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1

Bostanian, Noubar J., Martin Trudeau, and Jacques Lasnier. "Management of the two-spotted spider mite, Tetranychus urticae [Acari: Tetranychidae] in eggplant fields." Phytoprotection 84, no. 1 (January 9, 2004): 1–8. http://dx.doi.org/10.7202/007439ar.

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Summary A three-season field evaluation showed that eggplants (Solanum melongena) are very tolerant to damage caused by the two-spotted spider mite, Tetranychus urticae. Although large numbers of the predacious mites, Neoseiulus fallacis or Phytoseiulus persimilis were released, biological control of the two-spotted spider mite could not be sustained in the field. Based on a preliminary comparison of yield in both sprayed and unsprayed plots and on a three-year qualitative assessment, a tentative action threshold of 600 two-spotted spider mites per leaf would not decrease yield and would reduce the number of acaricide treatments per season. The new acaricide spirodiclofen was effective against the two-spotted spider mite in a preliminary trial.
2

Gillman, Jeffrey H., Michael A. Dirr, and S. Kristine Braman. "Gradients in Susceptibility and Resistance Mechanisms of Buddleia L. Taxa to the Two-spotted Spider Mite (Tetranychus urticae Koch)." Journal of the American Society for Horticultural Science 124, no. 2 (March 1999): 114–21. http://dx.doi.org/10.21273/jashs.124.2.114.

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Buddleia taxa were assessed for two-spotted spider mite (Tetranychus urticae Koch) resistance using a leaf disk bioassay, a novel shell vial bioassay and a field trial. Leaf pubescence and chemistry were examined for their role in two-spotted spider mite resistance. Results from bioassays and field sampling identified highly resistant taxa including B. fallowiana Balif. `Alba' and B. davidii × B. fallowiana Franch. `Cornwall Blue' as well as susceptible taxa including B. davidii Franch. `African Queen' and B. lindleyana Fort. ex Lindl. `Gloster'. The shell vial bioassay was an accurate predictor of field resistance to spider mite. Leaf pubescence was quantified by calculating the collective length of trichome branches per square millimeter of leaf surface area [effective branch length (EBL)]. EBL values ranged from 39 to 162 mm·mm-2 of leaf surface area among Buddleia taxa. Resistance was positively correlated with increased pubescence. Removal of pubescence by peeling resulted in increased oviposition of two-spotted spider mites. Exposing female two-spotted spider mites to a methylene chloride extract of B. davidii × B. fallowiana `Cornwall Blue' using a modified shell vial bioassay resulted in reduced oviposition and a methylene chloride extract of B. davidii `African Queen' resulted in no difference in oviposition when compared with a control. While pubescence is the best indicator of resistance to the two-spotted spider mite in Buddleia taxa, it is possible that defensive compounds are involved.
3

Langston, William C. "Two Spotted Spider Mite Control, 1990." Insecticide and Acaricide Tests 16, no. 1 (January 1, 1991): 186. http://dx.doi.org/10.1093/iat/16.1.186.

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Abstract This test was designed to compare ABG-6277A to Kelthane for control of two-spotted spider mite. Delta Pine 50 was planted 8 May in plots 8 rows × 40 ft long. The plots were arranged in a randomized complete block design with 4 replications. The plots were infested with TSSM by placing one infested bean plant every 3 steps along the center 2 rows of each plot on 29 Jun. Mite predators were reduced by weekly sprayings of Asana 0.025 lb (AI)/acre beginning on 28 Jun. Treatments were applied 11 and 18 Jul with a Hi Boy sprayer with tips every 20 inches delivering 10 gpa. The percent mite infested leaves in the 5th and 6th position from the terminal was determined by examining 25 leaves on the center 2 rows of each plot. Sampling dates were 18 and 24 Jul and 1 and 7 Aug. Yield was not measured because of leaf blight which partially defoliated plots.
4

Szafranek, Piotr. "Occurrence of Two-Spotted Spider Mite (Tetranychus urticae Koch) on Potentilla fruticosa Cultivars." Journal of Horticultural Research 24, no. 2 (December 1, 2016): 25–30. http://dx.doi.org/10.1515/johr-2016-0017.

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Abstract Potentilla fruticosa L. (Rosaceae Juss.) is one of the most popular deciduous shrubs cultivated in Poland. Among pests identified so far on P. fruticosa, a serious threat is caused by two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae). During three-year study, the occurrence of two-spotted spider mite T. urticae on four cultivars of P. fruticosa: ‘Goldfinger’, ‘McKay’s White’, ‘Uman’ and ‘Pink Beauty’ grown in field conditions was compared. While conducting experiment, weather conditions were monitored. The number of eggs and motile forms of two-spotted spider mite were recorded separately. The most attractive cultivar to spider mite was ‘Goldfinger’. The largest amount of motile forms of mites and their eggs were found on the leaves of this cultivar in all three years of study. The least number of pests occurred on the cv. ‘Pink Beauty’. However, differences in the abundance of this pest on all studied cultivars were variable during the growing seasons.
5

Vidrih, Matej, Anja Turnšek, Magda Rak Cizej, Tanja Bohinc, and Stanislav Trdan. "Results of the Single Release Efficacy of the Predatory Mite Neoseiulus californicus (McGregor) against the Two-Spotted Spider Mite (Tetranychus urticae Koch) on a Hop Plantation." Applied Sciences 11, no. 1 (December 24, 2020): 118. http://dx.doi.org/10.3390/app11010118.

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In 2015, we studied the efficiency of the predatory mite Neoseiulus californicus (McGregor) for suppression of the two-spotted spider mite Tetranychus urticae in a field experiment at a hop plantation. We randomly arranged four treatments in a three-block experiment. Fungicides were used in all treatments; insecticides were used in all treatments except the predatory mite treatment and acaricides were used in only two treatments. A single inundative release of the mite N. californicus was carried out on 4 July. On four different dates (10 June, 17 July, 29 July and 9 August), we counted the eggs and the mobile stages (larvae, nymphs and adults) of the two-spotted spider mite in all four treatments. In the treatment with the predatory mite, we established the fewest eggs and mobile stages of T. urticae 14 days after the release of the predator. The selected acaricides in our research acted in a primarily ovicidal manner, but we did not detect satisfactory effects on the mobile stages of the two-spotted spider mite. This result suggests the emergence of resistance of two-spotted spider mites to the acaricides hexythiazox and abamectin. Our research established comparable effects of the predatory mite N. californicus and acaricides, and further improvement of the efficiency would require release of the natural enemy into a hop plantation in mid-June, followed by a second release three weeks later. The costs of acaricide use in our experiment were from 12.7-fold (two sprayings of hexythiazox, and a single spraying with abamectin) to 17.8-fold (single treatments of hexythiazox and abamectin) lower than those of a single release of the biological control agent in question. The results of our study represent a starting point for future research, which could achieve satisfactory results in suppressing two-spotted spider mites on a hop plantation by repeated use of the predatory mite N. californicus.
6

Gillman, Jeff, Michael Dirr, and Kristine Braman. "Factors Affecting the Resistance of Buddleia Species to the Two-Spotted Spider Mite." HortScience 32, no. 4 (July 1997): 595A—595. http://dx.doi.org/10.21273/hortsci.32.4.595a.

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Buddleia species are attractive ornamental shrubs whose major cultural problem is infestations of the two-spotted spider mite. Two factors, drought stress and leaf pubescence, were examined for their effects on spider mite infestations. Drought stress was examined by placing 20 plants into a randomized block design and stressing ten of the plants. No differences in mite life parameters were found, although there was more uninfested area in nonstressed plants. This was probably due to the nonstressed plants' ability to outgrow the spider mite infestation. Leaf texture was examined by placing four female spider mites on 5 leaf disks of 37 cultivars and species of Buddleia and counting the number of eggs laid after 96 hours. There was a strong correlation between leaf pubescence and number of eggs laid. In a second experiment, using a shell vial with a leaf placed underneath the lid and five female spider mites in the vial itself, leaf pubescence was either removed with facial peel or left. In all cases but one, the removal of pubescence increased the number of eggs laid by the spider mites.
7

Salmane, Ineta. "Effect Of Agrotechnical Measures And Varieties On Seasonal Dynamics Of Tetranychus Urticae Koch (Acari, Trombidiformes, Tetranychidae) On High Tunnel-Cultivated Garden Strawberries." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 69, no. 1-2 (April 1, 2015): 45–49. http://dx.doi.org/10.1515/prolas-2015-0005.

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Abstract The aim of the present study was to determine the seasonal pattern of two-spotted spider mite Tetranychus urticae on strawberries cultivated in polythene-covered high tunnels in temperate climatic conditions. Various cultivars were used and the effect of modification of plant covering indices on abundance and incidence of these mites was also tested. The number of two-spotted spider mites was relatively low at the beginning of the vegetation season and started to increase when average air temperature rose above 20 to 25 °C. In the experiment two types of tunnels differing in additional plant cover were used. The maximum mite abundance did not significantly vary between varieties in tunnel 1 conditions, but it was significantly lower for variety 'Sonata' in tunnel 2 conditions. Mite numbers significantly declined after strawberry foliage mowing and removal of polythene cover. Mite development was prolonged in tunnel 1, where additional cover of plants was used and higher early season air temperature was recorded in comparison to tunnel 2. It was concluded that increase in early season temperature can increase two-spotted spider mite abundance and have a more negative effect on strawberry plants in respect of foliage damage by mites.
8

Konopacki, Paweł J., and Wojciech Warabieda. "Assessment of Dimensions in Order to Aid Classification of European Red Mite and Two-Spotted Spider Mite by Means of Digital Imaging of Infected Leaves." Agricultural Engineering 22, no. 4 (December 1, 2018): 15–24. http://dx.doi.org/10.1515/agriceng-2018-0032.

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AbstractThe methods currently used to assess orchard infestation are time-consuming and do not take into account non-adult forms of mites, due to their small size. Advance in digital imaging technology, however, has resulted in the discovery of a more viable method to enable a fast and reliable assessment of orchard infestation. Digital images of infected apple leaves were made and examined for the presence of European red mites and two-spotted spider mites. As well as adult mites, non-adult European red mite forms were also encountered. After extracting all objects considered as mites from the image, imaging software was then used to assess their dimension and shape parameters. Length, width, area, and equivalent diameter were different for all three observed mite groups: adult female European red mites, possible non-adult European red mites and adult two-spotted spider mites. Differences in circularity and elongation were found in adult two-spotted spider mites and various forms of European red mites, while the perimeter was similar in adult European red mites and two-spotted spider mites, and different in the non-adult forms of European red mites. However, as the ranges of 95% prediction intervals for these features overlap, a combination of at least two features as well as employing statistical procedures may greatly increase the probability of appropriate classification of the observed objects as different mites.
9

Doğan, Salih, Mustafa Çağlar, Bülent Çağlar, Çağrı Çırak, and Erhan Zeytun. "Structural characterization of the silk of two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae)." Systematic and Applied Acarology 22, no. 4 (April 18, 2017): 572. http://dx.doi.org/10.11158/saa.22.4.11.

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The present investigation was undertaken to obtain information about the structural and morphological properties of the silk of two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) by means of the atomic force microscope (AFM), scanning electron microscope (SEM), energy dispersive X-ray spectrophotometer (EDX) and attenuated total reflection Fourier transmission infrared spectroscopy (ATR-FTIR) techniques. AFM images show that the shapes of silken threads produced by both female and male mites are similar and have non-uniform cylindrical structure. In general, the appearance of silks produced by female mites is smoother than those of the male mites. In addition, it is determined that the silk threads produced by both female and male individuals have single and double stranded structure. There were differences in the thread dimensions of female and male which could depend on the body size of the mites. However, there is no statistically significant correlation between the body size of the mites and their thread dimensions. The silk material was found to consist predominantly of C, N and O elements and minor fractions of Al, P, Cl and K. The ATR-FTIR spectrum of the silk of two-spotted spider mite exhibited characteristic vibrational modes originating from amid groups, which were attributed to random coil and β-sheet molecular conformation in the protein structure of the silk of two-spotted spider mite. Understanding the features of the two-spotted spider mite silks may be of practical importance for the future studies related to biomaterial and material science.
10

Grazzini, Richard, Donald Walters, Jody Harmon, David J. Hesk, Diana Cox-Foster, June Medford, Richard Craig, and Ralph O. Mumma. "Inheritance of Biochemical and Morphological Characters Associated with Two-spotted Spider Mite Resistance in Pelargonium ×hortorum." Journal of the American Society for Horticultural Science 122, no. 3 (May 1997): 373–79. http://dx.doi.org/10.21273/jashs.122.3.373.

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Diploid zonal geraniums (Pelargonium ×hortorum) are able to resist attack by small arthropod pests such as the two-spotted spider mite (Tetranychus urticae Koch) when exudate produced by tall glandular trichomes contains a high percentage of ω5-unsaturated anacardic acids. Trichomes of susceptible plants exude primarily saturated anacardic acids. Inbred mite-resistant and -susceptible geraniums were reciprocally crossed and the F1, F2, and backcross generations were examined for anacardic acid composition and trichome density. Selected F2 plants were bioassayed for resistance to two-spotted spider mites. High concentrations of ω5-unsaturated anacardic acids in resistant plants are conditioned by a single dominant allele. We propose that inheritance of tall glandular trichome density can be controlled by a small number of loci (possibly as few as one) exhibiting codominance. F2, with low densities of tall glandular trichomes and producing ω5-unsaturated anacardic acids, displayed effective resistance to two-spotted spider mites as measured by mite mortality and fecundity. A genetic model for the biosynthesis of anacardic acids is proposed.
11

Pulga, Paulo Sérgio, Juliane Maciel Henshel, Juliano Tadeu Vilela de Resende, André Ricardo Zeist, Aline Fabiana Paladini Moreira, André Gabriel, Mayara Barbosa Silva, and Leandro Simões Azeredo Gonçalves. "Salicylic acid treatments induce resistance to Tuta absoluta and Tetranychus urticae on tomato plants." Horticultura Brasileira 38, no. 3 (September 2020): 288–94. http://dx.doi.org/10.1590/s0102-053620200308.

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ABSTRACT This study was performed to evaluate the effect of exogenous application of salicylic acid as a resistance inducer to two-spotted spider mite and tomato pinworm on tomato genotypes. Bioassays were carried out in three commercial genotypes (‘Redenção’, ‘Giuliana’, and ‘Alambra’) and one wild genotype (Solanum habrochaites var. hirsutum, accession PI-127826). Average distance travelled by the two-spotted spider mite and number of eggs deposited over the leaflets of the genotypes were investigated. Concerning pinworm bioassay, number of eggs, leafminers on leaflet surface and percentage of leaf area consumed by leafminers were evaluated using a scanner. Furthermore, severity of attack to plants was evaluated by score scale for damages, types of lesions and attacked leaflets. Salicylic acid application induced resistance in the commercial genotypes against both pests, reducing the two-spotted spider mite and pinworm oviposition. The spider mites travelled shorter distance on leaflets of genotypes treated with salicylic acid at intermediate concentrations, indicating a positive effect of substance as inducing resistance. Attack severity by pinworm was also reduced when intermediate doses of salicylic acid were applied. Also, higher resistance was observed on accession PI-127826 as compared to other genotypes. The obtained results indicate that the resistance of PI-127826 was due to constitutive defense, whereas on commercial genotypes defense was dependent on responses mediated by salicylic acid. Hence, salicylic acid treatment, as resistance inducer, seems to be efficient against two-spotted spider mite and pinworm, being a promising tool within an integrated pest management on tomato plants.
12

Sparks, Thomas C., John R. Skomp, Jon Babcock, and Chaoxian Geng. "Biogenic amines in the two-spotted spider mite." Archives of Insect Biochemistry and Physiology 25, no. 1 (1994): 1–8. http://dx.doi.org/10.1002/arch.940250102.

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Kersten, Anne-Katrin, Carmen Büttner, and Peter Lentzsch. "Determination of spider mite abundance in soil of field-grown cucumbers and in plants under predatory mite pressure in invasive infestations using HRM real-time PCR assay." PLOS ONE 17, no. 7 (July 14, 2022): e0270068. http://dx.doi.org/10.1371/journal.pone.0270068.

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The two spotted spider mite, Tetranychus urticae Koch L. (Acari: Tetranychidae), is a plant pest that can lead to severe economic losses in open field cucumber cultivation. Between 2017 and 2019 we studied the abundance of spider mites in the soil to estimate the potential infestation pressure of soil colonizing spider mites. The spider mites were heterogeneously distributed in small concentrations in the soil. Soil colonizing spider mites did not affect spider mite abundance on plants and reversed. We observed that spider mite migration occurred primarily from the edge of the field adjacent to the weed strip. In 2020 and 2021, we investigated the efficacy of the predatory mite Neoseiulus californicus (McGregor) for suppressing spider mite hotspots in the cropland. We compared untreated spider mite hotspots with N. californicus treated hotspots and showed that a single release of predatory mites could result in a high level of control when spider mite infestation density was initially high. With this study, soil can be ruled out as a habitat for spider mites, and attention to spider mite pest control can be directed to plant infestations. The highly sensitive HRM real-time PCR assay was used for the quantification of the spider mites.
14

Ribeiro, Edenilson B., Maria Aparecida Castellani, Daniel Júnior de Andrade, Renato de M. Prado, Larissa Adelita SC Souza, Daniel S. Freire, and Patrice Jacob Savi. "Biological aspects of the two-spotted spider mite on strawberry plants under silicon application." Horticultura Brasileira 39, no. 1 (March 2021): 5–10. http://dx.doi.org/10.1590/s0102-0536-20210101.

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ABSTRACT Silicon is an inducer of plant resistance to arthropod pests, being a promising strategy for integrated management. The aim of this study was to evaluate the effect of silicon on biological, reproductive and population aspects of parental and F1 generations of the two-spotted spider mite on strawberry plants. Potassium silicate, nanosilica and water were applied to the plants. Two-spotted spider mite females were confined to strawberry leaf disks for oviposition and, after hatching, larvae were observed until the emergence of adults. Once adults had been obtained, couples were formed in order to evaluate pre-oviposition, oviposition, longevity and fertility, with an estimated net reproduction rate, intrinsic rate of increase, finite rate of increase and the necessary time for doubling the generation. Silicon prolonged the duration of some immature stages of the mites in parental and F1 generations, did not affect the duration of the whole biological cycle, though. The periods of pre-oviposition, oviposition and longevity of the parental generation and the longevity and oviposition of F1 generation of the two-spotted spider mite were negatively affected by potassium silicate and nanosilica. The population parameters of the parental generation of the mites indicated that nanosilica is able to lead to a long-run decrease of this pest population.
15

Abd-Alla, Hisham I., and Saad E. S. Hamouda. "Study of potential activity of clove oil 10 % emulsifiable concentrate formulation on Two-spotted spider Mite Tetranychus urticae Koch (Acari: Tetranychidae)." Journal of Applied and Natural Science 13, no. 4 (December 16, 2021): 1414–19. http://dx.doi.org/10.31018/jans.v13i4.3124.

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Clove oil is reported to have a great range of biological activities against many species of pests; termites, cockroaches, aphids, weevils and moths, and many different usages such as perfume and food flavoring agent. This study aimed to determine the acaricidal activity of clove oil emulsifiable concentrate new formulation against two-spotted spider mites Tetranychus urticae. Clove oil was formulated as 10 % emulsifiable concentrate (EC). The new formula passed successfully all physical and chemical tests reported for emulsifiable concentrates. It was then tested biologically on the individuals of two-spotted spider mite T. urticae Koch (Acari: Tetranychidae) on two host plants beans and squash under laboratory conditions. In both cases, there were a direct relationship between the increase in the concentration of the formulation and the percentage of inhibition on the individuals of the two-spotted spider mite, after 72 hours of treatment, at concentrations of 20, 40, and 80 mg/ml the new formulation showed 10.30, 44.8, and 75 percent inhibition, respectively in case of beans, while squash at the same concentrations and for the same period of treatment showed 33.3, 40.7, and 51.9 percent inhibition, but the effect in case of beans was greater than that in case of squash as its EC50 value was lower than that in case of squash. In the case of the former, it was 39.81 mg/ml, whereas in the case of the latter, it was 79.43 mg/ml. Therefore, the new clove oil formulation can be used to combat the two-spotted spider mite.
16

Beers, E. H., and R. W. Browne. "Apple, European Red Mite and Two Spotted Spider Mite Control, 1992." Insecticide and Acaricide Tests 18, no. 1 (January 1, 1993): 1–2. http://dx.doi.org/10.1093/iat/18.1.1a.

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Abstract This test was conducted in an orchard with trees of mixed ages near Malaga, Washington. The experimental design was randomized complete block with nine treatments and four single tree replications. All trees were treated with Asana 0.66 EC at 2 oz/100 gal, 200 gal/acre on 18 Jun to eliminate predatory mites. Blocks were based on pre-treatment counts on 30 Jun. Test materials were applied on 2 Jul. Per acre rates were based on 400 gal/acre dilute application. Treatments were applied with a handgun operated at 300 psi, sprayed to the point of drip. Population density samples were 25 leaves per tree. Leaf samples were brushed onto glass plates with a leaf-brushing machine and mites were counted under a dissecting microscope.
17

Modarres Najafabadi, Seyed Saeid, Abdoolnabi Bagheri, and Majeed Askari-Seya Hooei. "Cucumber cultivar responses to two tetranychid mites, two-spotted spider mite and strawberry spider mite in greenhouses ." Systematic and Applied Acarology 24, no. 8 (August 8, 2019): 1383–93. http://dx.doi.org/10.11158/saa.24.8.4.

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Cultivating resistant host plants has been considered as an important strategy for sustainable pest management of mites. The current study was conducted to evaluate the response of 43 cucumber (Cucumis sativus L.) cultivars against two important tetranychid species, two-spotted spider mite (Tetranychus urticae Koch.) and strawberry spider mite (Tetranychus turkestani Ugarov & Nikolski). The experiment was implemented in a Randomized Complete Block design (RCB) with 43 treatments replicated 4 times in a greenhouse during 2015-2016. Two main evaluation criteria, cultivar injury rate (from 1 to 5) and number of eggs/female/day (EFD) on each cultivar, were used to compare responses. The results revealed that the highest number of eggs/female/day (EFD) for both mites was observed on Taha cultivar (9.0±0.40 and 9.5±0.28 for T. urticae and T. turkestani, respectively). The highest injury rate was also observed on this cultivar (4.8±0.26 and 5.0±0.0 for T. urticae and T. turkestani, respectively). In contrast, the lowest EFD number for T. urticae (2.8±0.47) and T. turkestani (2.5±0.28) was seen on Samer star and Davos cultivars, respectively. However, no host preference for egg-laying was observed on Iver and Samer star cultivars for T. turkestani. These two cultivars had the lowest injury level too (1.2±0.25 for Samer star and 1.5±0.28 for Negeen to T. urticae and T. turkestani, respectively). The obtained results demonstrated that Taha was the most susceptible cultivar colonized easily by both two tetranychid species. In contrast, Samer star and Negeen were the most resistant cultivars, strongly recommended for cultivation in greenhouses.
18

Nielsen, D. G., and M. J. Dunlap. "Cotoneaster, Control of Two-Spotted Spider Mite, Wooster, Ohio, 1989." Insecticide and Acaricide Tests 16, no. 1 (January 1, 1991): 250. http://dx.doi.org/10.1093/iat/16.1.250.

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Abstract Cotoneaster growing in 1-gal (3.9 1) containers in a greenhouse at the main OARDC campus were used to evaluate efficacy of selected pesticides against two-spotted spider mites. A CO2 compression sprayer at 20 psi with a single Teejet SS8004 flat-fan nozzle was used to apply treatments to runoff. Infested plants were selected at random and placed in groups of 3 replicates/treatment. The first application was applied to all plants 25 Sep; the second application to selected plants 2 Oct. Plants were hand-watered every Mon, Wed, and Fri throughout the test period. Treatments were evaluated 1, 2, 4, and 8 wk after the second application by removing five, 10-13 cm long branch tips from each of the 3 replicates/treatment. Samples were placed in plastic bags and transported, in coolers, to the laboratory. All samples were processed by passing them through a mite brusher 6 times to dislodge mites to a rotating glass plate coated with Tween-20. Dislodged mites were counted with the aid of a binocular dissecting microscope.
19

Nam, Myeong Hyeon, Hyun Sook Kim, InHa Lee, Jeong Hak Seo, and Byung Joo Lee. "Suppression of Powdery Mildew and Two-Spotted Spider Mite by UV-B Radiation and Mulching Type of Strawberry Cultivation in the Greenhouse." Research in Plant Disease 28, no. 2 (June 30, 2022): 61–68. http://dx.doi.org/10.5423/rpd.2022.28.2.61.

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Powdery mildew and two-spotted spider mite are detrimental to strawberry plants and are controlled with traditional pesticides. To accommodate consumer demand, eco-friendly methods of pest control are required. Strawberries were cultivated (in soil and in a hydroponic system) for two years, and ultraviolet B (UV-B) irradiation was used as an alternative pest control during the harvesting season. Three varieties were grown (Seolhyang, Kingsberry, and Durihyang), and four UV-B lamp/mulch (black, green, and light reflection sheet [LRS]) combinations were used during harvesting: UV-B+black or green mulch, UV-B+black or green+LRS, no UV-B+black or green, and no UV-B+black or green+LRS. In all varieties, powdery mildew was 65% more controlled when UV-B irradiation was used. The adult two-spotted spider mite density was lowest in the UV-B lamp+black or green+LRS treatments. Therefore, UV-B irradiation during the strawberry harvesting season could effectively control powdery mildew and two-spotted spider mite with little side effect on the plants.
20

OHZORA, Yousuke, and Shuichi YANO. "Fertilization of Two-Spotted Spider Mite Mothers Changes Sons." Journal of the Acarological Society of Japan 17, no. 2 (2008): 87–92. http://dx.doi.org/10.2300/acari.17.87.

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Raworth, David A. "Control of Two-spotted Spider Mite by Phytoseiulus persimilis." Journal of Asia-Pacific Entomology 4, no. 2 (November 2001): 157–63. http://dx.doi.org/10.1016/s1226-8615(08)60117-x.

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Zanin, Daniel S., Juliano TV Resende, André R. Zeist, João RF Oliveira, Juliane M. Henschel, and Renato B. Lima Filho. "Selection of processing tomato genotypes resistant to two spotted spider mite." Horticultura Brasileira 36, no. 2 (June 2018): 271–75. http://dx.doi.org/10.1590/s0102-053620180221.

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ABSTRACT One of the major problems in cultivation of tomato is the occurrence of pests and diseases. The objective of the research was to select genotypes from the second generation of the first backcross (F2BC1) between the tomato cultivar for processing S. lycopersicum (cv. Redenção) and the wild access S. habrochaites var. hirsutum (PI-127826), with high levels of zingiberene (ZGB), resistant to the twospotted spider mite Tetranychus urticae (Acari: Tetranychidae). From the F2BC1 population. The ZGB content was quantified and five plants with high zingiberene selected (RVTZ 2011-079-117, RVTZ 2011-079-185, RVTZ 2011-079-335, RVTZ 2011-079-345 and RVTZ 2011-079-503) and four with low zingiberene content (RVTZ 2011-079-417, RVTZ 2011-331-460, RVTZ 2011-079-538 and RVTZ 2011-079-548) were selected. Genotypes selected for high and low ZGB content and the parentals S. habrochaites var. hirsutum access PI-127826 and ‘Redenção’ were evaluated for repellency to the twospotted spider mite. There was a significant and inverse correlation between ZGB content and average distances travelled by mites on tomato leaflets. The genotypes of the F2BC1 generation with high zingiberene levels RVTZ 2011-079-117, RVTZ 2011-079-185, RVTZ 2011-079-335, RVTZ 2011-079-345 and RVTZ 2011-079-503 are promising for progress in achieving resistant lines to arthropod pests. Among them RVTZ 2011-079-117 stands out for resistance to twospotted spider mite.
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Gillman, Jeffrey H., Mark W. Rieger, Michael A. Dirr, and S. Kristine Braman. "Drought Stress Increases Densities but Not Populations of Two-spotted Spider Mite on Buddleia davidii `Pink Delight'." HortScience 34, no. 2 (April 1999): 280–82. http://dx.doi.org/10.21273/hortsci.34.2.280.

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Two experiments were conducted to determine the effect of drought stress on the susceptibility of Buddleia davidii Franch. `Pink Delight' to the two-spotted spider mite (Tetranychus urticae Koch). In the first experiment, drought stress was imposed by withholding water until predawn xylem pressure potential fell below -1 MPa. Shoot growth was 75% less in drought-stressed than in nonstressed plants. Mite population densities were not affected, but noninfested leaf area was 14% higher, and degree of mite damage was lower, in nonstressed plants. Evidently, the greater amount of new growth in nonstressed plants leads to lower spider mite densities by diluting populations. In a second experiment, nonstressed B. davidii `Pink Delight' plants were watered every 1 to 2 days and drought-stressed plants were watered every 3 days. Spider mite populations were monitored by sampling newly expanded and mature foliage. Mite populations on mature foliage were not affected by stress, but stressed plants grew less and had larger spider mite populations on their newly expanded foliage than did nonstressed plants.
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Jęcz, Tomasz, Małgorzata Korbin, Damian Gorzka, Abdel-Rahman A. Mohamed, and Remigiusz Olszak. "Molecular Diversity of Two-Spotted Spider Mite (Tetranychus urticae) in Apple Orchards Across Poland and its Potential Link with the Resistance to Meti-Acaricides." Journal of Horticultural Research 26, no. 2 (December 1, 2018): 103–11. http://dx.doi.org/10.2478/johr-2018-0021.

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AbstractTwo-spotted spider mite (Tetranychus urticae, Koch) possess tremendous ability to develop resistance to pest control chemicals. Hence, although METI-acaricides are quite new group of pesticides, the resistance to this chemicals may shortly become a serious obstacle in two-spotted spider mite management. In presented study, molecular and phenotypic diversity of three laboratory strains and seven T. urticae populations collected from pest-protected apple orchards were analysed in reference to METI-acaricides resistance. The inter-population molecular diversity analysis with a set of RAPD markers revealed 6 populations to be in close relation to susceptible reference strain (GSS). Only one population turned out to be linked relatively close with METI-acaricides resistant strain (Akita). Simultaneously, the results of biological test showed similar relation among the analysed populations, according to the resistance incidence ratio. Obtained results suggest that METI-acaricides resistance trait in two-spotted spider mite is likely to be determined at genomic level and may be considered as an initial stage for developing molecular biology tools for early detection of METI-acaricides resistance in T. urticae populations.
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Garcia-Garcia, D., E. Guerrero-Rodriguez, and J. Landeros-Flores. "Acaricides Against Two Spotted Spider Mite in Zamora, Michoacan, Mexico, 1990." Insecticide and Acaricide Tests 18, no. 1 (January 1, 1993): 69–70. http://dx.doi.org/10.1093/iat/18.1.69a.

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Abstract Materials were applied on 28 Mar on 4m furrow plots (20 plants); 7 acaricides in a randomized complete block design with an untreated furrow buffer strip around each plot. Acaricides were applied at a rate of 600 liter/ha, using a motorized sprayer 121 liter capacity. ‘Chandler’ strawberry variety was used when plants had 250 days post transplant under irrigation at El Valenciano ranch and there were no unusual conditions during the test, temperatures were generally warm without rain. There were 8 samples post treatment every 3 days. The number of mites were counted from 5 randomly chosen plants and 5 leaves/plant for each replication (100 leaflets/treatment). Natural populations of spider mites were high and reasonably well distributed. Data were analyzed by Duncan’s multiple range test, and by comparing pretreatment means with post-treatmenlt means using the Henderson and Tilton formula. The spider mite species present during the test was identified by mounting them on a glass slide for microscopic examination.
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Chen, Jianjun, Richard J. Henny, Lance S. Osborne, Russell D. Caldwell, and Cynthia A. Robinson. "004 Thrips (Hercinothrips femoralis) and Two-spotted Spider Mite (Tetranychus urticae) Resistance of Anthurium Cultivars under Interior Conditions." HortScience 35, no. 3 (June 2000): 388C—388. http://dx.doi.org/10.21273/hortsci.35.3.388c.

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Potted anthurium is becoming an important indoor flowering foliage plant because of its unique attractive appearance and continuous growth and flowering under interior conditions. However, an interior environment, with controlled optimal temperatures and relative humidity and living plants, is an ideal niche for pest development. Pests such as thrips and two-spotted spider mite on Anthurium have been great challenges to the interiorscape industry because many pesticides have been rigorously restricted for interior use. Thus, exploiting the genetic potential of cultivar resistance may be the best approach for the control of these pests. In this study, eight of the most popular Anthurium cultivars were evaluated for their resistance to a natural infestation of thrips (Hercinothrips femoralis) and two-spotted spider mite (Tetranychus urticae) under three light levels: 4, 8, and 16 μmol·m-2·s-1, temperatures of 23.8 to 26.7 °C and a relative humidity of 60%. Results indicated that significant resistant differences exist among cultivars. The cultivars most resistant to thrips were not the most resistant to mite and vice versa. Cultivars that exhibited moderate resistance to thrips were also moderately resistant to mite. Low light intensity appeared to be a factor influencing thrips infestation since control plants that grew under a light intensity of 200 μmol·m-2·s-1 had no observed thrips damage. On the other hand, two-spotted spider mite infestation was not influenced by light intensity.
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Favaro, Renata, Juliano Tadeu V. Resende, André Gabriel, André R. Zeist, Ely Cristina N. Cordeiro, and Jorge Luís Favaro Júnior. "Salicylic acid: resistance inducer to two-spotted spider mite in strawberry crop." Horticultura Brasileira 37, no. 1 (March 2019): 60–64. http://dx.doi.org/10.1590/s0102-053620190109.

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ABSTRACT The strawberry is susceptible to several pests which cause a reduction in productivity. The use of mechanisms which repel or prevent these pests to establish, represent a sustainable environmental technology to reduce the frequency of agrochemical use. In this context, the aim of this study was to evaluate the effect of salicylic acid (SA) on resistance induction against two-spotted spider mite in strawberry cultivars (Aromas and Sweet Charlie). Five concentrations of SA (0, 25, 50, 75 and 100 mg L-1) were tested in order to verify the antixenosis effects. In SA concentration of 50 mg L-1, a reduced number of mites in the two strawberry cultivars was noticed. For the number of eggs deposited on leaflet, we verified an effect of SA concentration with quadratic adjustment in the equation. Sweet Charlie cultivar was more effective than Aromas in relation to the reduction of mite oviposition on leaflets treated with SA. SA concentrations of 25, 50 and 75 mg L-1 were the most efficient for the evaluated traits allowed to infer its potential as antixenotic resistance inducer against two-spotted spider mite in strawberry crop.
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Baskaran, V., E. Sumathi, M. Vignesh, and S. V. Krishnamoorthy. "Eco-friendly management of two spotted spider mite, Tetranychus urticae (Koch) in Brinjal." Ecology, Environment and Conservation 29 (2023): S1—S6. http://dx.doi.org/10.53550/eec.2023.v29i01s.001.

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The effect of the application of acaropathogenic fungi viz., Beauveria bassiana, Nomuraea anisopliae, Lecanicillium lecanii, Hirsutella thompsonii and biopesticides viz., azadirachtin 1500 ppm, azadirachtin 3000 ppm, azadirachtin 10000 ppm and Fortified Neem oil were tested with synthetic acaricides like Propargite and Fenazaquin against two-spotted spider mite (Tetranychus urticae). The result of evaluation of acaropathogenic fungi revealed that low mite population of 5.68 nos/2cm2 was observed in Fenazaquin 10 EC @ 1.5ml/l applied plot with 69.78% reduction in mite population followed by Propargite and Nomuraea anisopliae treated plots and the result of evaluation of botanicals revealed that the application of Azadiractin 10000 ppm @ 2 ml/l resulted in low mite population of 6.75 nos /2 cm2 with 64.11% reduction over control. Therefore, the present investigation outlines the efficacy of plant extracts and acaropathogenic fungi as bio pesticide to display toxicity against two-spotted spider mite on brinjal increasing in yield and avoid environmental pollution and consumer health hazards.
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Godzina, Monika, Małgorzata Kiełkiewicz, and Katarzyna Szymczykiewicz. "Varying abundance and dispersal of the two-spotted spider mite (Tetranychus urticae Koch, 1836, Acari: Prostigmata: Tetranychidae) on Mi-tomato plants differing in allelic combination." Biological Letters 48, no. 2 (January 1, 2011): 213–23. http://dx.doi.org/10.2478/v10120-011-0022-8.

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Varying abundance and dispersal of the two-spotted spider mite (Tetranychus urticaeKoch, 1836, Acari: Prostigmata: Tetranychidae) onMi-tomato plants differing in allelic combinationIt is well known that the presence of theMi-1.2gene determines the resistance of tomato plants (Solanum lycopersicumL.) to 3 root-knot nematode species and some insect herbivores of the order Hemiptera. In this study, the density and dispersal of two-spotted spider mites (Tetranychus urticae) on tomato plants of 4 cultivars were evaluated under greenhouse conditions, to find out whether tomatoes differing in allelic combination of theMi-1.2gene exhibit similar or different resistance to the mite pest. The results show, for the first time, that the same initial number of mites develops into populations that vary in abundance and distribution depending on tomato allelic composition of theMi-1.2gene. The results indicate that the mite-pest develops more slowly on tomato plants of cultivar ‘Motelle’, carrying 2 dominant alleles for theMi-1.2gene, than on heterozygous ‘Altess F1’ tomatoes and both cultivars carrying only recessive alleles of this gene: ‘Moneymaker’ and ‘Plaisence F1’. This suggests that theMi-1.2gene may be involved in tomato resistance against spider mites when this dominant gene is expressed homozygously, but this needs to be verified by further research.
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Peixoto, Joicy V. M., Ana L. A. Ribeiro, Gabriel M. Maciel, Camila S. de Oliveira, Rafael R. Finzi, and Emmerson R. de Moraes. "Productivity, acylsugar concentrations and resistance to the two-spotted spider mite in genotypes of salad tomatoes." Revista Brasileira de Engenharia Agrícola e Ambiental 24, no. 9 (September 2020): 596–602. http://dx.doi.org/10.1590/1807-1929/agriambi.v24n9p596-602.

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ABSTRACT The objective of this study was to characterize genotypes of the salad-type tomato with a determinate growth habit and select genotypes resistant to the two-spotted spider mite for higher productivity and fruit quality. The experiment was conducted at the Horticultural Experimental Station (18°42’43.19” S and 47°29’55.8” W, 873 m altitude) of the Universidade Federal de Uberlândia (UFU), Monte Carmelo campus, from January to June 2018. The experimental design was in randomized blocks containing 16 treatments [12 populations of the F3RC2 generation and four controls: two commercial cultivars (Santa Clara and Rio Grande), the recurrent parent (UFU-057) and the wild species Solanum pennellii (LA-716)] with three repetitions. The accessions UFU-057F3RC210#3.6.1 and UFU-057F3RC213#2.1.2 have high potential for genetic improvement regarding resistance to the two-spotted spider mite. The genotypes UFU-13#4.4.1, UFU-7#4.7.1, UFU-7#4.3.2, UFU-13#2.5.2 and UFU-13#2.1.3 present higher acylsugar concentrations than S. pennellii, in addition to higher productivity and number of fruits per plant. It is suggested to explore combinations between those genotypes previously described with UFU-8#2.4.1 to obtain hybrids with high acylsugar concentrations, two-spotted spider mite resistance, high productivity and increased fruit mass.
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Crenna, Ana Cecilia, Claudio Marcelo Oddino, Santiago Ferrari, Jorge Antonio Giuggia, Diego Giovanini, and Francisco Damián Giordano. "Development and validation of diagrammatic scales to evaluate damage by the two-spotted spider mite (Tetranychus urticae) in peanut." Revista de la Facultad de Ciencias Agrarias UNCuyo 53, no. 1 (July 7, 2021): 254–61. http://dx.doi.org/10.48162/rev.39.024.

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Argentina is the second largest peanut (Arachis hypogaea L.) exporter in the world. The main peanut pest in our country is the two-spotted spider mite (Tetranychus urticae Koch 1836). To date, there is no validated method to quantify this pest. The aim of this work was to develop and validate a logarithmic diagrammatic scale to assess damage by the two-spotted spider mite in peanut. In 2015-16, 200 leaflets were collected from a plot infested with the pest. Damaged leaf area (DLA) was calculated using SisCob software. Six-, seven-, eight- and nine-class scales were proposed. Mean values for each class and scale were obtained with 2-LOG. Leaflets were analyzed by 13 raters who used the four proposed scales. Precision and accuracy were determined by simple linear regression between the DLA and estimated damaged leaf area. Reproducibility was determined by linear regression between estimates of raters combined in pairs. The seven-class scale was the best validated one for all the parameters. Most raters showed constant deviations and overestimated DLA, whereas only one rater presented systematic deviations. This seven-class scale is the first developed and validated one to evaluate two-spotted spider mite damage to peanut in Argentina. Highlights According to the validation results, the seven-class diagrammatic scale was the best one in terms of accuracy, precision and reproducibility with values above 0.80, 0.75 and 0.75 respectively. The scales are useful tools for pest assessment, fundamental parameters for integrated pest management. The seven-class scale is the first one developed and validated for evaluating damage by the two-spotted spider mite in peanut crop in Argentina. With this scale a loss function will be calculate which will allow the estimation of the EIL of this pest.
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Nielsen, D. G., and M. J. Dunlap. "Cotoneaster, Control of Two-Spotted Spider Mite, Lake County, Ohio, 1989." Insecticide and Acaricide Tests 16, no. 1 (January 1, 1991): 250–51. http://dx.doi.org/10.1093/iat/16.1.250a.

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Abstract Cotoneaster growing in 1-gal (3.91) containers at a nursery in Lake County were used to evaluate efficacy of selected pesticides against two-spotted spider mites. A C02 compression sprayer at 20 psi with a single Teejet SS8004 flat-fan nozzle was used to apply treatments to runoff. Infested plants were selected at random and placed in groups of 6 replicates/ treatment. The first application was applied to all plants 24 Jul when conditions were sunny, 30°C, with 8-16 kph winds; second applications were made to selected plants 3 Aug with conditions sunny, 30°C, and 16-24 kph winds. Treatments were evaluated 1, 2, and 3 wk after second application by removing five, 10-13 cm long branch tips from each of the 6 replicates/treatment. Samples were placed in plastic bags and transported, in coolers, to the laboratory. All samples were processed by passing them through a mite brusher 6 times to dislodge mites to a rotating glass plate coated with Tween-20. Dislodged mites were counted with the aid of a binocular dissecting microscope.
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Warabieda, Wojciech, and Remigiusz W. Olszak. "The influence of the two-spotted spider mite (Tetranychus urticae Koch.) and irrigation on yield of 5-years-old apple trees." Acta Agrobotanica 55, no. 2 (2013): 113–19. http://dx.doi.org/10.5586/aa.2002.049.

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The influence of the two-spotted spider mite (<i>Tetranychus urticae</i>) on yield of apple trees was investigated. The experiment was conducted on rrigated and non-irrigated 5-years-old apple trees of 'Close' and 'Jonagold' cultivars grafted on M.9. The population size of the pest exceeded four and two times the valid (in Poland) economic injury level on trees of 'Close' and 'Jonagold' cultivars respectively. No significant influence of mites on yield and its quality (mean fruit weight and fruit colour) was observed on both apple cultivars. Irrigation had positive effect on yield and quality of 'Jonagold'. On the contrary, trees of ' Close' cultivar were positively influenced by irrigation only regarding fruits colour. Results of the study support opinion that the economic injury level for apple orchards, with respect to two-spotted spider mite, should be higher than it is up to now.
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Nielsen, D. G., and M. J. Dunlap. "Cotoneaster, Control of Two-Spotted Spider Mite, Lake CO., Ohio, 1990." Insecticide and Acaricide Tests 17, no. 1 (January 1, 1992): 342. http://dx.doi.org/10.1093/iat/17.1.342.

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Abstract Cotoneaster growing in 3 gal (11.7 liter) containers at a nursery in Lake Co., OH, were used to evaluate efficacy of selected pesticides against two-spotted spider mites. A CO2 compression sprayer at 30 psi with a single Teejet SS8004 flat-fan nozzle was used to apply treatments to run-off Infested plants were selected at random and placed in groups of 4 replicates per treatment. The first application was applied to all plants 3 Jul under sunny skies, 27°C, and 16-24 km/h winds; the second application to selected plants was on 13 Jul under cloudy skies, 19°C, and 16-24 km/h winds. Plants received over-head irrigation every other day or as needed. Treatments were evaluated 6 and 24 days after the second application by removing four, 10-13 cm long branch tips from each of the 4 replicates per treatment. Samples were placed in plastic bags and transported, in coolers, to the laboratory. All samples were processed by passing them through a mite brusher 6 times to dislodge mites to a rotating glass plate coated with Tween-20. Dislodged mites were counted with the aid of a binocular dissecting microscope.
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Hammad, E. Abou-Fakhr, M. Akkary, N. Saliba, M. Farran, and S. Talhouk. "Bioactivity of Indigenous Medicinal Plants against the Two-Spotted Spider Mite, Tetranychus urticae." Journal of Agricultural Science 9, no. 7 (June 7, 2017): 123. http://dx.doi.org/10.5539/jas.v9n7p123.

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Forty two methanol extracts and 12 aqueous extracts of 29 indigenous medicinal plant species were tested for their acaricidal bioactivity against the two spotted spider mite, Tetranychus urticae adults at the laboratory. Fourteen methanol plant extracts caused significant mortality in mites. This is the first report for the potential effect on survival of mites for 27 tested endemic plant species. Methanol whole plant extracts (WPEs) of Lotus carmeli, Alchemilla diademata, Eryngium deserlorum and aqueous fruit extracts (FrEs) of Melia azedarach caused toxic effects against the adult mites in the range of 41-46% mortality. The methanol WPE of L. carmeli and the aqueous FrE of M. azedarach (1:5) caused the highest mite mortality of 43.55% and 45.55%, respectively and each was used as reference sample for potential acaricidal activity in the methanol and aqueous treatment groups. The latter extract was not significantly different in its effect from aqueous extracts of M. azedarach leaf extract (LE) and Achillea damascena WPE. Methanol extracts of Salvia rubifolia flowers and Calendula palestina FrE were found to be more active against the adult mite than their extracts of other plant parts as leaves and flowers, respectively. The former two extracts, flower extracts of Anthemis scariosa, Echinops gaillardoti, Nepeta curviflora, and Ranunculus cuneatus, leaves and stems extract of An. scariosa and WPEs of Melissa inodora, Ranunuculus myosuroides, Origanum libanoticum and Ac. damascena were found to be comparable in their acaricidal activity to that of the whole plant extract of L. carmeli. Thus, these bioactive extracts of some local plant species can cause toxicity to adult T. urticae mites and consequently could be an alternative control for mite pests.
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Sadras, V. O., and L. J. Wilson. "Nitrogen accumulation and partitioning in shoots of cotton plants infested with two-spotted spider mites." Australian Journal of Agricultural Research 48, no. 4 (1997): 525. http://dx.doi.org/10.1071/a96146.

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In cotton (Gossypium hirsutum L.), leaves are the main site of nitrogen reduction and constitute a large reservoir of organic nitrogen. Foliar herbivores, therefore, are likely to have detrimental effects on the nitrogen economy of the plant. A field experiment was conducted to investigate the effects of two-spotted spider mites (Tetranychus urticae Koch) on the accumulation and partitioning of nitrogen in cotton shoots. Control plants and plants infested with mites 3 times in the growing season were compared. Once established, mite colonies grew exponentially. After peaking at 35–64 mites/leaf, mite numbers declined sharply. Mites markedly affected both shoot nitrogen accumulation and partitioning. The amount of nitrogen in shoots of mite-infested plants peaked earlier than in controls, and reached maximum values that were 50–69% of the controls. Early infestation (at the onset of reproductive growth) had a greater effect than infestations initiated during active reproductive growth. The concentration of nitrogen in leaves declined faster in mite-infested plants than in controls, mostly due to accelerated leaf senescence. This rapid decline in leaf nitrogen concentration could be one of the factors involved in the collapse of mite colonies in heavily infested plants. Nitrogen concentration of stems and reproductive organs was generally greater in mite-infested plants than in controls. Allometric analysis showed that this was related to both the small size of mite-infested plants, and true increases in nitrogen content probably associated with translocation from senescing leaves.
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Reddall, A., V. O. Sadras, L. J. Wilson, and P. C. Gregg. "Physiological Responses of Cotton to Two-Spotted Spider Mite Damage." Crop Science 44, no. 3 (2004): 835. http://dx.doi.org/10.2135/cropsci2004.0835.

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Suzuki, Takeshi, Masakatsu Watanabe, and Makio Takeda. "UV tolerance in the two-spotted spider mite, Tetranychus urticae." Journal of Insect Physiology 55, no. 7 (July 2009): 649–54. http://dx.doi.org/10.1016/j.jinsphys.2009.04.005.

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Clotuche, Gwendoline, Anne-Catherine Mailleux, Shuichi Yano, Claire Detrain, Jean-Louis Deneubourg, and Thierry Hance. "Settlement decisions by the two-spotted spider mite Tetranychus urticae." Comptes Rendus Biologies 336, no. 2 (February 2013): 93–101. http://dx.doi.org/10.1016/j.crvi.2013.02.006.

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Astudillo Fernandez, Aina, Thierry Hance, Gwendoline Clotuche, Anne-Catherine Mailleux, and Jean Louis Deneubourg. "Testing for collective choices in the two-spotted spider mite." Experimental and Applied Acarology 58, no. 1 (April 28, 2012): 11–22. http://dx.doi.org/10.1007/s10493-012-9558-5.

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Reddall, A., V. O. Sadras, L. J. Wilson, and P. C. Gregg. "Physiological Responses of Cotton to Two‐Spotted Spider Mite Damage." Crop Science 44, no. 3 (May 2004): 835–46. http://dx.doi.org/10.2135/cropsci2004.8350.

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Monteiro, L. B., T. M. A. Kuhn, A. F. Mogor, and E. D. B. da Silva. "Biology of the Two-Spotted Spider Mite on Strawberry Plants." Neotropical Entomology 43, no. 2 (January 11, 2014): 183–88. http://dx.doi.org/10.1007/s13744-013-0184-7.

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Suekane, Renato, Paulo Eduardo Degrande, Elmo Pontes de Melo, Thiago Ferreira Bertoncello, Izidro dos Santos de Lima Junior, and Cássio Kodama. "Damage level of the two-spotted spider mite Tetranychus urticae Koch (acari: tetranychidae) in soybeans." Revista Ceres 59, no. 1 (February 2012): 77–81. http://dx.doi.org/10.1590/s0034-737x2012000100011.

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Among phytophagous spider mites, the two-spotted spider mite Tetranychus urticae Koch, 1836 is one of the most important agricultural pests, not only because of the damage it causes, but also because it has a wide host range, infesting many commercial crops such as leafy greens, cotton, beans, and soybeans, among others. This study was carried out in a greenhouse of the Faculdade de Ciências Agrárias (FCA) of the Universidade Federal da Grande Dourados (UFGD), located in the city of Dourados, state of Mato Grosso do Sul. The experiment was arranged in a randomized block design with 5 treatments and 4 replicates. The treatments consisted of 5 levels in percentage of chlorotic symptoms (indicating mite damage): 0%, 25%, 50%, 75%, and 100%. All of the characteristics evaluated, except for number of pods per plant, the number of seeds per plant, the total weight (productivity), and the weight of 1000 seeds, were significantly influenced by the different levels of chlorotic symptoms. The economic damage level for the two-spotted spider mite Tetranychus urticae, according to the equation y = 66.63-0.51 x, based on the price of US$ 11.00 per bag of soybeans and a control cost of US$ 16.00, would be 15.80% chlorotic symptoms. At a price of US$ 29.00 per bag with the same control cost, the economic damage level would be 13% of chlorotic symptoms.
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Walsh, Douglas B., and Gary G. Grove. "Repellency and Repulsiveness of Selected Agrichemicals to the Two-Spotted Spider Mite (Tetranychus urticae) on Grape Foliage." Plant Health Progress 6, no. 1 (January 2005): 1. http://dx.doi.org/10.1094/php-2005-1228-01-rs.

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Leaf disk and whole leaf bioassays were conducted to evaluate the repellency and repulsiveness of selected fungicides, insecticides, spray adjuvants, and petroleum-derived spray oils (PDSO) to the two-spotted spider mite, Tetranychus Urticae Koch (Acari: Tetranychidae). Compounds were evaluated for their ability to stimulate the movement of T. urticae from treated to nontreated grape tissue (repellency) and to inhibit their movement from untreated to treated tissue (repulsiveness). All insecticides other than Bacillus thuringiensis and some fungicides were highly repellent. Several insecticides and fungicides, and all spray adjuvants, were highly repulsive. We conclude that many agrichemicals are irritating to two-spotted spider mites and may impact their spatial dynamics within plant canopies. Accepted for publication 7 November 2005. Published 28 December 2005.
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Martel, Catherine, Vladimir Zhurov, Marie Navarro, Manuel Martinez, Marc Cazaux, Philippe Auger, Alain Migeon, et al. "Tomato Whole Genome Transcriptional Response to Tetranychus urticae Identifies Divergence of Spider Mite-Induced Responses Between Tomato and Arabidopsis." Molecular Plant-Microbe Interactions® 28, no. 3 (March 2015): 343–61. http://dx.doi.org/10.1094/mpmi-09-14-0291-fi.

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The two-spotted spider mite Tetranychus urticae is one of the most significant mite pests in agriculture, feeding on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. Here, we describe timecourse tomato transcriptional responses to spider mite feeding and compare them with Arabidopsis in order to determine conserved and divergent defense responses to this pest. To refine the involvement of jasmonic acid (JA) in mite-induced responses and to improve tomato Gene Ontology annotations, we analyzed transcriptional changes in the tomato JA-signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. Overlay of differentially expressed genes (DEG) identified in def-1 onto those from the timecourse experiment established that JA controls expression of the majority of genes differentially regulated by herbivory. Comparison of defense responses between tomato and Arabidopsis highlighted 96 orthologous genes (of 2,133 DEG) that were recruited for defense against spider mites in both species. These genes, involved in biosynthesis of JA, phenylpropanoids, flavonoids, and terpenoids, represent the conserved core of induced defenses. The remaining tomato DEG support the establishment of tomato-specific defenses, indicating profound divergence of spider mite–induced responses between tomato and Arabidopsis.
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He, Jun, Harro J. Bouwmeester, Marcel Dicke, and Iris F. Kappers. "Genome-Wide Analysis Reveals Transcription Factors Regulated by Spider-Mite Feeding in Cucumber (Cucumis sativus)." Plants 9, no. 8 (August 11, 2020): 1014. http://dx.doi.org/10.3390/plants9081014.

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To gain insight into the regulatory networks that underlie the induced defense in cucumber against spider mites, genes encoding transcription factors (TFs) were identified in the cucumber (Cucumissativus) genome and their regulation by two-spotted spider mite (Tetranychusurticae) herbivory was analyzed using RNA-seq. Of the total 1212 annotated TF genes in the cucumber genome, 119 were differentially regulated upon spider-mite herbivory during a period of 3 days. These TF genes belong to different categories but the MYB, bHLH, AP2/ERF and WRKY families had the highest relative numbers of differentially expressed genes. Correlation analysis of the expression of TF genes with defense-associated genes during herbivory and pathogen infestation, and in different organs resulted in the putative identification of regulators of herbivore-induced terpenoid and green-leaf-volatile biosynthesis. Analysis of the cis-acting regulatory elements (CAREs) present in the promoter regions of the genes responsive to spider-mite feeding revealed potential TF regulators. This study describes the TF genes in cucumber that are potentially involved in the regulation of induced defense against herbivory by spider mites.
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El-Sayed, Salwa M., Nevin Ahmed, Samy Selim, Areej A. Al-Khalaf, Nihal El Nahhas, Shams H. Abdel-Hafez, Samy Sayed, Heba M. Emam, and Mervat A. R. Ibrahim. "Acaricidal and Antioxidant Activities of Anise Oil (Pimpinella anisum) and the Oil’s Effect on Protease and Acetylcholinesterase in the Two-Spotted Spider Mite (Tetranychus urticae Koch)." Agriculture 12, no. 2 (February 3, 2022): 224. http://dx.doi.org/10.3390/agriculture12020224.

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The two-spotted spider mite, Tetranychus urticae, also known as the red spider, is one of the most harmful pests in agriculture and causes large losses of many crops. These mites have rapidly developed a resistance to many chemical pesticides in recent years. In this study, the essential oil of seeds of the anise plant (Pimpinella anisum) was extracted by hydrodistillation, and the chemical composition of the oil was analyzed. The antioxidant activity of the volatile oil was determined by the DPPH radical scavenging assay. The acaricidal activity of the anise oil, a natural acaricide, was evaluated for its ability to protect green bean plants from mite injury. The two-spotted spiders were spread on green bean seedlings for 1 week; then, different plants were sprayed with different concentrations of anise oil (10, 20, 30, or 40 µL/L). Our results revealed that anethole was the major component of anise oil, at 53.23%. The acaricidal effect of the various concentrations on T. urticae was recorded after 24, 48, and 72 h of treatment. Our findings suggest that anise oil showed significant acaricidal activity against T. urticae in a dose- and time-dependent manner. Anise oil at a concentration of 40 µL/L killed 96.0% of the red spiders after 72 h. Also, all concentrations of anise oil inhibited acetylcholinesterase, and the spiders’ protease activity declined when the plants were treated with 30 or 40 µL/L of anise oil. The concentrations of 10 and 20 µL/L did not significantly affect the protease activity of T. urticae mites. We can conclude that anise oil exhibited acaricidal activity against T. urticae and that this was highly correlated with the inhibition of acetylcholinesterase and protease activities in the mites.
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Colijn, Annette C., and Richard K. Lindquist. "Effects of Moisture Stress on Two Spotted Spider Mite Populations, Tetranychus urticae Koch (Acari: Tetranychidae) in Schefflera (Brassaia actinophylla Endl.)." Journal of Environmental Horticulture 4, no. 4 (December 1, 1986): 130–33. http://dx.doi.org/10.24266/0738-2898-4.4.130.

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Studies were conducted to determine the effects of moisture stress and schleffera variety (Brasspia actinophylla Endl. and B. actinophylla ‘Amate’) on populations of twospotted spider mides (Tetranychus urticae Koch) grown under interior conditions. After approximately 2 weeks, spider mite populations were significantly higher on moisture stressed B. actinophylla as compared with unstressed plants. Populations on rewatered plants were intermediate. Moisture stress did not affect mite populations on the cultivar ‘Amate’ in a consistent manner. The cultivar ‘Amate’ was more mite resistant than B. actinophylla. Mites feeding on ‘Amate’ laid fewer eggs, had lower survival and slower developmental rates than those feeding on B. actinophylla. Leaves of ‘Amate’ were 67% thicker and had lower percent total nitrogen than B. actinophylla leaves.
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Esteca, Fernanda De Cássia Neves, Camila Do Nascimento Dainese, Luis Rodolfo Rodrigues, André Luiz Lourenção, Francisco Antonio Passos, and Gilberto José de Moraes. "Resistance of New Strawberry Genotypes to the Two-Spotted Spider Mite (Acari: Tetranychidae)." Journal of Agricultural Science 9, no. 8 (July 18, 2017): 119. http://dx.doi.org/10.5539/jas.v9n8p119.

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The two-spotted spider mite, Tetranychus urticae Koch, is one of the main strawberry pests in Brazil and many other countries. The objective of this study was to compare the resistance of eight strawberry genotypes (‘Albion’, ‘IAC Guarani’, ‘IAC Princesa Isabel’, ‘Oso Grande’, IAC T-0104, IAC 12, IAC 4 and IAC 1.13) to the two-spotted spider mite, by assessing injury level, biological parameters and host preference of the mite. To facilitate the interpretation of the results, leaf trichomes of each genotype were quantified. Thirty days after the artificial infestation of the genotypes with the mite, IAC T-0104, IAC 12 and ‘IAC Princesa Isabel’ showed the lowest injury levels. ‘IAC Princesa Isabel’, IAC 4 and IAC T-0104 were distinguished from other genotypes by the highest mean generation time (T) of the mite, while ‘IAC Guarani’ was distinguished by the lowest T value. Significantly lower values of intrinsic rate of increase (rm), finite rate of increase (λ) and net reproductive rate (Ro) were determined on ‘IAC Princesa Isabel’ and IAC 1.13. The mite showed preference for ‘IAC Guarani’, as inferred by the larger number of females when offered the chance to select the detached host leaflets. Trichome density was highest on IAC 1.13 and lowest on IAC T-0104, IAC 12, ‘Albion’ and ‘Oso Grande’. The results suggested that ‘IAC Princesa Isabel’, IAC T-0104 and IAC 12 are resistant, IAC 4 and IAC 1.13 are moderately resistant, and ‘IAC Guarani’, ‘Oso Grande’ and ‘Albion’ are susceptible to the mite.
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Maciel, Anilde Da Graça Sousa, Roseane Cristina Predes Trindade, Edmilson Santos Silva, and Márcia Daniela Dos Santos. "Potential predation and fecundity of Amblyseius aerialis fed two-spotted spider mite." Comunicata Scientiae 9, no. 4 (January 4, 2019): 748–53. http://dx.doi.org/10.14295/cs.v9i4.2529.

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The mites of the Phytoseiidae family stand out for the biological control of pest mites. The species Amblyseius aerialis (Muma, 1955) is a generalist mite, which can contribute to the natural regulation of pest mites. The objective of this study was to evaluate the potential for predation and fecundity of A. aerialis on the different phases of Tetranychus urticae (Koch, 1836) (Acari: Tetranychidae). A female of A. aerialis was confined on dish (3.0 cm in diameter) of jack bean leaves, Canavalia ensiformis (L.) DC. The discs were left in petri dish with water. Each T. urticae phase was offered individually to A. aerialis at densities: 2, 5, 10, 15, 20 and 30 mites / arena. The number of prey consumed and eggs laid by A. aerialis were evaluated every 24 hours for seven days, with removal of laid eggs by phytoseiid and dead prey and replacement of prey in the initial amount. The data were submitted to analysis of variance and the means were compared by Tukey test, applying the factorial (4x6). It was observed that with the increase of the density of T. urticae offered there was an increase in the predation of A. aerialis. When offered the nymph and adult the average number of eggs per day was higher, differing from egg and larva. Thus, A. aerialis can be a biological control agent of the T. urticae, reducing the population of the pest and maintaining its fecundity in all phases and densities offered.

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