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1

Rollins, Jull. "Urine collection pads." Paediatric Nursing 9, no. 7 (September 1997): 10. http://dx.doi.org/10.7748/paed.9.7.10.s19.

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HARRIS, PATRICIA. "Collection of urine." Equine Veterinary Journal 20, no. 2 (March 1988): 86–88. http://dx.doi.org/10.1111/j.2042-3306.1988.tb01465.x.

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3

Jan, Abiroo, and Akeela Fatima. "Urine Collection And Transport." JMS SKIMS 20, no. 1 (June 16, 2017): 52–53. http://dx.doi.org/10.33883/jms.v20i1.314.

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The most common urine specimen received is the per-urethral voided urine. Healthy urethra is unsterile and it is extremely critical that urine specimens be collected carefully to minimise urethral contamination. There are several types of urine specimens and the results of each type are determined by different guidelines. Therefore, it is essential that each urine specimen received by the laboratory is clearly labelled as to the type of collection of urine specimen. JMS 2017; 20(1):52-53
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4

Waddington, Philippa M., and Alan R. Watson. "Which urine collection bag?" Paediatric Nursing 9, no. 2 (March 1997): 19–20. http://dx.doi.org/10.7748/paed.9.2.19.s25.

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5

Vernon, Sue. "Urine collection pads overlooked." Paediatric Nursing 9, no. 5 (June 1997): 11. http://dx.doi.org/10.7748/paed.9.5.11.s16.

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6

&NA;. "URINE COLLECTION & CONTAMINATION." American Journal of Nursing 100, no. 12 (December 2000): 24DDD. http://dx.doi.org/10.1097/00000446-200012000-00030.

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7

Fellner, V., M. F. Weiss, A. T. Belo, R. L. Belyea, F. A. Martz, and A. H. Orma. "Urine Cup for Collection of Urine from Cows." Journal of Dairy Science 71, no. 8 (August 1988): 2250–55. http://dx.doi.org/10.3168/jds.s0022-0302(88)79800-8.

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8

Lemann, J., L. J. Hornick, J. A. Pleuss, and R. W. Gray. "Oxalate is overestimated in alkaline urines collected during administration of bicarbonate with no specimen pH adjustment." Clinical Chemistry 35, no. 10 (October 1, 1989): 2107–10. http://dx.doi.org/10.1093/clinchem/35.10.2107.

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Abstract We compared measurements of daily urine oxalate excretion in urines collected at the prevailing urine pH with measurements of urine oxalate excretion in urines collected into 20 mL of 6 mol/L HCl. We studied eight healthy adults fed constant diets. Urines were collected during control conditions and, in each subject, during the administration of NaCl, KCl, NaHCO3, or KHCO3, 90 mmol/day. Daily urine oxalate excretion calculated for collections made in acid averaged 271 (SD 79) mumol/day and did not vary with any of the salt supplements. When urines were collected at ambient urine pH (average 5.94, SD 0.23) during control conditions, and during the administration of NaCl or KCl, urine oxalate excretion averaged 263 (SD 88) mumol/day, a value not different from that for collections in acid. However, when urine was collected with no pH adjustment during NaHCO3 or KHCO3 administration (average pH 6.90, SD 0.14), apparent urine oxalate excretion averaged 398 (SD 132) mumol/day, significantly (P less than 0.025) exceeding the mean observed when urines were collected in acid. Moreover, the percentage increase in apparent oxalate excretion increased with urinary pH. These observations reinforce recommendations that urine specimens for measurement of oxalate be collected in acid to avoid the increase in apparent oxalate content that occurs during collection of alkaline urines. This increase presumably results from the well-known in vitro nonenzymatic conversion of ascorbate to oxalate.
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9

Wilson, Thomas, Isabel Garcia-Perez, Joram M. Posma, Amanda J. Lloyd, Edward S. Chambers, Kathleen Tailliart, Hassan Zubair, et al. "Spot and Cumulative Urine Samples Are Suitable Replacements for 24-Hour Urine Collections for Objective Measures of Dietary Exposure in Adults Using Metabolite Biomarkers." Journal of Nutrition 149, no. 10 (June 26, 2019): 1692–700. http://dx.doi.org/10.1093/jn/nxz138.

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ABSTRACT Background Measurement of multiple food intake exposure biomarkers in urine may offer an objective method for monitoring diet. The potential of spot and cumulative urine samples that have reduced burden on participants as replacements for 24-h urine collections has not been evaluated. Objective The aim of this study was to determine the utility of spot and cumulative urine samples for classifying the metabolic profiles of people according to dietary intake when compared with 24-h urine collections in a controlled dietary intervention study. Methods Nineteen healthy individuals (10 male, 9 female, aged 21–65 y, BMI 20–35 kg/m2) each consumed 4 distinctly different diets, each for 1 wk. Spot urine samples were collected ∼2 h post meals on 3 intervention days/wk. Cumulative urine samples were collected daily over 3 separate temporal periods. A 24-h urine collection was created by combining the 3 cumulative urine samples. Urine samples were analyzed with metabolite fingerprinting by both high-resolution flow infusion electrospray mass spectrometry (FIE-HRMS) and proton nuclear magnetic resonance spectroscopy (1H-NMR). Concentrations of dietary intake biomarkers were measured with liquid chromatography triple quadrupole mass spectrometry and by integration of 1H-NMR data. Results Cross-validation modeling with 1H-NMR and FIE-HRMS data demonstrated the power of spot and cumulative urine samples in predicting dietary patterns in 24-h urine collections. Particularly, there was no significant loss of information when post-dinner (PD) spot or overnight cumulative samples were substituted for 24-h urine collections (classification accuracies of 0.891 and 0.938, respectively). Quantitative analysis of urine samples also demonstrated the relation between PD spot samples and 24-h urines for dietary exposure biomarkers. Conclusions We conclude that PD spot urine samples are suitable replacements for 24-h urine collections. Alternatively, cumulative samples collected overnight predict similarly to 24-h urine samples and have a lower collection burden for participants.
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10

MATSUMOTO, kazuhiko, Toyohiko MORINO, Hitomi HARA, and keiko MOTOYAMA. "Collection and examination of urine." Journal of Toxicological Sciences 11, no. 4 (1986): 404–7. http://dx.doi.org/10.2131/jts.11.404.

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11

Mcgoldrick, Mary. "Urine Specimen Collection and Transport." Home Healthcare Now 33, no. 5 (May 2015): 284–85. http://dx.doi.org/10.1097/nhh.0000000000000233.

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12

HELLERSTEIN, S. "Urine collection for protein excretion." Journal of Pediatrics 144, no. 6 (June 2004): 834–35. http://dx.doi.org/10.1016/s0022-3476(04)00171-4.

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13

Vernon, S., A. Redfearn, S. J. Pedler, H. J. Lambert, and M. G. Coulthard. "Urine collection on sanitary towels." Lancet 344, no. 8922 (August 1994): 612. http://dx.doi.org/10.1016/s0140-6736(94)91996-8.

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14

Sovell, Paul J. "Urine collection from disposable nappies." Annals of Emergency Medicine 21, no. 2 (February 1992): 233–34. http://dx.doi.org/10.1016/s0196-0644(05)80183-0.

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15

Ahmad, T., D. Vickers, S. Campbell, M. G. Coulthard, and S. Pedler. "Urine collection from disposable nappies." Lancet 338, no. 8768 (September 1991): 674–76. http://dx.doi.org/10.1016/0140-6736(91)91242-m.

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16

Björklund, L. J., Vitas Dargis, Robert Chen, David Brown, Giancarlo Viberti, Harry Keen, James Walker, Nigel Masters, and I. Blumenthal. "Urine collection from disposable nappies." Lancet 338, no. 8774 (October 1991): 1077–78. http://dx.doi.org/10.1016/0140-6736(91)91933-l.

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17

Cheek, John A., Simon S. Craig, Robert W. Seith, and Adam West. "Urine collection in young children." Emergency Medicine Australasia 27, no. 4 (June 26, 2015): 348–50. http://dx.doi.org/10.1111/1742-6723.12442.

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18

May, Olivia Windham. "Urine Collection Methods in Children." Nursing Clinics of North America 53, no. 2 (June 2018): 137–43. http://dx.doi.org/10.1016/j.cnur.2018.01.001.

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19

Calanchini, Matilde, Michael Tadman, Jesper Krogh, Andrea Fabbri, Ashley Grossman, and Brian Shine. "Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection." Endocrine Connections 8, no. 8 (August 2019): 1082–88. http://dx.doi.org/10.1530/ec-19-0269.

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Background The 24-h urinary output of 5-hydroxyindoleacetic acid (5-HIAA) is used to monitor disease progression and treatment responses of neuroendocrine neoplasms (NENs). Several conditions are required for 5-HIAA assay, involving urine collection/preservation and food/drug restrictions. Aim To evaluate the correlation between 5-HIAA concentration in a spot urine sample and the output in a 24-h urine collection, and whether spot urine specimens can replace 24-h collection. Methods Patients with NENs or symptoms suggestive of NENs were asked to provide a separate spot urine at the end of the 24-h urine collection for 5-HIAA assessment. The upper reference limit for 24-h urinary 5-HIAA was 40 µmol/24 h. 5-HIAA measurements in spot urine samples were corrected for variation in urine flow rate by expressing results as a ratio to creatinine concentration. Results We included 136 paired urinary samples for 5-HIAA assessment from 111 patients (100 NENs). The correlation between 5-HIAA values measured in 24-h and spot urines was r = +0.863 (P < 0.001) and r = +0.840 (P < 0.001) including only NEN patients. Using the 24-h urinary 5-HIAA as reference method, the AUC on ROC analysis for spot urinary 5-HIAA was 0.948 (95% CI, 0.914–0.983; P < 0.001), attaining a sensitivity of 83% and specificity of 95% using 5.3 mol/mmol as cut-off for the spot urine. The AUC among NEN patients alone was 0.945 (95% CI, 0.904–0.987; P < 0.001). Conclusions The ratio of 5-HIAA to creatinine in a spot urine could replace the measurement of 5-HIAA output in a 24-h urine collection, especially for follow-up of patients with known elevated 5-HIAA levels.
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20

Mladina, Nada, Devleta Hadzic, Elvira Konjic, and Zarko Mladina. "EFFECT OF URINE SAMPLE COLLECTION METHOD ON CONTAMINATION RATE OF URINE CULTURE." Acta Medica Saliniana 38, no. 1 (February 3, 2009): 36–40. http://dx.doi.org/10.5457/ams.v38i1.29.

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Introduction: Urine sample for biochemical analysis must fulfill certain criterions. The sample collection must be done by following established standards so that the results of analysis are reliable. In children of various age, especially during serious disease, adequate consideration must be devoted to this procedure.Aims: To evaluate contamination rate of the urine sample according to the methods of obtaining samples and collecting specimens in seriously sick children of various age during their intensive treatment.Methods: Urine culture findings in children treated in Intensive Care Unit (ICU) of Children’s Hospital in Tuzla in period from January 2007 to the end of December 2007 were included in retrospective analysis according to the method of collecting (bag collection, urethral catheterization, clean catch). In all of the three groups the percentage of positive findings and percentage of contaminated specimens as well as sex related distribution was analyzed. The urine sample was obtained from urethral catheter only in patients with indication for urethral catheterization. Kruskal-Wallis test and regression model were used in statistical analysis. Results: A total of 662 children were treated in ICU during the observed period. The urine sample for routine biochemical tests was obtained from all patients. In 107 patients (16.2 %) urine culture examination was indicated. In 48 (44.9%) patients urine sample was obtained by bag collection, in 41 (38.3%) by clean catch, and 18 (16.8%) by urethral catheterization. In 7 patients or 6.5% urine was contaminated. The majority of contaminated specimens were collected by bag (12.5%). In 20 (18.7%) patients urine culture was positive with significant number of etiologic agents and 80 (74.8%) specimens were negative. Difference in results in three monitored groups was statistically significant which was confirmed by Kruskal-Wallis test and stepwise regression model.Conclusion: Obtaining urine sample by bag collection brings the highest risk for contamination.
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21

Al-Attar, Laila, Wafaa Bakr, and Randa Abd Al-Aleem. "Do Urine Collection Techniques Affect Contamination?" Journal of High Institute of Public Health 35, no. 1 (January 1, 2005): 55–66. http://dx.doi.org/10.21608/jhiph.2005.179521.

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22

Kurien, Biji T., Nancy E. Everds, and R. Hal Scofield. "Experimental animal urine collection: a review." Laboratory Animals 38, no. 4 (October 2004): 333–61. http://dx.doi.org/10.1258/0023677041958945.

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23

Chew, Joon Lin, and Kaw Yan Chua. "Collection of Mouse Urine for Bioassays." Lab Animal 32, no. 7 (August 2003): 48–50. http://dx.doi.org/10.1038/laban0803-48.

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24

Boudra, H., P. Noziere, G. Cantalapiedra-Hijar, M. Traikia, J.-F. Martin, M. Petera, M. Lagree, M. Doreau, and D. P. Morgavi. "Spot urine collection: A valid alternative to total urine collection for metabolomic studies in dairy cattle." Journal of Dairy Science 105, no. 1 (January 2022): 301–12. http://dx.doi.org/10.3168/jds.2021-20788.

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25

Kaplan, Jennifer S., and Gary L. Horowitz. "Twenty-Four–Hour Bence-Jones Protein Determinations: Can We Ensure Accuracy?" Archives of Pathology & Laboratory Medicine 135, no. 8 (August 1, 2011): 1048–51. http://dx.doi.org/10.5858/2010-0547-oar.

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Context.—Light chain disease represents 15% to 20% of cases of multiple myeloma. Current guidelines recommend monitoring these patients with 24-hour urine collections. Objective.—To determine the reliability of 24-hour urine collections in assessing the amount of Bence-Jones protein (BJP). Design.—We included all patients from our institution from 2003 through 2008 with BJP who had more than four 24-hour urine collections. We compared BJP excretion calculated from the submitted 24-hour collection with BJP excretion calculated by normalizing the collection to that patient's mean 24-hour creatinine excretion. We also looked at differences in serial values with these 2 methods. In addition, we evaluated the feasibility of using random urine samples to determine BJP excretion. Results.—A total of 14 patients with 135 24-hour urine collections met our inclusion criteria. The 24-hour urine creatinine excretion for each patient, which should be reasonably constant, varied considerably (coefficient of variation range 12%–30%). Differences in the 2 methods of calculating BJP excretion ranged from −1588 to 2315 mg/d. Among a total of 121 serial 24-hour measurements, the differences were clinically significant in 37 (30%). Among a total of 23 random urine samples from 11 of these patients submitted within 10 days of a 24-hour collection, the estimated BJP excretion appeared to be accurate in at least 18 (78%). Conclusions.—Twenty-four–hour urine collections for BJP are, in practice, often misleading. At a minimum, one should verify that the 24-hour creatinine excretion is accurate. In addition, it may be possible to use the protein/creatinine ratio from random urine samples to determine 24-hour BJP excretion.
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Kaufman, Jonathan, Andrew Joshua Knight, Penelope A. Bryant, Franz E. Babl, and Kim Dalziel. "Liquid gold: the cost-effectiveness of urine sample collection methods for young precontinent children." Archives of Disease in Childhood 105, no. 3 (August 23, 2019): 253–59. http://dx.doi.org/10.1136/archdischild-2019-317561.

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BackgroundUrinary tract infection (UTI) is a common childhood infection. Many febrile children require a urine sample to diagnose or exclude UTI. Collecting urine from young children can be time-consuming, unsuccessful or contaminated. Cost-effectiveness of each collection method in the emergency department is unknown.ObjectiveTo determine the cost-effectiveness of urine collection methods for precontinent children.MethodsA cost-effectiveness analysis was conducted comparing non-invasive (urine bag, clean catch and 5 min voiding stimulation for clean catch) and invasive (catheterisation and suprapubic aspirate (SPA)) collection methods, for children aged 0–24 months in the emergency department. Costs included equipment, staff time and hospital bed occupancy. If initial collection attempts were unsuccessful subsequent collection using catheterisation was assumed. The final outcome was a definitive sample incorporating progressive dipstick, culture and contamination results. Average costs and outcomes were calculated for initial collection attempts and obtaining a definitive sample. One-way and probabilistic sensitivity analyses were performed.ResultsFor initial collection attempts, catheterisation had the lowest cost per successful collection (GBP£25.98) compared with SPA (£37.80), voiding stimulation (£41.32), clean catch (£52.84) and urine bag (£92.60). For definitive collection, catheterisation had the lowest cost per definitive sample (£49.39) compared with SPA (£51.84), voiding stimulation (£52.25), clean catch (£64.82) and urine bag (£112.28). Time occupying a hospital bed was the most significant determinant of cost.ConclusionCatheterisation is the most cost-effective urine collection method, and voiding stimulation is the most cost-effective non-invasive method. Urine bags are the most expensive method. Although clinical factors influence choice of method, considering cost-effectiveness for this common procedure has potential for significant aggregate savings.
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Nabavizadeh, Pooneh, Shadi Ghadermarzi, and Mohammad Fakhri. "A New Method to Make 24-Hour Urine Collection More Convenient: A Validity Study." International Journal of Nephrology 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/718147.

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Background and Objectives. This study proposes a novel urine collection device that can divide each urine collection into 20 parts and store and cool just one part. The aim of the current study is to compare measured biomarkers from the proposed urine collection device to those of conventional 24-hour sampling method. We also hypothesized that the new method would significantly increase patients’ adherence to the timed urine collection.Methods. Two 24-hour urine samples with the conventional method and with the new automated urine collection device that uses just one-twentieth of each void were obtained from 40 healthy volunteers. Urine parameters including volume, creatinine, and protein levels were compared between the two methods and the agreement of two measurements for each subject was reported through Bland-Altman plots.Results. Our results confirmed that for all three variables, there is a positive correlationP<0.001between the two measurements and high degree of agreement could be seen in Bland-Altman plots. Moreover, more subjects reported the new method as “more convenient” for 24-hour urine collection.Conclusions. Our results clearly indicate that a fixed proportion of each void may significantly reduce the urine volume in timed collections and this, in turn, may increase subjects’ adherence to this difficult sampling.
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Bethune, P., J. A. Mills, and G. J. German. "Rapid, Non-Invasive Urine Collection is Feasible in Infants Up To 12 Months Old." Paediatrics & Child Health 21, Supplement_5 (June 1, 2016): e54a-e54a. http://dx.doi.org/10.1093/pch/21.supp5.e54a.

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Abstract BACKGROUND: In non-toilet trained children, collection of non-contaminated urine specimens for culture is done by urethral catheterization or suprapubic aspiration,both invasive procedures, or by clean-catch technique, which is time-consuming. A rapid, non-invasive method of urine collection from neonates has been recently described:a stimulated clean-catch technique, with an 87% success rate within 5 minutes (Herreros et al, Arch Dis Child 2013; 98:27-29). OBJECTIVES: To determine: 1) the success rate in obtaining urine specimens within 5 minutes from infants (birth-12 months) via this stimulated clean-catch method; and 2) the time to collection of a urine specimen, via the stimulated clean-catch method. DESIGN/METHODS: Subjects were consecutive infants requiring a urine specimen for urinalysis and/or urine culture, identified by nursing or medical staff in either the inpatient or outpatient areas of a regional hospital. Verbal consent was obtained from the parent or guardian. 10- 60 minutes after a feeding, the infant’s genitalia were cleaned, and a stimulated clean-catch urine collection was attempted. The method consists of holding the infant under the axillae, with legs dangling, and stimulating voiding by rapidly tapping the suprapubic area for 30 seconds, then gently massaging in a circular pattern over the paralumbar area bilaterally for 30 seconds. The tapping and massage are repeated alternately until the infant voids, and the urine is collected “mid-stream”. RESULTS: Urine collection was attempted 51times, from 43infants, and was successful within 5 minutes in 37of 51attempts (72%). In infants 0-30 days old, the success rate was 64% (14 of 22 attempts). In infants 31-90 days old, the success rate was 93% (14 of 15 attempts). In infants 3-6 months old, the success rate was 67% (4 of 6 attempts). In infants 6-12 months old, the success rate was 63% (5 of 8attempts). The time to successful urine collection was recorded in 26cases, with both a median and a mean time of 85seconds. In 77% of the successful collections, the urine was obtained within 2 minutes. CONCLUSION: The stimulated clean-catch method of urine collection is useful in infants up to 1 year of age. In most cases of successful urine collection, the infant will void within 2 minutes.
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Sonuch, Pitchaporn, Surasak Kantachuvesiri, Prin Vathesatogkit, Raweewan Lappichetpaiboon, Worawan Chailimpamontri, Nintita Sripaiboonkij Thokanit, and Wichai Aekplakorn. "Estimation of sodium consumption by novel formulas derived from random spot and 12-hour urine collection." PLOS ONE 16, no. 12 (December 2, 2021): e0260408. http://dx.doi.org/10.1371/journal.pone.0260408.

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The gold standard for estimating sodium intake is 24h urine sodium excretion. Several equations have been used to estimate 24h urine sodium excretion, however, a validated formula for calculating 24h urine sodium excretion from 12h urine collection has not yet been established. This study aims to develop novel equations for estimating 24h urine sodium excretion from 12h and random spot urine collection and also to validate existing spot urine equations in the Thai population. A cross-sectional survey was carried out among 209 adult hospital personnel. Participants were asked to perform a 12h daytime, 12h nighttime, and a random spot urine collection over a period of 24 hours. The mean 24h urine sodium excretion was 4,055±1,712 mg/day. Estimated urine sodium excretion from 3 different equations using random spot urine collection showed moderate correlation and agreement with actual 24h urine sodium excretion (r = 0.54, P<0.001, ICC = 0.53 for Kawasaki; r = 0.57, P<0.001, ICC = 0.44 for Tanaka; r = 0.60, P<0.001, ICC = 0.45 for INTERSALT). Novel equations for predicting 24h urine sodium excretion were then developed using variables derived from 12h daytime urine collection, 12h nighttime urine collection, random spot urine collection, 12h daytime with random spot urine collection, and 12h nighttime with random spot urine collection which showed strong correlation and agreement with actual measured values (r = 0.88, P<0.001, ICC = 0.87; r = 0.83, P<0.001, ICC = 0.81; r = 0.67, P<0.001, ICC = 0.62; r = 0.90, P<0.001, ICC = 0.90; and r = 0.83, p<0.001, ICC = 0.82 respectively). Bland-Altman plots indicated good agreement between predicted values and actual 24h urine sodium excretion using the new equations. Newly derived equations from 12h daytime and 12h nighttime urine collection with or without casual spot urine collection were able to accurately predict 24h urine sodium excretion.
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Macfarlane, Peter I., Robert Ellis, Christopher Hughes, Christine Houghton, and Robert Lord. "Urine collection pads: are samples reliable for urine biochemistry and microscopy?" Pediatric Nephrology 20, no. 2 (December 28, 2004): 170–79. http://dx.doi.org/10.1007/s00467-004-1709-4.

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31

Nagano, Nobuhiko, Takayuki Imaizumi, Takuya Akimoto, Midori Hijikata, Ryoji Aoki, Ayako Seimiya, Aya Okahashi, et al. "Clinical Evaluation of a Novel Urine Collection Kit Using Filter Paper in Neonates: An Observational Study." Children 8, no. 7 (June 29, 2021): 561. http://dx.doi.org/10.3390/children8070561.

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Urine bags are commonly used to collect urine samples from neonates. However, the sample can be contaminated by stool, or detachment of the bag due to body movement can lead to failure of the collection. A qualitative urine collection kit containing ten filter papers of 3.2 mm diameter was developed and clinically verified among 138 neonates. During a single diaper change (approximately 3 h), the rate of urine collection was calculated. Urine collection was considered to be successful if any filter paper in the urine collection sheet turned from blue to white. Of the 127 neonates who passed urine, 122 had a change in the filter paper. The urine collection rate was 96%, with changes in all 10 filter papers observed in 98 neonates (80%). Urine collection rate was not influenced by sex (p = 1.00), age at collection (p = 0.72), preterm birth (p = 1.00), low birth weight (p = 0.92), or fecal contamination (p = 1.00). The incidence of dermatitis was not higher than in the group in which urine bags were used (urine collection kit: 2/68 [3%]; urine bag: 5/68 [7%]; p = 0.44). Novel urine collection kits using filter paper can collect samples from neonates safely and with a high probability of success.
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Susmitha, Apuri, Taiga Nishihori, Karen Lin, Rachid Baz, Binglin Yue, Jongphil Kim, Kenneth H. Shain, et al. "A Strong Correlation Between Spot Urine Protein To Creatinine Ratio and 24-Hour Urine Protein Quantification For Proteinuria Assessment In Patients With Plasma Cell Dyscrasia." Blood 122, no. 21 (November 15, 2013): 3155. http://dx.doi.org/10.1182/blood.v122.21.3155.3155.

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Abstract Background Twenty four-hour urine collection remains one of the most crucial tools for the diagnosis and monitoring of proteinuria and quantification of urinary monoclonal protein in patients with plasma cell dyscrasias (PCD) even though it is cumbersome. Nephrology guidelines recommend replacement of 24-hour urine collection with a spot urine protein/creatinine (Pr/Cr) ratio for the measurement of proteinuria. However, only limited data exist regarding accuracy of spot urine Pr/Cr ratio in patients with PCD and utility of this measure to estimate urinary paraprotein remains uncertain. We conducted a prospective study evaluating the role of spot urine Pr/Cr ratio in patients with PCD. Methods From 04/2012 to 05/2013, a total of 120 PCD patients were prospectively enrolled at Moffitt Cancer Center. Oliguric patients or those requiring dialysis were ineligible. Spot urine was collected on the same day as 24-hour urine collection. Spot urine Pr/Cr ratios were compared to 24-hour urine collections with regard to the amount of (1) total protein and (2) monoclonal protein (M-spike). Results Eighty four out of 120 patients (70%) were evaluable (17 did not provide spot urine samples; no Pr/Cr ratios available in 11; protein below the level of detection in 7; and one without 24-hour urine collection). Evaluable patients had a median age of 68 (range, 36 - 90) years, 63% were male, and 85% were Caucasian. Primary diagnoses were myeloma (n=74; 88%), amyloidosis (n=4), multiple plasmacytomas (n=2), solitary plasmactyoma (n=1) and MGUS (n=3). Prior therapies included chemotherapy in 95% and autologous transplant in 45%. Comorbidities included hypertension (48%), chronic kidney disease (30%), diabetes (15%), coronary artery disease (8%), atrial fibrillation (7%) and congestive heart failure (2%). The median serum creatinine was 0.9 mg/dL (range, 0.5 - 5.1). The median spot urine Pr/Cr ratio was 137 mg/g creatinine (range, 26 - 21,447). The median 24-hour urine protein was 120 mg/24h (range, 27 - 15,092), and the median urine M-spike was 1.2 mg (range, 0 - 8,099). More than half of spot urine samples were collected in the morning (59%). There were strong correlations between (1) spot urine Pr/Cr ratio and 24-hour total urine protein (Spearman correlation coefficient=0.91, p < 0.0001), and (2) spot urine Pr/Cr ratio and 24-hour urine M-spike (Spearman correlation coefficient=0.91, p < 0.0001). The timing of spot urine sample collection had no significant effects on the correlations (p = 0.46 and 0.95 by Wilcoxon rank-sum test). Conclusions Spot urine Pr/Cr ratio strongly correlates with degrees of proteinuria measured in 24-hour urine collection and may also predict the quantity of urine M-spike in non-oliguric PCD population. Spot urine Pr/Cr ratio is a potentially useful marker as a screening tool or an alternative semi-quantitative measure for rapid estimation of proteinuria which may be used for longitudinal patient follow-up in lieu of cumbersome repeated 24-hour collections. Disclosures: No relevant conflicts of interest to declare.
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Lascano, G. J., G. I. Zanton, A. J. Heinrichs, and W. P. Weiss. "Technical note: A noninvasive urine collection device for female cattle: Modification of the urine cup collection method." Journal of Dairy Science 93, no. 6 (June 2010): 2691–94. http://dx.doi.org/10.3168/jds.2009-3027.

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34

Evans, Wayne, Jon P. Lensmeyer, Russell S. Kirby, Margaret E. Malnory, and Fredrik F. Broekhuizen. "Two-hour urine collection for evaluating renal function correlates with 24-hour urine collection in pregnant patients." Journal of Maternal-Fetal and Neonatal Medicine 9, no. 4 (January 2000): 233–37. http://dx.doi.org/10.3109/14767050009020538.

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35

Pattyn, Jade, Severien Van Keer, Samantha Biesmans, Margareta Ieven, Charlotte Vanderborght, Koen Beyers, Vanessa Vankerckhoven, Robin Bruyndonckx, Pierre Van Damme, and Alex Vorsters. "Human papillomavirus detection in urine: Effect of a first-void urine collection device and timing of collection." Journal of Virological Methods 264 (February 2019): 23–30. http://dx.doi.org/10.1016/j.jviromet.2018.11.008.

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36

Evans, Wayne, Jon P. Lensmeyer, Russell S. Kirby, Margaret E. Malnory, and Fredrik F. Broekhuizen. "Two-Hour Urine Collection for Evaluating Renal Function Correlates With 24-Hour Urine Collection in Pregnant Patients." Obstetric and Gynecologic Survey 56, no. 3 (March 2001): 131–32. http://dx.doi.org/10.1097/00006254-200103000-00006.

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37

Evans, Wayne, Jon P. Lensmeyer, Russell S. Kirby, Margaret E. Malnory, and Fredrik F. Broekhuizen. "Two-hour urine collection for evaluating renal function correlates with 24-hour urine collection in pregnant patients." Journal of Maternal-Fetal Medicine 9, no. 4 (2000): 233–37. http://dx.doi.org/10.1002/1520-6661(200007/08)9:4<233::aid-mfm9>3.0.co;2-s.

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38

PATIENCE, J. F., D. W. FRIEND, K. E. HARTIN, and M. S. WOLYNETZ. "A COMPARISON OF TWO URINE COLLECTION METHODS FOR FEMALE SWINE." Canadian Journal of Animal Science 67, no. 3 (September 1, 1987): 859–63. http://dx.doi.org/10.4141/cjas87-089.

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Six pairs of littermate gilts were used to compare an invasive (catheter) and a noninvasive (tube affixed around the vagina) urine collection system. Urine volume and nitrogen excretion were greater and water retention and urine specific gravity less (P < 0.05) in the catheterized swine. Collection method had no effect on urogenital infection. Key words: Urine collection, catheter, urine composition, swine
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39

Shingfield, K. J., and N. W. Offer. "Evaluation of the spot urine sampling technique to assess urinary purine derivative excretion in lactating dairy cows." Animal Science 66, no. 3 (June 1998): 557–68. http://dx.doi.org/10.1017/s1357729800009139.

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AbstractThe potential of the spot urine sampling technique as an alternative to performing a total urine collection was evaluated. Twelve multiparous Holstein-Friesian cows were given two experimental diets in a complete change-over design using two 14-day experimental periods. Experimental diets were either silage offeredad libitumwith 7 kg fresh weight concentrate supplement as a single meal (SF), or a complete diet formulated from the same ingredients with a similar foragexoncentrate ratio (CD). Total urine collections were performed every 2 h on days 11 and 14 of each experimental period. Subsamples of urine were stored at 20°C and subsequently analysed by high-performace liquid chromatography. Daily allantoin and purine derivative (PD) excretion were highly correlated (r = 0·995, no. = 48,P< 0·001). PD and creatinine excretion during each 2-h interval depended on time of collection (PD,P< 0·001 and creatinine,P< 0·05) and on cow (P< 0·01) but were unaffected by sampling day or treatment. Diurnal variations in the molar ratio ofPD or allantoin to creatinine (PD/c and Ale, respectively) followed similar diurnal patterns as observed for PD and allantoin excretion. The data were used to assess the error of prediction of daily mean PD/c or Ale ratios. Three spot sampling regimens (based on the collection of four 4-h samples, three 8-h samples or two 12-h samples) and also on either single or 2-day urine collections were evaluated. Collection of multiple samples within a day was more reliable than collecting fewer samples over several days. Prediction errors were greater for SF compared with CD. Even the most intensive sampling regimen did not allow an acceptable prediction of daily mean PDIc or Ale ratio, minimum r values for PDIc and Ale ratios were 0·098, 0·136 and 0·547, 0·579 for SF and CD, respectively. Furthermore, daily mean PDIc and Ale ratios proved poor predictors of daily PD and allantoin excretion (r values of 0·69 and 0·72, respectively). Total urine collection appears necessary to assess accurately daily PD excretion in dairy cows.
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Nepal, Archana, Rydam Basnet, Rukma Acharya, Sushma Shrestha, Satish Koirala, Anshu Poudel, Anil Ojha, and Dharma Sharna. "A technique for fast and safe collection of urine in newborns." Journal of Kathmandu Medical College 5, no. 3 (October 16, 2017): 82–84. http://dx.doi.org/10.3126/jkmc.v5i3.18419.

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Background: Urine collection in children especially in new born is a great challenge. A wide range of clinical interventions for urine collection is described in the literature, including non-invasive and invasive methods. Mid-stream urine collection is considered the most appropriate technique for older children. Here we are testing a method for obtaining mid- stream urine sample in newborns.Objective: To test a technique of urine collection for obtaining mid-stream urine sample in newborns.Method: A prospective feasibility study of a technique of urine sample collection based on bladder and lumbar stimulation maneuvers done in 100 newborns of less than 28 days of life over a period of one month. The main variable was the success rate in obtaining a midstream urine sample collection within four minutes and secondary variables were time required to obtain the sample and associated complications.Results: Mid- stream urine sample was collected successfully in 91% of babies. Mean time required for urine collection was 59.7 seconds with standard deviation of 46.4 seconds and median time was 47 seconds. No untoward complication other than controlled crying was seen.Conclusion: This is a quick and safe technique for mid-stream urine sample collection in newborn with high success rate and minimal discomfort.Journal of Kathmandu Medical CollegeVol. 5, No. 3, Issue 17, Jul.-Sep., 2016, Page: 82-84
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41

Bertrand, P. V., B. T. Rudd, P. H. Weller, and A. J. Day. "Free cortisol and creatinine in urine of healthy children." Clinical Chemistry 33, no. 11 (November 1, 1987): 2047–51. http://dx.doi.org/10.1093/clinchem/33.11.2047.

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Abstract Cortisol and creatinine were measured in two consecutive overnight urine collections from 103 healthy school children, ages seven to 18.5 years. Mean cortisol and creatinine concentrations were respectively 194 nmol/L and 12.7 mmol/L. The data were statistically analyzed to simultaneously assess any effects of sex, age, weight, day of collection, and urine volume. Mean urine volume for boys exceeded that for girls, increased with body weight, and was greater on the second day of collection than on the first. Cortisol concentration was independent of sex, age, and weight, but decreased with urine volume. Boys excreted more cortisol than did girls, and the amount increased with urine volume. Creatinine concentration increased with weight, decreased with urine volume. Total creatinine increased with weight, was greater for boys than girls, and increased with urine volume. The cortisol/creatinine ratio was valueless as an index of adrenocortical status.
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42

Perera, CU. "Collection and transport of urine for culture." Ceylon Medical Journal 46, no. 2 (January 30, 2014): 77. http://dx.doi.org/10.4038/cmj.v46i2.6512.

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43

Rn, Sue vernon. "Urine collection from infants: a reliable method." Paediatric Nursing 7, no. 6 (July 1995): 26–27. http://dx.doi.org/10.7748/paed.7.6.26.s24.

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44

Saitoh, Hiroshi. "DESCRIPTIONS OF URINE IN THE HIPPOCRATIC COLLECTION." Japanese Journal of Urology 97, no. 1 (2006): 10–19. http://dx.doi.org/10.5980/jpnjurol1989.97.10.

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45

FELTON, R., A. GORDON, J. H. PALMER, and G. R. MUFTI. "Urostomy Specimen of Urine-Technique of Collection." British Journal of Urology 72, no. 2 (August 1993): 255–56. http://dx.doi.org/10.1111/j.1464-410x.1993.tb00705.x.

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46

Needleman, Saul B., Martin Porvaznik, and Dave Ander. "Creatinine Analysis in Single Collection Urine Specimens." Journal of Forensic Sciences 37, no. 4 (July 1, 1992): 13298J. http://dx.doi.org/10.1520/jfs13298j.

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47

Pieper, Barbara, and Virginia Cleland. "An External Urine-Collection Device for Women." Journal of Wound, Ostomy and Continence Nursing 20, no. 2 (March 1993): 51–55. http://dx.doi.org/10.1097/00152192-199303000-00010.

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Rees, JC, S. Vernon, SJ Pedler, and MG Coulthard. "Collection of urine from washed-up potties." Lancet 348, no. 9021 (July 1996): 197. http://dx.doi.org/10.1016/s0140-6736(05)66144-2.

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49

Tran, Antoine. "The Quick-Wee infant urine collection method." Journal of Pediatrics 188 (September 2017): 308–11. http://dx.doi.org/10.1016/j.jpeds.2017.06.059.

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Kurien, Biji T., and R. Hal Scofield. "Mouse urine collection using clear plastic wrap." Laboratory Animals 33, no. 1 (January 1999): 83–86. http://dx.doi.org/10.1258/002367799780578525.

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