Journal articles: 'Urine analysi'

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Örd, Lenna, Toomas Marandi, Marit Märk, Leonid Raidjuk, Jelena Kostjuk, Valdas Banys, Karit Krause, and Marika Pikta. "Evaluation of DOAC Dipstick Test for Detecting Direct Oral Anticoagulants in Urine Compared with a Clinically Relevant Plasma Threshold Concentration." Clinical and Applied Thrombosis/Hemostasis 28 (January 2022): 107602962210843. http://dx.doi.org/10.1177/10760296221084307.

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Measuring direct oral anticoagulant (DOAC) concentrations might be necessary in certain clinical situations but is not routinely performed. The DOAC Dipstick is a new rapid test for detecting DOACs in urine. The aim of this study was to evaluate the possible uses and limitations of the DOAC Dipstick and to compare visual analysis and DOASENSE Reader analysis of DOAC Dipstick pads. Plasma and urine samples were collected from 23 patients taking DOACs. DOAC concentrations in plasma and urine were measured by chromogenic substrate assays and in urine also by the DOAC Dipstick. Plasma concentrations were dichotomized at a threshold of ≥30 ng/mL. Patient samples were compared with samples from control individuals not using anticoagulants (n = 10) and with DOASENSE control urines. The Combur-10 test was used to measure parameters that may affect urine color and hence the interpretation of the DOAC Dipstick result. DOAC Dipstick test results were positive in 21/23 patient urine samples at a plasma DOAC concentration of ≥30 ng/mL and in 2/23 patient urine samples at a plasma DOAC concentration of <30 ng/mL. Inter-observer agreement was above 90% for visual analysis of patient urine samples and was 100% for DOASENSE Reader analysis of patient urines and for analysis of control group urines and DOASENSE control urines. Abnormalities in urine color detected by the Combur-10 test did not affect the DOAC Dipstick results. DOAC Dipstick detects DOACs in urine at a plasma threshold of ≥30 ng/mL. Positive DOAC Dipstick results should be confirmed by measuring DOAC plasma concentration.

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Clark, D. R., and T. M. Hajar. "Detection and confirmation of cocaine use by chromatographic analysis for methylecgonine in urine." Clinical Chemistry 33, no. 1 (January 1, 1987): 118–19. http://dx.doi.org/10.1093/clinchem/33.1.118.

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Abstract Methylecgonine is a common metabolite of cocaine in man. We prepared methylecgonine and developed thin-layer chromatographic and gas-chromatographic methods for its detection in urine. Seventy urine specimens from our drug screening laboratory were tested by our method and by EMIT. Both methods were positive for 26 urines, and both were negative for 42 urines. The other two urines were shown to contain cocaine by GC/MS, and no detectable metabolites. We thus demonstrated that detection of methylecgonine and cocaine is as sensitive a test for cocaine use as EMIT.

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Juhariah, Jujuk, and Margaretha Praba Aulia. "Analisis Pertumbuhan Tanaman Cabai Keriting dalam Polybag menggunakan Pupuk Fermentasi Urin Sapi." METANA 17, no. 2 (November 16, 2021): 49–54. http://dx.doi.org/10.14710/metana.v17i2.42565.

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Tahun 2020 merupakan tahun yang cukup sulit bagi masyarakat Indonesia. Adanya virus corona jenis baru memaksa masyarakat untuk beradaptasi dengan kebiasaan baru. Salah satu masalah terbesar yang dihadapi adalah dengan adanya kebijakan lockdown yang menyebabkan sulitnya distribusi bahan pangan. Oleh sebab itu edukasi masyarakat untuk memanfaatkan lahan pekarangan secara organik dengan mengoptimalkan sumber daya yang ada disekitar pekarangan rumah perlu dilakukan. Tujuan penelitian ini adalah untuk mengetahui respon tanaman cabai keriting dengan menggunakan pupuk fermentasi urin sapi. Penelitian dilakukan dengan cara memberikan perlakuan variasi pemupukan dengan mencampur urin sapi dan EM4 (perlakuan A); urin sapi, EM4, dan batang pohon pisang (perlakuan B); urin sapi, EM4, dan sabut kelapa (perlakuan C); dan urin sapi, EM4, dan akar kacang tanah (perlakuan D). Parameter yang diamati dalam penelitian ini adalah tinggi tanaman, jumlah daun, diameter batang, dan bobot biomassa kering tanaman. Perlakuan penambahan sabut kelapa pada fermentasi urin sapi memberikan pengaruh yang nyata pada parameter tinggi tanaman. Sedangkan penambahan akar kacang tanah pada fermentasi pupuk urin sapi meningkatkan bobot biomassa kering tanaman secara signifikan. Penambahan batang pohon pisang pada fermentasi urin sapi secara nyata memberikan pengaruh terhadap diameter batang tanaman cabai keriting. Akan tetapi, jumlah daun tidak menunjukkan perbedaan yang signifikan dari semua jenis pemupukan. The year 2020 is quite a difficult year for the people of Indonesia. The existence of a new coronavirus type forces people to adapt to new habits. One of the biggest problems faced is the lockdown policy which makes it difficult for food distribution. Therefore, it is necessary to educate the public to utilize the yard organically by optimizing the existing resources around the yard of the house. This study aimed to determine the response of curly chili plants using cow urine fermentation fertilizer. The research was conducted by giving various fertilization treatments by mixing cow urine and EM4 (treatment A); cow urine, EM4, and banana tree trunks (treatment B); cow urine, EM4, and coconut husk (treatment C); and cow urine, EM4, and groundnut root (treatment D). Parameters observed in this study were plant height, number of leaves, stem diameter, and dry biomass weight of the plant. The addition of coconut fiber in cow urine fermentation has a significant effect on plant height parameters. Meanwhile, the addition of groundnut roots to fermented cow urine fertilizer increased the dry biomass weight of the plant significantly. The addition of banana tree trunks to cow urine fermentation significantly affected the stem diameter of curly chili plants. However, the number of leaves did not show a significant difference between all types of fertilization.

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Regeniter, Axel, André Scholer, and Werner H. Siede. "Die quantitative Analyse von Markerproteinen im Urin Quantitative analysis of marker proteins in urine." LaboratoriumsMedizin 29, no. 5 (January 1, 2005): 309–16. http://dx.doi.org/10.1515/jlm.2005.042.

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van Kuilenburg, André B. P., Henk van Lenthe, Monika Löffler, and Albert H. van Gennip. "Analysis of Pyrimidine Synthesis “de Novo” Intermediates in Urine and Dried Urine Filter- Paper Strips with HPLC–Electrospray Tandem Mass Spectrometry." Clinical Chemistry 50, no. 11 (November 1, 2004): 2117–24. http://dx.doi.org/10.1373/clinchem.2004.038869.

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Abstract Background: The concentrations of the pyrimidine “de novo” metabolites and their degradation products in urine are useful indicators for the diagnosis of an inborn error of the pyrimidine de novo pathway or a urea-cycle defect. Until now, no procedure was available that allowed the analysis of all of these metabolites in a single analytical run. We describe a rapid, specific method to measure these metabolites by HPLC–tandem mass spectrometry. Methods: Urine or urine-soaked filter-paper strips were used to measure N-carbamyl-aspartate, dihydroorotate, orotate, orotidine, uridine, and uracil. Reversed-phase HPLC was combined with electrospray ionization tandem mass spectrometry, and detection was performed by multiple-reaction monitoring. Stable-isotope-labeled reference compounds were used as internal standards. Results: All pyrimidine de novo metabolites and their degradation products were measured within a single analytical run of 14 min with lower limits of detection of 0.4–3 μmol/L. The intra- and interassay variation for urine with added compounds was 1.2–5% for urines and 2–9% for filter-paper extracts of the urines. Recoveries of the added metabolites were 97–106% for urine samples and 97–115% for filter-paper extracts of the urines. Analysis of urine samples from patients with a urea-cycle defect or pyrimidine degradation defect showed an aberrant metabolic profile when compared with controls. Conclusion: HPLC with electrospray ionization tandem mass spectrometry allows rapid testing for disorders affecting the pyrimidine de novo pathway. The use of filter-paper strips could facilitate collection, transport, and storage of urine samples.

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Starcher, Barry, and Marti Scott. "Fractionation of Urine to Allow Desmosine Analysis by Radioimmunoassay." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 29, no. 1 (January 1992): 72–78. http://dx.doi.org/10.1177/000456329202900111.

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The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed for desmosine in the RIA. Fractionating the urine prior to hydrolysis with acetone removed one of the major contaminants. The other contaminants could subsequently be removed by extracting the urine hydrolysate with a mixture of chloroform/ethanol (60:40). Samples from nine normal adult urines showed that an average of 45% of the RIA competing material in unfractionated urine was not desmosine. The final extracted residue retained all of the desmosine and only 16% of the original solids. The average adult urine contains approximately 50 pmol desmosine/mg creatinine, reflecting a daily turnover of between 3 and 4 mg of elastin per day.

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Peelen, G. O., J. G. de Jong, and R. A. Wevers. "HPLC analysis of oligosaccharides in urine from oligosaccharidosis patients." Clinical Chemistry 40, no. 6 (June 1, 1994): 914–21. http://dx.doi.org/10.1093/clinchem/40.6.914.

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Abstract Analysis of urinary oligosaccharides by thin-layer chromatography (TLC) is used as screening procedure for 10 different lysosomal diseases. We tested the usefulness of HPLC in screening, using a CarboPac PA1 column (Dionex), pulsed amperometric detection (PAD), and post-column derivatization (PCD). Patterns from six types of oligosaccharidoses were compared with normal urinary patterns and with the TLC patterns. PAD appeared to be nonspecific and therefore is applicable only to desalted urine samples. PCD was more specific and applicable to nondesalted urine samples, albeit with a lower resolving power. Peaks in urines from oligosaccharidoses patients were identified on the basis of retention times of commercially available oligosaccharides or TLC bands after isolation and HPLC of the corresponding oligosaccharides. Abnormal oligosaccharide peaks were seen in urines from patients with alpha-mannosidosis, GM1-gangliosidosis (juvenile), GM2-gangliosidosis (Sandhoff disease), Pompe disease, and beta-mannosidosis. HPLC detected no abnormal oligosaccharides in urine from patients with fucosidosis. Although TLC is a simple and reliable screening procedure for detecting classical lysosomal diseases with oligosaccharide excretion, HPLC, by its higher resolution and possibility of quantification, can more generally be used for recognition of abnormal oligosaccharides or detection of increased excretion or content for known oligosaccharides in urine, other body fluids, and cells.

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Hamid Saad Mohmoud1, Marai. "Dipstick urine analysis screening among asymptomatic dogs of k9 units." Iraqi Journal of Veterinary Medicine 42, no. 1 (2018): 61–64. http://dx.doi.org/10.30539/011.

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WU, Jun, Chao Yan ZHOU, Ming Keong WONG, Hian Kee LEE, Hua CHI, and Choon Nan ONG. "Speciation of Aluminum in Urine." Analytical Sciences 12, no. 4 (1996): 641–45. http://dx.doi.org/10.2116/analsci.12.641.

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Kustyorini, Tri Ida Wahyu, and Permata Ika Hidayati. "Pengaruh perendaman benih pada berbagai jenis larutan urin terhadap daya tumbuh kecambah kaliandra (calliandra calothyrsus)." Jurnal Sains Peternakan 6, no. 01 (June 29, 2018): 47–52. http://dx.doi.org/10.21067/jsp.v6i01.2815.

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Abstrak Penelitian ini bertujuan untuk mengetahui hasil pengaruh perendaman benih pada berbagai jenis larutan urin terhadap daya tumbuh kecambah kaliandra (Calliandra calothyrsus). Materi yang digunakan dalam penelitian ini adalah benih kaliandra sebanyak 100 gr, urin sapi, kambing dan domba sebanyak @1 liter. Metode yang digunakan dalam penelitian ini adalah eksperimental lapang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri atas 5 perlakuan dan 3 ulangan. Perlakuan penelitian terdiri dari P0 (perlakuan kontrol/tanpa perendaman), P1 (perendaman pada air) dan perlakuan eksperimental dengan perendaman pada berbagai jenis urin dengan konsentrasi 10%, yakni, P2 (larutan urin sapi), P3(larutan urin kambing) dan P4 (larutan urin domba). Variabel yang diamati dalam penelitian ini yaitu daya tumbuh kecambah kaliandra yang meliputi persentase perkecambahan, tinggi bibit, persentase benih mati, dan persentase kecambah normal. Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam anova tunggal dengan bantuan aplikasi SPSS for Windows,apabila terdapat pengaruh maka dilanjutkan dengan uji. Perendaman pada urin sapi memberikan nilai terbaik pada persentase kecambah (88,33%), tinggi bibit (5,67±0,57)cm, persentase benih mati terendah (11,67±3,51%), dan persentase kecambah normal (91,67±1,52%). Kesimpulan dari hasil penelitian yaitu perendaman benih pada urin sapi memberikan pengaruh terbaik terhadap daya tumbuh kecambah kaliandara (Calliandra calothyrsus). Abstract This study aims to determine the effect of seed immersion on various types of urine solution on the growth of Calliandra calothyrsus. The material used in this study was 100 grams of calliandra seed, cow urine, goat urine and sheep urine. The method used in this study was a field experiment based on a Completely Randomized Design (CRD) consisting of 5 treatments and 3 replications. The treatment consisted of P0 (control / no soaking treatment), P1 (immersion in water) and experimental treatment with soaking in various types of urine with a concentration of 10%, namely, P2 (cow urine solution), P3 (goat urine solution) and P4 (sheep urine solution). The variables observed in this study were the growth of kaliandra sprouts which included germination percentage, seed height, percentage of dead seeds, and the percentage of normal sprouts. The data obtained were analyzed using a single ANOVA variance analysis with the help of the SPSS for Windows application, if there was an influence then proceed with the test. Immersion in cow urine gave the best value in the percentage of sprouts (88.33%), seedling height (5.67 ± 0.57) cm, the lowest percentage of dead seeds (11.67 ± 3.51%), and the percentage of normal sprouts ( 91.67 ± 1.52%). The conclusion of the research results is that the immersion of seeds in cow urine gives the best effect on the power of kaliandara sprouts (Calliandra calothyrsus).

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Nowrousian, M. R., D. Brandhorst, C. Sammet, M. Kellert, R. Daniels, P. Schuett, M. Poser, et al. "Relationship between Serum Concentrations and Urinary Excretions of Monoclonal Free Light Chains (mFLC) Detectable as Bence Jones Proteins (BJP) by Immunofixation Electrophoresis (IFE) in Patients with Multiple Myeloma (MM)." Blood 106, no. 11 (November 16, 2005): 5060. http://dx.doi.org/10.1182/blood.v106.11.5060.5060.

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Abstract Introduction. mFLC are important markers for the diagnosis and monitoring of MM. This study for the first time determines serum concentrations of mFLC which are required to produce renal overflow and BJP in urine detectable by IFE and evaluates the relationship between urinary excretions of mFLC and renal function. Patients and methods. 378 paired samples of serum and 24-h-urine from 82 patients were evaluated during the course of their disease. Serum FLC concentrations were measured nephelometrically using an automated immunoassay. Urine samples were tested for clonal bands using agarose gel electrophoresis, scanning densitometry and visual checking of electrophoretic gels. BJP were identified by urine IFE. Results. Among the 378 serum samples, 173 (46%) were normal and 205 (54%) were abnormal for FLC k/l ratios, indicating the presence of mFLC, 98 of kappa and 107 of lambda type. In 98 serum samples with mFLC kappa, 48 (49%) were associated with negative urine IFE analysis and 50 (51%) had positive urine tests. The median serum kappa concentrations were 40 mg/L (range 6–710) for negative urines and 113 mg/L (range 7–39500) for positive urines (p=0.001), indicating an almost threefold greater median value which was approximately six times the upper limit of the reference range (3.3.–19.4 mg/L) for samples with positive urine IFE analysis. In 107 serum samples with mFLC lambda, 70 (65%) were negative in urine and 37 (35%) were positive. The median serum concentrations associated with negative urine IFE tests were 44 mg/L (range 3–561) and were 278 mg/L (range 5–7060) for positive urines (p=0.0001), indicating an almost sixfold difference. This was approximately 2.5-fold greater than for kappa, and approximately 11 times the upper limit of the reference range (5.7–26.3 mg/L) for samples with positive urine IFE analysis. Renal excretions of mFLC, in addition, were determined primarily by serum concentrations for lambda, but by serum concentrations, renal function and, probably, molecular changes for kappa. For both, renal excretions significantly decreased at high serum concentrations combined with renal dysfunction. Conclusion. Based on these results, relatively high serum concentrations of mFLC are required to produce renal overflow and positive urine IFE tests for BJP. Furthermore, urine excretions of mFLC are determined primarily by serum concentrations, but also by renal function, particularly for kappa.

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Degen, Gisela H., Jörg Reinders, Martin Kraft, Wolfgang Völkel, Felicia Gerull, Rafael Burghardt, Silvia Sievering, et al. "Citrinin Exposure in Germany: Urine Biomarker Analysis in Children and Adults." Toxins 15, no. 1 (December 30, 2022): 26. http://dx.doi.org/10.3390/toxins15010026.

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Citrinin (CIT), a mycotoxin known to exert nephrotoxicity, is a contaminant in food and feed. Since CIT contamination is not regularly analyzed, data on its occurrence and especially levels in food commodities are insufficient for conducting a conventional exposure assessment. Yet, human biomonitoring, i.e., an analysis of CIT and its metabolite dihydrocitrinone (DH-CIT) in urine samples allows to estimate exposure. This study investigated CIT exposure in young (2–14 years) and adult (24–61 years) residents of three federal states in Germany. A total of 179 urine samples from children and 142 from adults were collected and analyzed by a targeted LC-MS/MS based method for presence of CIT and DH-CIT. At least one of the biomarkers was detected and quantified in all urines, which indicated a widespread dietary exposure to the mycotoxin in Germany. Interestingly, the biomarker concentrations of CITtotal (sum of CIT and DH-CIT) were higher in children’s urine (range 0.05–7.62 ng/mL; median of 0.54 ng/mL) than in urines from adults (range 0.04–3.5 ng/mL; median 0.3 ng/mL). The biomarker levels (CITtotal) of individual urines served to calculate the probable daily CIT intake, for comparison to a value of 0.2 µg/kg bw/day defined as ‘level of no concern for nephrotoxicity’ by the European Food Safety Authority. The median exposure of German adults was 0.013 µg/kg b.w., with only one urine donor exceeding this provisional tolerable daily intake (pTDI) for CIT. The median exposure of children was 0.05 µg/kg bw per day (i.e., 25% of the pTDI); however, CIT exposure in 12 individuals (6.3% of our study group) exceeded the limit value, with a maximum intake of 0.46 µg/kg b.w. per day. In conclusion, these results show evidence for non-negligible exposure to CIT in some individuals in Germany, mainly in children. Therefore, further biomonitoring studies and investigations aimed to identify the major sources of CIT exposure in food commodities are required.

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Febryansah, M. Iqbal, Anton Yudhana, and Alfian Ma'arif. "Urinoir Analyzer Pintar Pendeteksi Kelainan Pada Fungsi Ginjal Dengan Analisis Kadar Ph Dan Warna Pada Urin." Mobile and Forensics 2, no. 1 (March 31, 2020): 32–40. http://dx.doi.org/10.12928/mf.v2i1.2032.

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Perkembangan pemeriksaan penyakit kelainan ginjal melalui analisa urin saat ini dilakukan dalam dua proses pemeriksaan secara makroskopis dan mikroskopis. Pada dasarnya dibutuhkan sebuah alat yang mampu memproses dan menganalisis sebuah sampel urin secara otomatis agar tidak terjadinya kesalahan dalam melakukan pemeriksaan penyakit melalui sampel urin. Awalan perkembangan ini menggunakan sebuah Kontroler Arduino UNO dan dua buah variabel masukan yaitu sensor warna TCS3200 dan sensor PH meter SKU SEN0161. Dua buah variabel masukan sensor bekerja secara berdampingan dengan sensor TCS3200 memiliki hasil keluaran berupa nilai frekuensi RGB dan diproses kembali menjadi frekuensi keabuan. Lalu, sensor PH meter SKU SEN0161 menghasilkan sebuah nilai PH pada sampel urin. Hasil dari pemeriksaan tersebut ditampilkan pada sebuah penampil berupa LCD berukuran 16x2. Hasil pemeriksaan dari alat ini dibandingkan dengan hasil analisa pakar dari Balai Laboratorium Kesehatan Yogyakarta bagian Urology. dan mendapatkan tingkatan nilai akurasi 93% dengan keberhasilan data sebanyak 28 dari 30 data yang diambil.Â The development of examining kidney disorders through urine analysis is currently carried out in two processes of examination, macroscopic and microscopic. Basically, we need a tool that is able to process and analyze a urine sample automatically so that there are no errors in carrying out disease checks through the urine sample. The beginning of this development used an Arduino UNO controller and two input variables, namely the TCS3200 color sensor and the SKU SEN0161 PH meter sensor. Two sensor input variables working side by side with the TCS3200 sensor have an output in the form of RGB frequency values and are processed back into gray frequencies. Then, the PH meter SKU SEN0161 sensor generates a PH value in the urine sample. The results of these checks are displayed on a 16x2 LCD display. The examination results of this tool are compared with the results of the analysis of experts from the Yogyakarta Health Laboratory Center, Urology section. and get an accuracy level of 93% with the success of the data as much as 28 of the 30 data taken.

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Harnly, James, Craig Charron, David Baer, and Janet Novotny. "Elimination of the Variance Between Individuals Is Necessary to Evaluate the Impact of Garlic on the Metabolic Profile of Human Urine." Current Developments in Nutrition 4, Supplement_2 (May 29, 2020): 402. http://dx.doi.org/10.1093/cdn/nzaa045_035.

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Abstract Objectives To determine the impact of garlic on the metabolic profile of urine. Methods On the first day 17 fasting subjects were fed a breakfast of bread and butter. Urines were collected before and 3 hours after the meal. On a second day, the same 17 fasting subjects were fed a meal of bread, butter, and garlic. Urines were again collected before and 3 hours after the meal. Samples were analyzed by metabolomics using liquid chromatography-mass spectrometry (LC-MS) and data were subjected to analysis of variance-principal component analysis (ANOVA-PCA). Results 637 compounds were found in the urines and 277 were identified. PCA of urine profiles were dominated by variation between individual. Removal of individual variance by ANOVA allowed differentiation of fasting urines from bread and butter urines from bread, butter, and garlic urines. PCA loadings identified compounds that led to discrimination between treatments. Influence of the loading identified compounds were verified by examination of the LC-MS data for individual compounds. Three unique sulfur containing compounds were identified. Loadings showed, however, that a change in the metabolite profiles (ratios of compounds) and not the unique compounds) were most informative. Conclusions Removal of variance between individuals is essential to properly analyze the data. Changes in the patterns of compounds routinely observed in urine were the major result of the garlic meal. ANOVA-PCA is an excellent tool for isolating experimental factors. Funding Sources Agricultural Research Service, US Department of Agriculture and Office of Dietary Supplements, National Institutes of Health.

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Naik, Dr Preeta. "A Study of Dipstick and Microscopic Analysis of Formed Elements in Urine." Journal of Medical Science And clinical Research 05, no. 04 (April 18, 2017): 20485–88. http://dx.doi.org/10.18535/jmscr/v5i4.122.

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Young, David C., Sandra Craft, Mary-Clare Day, Barbara Davis, Elizabeth Hartwell, and Song Tong. "Comparison of Abbott LCxChlamydia trachomatisAssay With Gen-Probe PACE2 and Culture." Infectious Diseases in Obstetrics and Gynecology 8, no. 2 (2000): 112–15. http://dx.doi.org/10.1155/s1064744900000119.

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In this study the LCx assay (a nucleic acid amplification assay) forChlamydia trachomatisin endocervical samples was compared with the Gen-Probe PACE2 assay (a nucleic acid probe assay) for endocervical samples, and with endocervical culture. In addition, the efficacy of the LCx assay was determined for midstream clean-catch urine samples because it is often necessary to obtain such a sample for routine urine culture and it is simpler to collect only a single sample without also collecting a first-void urine for LCx. Endocervical specimens from 205 patients were tested forC. trachomatisvia LCx and PACE2. Of these patients, 203 were tested by culture. Midstream cleancatch urine samples from 75 of these patients were tested by LCx. The sensitivities and specificities for these assays, after discrepant analysis, were 100 and 98.9% for LCx of endocervical samples, 52.4 and 100% for PACE2; and 71.4 and 100% for culture. The sensitivity/specificity of LCx for midstream clean-catch urines was 66.7/98.5%. The apparent prevalence ofC. trachomatisin our population was 10.2%. These data indicate that among the methods tested, LCx of endocervical samples had the highest sensitivity forC. trachomatisin this population. The senstivity of the urine LCx assay using midstream clean-catch collected urines was considerably less than that reported in other studies that used first-void urines but was higher than that of PACE2. Infect. Dis. Obstet. Gynecol. 8:112–115, 2000.

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Blijdorp, Charles J., Omar A. Z. Tutakhel, Thomas A. Hartjes, Thierry P. P. van den Bosch, Martijn H. van Heugten, Juan Pablo Rigalli, Rob Willemsen, et al. "Comparing Approaches to Normalize, Quantify, and Characterize Urinary Extracellular Vesicles." Journal of the American Society of Nephrology 32, no. 5 (March 29, 2021): 1210–26. http://dx.doi.org/10.1681/asn.2020081142.

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BackgroundUrinary extracellular vesicles (uEVs) are a promising source for biomarker discovery, but optimal approaches for normalization, quantification, and characterization in spot urines are unclear.MethodsUrine samples were analyzed in a water-loading study, from healthy subjects and patients with kidney disease. Urine particles were quantified in whole urine using nanoparticle tracking analysis (NTA), time-resolved fluorescence immunoassay (TR-FIA), and EVQuant, a novel method quantifying particles via gel immobilization.ResultsUrine particle and creatinine concentrations were highly correlated in the water-loading study (R2 0.96) and in random spot urines from healthy subjects (R2 0.47–0.95) and patients (R2 0.41–0.81). Water loading reduced aquaporin-2 but increased Tamm-Horsfall protein (THP) and particle detection by NTA. This finding was attributed to hypotonicity increasing uEV size (more EVs reach the NTA size detection limit) and reducing THP polymerization. Adding THP to urine also significantly increased particle count by NTA. In both fluorescence NTA and EVQuant, adding 0.01% SDS maintained uEV integrity and increased aquaporin-2 detection. Comparison of intracellular- and extracellular-epitope antibodies suggested the presence of reverse topology uEVs. The exosome markers CD9 and CD63 colocalized and immunoprecipitated selectively with distal nephron markers.Conclusions uEV concentration is highly correlated with urine creatinine, potentially replacing the need for uEV quantification to normalize spot urines. Additional findings relevant for future uEV studies in whole urine include the interference of THP with NTA, excretion of larger uEVs in dilute urine, the ability to use detergent to increase intracellular-epitope recognition in uEVs, and CD9 or CD63 capture of nephron segment–specific EVs.

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Al Ayoubi, Manar, Mohammad Salman, Lucia Gambacorta, Nada El Darra, and Michele Solfrizzo. "Assessment of Dietary Exposure to Ochratoxin A in Lebanese Students and Its Urinary Biomarker Analysis." Toxins 13, no. 11 (November 10, 2021): 795. http://dx.doi.org/10.3390/toxins13110795.

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The present study investigated the dietary and urinary OTA occurrence among 44 Lebanese children. Relying on HPLC-FLD analysis, OTA was found in all the urine samples and in 46.5% and 25% of the 24 h duplicate diet and dinner samples, respectively. The means of OTA levels in positive samples were 0.32 ± 0.1 ng/g in 24 h diet, 0.32 ± 0.18 ng/g in dinner and 0.022 ± 0.012 ng/mL in urines. These values corresponded to margin of exposure (MOE) means of 7907 ± 5922 (neoplastic) and 2579 ± 1932 (non-neoplastic) calculated from positive 24 h diet, while 961 ± 599 (neoplastic) and 313 ± 195 (non-neoplastic) calculated from the urine. Since the MOE levels for the neoplastic effect were below the limit (10,000), a major health threat was detected and must be addressed as a health institutions’ priority. Besides, the wide difference between PDIs and MOEs calculated from food and urine suggests conducting further OTA’s toxicokinetics studies before using urine to measure OTA exposure.

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SERA, K., Y. MIURA, and S. FUTATSUGAWA. "APPLICATION OF A STANDARD-FREE METHOD TO QUANTITATIVE ANALYSIS OF URINE SAMPLES." International Journal of PIXE 11, no. 03n04 (January 2001): 149–58. http://dx.doi.org/10.1142/s0129083501000207.

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A standard-free method of quantitative analysis, which is based on the fact that the total yield of continuous x-rays from the sample approximately corresponds to effective weight of the sample, was developed and has been applied to some typical bio-samples such as serum, whole blood, hair and untreated bone. In this work, the standard-free method was applied to untreated urine samples. This method allows us to perform sample preparation only by dropping 5 μl of urine sample onto a backing film. It requires neither a large amount of urine nor the internal standard. As the results, values of concentration of potassium for 4 samples agree well with the value obtained by the internal standard method within an error of 10%. The present method was also applied to 21 urine samples containing excess amount of urinary protein and / or sugar, and it is found that the present method is applicable to such abnormal urines. Owing to this method, target preparation can be performed at the place and time of sampling. It is quite convenient to environmental studies.

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Sánchez-Carbayo, Marta, Antonia Espasa, Virtudes Chinchilla, Enrique Herrero, Julián Megías, Antonio Mira, and Federico Soria. "New Electrochemiluminescent Immunoassay for the Determination of CYFRA 21-1: Analytical Evaluation and Clinical Diagnostic Performance in Urine Samples of Patients with Bladder Cancer." Clinical Chemistry 45, no. 11 (November 1, 1999): 1944–53. http://dx.doi.org/10.1093/clinchem/45.11.1944.

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Abstract Background: A new electrochemiluminescent immunoassay (ECLIA) has been developed for the determination of cytokeratin 19 (CYFRA 21-1) in the Elecsys 2010 immunoassay system. Urinary CYFRA 21-1 might have a role in the diagnosis of bladder cancer. Methods: We performed an analytical evaluation of the CYFRA 21-1 ECLIA for serum and urine samples. The clinical value of urinary CYFRA 21-1 for the detection of bladder cancer was evaluated through its measurement in 226 urine samples from symptomatic and asymptomatic controls. Results: At concentrations of 2–30 μg/L, within-assay imprecision (CV) was below 2.1% for sera and 3.3% for urines, with interassay CVs below 3.3% for sera and 4.9% for urines. The day-to-day CV was <20% at concentrations >0.2 μg/L (functional sensitivity). Measurement of diluted samples showed that the assay estimated CYFRA 21-1 between 98% and 103% for sera and 98% and 105% for urines. Recovery of added CYFRA 21-1 was 99–105% for sera and 96–115% for urines. We separately compared serum and urine CYFRA 21-1 ECLIA results with those obtained with an IRMA (CIS bio international). Regression analysis for sera was: CYFRA 21-1 (ECLIA) = 0.520 + 1.018 CYFRA 21-1 (IRMA); [95% confidence interval (CI) (y-intercept), −0.260 to 1.309]; 95% CI (slope), 0.978–1.060; n = 100; Sy|x = 3.242; r2 = 0.987. For urine samples it was: CYFRA 21-1 (ECLIA) = 0.716 + 0.966 CYFRA 21-1 (IRMA); 95% CI (y-intercept), 0.009–1.422; 95% CI (slope), 0.956–0.976; n = 100; Sy|x = 4.136; r2 = 0.986. In urine samples voided by patients with and without bladder cancer, the best ROC analysis discrimination provided 81.0% (95% CI, 72.7–87.7%) sensitivity and 97.2% (95% CI, 90.2–99.6%) specificity at a threshold value of 5.7 μg/L. Conclusions: Our initial evaluation showed reliable analytical performance for urinary CYFRA 21-1, which might assist urologists in the detection of bladder cancer as a noninvasive adjunct to cystoscopy.

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Gaspari, Valeria, Camilla Ceccarani, Marco Severgnini, Gionathan Orioni, Tania Camboni, Luca Laghi, Sara Morselli, et al. "First-Void Urine Microbiome in Women with Chlamydia trachomatis Infection." International Journal of Molecular Sciences 23, no. 10 (May 17, 2022): 5625. http://dx.doi.org/10.3390/ijms23105625.

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Background: Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized the microbiome and metabolome profiles of first-void urines in a cohort of women with CT urethral infection attending an STI clinic. Methods: Based on CT positivity by nucleic acid amplification techniques on urine samples, the enrolled women were divided into two groups, i.e., “CT-negative” (n = 21) and “CT-positive” (n = 11). Urine samples were employed for (i) the microbiome profile analysis by means of 16s rRNA gene sequencing and (ii) the metabolome analysis by 1H-NMR. Results: Irrespective of CT infection, the microbiome of first-void urines was mainly dominated by Lactobacillus, L. iners and L. crispatus being the most represented species. CT-positive samples were characterized by reduced microbial biodiversity compared to the controls. Moreover, a significant reduction of the Mycoplasmataceae family—in particular, of the Ureaplasma parvum species—was observed during CT infection. The Chlamydia genus was positively correlated with urine hippurate and lactulose. Conclusions: These data can help elucidate the pathogenesis of chlamydial urogenital infections, as well as to set up innovative diagnostic and therapeutic approaches.

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Zhao, Hongda, Ryan Tsz-Hei Tse, Carol Ka-Lo Cheng, Christine Yim-Ping Wong, Angel Wing-Yan Kong, Ronald Cheong-Kin Chan, Peter Ka-Fung Chiu, Chi-Fai Ng, and Jeremy Yuen-Chun Teoh. "In vitro cytotoxicity of human urine and its potential toxic parameters towards bladder cancer cells." PLOS ONE 17, no. 10 (October 19, 2022): e0276127. http://dx.doi.org/10.1371/journal.pone.0276127.

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Background Bladder cancer (CaB) has a high recurrence rate despite surgery. As bladder is constantly filled with urine, it is worthwhile to investigate whether it could have any detrimental effects on bladder cancer cells. Methods We investigated the cytotoxicity of urine samples from CaB patients and normal controls on four CaB cell lines and tested the percentage of cell death, proliferation, adhesion, invasion and colonies formation ability. In order to identify the potential components involving in urine cytotoxicity, we evaluated some basic physiochemical parameters of urines, such as pH, osmolarity, creatinine (Cr), sodium (Na), potassium (K), chloride (Cl), calcium (Ca) and phosphate (PO4). We further compared the pH values of urine samples between CaB who developed recurrence versus those who did not. A more in-depth analysis on inflammatory markers was performed for two representative urine samples which demonstrated opposite cytoxic effects. Results 23 CaB patients and 20 normal controls were recruited into this study. According to in vitro experiments, both CaB and non-CaB urines had comparable effect on cell toxicity, proliferation, adhesion, invasion and colonies formation ability in four cell lines, HTB9, RT4, T24 and UMUC3, while RT4 was the most sensitive to urine toxicity. After evaluating the relationship between basic physiochemical parameters and cytotoxicity, we found out that there were strong negative correlations between pH value and 24 hours death rate for the 4 CaB cell lines (HTB9 r = -0.6651, p<0.001; RT4 r = -0.8335, p<0.001; T24 r = -0.4924, p<0.001; UMUC3 r = -0.7066, p<0.001). Osmolarity, urine Cr and PO4 all had weakly or moderately positive correlations with CaB cells on 24 hours death rate. CaB patients who developed recurrence had more alkaline urine than those who did not develop recurrence. In the urine sample with the highest cytoxicity, high concentrations of IL-6 and IFN-gamma were found. Conclusions Our study confirmed that there was not statistically significant difference in cytotoxicity between CaB and non-CaB urines. However, we identified some parameters that could have an impact on cytotoxicity towards CaB cells. Modifying certain urine characteristics peri-operatively may induce cytotoxicity, avoid tumour re-implantation, and reduce the chance of cancer recurrence.

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SUZUKI, Yuji. "Spectrophotometric Determination of Urine Bilirubin by p-Dimethylaminobenzaldehyde." Analytical Sciences 14, no. 3 (1998): 609–12. http://dx.doi.org/10.2116/analsci.14.609.

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TOIDA, Toshihiko, Naoto KAKINUMA, Hidenao TOYODA, and Toshio IMANARI. "1H-NMR Profile of Glycosaminoglycans in Human Urine." Analytical Sciences 10, no. 4 (1994): 537–41. http://dx.doi.org/10.2116/analsci.10.537.

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Bargotya, Mona, Lalit Kumar, Pinkey Kachhap, Payel Das, Vidushi Sachdeva, and Sonali Bhattar. "A Flow Cytometric and Cytochemistric Analysis of Urine to Detect Early Urinary Tract Infection." Annals of Pathology and Laboratory Medicine 7, no. 1 (January 25, 2020): A7–12. http://dx.doi.org/10.21276/apalm.2659.

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Saputra, Haryanto, Harmadi Harmadi, and Afdhal Muttaqin. "Analisis Pengaruh Macrobending Pada Sensor Albumin Urin Dengan Metode Evanescent Menggunakan Serat Optik FD 620-10." JURNAL ILMU FISIKA | UNIVERSITAS ANDALAS 11, no. 1 (July 15, 2019): 31–36. http://dx.doi.org/10.25077/jif.11.1.31-36.2019.

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Telah dilakukan analisis pengaruh macrobending pada sensor albumin urin dengan metode evanescent menggunakan serat optik FD 620-10. Sumber cahaya dari laser dioda dengan panjang gelombang 650 nm ditransmisikan pada serat optik multimode. Pengupasan cladding serat optik sepanjang 3 cm mengakibatkan pelemahan intensitas terukur pada detektor akibat interaksi gelombang evanescent dengan molekul albumin dalam urine. Sensor albumin urin dikarakterisasi berdasarkan tegangan keluaran fotodioda. Hasil karakterisasi sensor menunjukkan serat optik pada jari-jari bending 2,5 cm adalah yang optimal untuk pengukuran kadar albumin urin dengan sensitivitas sensor sebesar 0.001 (mg/dL)/V. Tegangan keluaran fotodioda semakin mengecil seiring meningkatnya kadar albumin urin.Kata kunci : macrobending, serat optik, albumin urin.

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Wagener, R. E., M. W. Linder, and R. Valdes. "Decreased signal in Emit assays of drugs of abuse in urine after ingestion of aspirin: potential for false-negative results." Clinical Chemistry 40, no. 4 (April 1, 1994): 608–12. http://dx.doi.org/10.1093/clinchem/40.4.608.

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Abstract During routine drug analysis with the Syva d.a.u. Emit immunoassays we observed a high frequency of urines with lower rates of changes in absorbance (delta A R) than the rate for a drug-free urine calibrator. Many of these urines contained salicylates. Among 40 urines with apparent salicylate concentrations between 15 and 420 mg/dL tested for benzoylecgonine (BE), 20 had delta A R < -4 (range +2 to -28 mA/min). The rates decreased with increasing salicylate: delta A R = -0.057 x (salicylate, mg/dL) -0.22 mA/min (r = 0.85, n = 40, P < 0.01). Urines from 100 control subjects (no salicylate) had mean +/- SD delta A R values of -1.05 +/- 2.2 mA/min (range +3 to -7; only two were < -4 mA/min). Although direct addition of salicylic acid (200 mg/dL) to urine specimens did not reproduce the negative bias, ingestion of aspirin (acetylsalicylic acid) did by -0.09 mA/min per 1 mg/dL (72.4 mumol/L) salicylate. Negative biases observed for other Emit d.a.u. assays after salicylate ingestion lead us to conclude that ingestion of therapeutic doses of aspirin may cause false-negative results for drug screens in urines by this technology.

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Sreedharan, Shilpa, John A. Petros, Viraj A. Master, Kenneth Ogan, John G. Pattaras, David L. Roberts, Fei Lian, and Rebecca S. Arnold. "Aquaporin-1 Protein Levels Elevated in Fresh Urine of Renal Cell Carcinoma Patients: Potential Use for Screening and Classification of Incidental Renal Lesions." Disease Markers 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/135649.

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Introduction and Objectives. There are over 65,000 new cases of renal cell carcinoma (RCC) each year, yet there is no effective clinical screening test for RCC. A single report claimed no overlap between urine levels of aquaporin-1 (AQP1) in patients with and without RCC (Mayo Clin Proc. 85:413, 2010). Here, we used archived and fresh RCC patient urine to validate this report.Methods. Archived RCC, fresh prenephrectomy RCC, and non-RCC negative control urines were processed for Western blot analysis. Urinary creatinine concentrations were quantified by the Jaffe reaction (Nephron 16:31, 1976). Precipitated protein was dissolved in 1x SDS for a final concentration of 2 μg/µL creatinine.Results. Negative control and archived RCC patient urine failed to show any AQP1 protein by Western blot analysis. Fresh RCC patient urine is robustly positive for AQP1. There was no signal overlap between fresh RCC and negative control, making differentiation straightforward.Conclusions. Our data confirms that fresh urine of patients with RCC contains easily detectable AQP1 protein. However, archival specimens showed an absence of detectable AQP1 indistinguishable from negative control. These findings suggest that a clinically applicable diagnostic test for AQP1 in fresh urine may be useful for detecting RCC.

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Iman Ridwan, Sri Anggraeni, and Bambang Supriyatno. "Analisis Lembar Kerja Peserta Didik Sekolah Menengah Atas Pada Praktikum Uji Urin." BIODIK 6, no. 3 (September 6, 2020): 321–31. http://dx.doi.org/10.22437/bio.v6i3.9462.

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The student’s worksheet is particularly helpful for students in practical activities to stimulate a whole concept formation that previously had been obtained from either the literature or the delivery of the teacher in the class. This study aims to explain the analyses and trials of several imputations that refer to KTSP curriculum and the 2013 curriculum. The research method used is a quantitative description. The research method used in this study is a quantitative descriptive method. The sample in this study amounts to five student worksheets of the urine tested chosen using an alcoholological sample. The research tools used in this study are the features of conceptual analysis, durations, and knowledge construction, the completeness of the components of the lto-to, and the finder of lt-based vee diagrams adapted from novak and gowin (1984). Research shows that the conceptually student worksheet urine test has not yet contained content and trained competence (knowledge and skills) in accordance with the demands of the 2013 curriculum. Procedurally, the analyzed student’s worksheet of urine tests may be well performed, but it is largely irrelevant to the practical purposes and basic competence demands in the curriculum. From the standpoint of the construction of knowledge, the analysis of student’s worksheet urine test has not helped learners to reconstruct their knowledge in its entirety of concepts, principles, and theories through the various facts that result. Judging from its structure, almost all of student’s worksheet tested of urine tests has the components of a complete vee diagram, though with the difference in quality indicated by the score score of each of the components of the vee diagram. Based on the problems found, the lentine requires reconstruction from the conceptual side, procedural, construction of knowledge, as well as its structure. Abstrak. Lembar Kerja Peserta Didik (LKPD) sangat bermanfaat bagi siswa dalam kegiatan praktikum untuk menstimulasi pembentukan konsep secara utuh yang sebelumnya sudah didapatkan baik dari studi literatur atau penyampaian dari guru di dalam kelas. Penelitian ini bertujuan untuk menjelaskan analisis serta uji coba dari beberapa LKPD praktikum yang mengacu pada kurikulum KTSP dan kurikulum 2013. Metode penelitian yang digunakan adalah deskriptif kuantitatif. Metode penelitian yang digunakan dalam penelitian ini adalah metode deskriptif kuantitatif. Sampel dalam penelitian ini berjumlah 5 LKPD Uji Urin yang dipilih dengan menggunakan teknik purposive sampling. Instrumen penelitian yang digunakan dalam penelitian ini adalah rubrik analisis konseptual, prosedural, dan konstruksi pengetahuan, rubrik kelengkapan komponen LKPD, dan rubrik penskoran komponen LKPD berdasarkan Diagram Vee yang diadaptasi dari Novak dan Gowin (1984). Hasil penelitian menunjukkan bahwa secara konseptual LKPD Uji Urin yang dianalisis belum memuat konten dan melatih kompetensi (pengetahuan dan keterampilan) yang sesuai dengan tuntutan Kurikulum 2013. Secara prosedural, LKPD Uji Urin yang dianalisis dapat dilaksanakan dengan baik, tetapi sebagian besar belum relevan dengan tujuan praktikum dan tuntutan kompetensi dasar di kurikulum. Dari segi konstruksi pengetahuan, LKPD Uji Urin yang dianalisis belum membantu peserta didik untuk mengonstruksi pengetahuannya secara utuh yang terdiri dari konsep, prinsip, dan teori melalui berbagai fakta yang dihasilkan. Ditinjau dari strukturnya, hampir seluruh LKPD Uji Urin yang dianalisis memiliki komponen Diagram Vee lengkap, meskipun dengan perbedaan kualitas yang ditunjukkan oleh capaian skor dari masing-masing komponen Diagram Vee. Berdasarkan permasalahan-permasalahan yang ditemukan, LKPD Uji Urin memerlukan rekonstruksi dari sisi konseptual, prosedural, konstruksi pengetahuan, maupun strukturnya.

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Perkins, S. L., and P. M. Johnson. "Loss of porphyrins from solution during analysis: effect of sample pH and matrix on porphyrin quantification in urine by "high-performance" liquid chromatography." Clinical Chemistry 35, no. 7 (July 1, 1989): 1508–12. http://dx.doi.org/10.1093/clinchem/35.7.1508.

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Abstract We report the effect of sample matrix and pH on quantification of porphyrins by HPLC with fluorimetric detection. For aqueous solutions of pH less than 2.5, HPLC peak heights of the porphyrins increased with decreasing pH, reaching a plateau at pH less than 1.0. This loss of porphyrins from solutions with pH greater than 1.0 appeared to be due to a combination of microprecipitation and aggregation effects. No such "pH effect" was observed for urine samples supplemented with mixed-porphyrin standards. Addition of trace amounts of albumin to aqueous solutions also decreased these pH-related losses. These findings suggest a porphyrin-protein interaction that prevents microprecipitation and aggregation processes. We conclude that standard solutions of porphyrins for HPLC analysis should be prepared in a urine matrix. If aqueous solutions are used, then the pH must be adjusted to less than 1.0. Urine samples from normal individuals require only adjustment of pH to less than 2 before analysis; however, porphyric urines requiring dilution should be prepared with porphyrin-free urine diluent.

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FUSHINUKI, Yoshito, and Isao TANIGUCHI. "Determination of Methylamphetamine in Urine by Differential Pulse Polarography." Analytical Sciences 14, no. 2 (1998): 265–68. http://dx.doi.org/10.2116/analsci.14.265.

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M. SIMONET, Bartolomé, Félix GRASES, and Juan G. MARCH. "Enzymatic Determination of Pyrophosphate in Urine by Flow Methods." Analytical Sciences 19, no. 7 (2003): 1029–32. http://dx.doi.org/10.2116/analsci.19.1029.

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Septiani, Novi Dwi, Hariyono Hariyono, and Inayatur Rosyidah. "Hubungan Pesalinan Kala II Lama dengan Kejadian Retensio Urine." Jurnal Kebidanan 11, no. 1 (June 10, 2021): 1–10. http://dx.doi.org/10.35874/jib.v11i1.849.

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Persalinan kala 2 lama menimbulkan efek berbahaya baik terhadap ibu maupun janin. Retensio urine adalah suatu gangguan buang air kecil, dimana terjadi lemahnya pancaran urine, tidak lancar serta rasa ada yang tersisa dan tidak puas, dapat disertai keinginan untuk mengedan atau memberikan tekanan pada suprapubik saat buang air kecil. Tujuan Penelitian ini adalah mengidentifikasi hubungan antara persalinan kala 2 lama dengan kejadian retensio urine di Puskesmas Baureno Bojonegoro. Penelitian ini merupakan jenis penelitian survey analitik retrospektif, rancanganberupa case control dengan pendekatan retrospektif. Populasi dan sampling dalam penelitian ini yaitu total sampling persalinan kala 2 lama dan kasus retensio urine berdasarkan diagnose medis pasien di poned Puskesmas Baureno dari bulan November 2019 sampai April 2020 yaitu 34 orang. Variabel independen persalinan kala 2 lama dan Variabel dependen Kejadian retensio urine, Teknik Analisa menggunakan Analisa univariat dan Analisa bivariat menggunakan Uji Rank Spearman. Hasil dari penelitian menunjukkan bahwa seluruh responden 34 (100,0%) merupakan Persalinan Kala 2 Lama dengan sebanyak 21 responden (62.8%) mengalami Retensio urin, pada Analisa uji spearman rankdidapatkan p value= 0,038 dimana p value < 0,05 maka H1 diterima yang artinya ada hubungan Persalinan kala II Lama dengan kejadian Reteniso Urine Pada Ibu Bersalin Puskesmas Baureno Kabupaten Bojonegoro. Penelitian ini dapat dianalisis, Persalinan kala II Lama berhubungan dengan kejadian Reteniso Urine

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Ragone, Angela, Alessia Salzillo, Annamaria Spina, Silvia Zappavigna, Michele Caraglia, Luigi Sapio, and Silvio Naviglio. "Protein Kinase A Detection in Human Urine Samples." Journal of Clinical Medicine 10, no. 18 (September 10, 2021): 4096. http://dx.doi.org/10.3390/jcm10184096.

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Actively involved in tumor maintenance, cAMP-dependent protein kinase A (PKA) has been proposed as a putative biomarker in cancer. Recently, an active PKA form has been identified in human sera and PKA autoantibodies have been detected in cancer patients. However, their serum functions, as well as diagnostic significance, remain largely unknown. Although several PKA detection assays have been developed, none refer to a laboratory diagnostic procedure. Among these, ELISA and Western blotting (WB) assays have been employed in PKA detection. Since, to the best of our knowledge, there are no data showing its presence in human urine samples, herein, we explore the possibility of PKA’s existence in this biological specimen. Interestingly, among the 30 screened urines by quantitative sandwich ELISA, we recognized detectable PKA levels in 5 different samples, and of those two exhibited a considerable high concentration. To corroborate these results, we also evaluated PKA’s presence in both positive and negative ELISA urines by WB. Remarkably, immunoblotting analysis confirmed PKA’s existence in certain, but not in all, human urine specimens. Despite being quite preliminary, these findings firstly identify PKA in urine samples and provide evidence for its potential clinic usage as a diagnostic analyte in laboratory medicine.

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Pourbakhshi, Yasaman, Abdul Rahman Bahramy, Farshid Ghorbani Shanha, Mohammad Javad Assari, Leila Tajik, and Maryam Farhadian. "Development of Cold Fiber Head Space Solid-Phase Microextraction for Analysis of 2,5 Hexandion in Urine." Chemistry & Chemical Technology 13, no. 4 (December 15, 2019): 482–88. http://dx.doi.org/10.23939/chcht13.04.482.

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Colston, K., N. J. Williams, and H. J. Cleeve. "Studies on vitamin D binding protein in the nephrotic syndrome." Clinical Chemistry 31, no. 5 (May 1, 1985): 718–21. http://dx.doi.org/10.1093/clinchem/31.5.718.

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Abstract We studied the properties of vitamin D binding protein in plasma and urine from nine patients with nephrotic syndrome. Samples were incubated with 25-[3H]hydroxyvitamin D3, after which we determined binding capacity and apparent dissociation constants. Binding capacity was markedly less in plasma from patients with nephrotic syndrome than that from normal subjects, but binding affinity was unchanged. Specific binding of 25-hydroxy[3H]vitamin D3 could be demonstrated in urine from all the nephrotic patients, and sucrose density-gradient analysis of these urines revealed a single binding peak with sedimentation characteristics similar to those of vitamin D binding protein in plasma.

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Jackson, P. J., C. J. Sampson, E. H. Cooper, D. Heney, and J. T. Brocklebank. "Analysis of Proteinuria Using a Commercial System for Automated Electrophoresis and Isoelectric Focusing." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 25, no. 3 (May 1988): 319–24. http://dx.doi.org/10.1177/000456328802500322.

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We describe an investigation of proteinuria using Pharmacia PhastSystem™ electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.

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KOBAYASHI, Ryusuke, and Keishichiro IMAIZUMI. "Determination of Tellurium in Urine by Hydride Generation Atomic Absorption Spectrometry." Analytical Sciences 7, no. 3 (1991): 447–50. http://dx.doi.org/10.2116/analsci.7.447.

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KATO, Noriyuki, Hiroaki KUBO, and Hiroshi HOMMA. "Fluorescence Analysis of p-Hydroxymethamphetamine in Urine by Thin-Layer Chromatography." Analytical Sciences 21, no. 9 (2005): 1117–19. http://dx.doi.org/10.2116/analsci.21.1117.

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NIWA, Toshifumi, Kazuhiro FUJITA, Toko KASUYA, Shigeo IKEGAWA, and Junichi GOTO. "Enzyme Immunoassay for Ursodeoxycholic Acid 7-N-Acetylglucosaminides in Human Urine." Analytical Sciences 12, no. 4 (1996): 565–68. http://dx.doi.org/10.2116/analsci.12.565.

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Wilson, Thomas, Isabel Garcia-Perez, Joram M. Posma, Amanda J. Lloyd, Edward S. Chambers, Kathleen Tailliart, Hassan Zubair, et al. "Spot and Cumulative Urine Samples Are Suitable Replacements for 24-Hour Urine Collections for Objective Measures of Dietary Exposure in Adults Using Metabolite Biomarkers." Journal of Nutrition 149, no. 10 (June 26, 2019): 1692–700. http://dx.doi.org/10.1093/jn/nxz138.

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ABSTRACT Background Measurement of multiple food intake exposure biomarkers in urine may offer an objective method for monitoring diet. The potential of spot and cumulative urine samples that have reduced burden on participants as replacements for 24-h urine collections has not been evaluated. Objective The aim of this study was to determine the utility of spot and cumulative urine samples for classifying the metabolic profiles of people according to dietary intake when compared with 24-h urine collections in a controlled dietary intervention study. Methods Nineteen healthy individuals (10 male, 9 female, aged 21–65 y, BMI 20–35 kg/m2) each consumed 4 distinctly different diets, each for 1 wk. Spot urine samples were collected ∼2 h post meals on 3 intervention days/wk. Cumulative urine samples were collected daily over 3 separate temporal periods. A 24-h urine collection was created by combining the 3 cumulative urine samples. Urine samples were analyzed with metabolite fingerprinting by both high-resolution flow infusion electrospray mass spectrometry (FIE-HRMS) and proton nuclear magnetic resonance spectroscopy (1H-NMR). Concentrations of dietary intake biomarkers were measured with liquid chromatography triple quadrupole mass spectrometry and by integration of 1H-NMR data. Results Cross-validation modeling with 1H-NMR and FIE-HRMS data demonstrated the power of spot and cumulative urine samples in predicting dietary patterns in 24-h urine collections. Particularly, there was no significant loss of information when post-dinner (PD) spot or overnight cumulative samples were substituted for 24-h urine collections (classification accuracies of 0.891 and 0.938, respectively). Quantitative analysis of urine samples also demonstrated the relation between PD spot samples and 24-h urines for dietary exposure biomarkers. Conclusions We conclude that PD spot urine samples are suitable replacements for 24-h urine collections. Alternatively, cumulative samples collected overnight predict similarly to 24-h urine samples and have a lower collection burden for participants.

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Akbar, Arlitha, Kasmuddin Darmo, Devilda -, Kabah Paharu, and Amirah Aznawi. "ANALISIS SEDIMEN DAN KADAR PROTEIN URIN SEBAGAI SKRINING INFEKSI SALURAN KEMIH PADA IBU HAMIL." Jurnal Kebidanan Khatulistiwa 9, no. 1 (January 31, 2023): 1. http://dx.doi.org/10.30602/jkk.v9i1.1143.

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Latar Belakang: Infeksi saluran kemih (ISK) adalah jenis infeksi yang paling umum selama kehamilan dan merupakan faktor risiko penyebab ketuban pecah dini. Ketuban pecah dini adalah pecahnya selaput ketuban sebelum waktunya, insiden di Indonesia tahun 2018 sebanyak 6% dari total kelahiran. Tujuan : Apakah pemeriksaan sedimen dan protein urine pada ibu hamil dapat membantu dalam pencegahan dan penanggulangan infeksi saluran kemih secara dini Metode: Desain penelitian menggunakan deskriptif analitik dengan teknik sampling purposive sampling. Populasi sampel adalah semua ibu hamil yang memeriksakan kehamilannya di Puskesmas Minasaupa. Lokasi dan waktu penelitian dilaksanakan di Puskesmas Minasaupa Kota Makassar pada bulan Maret – April 2022. Hasil: pemeriksaan sedimen urin ibu hamil dari 25 sampel, menunjukan adanya unsur-unsur organik yaitu sel leukosit (0-5/LPB) sebanyak 6 sampel (24%) , sel leukosit (>5) sebanyak 19 sampel dengan persentase (76%) Untuk sel eritrosit (0-3/LPB) sebanyak 25 sampel (100%), dan sel epitel (+1) sebanyak 7 sampel (28%), sel epitel (2+) sebanyak 12 sampel (48%), dan sel epitel (+3) yaitu sebnyak 6 sampel (24%). selanjutnya Pada pemeriksaan kadar protein urin dari 25 sampel, 24 sampel diantaranya menunjukan hasil Negatif (-) (96%) dan 1 menunjukan hasil Positif (+) (4%). Kesimpulan: Pemeriksaan sedimen dan protein urine pada ibu hamil dapat membantu dalam pencegahan dan penanggulangan infeksi saluran kemih secara dini

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Lee, Min-Hee, Yong-Hyun Kim, Sang-Hee Jo, Si-On Choi, Inyoung Sa, and Ki-Hyun Kim. "Comparative Analysis of Offensive Odorants in Urine Samples in Relation to Sample Treatment Conditions." Journal of Korean Society for Atmospheric Environment 30, no. 5 (October 31, 2014): 492–503. http://dx.doi.org/10.5572/kosae.2014.30.5.492.

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Muhazir, Ahmad, Misbah Misbah, and Rini Puji Astutik. "IDENTIFIKASI PENYAKIT GAGAL GINJAL MELALUI BAU URINE MENGGUNAKAN SENSOR GAS DAN JARINGAN SARAF TIRUAN." E-Link: Jurnal Teknik Elektro dan Informatika 16, no. 1 (July 21, 2021): 57. http://dx.doi.org/10.30587/e-link.v16i1.2714.

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Penyakit ginjal kronik (PGK) merupakan suatu proses patofisiologi dengan etiologi beragam, mengakibatkan penurunan fungsi ginjal yang progresif dan pada umumnya berakhir dengan gagal ginjal. Penelitian ini bertujuan untuk merancang suatu alat deteksi gagal ginjal yang handal dan ekonomis, dengan mengidentifikasi penderita ginjal kronik (gagal ginjal) melalui kadar urea menggunakan reagen diasetilmonoksim akan diserap oleh urine. Hal itu dapat menimbulkan warna kuning yang mana akan dicek kadarnya dengan spektometer. Metode yang digunakan diawali dengan studi kepustakaan, metode perancangan, metode pengujian, dan analisa data. Penggunaan sensor gas diharapkan efektif dalam mengidentifikasi penderita penyakit gagal ginjal. Untuk pengidentifikasian bau urine sakit gagal ginjal dan non sakit gagal ginjal sebelumnya dilakukan proses training data dan identifikasi menggunakan backpropagation yang terdapat pasang data dan pola untuk mewakili karakterisasi bau urine diabetes dan non diabetes. Kepekaan sensor gas pada alat pendeteksi urinee menggunakan modul sensor Gas MQ-2, MQ-4, MQ-7 dan MQ-135 yang sudah dilatihkan pada system dengan memiliki akurasi 96,80% dan sensor yang lebih mendominasi ada pada MQ-2, MQ-4 untuk orang sakit dan sehat. Sehingga sistem mampu membedakan mana jenis urine orang terkena penyakit ginjal atau urine orang yang sehat.

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SUNUNTA, Suphanan, Poomrat RATTANARAT, Orawon CHAILAPAKUL, and Narong PRAPHAIRAKSIT. "Microfluidic Paper-based Analytical Devices for Determination of Creatinine in Urine Samples." Analytical Sciences 34, no. 1 (2018): 109–13. http://dx.doi.org/10.2116/analsci.34.109.

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IINUMA, Fumio, Masayoshi TABARA, Makiko SUZUKI, and Mitsuo WATANABE. "Fluorometric determination of xanthurenic acid in urine with calcium nitrate and diethylamine." Analytical Sciences 4, no. 3 (1988): 317–20. http://dx.doi.org/10.2116/analsci.4.317.

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KAWAI, Satoshi, Ryota NISHIOKA, Masaharu NAKAHIGASHI, Kazuko KONDO, and Yuzi TAKAYAMA. "Determination of 3-keto-valproate in urine by metal capillary gas chromatography." Analytical Sciences 5, no. 3 (1989): 301–4. http://dx.doi.org/10.2116/analsci.5.301.

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HASHIZUME, Takeshi, Wataru TANIGUCHI, and Tamiji SUGIYAMA. "Mass spectrometric determination of N6-isopentenyladenosine and N6-isopentenyladenine from human urine." Analytical Sciences 2, no. 2 (1986): 157–59. http://dx.doi.org/10.2116/analsci.2.157.

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FAN, Jing, Yahong CHEN, Suling FENG, Cunling YE, and Jianji WANG. "Flow-Injection Spectrophotometric Determination of Sulfadiazine and Sulfamethoxazole in Pharmaceuticals and Urine." Analytical Sciences 19, no. 3 (2003): 419–22. http://dx.doi.org/10.2116/analsci.19.419.

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Monferdini, Donna, Margaret Joinville, and William Grove. "Improving Urine Sediment Analysis." Laboratory Medicine 26, no. 10 (October 1, 1995): 660–64. http://dx.doi.org/10.1093/labmed/26.10.660.

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Journal articles on the topic 'Urine analysi'

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