Relevant bibliographies by topics / Typhimurium

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Academic literature on the topic 'Typhimurium'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 September 2023

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Journal articles on the topic "Typhimurium"

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Dunstan, Sarah J., Cameron P. Simmons, and Richard A. Strugnell. "Use of In Vivo-Regulated Promoters To Deliver Antigens from Attenuated Salmonella enterica var. Typhimurium." Infection and Immunity 67, no. 10 (October 1, 1999): 5133–41. http://dx.doi.org/10.1128/iai.67.10.5133-5141.1999.

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ABSTRACT This study describes the construction and analysis of three in vivo-inducible promoter expression plasmids, containing pnirB, ppagC, and pkatG, for the delivery of foreign antigens in the ΔaroAD mutant ofSalmonella enterica var. Typhimurium (hereafter referred to as S. typhimurium). The reporter genes encoding β-galactosidase and firefly luciferase were used to assess the comparative levels of promoter activity in S. typhimuriumin vitro in response to different induction stimuli and in vivo in immunized mice. It was determined that the ppagC construct directed the expression of more β-galactosidase and luciferase inS. typhimurium than the pnirB and pkatG constructs, both in vitro and in vivo. The gene encoding the C fragment of tetanus toxin was expressed in thearoAD mutant of S. typhimurium (BRD509) under the control of the three promoters. Mice orally immunized with attenuated S. typhimurium expressing C fragment under control of the pagC promoter [BRD509(pKK/ppagC/C frag)] mounted the highest tetanus toxoid-specific serum antibody response. Levels of luciferase expression in vivo and C-fragment expression in vitro from thepagC promoter appeared to be equivalent to if not lower than the levels of expression detected with the constitutivetrc promoter. However, mice immunized with BRD509(pKK/ppagC/C frag) induced significantly higher levels of tetanus toxoid-specific antibody than BRD509(pKK/C frag)-immunized mice, suggesting that the specific location of foreign antigen expression may be important for immunogenicity. Mutagenesis of the ribosome binding sites (RBS) in the three promoter/C fragment expression plasmids was also performed. Despite optimization of the RBS in the three different promoter elements, the expression levels in vivo and overall immunogenicity of C fragment when delivered to mice by attenuated S. typhimurium were not affected. These studies suggest that in vivo-inducible promoters may give rise to enhanced immunogenicity and increase the efficacy of S. typhimuriumas a vaccine vector.
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Goh, Yun Shan, Simon Clare, Francesca Micoli, Allan Saul, Pietro Mastroeni, and Calman A. MacLennan. "Monoclonal Antibodies of a Diverse Isotype Induced by an O-Antigen Glycoconjugate Vaccine MediateIn VitroandIn VivoKilling of African Invasive Nontyphoidal Salmonella." Infection and Immunity 83, no. 9 (July 13, 2015): 3722–31. http://dx.doi.org/10.1128/iai.00547-15.

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NontyphoidalSalmonella(NTS), particularlySalmonella entericaserovars Typhimurium and Enteritidis, is responsible for a major global burden of invasive disease with high associated case-fatality rates. We recently reported the development of a candidate O-antigen–CRM197glycoconjugate vaccine againstS.Typhimurium. Here, using a panel of mouse monoclonal antibodies generated by the vaccine, we examined the relative efficiency of different antibody isotypes specific for the O:4 antigen ofS. Typhimurium to effectin vitroandin vivokilling of the invasive AfricanS. Typhimurium strain D23580. All O:4-specific antibody isotypes could mediate cell-free killing and phagocytosis ofS. Typhimurium by mouse blood cells. Opsonization ofSalmonellawith O:4-specific IgA, IgG1, IgG2a, and IgG2b, but not IgM, resulted in cell-dependent bacterial killing. At high concentrations, O:4-specific antibodies inhibited both cell-free complement-mediated and cell-dependent opsonophagocytic killing ofS. Typhimuriumin vitro. Using passive immunization in mice, the O:4-specific antibodies providedin vivofunctional activity by decreasing the bacterial load in the blood and tissues, with IgG2a and IgG2b being the most effective isotypes. In conclusion, an O-antigen–CRM197glycoconjugate vaccine can induce O-antigen-specific antibodies of different isotypes that exertin vitroandin vivokilling ofS. Typhimurium.
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Abd El Ghany, Moataz, Angela Jansen, Simon Clare, Lindsay Hall, Derek Pickard, Robert A. Kingsley, and Gordon Dougan. "Candidate Live, Attenuated Salmonella enterica Serotype Typhimurium Vaccines with Reduced Fecal Shedding Are Immunogenic and Effective Oral Vaccines." Infection and Immunity 75, no. 4 (February 12, 2007): 1835–42. http://dx.doi.org/10.1128/iai.01655-06.

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ABSTRACT Environmental shedding of genetically manipulated microorganisms is an issue impeding the development of new live vaccines. We have investigated the immunogenicity of a number of novel Salmonella enterica serotype Typhimurium oral vaccine candidates that express the fragment C (TetC) component of tetanus toxin and harbor combinations of additional mutations in genes shdA, misL, and ratB that contribute to the persistence of serotype Typhimurium's colonization of the intestine. Serotype Typhimurium aroA (TetC) derivatives harboring additional mutations in either shdA or misL or combinations of these mutations exhibited a marked decrease in shedding of the vaccine strain in the feces of orally vaccinated mice. However, equivalent levels of anti-TetC and anti-Salmonella lipopolysaccharide immunoglobulin G (IgG), IgG1, IgG2a, and IgA were detected in sera of the vaccinated but not of the control mice. Cellular immune responses to TetC were detected in all vaccinated mice, regardless of the presence of the additional mutations in shdA or misL. Further, immunization with serotype Typhimurium aroA candidate vaccines harboring shdA and misL afforded complete protection against challenge with a virulent strain of serotype Typhimurium.
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AL-kafaji, Narjis Amer, Zainab R. Zghair, and MethaqGhlibAbd AL-Rubaie. "Pathological study of alcoholic plant agolanceolata crude extracts effect on some Salmonella species invitro and in vivo." Kufa Journal For Veterinary Medical Sciences 7, no. 1B (October 10, 2016): 109–16. http://dx.doi.org/10.36326/kjvs/2016/v7i1b4272.

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This study was designed to explore the pathological effect of alcoholic plant agolanceolata crude extracts effect on some Salmonella species invitro and in vivo study by using laboratory mice .plant agolanceolata crude extract were dealing in vitro study against some virulent bacteria including Salmonella typhimurium ,Salmonella typhiond Salmonella hadar. And using the concentrations (100,150,200,250 and 300) mg for plan tagolanceolata crude extract ,concentration at 300 mg and the highest inhibitory effect on the three types of salmonella ,when compared with the other concentrations .The pathological study of plant agolanceolata crude extract was done against the infection of Salmonella typhimurium as in vivo study by using white laboratory mice. Twenty four mice were randomly divided into six groups, each group contain four animals. First group was administrated orally o.3 ml of Salmonella typhimuriumof bacterial suspension containing 1x106cfu orally for one week of infection. Second group administrated orally with 0.3 ml of bacterial suspension containing 1x106cfu orally of Salmonella typhimurium for two weeks as infection. Third group administrated orally with 0.3 ml of bacterial suspension containing 1x106cfu orally Salmonella typhimurium for three weeks as infection. Fourth group was administrated orally with o.3 ml of plant agolanceolata extract for one week daily after infection with Salmonella typhimurium. Fifth group was administrated orally with o.3 ml of plant agolanceolata extract for two weeks daily after infection with Salmonella typhimurium. And sixth group was administrated orally with o.3 ml of plant agolanceolata extract for three weeks daily after infection with Salmonella typhimurium. The histopathological study showed pathological changes in theintestine of the first , second and third groups that infected with Salmonella typhimuriumbacteria, (sixfhgroup)no clear pathological changes were reported except some lesions. For all, the plantagolanceolata extract apparently has therapeutic effect whenused in high concentration invitro and for long time invivo.
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Everest, Paul, Julian Ketley, Simon Hardy, Gill Douce, Shahid Khan, Jacqui Shea, David Holden, Duncan Maskell, and Gordon Dougan. "Evaluation of Salmonella typhimuriumMutants in a Model of Experimental Gastroenteritis." Infection and Immunity 67, no. 6 (June 1, 1999): 2815–21. http://dx.doi.org/10.1128/iai.67.6.2815-2821.1999.

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ABSTRACT Salmonella typhimurium strains harboring independent, defined mutations in aroA, invA,ssrA, or msbB were assessed for their ability to induce fluid accumulation, tissue damage, and local inflammation in rabbit ileal loops. Three wild-type strains of S. typhimurium, TML, HWSH, and SL1344, and two mutant strains,S. typhimurium SL1344 ssrA and S. typhimurium SL1344 msbB, consistently induced fluid accumulation in the lumen of loops and inflammation of loop-associated tissues. In contrast, three different S. typhimurium aroAstrains and an invA mutant of SL1344 did not induce significant fluid accumulation in the rabbit ileal loops. However, theS. typhimurium aroA strains did induce an inflammatory infiltrate and some local villus-associated damage, but theinvA mutant did not. Histologically, wild-type S. typhimurium, S. typhimurium SL1344 ssrA, and S. typhimurium SL1344 msbB demonstrated more severe effects on villus architecture than S. typhimurium aroA strains, whereas S. typhimurium invA-infected loops showed no detectable damage. This suggests that villus damage most likely contributes to fluid accumulation within the loop.
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Voedisch, Sabrina, Christian Koenecke, Sascha David, Heike Herbrand, Reinhold Förster, Mikael Rhen, and Oliver Pabst. "Mesenteric Lymph Nodes Confine Dendritic Cell-Mediated Dissemination of Salmonella enterica Serovar Typhimurium and Limit Systemic Disease in Mice." Infection and Immunity 77, no. 8 (June 8, 2009): 3170–80. http://dx.doi.org/10.1128/iai.00272-09.

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ABSTRACT In humans with typhoid fever or in mouse strains susceptible to Salmonella enterica serovar Typhimurium (S. Typhimurium) infection, bacteria gain access to extraintestinal tissues, causing severe systemic disease. Here we show that in the gut-draining mesenteric lymph nodes (MLN), the majority of S. Typhimurium-carrying cells show dendritic-cell (DC) morphology and express the DC marker CD11c, indicating that S. Typhimurium bacteria are transported to the MLN by migratory DCs. In vivo FLT-3L-induced expansion of DCs, as well as stimulation of DC migration by Toll-like receptor agonists, results in increased numbers of S. Typhimurium bacteria reaching the MLN. Conversely, genetically impaired DC migration in chemokine receptor CCR7-deficient mice reduces the number of S. Typhimurium bacteria reaching the MLN. This indicates that transport of S. Typhimurium from the intestine into the MLN is limited by the number of migratory DCs carrying S. Typhimurium bacteria. In contrast, modulation of DC migration does not affect the number of S. Typhimurium bacteria reaching systemic tissues, indicating that DC-bound transport of S. Typhimurium does not substantially contribute to systemic S. Typhimurium infection. Surgical removal of the MLN results in increased numbers of S. Typhimurium bacteria reaching systemic sites early after infection, thereby rendering otherwise resistant mice susceptible to fatal systemic disease development. This suggests that the MLN provide a vital barrier shielding systemic compartments from DC-mediated dissemination of S. Typhimurium. Thus, confinement of S. Typhimurium in gut-associated lymphoid tissue and MLN delays massive extraintestinal dissemination and at the same time allows for the establishment of protective adaptive immune responses.
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Gao, Yuan, Kaifeng Chen, Runshan Lin, Xuebin Xu, Fengxiang Xu, Qijie Lin, Yaping Hu, et al. "High Levels of Antibiotic Resistance in MDR-Strong Biofilm-Forming Salmonella Typhimurium ST34 in Southern China." Microorganisms 11, no. 8 (August 3, 2023): 2005. http://dx.doi.org/10.3390/microorganisms11082005.

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Salmonella enterica subsp. enterica serovar Typhimurium (S. typhimurium) is an important zoonotic pathogen with important public health significance. To understand S. typhimurium’s epidemiological characteristics in China, multi-locus sequence typing, biofilm-forming ability, antimicrobial susceptibility testing, and resistant genes of isolates from different regions and sources (human, food) were investigated. Among them, ST34 accounted for 82.4% (243/295), with ST19 ranking second (15.9%; 47/295). ST34 exhibited higher resistance levels than ST19 (p < 0.05). All colistin, carbapenem, and ciprofloxacin-resistant strains were ST34, as were most cephalosporin-resistant strains (88.9%; 32/36). Overall, 91.4% (222/243) ST34 isolates were shown to have multidrug resistance (MDR), while 53.2% (25/47) ST19 isolates were (p < 0.05). Notably, 97.8% (45/46) of the MDR-ACSSuT (resistance to Ampicillin, Chloramphenicol, Streptomycin, Sulfamethoxazole, and Tetracycline) isolates were ST34, among which 69.6% (32/46) of ST34 isolates were of human origin, while 30.4% (14/46) were derived from food (p < 0.05). Moreover, 88.48% (215/243) ST34 showed moderate to strong biofilm-forming ability compared with 10.9% (5/46) ST19 isolates (p < 0.01). This study revealed the emergence of high-level antibiotic resistance S. typhimurium ST34 with strong biofilm-forming ability, posing concerns for public health safety.
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Farn, Jacinta, and Mark Roberts. "Effect of Inactivation of the HtrA-Like Serine Protease DegQ on the Virulence of Salmonella enterica Serovar Typhimurium in Mice." Infection and Immunity 72, no. 12 (December 2004): 7357–59. http://dx.doi.org/10.1128/iai.72.12.7357-7359.2004.

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ABSTRACT DegQ is a serine protease that is highly homologous to HtrA, an important virulence determinant of Salmonella enterica serovar Typhimurium. We examined if DegQ is involved in serovar Typhimurium pathogenesis. A serovar Typhimurium degQ mutant was as virulent as the wild-type strain in mice. However, a serovar Typhimurium htrA degQ mutant survived less well in murine organs, particularly in the liver, than a serovar Typhimurium htrA mutant. DegQ is not essential for serovar Typhimurium pathogenesis but may play a small role during salmonella growth at systemic sites.
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Luk, Chak Hon, Camila Valenzuela, Magdalena Gil, Léa Swistak, Perrine Bomme, Yuen-Yan Chang, Adeline Mallet, and Jost Enninga. "Salmonella enters a dormant state within human epithelial cells for persistent infection." PLOS Pathogens 17, no. 4 (April 30, 2021): e1009550. http://dx.doi.org/10.1371/journal.ppat.1009550.

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Salmonella Typhimurium (S. Typhimurium) is an enteric bacterium capable of invading a wide range of hosts, including rodents and humans. It targets different host cell types showing different intracellular lifestyles. S. Typhimurium colonizes different intracellular niches and is able to either actively divide at various rates or remain dormant to persist. A comprehensive tool to determine these distinct S. Typhimurium lifestyles remains lacking. Here we developed a novel fluorescent reporter, Salmonella INtracellular Analyzer (SINA), compatible for fluorescence microscopy and flow cytometry in single-bacterium level quantification. This identified a S. Typhimurium subpopulation in infected epithelial cells that exhibits a unique phenotype in comparison to the previously documented vacuolar or cytosolic S. Typhimurium. This subpopulation entered a dormant state in a vesicular compartment distinct from the conventional Salmonella-containing vacuoles (SCV) as well as the previously reported niche of dormant S. Typhimurium in macrophages. The dormant S. Typhimurium inside enterocytes were viable and expressed Salmonella Pathogenicity Island 2 (SPI-2) virulence factors at later time points. We found that the formation of these dormant S. Typhimurium is not triggered by the loss of SPI-2 effector secretion but it is regulated by (p)ppGpp-mediated stringent response through RelA and SpoT. We predict that intraepithelial dormant S. Typhimurium represents an important pathogen niche and provides an alternative strategy for S. Typhimurium pathogenicity and its persistence.
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Torres, AnnMarie, Amy Kullas, and Adrianus van der Velden. "Characterization of the mechanism by which L-asparaginase II of Salmonella enterica serovar Typhimurium induces T cell inhibition (99.12)." Journal of Immunology 186, no. 1_Supplement (April 1, 2011): 99.12. http://dx.doi.org/10.4049/jimmunol.186.supp.99.12.

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Abstract T cells play a key role in controlling and clearing infection with the bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium). However, T cell-mediated immunity against S. Typhimurium takes months to develop and has been described as slow and inefficient. Previously, we have shown that S. Typhimurium have a direct inhibitory effect on T cells, down-modulating expression of the beta chain of the T cell receptor (TCR-beta) and inhibiting T cell proliferation. Furthermore, we have shown that a soluble, proteinaceous factor produced or induced by S. Typhimurium is responsible for this inhibition. Most recently, we have found that STM3106 is required for S. Typhimurium to inhibit T cells and that L-asparaginase II, which is encoded by STM3106, is present in the supernatants of T cells cultured in the presence of S. Typhimurium. In addition, we have found that L-asparaginase II produced by S. Typhimurium is both necessary and sufficient for the inhibition of T cells. With further characterization, this research should provide new insight into the mechanism by which S. Typhimurium inhibit T cells, avoiding immune clearance.
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Dissertations / Theses on the topic "Typhimurium"

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Damiani, Igor Alexandre Campos 1988. "Estudo do efeito terapêutico de linhagens atenuadas de Salmonella enterica Typhimurium em modelos murinos de câncer." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317027.

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Orientador: Marcelo Brocchi
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-20T04:43:16Z (GMT). No. of bitstreams: 1 Damiani_IgorAlexandreCampos_M.pdf: 2676549 bytes, checksum: 95c7715f3b322149e80749501a5500a2 (MD5) Previous issue date: 2011
Resumo: Salmonella enterica Typhimurium é uma bactéria anaeróbica facultativa que apresenta tropismo por áreas tumorais. Esta interessante propriedade abre novas perspectivas em relação à pesquisa contra o câncer, pois há muito tempo buscam-se veículos seletivos para a eliminação de neoplasias. A inibição do crescimento tumoral e até mesmo seu total retrocesso foram observados em modelos murinos de câncer tratados com linhagens atenuadas de S. enterica. Além disso, seu potencial como veículo de moléculas antitumorais exógenas (vacina de DNA, RNAi, citocinas e enzimas, por exemplo) também foi descrito. No entanto, as linhagens testadas em humanos até o presente não induziram os mesmos efeitos observados nos modelos animais. Isto indica que estudos adicionais são necessários para otimização desta terapia, incluindo o teste de novas linhagens mutantes atenuadas de S. enterica....Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital
Abstract: Salmonella enterica Typhimurium, a facultative anaerobic bacterium, presents tropism for tumor areas. This interesting property creates new perspectives in cancer research, in which great efforts have been done to seek a drug carrier that could selectively target and destroy malignant cells. Inhibition of tumor growth and even its total elimination were observed in murine cancer models infected by attenuated strains of S. enterica. Besides, its potential as a carrier of exogenous antitumor molecules (DNA vaccine, iRNA, cytokines and enzymes, for example) is also described. Nevertheless, when these strains were tested in humans, they did not induce the same effects observed in murine models. Thus, additional studies are needed to optimize this therapy, including the test of novel S. enterica attenuated strains...Note: The complete abstract is available with the full electronic digital thesis or dissertations
Mestrado
Microbiologia
Mestre em Genética e Biologia Molecular
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Salam, Faridah. "Development of immunosensors for Salmonella typhimurium." Thesis, Cranfield University, 2010. http://dspace.lib.cranfield.ac.uk/handle/1826/8193.

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The accidental contamination of Salmonella in raw and processed foods is a major problem for the food and feed industries worldwide. Rapid detection methods for monitoring and identification are required to solve the health and safety problems related to these pathogenic bacteria. Current detection methods require extensive sample preparation and prolonged assay procedures, thus, this research project focused on developing rapid methods which are capable of sensing these microorganisms at a high sensitivity level.
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de, Almeida Pereira Milton César. "Inflammasome signalling during Salmonella Typhimurium infection." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/283642.

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The innate immune system is the first line of defence against infection. It is comprised of physicochemical barriers and a variety of cell types including macrophages and dendritic cells. Pathogens express specific pathogen associated molecular patterns (PAMP) which are recognised by pattern recognition receptors (PRR) on macrophages to initiate an innate immune response. Gram-negative bacteria such as Salmonella enterica serovar Typhimurium express a range of bacterial PAMPs recognised by Toll-like receptors (TLRs) including lipopolysaccharides (LPS) recognised by TLR-4 and lipoproteins by TLR-2. The activation of TLRs results in activation of nuclear factor κB (NF-κB) to drive transcription of mRNA coding for pro-inflammatory proteins such as tumor necrosis factor α (TNF-α) and pro-interleukin (IL) 1β. Myeloid cells also possess intracellular PRRs including the nucleotide-binding domain and leucine-rich repeat (NLR) family. NLR family CARD domain- containing protein 4 (NLRC4) and NLR family pyrin domain-containing protein 3 (NLRP3) are the main NLRs engaged in recognising S. Typhimurium infection, leading to formation of the inflammasome. The inflammasome is a macromolecular complex assembled in the cytoplasm, and usually contains a NLR, the structural protein apoptosis-associated speck-like protein containing a CARD (ASC) and effector enzymes such as cysteine-dependent aspartate-directed protease (caspase) -1 and caspase-8. This structure is responsible for processing the cytokines pro- IL-1β and pro-IL-18 to their mature form and is involved in triggering a pro-inflammatory process of cell death termed pyroptosis. The formation of the inflammasome therefore results in cell death and secretion of proinflammatory cytokines which play important roles in controlling infections. Inflammasome activity must be tightly coordinated, as its dysregulation is associated with a variety of auto-inflammatory and auto-immune diseases. The signalling events leading to inflammasome assembly are poorly understood and the molecules involved in fine-tuning its activity are only beginning to be discovered. The aim of this thesis was to discover new molecules involved in inflammasome activation and/or in keeping its activity in check. To achieve this goal, I performed S. Typhimurium infection assays in primary bone marrow derived macrophages (BMDM) derived from C57BL/6 mice wild type (WT) and compared the resulting cellular viability, intracellular bacteria counts and IL-1β production to that of BMDMs derived from C57BL/6 mice lacking proteins involved with, or suspected to be involved with, innate immune activity. Amongst the proteins I studied, caspase recruitment domain 9 (CARD9) inhibited inflammasome-mediated IL-1β production. Multiple independent genome-wide association studies link this protein to inflammatory pathologies such as Crohn's disease, but its role in canonical inflammasomes was largely unexplored. To investigate how CARD9 inhibits inflammasome-mediated IL-1β production I have conducted assays in WT and Card9-/- BMDMs, including stimulation of specific NLRs with their purified ligands, infection with bacterial strains deficient in NLRC4 activation, and infection assays in presence of pharmacological inhibitors. By employing these approaches, I observed that CARD9 has a negative role on NLRP3-dependent IL-1β production. Specifically, in response to activation of the NLRP3 by Salmonella infection, CARD9 negatively regulates pro-IL-1β transcription, and decreases IL-1β processing by inhibiting spleen tyrosine kinase (SYK)-mediated NLRP3 activation and represses caspase-8 activity in the inflammasome. CARD9 expression is suppressed in the course of S. Typhimurium infection which may act as a mechanism to increase IL-1β production during the infection. In conclusion, I have established a connection between CARD9 and IL-1β production by the canonical NLRP3 inflammasome and elucidated some of the mechanisms involved in this process. I have also found evidence that other proteins are likely to be involved in inflammasome regulation and the elucidation of their roles will be addressed in future studies.
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Muddiman, Katie. "Functional characterisation of Salmonella Typhimurium CueP." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/functional-characterisation-of-salmonella-typhimurium-cuep(a9ded192-a63d-48f0-a34e-7d9a1ce0e011).html.

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Metals are used as cofactors for enzymes, but are toxic in excess. In order to avoid the deleterious effects posed by metals, the cell must employ strict metal homeostasis systems. One such system is the Cue copper-resistance system in Salmonella enterica serovar Typhimurium (S. Typhimurium) which includes the periplasmic copper binding protein CueP. Previous studies have shown CueP to be a major periplasmic copper-sequestering protein that has a role in supplying copper to, and thus activating, the periplasmic Cu,Zn-superoxide dismutase enzyme SodCII (Osman et al., 2013). SodCII protects the cell from reactive oxygen species (ROS), due for example to the actions of the respiratory burst oxidase in host macrophages. However, despite its ability to sequester copper and activate SodCII, the precise physiological role of CueP in S. Typhimurium has remained unresolved since cueP mutants of S. Typhimurium strain SL1344 (the wild-type stain used in this study) do not exhibit a phenotype with respect to tolerance to copper or reactive oxygen species. In addition, the copper-binding mechanism of CueP and its interactions with other copper-binding proteins, including SodCII, have not been examined. An aim of this study was to establish a phenotype for a cueP mutant of S. Typhimurium with respect to copper and/or ROS tolerance. It was hypothesised that the possession of KatG (catalase) and multiple superoxide dismutases (SodCI, SodA and SodB), in addition to SodCII, by S. Typhimurium may confer functional redundancy with respect to copper and ROS tolerance. Hence mutants lacking katG (ΔkatG) or the various superoxide dismutase encoding genes (ΔsodA/ΔsodB/ΔsodCI/ΔsodCII) with and without functional cueP were generated. The ΔkatG mutants exhibited reduced catalase activity and reduced tolerance to hydrogen peroxide, consistent with the loss of KatG, however the additional loss of cueP did not reduce tolerance to hydrogen peroxide further. Similarly, tolerance to copper and extracellular superoxide was also unaltered in the ΔkatG/ΔcueP mutant. The tolerance of the various superoxide dismutase mutants to copper and various ROS was also unaffected by the presence or absence of CueP. To examine the role of CueP in SodCII activation in vivo, SodCII was over-expressed in S. Typhimurium (in a ΔsodA/ΔsodB/ΔsodCI/ΔsodCII background) with and without functional cueP and superoxide dismutase activity measured in both whole cells and periplasmic extracts. SodCII-dependent superoxide dismutase activity was successfully identified within the periplasmic extracts. However, surprisingly, the level of activity was unaffected by the presence 16 or absence of CueP and/or the addition of copper. It is possible that SodCII is thus able to scavenge sufficient copper for activity from the reagents used in these assays. Similarly, in an alternative approach to examine the role of CueP in vitro, both SodCII and CueP (WT and potential metal-binding residue mutant forms) were successfully over-expressed in E. coli and methods for their purification optimised (without the use of affinity tags). ICP-MS analysis indicated that a CuePC104S mutant contains > 18-fold less copper than the CueP WT protein. Furthermore, superoxide dismutase activity assays using purified proteins, indicated that the CuePC104S mutant was less able to activate SodCII than the WT CueP. Taken together, these results are consistent with a role for the Cys104 residue in copper-binding by CueP. Bioinformatics results suggest the presence of CueP or homologous genes in the presence of other bacteria, including pathogens such as Klebsiella, Yersinia and Shigella spp. Further understanding of the role of CueP and the systems used by S. Typhimurium to avoid both copper and ROS stress may inform the development of novel treatment strategies for bacterial diseases.
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Wang, Liying. "Regulation of heme biosynthesis targets the key enzyme HemA by a mechanism of protein stabilization in Salmonella typhimurium." Morgantown, W. Va. : [West Virginia University Libraries], 1999. http://etd.wvu.edu/templates/showETD.cfm?recnum=577.

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Thesis (Ph. D.)--West Virginia University, 1999.
Title from document title page. Document formatted into pages; contains xiii, 145 p. : ill. (some col.) Includes abstract. Includes bibliographical references.
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Bergman, Molly Ann. "Host responses to Salmonella typhimurium infection in vitro and in vivo /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/11503.

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Brahmbhatt, Himanshu N. "Cloning and molecular characterization of the rfb gene cluster from Salmonella typhimurium LT2 /." Title page, contents and abstract only, 1987. http://web4.library.adelaide.edu.au/theses/09PH/09phb813.pdf.

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Tai, Chia-Hui. "Kinetic and Chemical Mechanism of O-Acetylserine Sulfhydrylase-B from Salmonella Typhimurium." Thesis, University of North Texas, 1993. https://digital.library.unt.edu/ark:/67531/metadc279064/.

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Initial velocity studies of O-acetylserine sulfhydrylase-B (OASS-B) from Salmonella typhimurium using both natural and alternative substrates suggest a Bi Bi ping pong kinetic mechanism with double substrate competitive inhibition. The ping pong mechanism is corroborated by a qualitative and quantitative analysis of product and dead-end inhibition. Product inhibition by acetate is S-parabolic noncompetitive, indication of a combination of acetate with E followed by OAS. These data suggest some randomness to the OASS-B kinetic mechanism. The pH dependence of kinetic parameters was determined in order to obtain information on the acid-base chemical mechanism for the OASS-B reaction. A mechanism is proposed in which an enzyme general base accepts a proton from α-amine of O-acetylserine, while a second enzyme general base acts by polarizing the acetyl carbonyl assisting in the β-elimination of the acetyl group of O-acetylserine. The ε-amine of the active site lysine acts as a general base to abstract the α-proton in the β-elimination of acetate. At the end of the first half reaction the ε-amine of the active site lysine that formed the internal Schiff base and the general base are protonated. The resulting α-aminoacrylate intermediate undergoes a Michael addition with HS‾ and the active site lysine donates its proton to the α-carbon to give cysteine and regenerate enzyme to start the second half reaction. In addition, substrate specificity, stereochemistry of the internal Schiff base at C4', and sequence around active site lysine of O-acetylserine sulfhydrylase-A have been determined. The [4'-^3H]pyridoxamine generated by reduction of the internal Schiff base with sodium [^3H]borohydride retained most of its tritium after incubation with apoaspartate aminotransferase. These results agree with the hypothesis put forth by Dunathan (Dunathan, 1971; Dunathan and Voet, 1974) that a single surface (Re face) of the active site PLP is accessible to solvent. The sequence around the active site lysine is AsnProSerPheSerValLysCysArg.
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Hone, David. "Construction of Salmonella vaccines /." Title page, contents and abstract only, 1988. http://web4.library.adelaide.edu.au/theses/09PH/09phh7721.pdf.

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Müller, Andreas Johann. "In vivo analysis of Salmonella typhimurium infection /." [S.l.] : [s.n.], 2009. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=18432.

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Books on the topic "Typhimurium"

1

Lemoine, V. Rodríguez. Herencia extracromosómica en Salmonella typhimurium. Caracas: Universidad Central de Venezuela, Consejo de Desarrollo Científico y Humanístico, 1991.

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Dahlfors, Agneta A. Richter. Regulation of the cobalamin biosynthetic genes in Salmonella typhimurium. Uppsala: Acta Universitatis Upsaliensis, 1994.

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Morgan, Eirwen. Direct search for salmonella serotype Typhimurium gut invasins. Birmingham: University of Birmingham, 2000.

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1931-, Neidhardt Frederick C., ed. Escherichia coli and Salmonella typhimurium: Cellular and molecular biology. Washington, D.C: American Society for Microbiology, 1987.

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Franchet, Nicola. Physiological fitness and survival behaviour of Salmonella Typhimurium DT104. Birmingham: University of Birmingham, 2000.

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Worton, Kim Julie. Studies on the association of Salmonella typhimurium with intestinal mucosae. Birmingham: University of Birmingham, 1988.

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Smith, Bradley Michael. Fate of Salmonella typhimurium and total coliforms during bacterial leaching. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1992.

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Robey, Marianne. Analysis of salmonella typhimurium invasion-complemented recombinants with respect to gastroenteritis. Birmingham: University of Birmingham, 1996.

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Dumitru, Iona. Construction of a Salmonella typhimurium strain expressing the Camplylobactor flaA gene. Ottawa: National Library of Canada, 1994.

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Wallis, T. S. Experimental studies on the induction of fluid secretion by Salmonella typhimurium. Birmingham: University of Birmingham, 1987.

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Book chapters on the topic "Typhimurium"

1

Liu, Shu-Lin, Andrew Hessel, Michael McClelland, and Kenneth E. Sanderson. "Salmonella typhimurium." In Bacterial Genomes, 737–39. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_78.

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Eynard, Natalie. "Electrotransformation of Salmonella typhimurium." In Electrotransformation of Bacteria, 66–71. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-662-04305-9_7.

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Bäumler, A. J., R. M. Tsolis, and F. Heffron. "Fimbrial Adhesins of Salmonella Typhimurium." In Advances in Experimental Medicine and Biology, 149–58. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4899-1828-4_23.

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Rhen, Mikael, Suvi Taria, Soila Sukupolvi, Marita Virtanen, and P. Helena Makela. "The Salmonella Typhimurium Virulence Plasmid." In Microbial Surface Components and Toxins in Relation to Pathogenesis, 107–14. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-8995-8_13.

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Cooper, Stephen, David Gally, Yuko Suneoka, Melissa Penwell, Kelly Caldwell, and Kelvin Bray. "Peptidoglycan Synthesis in Salmonella Typhimurium." In Bacterial Growth and Lysis, 161–68. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9359-8_18.

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Forster, R., and R. D. Gwilliam. "Reverse Mutation in Salmonella typhimurium." In Comparative Genetic Toxicology, 55–58. London: Palgrave Macmillan UK, 1985. http://dx.doi.org/10.1007/978-1-349-07901-8_6.

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Stephen, John, Iqbal I. Amin, and Gillian R. Douce. "Experimental Salmonella typhimurium — Induced Gastroenteritis." In Biology of Salmonella, 199–209. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2854-8_23.

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Rabsch, Wolfgang. "Salmonella Typhimurium Phage Typing for Pathogens." In Methods in Molecular Biology, 177–211. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-512-1_10.

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Kredich, Nicholas M., M. Danuta Hulanicka, and Scott G. Hallquist. "Synthesis of L-Cysteine inSalmonella typhimurium." In Ciba Foundation Symposium 72 - Sulphur in Biology, 87–99. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470720554.ch6.

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Nagasawa, T., T. Satoda, K. Tanizawa, and H. Yamada. "Diaminopropionate Ammonia-Lyase Of Salmonella Typhimurium." In Biochemistry of Vitamin B6, 229–32. Basel: Birkhäuser Basel, 1987. http://dx.doi.org/10.1007/978-3-0348-9308-4_42.

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Conference papers on the topic "Typhimurium"

1

Dahl, J., A. Wingstrand, D. L. Baggesen, and Bent Nielsen. "Strategies for elimination of S. typhimurium." In Seventh International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 1997. http://dx.doi.org/10.31274/safepork-180809-198.

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Jordan, D., T. Kaiser, and G. Cline. "Efficacy of a Salmonella Typhimurium and Salmonella Choleraesuis experimental combination vaccine against S. Typhimurium challenge in growing pigs." In 10th International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork. Iowa State University, Digital Press, 2013. http://dx.doi.org/10.31274/safepork-180809-921.

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Mulero, Rafael, William R. Hesse, Liang Wu, and Min Jun Kim. "Probing Bacterial Flagellar Polymorphism in Various Fluidic Environments Using Solid-State Sub-Micropores." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-66670.

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A novel method for the detection of an assortment of environmental conditions in a microfluidic system using bacterial flagella and submicro-scale solid state pores is presented. Differences in various environmental conditions stimulate the polymorphic helix structure of Salmonella typhimurium flagella to transform to its lowest energetic conformation. By measuring the ionic current blockage (resistive pulse) as flagella electrophoretically translocate a submicro-scale pore, detection of the polymorphic state of flagella corresponding to the conditions of the environmental stimuli is possible. We test the viability of this method using purified depolymerized and repolymerized S. Typhimurium flagella and a high resolution electrical signal readout sub-micropore-based detection system.
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Hesse, William R., Matthew Federici, David M. Casale, Peter Fink, Basil Milton, and Min Jun Kim. "Biologically Inspired Robotic Microswimmers." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-10565.

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Drug delivery systems have had a profound impact on several branches of medicine. Engineers and researchers alike have labored to create a controlled drug delivery device capable of regulated dosage release and a specific cell targeting mechanism. The growing field of biomimicry has inspired several of these drug systems, though success has been limited. The flagellated low Reynolds number propulsion system of Salmonella typhimurium has inspired this specific delivery complex. In this system, the helical flagellar filaments of S. typhimurium are isolated from the bacteria’s cell body and are bound to functionalized paramagnetic microspheres. As a magnetic field is applied to this device, the microsphere rotates, inducing rotation of the helical flagella. This motion creates a locomotive force and drives the device in a predestined direction.
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Waiwijit, Uraiwan, Kata Jaruwongrungsee, Nipa Chokesajjawatee, Jurairat Promjai, Tanom Lomas, Pornpimol Sritongkham, and Adisorn Tuantranont. "QCM-Based DNA biosensor for Salmonella typhimurium detection." In 2012 IEEE International Conference of Electron Devices and Solid-State Circuits (EDSSC). IEEE, 2012. http://dx.doi.org/10.1109/edssc.2012.6482844.

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Gao, Xuefeng, Sabin Tabirca, and Mark Tangney. "Computer simulation of Salmonella typhimurium accumulation within tumors." In the 9th International Conference. New York, New York, USA: ACM Press, 2011. http://dx.doi.org/10.1145/2037509.2037526.

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Anderson, Timoty J., Toni L. Poole, Robin C. Anderson, and David J. Nisbet. "Persistence of Salmonella typhimurium in porcine gut microflora." In Sixth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2005. http://dx.doi.org/10.31274/safepork-180809-782.

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Tay, Li-Lin, Ping-Ji Huang, and Lai-Kwan Chau. "Plasmonic Sensors for Pathogen Detection." In Applied Industrial Spectroscopy. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/ais.2022.jm2e.5.

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Plasmonic surface enhanced Raman scattering (SERS) probes were synthesized with different Raman reporter molecules and conjugated with pathogen specific antibodies to provide a rapid screening platform for the detection of pathogenic bacterium, S. aureus and S. Typhimurium.
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Isaacson, Richard E. "Research on Persistent Colonization of Pigs by Salmonella typhimurium and the Effects of Transportation Related Stress on Shedding of Salmonella typhimurium." In Seventh International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 1996. http://dx.doi.org/10.31274/safepork-180809-150.

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Kim, Won Il, Byoung Yeal Jung, Kyu Tae Kim, Kwang Hyun Cho, Ryun Bin Tak, and Bong Hwan Kim. "Detection of multiresistant Salmonella typhimurium DT104 by multiplex PCR." In Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2001. http://dx.doi.org/10.31274/safepork-180809-1195.

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Reports on the topic "Typhimurium"

1

Olsen, Eric V., Iryna B. Sorokulova, I.-Hsuan Chen, Ben Fiebor, and James M. Barbaree. Landscape Phage Probes for Salmonella Typhimurium. Fort Belvoir, VA: Defense Technical Information Center, January 2004. http://dx.doi.org/10.21236/ada426603.

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Andrews, Paul W., Raymond R. Tice, and Diane Satterfield. Salmonella Typhimurium Microsome Reverse Mutation Assay. Fort Belvoir, VA: Defense Technical Information Center, March 1996. http://dx.doi.org/10.21236/ada589278.

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Amaya Gómez, Carol Viviana, Liz Alejandra Uribe Gutiérrez, Sabrina del Carmen Jiménez Velásquez, Geraldine Tibasosa Rodriguez, and Deisy Lisseth Toloza Moreno. Actividad antagónica de la colección de mohos del banco de germoplasma de AGROSAVIA frente a bacterias enteropatogénicas resistentes a antibióticos. Corporación colombiana de investigación agropecuaria - AGROSAVIA, 2018. http://dx.doi.org/10.21930/agrosavia.poster.2018.3.

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La colección de mohos del Banco de Germoplasma de Microorganismos de AGROSAVIA (BGMA) tiene actividad biocontroladora contra insectos plaga que afectan cultivos comerciales, sin embargo, existe un limitado conocimiento acerca de su potencial para controlar el crecimiento de enterobacterias como Escherichia coli O157 y Salmonella typhimurium presentes en alimentos contaminados. En este estudio se inició la caracterización de la actividad antagónica de 100 mohos de 25 géneros diferentes, aislados en 16 departamentos de Colombia. Para determinar la actividad antagonista, los mohos y los patógenos se crecieron en medio de ciente en hierro (IDM) y medio R2A. La cepa Mt008 presentó mayor actividad antagónica frente a E. coli O157 en R2A y S. typhimurium en IDM. Mt009 y Nm010 presentaron mayor actividad antagónica frente a E. coli O157 y S. typhimurium en IDM y menor actividad en R2A. Los resultados sugieren que la actividad antagónica exhibida por los mohos podría estar infuenciada por la composición del medio y la presencia del patógeno.
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Boury, Nancy M., N. K. Lee, and D. L. Hank Harris. Use of bacteriophage Felix01, HL18 and HL03 to reduce Salmonella enterica Typhimurium burden in mice. Ames (Iowa): Iowa State University, January 2005. http://dx.doi.org/10.31274/ans_air-180814-1089.

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Michaelsen, A. R., and Joseph G. Sebranek. Microbial Inhibitors Combined with Modified Atmosphere Packaging for Improved Control of Salmonella Typhimurium on Pork Products. Ames (Iowa): Iowa State University, January 2005. http://dx.doi.org/10.31274/ans_air-180814-1117.

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Kudra, Li, Joseph G. Sebranek, James S. Dickson, Aubrey F. Mendonca, Armitra Jackson-Davis, Qijing Zhang, Kenneth J. Prusa, and Zheng Lu. Controlling Listeria monocytogenes, Campylobactor jejuni, Salmonella enterica Typhimurium and Escherichia coli O157:H7 in Meat Products by Irradiation Combined with Modified Atmosphere Packaging. Ames (Iowa): Iowa State University, January 2013. http://dx.doi.org/10.31274/ans_air-180814-13.

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Song, Jian, and Paul E. Kirby. Evaluation of a Test Article in the Salmonella typhimurium/Ecscherichia coli Plate Incorporation Mutation Assay in the Presence and Absence of Induced Rat Liver S-9. Test Article: Ethylenediamine Dinitrate (EDDN). Fort Belvoir, VA: Defense Technical Information Center, February 2009. http://dx.doi.org/10.21236/ada518253.

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Song, Jian. Evaluation of a Test Article in the Salmonella typhimurium/Escherichia coli Plate Incorporation Mutation Assay in the Presence and Absence of Induced Rat Liver S-9. Test Article 3-Nitro-1,2,4-Triazol-5-one (NTO). Fort Belvoir, VA: Defense Technical Information Center, October 2008. http://dx.doi.org/10.21236/ada518833.

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Kirby, Paul E. Evaluation of a Test Article in the Salmonella typhimurium/Escherichia coli Plate Incorporation Mutation Assay in the Presence and Absence of Induced Rat Liver S-9. Test Article: N,N,N',N'-tetramethyl ethanediamine (TMEDA). Fort Belvoir, VA: Defense Technical Information Center, June 2008. http://dx.doi.org/10.21236/ada519620.

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Irudayaraj, Joseph, Ze'ev Schmilovitch, Amos Mizrach, Giora Kritzman, and Chitrita DebRoy. Rapid detection of food borne pathogens and non-pathogens in fresh produce using FT-IRS and raman spectroscopy. United States Department of Agriculture, October 2004. http://dx.doi.org/10.32747/2004.7587221.bard.

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Rapid detection of pathogens and hazardous elements in fresh fruits and vegetables after harvest requires the use of advanced sensor technology at each step in the farm-to-consumer or farm-to-processing sequence. Fourier-transform infrared (FTIR) spectroscopy and the complementary Raman spectroscopy, an advanced optical technique based on light scattering will be investigated for rapid and on-site assessment of produce safety. Paving the way toward the development of this innovative methodology, specific original objectives were to (1) identify and distinguish different serotypes of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, and Bacillus cereus by FTIR and Raman spectroscopy, (2) develop spectroscopic fingerprint patterns and detection methodology for fungi such as Aspergillus, Rhizopus, Fusarium, and Penicillium (3) to validate a universal spectroscopic procedure to detect foodborne pathogens and non-pathogens in food systems. The original objectives proposed were very ambitious hence modifications were necessary to fit with the funding. Elaborate experiments were conducted for sensitivity, additionally, testing a wide range of pathogens (more than selected list proposed) was also necessary to demonstrate the robustness of the instruments, most crucially, algorithms for differentiating a specific organism of interest in mixed cultures was conceptualized and validated, and finally neural network and chemometric models were tested on a variety of applications. Food systems tested were apple juice and buffer systems. Pathogens tested include Enterococcus faecium, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus, Yersinia enterocolitis, Shigella boydii, Staphylococus aureus, Serratiamarcescens, Pseudomonas vulgaris, Vibrio cholerae, Hafniaalvei, Enterobacter cloacae, Enterobacter aerogenes, E. coli (O103, O55, O121, O30 and O26), Aspergillus niger (NRRL 326) and Fusarium verticilliodes (NRRL 13586), Saccharomyces cerevisiae (ATCC 24859), Lactobacillus casei (ATCC 11443), Erwinia carotovora pv. carotovora and Clavibacter michiganense. Sensitivity of the FTIR detection was 103CFU/ml and a clear differentiation was obtained between the different organisms both at the species as well as at the strain level for the tested pathogens. A very crucial step in the direction of analyzing mixed cultures was taken. The vector based algorithm was able to identify a target pathogen of interest in a mixture of up to three organisms. Efforts will be made to extend this to 10-12 key pathogens. The experience gained was very helpful in laying the foundations for extracting the true fingerprint of a specific pathogen irrespective of the background substrate. This is very crucial especially when experimenting with solid samples as well as complex food matrices. Spectroscopic techniques, especially FTIR and Raman methods are being pursued by agencies such as DARPA and Department of Defense to combat homeland security. Through the BARD US-3296-02 feasibility grant, the foundations for detection, sample handling, and the needed algorithms and models were developed. Successive efforts will be made in transferring the methodology to fruit surfaces and to other complex food matrices which can be accomplished with creative sampling methods and experimentation. Even a marginal success in this direction will result in a very significant breakthrough because FTIR and Raman methods, in spite of their limitations are still one of most rapid and nondestructive methods available. Continued interest and efforts in improving the components as well as the refinement of the procedures is bound to result in a significant breakthrough in sensor technology for food safety and biosecurity.
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