful in terms of virus transmission. Here, we review the available information on the differential transmission efficiency of begomoviruses and other whitefly-borne viruses by different species of whiteflies, including the cryptic species of the B. tabaci complex. In addition, we summarize the factors affecting transmission of viruses by whiteflies and point out some future research prospects.
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Hromic-Jahjefendic, Altijana, and Kenneth Lundstrom. "Viral Vector-Based Melanoma Gene Therapy." Biomedicines 8, no. 3 (March 16, 2020): 60. http://dx.doi.org/10.3390/biomedicines8030060.
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Gene therapy applications of oncolytic viruses represent an attractive alternative for cancer treatment. A broad range of oncolytic viruses, including adenoviruses, adeno-associated viruses, alphaviruses, herpes simplex viruses, retroviruses, lentiviruses, rhabdoviruses, reoviruses, measles virus, Newcastle disease virus, picornaviruses and poxviruses, have been used in diverse preclinical and clinical studies for the treatment of various diseases, including colon, head-and-neck, prostate and breast cancer as well as squamous cell carcinoma and glioma. The majority of studies have focused on immunotherapy and several drugs based on viral vectors have been approved. However, gene therapy for malignant melanoma based on viral vectors has not been utilized to its full potential yet. This review represents a summary of the achievements of preclinical and clinical studies using viral vectors, with the focus on malignant melanoma.
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Gray, Stewart M., Dawn M. Smith, Lia Barbierri, and John Burd. "Virus Transmission Phenotype Is Correlated with Host Adaptation Among Genetically Diverse Populations of the Aphid Schizaphis graminum." Phytopathology® 92, no. 9 (September 2002): 970–75. http://dx.doi.org/10.1094/phyto.2002.92.9.970.
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Schizaphis graminum is an important insect pest of several grain crops and an efficient vector of cereal-infecting luteoviruses and poleroviruses. We examined the virus transmission characteristics of several distinct populations and various developmental stages of the aphid. Seven well-characterized S. graminum biotypes maintained at the USDA-ARS laboratory in Stillwater, OK, and two biotypes maintained in New York (one collected in Wisconsin and the other collected in South Carolina) were tested for their ability to transmit five viruses that cause barley yellow dwarf disease (BYD). Four of the Oklahoma biotypes, which do not commonly colonize agronomic crops, and the Wisconsin biotype, were efficient vectors of several viruses. The three other Oklahoma biotypes, which do colonize agronomic crops, and the South Carolina biotype, were poor vectors of all five viruses. Thus, the vector specificity long associated with viruses causing BYD is not limited to the level of aphid species; it clearly extends to populations within a single species. S. graminum nymphs are reported to be more efficient vectors of Barley yellow dwarf virus (BYDV-SGV) than are adults. This was confirmed only for the Wisconsin biotype, but not for the other eight S. graminum biotypes. Thus, there does not appear to be a generalized developmentally regulated barrier to the transmission of BYDV-SGV in S. graminum. Furthermore, the developmentally regulated vector competency observed in the Wisconsin biotype did not extend to other viruses. BYDV-PAV and Cereal yellow dwarf virus-RPV were transmitted with similar efficiency by all S. graminum biotypes when acquired by nymphs or adults.
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Whitfield, Anna E., Bryce W. Falk, and Dorith Rotenberg. "Insect vector-mediated transmission of plant viruses." Virology 479-480 (May 2015): 278–89. http://dx.doi.org/10.1016/j.virol.2015.03.026.
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Hogenhout, Saskia A., El-Desouky Ammar, Anna E. Whitfield, and Margaret G. Redinbaugh. "Insect Vector Interactions with Persistently Transmitted Viruses." Annual Review of Phytopathology 46, no. 1 (September 2008): 327–59. http://dx.doi.org/10.1146/annurev.phyto.022508.092135.
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Pirone, Thomas P., and Stéphane Blanc. "HELPER-DEPENDENT VECTOR TRANSMISSION OF PLANT VIRUSES." Annual Review of Phytopathology 34, no. 1 (September 1996): 227–47. http://dx.doi.org/10.1146/annurev.phyto.34.1.227.
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Reis, Nelson Nogueira, Alcino Lázaro da Silva, Elma Pereira Guedes Reis, Flávia Chaves e. Silva, and Igor Guedes Nogueira Reis. "Viruses vector control proposal: genus Aedes emphasis." Brazilian Journal of Infectious Diseases 21, no. 4 (July 2017): 457–63. http://dx.doi.org/10.1016/j.bjid.2017.03.020.
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Miyoshi, Hiroyuki, Ulrike Blömer, Masayo Takahashi, Fred H. Gage, and Inder M. Verma. "Development of a Self-Inactivating Lentivirus Vector." Journal of Virology 72, no. 10 (October 1, 1998): 8150–57. http://dx.doi.org/10.1128/jvi.72.10.8150-8157.1998.
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ABSTRACT We have constructed a new series of lentivirus vectors based on human immunodeficiency virus type 1 (HIV-1) that can transduce nondividing cells. The U3 region of the 5′ long terminal repeat (LTR) in vector constructs was replaced with the cytomegalovirus (CMV) promoter, resulting in Tat-independent transcription but still maintaining high levels of expression. A self-inactivating (SIN) vector was constructed by deleting 133 bp in the U3 region of the 3′ LTR, including the TATA box and binding sites for transcription factors Sp1 and NF-κB. The deletion is transferred to the 5′ LTR after reverse transcription and integration in infected cells, resulting in the transcriptional inactivation of the LTR in the proviruses. SIN viruses can be generated with no significant decreases in titer. Injection of viruses into the rat brain showed that a SIN vector containing the green fluorescent protein gene under the control of the internal CMV promoter transduced neurons as efficiently as a wild-type vector. Interestingly, a wild-type vector without an internal promoter also successfully transduced neurons in the brain, indicating that the HIV-1 LTR promoter is transcriptionally active in neurons even in the absence of Tat. Furthermore, injection of viruses into the subretinal space of the rat eye showed that wild-type vector transduced predominantly retinal pigment epithelium and photoreceptor cells, while SIN vector was able to transduce other types of retinal cells, including bipolar, Müller, horizontal, and amacrine cells. This finding suggests that the HIV-1 LTR can negatively influence the internal CMV promoter in some cell types. SIN HIV vectors should be safer for gene therapy, and they also have broader applicability as a means of high-level gene transfer and expression in nondividing cells.
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Lan, Hanhong, Hongyan Chen, Yuyan Liu, Chaoyang Jiang, Qianzhuo Mao, Dongsheng Jia, Qian Chen, and Taiyun Wei. "Small Interfering RNA Pathway Modulates Initial Viral Infection in Midgut Epithelium of Insect after Ingestion of Virus." Journal of Virology 90, no. 2 (November 4, 2015): 917–29. http://dx.doi.org/10.1128/jvi.01835-15.
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ABSTRACTNumerous viruses are transmitted in a persistent manner by insect vectors. Persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. Although propagation of viruses in insect vectors can be controlled by the small interfering RNA (siRNA) antiviral pathway, whether the siRNA pathway can control viral dissemination from the midgut epithelium is unknown. Infection by a rice virus (Southern rice black streaked dwarf virus[SRBSDV]) of its incompetent vector (the small brown planthopper [SBPH]) is restricted to the midgut epithelium. Here, we show that the siRNA pathway is triggered by SRBSDV infection in continuously cultured cells derived from the SBPH and in the midgut of the intact insect. Knockdown of the expression of the core component Dicer-2 of the siRNA pathway by RNA interference strongly increased the ability of SRBSDV to propagate in continuously cultured SBPH cells and in the midgut epithelium, allowing viral titers in the midgut epithelium to reach the threshold (1.99 × 109copies of the SRBSDVP10gene/μg of midgut RNA) needed for viral dissemination into the SBPH midgut muscles. Our results thus represent the first elucidation of the threshold for viral dissemination from the insect midgut epithelium. Silencing of Dicer-2 further facilitated the transmission of SRBSDV into rice plants by SBPHs. Taken together, our results reveal the new finding that the siRNA pathway can control the initial infection of the insect midgut epithelium by a virus, which finally affects the competence of the virus's vector.IMPORTANCEMany viral pathogens that cause significant global health and agricultural problems are transmitted via insect vectors. The first bottleneck in viral infection, the midgut epithelium, is a principal determinant of the ability of an insect species to transmit a virus.Southern rice black streaked dwarf virus(SRBSDV) is restricted exclusively to the midgut epithelium of an incompetent vector, the small brown planthopper (SBPH). Here, we show that silencing of the core component Dicer-2 of the small interfering RNA (siRNA) pathway increases viral titers in the midgut epithelium past the threshold (1.99 × 109copies of the SRBSDVP10gene/μg of midgut RNA) for viral dissemination into the midgut muscles and then into the salivary glands, allowing the SBPH to become a competent vector of SRBSDV. This result is the first evidence that the siRNA antiviral pathway has a direct role in the control of viral dissemination from the midgut epithelium and that it affects the competence of the virus's vector.
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Van den Broeke, Anne, and Arsène Burny. "Retroviral Vector Biosafety: Lessons from Sheep." Journal of Biomedicine and Biotechnology 2003, no. 1 (2003): 9–12. http://dx.doi.org/10.1155/s1110724303209128.
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The safety of retroviral-based systems and the possible transmission of replication-competent virus to patients is a major concern associated with using retroviral vectors for gene therapy. While much effort has been put into the design of safe retroviral production methods and effective in vitro monitoring assays, there is little data evaluating the risks resulting from retroviral vector instability at post-transduction stages especially following in vivo gene delivery. Here, we briefly describe and discuss our observations in an in vivo experimental model based on the inoculation of retroviral vector-transduced tumor cells in sheep. Our data indicates that the in vivo generation of mosaic viruses is a dynamic process and that virus variants, generated by retroviral vector-mediated recombination, may be stored and persist in infected individuals prior to selection at the level of replication. Recombination may not only restore essential viral functions or provide selective advantages in a changing environment but also reestablish or enhance the pathogenic potential of the particular virus undergoing recombination. These observations in sheep break new ground in our understanding of how retroviral vectors may have an impact on the course of a preestablished disease or reactivate dormant or endogenous viruses. The in vivo aspects of vector stability raise important biosafety issues for the future development of safe retroviral vector-based gene therapy.
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He, Ya-Zhou, Yu-Meng Wang, Tian-Yan Yin, Elvira Fiallo-Olivé, Yin-Quan Liu, Linda Hanley-Bowdoin, and Xiao-Wei Wang. "A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery." Proceedings of the National Academy of Sciences 117, no. 29 (July 7, 2020): 16928–37. http://dx.doi.org/10.1073/pnas.1820132117.
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Whereas most of the arthropod-borne animal viruses replicate in their vectors, this is less common for plant viruses. So far, only some plant RNA viruses have been demonstrated to replicate in insect vectors and plant hosts. How plant viruses evolved to replicate in the animal kingdom remains largely unknown. Geminiviruses comprise a large family of plant-infecting, single-stranded DNA viruses that cause serious crop losses worldwide. Here, we report evidence and insight into the replication of the geminivirus tomato yellow leaf curl virus (TYLCV) in the whitefly (Bemisia tabaci) vector and that replication is mainly in the salivary glands. We found that TYLCV induces DNA synthesis machinery, proliferating cell nuclear antigen (PCNA) and DNA polymerase δ (Polδ), to establish a replication-competent environment in whiteflies. TYLCV replication-associated protein (Rep) interacts with whitefly PCNA, which recruits DNA Polδ for virus replication. In contrast, another geminivirus, papaya leaf curl China virus (PaLCuCNV), does not replicate in the whitefly vector. PaLCuCNV does not induce DNA-synthesis machinery, and the Rep does not interact with whitefly PCNA. Our findings reveal important mechanisms by which a plant DNA virus replicates across the kingdom barrier in an insect and may help to explain the global spread of this devastating pathogen.
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Jeon, Yoondeok, Jiwoo Oh, Seungjae Lim, Yewon Choi, Sungmoon Kim, and Taeseon Yoon. "Analysis of Structural Relationship between Immunodeficiency Viruses Using Support Vector Machine." International Journal of Computer Theory and Engineering 7, no. 1 (February 2014): 46–50. http://dx.doi.org/10.7763/ijcte.2015.v7.928.
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Tapio, Eeva. "The appearance of soil-borne viruses in Finnish plant nurseries II." Agricultural and Food Science 57, no. 3 (September 1, 1985): 167–81. http://dx.doi.org/10.23986/afsci.72199.
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In the beginning of the 1970’s, the occurrence of soil-borne viruses in 30 Finnish nurseries and experimental fields of garden plants at 3 research stations was mapped. Viruses were isolated on 26.9 % of the 672 plant and soil samples collected. The two most commonly found viruses were tobacco necrosis virus (TNV), 42.5 %, and tobacco rattle virus (TRV), 23.7 %. Tomato black ring virus (TBRV) and raspberry ringspot virus (RRSV) were isolated for the first time in Finland. The abundant occurence of TBRV in 32 samples was due to the abundance of Phlox paniculata samples. RRSV was isolated from only a few samples. The vectors of all of the above-mentioned viruses were found in many samples. The fungus vector of TNV, Olpidium brassicae, was investigated by examining the roots microscopically. The vector of TRV, the Trichodorus sp. nematodes, and the vector of TBRV and RRSV, the Longidorus sp. nematodes, were isolated from soil samples. In addition to the foregoing, tobacco mosaic virus was isolated from 31 samples of 6 nurseries and 2 experimental fields. Viruses were isolated from many weed samples, especially from roots of Senecio vulgaris and Stellaria media. Perennials proved to be virotic. All of the above mentioned viruses, especially TBRV and TRV, were isolated from Phlox paniculata; TBRV was also found in an Astilbe x arendsii sample. Dicentra spectabilis, like Phlox, was commonly infected with TRV. No clear results could be obtained from control experiments.
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Allen, Linda J. S., Vrushali A. Bokil, Nik J. Cunniffe, Frédéric M. Hamelin, Frank M. Hilker, and Michael J. Jeger. "Modelling Vector Transmission and Epidemiology of Co-Infecting Plant Viruses." Viruses 11, no. 12 (December 13, 2019): 1153. http://dx.doi.org/10.3390/v11121153.
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Co-infection of plant hosts by two or more viruses is common in agricultural crops and natural plant communities. A variety of models have been used to investigate the dynamics of co-infection which track only the disease status of infected and co-infected plants, and which do not explicitly track the density of inoculative vectors. Much less attention has been paid to the role of vector transmission in co-infection, that is, acquisition and inoculation and their synergistic and antagonistic interactions. In this investigation, a general epidemiological model is formulated for one vector species and one plant species with potential co-infection in the host plant by two viruses. The basic reproduction number provides conditions for successful invasion of a single virus. We derive a new invasion threshold which provides conditions for successful invasion of a second virus. These two thresholds highlight some key epidemiological parameters important in vector transmission. To illustrate the flexibility of our model, we examine numerically two special cases of viral invasion. In the first case, one virus species depends on an autonomous virus for its successful transmission and in the second case, both viruses are unable to invade alone but can co-infect the host plant when prevalence is high.
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Johnson, Teresa R., Julie E. Fischer, and Barney S. Graham. "Construction and characterization of recombinant vaccinia viruses co-expressing a respiratory syncytial virus protein and a cytokine." Journal of General Virology 82, no. 9 (September 1, 2001): 2107–16. http://dx.doi.org/10.1099/0022-1317-82-9-2107.
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Recombinant vaccinia viruses are well-characterized tools that can be used to define novel approaches to vaccine formulation and delivery. While vector co-expression of immune mediators has enormous potential for optimizing the composition of vaccine-induced immune responses, the impact on antigen expression and vector antigenicity must also be considered. Co-expression of IL-4 increased vaccinia virus vector titres, while IFN-γ co-expression reduced vaccinia virus replication in BALB/c mice and in C57BL/6 mice infected with some recombinant viruses. Protection against respiratory syncytial virus (RSV) challenge was similar in mice immunized with vaccinia virus expressing RSV G glycoprotein and IFN-γ, even though the replication efficiency of the vector was diminished. These data demonstrate the ability of vector-expressed cytokine to influence the virulence of the vector and to direct the development of selected immune responses. This suggests that the co-expression of cytokines and other immunomodulators has the potential to improve the safety of vaccine vectors while improving the immunogenicity of vaccine antigens.
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Gallet, Romain, Yannis Michalakis, and Stéphane Blanc. "Vector-transmission of plant viruses and constraints imposed by virus–vector interactions." Current Opinion in Virology 33 (December 2018): 144–50. http://dx.doi.org/10.1016/j.coviro.2018.08.005.
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Schoggins, John W., Jason G. D. Gall, and Erik Falck-Pedersen. "Subgroup B and F Fiber Chimeras Eliminate Normal Adenovirus Type 5 Vector Transduction In Vitro and In Vivo." Journal of Virology 77, no. 2 (January 15, 2003): 1039–48. http://dx.doi.org/10.1128/jvi.77.2.1039-1048.2003.
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ABSTRACT Altering adenovirus vector (Ad vector) targeting is an important goal for a variety of gene therapy applications and involves eliminating or reducing the normal tropism of a vector and retargeting through a distinct receptor-ligand pathway. The first step of Ad vector infection is high-affinity binding to a target cellular receptor. For the majority of adenoviruses and Ad vectors, the fiber capsid protein serves this purpose, binding to the coxsackievirus and adenovirus receptor (CAR) present on a variety of cell types. In this study we have explored a novel approach to altering Ad type 5 (Ad5) vector targeting based on serotypic differences in fiber function. The subgroup B viruses bind to an unidentified receptor that is distinct from CAR. The subgroup F viruses are the only adenoviruses that express two distinct terminal exons encoding fiber open reading frames. We have constructed chimeric fiber adenoviruses that utilize the tandem fiber arrangement of the subgroup F genome configuration. By taking advantage of serotypic differences in fiber expression, fiber shaft length, and fiber binding efficiency, we have developed a tandem fiber vector that has low binding efficiency for the known fiber binding sites, does not rely on an Ad5-based fiber, and can be grown to high titer using conventional cell lines. Importantly, when characterizing these vectors in vivo, we find the subgroup B system and our optimal tandem fiber system demonstrate reduced liver transduction by over 2 logs compared to an Ad5 fiber vector. These attributes make the tandem fiber vector a useful alternative to conventional strategies for fiber manipulation of adenovirus vectors.
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Mittapelly, Priyanka, and Swapna Priya Rajarapu. "Applications of Proteomic Tools to Study Insect Vector–Plant Virus Interactions." Life 10, no. 8 (August 7, 2020): 143. http://dx.doi.org/10.3390/life10080143.
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Proteins are crucial players of biological interactions within and between the organisms and thus it is important to understand the role of proteins in successful partnerships, such as insect vectors and their plant viruses. Proteomic approaches have identified several proteins at the interface of virus acquisition and transmission by their insect vectors which could be potential molecular targets for sustainable pest and viral disease management strategies. Here we review the proteomic techniques used to study the interactions of insect vector and plant virus. Our review will focus on the techniques available to identify the infection, global changes at the proteome level in insect vectors, and protein-protein interactions of insect vectors and plant viruses. Furthermore, we also review the integration of other techniques with proteomics and the available bioinformatic tools to analyze the proteomic data.
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Hall, Roy A., and Jody Hobson-Peters. "Newly discovered mosquito viruses help control vector-borne viral diseases." Microbiology Australia 39, no. 2 (2018): 72. http://dx.doi.org/10.1071/ma18020.
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Many well-known mosquito-borne viruses such as dengue, Zika, West Nile, chikungunya and Ross River viruses can be transmitted to vertebrates and are associated with disease in man or animals. However, the use of deep sequencing and other open-minded approaches to detect viruses in mosquitoes have uncovered many new RNA viruses, most of which do not infect vertebrates. The discovery of these ‘insect-specific' viruses (ISVs) has redefined the mosquito virome and prompted the lines of viral taxonomic classification to be redrawn1,2. Despite their benign phenotype, ISVs have become a hot topic of research, with recent studies indicating they have significant application for biotechnology.
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Nuttall, P. A. "Displaced tick-parasite interactions at the host interface." Parasitology 116, S1 (1998): S65—S72. http://dx.doi.org/10.1017/s003118200008495x.
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SummaryReciprocal interactions of parasites transmitted by blood-sucking arthropod vectors have been studied primarily at the parasite–host and parasite–vector interface. The third component of this parasite triangle, the vector–host interface, has been largely ignored. Now there is growing realization that reciprocal interactions between arthropod vectors and their vertebrate hosts play a pivotal role in the survival of arthropod-borne viruses, bacteria, and protozoa. The vector–host interface is the site where the haematophagous arthropod feeds. To obtain a blood meal, the vector must overcome the host's inflammatory, haemostatic, and immune responses. This problem is greatest for ixodid ticks which may imbibe as much as 15 ml blood whilst continuously attached to their host for 10 days or more. To feed successfully, the interface between tick and host becomes a battle between the host's mechanisms for combating the tick and the tick's armoury of bioactive proteins and other chemicals which it secretes, via saliva, into the feeding lesion formed in the host's skin. Parasites entering this battlefield encounter a privileged site in their vertebrate host that has been profoundly modified by the pharmacological activities of their vector's saliva. For example, ticks suppress natural killer cells and interferons, both of which have potent antiviral activities. Not surprisingly, vector-borne parasites exploit the immunomodulated feeding site to promote their transmission and infection. Certain tick-borne viruses are so successful at this that they are transmitted from one infected tick, through the vertebrate host to a co-feeding uninfected tick, without a detectable viraemia (virus circulating in the host's blood), and with no untoward effect on the host. When such viruses do have an adverse effect on the host, they may impede their vectors' feeding. Thus important interactions between ticks and tick-borne parasites are displaced to the interface with their vertebrate host - the skin site of blood-feeding and infection.
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Hampton, Richard. "COWPEA VIRUSES, INDIGENOUS AND EXOTIC, AND UNIQUE MECHANISMS BY WHICH THEY ARE DISSEMINATED AND INADVERTENTLY INTRODUCED." HortScience 26, no. 5 (May 1991): 493g—493. http://dx.doi.org/10.21273/hortsci.26.5.493g.
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Vectors with specific vector-virus relationships (e.g., aphid, beetle, thrip, nematode) commonly cause short-range dissemination of cowpea viruses. However, viruses that are seed-borne in cowpea can be disseminated around the world in a single year through seed shipments. Likewise, increased world emphasis on germplasm collection and exchange, for development of improved crop cultivars, increases the risk of disseminating seed-borne viruses in germplasm. Seed-borne cowpea viruses that are not reported in the U.S.A., but are apt to occur in Vigna unguiculata from world centers of cowpea origin include COWPEA APHID-BORNE MOSAIC, COWPEA MILD MOTTLE, COWPEA MOSAIC, and COWPEA MOTTLE VIRUSES. All of these viruses were detected by ELISA serology in V. unguiculata seedlots processed as potential germplasm introductions, in collaboration with the government of Denmark. Germplasm-borne viruses, once introduced into breeding programs, may be seed-transmitted directly into breeding progenies, along with genes derived from the germplasm source. Such viruses also may be spread by insect vectors to other breeding lines, and could cause disease outbreaks to nearby commercial cowpea crops.
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Agranovsky, Alexey. "Enhancing Capsid Proteins Capacity in Plant Virus-Vector Interactions and Virus Transmission." Cells 10, no. 1 (January 7, 2021): 90. http://dx.doi.org/10.3390/cells10010090.
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Vector transmission of plant viruses is basically of two types that depend on the virus helper component proteins or the capsid proteins. A number of plant viruses belonging to disparate groups have developed unusual capsid proteins providing for interactions with the vector. Thus, cauliflower mosaic virus, a plant pararetrovirus, employs a virion associated p3 protein, the major capsid protein, and a helper component for the semi-persistent transmission by aphids. Benyviruses encode a capsid protein readthrough domain (CP-RTD) located at one end of the rod-like helical particle, which serves for the virus transmission by soil fungal zoospores. Likewise, the CP-RTD, being a minor component of the luteovirus icosahedral virions, provides for persistent, circulative aphid transmission. Closteroviruses encode several CPs and virion-associated proteins that form the filamentous helical particles and mediate transmission by aphid, whitefly, or mealybug vectors. The variable strategies of transmission and evolutionary ‘inventions’ of the unusual capsid proteins of plant RNA viruses are discussed.
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Agranovsky, Alexey. "Enhancing Capsid Proteins Capacity in Plant Virus-Vector Interactions and Virus Transmission." Cells 10, no. 1 (January 7, 2021): 90. http://dx.doi.org/10.3390/cells10010090.
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Vector transmission of plant viruses is basically of two types that depend on the virus helper component proteins or the capsid proteins. A number of plant viruses belonging to disparate groups have developed unusual capsid proteins providing for interactions with the vector. Thus, cauliflower mosaic virus, a plant pararetrovirus, employs a virion associated p3 protein, the major capsid protein, and a helper component for the semi-persistent transmission by aphids. Benyviruses encode a capsid protein readthrough domain (CP-RTD) located at one end of the rod-like helical particle, which serves for the virus transmission by soil fungal zoospores. Likewise, the CP-RTD, being a minor component of the luteovirus icosahedral virions, provides for persistent, circulative aphid transmission. Closteroviruses encode several CPs and virion-associated proteins that form the filamentous helical particles and mediate transmission by aphid, whitefly, or mealybug vectors. The variable strategies of transmission and evolutionary ‘inventions’ of the unusual capsid proteins of plant RNA viruses are discussed.
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Mutisya, James, Michael Kahato, Francis Mulwa, Solomon Langat, Edith Chepkorir, Samuel Arum, David Tchouassi, Rosemary Sang, and Joel Lutomiah. "Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses." PLOS ONE 16, no. 7 (July 1, 2021): e0253955. http://dx.doi.org/10.1371/journal.pone.0253955.
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Background Bunyamwera(BUNV) and Ngari (NGIV) viruses are arboviruses of medical importance globally, the viruses are endemic in Africa, Aedes(Ae) aegypti and Anopheles(An) gambiae mosquitoes are currently competent vectors for BUNV and NGIV respectively. Both viruses have been isolated from humans and mosquitoes in various ecologies of Kenya. Understanding the risk patterns and spread of the viruses necessitate studies of vector competence in local vector population of Ae. simpsoni sl which is abundant in the coastal region. This study sought to assess the ability of Ae. Simpsoni sl mosquitoes abundant at the Coast of Kenya to transmit these viruses in experimental laboratory experiments. Methods Field collected larvae/pupae of Ae. Simpsoni sl mosquitoes from Rabai, Kilifi County, were reared to adults, the first filial generation (F0) females’ mosquitoes were orally exposed to infectious blood meal with isolates of the viruses using the hemotek membrane feeder. The exposed mosquitoes were incubated under insectary conditions and sampled on day 7, 14 and 21days post infection to determine susceptibility to the virus infection using plaque assay. Results A total of 379 (Bunyamwera virus 255 and Ngari virus 124) Ae. simpsoni sl were orally exposed to infectious blood meal. Overall, the infection rate (IR) for BUNV and NGIV were 2.7 and 0.9% respectively. Dissemination occurred in 5 out 7 mosquitoes with mid-gut infection for Bunyamwera virus and 1 out of 2 mosquitoes with mid-gut infection for Ngari virus. Further, the transmission was observed in 1 out of 5 mosquitoes that had disseminated infection and no transmission was observed for Ngari virus in all days post infection (dpi). Conclusion Our study shows that Ae. simpsoni sl. is a laboratory competent vector for Bunyamwera virus since it was able to transmit the virus through capillary feeding while NGIV infection was restricted to midgut infection and disseminated infection, these finding adds information on the epidemiology of the viruses and vector control plan.
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Minh, Aline, and Amine A. Kamen. "Critical Assessment of Purification and Analytical Technologies for Enveloped Viral Vector and Vaccine Processing and Their Current Limitations in Resolving Co-Expressed Extracellular Vesicles." Vaccines 9, no. 8 (July 26, 2021): 823. http://dx.doi.org/10.3390/vaccines9080823.
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Viral vectors and viral vaccines are invaluable tools in prevention and treatment of diseases. Many infectious diseases are controlled using vaccines designed from subunits or whole viral structures, whereas other genetic diseases and cancers are being treated by viruses used as vehicles for delivering genetic material in gene therapy or as therapeutic agents in virotherapy protocols. Viral vectors and vaccines are produced in different platforms, from traditional embryonated chicken eggs to more advanced cell cultures. All these expression systems, like most cells and cellular tissues, are known to spontaneously release extracellular vesicles (EVs). EVs share similar sizes, biophysical characteristics and even biogenesis pathways with enveloped viruses, which are currently used as key ingredients in a number of viral vectors and licensed vaccine products. Herein, we review distinctive features and similarities between EVs and enveloped viruses as we revisit the downstream processing steps and analytical technologies currently implemented to produce and document viral vector and vaccine products. Within a context of well-established viral vector and vaccine safety profiles, this review provides insights on the likely presence of EVs in the final formulation of enveloped virus products and discusses the potential to further resolve and document these components.
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Woelk, Christopher H., and Edward C. Holmes. "Reduced Positive Selection in Vector-Borne RNA Viruses." Molecular Biology and Evolution 19, no. 12 (December 1, 2002): 2333–36. http://dx.doi.org/10.1093/oxfordjournals.molbev.a004059.
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Sena-Esteves, Miguel, and Guangping Gao. "Monitoring Lentivirus Vector Stocks for Replication-Competent Viruses." Cold Spring Harbor Protocols 2018, no. 4 (April 2018): pdb.prot095703. http://dx.doi.org/10.1101/pdb.prot095703.
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Jia, Dongsheng, Qian Chen, Qianzhuo Mao, Xiaofeng Zhang, Wei Wu, Hongyan Chen, Xiangzhen Yu, Zhiqiang Wang, and Taiyun Wei. "Vector mediated transmission of persistently transmitted plant viruses." Current Opinion in Virology 28 (February 2018): 127–32. http://dx.doi.org/10.1016/j.coviro.2017.12.004.
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François, Achille, Nicolas Eterradossi, Bernard Delmas, Vincent Payet, and Patrick Langlois. "Construction of Avian Adenovirus CELO Recombinants in Cosmids." Journal of Virology 75, no. 11 (June 1, 2001): 5288–301. http://dx.doi.org/10.1128/jvi.75.11.5288-5301.2001.
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ABSTRACT The avian adenovirus CELO is a promising vector for gene transfer applications. In order to study this potentiality, we developed an improved method for construction of adenovirus vectors in cosmids that was used to engineer the CELO genome. For all the recombinant viruses constructed by this method, the ability to produce infectious particles and the stability of the genome were evaluated in a chicken hepatocarcinoma cell line (LMH cell line). Our aim was to develop a replication-competent vector for vaccination of chickens, so we first generated knockout point mutations into 16 of the 22 unassigned CELO open reading frames (ORFs) to determine if they were essential for virus replication. As the 16 independent mutant viruses replicated in our cellular system, we constructed CELO genomes with various deletions in the regions of these nonessential ORFs. An expression cassette coding for the enhanced green fluorescent protein (eGFP) was inserted in place of these deletions to easily follow expression of the transgene and propagation of the vector in cell monolayers. Height-distinct GFP-expressing CELO vectors were produced that were all replication competent in our system. We then retained the vector backbone with the largest deletion (i.e., 3.6 kb) for the construction of vectors carrying cDNA encoding infectious bursal disease virus proteins. These CELO vectors could be useful for vaccination in the chicken species.
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Wintermantel, William M., Arturo A. Cortez, Amy G. Anchieta, Anju Gulati-Sakhuja, and Laura L. Hladky. "Co-Infection by Two Criniviruses Alters Accumulation of Each Virus in a Host-Specific Manner and Influences Efficiency of Virus Transmission." Phytopathology® 98, no. 12 (December 2008): 1340–45. http://dx.doi.org/10.1094/phyto-98-12-1340.
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Tomato chlorosis virus (ToCV), and Tomato infectious chlorosis virus (TICV), family Closteroviridae, genus Crinivirus, cause interveinal chlorosis, leaf brittleness, and limited necrotic flecking or bronzing on tomato leaves. Both viruses cause a decline in plant vigor and reduce fruit yield, and are emerging as serious production problems for field and greenhouse tomato growers in many parts of the world. The viruses have been found together in tomato, indicating that infection by one Crinivirus sp. does not prevent infection by a second. Transmission efficiency and virus persistence in the vector varies significantly among the four different whitefly vectors of ToCV; Bemisia tabaci biotypes A and B, Trialeurodes abutilonea, and T. vaporariorum. Only T. vaporariorum can transmit TICV. In order to elucidate the effects of co-infection on Crinivirus sp. accumulation and transmission efficiency, we established Physalis wrightii and Nicotiana benthamiana source plants, containing either TICV or ToCV alone or both viruses together. Vectors were allowed to feed separately on all virus sources, as well as virus-free plants, then were transferred to young plants of both host species. Plants were tested by quantitative reverse-transcription polymerase chain reaction, and results indicated host-specific differences in accumulation by TICV and ToCV and alteration of accumulation patterns during co-infection compared with single infection. In N. benthamiana, TICV titers increased during co-infection compared with levels in single infection, while ToCV titers decreased. However, in P. wrightii, titers of both TICV and ToCV decreased during mixed infection compared with single infection, although to different degrees. Vector transmission efficiency of both viruses corresponded with virus concentration in the host in both single and mixed infections. This illustrates that Crinivirus epidemiology is impacted not only by vector transmission specificity and incidence of hosts but also by interactions between viruses and efficiency of accumulation in host plants.
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Shabman, Reed S., Thomas E. Morrison, Christopher Moore, Laura White, Mehul S. Suthar, Linda Hueston, Nestor Rulli, et al. "Differential Induction of Type I Interferon Responses in Myeloid Dendritic Cells by Mosquito and Mammalian-Cell-Derived Alphaviruses." Journal of Virology 81, no. 1 (November 1, 2006): 237–47. http://dx.doi.org/10.1128/jvi.01590-06.
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ABSTRACT Dendritic cells (DCs) are an important early target cell for many mosquito-borne viruses, and in many cases mosquito-cell-derived arboviruses more efficiently infect DCs than viruses derived from mammalian cells. However, whether mosquito-cell-derived viruses differ from mammalian-cell-derived viruses in their ability to induce antiviral responses in the infected dendritic cell has not been evaluated. In this report, alphaviruses, which are mosquito-borne viruses that cause diseases ranging from encephalitis to arthritis, were used to determine whether viruses grown in mosquito cells differed from mammalian-cell-derived viruses in their ability to induce type I interferon (IFN) responses in infected primary dendritic cells. Consistent with previous results, mosquito-cell-derived Ross River virus (mos-RRV) and Venezuelan equine encephalitis virus (mos-VEE) exhibited enhanced infection of primary myeloid dendritic cells (mDCs) compared to mammalian-cell-derived virus preparations. However, unlike the mammalian-cell-derived viruses, which induced high levels of type I IFN in the infected mDC cultures, mos-RRV and mos-VEE were poor IFN inducers. Furthermore, the poor IFN induction by mos-RRV contributed to the enhanced infection of mDCs by mos-RRV. These results suggest that the viruses initially delivered by the mosquito vector differ from those generated in subsequent rounds of replication in the host, not just with respect to their ability to infect dendritic cells but also in their ability to induce or inhibit antiviral type I IFN responses. This difference may have an important impact on the mosquito-borne virus's ability to successfully make the transition from the arthropod vector to the vertebrate host.