Dissertations / Theses on the topic 'Type III secretion helper proteins'
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Demirjian, Choghag. "Deciphering Arabidopsis thaliana responses to Ralstonia solanacearum virulence factors through the study of plant natural variation." Thesis, Toulouse 3, 2022. http://www.theses.fr/2022TOU30109.
Full textRalstonia solanacearum, the causal agent of bacterial wilt, is considered one of the world’s most important bacterial pathogens. This soil-borne bacterium relies mainly on its type III secretion system (T3SS) and type III effectors (T3Es) in order to cause disease in more than 250 plant species. R. solanacearum injects its T3Es through this molecular syringe directly inside the host plant. These T3Es hijack plant defense responses in either the cytoplasm or the nucleus aiming to suppress plant immunity and promote bacterial multiplication. T3E secretion is finely controlled at the post-translational level by helper proteins, called T3SS control proteins, and type III chaperones.To date, the in planta function of these effectors and helper proteins and how R. solanacearum modulates plant genes to its favor remains poorly understood. My thesis project aimed to better understand the role of R. solanacearum pathogenicity determinants by identifying some of the direct or indirect plant targets of A. thaliana, modulated by the bacterium. For this purpose, I used natural populations of A. thaliana on two geographical scales and adopted the approach of challenging mapping populations to R. solanacearum mutants in which major pathogenic determinants are mutated. This approach is new since most of the GWAS (Genome-Wide Association Studies) in plant-pathogen interactions use wild-type strains of phytopathogens. Furthermore, it unveiled a previously undetected diversity of responses. In the first part of my Ph.D. project, I identified QTLs (Quantitative Trait Loci) involved in quantitative disease resistance to R. solanacearum single mutants and I validated these QTLs as susceptibility factors. In the second part of my thesis, we studied a gene encoding for a NLR protein that we called Bacterial Wilt Susceptibility 1 (BWS1). We showed that BWS1 was acting as quantitative susceptibility factor, mediating a negative regulation of an SGT1-dependent immune response
Bailey, Christopher Michael. "A Bioinformatics Analysis of Bacterial Type-III Secretion System Genes and Proteins." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/1300/.
Full textPanina, Ekaterina Mikhailovna. "Identification and characterization of type III secretion effector proteins in gram-negative bacteria." Diss., Restricted to subscribing institutions, 2007. http://proquest.umi.com/pqdweb?did=1481675641&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.
Full textMuschiol, Sandra. "Small molecule inhibitors of type III secretion and their effect on Chlamydia development." Stockholm, 2009. http://diss.kib.ki.se/2009/978-91-7409-645-3/.
Full textSmollett, Katherine Louise. "Characterisation of the enteropathogenic E. coli type III secretion system effector proteins ESPG and ESPG2." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439818.
Full textHaraga, Andrea. "Study of the intracellular function of the Salmonella enterica serovar Typhimurium type III secretion effector SspH1 /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/11486.
Full textOhlson, Maikke B. "Characterization of the intracellular activities of SseJ and SifA, two Salmonella enterica serovar typhimurium type III secretion effector proteins /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/11485.
Full textWilson, Rebecca Kerry. "Functional analysis of EscF and EscJ : two structural proteins of the type III secretion system of enteropathogenic Escherichia coli." Thesis, Imperial College London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406326.
Full textAlzahrani, Ashwag. "Identification of Human Proteins Interacting with the Protein IcsB of Shigella flexneri." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38333.
Full textEdqvist, Petra J. "Multiple twists in the molecular tales of YopD and LcrH in type III secretion by Yersinia pseudotuberculosis." Doctoral thesis, Umeå : Umeå University, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-985.
Full textVromman, Francois. "The intracellular pathogen Chlamydia trachomatis targets proteins of the ESCRT machinery." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066163/document.
Full textChlamydia trachomatis is an obligate intracellular human pathogen. It is the first infectious cause of blindness and the most common cause of sexually transmitted diseases of bacterial origin. Using a strain of C. trachomatis serovar L2 expressing a fluorescent protein we developed microscopy and flow cytometry based methods to quantify several steps of its developmental cycle. These methods will facilitate future studies aimed at testing anti-bacterial compounds or various culture conditions. Chlamydiae interfere with many cellular processes, in particular via the secretion of bacterial proteins through a type 3 secretion (T3S) system. We identified a family of proteins that possess T3S signals. They share a domain designated as DUF582, which is only found in pathogenic chlamydiae. We showed that the five DUF582 proteins of C. trachomatis are expressed from the mid phase of infection. We demonstrated that the protein Hrs is a common interactor for the DUF582. In addition the N-terminal part of the DUF582 protein CT619 interacts with Tsg101. Hrs and Tsg101 are both important components of the ESCRT machinery, which is an ancient machinery required for several processes involving membrane fission.Using RNA interference we showed that Hrs and Tsg101 are dispensable for bacterial entry and growth. This last result suggest that DUF582 proteins actually prevent Hrs and/or Tsg101 driven processes. Alternatively, the bacteria might highjack the ESCRT machinery but redundant mechanisms would explain the absence of phenotype on bacterial development observed in the silencing experiments. We discuss three hypotheses as to the possible role of the DUF582 proteins in infection
Arroyo, Velez Noe. "Effets des effecteurs de type III de Xanthomonas campestris pv campestris dans la physiologie d'Arabidopsis." Thesis, Toulouse 3, 2022. http://www.theses.fr/2022TOU30064.
Full textXanthomonas campestris pv. campestris (Xcc) causes black rot disease on Brassicaceae species including cabbages, radish, mustard and the model species Arabidopsis thaliana. During pathogenesis, Xcc secrete Type 3 Effector (T3E) proteins via the Type 3 Secretion System (T3SS) into plant cells to modulate host physiology and promote pathogenicity. The repertoire of T3Es present in a given strain largely influences its niche, host range and lifestyle. In the Xcc strain 8004, twenty-eight genes have been predicted to encode proteins secreted by the T3SS. The functions of most Type 3 Secreted Proteins (T3SPs) within plant cells remain elusive. In this project, different strategies were approached to characterize the biological functions of the T3SPs of Xcc strain 8004 in plant cells. In the first chapter, we showed that the loss of individual T3SPs did not cause a significant effect on Xcc virulence on Arabidopsis. Yet, the heterologous expression of individual T3SPs in Arabidopsis plants revealed many T3SPs with marked effects on plant growth and transcriptome. Several T3SPs also triggered plant immune responses and some exhibited ambivalent activities by simultaneously inhibiting flg22-triggered phosphorylation of MPK3/6. In the second chapter, we conducted a comparative analysis of the in planta functions of the T3E XopAG and RipO1 which are encoded by orthologous genes in Xcc strain 8004 and Ralstonia solanacearum strain GMI1000 respectively. In our experiments, XopAG showed a significant contribution to Xcc pathogenicity that was not related to the suppression of some basal immune responses. XopAG and RipO1 exhibited functional similarities. Indeed, both T3E affected the expression of genes responsive to auxin, jasmonic acid and ethylene suggesting that both effectors inhibit plant growth. Finally, we made some efforts to identify the plant target of XopAG. An in silico search followed by pathogenicity assays posits BRG3 (BOI-RELATED GENE 3) as a candidate target of XopAG. In a parallel approach, we performed a suppressor screen to identify suppressor mutations that alleviate the growth defect induced by XopAG in Arabidopsis plants, resulting in eight suppressor lines. These provide a valuable opportunity to identify the pathways targetted by XopAG in Arabidopsis. Altogether, this project contributes to the better comprehension of the biological activities exerted by the Xcc strain 8004 T3SPs in planta
Björnfot, Ann-Catrin. "Meticulous control of the T3SS of Yersinia is essential for full virulence." Doctoral thesis, Umeå universitet, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-42577.
Full textPatel, Samir. "Characterization of the caspase-3 cleavage motif of the Salmonella Typhimurium effector protein SifA and its role in pathogenesis." eScholarship@UMMS, 2018. https://escholarship.umassmed.edu/gsbs_diss/1002.
Full textFerreira, Rafael Marini [UNESP]. "Secretoma da bactéria fitopatogênica Xanthomonas citri subsp. citri." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/92688.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O cancro cítrico está entre as principais doenças que afetam a produção de laranjas no Brasil e é causado pela bactéria fitopatogênica gram-negativa Xanthomonas citri subsp. citri (Xac). O presente trabalho teve por objetivo analisar a expressão diferencial de proteínas secretadas pela bactéria selvagem e por um mutante (02H02) assintomático, que teve a proteína HrpB4, que participa de seu sistema de secreção tipo III (SSTT) inativada, em condição de cultivo em meio rico CN e em meio XAM1 indutor de hipersensibilidade e patogenicidade (genes hrp). As proteínas secretadas em meio de cultura foram extraídas pela ação do ácido tricloroacético (TCA) e identificadas através de espectrometria de massas. Tais análises identificaram 55 proteínas diferentes secretadas em ambos os meios de cultura, tanto para Xac quanto para 02H02, de modo que 13 destas proteínas são comuns entre a Xac e seu mutante cultivados em XAM1 e 14 são exclusivas para Xac cultivada em XAM1, as quais deixaram de ser secretadas no 02H02. Proteínas relacionadas aos genes reguladores do SSTT foram detectadas em condição infectante para ambas as bactérias, demonstrando a eficácia do meio de cultura XAM1 em induzir Hrp. Foi observado que diversas proteínas secretadas pelo sistema de secreção tipo II (SSTD) em condição infectante para Xac e seu mutante possuem um papel ativo na degradação das paredes celulares do hospedeiro e podem ser reguladas por proteínas controladoras do SSTT. Fatores de sinalização difusíveis produzidos por Xac aparentemente sofreram alteração em sua secreção no mutante devido à inativação do pilus do SSTT, demonstrando a relação dessa molécula com o SSTT. A não detecção de proteínas secretadas diretamente pelo SSTT denota que as mesmas podem estar sendo secretadas no interior de vesículas lipídicas de membrana externa, assim como ocorre em Xanthomonas campestris
Citrus canker is among the major diseases which affect citrus production in Brazil and is caused by the gram-negative phytopathogenic bacterium Xanthomonas citri subsp. citri (Xac). This work aimed to analyze the differential expression of secreted proteins by the wild bacterium and by an asymptomatic mutant (02H02), lacking the type III secretion system (TTSS) protein HrpB4, in rich cultivation medium NB and in the hrp inducing medium XAM1. The proteins secreted in all culture media have been extracted by trichloroacetic acid based protocols (TCA) and identified using mass spectrometry. The analysis identified 55 different proteins secreted in both culture medium for Xac and 02H02, of which 13 are common among Xac and its mutant cultivated in XAM1 and 14 proteins are exclusively secreted by Xac cultivated in XAM1. Proteins related to the TTSS regulatory genes have been detected in infecting condition in both bacteria, showing the effectiveness of XAM1 hrp inducing medium. It has been observed that several type II secretion system’s secreted proteins showed an active role in host cell wall degradation and may be regulated by type III secretion system’s proteins in Xac and 02H02 in infecting condition. Diffusible signal factors produced by wild Xac apparently suffered an altered secretion in the mutant due the inactivation of the type three secretion system’s pilus, showing the relationship of this molecule with this secretion system. The lack of detection of proteins secreted by the TTSS denote that these proteins may be secreted in the interior of outer membrane lipid vesicles, just like it was verified in Xanthomonas campestris
Ferreira, Rafael Marini. "Secretoma da bactéria fitopatogênica Xanthomonas citri subsp. citri /." Jaboticabal : [s.n.], 2009. http://hdl.handle.net/11449/92688.
Full textAbstract: Citrus canker is among the major diseases which affect citrus production in Brazil and is caused by the gram-negative phytopathogenic bacterium Xanthomonas citri subsp. citri (Xac). This work aimed to analyze the differential expression of secreted proteins by the wild bacterium and by an asymptomatic mutant (02H02), lacking the type III secretion system (TTSS) protein HrpB4, in rich cultivation medium NB and in the hrp inducing medium XAM1. The proteins secreted in all culture media have been extracted by trichloroacetic acid based protocols (TCA) and identified using mass spectrometry. The analysis identified 55 different proteins secreted in both culture medium for Xac and 02H02, of which 13 are common among Xac and its mutant cultivated in XAM1 and 14 proteins are exclusively secreted by Xac cultivated in XAM1. Proteins related to the TTSS regulatory genes have been detected in infecting condition in both bacteria, showing the effectiveness of XAM1 hrp inducing medium. It has been observed that several type II secretion system's secreted proteins showed an active role in host cell wall degradation and may be regulated by type III secretion system's proteins in Xac and 02H02 in infecting condition. Diffusible signal factors produced by wild Xac apparently suffered an altered secretion in the mutant due the inactivation of the type three secretion system's pilus, showing the relationship of this molecule with this secretion system. The lack of detection of proteins secreted by the TTSS denote that these proteins may be secreted in the interior of outer membrane lipid vesicles, just like it was verified in Xanthomonas campestris
Orientador: Jesus Aparecido Ferro
Coorientador: Julio Cezar Franco de Oliveira
Banca: Maria Teresa Marques Novo
Banca: Leandro Márcio Moreira
Mestre
Namdari, Fatémeh. "Caractérisation fonctionnelle de BamB, protéine impliquée dans la biogénèse de la membrane externe et la virulence de Salmonella." Thesis, Tours, 2013. http://www.theses.fr/2013TOUR4005/document.
Full textBamB is an outer-membrane lipoprotein belonging to the BAM complex (β-Barrel Assembly Machinery). In Salmonella, it is involved in the assembly of outer membrane proteins (OMP), in antibiotic susceptibility, in the transcriptional control of the three Type-Three-Secretion-Systems (T3SS) related genes and also in virulence. In E. coli, BamB interacts directly with the BamA protein. Moreover, BamB has been shown to have a serine-threonin kinase activity in this bacterium. In order to better characterize the roles of the BamB protein, our purposes were to study (1) the impact of the alteration of the interaction of BamB with the BAM complex or of its cytoplasmic sequestration and (2) its putative kinase activity in Salmonella. Our results show that some of the BamB roles are dissociable and that the BamA/BamB interaction is not required for T3SS expression, Salmonella virulence or OMP assembly in the outer membrane. Currently, neither a kinase activity nor a cytoplasmic activity has been clearly demonstrated for this protein
Thomas, William J. "Identification and characterization of type III effector proteins in plant-associated bacteria." Thesis, 2012. http://hdl.handle.net/1957/29206.
Full textGraduation date: 2012
Brena, Mariana. "Studies on the Interaction and Organization of Bacterial Proteins on Membranes." 2019. https://scholarworks.umass.edu/masters_theses_2/759.
Full textJorgensen, Ine. "The Chlamydia trachomatis Protease CPAF Regulates Secreted Bacterial Effectors and Host Proteins Essential to Virulence." Diss., 2011. http://hdl.handle.net/10161/3832.
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In recent years, many new chlamydial effector proteins have been described. CPAF (Chlamydial Protease-like Activity Factor) is a secreted serine protease that is emerging as a central virulence protein: it is proposed to play a central role in
Dissertation