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1

Vaidya, A. M. "Ultrafiltration modelling." Thesis, University of Manchester, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237688.

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2

Rakotoarisoa, Herivola Henri. "Influence d'un promoteur de turbulence fluidisé en ultrafiltration et électro-ultrafiltration." Grenoble INPG, 1986. http://www.theses.fr/1986INPG0107.

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Les membranes utilisees sont des membranes minerales. Pour l'ultrafiltration, la fluidisation permet d'ameliorer notablement les debits de permeation sans qu'il y ait pour autant deterioration de la retention en macromolecules. Mise en evidence de conditions optimales de porosite du lit fluidise. L'action des particules fluidisees est moins significative pour l'electroultrafiltration
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3

Ghosh, Raja. "Protein fractionation using ultrafiltration." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302133.

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4

BANZI, ALBERT. "Concentration de lactoserum par ultrafiltration." Toulouse 3, 1986. http://www.theses.fr/1986TOU30042.

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Ce travail concerne la conception et le controle d'installations destinees a concentrer du lactoserum doux par ultrafiltration sur membranes minerales. Une etude bibliographique met en evidence la necessite d'etablir des lois reliant le transfert de matiere aux conditions operatoires (pression, regime hydrodynamique, concentration, geometrie de membrane). Ces lois sont etablies empiriquement en utilisant divers lactoserums et des solutions de concentrats de proteines. Deux methodologies experimentales (pression constante ou pression variable, a concentration constante) sont envisagees. Enfin,
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5

Banzi, Albert. "Concentration du lactosérum par ultrafiltration." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb37595695f.

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6

Farzanehsa, Seyedeh Zahra. "Decolourisation of Molasses by Ultrafiltration." Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/18596.

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Molasses is a dark brown by-product of sugar production and refining, rich in sugar, vitamins, and salts. The dark coloured pigments are mainly melanoidins, which are produced through the Maillard reaction between amino acids and sugar. Molasses is widely used as an inexpensive source of sugar in biorefineries to produce a range of products including baker’s yeast. Due to adsorption of melanoidins, Baker’s yeast has a dark colour and needs to be washed in several steps to be acceptable to the market. This washing increases the volume of wastewater, which causes major wastewater treatment i
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7

Ramli, Nor Hanuni. "Ultrafiltration of polydisperse colloidal silica." Thesis, Swansea University, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678552.

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8

Demessie, Berhanu. "Ultrafiltration of Partially Degraded Starch Solution." Doctoral thesis, Norwegian University of Science and Technology, Faculty of Natural Sciences and Technology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-30.

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<p>Desizing wastewater is largely responsible for the chemical oxygen demand (COD) load in the textile industry wastewater. A larger portion of COD comes from degraded starch in desizing wastewater. Removing the starch from the wastewater by an ultrafiltration process may reduce the environmental problem caused by the textile factory. If the treatment is made in such a way that all starch components are removed from the wastewater, the treated water can be reused by the factory. If the starch in the concentrate is stable, it can also be reused as a sizing agent. This will give the factory an e
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9

Turkson, Abraham K. "Electro-ultrafiltration with rotating dynamic membranes." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=72036.

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In axial electrofiltration, a DC electric field is imposed between a rotating inner cylinder and a stationary outer cylinder giving rise to four mechanisms which act to minimize solute accumulation at the filter surface: turbulence, centrifugal force, electrophoresis and shear stress which removes solute aggregates.<br>Four dynamic membranes, Zr(IV) oxide, calcium oleate, poly-2-vinylpyridine and cadmium sulfide, were used to filter bovine serum albumin (BSA) in a disodium phosphate solution at pH = 8 and Prussian blue in distilled water. Prussian blue is a particle of 0.01(mu)m diameter with
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10

Vasan, S. S. "Analysis of mass transfer in ultrafiltration." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424738.

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11

Cao, X. "Colloid and interface aspects of ultrafiltration." Thesis, Swansea University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636202.

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Osmotic pressure and diffusion coefficients were studied experimentally and theoretically in order to facilitate fully quantitative descriptions of ultrafiltration processes. These parameters may be incorporated in the solution of the equations for hydrodynamics and mass transfer in the filtration module. Theoretical descriptions are presented to allow calculation of osmotic pressure, taking account of the electrostatic repulsive interactions, London Van Der Waals forces, configurational entropy and additional interactions of unknown source. Approaches of constant zeta potential and charge reg
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12

Williams, P. M. "Protein ultrafiltration : a colloidal interaction approach." Thesis, Swansea University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636643.

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The influence of the physicochemical conditions on the permeation rate in dead-end and cross flow ultrafiltration of protein dispersions is investigated. The rigorous mathematical models developed are based on sophisticated descriptions of the protein-protein interactions within the layer close to the membrane surface which is responsible for controlling the permeation rate. The calculations are expressed in terms of an osmotic pressure which accounts for the multiparticle electrostatic interactions, dispersion forces and configurational entropy. Electrostatic interactions are accounted for by
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13

Dodd, P. W. "Measurement of velocity profiles in ultrafiltration." Thesis, Imperial College London, 1986. http://hdl.handle.net/10044/1/37995.

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14

Ghaffor, Noreddine. "Ultrafiltration tangentielle de suspensions mixtes : hydrocarbures." Montpellier 2, 1995. http://www.theses.fr/1995MON20081.

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L'eau residuaire de raffinerie petroliere traitee contient un melange d'hydrocarbures (hc) et de matieres en suspension (mes) resultant principalement d'un traitement biologique. L'ultrafiltration (uf) semblerait etre en mesure de resoudre le probleme de rejets en respectant les normes europeennes. La principale limitation economique resulte de la densite de flux de permeat qui doit etre suffisamment elevee pour minimiser la surface filtrante. Le but de ce travail est l'identification des mecanismes de limitation de transfert de matiere. La premiere etape a ete l'identification d'une membrane
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15

Lentsch, Sandrine. "Ultrafiltration et électro-ultrafiltration : Mécanismes impliqués dans le fractionnement des mélanges albumine-lactoferrine et albumine-poly(éthylène glycol)." Toulouse 3, 1993. http://www.theses.fr/1993TOU30091.

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L'analyse des potentialites de fractionnement de melanges de macromolecules par ultrafiltration et electro-ultrafiltration est basee sur l'etude des melanges albumine-lactoferrine et albumine-poly(ethylene glycol). L'etude du premier melange montre que la transmission d'une proteine a travers une membrane depend de l'exclusion electrostatique et sterique. Elle met egalement en evidence des conditions de ph et de force ionique pour lesquelles les interactions lactoferrine-lactoferrine et lactoferrine-albumine modifient considerablement les mecanismes qui controlent habituellement la selectivite
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16

Priyananda, Pramith School of Chemical Engineering &amp Industrial Chemistry UNSW. "Protein and fatty acid interactions during ultrafiltration." Awarded by:University of New South Wales. School of Chemical Engineering and Industrial Chemistry, 2006. http://handle.unsw.edu.au/1959.4/24310.

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Proteins and fatty acids often exist in solutions containing biological matter that are treated with membranes. These proteins and fatty acids interact with each other as well as with the membranes thereby affecting the flux. Binding of fatty acids to proteins results in complexes that are much larger than fatty acid molecules. Exploitation of this size difference to remove difficult to separate fatty acids from aqueous solutions by ultrafiltration was investigated in this study. In addition, the fouling of membrane by the protein-fatty acid mixtures containing free dissolved fatty acids was s
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17

Wan, Yinhua. "Fractional biological macromolecules using carrier phase ultrafiltration." Thesis, University of Oxford, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409749.

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18

Jenner, F. "Dynamic ultrafiltration models for charged colloidal dispersions." Thesis, Swansea University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637422.

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The development of quantitative predictive models is of great significance for the successful application of membrane separation processes in the process industries. Successful models exist for the ultrafiltration of macromolecules and particles with sizes 2 to 5nm and 500nm to 10μm, respectively. None of the approaches could predict colloidal ultrafiltration rates in the intermediate size range of 5 to 500nm. This is due to interparticle interactions, especially electrostatic interactions which play an important role and which have been widely neglected or even overlooked by many membrane sci
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19

Aimar, Pierre. "Mécanismes de transfert de matière en ultrafiltration." Toulouse 3, 1987. http://www.theses.fr/1987TOU30166.

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On etudie l'ultrafiltration de fluides contenant des proteines et plus particulierement du lait et de ses derives. On etudie plus precisement le phenomenes de diffusion d'encrassement et de colmatage de la membrane. On observe le flux limite de solute en fonction de la polarisatiuon, de la concentration et des proprietes rheologiques des solutions proteiques utilisees
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20

Heran, Marc. "La rétrofiltration Haute Fréquence et micro/ultrafiltration." Montpellier 2, 2000. http://www.theses.fr/2000MON20096.

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Cette etude est consacree a la retrofiltration haute frequence et ses applications economiquement importantes. Dans un premier temps, la microfiltration d'une suspension de bentonite, a travers une membrane ceramique tubulaire de 0,2 m, a valide le montage par l'obtention de flux de l'ordre de 900 l. H - 1. M - 2. L'amortissement interne a ete quantifie par un montage specifique et par l'observation des regimes transitoires. Une etude systematique a permis de determiner l'influence de chaque variable operatoire. La retrofiltration n'empechant pas une decroissance du flux vers un equilibre cine
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21

Koh, Chen Ning. "Microfiltration and ultrafiltration with high frequency backpulsing /." Aachen : Shaker, 2009. http://d-nb.info/995622019/04.

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22

Aimar, Pierre. "Mécanismes de transfert de matière en ultrafiltration." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37602128h.

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23

Ayre, Lorna M. "The adsorption of proteins onto ultrafiltration membranes." Thesis, Loughborough University, 2000. https://dspace.lboro.ac.uk/2134/35236.

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The mass of five proteins (Bovine serum albumin (BSA), casein, lysozyme, ovalbumin and pepsin) adsorbed to five different membrane materials (of various hydrophobicities) was quantified using a static system and analysed to establish any trends. Comparing the results from the five membranes it seems that there were no obvious trends between the protein masses adsorbed indicating that it may not be just one aspect of protein structure that is important in the adsorption process. Many investigations have indicated that the protein may undergo a conformational change during the adsorption process
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24

Finnigan, Sean Matthew. "Pulsed flow ultrafiltration in baffled tubular membranes." Thesis, University of Bath, 1990. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254875.

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25

Bastian, Eric Douglas. "Ultrafiltration: Retentate-Permeate Partititioning of Milk Constituents." DigitalCommons@USU, 1987. https://digitalcommons.usu.edu/etd/5335.

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The effect of ultrafiltration, diafiltration, and preacidification of milk on the partition of specific milk components between retentate and permeate was studied. Percent retention (for any component Y) was determined as: [ l - (%Y in soln. permeate/%Y in soln. retentate)] X 100 where %Y in soln. = [%Y/(%Y + %H 20)] Simultaneous samples of retentate and permeate were taken at several points during each process. Percent retention of total solids, fat, total protein, rennet clottable nitrogen, lactose, total calcium, ionic calcium, sodium, phosphorous, and riboflavin was determined at each samp
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26

MEHMOOD, MIAN FARRUKH. "UV cured polysulfone based membranes for ultrafiltration." Doctoral thesis, Politecnico di Torino, 2018. http://hdl.handle.net/11583/2708562.

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Membrane technology has acquired significant importance in a variety of applications such as water treatment, health sector and food industry. Nevertheless there is a growing demand of more functional and stable membranes. This thesis is focused on improving solvent stability of polysulfone based membranes without compromising the flux properties. Polysulfone (PSF) is a widely used membrane material for ultrafiltration process because PSF can be easily fabricated into highly porous structure via non–solvent induced phase separation (NIPS). Such a structure is imperative for ultrafiltration pr
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27

Wlodarczyk, Damien. "Elaboration contrôlée de membranes à base de chitosane pour le traitement de l'eau." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS271/document.

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Le travail de thèse présenté dans ce manuscrit a pour objectif de mettre en place un nouveau procédé d’élaboration de membranes à base de chitosane pour le traitement d’effluents acides contenants des ions métallique. Soluble en milieu aqueux acide, le chitosane présente la propriété de gélifier lorsque le pH devient basique, ce qui permet d’envisager l’élaboration de membrane sans solvant organique contrairement aux polymères synthétiques classiques. Par ailleurs, ce travail de thèse s’est intéressé à un procédé original de gélification par voie enzymatique, dans lequel la gélification in-sit
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28

Hanon, Emmanuel. "Electro-ultrafiltration appliquée à l'épuration ou à la concentration d'ions et au décolmatage." Pau, 1998. http://www.theses.fr/1998PAUU3006.

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Le procede d'ultrafiltration est limite dans son utilisation par ses caracteristiques propres. Ainsi il est impossible de pretendre a un transport selectif d'ions de petites tailles. D'autre part, lors d'une filtration d'un fluide contenant des macrosolutes, il se forme un encrassement fortement penalisant au niveau des flux de permeat. Le double objectif presente dans ce memoire, repond a ces limitations en utilisant le procede d'electro-ultrafiltration. A partir du pilote que nous avons realise, nous mettons en evidence qu'il est possible d'obtenir une epuration ou une concentration de solut
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29

Habarou, Hélène. "Suivi et caractérisation du colmatage par les matières organiques des membranes de microfiltration et d'ultrafiltration : application à la production d'eau potable." Poitiers, 2004. http://www.theses.fr/2004POIT2290.

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Cette étude traite du colmatage des membranes basse pression par les matières organiques des eaux naturelles. Le premier objectif a été d'évaluer la capacité d'unités pilotes à apprécier le potentiel colmatant. Ces unités sont alimentées par de l'eau renouvelée quotidiennement ce qui permet de se rapprocher des conditions d'exploitation sur usine. Les profils de perte de flux enregistrés sur plusieurs campagnes d'essais sur site ont permis de valider l'intérêt de notre outil expérimental. Le second objectif portait sur la caractérisation du colmatage de nature organique. Une grande diversité d
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30

Luk, Chi Hang Lucas. "Preparation and characterization of ultrafiltration membranes fabricated from poly(amidesulfonamide)s and their possible uses in separation process." HKBU Institutional Repository, 1999. http://repository.hkbu.edu.hk/etd_ra/250.

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31

Mondor, Martin. "Étude de l'influence de la pression transmembranaire dans un module de microfiltration." Sherbrooke : Université de Sherbrooke, 2000.

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32

Reinhart, Ralf. "Aufbereitung der Extraktphase mittels Ultrafiltration bei der Schwermetallextraktion." Doctoral thesis, Technische Universitaet Bergakademie Freiberg Universitaetsbibliothek &quot;Georgius Agricola&quot, 2009. http://nbn-resolving.de/urn:nbn:de:swb:105-6499063.

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Ziel der Arbeit ist die Beschreibung des Membranverhaltens bzw. der Membraneigenschaften bei der Regeneration von Extraktionsmitteln der Metallextraktion durch Trennung von beladenen und unbeladenen Extraktionsmitteln mittels Ultrafiltration. Dazu wurden die Trennleistungen als Funktion von Druck, Temperatur und Solventzusammensetzung für verschiedene Membranmaterialien und -typen untersucht. Anhand von Sorptionsversuchen konnte die Löslichkeit der Komponenten der organischen Phase in den verschiedenen Polymermatrizen von Membranen aus regenerierter Cellulose bzw. Polyethersulfon und die adsor
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33

Gwicana, Sakumzi. "Micellar-enhanced ultrafiltration of palladium and platinum anions." Thesis, Nelson Mandela Metropolitan University, 2007. http://hdl.handle.net/10948/518.

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The project was concerned with studying the capability of a micellar-enhanced ultrafiltration system (MEUF) to remove platinum group metal ions namely Pt (lV) and Pd (ll) chloro anions from aqueous industrial waste effluents. South Africa has the world’s largest reserves of platinum group metals (PGMs) and other valuable metals such as manganese, chrome ores, titanium minerals etc. which are required for new automotive and other technologies, including fuel cells, catalytic converters and lighter components. The consistent loss with the industrial waste stream and the toxicological effects of
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34

Porcelli, Nicandro. "Chemical cleaning of potable microfiltration and ultrafiltration membranes." Thesis, Cranfield University, 2009. http://dspace.lib.cranfield.ac.uk/handle/1826/4659.

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Concerns over possible waterborne disease forced drinking water supply companies in England and Wales to adopt microfiltration and ultrafiltration technologies rapidly. MF and UF membrane plants are designed to produce water of a consistent quality regardless of throughput and fluctuations in the feedwater quality. To operate well they need to maintain flux and balance the rate of fouling, and chemical cleaning performance is critical to this. Giant steps have been taken into characterizing the foulants scientifically in the last few years while cleaning is reactive and ad hoc. This thesis exp
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35

Lu, Junren. "Separation of proteins from Chicken Eggs using ultrafiltration." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509984.

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36

Aguinaldo, Jorge T. "Precipitative Softening and Ultrafiltration Treatment of Beverage Water." Scholar Commons, 2006. http://scholarcommons.usf.edu/etd/3895.

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Lime softening, chlorination, clarification and filtration have been long recognized treatment processes for beverage water specifically the carbonated soft drink (CSD) because it provides consistent water quality required for bottling plants, however these processes are becoming uneconomical and causes more problems than the benefits they offer. These processes require very large foot print, occupy large plant volume, and generate large volume of sludge which causes disposal problems. Chlorination produces trihalomethanes (THMs) and other by-products which are detrimental to health and impart
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37

Hill, David M. "Size fractionation of metals in rivers by ultrafiltration." Thesis, University of Newcastle Upon Tyne, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307892.

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38

Ilahiane, Lahcen. "Ultrafiltration par membranes ioniques polysulfone-sulfonée : caractérisation, utilisation." Lyon 1, 1985. http://www.theses.fr/1985LYO10510.

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39

Pierre-Collet, Gwenaëlle. "Rétention de virus en ultrafiltration : protocole de caractérisation." Toulouse 3, 2010. http://thesesups.ups-tlse.fr/986/.

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Le problème abordé dans ce travail est celui de la mise au point d'un protocole raisonné de détermination de l'abattement du nombre de virus par une membrane de filtration, dans des conditions proches de celles que l'on rencontre dans le domaine du traitement de l'eau. Pour cela, ont été recherchées parmi les conditions opératoires, celles qui conduisent au transfert le plus important, de manière à révéler d'éventuelles faiblesses dans les matériaux testés. Au cours de cette étude, les phages MS2 et Qbeta ont été choisis comme indicateurs. Leurs comportements en suspension dans divers solvants
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40

Neri, Mauro. "A wearable mechatronic device for extracorporeal blood ultrafiltration." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3422200.

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The interest in the design of portable and wearable medical devices is related to both the relevant clinical and social benefits for patients and the potential economic savings for national health services. Biomedical technologies are improving at a very fast rate and represent an extraordinary means to develop innovative portable and wearable devices which can help people live in a prosperous way, in particular reducing sorrow in case of disease. This leads to a widespread effort to develop devices which can execute at home therapies that are usually performed in hospitals. This thesis pres
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Campbell, Moray James. "Novel membrane separations in biotechnology." Thesis, University of Kent, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359337.

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42

Manno, Peggy. "Intérêt des vortex de dean en filtration par membranes : application à des suspensions de levures et à la bière." Toulouse 3, 1998. http://www.theses.fr/1998TOU30264.

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Ce memoire est une contribution a l'etude d'une nouvelle technique de lutte contre le colmatage en filtration tangentielle. Le principe repose sur l'utilisation d'ecoulements secondaires, appeles vortex de dean, qui apparaissent dans un module helicoidal ou des fibres creuses sont enroulees en helice. Dans une premiere partie, l'analyse de l'evolution du colmatage des membranes lors de l'ultrafiltration de suspensions de levures de boulangerie fait apparaitre l'importance des differentes fractions constituant la suspension. Le colmatage irreversible (adsorption et formation d'un gel) est attri
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43

Kochan, Jozef [Verfasser]. "Layer-by-Layer Engineering of Ultrafiltration Membranes / Jozef Kochan." Aachen : Shaker, 2013. http://d-nb.info/1051575796/34.

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44

Zondervan, Edwin. "Intermediate to long term optimization of dead-end ultrafiltration." [S.l. : Groningen : s.n. ; University Library Groningen] [Host], 2007. http://irs.ub.rug.nl/ppn/304684155.

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Garcia, Diez Leticia. "Mizellare Chromatographie und selektive Ultrafiltration zur Trennung von Aminosäureracematen /." Aachen : Shaker, 2008. http://d-nb.info/98929837X/04.

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46

Gélinas, Stéphanie. "Micellar-enhanced ultrafiltration using a twin-head cationic surfactant." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23262.

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In Micellar-Enhanced Ultrafiltration (MEUF), a surfactant is added to a wastewater stream containing an organic solute. Most of the solute is solubilized in micelles, which are aggregates of surfactant molecules, formed in the feed solution. Separation is achieved through retention of the micelles by an ultrafiltration membrane. Since much of the solute is within the micelles, a membrane with a molecular weight cut off (MWCO) much larger than the molecular weight of the solute is used. Although some unsolubilized solute and surfactant monomers pass through the membrane, the use of a surfactant
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47

Bellara, Sanjay R. "Novel developments in the application of ultrafiltration for bioseparations." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284339.

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48

Jain, M. "Investigations into electrostatic interactions during ultrafiltration of (bio) colloids." Thesis, Swansea University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637386.

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The influence of physiochemical conditions on the fouling and fractionation of charged solutes, particularly proteins, during ultrafiltration has been investigated in this study. There exists great scope for improvement of ultrafiltration selectivity and efficiency through manipulation of pH, ionic strength and pressure. With a knowledge of the inherent physiochemical properties of the (bio)colloids and membranes one can apply colloid science insights to model interactions taking place a the membrane surface. Charged solutes are driven towards the membrane pores by hydrodynamic forces. As they
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49

Drumond, de Sousa Maria Cláudia Ferreira. "Ultrafiltration in renal glomerular capillaries : theoretical effects of ultrastructure." Thesis, Massachusetts Institute of Technology, 1994. http://hdl.handle.net/1721.1/32131.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1994.<br>Includes bibliographical references (leaves 244-254).<br>by Maria Cláudia Ferreira Drumond de Sousa.<br>Ph.D.
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50

Shugman, Emad Musbah. "Cleaning of micro- and ultrafiltration membranes with infrasonic backpulsing." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/3053.

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Thesis (MScEng (Process Engineering))--University of Stellenbosch, 2009.<br>ENGLISH ABSTRACT: Membrane fouling is universally considered to be one of the most critical problems in the wider application of membranes in filtration separation. Fouling is caused by the deposition of particles not only on the surface of the membrane, but also inside the membrane pores, which reduces permeate flux and leads to a reduction of the efficiency and the longevity of the membrane. The backpulsing cleaning method can be used to remove deposited foulants from the surface of the membrane, without having
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