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1
Závacký, Martin. "Tunel na trase rychlé železnice." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2016. http://www.nusl.cz/ntk/nusl-240102.
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The theme of this thesis is to create a conceptual study of the tunnel on high speed railway. Selection of suitable cross section and tunnelling method with considering of geological conditions of the site. Design of primary support and technological process of construction.
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Scheidt, João Eduardo Cardoso. "O problema do tunel de congelamento." [s.n.], 1996. http://repositorio.unicamp.br/jspui/handle/REPOSIP/306872.
Full textAbstract:
Orientador: Clovis Perin FilhoDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Matematica, Estatistica e Computação Científica
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Resumo: Túneis de congelamento são equipamentos utilizados pela industria alimentícia para o condicionamento térmico de produto tais como iogurte, sorvete, leite, carne e derivados. Assim, geralmente estão no fim do processo de produção de produtos altamente perecíveis. Quando um túnel representa m gargalo do processo, ele causa transtornos à produção que podem até interrompê-la. Este trabalho visa a abordagem do problema de operação eficiente e túneis de congelamento quanto ao aspecto de carregamento. Primeiramente abordamos o problema estático e suas relaxações e restrições. No problema estático determinístico, o instante de chegada das bandejas de produtos e o tempo de exposição necessário para seu condicionamento são conhecidos de antemão. Deve-se, então, programar por completo o carregamento do refrigerador definindo, para cada bandeja, em que nível do túnel deve ser colocada ou se deve ser rejeitada. Resultados teóricos são obtidos, particularmente para o caso (relaxado) do refrigerador de gavetas. Várias possibilidades de abordagem do problema estático são discutidas. Em seguida abordamos o problema dinâmico, onde não se dispõe do conhecimento prévio dos parâmetros de cada bandeja. Estes só ficam definidos a partir da chegada de cada bandeja, quando então a decisão quanto ao carregamento ou rejeição da bandeja deve ser tomada. Para o problema dinâmico são apresentadas várias políticas, que são testadas num caso real e em cenários hipotéticos nele baseados. Para avaliação de desempenho das políticas usamos limites' inferiores calculados com base no desenvolvimento teórico do problema estático. Os resultados são muito satisfatórios para uma classe de políticas a que chamamos de encadeamento.
Abstract: Freezing tunnels are food industry facilities for conditioning of meat, ice-cream, milk, yogurt, etc. They are very unwished botlenecks because they are positioned at the end of the production process of highly perishable items (low in-process inventory). The freezing tunnel loading problem is that of maximizing its production in terms of the amount of frozen trays of products. The purpose of this study is to model this problem and develop methods for solving its static and dynamic versions. In the static problem, the arrival time and the conditioning time of each tray are known in advance for the entire loading period. One should then completely schedule the loading of the tunnel. Each tray must be assigned to a position in the tunnel or be rejected. . We discuss several approaches and show some theoretical results for relaxed problems. In the dynamic problem, the arrival and the demand of trays are not known in advance. In this case, the decisions must take place at the arrival times. Several policies are developed. Some real case based scenarios are used for testing the policies. The study shows the potential gains with the application of some policies, mainly those ofthe class we named 'chain policies'.
Mestrado
Mestre em Matemática Aplicada
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Drahoš, Vojtěch. "Server pro automatickou konfiguraci IPv6 tunelu." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2011. http://www.nusl.cz/ntk/nusl-237063.
Full textAbstract:
One of the major problems of modern Internet is the upcoming depletion of addressing space in IPv4 protocol. This master thesis focuses on the study of network protocol IPv6, the possibilities of expanding this protocol in the Internet using the appropriate tunelling method and ways of tunnel management, especially the TIC protocol. Major goal of this work is using knowledge of protocols in a design and implementation of the server part of tunnel broker system, which uses mentioned tunneling method and is compatible with the open source client AICCU. Second part of this work deals with testing and pilot deployment of this system. Main benefit consists in creation an open source tunel broker system. Real deployment of this system is particularly advantage for Czech users as a low latency tunnel IPv6 Internet.
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Nekl, Jiří. "Rekonstrukce železničního tunelu." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2013. http://www.nusl.cz/ntk/nusl-226363.
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The diploma thesis engages in the reconstruction of railway tunnel Domasovsky that is situated at the track section Olomouc – Krnov. It illustrates the current state of the construction and the geotechnical conditons of the area of interest, it also presents the potencial solutions. The design is verified by stuructural analysis, the design documentation and technical report are also enclosed.
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Asszonyi, Ondřej. "Zpracování naměřených signálů z kavitačních experimentů." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-416450.
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This thesis focuses on problem with detection of cavitation in hydraulic systems and devices. Thesis works with data from cavitation tunnel experiment, where cavitation appeared on blade. It founds out if time records and their frequency spectrum is dependent on operating conditions. Data are examined by various statistic methods. All of that is then used in method called neural network.
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MUNGUIA, GALLEGOS ADRIANA PARTICIA. "PROCEDIMIENTO CONSTRUCTIVO DEL BOULEVERD REFORMA TUNEL ECHANOVE.TOLUCA EN EL AREA DE TUNEL PARA LA CALLE PROLONGACION LOMAS DE VISTA HERMOSO SUR EN CUAJIMALPA, DISTRITO FEDERAL." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2013. http://hdl.handle.net/20.500.11799/62591.
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El trabajo de memoria, lo justifica la experiencia personal adquirida en la construcción. Los
formatos, programas, planos y problemáticas reales de un caso, es información que servirá como
soporte para todos aquellos estudiantes, pasantes de ingenieros e ingenieros que deseen integrarse
a la obra pública sin fallar o conociendo de antemano que la construcción es un negocio que
necesita toda la dedicación posible para que sea tan remunerable como se planteó en el concurso.
Es la astucia, la técnica y la sociabilidad entre otras, características (necesarias en todos los niveles
y puestos de la construcción) muy apreciadas para la correcta ejecución de la obra.
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Caceres, Huambo Baltazar Nicolas. "Simulação e otimização de um secador industrial de tunel para frutas." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256434.
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Orientador: Florencia Cecilia MenegalliTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: o objetivo do presente estudo foi desenvolver um modelo matemático para um secador industrial de túnel para fi'utas, simulá-Ia e otimizar sua operação considerando a qualidade do produto como restrição principal na formulação do problema de otimização. Os secadores operam em forma semi-contínua e têm carrinhos e bandejas. O período de cada ciclo é determinado fazendo cumprir as especificações da umidade inicial e final do produto, de forma que cada carrinho com um número determinado de bandejas com fruta úmida seja alimentado em ciclos ao túnel enquanto outro que atingiu a umidade final é retirado do outro extremo do túnel. As configurações consideradas no estudo foram concorrente e contracorrente. O modelo que foi desenvolvido a partir dos balanços simultâneos de calor e massa para a fase sólida e gasosa, tanto para as bandejas (carrinhos) quanto para o túnel, é simples e apropriado para efeitos de otimização onde o tempo computacional é muito importante. O modelo foi aplicado para simular a secagem de uva Itália usando dados experimentais da cinética de secagem, ajustando-se os parâmetros de acordo aos requerimentos das equações do modelo. Os códigos de simulação e otimização foram desenvolvidosem FORTRAN 90 de Microsoft Fortran PowerStation. A não linearidade das equações do modelo e da restrição de qualidade da fruta, representada pela degradação de ácido ascórbico, conduziram ao desenvolvimento de um algoritmo para a solução do problema de otimização cuja função objetivo foi minimizar o consumo de energia por unidade de sólido seco, tempo do ciclo e temperatura máxima atingida pela fruta. Os resultados mostram as condições de operação ótimas para um número fixo de carrinhos no túnel e indicam que a configuração contracorrente é mais eficiente quando se avalia os requerimentos de calor por kg de sólido seco para uma degradação de ácido ascórbico aproximadamente constante. Igualmente, que se reduz o consumo de energia em ambas configurações quando comparado aos valores simulados sem otimizar.
Abstract: The non linearity of the equations of the model and the quality of the fruit, represented by the ascorbic acid degradation, leaded to develop an algorithm for the solution of the optimization problem which objective function was to minimize the energy consumption, cycle time and maximum temperature achieved by the fruit. Results show the optimal operation conditions for a fixed number of trucks in the tunnel and indicate that countercurrent configuration is more efficient when heat requirements per kg of dry solids is evaluated for approximately constant ascorbic acid degradation. Furthermore, energy consumption for both configurations is reduced when compared to non optimized simulation results.
Doutorado
Doutor em Engenharia de Alimentos
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Ballarta, Martínez Lisely Odalix. "Diseño de un tunel de enfriamiento rápido tipo californiano para mangos." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2016. https://hdl.handle.net/20.500.12672/5458.
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Publicación a texto completo no autorizada por el autorRealiza los cálculos necesarios para seleccionar los equipos frigoríficos de 01 túnel de enfriamiento rápido, considerando que la capacidad del túnel de pre-frio es de 10 ton., esto implica la selección de evaporador, compresor y demás. Para poder realizar la correcta selección de los equipos de refrigeración se requiere determinar correctamente los siguientes parámetros: carga térmica, temperatura de evaporación, dimensiones del túnel de enfriamiento, etc.
Trabajo de suficiencia profesional
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Anchamparuthy, Vahida Muhammed Ismail. "Vitrification of Bovine Oocytes." Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/25986.
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Cryopreservation of oocytes is a challenge. Studies were conducted to vitrify mouse zygotes and cumulus-intact bovine oocytes from follicles of different diameters, small (â ¤ 4 mm) and medium (4 to 10 mm), using nylon mesh. The specific goals were to assess changes in apoptotic gene expression (Fas-FasL, Bax, Bcl-2, and survivin) in conjunction with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and caspase assays. Mouse zygotes were exposed to increasing concentrations of ethylene glycol (EG), Ficoll-70 and sucrose in phosphate buffered saline (PBS) for vitrification on nylon mesh and plunged into liquid nitrogen. Warming resulted in 81.7% morphological survival. The rate of blastocyst formation was 59.9% for vitrified zygotes but, this was significantly lower than that of non vitrified embryos (66.2%). There was no difference in the hatching rates between groups. Both Fas and FasL mRNA were detected at the 4-cell and morula stages, suggesting Fas signaling was operational in early embryos. The level of expression of Bax mRNA tended to increase, while expression of survivin mRNA was not different for 2- and 4-cell embryos. Fragmented embryos showed an increase in Bax mRNA levels, while survivin mRNA level was reduced.
In the second experiment, vitrification of bovine oocytes was carried out. Pre-cooled cryovials resulted in 98.9% morphological survival. The oocytes from small and medium size follicles had a significant impact on cleavage (53.7 ± 1.6% vs. 43.8 ± 1.6%, respectively) and blastocyst rates were 16.9 ± 1.0% and 11 ± 1.2%, respectively. Follicle size for oocytes had no impact on the expression of apoptotic genes. The Fas-FasL and Bax-Bcl-2 mRNA showed increased expression after vitrification, but tended to decrease after 9 h of maturation. Yet, results from TUNEL and caspase assays did not support the evidence of the downstream apoptotic signaling pathway in embryos. The semen utilized for in vitro fertilization in both vitrified and control oocytes responded differently in the 4 tested bulls than their field fertility record. The altered transcriptional activities of apoptotic genes, Fas-FasL and Bax-Bcl-2, and survivin were indicative of possible apoptotic activity in vitrified embryos and oocytes subjected to in vitro fertilization.Ph. D.
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Kobielusz, Petr. "Městský okruh Blanka, tunel Královská obora - ražená část, stavebně technologická příprava stavby." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2013. http://www.nusl.cz/ntk/nusl-226100.
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My thesis is focused on process preparation and building construction technology of primary lining road tunnel, implemented in city part of Troja in the capital city Prague. Specifically it is a part of tunnel complex Blanka. The name of this part is Kralovská obora. If the all tunnel parts are built this tunnel complex Blanka can be complemented to already opened tunnels Zlíchovský, Mrázovka and Strahovský which are the part of city traffic circle. This technology preparation includes schedules and financial plans of the building, technological prescription for the primary tunnel lining, safety plan, control and trial/test plans, environmental plan, project of construction site, design of the main working machines, items budget of the north tunnel tube, coordination situation plan with wider relationships transport routes and technical report of building technological project.
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Stjepan, Galamboš. "Uticaj oblika i vrste aerodinamičke opreme privrednih motornih vozila na otpor vazduha." Phd thesis, Univerzitet u Novom Sadu, Fakultet tehničkih nauka u Novom Sadu, 2020. https://www.cris.uns.ac.rs/record.jsf?recordId=114836&source=NDLTD&language=en.
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Usavršavanje aerodinamike privrednim motornih vozila putem optimizacije aerodinamičkih dodataka u svrhu postizanja boljih aerodinamičkih performansi u vidu umanjenja sile otpora vazduha. Prostiranje vazdušne struje oko modela je unapređeno optimizacionim dodacima što se sve ogleda u smanjenoj potrošnji goriva privrednog motornog vozila. Osim virtuelnih simulacija računarske dinamike fluida, u radu je prikazana validacija rezultata putem eksperimentalnog merenja u vazdušnom tunelu.The improvement of commercial motor vehicle's aerodynamics through optimization process of aerodynamic equpments in order to achieve better aerodynamic performance in the form of drag force reduction. The expansion of the air flow around the model is enhanced by optimization accessories, which is all reflected in the reduced of fuel consumption of the commercial motor vehicle. In addition to virtual simulations of computational fluid dynamics, the paper presents the validation of results by experimental measurement in the wind tunnel.
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del, Pozo Blanco Olga. "Efectos sobre el dolor y la función de la terapia del espejo en el síndrome del túnel del carpo bilateral." Doctoral thesis, Universitat Rovira i Virgili, 2017. http://hdl.handle.net/10803/454720.
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La finalitat d'aquesta tesi és evaluar els efectes sobre el dolor i la funció que té la teràpia de l'espill al síndrom del túnel carpià bilateral, i a més, observar la relació entre el catastrofisme associat al dolor en la lesió, i els efectes de la tècnica.
Un total de 18 pacients derivats del Servei de Traumatologia de l'Hospital de Manises de València (Espanya), participaren a l'assaig clínic, els quals van ser inclossos aleatòriament entre el grup espill i el placebo. Els pacients van emplenar qüestionaris per a avaluar el dolor, la funció i el catastrofisme associat al dolor. Durant les vuit setmanes que va durar la intervenció, entre el tractament efectuat amb l'investigador i el domiciliari, els subjectes del grup d'intervenció realitzaren exercicis amb un espill per a tindre feedback visual, mentres els del placebo els efectuaven amb l'espill girat. Una vegada realitzat l'anàlisi de les dades recopilades, es va observar que, els pacients inclosos al grup espill mostraven una reducció del dolor major que els que havien estat al placebo. Pel contrari, no es va evidenciar que la teràpia de l'espill tinguera repercussió sobre la funció. De la mateixa manera, el catastrofisme associat al dolor es va relacionar amb la intensitat percebuda del dolor, però no va ser determinant sobre els efectes de la tècnica estudiada.
En conclusió, els resultats obtinguts ajuden a ampliar les estratègies de tractament habitualment emprades en rehabilitació, així como a aprofundir en el catastrofisme com a factor associat al dolor al síndrom del túnel carpià.La finalidad de esta tesis es evaluar los efectos sobre el dolor y la función que tiene la terapia del espejo en el síndrome del túnel del carpo bilateral, y además observar la relación entre el catastrofismo asociado al dolor en la lesión, y en los efectos de la técnica. Un total de 18 pacientes derivados del Servicio de Traumatología del Hospital de Manises de Valencia (España), participaron en el ensayo clínico, los cuales fueron incluidos aleatoriamente entre el grupo espejo y el placebo. Los pacientes rellenaron cuestionarios para evaluar el dolor, la función, y el catastrofismo asociado al dolor. Durante las ocho semanas que duró la intervención, entre tratamiento efectuado con el investigador y el domiciliario, los sujetos del grupo de intervención realizaron ejercicios con un espejo para tener feedback visual, mientras los del placebo los efectuaban con el espejo girado. Una vez efectuado el análisis de los datos recopilados, se observó que, aquellos pacientes incluidos en el grupo espejo mostraron una reducción del dolor mayor que aquellos que se encontraban en el grupo placebo. Por el contrario, no se evidenció que la terapia del espejo tuviera repercusión sobre la función. Del mismo modo, el catastrofismo asociado al dolor se relacionó con la intensidad percibida del dolor, pero no fue determinante sobre los efectos de la técnica estudiada. En conclusión, los resultados obtenidos ayudan a ampliar las estrategias de tratamiento habitualmente empleadas en rehabilitación, así como a profundizar sobre el catastrofismo al ser un factor asociado al dolor en el síndrome del túnel del carpo.
The aim of this thesis is to evaluate the effects on pain and function of mirror therapy in bilateral Carpal Tunnel Syndrome, and also to observe the relationship between catastrophism associated with pain in the lesion and the effects of the technique. A total of 18 patients from the Trauma Service of the Manises Hospital of Valencia (Spain) participated in the clinical trial, which were randomly included between the mirror and placebo groups. Patients completed questionnaires to assess pain, function, and catastrophism associated with pain. During the eight weeks of the intervention, between investigator and home treatment, the subjects in the intervention group performed exercises with a mirror to have visual feedback, while those of the placebo performed with the mirror turned. After analyzing the collected datas, it was observed that those patients included in the mirror group showed a greater pain reduction than those who were in the placebo group. On the contrary, it was not evidenced that the mirror therapy had repercussion on the function. Similarly, catastrophism associated with pain was related to the perceived intensity of pain, but was not determinative of the effects of the technique studied. In conclusion, the results obtained help to broaden the treatment strategies commonly used in rehabilitation, as well as to deepen the catastrophism as a factor associated with pain in the carpal tunnel syndrome.
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Bernuy, Torres Andrea Estela. "Sintomatología del síndrome del túnel carpal en odontólogos." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2007. https://hdl.handle.net/20.500.12672/2203.
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La función motora y sensitiva de la mano es la herramienta básica principal en el ejercicio de la odontología, toda actividad clínica odontológica necesita el adecuado funcionamiento de la mano para realizar una labor eficiente.
El Síndrome del Túnel Carpal es un desorden producido por una compresión nerviosa, que tiene entre sus principales factores de riesgo muchas de las actividades o ejercicios manuales a los que un odontólogo se somete en su ejercicio común.
Este síndrome ha sido estudiado anteriormente en países extranjeros donde se ha encontrado en muchos casos mayor prevalencia en la comunidad odontológica sea de profesionales, estudiantes, higienistas dentales y/o asistentes dentales que en la población general. Es, por lo tanto, de relevante importancia el reconocimiento y establecimiento de su sintomatología en nuestra realidad, por lo que la presente investigación se sitúa entre los primeros estudios basales que sobre esta enfermedad ocupacional se realizan en nuestro país.Tesis
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Alarcón, Llange Néstor Robert. "Correlación clínica y confirmación quirúrgica en el síndrome del pronador." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2004. https://hdl.handle.net/20.500.12672/1445.
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Se realizó un estudio descriptivo, retrospectivo, transversal; la Unidad de muestreo fueron los pacientes con diagnostico clínico de Síndrome del Pronador sometidos a Liberación Quirúrgica en el Departamento de Ortopedia y Traumatología del Hospital Nacional Edgardo Rebagliati Martins entre Junio del 2003 y Mayo del 2004. Se realizó además la liberación quirúrgica del Ligamento Transverso del Carpo en todos los pacientes con cuadro clínico de compresión del Túnel del Carpo asociado al Síndrome del Pronador. El análisis estadístico se realizó con el programa SPSS Vs. 11. Fueron seleccionados 26 casos, se encontró que 73 % (19) eran mayores de 45 años y el 69.2% (18) de sexo femenino, el 76.9% (20) se dedicaban a labores que se relacionaban a actividades manuales, el 88.5 % (23) eran diestros y en el 65.4 % (17) el atrapamiento nervioso se encontró en la extremidad derecha, en un 84.6 % (22 ) se realizó una liberación del Síndrome del Túnel del Carpo homolateral. Respecto de las maniobras clínicas, el 46.2% (12) presentaron dolor en el punto pronador, seguido de un 23.1 % (06) supinación contra resistencia, y un 23.1% (06) pronación contra resistencia; los hallazgos quirúrgicos indicaron que en el 42.3 % (11) fue la aponeurosis bicipital, en un 30.8 % (08) la banda del pronador redondo y en un 19.2 % (05) causas tumorales las responsables de la compresión. Las complicaciones postoperatorias, fueron hematoma 30.8 % ( 08 ), e infección en el 3.8 % ( 01) de los casos.It was carried out a descriptive, retrospective, transverse study; the sampling Unit was the patients with diagnose clinical of the Pronator Syndrome to Surgical Liberation in the Department of Orthopedics and Traumatology of the National Edgardo Rebagliati Martins Hospital between June of the 2003 and May of the 2004. It was also carried out the surgical liberation of the Transverse Ligament of the Carpo in all the patients with clinical square of compression of the Tunnel of the Carpo associated to the Pronator Syndrome. The statistical analysis was carried out with the program SPSS Vs. 11. 26 cases were selected, it was found that 73% (19) were bigger than 45 years and 69.2% (18) of female sex, 76.9% (20) were devoted to works that they were related to manual activities, 88.5% (23) were skillful and in 65.4% (17) the nervous atrapamiento was in the right extremity, in 84.6% (22) was carried out a liberation of the Syndrome of the Tunnel of the Carpo homolateral. Regarding the clinical maneuvers, 46.2% (12) presented pain in the point pronador, followed by 23.1% (06) supination against resistance, and 23.1% (06) pronation against resistance; the surgical discoveries indicated that in 42.3 % (11) was the aponeurosis bicipital, in 30.8 % (08) the band of the round pronador and in 19.2 % (05) cause tumorales those responsible for the compression, the postoperative complications, were hematoma 30.8 % (08), and infection in 3.8 % (01) of the cases.
Tesis de segunda especialidad
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Tárnok, Gábor. "Aerodynamická interakce dvou vozidel." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2010. http://www.nusl.cz/ntk/nusl-374732.
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Tárnok, Gábor. "Aerodynamická interakce dvou vozidel." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2011. http://www.nusl.cz/ntk/nusl-229895.
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Fryšták, Lukáš. "Aerodynamická optimalizace monopostu formule SAE." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2016. http://www.nusl.cz/ntk/nusl-254335.
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Tato práce se zabývá měřením aerodynamických charakteristik modelu závodního vozu Formula SAE v aerodynamickém tunelu, v měřítku 1:4. V první části je představen projekt Formula SAE a popsána role aerodynamiky v rámci této soutěže. Následuje přehled teoretického pozadí, které je relevantní k provedenému experimentu. Ve druhé části práce je popsán samotný experiment a prezentovány jeho výsledky. Součástí je návrh, výroba a kalibrace šestikomponentní tenzometrické váhy pro měření aerodynamického zatížení. Testy v aerodynamickém tunelu byly provedeny ve čtyřech konfiguracích, aby bylo možné určit vliv přítlačných křídel a podlahy s difuzorem na výsledné aerodynamické charakteristiky vozu.
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Bodié, Karen. "Nachweis von Apoptose am Magen- und Darmepithel des Pferdes mittels TUNEL-Methode und Immunhistochemie." Giessen : DVG Service, 2006. http://deposit.d-nb.de/cgi-bin/dokserv?idn=980609534.
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Ferreira, Laura Maria Canno 1977. "Analise experimental da curva de ligação poço-tunel em vertedor com emboque tipo tulipa." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/257857.
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Orientador: Ana Ines Borri GenovezTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Civil, Arquitetura e Urbanismo
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Resumo: Estruturas de queda com poços verticais podem ser utilizadas para drenagem urbana, dissipação de energia e como vertedores em barragens. No último caso, tem-se os vertedores com emboque tipo tulipa, formados por um perfil vertedor seguido de um poço vertical, que se liga a um túnel por meio de uma curva. A curva é um ponto crítico do dimensionamento do vertedor, pois, é onde o escoamento o deflete, causando elevadas pressões na sua parte convexa e subpressões na parte côncava. Este trabalho tem, como objetivo, o estudo experimental de um vertedor tipo tulipa, para definir uma melhor geometria para a curva de ligação poço-túnel. Um estudo experimental foi feito, empregando-se o modelo do vertedor de Paraitinga, em escala 1:51,02, utilizando novas geometrias para a curva, para avaliar as pressões e o arraste de ar. Pôde constatar-se a nfluência da geometria da curva, sendo que as novas curvas resultaram em um escoamento mais regular, acompanhando o traçado da curva, com pressões menores na parte convexa, além de uma maior vazão de afogamento do vertedor. Foram propostas equações para o cálculo da vazão de ar em função da concentração média do ar no escoamento. Através do estudo experimental comparativo entre as diferentes curvas se sugere o emprego da curva clotóide para ser usada na ligaçao poço-túnel
Abstract: Vertical dropshafts can be used in drainage systems, energy dissipator and as dam spillways. In this case, they are called morning glory, and consists of an inlet followed by a shaft which joins a tunnel through a bend. The bend is a critical point in spillway sizing, therefore the flow deflects in this region and causes high pressures in the convex part of the bend and negative pressures in the concavous part. This paper cares for the experimental study to define a better geometry for the shaft spillway vertical bend. An experimental study has been carried out, using the spillway model of Paraitinga dam, which scale was 1:51,02, using new bend geometries, to evaluate pressures flow and air entrainment. The influence of the bend geometry has been noticed, and the new curves resulted in a more regular flow, that followed the trace of the curve, with smaller pressures in the convex part, besides a higher flow to submerge the spillway. Equations have been proposed to calculate air entrainment flow as a function of the average concentration of air. Through the experimental study to compare the different curves, one suggest the use of use clotóide curve, as a bend shaft
Doutorado
Recursos Hidricos
Doutor em Engenharia Civil
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Hájek, Libor. "Realizace tunelu Radejčín - vybrané části stavebně technologického projektu." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2012. http://www.nusl.cz/ntk/nusl-225650.
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The issue of the thesis is a construction of the Radejčín highway tunnel. Project contains all of the stages of construction. The total budget and time schedule were made for complete structure. The technological instruction was made for secondary lining of the tunnel. There is also a plan of security instruction, the list of risks on construction site, suggestion of fixed job contract, the plan of controlls and exams for making invert and upper vault of secondary lining. Another part is the project of construction site with three different drawing of construction sites and text part. The list of machines and devices is for the complete construction. It was added also a chosen construction details and evaluation of geotechnical monitoring.
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Achterfeld, Christin [Verfasser], Stefan [Gutachter] Biesterfeld, and Monika [Gutachter] Hampl. "TV-bildanalytische Quantifizierung der TUNEL-Reaktion beim Mammakarzinom / Christin Achterfeld ; Gutachter: Stefan Biesterfeld, Monika Hampl." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2019. http://d-nb.info/1199102008/34.
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Reis, Maria Luisa Collucci da Costa. "Expressão da incerteza de medição associada a um ensaio aeronautico em tunel de vento subsonico." [s.n.], 2000. http://repositorio.unicamp.br/jspui/handle/REPOSIP/264293.
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Orientador: Olivio NovaskiTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecanica
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Resumo: Descreve-se um método para expressar a incerteza de medições associadas à realização de um ensaio aeronáutico no Túnel de Vento Subsônico TA-2, do Laboratório de Ensaios Aerodinâmicos - ASA - L, do Instituto de Aeronáutica e Espaço - IAE, do Centro Técnico Aeroespacial- CT A. O formalismo empregado é o recomendado por organizações metrológicas internacionais. É apresentado o procedimento de calibração da balança multicomponente que consiste em empregar o método de regressão multivariada. Os componentes da incerteza padrão, resultantes do ajuste de curva da cahoração, são posteriormente empregados para estimar as incertezas das forças e dos momentos atuantes no modelo que está sendo submetido ao ensaio aerodinânúco. É feita uma comparação entre o método aqui proposto e o utilizado atualmente no TA-2. São também apresentadas as metodologias de medição dos parâmetros do escoamento tais como pressão e temperatura estáticas, dinâmicas e totais, massa específica, velocidade, número de Reynolds e de Mach. As fontes de erro que contribuem para a incerteza da medição são identificadas, e as correções nas cargas aerodinâmicas devido à variação da pressão dinâmica, à posição do modelo e à tara e interferência são aplicadas. São apresentadas as relações funcionais das grandezas medidas, juntamente com as expressões de incerteza combinada, seguindo padronização adotada pela comunidade metrológica internacional. O intuito deste trabalho é contnouir para o desenvolvimento da ciência e das técnicas de medição aplicáveis a ensaios em túnel de vento subsônico. O estudo desenvolvido neste trabalho ajuda o profissional do laboratório a identificar os erros que comprometem os níveis de incerteza exigidos pelo experimento, e serve como um guia de qums procedimentos de medição devem ser adotados para atingir os resultados desejados
Abstract: This thesis describes a method to express the uncertainty of measurements associatec with canying out an aeronautical test in the Subsonic Wind Tunnel T A-2, of th( Aerodynamic Testing Laboratory ASA-L. at the Institute of Aeronautics and Space - IAE at the Aerospace Technical Center - CTA The methodology employed is tha recommended by the international metrological organizations. The calibration procedure o the multi-component balance which employs multivariate regression is presented here. Th standard uncertainty components resulting ftom the curve fit are later used to estima te tb uncertainty in forces and moments acting on the model which is being tested. A compariso is made between the method proposed here and that used in TA-2. This document aIs presents the measurement methodology of the flow parameters such as static. dynamic 8IJ total pressure and temperature. density. velocity. Reynolds and Mach numbers. The em sources which contnoutes to the uncertainty of the measurements are stated. TI corrections in the aerodynamic loads due to the variation of the dynamic pressure, to ti position of the model, and to the tare and interference are applied. The functior relationships of the measured quantities are presented, together with the combin expression ofthe uncertainty, in accordance with the standards adopted by the intemational metrological community. The object ofthis study is to contribute to the development ofthe science and the techniques of measurement which are applicable to subsonic wind tunnel tests. The study carried out in this thesis helps the laboratory staff to identify the errors which compromise the uncertainty leveI required by the experimento It serves as a guide as to which measwnent procedures should be adopted to achieve the required results
Doutorado
Materiais e Processos de Fabricação
Doutor em Engenharia Mecânica
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Barbin, Douglas Fernandes 1980. "Avaliação do processo de congelamento em tunel portatil com convecção forçada por exaustão e insuflação." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255757.
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Orientador: Vivaldo Silveira JuniorDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: A redução do tempo de congelamento de produtos alimentícios é um objetivo almejado, devido este processo ser caro e que envolve elevado gasto energético. Os produtos alimentícios são predominantemente congelados em túneis com convecção forçada por insuflação de ar; porém, é preferível realizar a exaustão de ar, ao invés de insuflação, pelo fato da exaustão promover uma circulação de ar mais uniforme ao redor do produto. Um túnel de ar forçado por exaustão é composto por um ventilador que circula de forma a retirar o ar, produzindo uma região de baixa pressão, onde se localiza o produto, buscando uniformizar a circulação do ar frio em contato com o mesmo. Desse modo, este trabalho se propôs à montagem experimental de um túnel ¿portátil¿ de congelamento por ar forçado, onde se pode estudar a utilização de insuflação e exaustão de ar. Este túnel foi construído e alocado no interior de uma câmara de armazenagem de produtos congelados, buscando melhorar a distribuição do ar, potencializando a troca térmica entre o ar e o produto a ser congelado. A montagem foi monitorada através de termopares para determinação das curvas de congelamento e eficiência do sistema. Durante o processo de congelamento, foi avaliada a transferência de calor através da montagem, comparando os processos de exaustão e insuflação e analisados os coeficientes de transferência de calor entre o ar de resfriamento e o produto em diferentes posições e camadas do palete, bem como a distribuição do ar de resfriamento em circulação ao redor do produto. Os resultados mostraram uma redução no tempo de congelamento das amostras com a utilização do túnel ¿portátil¿ em relação ao processo sem a utilização deste aparato dentro da câmara. O processo de exaustão apresentou uma redução de até quatro horas para o congelamento em relação à insuflação. Os valores de coeficiente de convecção foram maiores para a exaustão do que para a insuflação em todas as camadas da montagem, com exceção da superior, que recebia o ar diretamente do interior da câmara na insuflação. Um coeficiente de heterogeneidade foi proposto para avaliar a diferença de temperaturas no produto durante o congelamento. Estes valores, juntamente com a análise das temperaturas obtidas no processo, mostraram que a distribuição do ar, bem como a transferência de calor, ocorre de maneira mais homogênea no interior do palete na exaustão do que na insuflação
Abstract: The conditions necessary to keep the air temperature and movement at the product surface will determine the freezing process efficiency. Since the energy level could implement on the final cost, the reduction of the freezing process time was a major goal in the whole experiment. Food products are predominantly frozen in air blast freezing tunnels. Therefore, exhausting air is preferable than blowing it through, since it minimizes air short-circuiting and results in more uniform cooling. To produce an homogeneous refrigeration, the main configuration to be determined for pallets storing plastic packages in boxes are the package distribution and air orientation. The main components of an exhausting forced-air tunnel are a fan that causes the equipment inside air evacuation creating a low pressure region. The product is arranged on this spot creating uniform distribution of the cold air inside the equipment, around the product. Therefore, the objective of this work is to build an experimental portable forced-air freezing tunnel, and work on comparative studies with air exhausting and blowing. The tunnel was built and placed inside a freezing product storage chamber, and the objective was to improve the air circulation and the thermal distribution between the product and cold air, for a sample batch left inside the chamber. The batch was monitored using thermocouples for freezing variation and system efficiency graphic determination. It was also provided the heat transfer analysis, comparing the exhausting and blowing process, and the heat transfer coefficients of the cold air and the product as well as the air distribution around the product. The results have shown reduction of the freezing time of the samples when the portable tunnel was used comparing without the tunnel tests (reference). The air evacuation process reduced up to four hours with comparison to the blowing system the freezing process. Convective coefficient results were higher for air evacuation than air blowing in every part of the batch, except for the upper layer of products were the cold air of the chamber was directly in contact with the product. These results, with the temperature analysis obtained, indicated that the air distribution occurs more uniformly around the products in the exhausting process than blowing system, as well as the heat transfer
Mestrado
Mestre em Engenharia de Alimentos
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Kaleff, Paulo Roland 1976. "Utilização de tecnica de incisão transversa minima no tratamento da sindrome do tunel do carpo." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308844.
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Orientador: Donizeti Cesar HonoratoDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Propósito:Avaliação de técnica de incisão transversa limitada no tratamento da síndrome do túnel do carpo, quanto à segurança cirúrgica e efetividade na abertura do Retináculo Flexor(RF). Método: Estudo prospectivo de Trinta procedimentos realizados em vinte e oito pacientes submetidos à técnica com incisão transversa mínima. A segurança da técnica e a abertura total do RF foram avaliadas através de questionário baseado em observações clinicas e inspeção endoscópica, respectivamente. Resultados: Não foram observadas complicações maiores. Um único paciente apresentou neuropraxia de N. interdigital. Dois pacientes apresentaram hematoma local pequeno. Em dois dos cinco primeiros casos, durante a inspeção endoscópica, observou-se abertura incompleta do RF, sendo necessária abertura complementar. Todos os pacientes apresentaram melhora do quadro clínico de dor noturna e parestesias. Conclusão: A técnica foi executada com segurança no grupo analisado, sem a ocorrência de complicações graves, e com abertura do RF na quase totalidade dos casos. Uma avaliação de longo prazo e com um número maior de pacientes é necessária.
Abstract: Purpose: To evaluate the application of a limited transverse incision technique to treat the Carpal tunnel syndrome, with concern to its safety and efficacy in the opening of the Flexor Retinaculum (FR). Method: A prospective analysis of thirty FR release procedures performed on twenty-eight patients subjected to the proposed incision technique. Safety and total opening of the FR were evaluated through a questionnaire and an endoscopic inspection respectively. Results: No major complications were observed. Two cases presented small local hematoma. One patient presented with transient neuropraxia of digital branch. In two of the first five cases, incomplete FR opening was identified during endoscopic revision with need of complementary opening. All patients showed symptom relief. Conclusion: The technique was safely performed on the prospection group, no major complications were detected and the opening of FR was observed in the majority of the patients. Long term results and with a larger series of patients should be evaluated in further studies.
Mestrado
Neurologia
Mestre em Ciências Médicas
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Semotam, Zbyněk. "Analýza vlivu procesu ochlazování na kvalitu proteického gelu." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2009. http://www.nusl.cz/ntk/nusl-228731.
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This diploma thesis deals to possibilities lead chilling process with material loss of product and divine to possibilities lead texture final product with chilling process. This work will be base of project for realization chilling tunnel to flow line of proteinic gel.
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INAGAKI-TACHIBANA, EIKO, TAKAMITSU TSUKAHARA, KAZUHIKO KAJI, RYOJI EGUCHI, HIROAKI KANAZAWA, HISAYOSHI HAYASHI, and YUICHI SUZUKI. "INVOLVEMENT OF DNA FRAGMENTATION OF ENTEROCYTES IN MUCOSAL INJURY TO A MOUSE JEJUNUM INCUBATED IN USSING CHAMBERS." Nagoya University School of Medicine, 2009. http://hdl.handle.net/2237/11332.
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Eshaque, Bithi. "Characterization of Eukaryotic Translation Initiation Factor 5A isoforms (eIF-5A1 & eIF-5A2) using human cell lines as a model system." Thesis, University of Waterloo, 2006. http://hdl.handle.net/10012/1218.
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Eukaryotic translation initiation factor 5A (eIF-5A) is the only known cellular protein that contains the post-translationally derived amino acid, hypusine. Initially, eIF-5A was named as a translation initiation factor because of its capability to stimulate the formation of methionyl-puromycin, which mimics the first peptide bond formation during protein synthesis, under in vitro conditions. Subsequently, however, this proposed function of eIF-5A has been questioned because a similar effect on translation was not observed in situ. Moreover, eIF-5A appears not to be required for general protein synthesis. Rather, there is evidence that it facilitates the translation of specific subsets of mRNAs required for cell proliferation as well as apoptosis. There are two isoforms of eIF-5A in the human genome which have designated eIF-5A1 and eIF-5A2. The objective of the present study was to gain an increased understanding of the roles of eIF-5A1 and eIF-5A2 during apoptosis and cell proliferation using human cell lines as a model system. Apoptosis was induced by treating the cells with Actinomycin D or sodium nitroprusside (SNP), which initiate programmed cell death by different mechanisms. It was observed for both normal and cancer cells that eIF-5A1 protein is up-regulated during apoptosis induced by Actinomycin D or SNP, whereas eIF-5A1 mRNA is constitutively expressed and does not change in abundance during this treatment. The up regulation of eIF-5A1 protein levels in the absence of a corresponding up-regulation in eIF-5A1 mRNA suggests that eIF-5A1 may be post-transcriptionally regulated. Moreover, eIF-5A1 protein up-regulation was stronger in normal cells than in cancer cells. By contrast, eIF-5A2 protein was below detection levels during apoptosis in both normal and cancer cells, although the corresponding transcript was detectable by semi-quantitative RT-PCR. This is attributable to inefficient translation of eIF-5A2 mRNA.
The effects of eIF-5A1 and eIF-5A2 on cell proliferation were examined by modulating the levels of serum in cultures of UACC-1598 cells, which are ovarian cancer cells that express high levels of both isoforms of eIF-5A. Serum starvation, which induces cell cycle arrest and ensuing apoptosis, followed by the re-addition of serum had no effect on the transcript levels of either eIF-5A1 or eIF-5A2. However, eIF-5A1 and eIF-5A2 proteins were both up-regulated within 24 hours of the initiation of serum starvation, and this coincided temporally with the onset of apoptosis as measured by TUNEL and a subsequent decline in viable cells.
The data indicate that eIF-5A1 and eIF-5A2 are both post-transcriptionally regulated and that they have functionally redundant roles in apoptosis.
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ARAIZA, ORTIZ FRANCISCO JAVIER, and FALFAN JULIO CESAR BALCAZAR. "INCIDENCIA DEL SIÍNDROME DEL TÚNEL DEL CARPO EN CIRUJANOS DENTISTAS DE LA UNIDAD DE ESPECIALIDADES ODONTOLÓGICAS DE LA SECRETARÍA DE LA DEFENSA NACIONAL, 2017." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2018. http://hdl.handle.net/20.500.11799/99311.
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Tesis de Licenciatura en Terapia FísicaEl STC se produce como resultado de la presión del nervio mediano bajo la aponeurosis flexora. Aí mismo, el edema o la tenosinovitis de los tendones flexores pueden provocar un mayor volumen en el túnel carpiano desencadenando los signos y síntomas que caracterizan el síndrome. En general esta patología es una condición de gran interés ya que al ser detectado en forma oportuna influirá en un mejor desempeño de los Cirujanos Dentistas al realizar prácticas de su especialidad. Se considera importante el estudio de esta patología, debido a que dentro del ámbito laboral el síndrome del túnel del carpo puede estar causado y agravado por la ocupación. La mayoría de los pacientes que lo presentan relacionan el trabajo con la lesión y en este caso, los Cirujanos Dentistas militares de la Unidad de Especialidades Odontológicas de la Secretaría de la Defensa Nacional (SEDENA) se ven expuestos a una aumentada carga de trabajo debido a la demanda de atención odontológica que se presenta en esta institución, dentro de la cual realizan actividades con las manos en forma repetitiva, de constante presión y que se encuentran sometidas a vibraciones de alta y mediana intensidad emitidas por sus instrumentos de trabajo, las cuales se consideran un factor importante para establecer este trastorno musculoesquelético. Al realizar un diagnóstico fisioterapéutico, es preciso evaluar el dolor, la sensibilidad, la funcionalidad, la afectación nerviosa y el estado general del paciente, para ello, se emplearon maniobras de provocación de la sintomatología como los test de Phalen, Tinel y Flick y se aplicó un cuestionario. También se requiere conocer las exposiciones ergonómicas adversas tanto dentro como fuera del trabajo. El STC. Tiende a disminuir la calidad de vida del Cirujano Dentista la cual vino en detrimento en la atención de los pacientes, debido a la sintomatología aguda o crónica que limito a este personal en la capacidad de realizar en forma óptima sus actividades profesionales y de la vida diaria. En su actividad profesional, el fisioterapeuta debe promover medidas de prevención que incluyen la educación sanitaria, la educación ergonómica sobre los factores de riesgo. El reconocimiento temprano del STC, nos dará oportunidad de sugerir dentro de las actividades diarias del Cirujano Dentista, ejercicios aeróbicos, ejercicios posturales, ejercicios de elongación y de relajación preventivos. De esta situación surgió la necesidad de saber cuál es la incidencia del Síndrome del Túnel del carpo en Cirujanos Dentistas de la Unidad de Especialidades Odontológicas de la Secretaría de la Defensa Nacional durante los meses de Agosto a Noviembre del 2017 y así en su momento encontrar las condiciones de plantear un tratamiento preventivo y correctivo para estos pacientes.
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Vuk, Vujasinović. "Prinos i kvalitet mladog krompira u zavisnosti od agrotehničkih mera i agroekoloških uslova proizvodnje." Phd thesis, Univerzitet u Novom Sadu, Poljoprivredni fakultet u Novom Sadu, 2016. https://www.cris.uns.ac.rs/record.jsf?recordId=101166&source=NDLTD&language=en.
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Proizvodnja mladog krompira u Srbiji je značajna zbog obezbeđenja ove izuzetno cenjene i delikatesne namirnice u periodu od aprila do juna kada je u ponudi na tržištu malo drugog svežeg povrća. Osim toga cena mladog krompira je uglavnom visoka, pa je značaj i po osnovu profitabilnosti ove kulture velik.U periodu 2004-2006. godine ispitivan je efekat malčovanja polietilenskom folijom ineposrednog pokrivanja biljaka sa i bez noseće konstrukcije, kao i kombinacija ovih specifičnih agrotehničkih mera na ranostasnost, prinos i komponente prinosa i kvalitet mladog krompira kod ranih sorti Cleopatra i Riviera. U cilju rešavanja postavljenog zadatka sprovedeno je istraživanje, na lokalitetu južno bačkog okruga (Begeč), postavljen je dvofaktorijalni ogled po split plot planu u pet ponavljanja.Ispitivane agrotehničke mere i njihove kombinacije značajno su uticale na ranostasnost i prinos mladog krompira. Formiranje tržišnih krtola (krtole veće od 28 mm) bilo je za 10-15 dana ranije u odnosu na kontrolnu (nepokrivenu) varijantu. Najveći prinos u trogodišnjem istraživanju postignut je na varijanti agrotekstil+niski tunel (40,07 t/ha). U odnosu na kontrolnu varijantu, prinos je bio veći za 19% (u prvoj godini za 22%, drugoj za 16%, dok je u trećoj godini prinos bio veći za 18%).Najveći broj krtola mladog krompira, u trogodišnjem istraživanju, ostvaren je nakontrolnoj varijanti (9,43), stim što je udeo tržišnih krtola (krtole veće od 28 mm) na kontroli iznosio 89% naspram 95% ostvaren na pokrivenim varijantama. Riviera je ostvarila veći broj krtola po biljci u odnosu na Cleopatru.Ispitivane agrotehničke mere uticale su na povećanje mase krtola po biljci. Najveća masa krtola po biljci u trogodišnjem ogledu ostvaren je na tretmanima agrotekstil+niski tunel (853,17 g) i malčovanje+agrotekstil+niski tunel (850,17 g), dok je najmanja masa zabeležena na kontrolnoj varijanti (640 g). Sorta Riviera je ostvarila značajno veću masu krtola u odnosu na Cleopatru.Ispitivani tretmani su ostvarili veću prosečnu masu jedne krtole za 52% u odnosu nakontrolnu varijantu.Ispitivani tretmani značajno su uticali i na kvalitet mladog krompira; odnosno napovećanje sadržaja suve materije, skroba, vitamina C, celuloze i pepela u krtolama, a nasmanjenje sadržaja šećera i nitrata.Production of early potato in Republic of Serbia is important because it provides thishighly valued delicates food during the period from April to June, when there is low offer of otherfresh vegetables on the market. Besides, price of early potato is usually high so it is important asprofitable crop.During the period 2004-2006 effect of mulching with polyethylene foil and directcoverage of plants with agrotextile and low tunnel, as well as combinations of these specificagricultural measures on earliness, yield and yield components and quality of two early potatovarieties Cleopatra and Riviera was examined. In order to achieve given objective research wasconducted at the site in South Bačka District (Begeč), it set up the two-factorial experiment atsplit plot plan in five replications.The tested of agricultural measures and their combinations significantly influencedearliness and yield of early potato. The forming of market tubers (tubers larger than 28 mm) was10-15 days earlier than on the control variant (bare soil and uncover plants). The highest yield ofthe three-year study has been made on the variant combination of agrotextile and low tunnel(40,07 t/ha). Compared to the control variant, the yield was increased by 19% (in the first yearincrease of 22%, second year increase was 16%, while in the third year yield increase was 18%).The largest number of tubers of early potatoes, in three-year study, has been on thecontrol variant (9,43), but the share of market tubers (tubers larger than 28 mm) on the controlvariant was 89% versus 95% achieved in covered variants. Variety Riviera has achieved greaternumber of tubers per plant compared to variety Cleopatra.The tested agricultural measures affected mass increase of tubers per plant. Themaximum weight of tubers per plant in the three-year experiment was realized in the treatmentcombination of agrotextile and low tunnel (853,17 g) and combination of mulching, agrotextileand low tunnel (850,17 g), while the lowest mass was recorded in the control variant (640 g).Variety Riviera has achieved significantly higher tuber weight relative to Cleopatra.The tested treatments have also achieved a higher average weight of a tuber of 52%compared to the control variant.The tested treatments significantly influenced quality of early potato; i.e. the increase thecontent of dry matter, starch, vitamin C, cellulose and ash in the tubers and on reduction of sugarand nitrate content.
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Oliveira, Rone Batista de [UNESP]. "Caracterização funcional de adjuvantes em soluções aquosas." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/101668.
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A alta demanda por agrotóxicos pelos sistemas de produção agrícola, aliada ao possível uso inadequado desses produtos, torna a deriva um dos maiores problemas potenciais da agricultura atual, e a escolha correta de adjuvantes pode ser uma das alternativas para minimizar os impactos negativos causados pela deriva nas pulverizações. Este trabalho foi desenvolvido com o objetivo de avaliar o efeito proporcionado por diferentes grupos de adjuvantes nas propriedades físicas e químicas das soluções, no espectro de gotas, bem como no potencial do risco de deriva quantificado em túnel de vento, como forma de auxiliar na escolha correta desses produtos para melhor utilização nas aplicações de agrotóxicos. Foram selecionados 18 adjuvantes comumente utilizados em misturas com agrotóxicos em pulverizações agrícolas, avaliando-se as propriedades físicas e químicas dos mesmos em soluções aquosas (viscosidade, densidade, tensão superficial e condutividade elétrica), o espectro de gotas e o potencial de deriva medido em túnel de vento. O experimento foi composto de 33 tratamentos, tendo a água como testemunha adicional, resultantes da diluição dos adjuvantes em diferentes concentrações, com três repetições. Para os ensaios em túnel de vento e análise de espectro de gotas foi utilizada uma ponta de pulverização XR8003 VK na pressão de 200 kPa, gerando um padrão de gotas médias. A deriva foi coletada no túnel de vento com fluxo de ar na velocidade de 2 m s-1. O corante Azul Brilhante a 0,6% (m v-1) foi utilizado como marcador para todas as soluções pulverizadas visando a quantificação da deriva por espectrofotometria. Os resultados mostraram que a adição de adjuvantes alterou as propriedades físicas e químicas das soluções aquosas em diferentes magnitudes, dependendo da concentração utilizada. Os surfatantes organosilicones...
Due to the high demand for pesticide by the agricultural systems and the possible inadequate use of the products, drift became one of the biggest concerns of the modern agriculture, and the correct use of adjuvants may be one of the possible alternatives to minimize the negative impact of spray operations. This study was developed to evaluate the effect of different adjuvants on physical and chemical properties of spray solutions, droplets spectra as well as drift potential measured in wind tunnel, aiming the correct choice of adjuvants to improve efficiency in pesticide application. For this purpose 18 commercially available agricultural spray adjuvants were selected including products usually mixed up with pesticides in the sprayer tank. The evaluation included physical and chemical properties of spray solutions (viscosity, density, surface tension and electric conductivity), droplets spectra and drift risk potential measured in wind tunnel. The experiment was set up with 33 treatments (solutions) obtained from 18 adjuvants used in different concentrations with 3 replications, including pure water as a standard. For wind tunnel tests and droplet spectra analysis it was used a Teejet XR8003 flat fan nozzles at 200 kPa (medium droplets) and all solutions were mixed up with a food color dye (Blue FDC) at 0,6% m v-1 for spectrophotometry analysis of drift deposits on the wind tunnel. The results showed that the addition of adjuvants changed physical and chemical properties of spray solutions in different magnitudes according to the product concentration. The organosilicon surfactants (Silwet 0.1% e 0.2% and BreakThru 0,1%) had the lowest values for surface tension, while the drift retardant based polymers (Define 0,06% e 0,12%) had the highest values for viscosity and density. There was high correlation between the Volume Median Diameter (VMD) and percentage of droplets smaller... (Completo abstract click electronic access below)
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Gomboš, Michal. "Vodojemy - Brno, Žlutý kopec." Master's thesis, Vysoké učení technické v Brně. Fakulta architektury, 2021. http://www.nusl.cz/ntk/nusl-451226.
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After successfully avoiding of the destructive scenario with the demolition of reservoirs and replacing them with parking house, the second question arises. How to stimulate the people's interest in these underground jewels, the interest that would confirm the decision to save reservoirs. One of the possible ways, in which I see a purpose and the one that I decided to chose is to make the reservoirs accessible alongside the pathway and let the natural curiosity of human to do the rest.
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Kaderka, Jan. "Návrh dálničního tunelu Polana." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2015. http://www.nusl.cz/ntk/nusl-227839.
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The subject topic of this thesis is to establish a suitable technique of tunnel construction using New Austrian Tunnelling Method with the help of mathematical modeling. Based on the mathematical modelling output the dimensioning primary moulding model was executed.
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Souza, Rafael Lopes de. "Desenvolvimento de um microscópio de varredura por tunelamento operado em ultra alto vácuo." CNEN - Centro de Desenvolvimento da Tecnologia Nuclear, Belo Horizonte, 2013. http://www.bdtd.cdtn.br//tde_busca/arquivo.php?codArquivo=299.
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Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorInventado no início dos anos 1980, o STM evoluiu para uma importante ferramenta na investigação das propriedades de superfícies e interfaces, com aplicações em várias áreas da ciência além da Física, como Ciências de Materiais e Química. O presente trabalho trata da microscopia de tunelamento, que se baseia no fenômeno do tunelamento quântico dos elétrons. No STM, a distância entre ponta e a amostra é reduzida até que as funções de onda dos elétrons na ponta e na superfície da amostra se sobreponham. Nessa situação, observa-se o fenômeno do tunelamento quântico de elétrons através da barreira formada entre os dois eletrodos (ponta e amostra). Como o valor da corrente de tunelamento é fortemente dependente da distância ponta-amostra, um microscópio STM pode ser utilizado para mapear a morfologia da superfície da amostra com alta resolução espacial. Além disso, outra importante capacidade do STM é a possibilidade de atuar no modo espectroscópico (STS). Por vezes, o estudo detalhado das propriedades de um sistema requer o uso de métodos não convencionais de microscopia STM. Um exemplo é o estudo do magnetismo de nanoestruturas por microscopia de tunelamento com resolução em spin (SP-STM). A implementação destes métodos não convencionais normalmente exigem recursos experimentais específicos, nem sempre disponíveis em equipamentos comercias. A versatilidade no controle das características funcionais do equipamento foi a principal razão que nos motivou a construir o microscópio STM. No primeiro capitulo faremos uma introdução geral ao tema da dissertação. Uma breve introdução ao método de STM será dada no capítulo 2, incluindo aspectos fundamentais do tunelamento quântico, bem como sua aplicação técngeralica. O capítulo 3 descreve o estudo das propriedades eletrônicas e magnéticas do grafeno preparado sobre a superfície vicinal Ni(977). O magnetismo observado na camada de grafeno induzido por um substrato ferromagnético é de grande interessante para o desenvolvimento de dispositivos spintrônicos. Relatamos a investigação das propriedades de uma monocamada de grafeno preparada sobre de Ni(977) por CVD, utilizando a microscopia de varredura por tunelamento (STM), dicroísmo circular magnético de raios-x (XMCD) e espectroscopia de fotoelétrons (XPS). No capítulo 4, apresentamos o detalhamento do projeto de construção de um STM para operar em ambiente de ultra alto vácuo (UHV). Características do STM como isolamento de vibração, desenho mecânico, sistema de varredura e processo de preparação de pontas são discutidos. Por fim, analisamos os resultados dos testes de operação no microscópio STM, dificuldades observadas ao longo do projeto e possíveis melhorias.
Invented in the early 1980s, STM has evolved into a standard tool to investigate the properties of surfaces and interfaces, with applications in various research fields, such as physics, material sciences and chemistry. The present work deals with scanning tunnelling microscopy, which refers to the quantum phenomenon of electron tunneling through a potential barrier. The distance between a conductive probe tip and sample is reduced until the electron wave functions of tip and sample surface have significant overlap, and electrons can tunnel through the vacuum barrier. As the so-detected tunneling current is strongly distance-dependant, it can be used to map the morphology of the sample surface with a resolution which goes far beyond the actual meaning of the term microscopy. Besides its unique spatial resolution, one strength of the STM is the possibility to perform local electronic spectroscopy. STM has evolved into a standard tool to investigate the properties of surfaces and interfaces, with applications in various research fields besides physics, such as material sciences, chemistry, or biology. Nevertheless, the detailed study of materials properties requires the use of non-conventional methods of STM microscopy. One example is the study of magnetism in nanostructures by spin polarized scanning tunneling microscope (Sp-STM). The implementation of such unconventional methods typically requires specific experimental features, not always available in commercial equipment. This versatility in controlling the functional characteristics of the equipment was one of the main reasons that motivated us to design and build our own STM microscope. A general introduction to the dissertation theme is presented in chapter 1. In chapter 2, a brief discussion about the method of STM is developed, including theoretical aspects on quantum tunneling. In chapter 3, the experimental study of electronic and magnetic properties of the graphene/Ni(977) is shown. The recent observation of magnetism in graphene layers induced by a ferromagnetic substrate is a very interesting issue and can impact the design of new carbon-based spintronic devices. Here we report on the investigation of the electronic and magnetic properties of the graphene/Ni(977) by using scanning tunneling microscope (STM), x-ray magnetic circular dichroism (XMCD) and x-ray photoelectron spectroscopy (XPS). In chapter 4, it was described the project and construction of a homemade STM operated in ultrahigh vacuum (UHV) condition. We discuss the details of main STM-UHV components: main and sample preparation chambers, STM head, tip scanner, and sample holder design. Characteristics of the mechanical and electronic design, vibration isolating system, tip and sample preparation are discussed. Finally, we report the results of testing experiments as well as discuss the encountered difficulties and some possible solutions.
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Bodié, Karen [Verfasser]. "Nachweis von Apoptose am Magen- und Darmepithel des Pferdes mittels TUNEL-Methode und Immunhistochemie / eingereicht von Karen Bodié." Gießen : DVG-Service, 2006. http://d-nb.info/980609534/34.
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Tagliavini, João Virgilio. "Garotos no tunel : um estudo sobre a imposição da vocação sacerdotal e o processo de condicionamento nos seminarios." [s.n.], 1990. http://repositorio.unicamp.br/jspui/handle/REPOSIP/279389.
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Orientador : Sergio Miceli Pessoa de BarrosDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas
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Resumo: Não informado
Abstract: Not informed
Mestrado
Mestre em Ciências Sociais
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Salaya, Sánchez Germán Ignacio. "Estudio experimental de la estela de un aerogenerador de tres aspas." Tesis, Universidad de Chile, 2013. http://www.repositorio.uchile.cl/handle/2250/116011.
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Ingeniero Civil MecánicoLa creciente utilización de sistemas de generación de energía renovable ha provocado un aumento importante en la investigación y desarrollo de estas tecnologías. Particularmente, el campo de la generación mediante energía eólica es uno de los más importantes hoy en día. Un factor determinante en la capacidad de generación a través de energía eólica es la eficiencia de las turbinas. Es común en estas máquinas encontrar eficiencias en torno al 45\%, Entre los factores que determinan la eficiencia están los aspectos geométricos y aerodinámicos de la turbina, y la estela turbulenta generada por las palas y el rotor. Esta ultima en particular es determinante tanto en la eficiencia individual de aerogeneradores como en la eficiencia global en el caso de parcelas eólicas, debido a la interferencia entre distintas turbinas. Se sabe que la gran mayoría de las pérdidas de eficiencia de las turbinas eólicas se deben a turbulencias generadas por las palas del rotor, por lo que la minimización de estas es el objetivo principal de los diseñadores hoy en día. Estos aspectos motivaron este trabajo de título, en el cual estudiamos experimentalmente el funcionamiento de una turbina eólica al interior de un túnel de viento mediante anemometría de hilo caliente. Se diseñó y construyó un modelo a escala de un aerogenerador y se instaló dentro del túnel de viento del laboratorio LEAF-NL, de la Universidad de Chile Se midió la potencia de la turbina como función de la velocidad de flujo del túnel de viento en un rango de velocidades de flujo de entre 0.5 y 3 [m/s] (TSR o tip speed ratio de 1.1 a 5.3), lo que entrega como resultado la curva característica de los aerogeneradores (curva S). A través un sistema de posicionamiento motorizado en 2D se midió el campo de velocidades en distintos planos transversales de la estela. Para esto se utilizó un anemómetro de hilo caliente operado de manera síncrona con la posición angular instantánea del rotor de la turbina, con el objetivo de realizar una detección coherente de la velocidad en la estela con la posición angular del rotor. De esta manera se obtuvo el campo de velocidades medio y fluctuante asociado a la posición de las palas de aerogenerador. La medición sincronizada de los promedios temporales de velocidad permite identificar la influencia de cada de cada pala en la estela y además identificar la distribución de intensidad turbulenta. Finalmente se generaron mapas de velocidad en distintos planos de corte transversal a la estela, con lo que adicionalmente se logró evaluar la expansión de la estela y poder obtener un parámetro de razón de áreas, dicho parámetro permite calcular con éxito los coeficientes de potencia y empuje (Cp y Ct), los cuales coinciden con los valores reales medidos.
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Bilyeu, David Lawrence. "Numerical Simulation of Chemical Reactions Inside a Shock-Tube by the Space-Time Conservation Element and Solution Element Method." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1213363652.
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Silva, Flávio Vasconcelos da 1971. "Avaliação do comportamento experimental do sistema de refrigeração de um tunel de congelamento com R-502 e R-408A." [s.n.], 1998. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255752.
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Orientador: Vivaldo Silveira JuniorDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Com a implantação das restrições adotadas a partir do Protocolo de Montreal, torna-se importante a avaliação experimental do desempenho de novos fluidos refrigerantes. O R-502 (mistura azeotrópica HCFC-22 / CFC-115; 48,8% / 51,2%) é extensivamente utilizado como fluido refrigerante em equipamentos de refrigeração a baixas temperaturas. Já o R-408A (mistura quase azeotrópica composta de HCFC-22 / HFC-125 / HFC-143a; 47% / 7% / 46%) pode apresentar vantagens como substituto do R-502 em sistemas em funcionamento, visto seu comportamento termodinâmico, baixa toxidade e não inflamabilidade. Os ensaios foram conduzidos em um túnel de congelamento de alimentos instrumentado com sensores de velocidade de ar, temperatura e pressão, cujo sistema frigorífico operou sob diferentes condições em regime estacionário. O ciclo de refrigeração, capacidade frigorífica, comportamento de troca térmica na dissipação de calor, coeficiente de desempenho do sistema, potência de compressão, potência consumida, desempenho global do sistema de refrigeração e fatores de sensibilidade das variáveis manipuladas foram avaliados entre os dois refrigerantes, evitando-se substituições ou modificações físicas no sistema. Os resultados obtidos após os testes de desempenho do R-502 e R-408A permitiram uma avaliação comparativa das condições de operação do sistema frigorífico através de modelos empíricos. O comportamento do R-408A no sistema apresentou um ganho de 20 a 30% no coeficiente de desempenho útil e 29,5% no coeficiente de desempenho total, nas condições de referência. O consumo de energia elétrica do R-408A superou em até 7% o consumo do R-502, porém apresentando uma maior capacidade frigorífica, em torno de 30%, em todos os ensaios. O calor de compressão apresentou um aumento de 9,5%. Sob algumas condições houve uma redução de 0,5% na temperatura do ar de insuflamento utilizando-se o R-408A. No sistema estudado, a temperatura de descarga do R-408A apresentou incremento de até 1% e 0,8% operando com menor e maior vazão de água no condensador, respectivamente. Ocorreu uma redução de 14 a 20% na razão de compressão do R-408A
Abstract: An experimental evaluation of the new cooling fluid performance is important because of the introductions of the adopted restrictions of the Montreal Protocol. R-502 (azeotropic blend of HCFC-22 / CFC-115; 48,8% / 51,2%) is extensively used as a cooling fluid in low-temperature refrigeration equipments. R-408A (near-azeotropic blend of HCFC-22 / HFC-125 / HFC-143a; 47% / 7% / 46%) can show advantages as a substitute for R-502 in operating systems, due to its thermodynamic behaviour, low toxicity and no flame propagation. Tests were conducted in a food freezing tunnel, instrumented with air velocity, temperature and pressure sensors, which operated under different steady-state conditions. The refrigeration cycle, cooling capacity, thermal exchange behaviour, coefficient of performance, compression power, electrical demand, global performance of the refrigeration system and sensitivity factors of the handled variables were evaluated for two refrigerants, avoiding alterations or physical modifications in the system. The results after the R-502 and R-408A performance tests made it possible to comparatively evaluate the refrigeration system operation conditions through empirical models. The R-408A performance in system presented a gain of 20 to 30% in the useful coefficient of performance and 29,5% in the total coefficient of performance, in the reference conditions. The electrical energy consumption when using the R-408A overcame up to 7% the R-502 electrical consumption, neverthless it presented a better refrigeration capacity, around 30%, for all the tests. The compression heat presented an increase of 9,5%. Under some conditions there was a reduction of 0,5% in the air temperature inside the tunnel, while using the R-408A. In the studied system, the R-408A discharge temperature presented an increment of 1% and 0,8% when operating with lower and higher water flow in the condenser, respectively. There was a reduction of 14-20% in the R-408A pressure ratio
Mestrado
Mestre em Engenharia de Alimentos
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Clouet-Leské, Anne-Claire Jean Miguel. "Facteurs environnementaux et spermatogénèse application de la technique TUNEL pour l'évaluation du taux de fragmentation de l'ADN du spermatozoïde /." [S.l.] : [s.n.], 2006. http://theses.univ-nantes.fr/thesemed/PHclouet.pdf.
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Bastl, Jiří. "Návrh ražby a primárního ostění Dřevnovického tunelu na stavbě vysokorychlostního železničního spojení Brno – Přerov." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2019. http://www.nusl.cz/ntk/nusl-392301.
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This master thesis deals with a design of a Drevnovice tunnel primary lining at planned high speed rail corridor connecting Brno and Přerov. The whole design as well as defining of the most suitable tunnel excavation method are described. Thesis also contains a breakdown of the excavation, construction technology and the scheduling of geotechnical monitoring procedures. The Plaxis 2D program, based on the finite element method, was used to create numerical models of two cross sections of the Dřevnovice tunnel. Based on mathematical modeling outputs, a static calculation of the primary lining was calculated.
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Dvořák, Rostislav. "Stanovení obsahu platiny na území města Brna." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-217073.
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This thesis is focused on the determination of platinum in samples of road dust and soil by atomic absorption spectrometry. The theoretical part describes the basic physical and chemical properties of platinum, platinum function in automotive converters and its behavior in the environment. In the experimental section summarizes the methods used to decompositions samples, concentration of the analyte and the main results of the measurements.
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De, la Fuente Mata Manuel. "Tunneling under squeezing conditions : Effect of the excavation method." Thesis, Paris Est, 2018. http://www.theses.fr/2018PESC1071/document.
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L’excavation d’un tunnel profond dans des terrains poussants pose des difficultés particulières de conception et d’exécution. Ce type de terrain est caractérisé par des fortes convergences en paroi du tunnel de nature différée et souvent anisotrope. Le comportement d’un tunnel excavé en terrain poussant est très influencé par la technique d’excavation utilisée. Le cas d’étude du tunnel routier du Fréjus et de sa galerie de sécurité permet d’illustrer ce phénomène. Il s’agit de deux tunnels parallèles qui montrent une configuration très intéressante étant donné qu’ils traversent des conditions géotechniques similaires et qu’ils sont creusés avec des techniques d’excavation différentes : le tunnel routier a été creusé par méthode conventionnelle à l’explosif tandis que la galerie de sécurité a été creusée avec un tunnelier à bouclier simple. Les mesures d’auscultation réalisées pendant l’excavation des deux tunnels ont été analysées et comparées. Des modélisations numériques pour simuler la réponse des deux tunnels ont été développées avec le logiciel Flac3D. Le comportement du terrain est simulé avec un modèle visco-elasto-plastique et anisotrope. L’anisotropie liée à la schistosité du terrain est introduite dans le modèle par la présence de plans de faiblesse d’orientation donnée (ubiquitous joint model) insérés dans une matrice rocheuse caractérisée par un comportement visco-elasto-plastique isotrope. Une rétro-analyse a été réalisée sur les mesures de convergence obtenues lors du creusement du tunnel routier du Fréjus. Le comportement du terrain identifié dans le tunnel routier est ensuite extrapolé pour prédire la réponse de la galerie de sécurité. L’objectif est de reproduire l’état des contraintes observé dans les voussoirs de la galerie de sécurité et d’extrapoler les sollicitations à long terme. L’influence que la technique d’excavation, en particulier sur le comportement différé du terrain a été prise en compte dans les simulations numériques. On a mis en évidence que les déformations différées du terrain sont réduites lorsque l’excavation est réalisée au tunnelier.Par ailleurs, une synthèse critique de la méthode convergence-confinement et de ses variantes a été réalisée. Une discussion a été menée sur l’applicabilité des méthodes convergence-confinent quand elles sont utilisées pour le dimensionnement des tunnels circulaires excavés en section pleine avec l’installation d’un soutènement raide près du front d’excavation comme c’est le cas lors d’une excavation au tunnelier. Dans ce contexte, un ensemble de formules empiriques sont proposées. Elles permettent d’obtenir avec une bonne précision l’état d’équilibre entre le terrain et le soutènement et peuvent être utilisées dans la phase de pré-dimensionnement des ouvragesDuring the excavation of deep tunnels, squeezing ground conditions are often encountered. The squeezing behavior of the ground is characterized by large time-dependent and usually anisotropic convergences that take place at the tunnel wall. The technique of excavation has a strong influence on the tunnel response when it is excavated under squeezing conditions. This phenomenon is illustrated throughout the case study of the Fréjus road tunnel excavated with conventional drill and blast methods and of its safety gallery excavated with a single shield tunneling boring machine. They exhibit a very interesting configuration of two tunnels excavated in parallel under the same geotechnical conditions but with different excavation techniques. Monitored geotechnical data from both tunnels are analyzed and compared. Numerical simulations of both tunnels have been carried out with Flac3D. An anisotropic creep model which includes weakness planes of given orientation embedded in a visco-elasto-plastic matrix has been used for describing the behavior of the ground. A back-analysis of convergence measurements of the Fréjus road tunnel has been carried out. The behavior of the ground identified from the Fréjus road tunnel is extrapolated to predict the response of the Fréjus safety gallery in terms of the stress state in the lining. The influence of the technique of excavation on the time-dependent parameters of the ground is taken into account in the computations and its effects are discussed. It is shown that the long term ground deformation are significantly reduced with TBM excavation as compared to traditional blast and drill method.Furthermore, the convergence-confinement methods are reviewed and their applicability is discussed when they are applied to full face circular tunnels excavated in rock masses with a stiff support system near the face. In this context, a set of empirical formula are proposed which allows to accurately predict the equilibrium state between the ground and the lining in circular tunnels excavated in full section. These formula are useful in the preliminary phase of tunnel design
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Persson, Daniel. "Visualisering av amyloider och patogenes i skadad näthinna." Thesis, Högskolan Kristianstad, Sektionen för lärande och miljö, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-17218.
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Ansamling av amyloid beta (Aβ) i de extracellulära miljöerna är associerad till många svåra sjukdomar som Alzheimers och ålders-relaterad makuladegeneration (AMD). Amyloider karaktäriseras av att de är olösliga, toxiska mot neuron och orsakar därför svår skada. AMD är den ledande orsaken till blindhet och irreversibelt förlorande av skarp syn då Aβ manifesterar i makula. I AMD orsakar Aβ inflammatorisk aktivitet där det retinala pigmentepitelet bryts ned och ljuskänsliga fotoreceptorer dör genom apoptos. Idag lever ca 150 miljoner människor med AMD där mänga har svårt att utföra vardagliga uppgifter till följd av förlust av skarp syn. Idag är Kongo röd en av de vanligaste metoderna för att visualisera amyloider in vitro. Den patogenes som orsakas av amyloider kan analyseras med immunofluorescens och immunohistokemi. Syftet med studien var att undersöka förekomst av amyloider i samband med celldöd i näthinna från gris, undersöka den patogenes som amyloider orsakar med immunofluorescens och immunohistokemi, samt undersöka om det finns korrelation mellan amyloider och celldöd. Resultatet visade att amyloider var förekommande i näthinnan och hade orsakat celldöd och ansamling av aggresomer. Amyloider och den patologi som orsakats kunde visualiseras i det yttre lagret av näthinnan.Deposition of amyloid beta (Aβ) in the extracellular environment are associated to some severe diseases, like Alzheimer’s disease and age-related macular degeneration (AMD). Amyloids are characterized by insolubility, toxicity towards neuron and are there-for damaging to tissues. AMD is the primary cause of blindness and irreversible loss of central vision through manifestation of Aβ in the macula. In AMD, Aβ drives an inflammatory action that degenerates the retinal pigment epithelium and cause atrophy of photoreceptors. Today ~150 million people live with AMD where many find difficulties performing everyday tasks due to loss of sharp vision. Congo red is a gold standard for visualizing amyloids in vitro and the pathogenesis caused by amyloids can be analyzed by immunofluorescence and immunohistochemistry. The purpose of this study was to show the presence of amyloids relating to cell death in pig retina, show the pathogenesis caused by amyloids by using immunofluorescence and immunohistochemistry, and investigate whether there is correlation between amyloids and cell death. The result showed that amyloids were present in the retina and caused cell death and gathering of aggresomes. Amyloids and the caused pathology could be visualized in the outer layer of the retina.
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Šebela, Kamil. "Chladící systém Formule Student." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2019. http://www.nusl.cz/ntk/nusl-399361.
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The thesis deals with Formula Student cooling system. The thesis is divided to three main parts, the first part deal about description of current cooling system and lubricating system. The second part of thesis deals with measuring individual component of system at measuring stand, wind tunnel and measuring at vehicle. The last part of thesis deal about evaluation of measurement and construction changes for improving parameters of the cooling system.
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Janyška, Miroslav. "Studie propojení mezi MÚK Ostrovačice na D1 a D52." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2019. http://www.nusl.cz/ntk/nusl-412948.
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This master’s thesis is a reconnaissance study developed to create a concept of road connection between the road 43 and the highway D52 in the Czech Republic. The possible route of road 43 is planned both in highway and road category in so called Bitysska trail and is connected to highway D1 at Ostrovacice interchange. This thesis suggests highway and road options to connect interchange Ostrovacice and highway D52 at the section between Ledce and Rajhrad. Suggested options were compared and the most convenient draft option was further worked out.
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Vaculovičová, Vanda. "Město místo továrny." Master's thesis, Vysoké učení technické v Brně. Fakulta architektury, 2011. http://www.nusl.cz/ntk/nusl-215829.
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Architectural and urban design for the reconversion of the part of the Old Brno (former textil fabric of the company Kras and surrounding areas) based on the existing structure of the Old Brno and ideas of compact city (mixing functions> multipurpose buildings, the divergence between private and public space, higher density of the city ...) The work consists of several parts: planning studies, design transportation solutions, waterfront Svratka concept and design for the multifunctional block on the waterfront. What is important is the idea of calming waterfront Svratka (sheltering services to the proposed tunnel and the cancellation of barrier between the city and the river). The waterfront district should become after more attractive location and provide a space for sport, recreation and afternoon walks. There is a block of multipurpose buildings on the waterfront. A corporate underground garage, which is under the block, should be build by a strong investor / city. After underground garage completion, the site will be divided into several parcels and these will be rented to various long-term investors .Tenants can build buildings on them according to their needs. It is possible to build buildings above the garage in sequence development. The project is just one of many options. Individual buildings are design in scale 1:200
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Hopkins, (nee Kaup) Marianne. "Characterization of Genes involved In Development and Senescence." Thesis, University of Waterloo, 2006. http://hdl.handle.net/10012/1257.
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Plant development is complex and highly regulated. Tens of thousands of genes have been sequenced for the model plant Arabidopsis thaliana, yet few have been functionally annotated and characterized. This thesis describes the expression analysis and characterization of four genes in Arabidopsis. Three of these belong to the eukaryotic translation initiation factor 5A (eIF5A) gene family, and the fourth encodes diacylglycerol acyltransferase 1 (DGAT1). Putative roles for these genes in the development of Arabidopsis thaliana are described. eIF5A is the only known protein to contain the amino acid hypusine. It has been demonstrated previously that eIF5A acts as a shuttle protein, moving specific mRNAs from the nucleus to the cytoplasm for translation. In Arabidopsis thaliana (At), there are three isoforms of eIF5A, and it is clear from the present study that they each have a unique temporal and spatial expression pattern. AteIF5A-1 and -2 are up-regulated during natural senescence and wounding/pathogenesis, respectively, and it is proposed that they regulate the onset of programmed cell death during these events. AteIF5A-3 is up-regulated in elongating meristem of the root, and it is proposed that this isoform is involved in cell growth.
Over-expression of the individual AteIF5A isoforms in planta resulted in pleiotropic phenotypes. When AteIF5A-1 or AteIF5A-2 was over-expressed, the phenotypes observed were indicative of their putative roles in the translation of proteins required for programmed cell death. When AteIF5A-3 was over-expressed, the phenotypes were indicative of a role for this protein in the regulation of cell and tissue elongation.
Lipid analysis of rosette leaves from Arabidopsis thaliana revealed an accumulation of triacylglycerol with advancing leaf senescence coincident with an increase in the abundance and size of plastoglobuli. The terminal step in the biosynthesis of triacylglycerol in Arabidopsis is catalyzed by DGAT1. When gel blots of RNA isolated from rosette leaves at various stages of development were probed with the Arabidopsis EST clone, E6B2T7, which has been annotated as DGAT1, a steep increase in DGAT1 transcript levels was evident in the senescing leaves coincident with the accumulation of triacylglycerol. The increase in DGAT1 transcript correlated temporally with enhanced levels of DGAT1 protein detected immunologically. Two lines of evidence indicated that the triacylglycerol of senescing leaves is synthesized in chloroplasts and sequesters fatty acids released from the catabolism of thylakoid galactolipids. First, triacylglycerol isolated from senescing leaves proved to be enriched in hexadecatrienoic acid (16:3) and linolenic acid (18:3), which are normally present in thylakoid galactolipids. Second, DGAT1 protein in senescing leaves was found to be associated with chloroplast membranes. These findings collectively indicate that DGAT1 plays a role in senescence by sequestering fatty acids de-esterified from galactolipids into triacylglycerol.
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Patrick, Sean Mark. "Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays." Diss., University of Pretoria, 2008. http://hdl.handle.net/2263/26120.
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Endocrine disrupting chemicals (EDCs) such as DDT have the ability to disrupt hormonally controlled processes, such as spermatogenesis, which is the maturation of germ cells into spermatozoa. During normal spermatogenesis, germ cell apoptosis can occur, but the degree of apoptosis within the testis could possibly be affected by exposure to EDCs. In 2004, a pilot study on the reproductive health of two freshwater fish species, Oreochromis mossambicus and Clarias gariepinus, from three impoundments in the Luvuvhu River, found concerning levels of DDT and its metabolites in both species from the Nandoni Dam, and in O. mossambicus from the Xikundu Weir. This was not surprising as a large part of the Luvuvhu River catchment is located within an area where ongoing DDT-spraying occurs for vector control purposes. Hence, in 2006, a larger WRC-funded project began to further investigate the findings from the pilot study. A subsidiary study, spanning two seasons, was initiated to investigate testicular apoptosis in fish from the polluted systems, the Nandoni Dam (ND) and the Xikundu Weir (XW), as well as a reference site, the Albasini Dam (AD), utilizing caspase-3 and TUNEL immunoexpression as apoptotic markers. In addition, three fixatives, Bouin’s Fluid (BF), Neutrally Buffered Formalin (NBF) and Paraformaldehyde (PFA), were used to determine which would be the optimal fixative for both histological and immunohistochemical assessments. Sampling occurred during season 1, the low-flow season (October 2007), during DDT spraying of the surrounding area, and season 2, the high–flow season (February 2008), two months after the DDT-spraying was completed. The testes of O. mossambicus (n = 19 season 1, n = 25 season 2) and C. gariepinus (n = 19 season 1, n = 20 season 2) were fixed in the above-mentioned fixatives, embedded in paraffin wax, prepared for immunohistochemistry, and exposed to caspase-3 antibodies and TUNEL antibodies individually. The results indicated that the residues of p,p´-DDT - DDD and - DDE were found in the fat samples of both O. mossambicus and C. gariepinus, in AD, ND and XW. Testicular apoptotic assessment using the caspase-3 assay clearly labeled spermatocytes in the process of cellular death in both seasons, in all three fixatives. When comparing the two assays, a significant difference is found between the caspase-3 and TUNEL positive cells. The results further show that, when comparing the three sampling sites, the highest amount of positive cells are found at the XW. The decrease observed in season two, in both the caspase-3 and TUNEL assay may possibly be linked to the stage of spermatogenesis, coinciding with hormonal changes associated with the different sampling seasons (i.e. breeding and non-breeding seasons). The levels of DDT found in the fat tissue, could not be correlated to an up-regulation in apoptotic cells. The results The results indicated that the choice of fixative, could affect the identification of the amount of positive cells. The utility of the caspase-3 and TUNEL assays, in conjunction with all three fixatives, proves a successful tool in assessing and quantifying modulated testicular apoptosis, creating greater research potential in the assessment of the effects of aquatic pollution. CopyrightDissertation (MSc)--University of Pretoria, 2008.
Physiology
unrestricted
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Hoang, Thi My Linh. "Engineering salinity tolerance in rice by exogenous expression of cell death regulators." Thesis, Queensland University of Technology, 2014. https://eprints.qut.edu.au/72793/1/Thi%20My%20Linh_Hoang_Thesis.pdf.
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Rice, an important crop that feeds more than half of the world's population is very sensitive to salinity stress – a growing problem affecting crop production globally. This PhD study addressed this problem by manipulating the programmed cell death pathways in rice resulting in significant enhancement of salinity stress tolerance. The impact of this work is that farmers would be in a position to grow rice containing such a trait in environments where salinisation of the soil exists, thereby addressing food security needs.
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Miedlich, Anja [Verfasser], Syed G. [Akademischer Betreuer] Haider, and Anja [Akademischer Betreuer] Lorch. "Eine methodenkritische Studie zum histochemischen Nachweis der Apoptose mittels TUNEL im Hodengewebe der Wistarratte / Anja Miedlich. Gutachter: Syed G. Haider ; Anja Lorch." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2015. http://d-nb.info/1067553320/34.
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