Academic literature on the topic 'Type IV filaments'

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Journal articles on the topic "Type IV filaments"

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Ching, GY, and RK Liem. "Assembly of type IV neuronal intermediate filaments in nonneuronal cells in the absence of preexisting cytoplasmic intermediate filaments." Journal of Cell Biology 122, no. 6 (1993): 1323–35. http://dx.doi.org/10.1083/jcb.122.6.1323.

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We report here on the in vivo assembly of alpha-internexin, a type IV neuronal intermediate filament protein, in transfected cultured cells, comparing its assembly properties with those of the neurofilament triplet proteins (NF-L, NF-M, and NF-H). Like the neurofilament triplet proteins, alpha-internexin coassembles with vimentin into filaments. To study the assembly characteristics of these proteins in the absence of a preexisting filament network, transient transfection experiments were performed with a non-neuronal cell line lacking cytoplasmic intermediate filaments. The results showed tha
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Couturier, Marc Roger, and Markus Stein. "Helicobacter pylori produces unique filaments upon host contact in vitro." Canadian Journal of Microbiology 54, no. 7 (2008): 537–48. http://dx.doi.org/10.1139/w08-042.

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Helicobacter pylori exists in 2 distinct morphological states, helicoid and coccoid. Both have been observed in in vitro culture and in gastric biopsies. We visualized H. pylori during AGS cell infections using immunofluorescence microscopy. Anti-H. pylori mouse serum as well as human serum from H. pylori-positive patients recognized long, thin bacterial filaments, which formed on helicoids and more frequently on coccoids. These filaments reached lengths of 59 μm and often connected bacteria. Periodate oxidation abolished antibody recognition, suggesting that carbohydrates compose a major anti
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Goosens, Vivianne J., Andreas Busch, Michaella Georgiadou, et al. "Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili." Proceedings of the National Academy of Sciences 114, no. 25 (2017): E4978—E4986. http://dx.doi.org/10.1073/pnas.1618539114.

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Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heterologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes a
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Kraljic, Katarina, Christopher Duckworth, Rita Tojeiro, et al. "SDSS-IV MaNGA: 3D spin alignment of spiral and S0 galaxies." Monthly Notices of the Royal Astronomical Society 504, no. 3 (2021): 4626–33. http://dx.doi.org/10.1093/mnras/stab1109.

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ABSTRACT We investigate the 3D spin alignment of galaxies with respect to the large-scale filaments using the MaNGA survey. The cosmic web is reconstructed from the Sloan Digital Sky Survey using disperse and the 3D spins of MaNGA galaxies are estimated using the thin disc approximation with integral field spectroscopy kinematics. Late-type spiral galaxies are found to have their spins parallel to the closest filament’s axis. The alignment signal is found to be dominated by low-mass spirals. Spins of S0-type galaxies tend to be oriented preferentially in perpendicular direction with respect to
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Braun, Tatjana, Matthijn R. Vos, Nir Kalisman, et al. "Archaeal flagellin combines a bacterial type IV pilin domain with an Ig-like domain." Proceedings of the National Academy of Sciences 113, no. 37 (2016): 10352–57. http://dx.doi.org/10.1073/pnas.1607756113.

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The bacterial flagellar apparatus, which involves ∼40 different proteins, has been a model system for understanding motility and chemotaxis. The bacterial flagellar filament, largely composed of a single protein, flagellin, has been a model for understanding protein assembly. This system has no homology to the eukaryotic flagellum, in which the filament alone, composed of a microtubule-based axoneme, contains more than 400 different proteins. The archaeal flagellar system is simpler still, in some cases having ∼13 different proteins with a single flagellar filament protein. The archaeal flagel
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Sheppard, Devon, Jamie-Lee Berry, Rémi Denise, Eduardo P. C. Rocha, Steve Matthews, and Vladimir Pelicic. "The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold." Journal of Biological Chemistry 295, no. 19 (2020): 6594–604. http://dx.doi.org/10.1074/jbc.ra120.013316.

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Type IV filaments (T4F), which are helical assemblies of type IV pilins, constitute a superfamily of filamentous nanomachines virtually ubiquitous in prokaryotes that mediate a wide variety of functions. The competence (Com) pilus is a widespread T4F, mediating DNA uptake (the first step in natural transformation) in bacteria with one membrane (monoderms), an important mechanism of horizontal gene transfer. Here, we report the results of genomic, phylogenetic, and structural analyses of ComGC, the major pilin subunit of Com pili. By performing a global comparative analysis, we show that Com pi
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Daehnel, Katrin, Robin Harris, Lucinda Maddera, and Philip Silverman. "Fluorescence assays for F-pili and their application." Microbiology 151, no. 11 (2005): 3541–48. http://dx.doi.org/10.1099/mic.0.28159-0.

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Conjugative pili are extracellular filaments elaborated by Gram-negative bacteria expressing certain type IV secretion systems. They are required at the earliest stages of conjugal DNA transfer to establish specific and secure cell–cell contacts. Conjugative pili also serve as adsorption organelles for both RNA and DNA bacteriophages. Beyond these facts, the structure, formation and function of these filaments are poorly understood. This paper describes a rapid, quantitative assay for F-pili encoded by the F plasmid type IV secretion system. The assay is based on the specific lateral adsorptio
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Ching, G. Y., and R. K. Liem. "Analysis of the roles of the head domains of type IV rat neuronal intermediate filament proteins in filament assembly using domain-swapped chimeric proteins." Journal of Cell Science 112, no. 13 (1999): 2233–40. http://dx.doi.org/10.1242/jcs.112.13.2233.

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Type IV neuronal intermediate filament proteins consist of alpha-internexin, which can self-assemble into filaments and the neurofilament triplet proteins, which are obligate heteropolymers, at least in rodents. These IF proteins therefore provide good systems for elucidating the mechanism of intermediate filament assembly. To analyze the roles of the head domains of these proteins in contributing to their differential assembly properties, we generated chimeric proteins by swapping the head domains between rat alpha-internexin and either rat NF-L or NF-M and examined their assembly properties
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Lamparter, Tilman, Jennifer Babian, Katrin Fröhlich, et al. "The involvement of type IV pili and the phytochrome CphA in gliding motility, lateral motility and photophobotaxis of the cyanobacterium Phormidium lacuna." PLOS ONE 17, no. 1 (2022): e0249509. http://dx.doi.org/10.1371/journal.pone.0249509.

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Phormidium lacuna is a naturally competent, filamentous cyanobacterium that belongs to the order Oscillatoriales. The filaments are motile on agar and other surfaces and display rapid lateral movements in liquid culture. Furthermore, they exhibit a photophobotactic response, a phototactic response towards light that is projected vertically onto the area covered by the culture. However, the molecular mechanisms underlying these phenomena are unclear. We performed the first molecular studies on the motility of an Oscillatoriales member. We generated mutants in which a kanamycin resistance casset
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Steinert, Peter M., Lyuben N. Marekov та David A. D. Parry. "Molecular Parameters of Type IV α-Internexin and Type IV-Type III α-Internexin-Vimentin Copolymer Intermediate Filaments". Journal of Biological Chemistry 274, № 3 (1999): 1657–66. http://dx.doi.org/10.1074/jbc.274.3.1657.

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Dissertations / Theses on the topic "Type IV filaments"

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Jacobsen, Theis. "Structure and assembly of bacterial type IV filaments unravelled by an integrative approach." Electronic Thesis or Diss., Sorbonne université, 2022. http://www.theses.fr/2022SORUS146.

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La superfamille des filaments de type IV (TFF) est un groupe de machines moléculaires localisées dans la membrane des bactéries et des archées. Ces machines associent des polymères protéiques de manière non-covalente appelés des pili, qui s’étendent depuis la cellule pour réaliser plusieurs fonctions qui ont évolué spécifiquement pour s’adapter à des organismes hôtes différents. La superfamille TFF comprend le système de sécrétion de type II (T2SS) et les pili de type IVa (T4aP). Le T2SS induit la sécrétion de substrats chez les bactéries Gram-négatives. Ces substrats sont en général des enzym
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Leh, David. "Optimisation du dimensionnement d'un réservoir composite type IV pour stockage très haute pression d'hydrogène." Phd thesis, Université de Grenoble, 2013. http://tel.archives-ouvertes.fr/tel-00942731.

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Ce travail a pour but de proposer une nouvelle approche du dimensionnement optimisé des réservoirs de stockage d'hydrogène de type IV visant à mieux répondre aux enjeux industriels. Les objectifs scientifiques et techniques consistent à disposer de modèles qualifiés pour la simulation du comportement de ces réservoirs, associés à des méthodologies de dimensionnement et d'optimisation fiables. La démarche s'appuie sur trois axes principaux :- proposer une démarche de conception prédictive en intégrant (i) un premier aspect lié à la ruine de la structure qui est la conséquence de mécanismes comp
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Mouville, Clémence. "Interaction entre les pili de type IV et le phage filamenteux MDA : impact potentiel sur la virulence de Neisseria meningitidis." Electronic Thesis or Diss., Université Paris Cité, 2024. http://www.theses.fr/2024UNIP5235.

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Neisseria meningitidis (Nm) est une bactérie commensale du nasopharynx humain qui traverse quelques fois la barrière nasopharyngée et se propage dans la circulation sanguine jusqu'à atteindre les méninges. Un bactériophage filamenteux appelé MDA (Meningococcal Disease Associated) est associé aux infections invasives à méningocoques chez les jeunes adultes. Le MDA semble augmenter l'incidence de la maladie en augmentant la colonisation bactérienne au point d'entrée. L'objectif de ce travail a été de comprendre le mécanisme moléculaire précis de l'infection de Nm par le MDA. L'étude des mutants
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Book chapters on the topic "Type IV filaments"

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Weber, Klaus. "Evolutionary aspects of IF proteins." In Guidebook to the Cytoskeletal and Motor Proteins. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780198599579.003.0094.

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Abstract Extensive work on cytoplasmic intermediate filament (IF) proteins of vertebrates has established a very complex multigene family with close to 50 members in a mammal. These proteins include IF types I to IV as well as additional species. Variability arises primarily from the N- and C-terminal head and tail domains but also from the central α-helical rod domain, which, except for its ends, preserves sequence principles rather than actual sequences. In spite of such differences the helix 1B subdomain keeps a constant length in all cytoplasmic IF proteins so far characterized from verteb
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Conference papers on the topic "Type IV filaments"

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Farhood, Naseer H., Saravanan Karuppanan, H. H. Ya, and Mohamad Ariff Baharom. "Burst pressure investigation of filament wound type IV composite pressure vessel." In ADVANCED MATERIALS FOR SUSTAINABILITY AND GROWTH: Proceedings of the 3rd Advanced Materials Conference 2016 (3rd AMC 2016). Author(s), 2017. http://dx.doi.org/10.1063/1.5010482.

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BIANCHI, I. "Process and structural simulation for the development of a pressure vessel through filament winding technology." In Material Forming. Materials Research Forum LLC, 2023. http://dx.doi.org/10.21741/9781644902479-38.

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Abstract. Recently, Università Politecnica delle Marche and COMEC Innovative srl are involved in the research project “Smart Tow Winding” funded by MIUR (Ministry of Education, University and Research), concerning the development of an innovative process for the realization of a pressure vessel through Filament Winding (FW) technology. In this context, a design procedure for type IV composite pressure vessel is proposed. To design the component, the dedicated simulation software CADWIND was used to virtually generate the pressure vessel through the definition of the desired geometry, the type
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Reports on the topic "Type IV filaments"

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Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate
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