Journal articles: 'TULSI(OCIMUM TENUIFLORUM)'

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Manaswini, N. K., S. Nazneen, Garige Baba Shankar Rao, Boggula Narender, Bakshi Vasudha, and Manda Ram Mohan. "Evaluation of Ocimum tenuiflorum and Syzygium aromaticum phenolic ethereal oils for In-vitro anti-inflammatory and anti-bacterial activities." Journal of Drug Delivery and Therapeutics 9, no. 2 (March 15, 2019): 93–96. http://dx.doi.org/10.22270/jddt.v9i2.2383.

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Aim: The present study is aimed to evaluate in-vitro anti-inflammatory and anti-bacterial activity of phenolic ethereal oils like Tulsi (Ocimum tenuiflorum) and Clove (Syzygium aromaticum). Materials and methods: A total of 500 g of fresh leaves and dried flower buds of Tulsi and Clove were subjected to hydro-distillation method for 6 h using Clevenger’s apparatus. The isolated ethereal oils were used for testing the in-vitro anti-inflammatory activity by using albumin denaturation assay, proteinase inhibitory activity at a concentration of 20, 40, 60, 80 and 100 µl/ml and anti-bacterial activity against two gram positive microorganisms (Bacillus subtilis and Staphylococcus aureus) and two gram negative microorganisms (Salmonella typhi and Escherichia coli) at concentrations 50 µl/ml, 100 µl/ml and 200 µl/ml by adopting cup plate method. Results: The isolated ethereal oils exhibited significant in-vitro anti-inflammatory effect and also inhibited the growth of both Gram positive and Gram negative microorganisms at 50 µl/ml, 100 µl/ml and 200 µl/ml concentrations. Conclusion: The findings of this study showed that the effectiveness of Phenolic ethereal oils isolated from Clove and Tulsi. Clove (Syzygium aromaticum) showed enhanced anti-inflammatory and anti-bacterial activity compared to Tulsi (Ocimum tenuiflorum). The present study provides evidence that Ocimum tenuiflorum and Syzygium aromaticum; Phenolic ethereal oils contain medicinally important bioactive components justifying its traditional use. Keywords: Phenolic ethereal oils, anti-inflammatory activity, anti-bacterial activity, Ociumm tenuiflorum, Syzygium aromaticum.

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Santwani, Dr Soniya. "Medical use of Tulsi Plant and its Importance for Human Beings." International Journal for Research in Applied Science and Engineering Technology 11, no. 3 (March 31, 2023): 859–61. http://dx.doi.org/10.22214/ijraset.2023.49494.

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Abstract: Ocimum tenuiflorum (synonym Ocimum sanctum), commonly known as holy basil, tulsi is an aromatic perennial plant. Tulsi is a plant that has many medicinal properties. It has an excellent analeptic effect that can heal the mind as well as the body. Tulsi as properties antioxidant and anti-inflammatory reduces inflammation by maintaining the nerves and the tissues that are damaged by harmful free radicals.

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Bhamra, Sukvinder Kaur, Michael Heinrich, Mark R. D. Johnson, Caroline Howard, and Adrian Slater. "The Cultural and Commercial Value of Tulsi (Ocimum tenuiflorum L.): Multidisciplinary Approaches Focusing on Species Authentication." Plants 11, no. 22 (November 18, 2022): 3160. http://dx.doi.org/10.3390/plants11223160.

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Tulsi (Holy basil, Ocimum tenuiflorum L., Lamiaceae), native to Asia, has become globalised as the cultural, cosmetic, and medicinal uses of the herb have been popularised. DNA barcoding, a molecular technique used to identify species based on short regions of DNA, can discriminate between different species and identify contaminants and adulterants. This study aimed to explore the values associated with Tulsi in the United Kingdom (UK) and authenticate samples using DNA barcoding. A mixed methods approach was used, incorporating social research (i.e., structured interviews) and DNA barcoding of Ocimum samples using the ITS and trnH-psbA barcode regions. Interviews revealed the cultural significance of Tulsi: including origins, knowledge exchange, religious connotations, and medicinal uses. With migration, sharing of plants and seeds has been seen as Tulsi plants are widely grown in South Asian (SA) households across the UK. Vouchered Ocimum specimens (n = 33) were obtained to create reference DNA barcodes which were not available in databases. A potential species substitution of O. gratissimum instead of O. tenuiflorum amongst SA participants was uncovered. Commercial samples (n = 47) were difficult to authenticate, potentially due to DNA degradation during manufacturing processes. This study highlights the cultural significance of Tulsi, despite a potential species substitution, the plant holds a prestigious place amongst SA families in the UK. DNA barcoding was a reliable way to authenticate Ocimum species.

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Chowdhury, Tanmay, Goutam Kumar Basak, Putchen Dakshinamoorthy Deepalakshmi, Soumen Saha, and Amitava Mandal. "Metabolomics using Gas chromatography-mass spectrometry and antibacterial activity of nine Ocimum taxa of Dakshin Dinajpur district, West Bengal, India." Journal of Applied and Natural Science 13, no. 1 (February 25, 2021): 127–36. http://dx.doi.org/10.31018/jans.v13i1.2508.

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Ocimum traditionally known as Holy Basil or Tulsi is an available herb plenty across the country. Traditionally, it is used against a number of human diseases. In this present study, the metabolites present in the ethanolic extracts of nine Ocimum taxa, O. tenuiflorum L. (Green and purple type) two morphotypes of O. basilicum L., (Babu and Marua tulsi) two morphotypes of O. gratissimum L. (Ram and Ajowan tulsi) and each one from O. americanum L. (Bon tulsi), O. × africanum Lour. (Lebu tulsi), and O. kilimandscharicum Guerke.grown naturally in Dakshin Dinajpur district, West Bengal, India were identified using Gas chromatography–mass spectrometry (GC-MS). Among the identified metabolites, carbohydrates, aliphatic alcohols, aliphatic acids, fused ring aromatic hydrocarbon, amino acids, phenolic compounds, quinone, steroids, terpenoids and vitamin E were the chief constituents. The occurrence of these metabolites describes the high biological activity of Ocimum species. Furthermore, in vitro antibacterial activities were also identified against four bacterial strains, Staphylococcus aureus (MTCC 96), Bacillus cereus (MTCC 1305), Proteus vulgaris (MTCC 1771) and Escherichia coli (MTCC 2939). Tested bacterial strains were taken from the Institute of Microbial Technology, Chandigarh, India The results indicated that the ethanolic extracts of all the nine Ocimum taxa have satisfactory minimum inhibitory concentration (MIC) values against the tested microorganisms. O. tenuiflorum has the highest (91.03 %) metabolic content whereas O. × africanum has the minimum. This would definitely serve as a scientific basis of the traditional use of basil against human ailments.

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Rajagopal, Ponnulakshmi. "Molecular docking analysis of PARγ with compounds from Ocimum tenuiflorum." Bioinformation 17, no. 11 (November 30, 2021): 928–31. http://dx.doi.org/10.6026/97320630017928.

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The ligand-activated transcription factor peroxisome proliferator-activated receptor (PPAR) has become a major target for type 2 diabetes. It belongs to the nuclear receptor superfamily, which controls the expression of proteins involved in glucose metabolism, lipid metabolism, adipocyte proliferation and differentiation, and insulin sensitivity. Ocimum tenuiflorum, often known as Krishna tulsi, is the most sacred herb in India. It was utilized for a variety of medicinal purposes. Therefore, it is of interest to document the molecular docking analysis data of PARγ modulators from Ocimum tenuiflorum. Four of the twenty substances (rosmarinic acid, permethrin, luteolin, and isosakuranetin) have a considerable binding affinity for the PPARγ. These phytochemicals are a source of potential anti-diabetic medicines.

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Parajuli-Baral, Kalpana. "Formulation and Evaluation of Quality Parameters of Effervescent Granules from the Potent Antioxidant between Two Variants of the Adaptogenic Herb Ocimum tenuiflorum L." Scientific World Journal 2023 (April 25, 2023): 1–8. http://dx.doi.org/10.1155/2023/2050846.

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Ocimum tenuiflorum L. is found throughout semitropical and tropical parts of Southeast Asia. In Nepal, O. tenuiflorum L. is popular with two variants: Krishna Tulsi consisting of purple-colored leaves and Sri Tulsi consisting of green-colored leaves. O. tenuiflorum L. is considered the queen of herbs and is a traditionally and clinically proven medicinal herb for its application and efficacy. However, no commercial pharmaceutical preparations of O. tenuiflorum L. are available using effervescent vehicles. Therefore, the present study aimed to compare the antioxidant activity of leaves from the two varieties of O. tenuiflorum L. and formulate and evaluate the quality parameters of effervescent granules of the potent extract. The antioxidant activity of O. tenuiflorum L. ethanolic extracts was evaluated by DPPH radical scavenging assay at 1, 10, and 100 µg/mL concentrations, where ascorbic acid was used as the positive control. The antioxidant activity of purple-leafed O. tenuiflorum L. was found to be higher than that of green-leafed O. tenuiflorum L. Therefore, effervescent granules of the ethanolic extract of purple-leafed O. tenuiflorum L. were formulated using the pharmaceutical excipients tartaric acid, citric acid, and sodium bicarbonate and the quality parameters of the granules were evaluated. The formulated granules met the quality parameters assessed from the angle of repose, bulk density, tapped density, Carr’s Index, Hausner’s ratio, effervescent cessation time, and stability studies. Thus, the formulated effervescent granules of O. tenuiflorum L. can be used for therapeutic purposes or as a functional food.

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Harikumar, P. S., and C. M. Manjusha. "Study on the antibacterial activity of selected natural herbs and their application in water treatment." Drinking Water Engineering and Science Discussions 6, no. 2 (August 22, 2013): 199–231. http://dx.doi.org/10.5194/dwesd-6-199-2013.

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Abstract. The microbial contamination of water is a world-wide environmental problem. Many traditional methods are being used in various parts of the world to purify the water. According to World Health Organization, 80% of world's population living in rural areas relies on herbal traditional medicines as their primary health care. So the study on properties and uses of medicinal plants are subject to growing interests. An attempt was made to assess the antibacterial properties of certain selected herbs such as Ocimum sanctum (Krishna Tulsi), Ocimum kilimandscharicum (Karpoora Tulsi), Ocimum tenuiflorum (Ram Tulsi), Azadirachta indica (Neem), Simarouba glauca (bitterwood), Caesalpinia sappan (Pathimugam), Cuminum cyminum (Jeerakam), Vetiveria zizanioides (Ramacham), Saraca indica (Ashoka tree) and Murraya koenigii (curry leaves) against different bacteria such as total coliforms, faecal coliforms, Escherichia coli, Bacillus sp. and Serratia sp. The antibacterial activity of the plant extracts was determined by spread plate method, Kirby–Bauer disc diffusion method, most probable number (MPN) method and Petrifilm method. The shelf life of the herbal extract Ocimum sanctum (Krishna Tulsi) was also determined using a UV-visible spectrophotometer. A comparison study of the antibacterial efficiency of the three varieties of Ocimum sanctum, Ocimum kilimandscharicum and Ocimum tenuiflorum was also done. After the complete analysis of the antibacterial activity of different herbs, Ocimum sanctum, the most efficient herb, was selected and treatment methods based on the herb were developed so that it can be used conveniently in various households. Therefore Ocimum sanctum plant can be further subjected to isolation of therapeutic antimicrobial and pharmacological evaluation.

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Parveen, Tahira, and Kanika Sharma. "BIOASSAY OF BOTANICAL OILS AGAINST ALTERNARIA SOLANI." International Journal of Current Pharmaceutical Research 10, no. 4 (July 16, 2018): 19. http://dx.doi.org/10.22159/ijcpr.2018v10i4.27660.

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Objective: In vitro antifungal activity of six essential oils of 1. Ocimum tenuiflorum (Purple leaves/Krishna Tulsi) 2. Cymbopogon citrates (Nimbu ghas), 3. Origanum majorina (Sweet majoram), 4. Ocimum citriodorum (Nimbu tulsi), 5. Ocimum bascillicum (Gulal tulsi), and 6. Ocimum sanctum (Green leaves/Shree tulsi) were investigated against economically important phytopathogenic fungi, Alternaria solani, isolated from infected chilly. Methods: The experiment was carried out by Whatman paper disc method using Whatman paper No.3 on Potato Dextrose Agar with three replicates. Five concentrations of each essential oils i.e., 20, 40, 60, 80 and 100% were assayed against the test fungus. The experiment was carried out at 27ᵒC and mycelial growth was measured after every third day, upto 15 d using statistical method.Results: It is an evident from this study that all the oils used in this study are inhibiting test fungi, oil of O. bascillicum and O. sanctum are completely (100%) inhibiting test fungi.Conclusion: It may be concluded from the above study that all the taken oils have a good potential to control plant pathogenic fungi and these could be considered for developing a new fungicide.

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Khurana, Prakash. "A REVIEW ON MEDICINAL USES OF OCIMUM TENUIFLORUM LINN (TULSI)." Journal of Medical Pharmaceutical And Allied Sciences 9, no. 3 (June 15, 2020): 2509–17. http://dx.doi.org/10.22270/jmpas.v9i3.950.

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Ervina, Ervina, and Jonathan Marvell. "Investigating sensory characteristics and consumer preferences of functional beverages made from Tulsi (Ocimum tenuiflorum Linne)." IOP Conference Series: Earth and Environmental Science 1200, no. 1 (June 1, 2023): 012006. http://dx.doi.org/10.1088/1755-1315/1200/1/012006.

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Abstract Tulsi is widely known as a medicinal plant and possesses many health benefits. The pharmacological study regarding the compounds in tulsi has been widely studied, but the sensory characteristic and consumer acceptance of this plant has never been conducted previously. This study aims to investigate the sensory characteristics of functional beverages made from tulsi leaves and to determine which sensory attributes that able to promote consumers’ acceptance. Three samples of tulsi beverages were evaluated by the trained panels (n=14) using Quantitative Descriptive Analysis (QDA) while the acceptance test was assessed by 61 consumers. A total of 27 key attributes of tulsi beverages were generated during the QDA evaluation. All the samples have different sensory characteristics. The aroma and taste of ginger, citrus, lemongrass, basil, mint, honey, sweetness, and aftertaste of the hot sensation of ginger were positively associated with consumers’ liking. In contrast, bitter aroma, watery-like texture, astringent and astringent aftertaste were contribute to consumers’ disliking. This study could be used as a preliminary study for developing new functional beverage products from tulsi leaves by considering the sensory characteristic and key attributes for the liking of tulsi beverages.

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Marvell, J., and E. Ervina. "Sensory profiling of beverages from Tulsi leaves (Ocimum tenuiflorum Linne) using Quantitative Descriptive Analysis (QDA)." IOP Conference Series: Earth and Environmental Science 1115, no. 1 (December 1, 2022): 012098. http://dx.doi.org/10.1088/1755-1315/1115/1/012098.

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Abstract Tulsi, also known as holly basil, are well known for their medicinal, pharmacological, and health benefits. However, the sensory profiles of food or beverages made from this plant have never been reported before. This study aimed to define the sensory characteristics of beverages made from tulsi leaves using the Quantitative Descriptive Analysis (QDA) method. Fourteen trained panelists were selected and trained, three samples of tulsi beverages were evaluated and the tests were replicated twice. In total, 27 sensory attributes were generated. The dominant attributes include yellow, brown and transparent colors for the visual aspect, representing tea-like beverage. Moreover, both the odor and flavor of basil, mint and grassy were also perceived as key attributes for the tulsi beverages. In addition, the tulsi beverages were also characterized by strong astringent taste and aftertaste. In general, the test was reliable, however, additional training is needed to train the panelists intensively for the selected attributes. The results obtained from this research can be used as a preliminary study to develop new functional food products made from tulsi leaves by considering their sensory profiles.

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Sutar, S. R., S. D. Surbhaiyya, D. R. Dhumale, V. B. Kasar, A. P. Bhole, and V. R. Kulkarni. "Detection and Comparison of RAPD & SSR Primers in Genetic Diversity of Ocimum sanctum." International Journal of Environment and Climate Change 13, no. 8 (June 13, 2023): 1529–38. http://dx.doi.org/10.9734/ijecc/2023/v13i82102.

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Aims: To Analysis of genetic variability in Tulsi (Ocimum sanctum) genotypes by using RAPD & SSR Markers. Place and Duration of Study: Department of Plant Biotechnology at K.K.Wagh College of Agricultural Biotechnology, Nashik. Methodology: Ocimum tenuiflorum Linn., commonly known as Tulsi, is an aromatic plant with significant traditional and medicinal value. To assess the genetic diversity and relatedness of six Tulsi genotypes (Krishna, Ram, Lavangi, Pandharpuri, Daisil, and Kapoori), molecular techniques were employed. The genotypes were collected from Nagarjuna Medicinal and Aromatic Plant Park at Dr. P.D.K.V. Akola. DNA isolation was performed using alcohol fixation without liquid nitrogen, and the genotypes were analyzed using RAPD and SSR primers for molecular characterization. Results: Genetic diversity analysis of six Tulsi genotypes (Krishna, Ram, Lavangi, Pandharpuri, Daisil, and Kapoori) was performed using RAPD and SSR markers. Five RAPD primers produced 15 bands, with 11 bands showing polymorphism (73.3%) and 4 bands showing monomorphism (26.7%). The PIC value ranged from 0.28 to 0.49 (average: 0.40). Four SSR primers generated 9 bands, with 8 bands showing polymorphism and 1 band showing monomorphism. The PIC value ranged from 0.24 to 0.57 (average: 0.39). The Jaccard coefficient revealed moderate to high similarity in RAPD (0.40 to 0.73) and SSR (0.44 to 0.88) analyses. The UPGMA dendrogram separated the genotypes into two main clusters. Cluster 1 included Krishna, Lavangi, Ram, Pandharpuri, and Daisil Tulsi, while cluster 2 consisted of Kapoori Tulsi. The SSR dendrogram also formed two clusters, with Krishna, Lavangi, Ram, Daisil, and Kapoori genotypes in cluster 1, and Pandharpuri Tulsi showing dissimilarity and forming cluster 2.

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Travadi, Tasnim, Sonal Sharma, Ramesh Pandit, Mital Nakrani, Chaitanya Joshi, and Madhvi Joshi. "A duplex PCR assay for authentication of Ocimum basilicum L. and Ocimum tenuiflorum L in Tulsi churna." Food Control 137 (July 2022): 108790. http://dx.doi.org/10.1016/j.foodcont.2021.108790.

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Jürges, Gabriele, Kathrin Beyerle, Michael Tossenberger, Annette Häser, and Peter Nick. "Development and validation of microscopical diagnostics for ‘Tulsi’ (Ocimum tenuiflorum L.) in ayurvedic preparations." European Food Research and Technology 229, no. 1 (February 26, 2009): 99–106. http://dx.doi.org/10.1007/s00217-009-1030-3.

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Kaur, Pinderpal, Sanju Bala Dhull, Kawaljit Singh Sandhu, Raj Kumar Salar, and Sukhvinder Singh Purewal. "Tulsi (Ocimum tenuiflorum) seeds: in vitro DNA damage protection, bioactive compounds and antioxidant potential." Journal of Food Measurement and Characterization 12, no. 3 (March 7, 2018): 1530–38. http://dx.doi.org/10.1007/s11694-018-9768-6.

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Bast, Felix, Pooja Rani, and Devendra Meena. "Chloroplast DNA Phylogeography of Holy Basil (Ocimum tenuiflorum) in Indian Subcontinent." Scientific World Journal 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/847482.

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Ocimum tenuiflorumL., holy basil “Tulsi”, is an important medicinal plant that is being grown and traditionally revered throughout Indian Subcontinent for thousands of years; however, DNA sequence-based genetic diversity of this aromatic herb is not yet known. In this report, we present our studies on the phylogeography of this species usingtrnL-trnFintergenic spacer of plastid genome as the DNA barcode for isolates from Indian subcontinent. Our pairwise distance analyses indicated that genetic heterogeneity of isolates remained quite low, with overall mean nucleotidep-distance of5×10-4. However, our sensitive phylogenetic analysis using maximum likelihood framework was able to reveal subtle intraspecific molecular evolution of this species within the subcontinent. All isolates except that from North-Central India formed a distinct phylogenetic clade, notwithstanding low bootstrap support and collapse of the clade in Bayesian Inference. North-Central isolates occupied more basal position compared to other isolates, which is suggestive of its evolutionarily primitive status. Indian isolates formed a monophyletic and well-supported clade withinO. tenuiflorumclade, which indicates a distinct haplotype. Given the vast geographical area of more than 3 million km2encompassing many exclusive biogeographical and ecological zones, relatively low rate of evolution of this herb at this locus in India is particularly interesting.

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Pawar, Pradnya H., and Sharmila R. Chaudhari. "Size controlled Bio-directed synthesis of Ag Metal Nanoparticles using Tulsi (Ocimum tenuiflorum) leaves extract." Asian Journal of Research in Chemistry 10, no. 5 (2017): 646. http://dx.doi.org/10.5958/0974-4150.2017.00109.2.

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Harini, Prakash, Raju Balaji, and Madasamy Parani. "The complete chloroplast genome of Ocimum tenuiflorum L. subtype Rama Tulsi and its phylogenetic analysis." Mitochondrial DNA Part B 6, no. 8 (July 5, 2021): 2224–26. http://dx.doi.org/10.1080/23802359.2021.1944381.

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Kavya, NM, Raju Balaji, Tanuja, Madasamy Parani, and Palanisamy Senthilkumar. "The complete chloroplast genome of Ocimum tenuiflorum L. subtype Krishna Tulsi and its phylogenetic analysis." Mitochondrial DNA Part B 6, no. 8 (July 15, 2021): 2358–60. http://dx.doi.org/10.1080/23802359.2021.1951133.

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Taneja, Tanvi, Indu Sharma, Himani Garg, and Raj Singh. "SCREENING OF ENDOPHYTE BACTERIA FOR BIOPROSPECTING POTENTIAL FROM SYZYGIUM CUMINI (JAMUN) AND OCIMUM TENUIFLORUM (TULSI)." PLANT ARCHIVES 23, Suppliment-1 (April 15, 2023): 274–79. http://dx.doi.org/10.51470/plantarchives.2023.v23.no1.046.

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Pratap Toppo, Pankaj, Mohit Anand Sandiman Himanshu, Dayanand Sai Painkra Khumita Verma, and Anish mankur Shani Raj. "Effect of Organic Manure on Plant Growth Attributes of Krishna Tulsi [Ocimum tenuiflorum (L.)] Under Eucalyptus (Eucalyptus tereticornis) Based Agroforestry System." International Journal of Current Microbiology and Applied Sciences 11, no. 10 (October 10, 2022): 149–57. http://dx.doi.org/10.20546/ijcmas.2022.1110.018.

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A field experiment was conducted at the Herbal Garden of Department of Forestry, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.). During the season of 2021-22 with a view to study the “Effect of organic manure on plant growth attributes of Krishna Tulsi [Ocimum tenuiflorum (L.)] Under Eucalyptus (Eucalyptus tereticornis) based Agroforestry system”. The field experiment was conducted on Krishna Tulsi intercrops under Eucalyptus tree crop were used to grown and treatment was replicated three times in Randomized Block Design (RBD) with plant spacing 40cm × 40cm. The soil of experimental field was clay to loam soil. The investigation, There were The fertilizer mixture FYM and vermicompost were used, which were applied at different concentrations in Tulsi intercrop in eight treatments viz., T1: - FYM 100%, T2: - Vermicompost 100%, T3:- Neem cake 100 %, T4:- FYM 75% + Vermicompost 25 %, T5:- FYM 75% + Neem Cake 25%, T6:- Vermicompost 50% + Neem Cake 50%, T7: - FYM 50 % + Neem Cake 25 %+ Vermicompost 25 % and T8:- Control (No Manures). The crop growth parameters i.e., Plant height (cm), Number of branches plant-1 and Collar diameter (mm) were significantly superior in the treatment T2 (Vermicompost 100%) at different crop growth stages at 30, 60, 90 and at time of harvest. On the basis of above findings, treatment T2 (Vermicompost 100%) stand could be better performance first in position and T1 (FYM 100%) stand in second order of preference. However, treatment T4 (FYM 75% + Vermicompost 25 %) comes in next in order. Therefore, it may be concluded that treatment T2 (Vermicompost 100%) may be prefer for higher growth in crop Krishna Tulsi.

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Beatovic, Damir, Slavica Jelacic, Cedo Oparnica, Dijana Krstic-Milosevic, Jasmina Glamoclija, Mihailo Ristic, and Jovana Siljegovic. "Chemical composition, antioxidative and antimicrobial activity of essential oil Ocimum sanctum L." Chemical Industry 67, no. 3 (2013): 427–35. http://dx.doi.org/10.2298/hemind120615086b.

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Ocimum sanctum L. (Lamiaceae) sin. Ocimum tenuiflorum L. or Tulsi basil is a plant originating from tropical and subtropical areas of India. It is used in both the traditional and official medicine in India. Tulsi is a type of basil that is insufficiently explored and studied in Europe. The goal of this paper is to determine the chemical composition, antioxidative, and antimicrobial activity of the essential oil Ocimum sanctum L. grown in Serbia. The quantity of essential oil in 100 g of herb (v/w) is 0.68%, with 41 components identified in the tested essential oil. The most represented chemical group are sesquiturpene hydrocarbonates with 80.47%. Other groups were much less represented. Sesquiturpene hydrocarbonate ?-cariophyllene is a predominant component in the essential oil with 63.80%. The quantity of tested essential oil needed to achieve 50% of inhibition of DPPH radicals is 0.35 ?g/ml, and it has high potential to neutralize free radicals. The essential oil exhibited antibacterial activity to all tested strains of bacteria, both Gram-positive and Gram-negative. It affected all strains in an inhibitory way in the interval 0.34-41.50 ?l/ml, and in a bactericide way within the range 22.50-124.5 ?l/ml. The most sensitive strains of bacteria are Salmonella typhimurium and Escherichia coli, while Listeria monocytogenes and Enterococus faecalis showed greatest resistance. The essential oil exhibited antifugal activity on all tested fungi. It affected all tested fungi in an inhibitory way in the interval 4.42-8.83 ?l/ml, and in a microbicide way within the range 10.00-50.00 ?l/ml. The most sensitive fungi are: Aspergillus ochraceus, Penicillium ochrochloron and Penicilium funiculosum, while the most resistent one is Aspergillus niger. The tested basil essential oil Ocimum sanctum demonstrated significant antioxidative and antimicrobial effect and may be used as a raw material in food, pharmaceutical and chemical industries.

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Bhat, Vishnu, Fathima Shayima, Fathima Suhera KA, and Naseeha Farzana. "FORMULATION AND EVALUATION OF HERBAL CREAM." Journal of Biological & Scientific Opinion 11, no. 1 (January 26, 2023): 1–4. http://dx.doi.org/10.7897/2321-6328.111171.

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The aim of the present study was to formulate and evaluate the herbal cream having properties of moistening, nourishing, anti-acne and antiinflammatory effect on the skin using different crude drugs like Azadirachta indica (Neem), Aloe barbadensis (Aloe Vera), Ocimum tenuiflorum (Tulsi). The cream was prepared by using the cream base i.e. bee’s wax, liquid paraffin, borax, methyl paraben, distilled water, rose oil, Aloe Vera gel, aqueous extract of neem and tulsi. The cream was prepared by homogeneous mixing of all the excipients and the herbal extract. By using this method, we have prepared six batches F1, F2, F3, F4, F5, and F6. Formulations were evaluated for different parameters like physical evaluation, irritancy study observation, wash ability, spread ability, PH, Viscosity, phase separation, greasiness observations, dye test, homogeneity, acid value, saponification value. All the six formulations F1, F2, F3, F4, F5, and F6 showed good appearance, PH, adequate viscosity and no phase separation was observed. All the six formulations F1, F2, F3, F4, F5, and F6 were stable and can be safely use on the skin.

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Joshi, R. K., and S. L. Hoti. "Chemical composition of the essential oil of Ocimum tenuiflorum L. (Krishna Tulsi) from North West Karnataka, India." Plant Science Today 1, no. 3 (July 1, 2014): 99–102. http://dx.doi.org/10.14719/pst.2014.1.3.52.

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Sachyuka, Tatsiana, Alena Feskova, Natallia Kovalenko, Galina Supichenko, and Viktar Bosak. "Spices, Herbs, and Essential-Oil Plants: Yield and Fatty Acid Composition of Seeds." Food Processing: Techniques and Technology 52, no. 4 (December 21, 2022): 675–84. http://dx.doi.org/10.21603/2074-9414-2022-4-2397.

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Spices, herbs, and essential-oil plants provide a lot of marketable products, e.g., green mash, seeds, essential oils, etc. These raw materials find application in food industry, pharmacy, perfumery, traditional and folk medicine, landscape gardening, etc. The research objective was to test some new varieties of spices, aromatic herbs, and essential oil crops for their yield and fatty acid composition. The study featured new varieties of medicinal hyssop (Hyssopus officinalis L.), oregano (Origanum vulgare L.), basil (Ocimum basilicum L.), tulsi (Ocimum tenuiflorum L.), garlic chives (Allium odorum L.), rue (Ruta graveolens L.), blue fenugreek (Trigonella caerulea (L.) Ser.), and big-root geranium (Geranium macrorrhizum L.). The yield of green mass and seeds was studied on the experimental field of the Belarusian State Agricultural Academy according to standard methods. A set of standard laboratory procedures made it possible to define the content of crude fat, while the fatty acid composition of seed lipids was studied by gas chromatography in extracts of methyl esters of fatty acids. The green mass yield was 150–280 c/ha, whereas the seed yield was 0.5–4.0 c/ha; the crude fat content was 1.15–3.37 and 1.62–9.81%, respectively. The fatty acid composition of seed lipids included caprylic, palmitic, stearic, oleic, linoleic, and α-linolenic acids. The highest content of polyunsaturated essential fatty acids represented by linoleic and α-linolenic acids was observed in oregano (86.74–87.27%), hyssop (76.41–85.96%), tulsi (85.67%), basil (72.52–80.72%), rue (78.04%), and blue fenugreek (72.96%). The specified yield and fatty acid composition provided a complete assessment of spices, herbs, and essential-oil plants with the prospect of their use as part of new functional products.

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Paul, Souvik, Tanmoy Dey, Prabin Koirala, Sidhant Tamang, Soumik Bhattacharya, and Rajat Das. "Formulation and evaluation of Polyherbal tablet by using Neem, Tulsi, Turmeric and Ginger extract." Journal of Drug Delivery and Therapeutics 13, no. 7 (July 15, 2023): 46–51. http://dx.doi.org/10.22270/jddt.v13i7.5895.

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Introduction: Cold and cough is the common disease condition which effects patient of every age. The treatment approach of medicinal herbs has been recognized for many decades and even longer for the treatment and prevention of cold and cough. The aim of the study was to evaluate the safety and efficacy of polyherbal tablet. Polyherbal tablet containing herbs like Neem (Azadirachta indica), Tulsi (Ocimum tenuiflorum), Ginger (Zingiber officinale), Turmeric (Curcuma longa) is used for curing cold and cough. This tablet formulation reduces the healing time for sore throat and boost up the immunity. Materials and methods: The preparation of the extract and manufacturing of the polyherbal tablet is by processing technique with extract based herbal formulation and evaluation for analytical standardization including qualitative and quantitative determination were carried out. The tablets were weighed individually, the hardness and thickness were measured, friability of the tablet was measured, and the disintegration time was observed. The colour was observed to be brownish yellow and no odour. Discussion: When combining the multiple herbs in a particular ratio, it will give a better therapeutic effect and reduce the toxicity. This review mainly focusses on importance of the polyherbalism and its clinical significance. Keywords: Polyherbal tablets, formulation, evaluation, hardness, disintegration time.

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Jha, Pradeep K., Rakhi K. Jha, Dilip Rout, Sathishkumar Gnanasekar, Suresh V. S. Rana, and Maidul Hossain. "Potential targetability of multi-walled carbon nanotube loaded with silver nanoparticles photosynthesized from Ocimum tenuiflorum (tulsi extract) in fertility diagnosis." Journal of Drug Targeting 25, no. 7 (March 28, 2017): 616–25. http://dx.doi.org/10.1080/1061186x.2017.1306534.

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GEORGE, D., and P. V. SINDHU. "Effect of growing condition and harvesting method on essential oil and eugenol contents of tulsi (Ocimum tenuiflorum L.)." Journal of Crop and Weed 18, no. 2 (February 1, 2022): 30–35. http://dx.doi.org/10.22271/09746315.2022.v18.i2.1569.

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Mittal, Akanksha, Sunil Tejaswi, Mruthunjaya K, Suneeth Shetty, and Ambikathanaya UK. "Comparison of Antibacterial Activity of Calcium Hydroxide, Azadirachta Indica (Neem), Ocimum Tenuiflorum (Tulsi) and Punica Granatum (Pomegranate) Gels as Intracanal Medicaments Against Enterococcus Faecalis: An in-vitro Study." Pharmacognosy Journal 13, no. 4 (July 6, 2021): 988–94. http://dx.doi.org/10.5530/pj.2021.13.127.

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Kaur, Avneet, Yashita Jain, Kashish Mittal, Armin Kaur, and Nidhi Mittal. "ANTIOXIDANT LEVELS OF COMMON HERBS AND SPICES USED IN INDIAN TEA: BLACK PEPPER (PIPER NIGRUM), FENNEL (FOENICULUM VULGARE), CLOVE (SYGYZIUM AROMATICUM), MINT (MENTHA), CINNAMON (CINNAMOMUM VERUM) AND TULSI (OCIMUM TENUIFLORUM)." International Research Journal Of Pharmacy 9, no. 11 (December 7, 2018): 119–23. http://dx.doi.org/10.7897/2230-8407.0911270.

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Swari, Dewa Ayu Made Adnya, Ni Putu Udayana A, and Ni Made Dharma Shantini S. "STABILITY COMPARISON OF TULASI (Ocimum tenuiflorum L.) LEAF GEL HAND SANITIZER USING 0.5% AND 1% CMC-Na." Journal of Pharmaceutical Science and Application 2, no. 1 (June 18, 2020): 23. http://dx.doi.org/10.24843/jpsa.2020.v02.i01.p04.

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Background: Hand sanitizers contain 62% alcohol, softener and moisturizer. High alcohol content is able to irritate and make hands dry. A suitable moisturizer is needed for this preparation. Objective: The aim of this study is to evaluate the physical quality of gel hand sanitizer formulation of Ocimum tenuiflorum with CMC-Na as a gelling agent at a concentration of 0.5% and 1% CMC-Na. Methods: Data were collected at week-0,1,2,3,4, and replication is performed three times at each concentration of each measurement. The test results of the spread and adhesion data analysis using SPSS with a 95% confidence level. Results: Dispersive power and adhesion showed no significant difference (p <0.05) at week 0 and week 4 means second-hand sanitizer gel formulation concentration is not stable. Gel hand sanitizer leaves of Tulasi (Ocimum tenuiflorum) at a concentration of 1% CMC-Na stable from week 0 to week 3, while the concentration of 0.5% CMC-Na change at week 2. Gel hand sanitizer leaves of Tulasi (Ocimum tenuiflorum) with a gelling agent CMC-Na should need to be titrated. Conclusion: Gel hand sanitizer leaves of Tulasi (Ocimum tenuiflorum) that use concentrations of 1% CMC-Na as a gelling agent have a physical quality that is more stable than the organoleptic test concentration of 0.5% CMC-Na. Keywords: Hand sanitizer, Ocimum tenuiflorum L., CMC-Na, Physical quality evaluation

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Parida, Sagarika, Bikram Keshari Mohapatra, and Gyanranjan Mahalik. "Anatomical Study of Six Ocimum Species: The Valuable Method Used in Indian Systems of Medicines (ISM)." International Journal of Ayurvedic Medicine 11, no. 2 (July 3, 2020): 278–83. http://dx.doi.org/10.47552/ijam.v11i2.1507.

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This study aimed to characterize the morphological and anatomical traits of six Ocimum species namely Ocimum tenuiflorum L. Syn. O. Sanctum L. (green var. known as Radha Tulasi), O. tenuiflorum L. (purple var. known as Krishna Tulasi), O. basilicum L.( green var. known as Sugandhi Tulasi), O. basilicum var. pupurascens L.( purple var. known as Durlabha Tulasi), O. canum Sims. (Kanaka Tulasi) and O. gratissimum L. (Rama Tulasi). Chlorophyll estimation of young and matured leaves was also estimated. Cholorophyll a, Chl. b and total chlorophyll was maximum in both young and matured leaves of O. gratissimum L. compared to other species. Carotenoid pigments was maximum of 203.94 mg/ml in young leaves of O. gratissimum L. and 244.92 mg/ml in matured leaves of O. sanctum L. Comparative morphological characters and anatomical variation were studied for identification of the desired species in drug manufacturing industries before making formulations. This study will certainly help to ensure the quality of the crude drug as these are collected by untrained workers and supplied in dry condition to the drug manufacturing industries. Therefore, there is a chance of adulteration because of vested interest or ignorance as many plants are known by common names or in vernacular names. It was found that the six species can be identified and authenticated using these experimental findings before preparation of compound Ayurveda, Siddha and Unani (ASU) drugs in Indian System of Medicines.

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Tokala, Venkata Naga Baji. "Corrosion Inhibition of Ocimum tenuiflorum (Tulasi) Leaves Extract as a Green Inhibitor for Zinc in H2SO4." Journal of Chemistry and Chemical Sciences 8, no. 1 (January 7, 2018): 1–9. http://dx.doi.org/10.29055/jccs/546.

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Lingojwar, Sarita, Gayatri Sonawane, and Devendra Lingojwar. "Molecular identification of Indian medicinal plant tulsi (Ociumum tenuiflorum Linn.) by Internal Transcr ibed Spacer 2 region." Medicinal Plants - International Journal of Phytomedicines and Related Industries 8, no. 4 (2016): 303. http://dx.doi.org/10.5958/0975-6892.2016.00037.x.

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Jyoti, V. Vastrad, Goudar Giridhar, A. Byadgi Shameembanu, Dhanalaxmi Devi Rajkumari, and Kotur Rajashri. "Identification of bio-active components in leaf extracts of Aloe vera, Ocimum tenuiflorum (Tulasi) and Tinospora cordifolia (Amrutballi)." Journal of Medicinal Plants Research 9, no. 28 (July 25, 2015): 764–70. http://dx.doi.org/10.5897/jmpr2013.5197.

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Ajayan, Amrutha S., and N. S. Hebsur. "Green Synthesis of Zinc Oxide Nanoparticles using Neem (Azadirachta Indica) and Tulasi (Ocimum Tenuiflorum) Leaf Extract and their Characterization." International Journal of Current Microbiology and Applied Sciences 9, no. 2 (February 10, 2020): 277–85. http://dx.doi.org/10.20546/ijcmas.2020.902.035.

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B. Marathe, Sagar, Mali Naitik, and Azam Z. Shaikh. "The Therapeutic Action of Queen of Herb (Ocimum tenuiflorum)." Research Journal of Pharmacognosy and Phytochemistry, July 16, 2022, 192–94. http://dx.doi.org/10.52711/0975-4385.2022.00034.

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This review paper purpose is the ‘Queen of herb’’ means holy basil/Tulsi is how to showing it Antimicrobial activity by using different type of method. This paper also study the pharmaceutical used of Tulsi and its Pharmacological activity of Tulsi like. Antimicrobial activity, Antifungal activity, Anti-inflammatory and Analgesic activity. It is main symbol of the Hindu region tradition this is plant having unique odor found in the semitropical and tropical country. In the world. This is plant mainly used for reducing the cough. Holy basil is ancient Hindu scriptures, tulsi occupies the supreme position among the herb so that it referred to as “MOTHER”. It has been used Ayurveda and siddha practices for it’s supposed treatment of diseases. It having medicinal her that belong to the mint family and is found in 150 different worldwide. Tulsi has been used in thousands of years in Ayurveda for its diverse healing properties special about this plant is apart from being considered holy Tulsi is also reserved as great healer.

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"The Therapeutic Action of Queen of Herb (Ocimum Tenuiflorum)." American Journal of Pharmacy And Health Research 9, no. 12 (December 20, 2021). http://dx.doi.org/10.46624/ajphr.2021.v9.i12.002.

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This review paper purpose is the ‘Queen of herb’’ means holy basil/Tulsi is how to showing it Antimicrobial activity by using different type of method. This paper also study the pharmaceutical used of Tulsi & its Pharmacological activity of Tulsi like. Antimicrobial activity, Antifungal activity, Anti-inflammatory& Analgesic activity. It is main symbol of the Hindu region tradition this is plant having unique odor found in the semitropical and tropical country. In the world. This is plant mainly used for reducing the cough. Holy basil is ancient Hindu scriptures, Tulsi occupies the supreme position among the herb so that it referred to as “MOTHER”. It has been used Ayurveda and siddha practices for it’s supposed treatment of diseases. It having medicinal her that belong to the mint family and is found in 150 different worldwide. Tulsi has been used in thousands of years in Ayurveda for its diverse healing properties special about this plant is apart from being considered holy Tulsi is also reserved as great healer

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Pooja, Pooja, and Anil Kumar. "A Systemic Review of Tulsi (Ocimum tenuiflorum or Ocimum sanctum): Phytoconstituents, Ethnobotanical and Pharmacological Profile." Research Journal of Pharmacognosy and Phytochemistry, May 20, 2023, 179–88. http://dx.doi.org/10.52711/0975-4385.2023.00028.

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Ocimum tenuiflorum Linn. is an Ayurvedic herb commonly regarded as Tusli or Holy Basil. The plant has a long traditional medicinal history. In India Tulsi is worshipped by Hindus and is abundantly found in all regions. Each part of a plant like stems, roots, seeds, leaves, flowers, and fruits have their own curative properties and functions reported by many researchers. The varieties of active chemical constituents; terpenoids, phenols, flavonoids, phenylpropanoids, fatty acid derivatives, essential oil, fixed oil, and steroids are extracted from the Ocimum tenuiflorum plant and are determined of great therapeutic activities. The therapeutic actions of Tulsi compounds recreate their remedial attention and in the standardization of their medicinal results. Eugenol, Oleanolic Acid, Linalool, Ursolic Acid, Beta-Caryophyllene, Rosemarinic Acid, Estragole, Carvacrol, Methyl Cinnamate are the major compounds which are leads to various actions against diseases. This review article summarizes researchers validated various pharmacological actions of these active chemical constituents that have great potential against, antidiabetic, antioxidant, anti- inflammatory, antimicrobial, antiviral (especially against Covid-19/Coronavirus, helpful in the current scenario of “pandemic”), cardioprotective, antihypertensive, and other activities as well. This will be useful for developing new active principles and therapeutics or new life-saving drugs by researchers, practitioners, and students who were implicated in the field of ethnopharmacology.

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., Kiran, Pradeep Kumar, Simran Kirti, and Anjali Kumari. "Phytochemical Analysis of Ocimum Spp. - An Important Medicinal Plant." Current Journal of Applied Science and Technology, April 26, 2019, 1–11. http://dx.doi.org/10.9734/cjast/2019/v35i130162.

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The antioxidant activities of four genotypes of basil species were selected for biochemical studies, viz., Ocimum sanctum L. (Rama Tulsi), Ocimum tenuiflorum L. (Krishna Tulsi), Ocimum gratissimum L. (Van Tulsi), and Ocimum basilicum L. (Babui Tulsi) for the antioxidant properties. Ocimum L. is considered useful in the treatment of respiratory system disorders, reduction of blood glucose level and is regarded as an 'adaptogen' or anti-stress agent. The phytochemical analysis of the plant leaves extract to the estimation of standard biochemical methods such as peroxidase, superoxide dismutase (SOD), polyphenol oxidase (PPO), catalase activity, antioxidant, flavonoids, total phenols, protein as well as total carbohydrates, starch and reducing and non-reducing sugar. The biochemical studies revealed that the starch (98 mg/gfw), the content was found to be higher in O. gratissimum. Similarly the other parameters such as total sugar (159 mg/gfw), non-reducing sugar (61mg/gfw), Peroxidase (142 U/mL), Catalase (188 U/mL), SOD (92.47 U/mL), PPO (462 U/mL) were found to be higher in O. sanctum. On the other hand, soluble protein (62 mg/gfw), total flavonoids (325 mg/gfw) and total flavonols (486 mg/gfw) were found to be higher in O. tenuiflorum. Total antioxidants (136.45 mg/gfw), reducing sugar (108 mg/gfw) and phenol (479 mg/gfw) were found to be higher in O. basilicum. The result shows that the best genotype among the four follows the trends O. sanctum > O. basilicum > O. tenuiflorum > O. gratissimum. On the basis of the result, the two genotypes can be recommended for medicinal purpose in the pharmaceutical application.

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Patel, Jinay, and Sonia Arora. "Systematic Analysis of Compounds Present in Ocimum Tenuiflorum (Tulsi) Regarding its Anti-Inflammatory Properties Using In-Silico Techniques." Aresty Rutgers Undergraduate Research Journal 1, no. 3 (October 7, 2021). http://dx.doi.org/10.14713/arestyrurj.v1i3.173.

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The objective of this study was to gather data, create a database of the compounds present in Ocimum tenuiflorum (O. tenuiflorum), and gather related literature on the compounds found. A thor-ough literature search was performed to gather in-formation on compounds present in O. tenuiflorum, including chemical structures, relative abundance, presence in different plant parts, and availability from chemical supply vendors. The compounds’ chemical structures were refined using Discovery Studio Visualizer and Chimera software for future in-silico docking studies. The structures with cleaned structural geometry were obtained through D.S. Vis-ualizer for docking in the future. From the literature search of previously presented articles, it was found that methyl eugenol had the greatest percent com-position in O. tenuiflorum. After searching the Pro-tein Data Bank, COX-1, COX-2, and NF Kappa B were found to be the main protein targets of O. ten-uiflorum compounds in the arachidonic acid inflamematory pathway. Thus, the anti-inflammatory proper-ties of O. tenuiflorum have been analyzed in this ar-ticle for future in silico docking.

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Singh, Vikram, and Vikram Singh. "Characterizing the circadian connectome of Ocimum tenuiflorum using an integrated network theoretic framework." Scientific Reports 13, no. 1 (August 11, 2023). http://dx.doi.org/10.1038/s41598-023-40212-7.

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AbstractAcross the three domains of life, circadian clock is known to regulate vital physiological processes, like, growth, development, defence etc. by anticipating environmental cues. In this work, we report an integrated network theoretic methodology comprising of random walk with restart and graphlet degree vectors to characterize genome wide core circadian clock and clock associated raw candidate proteins in a plant for which protein interaction information is available. As a case study, we have implemented this framework in Ocimum tenuiflorum (Tulsi); one of the most valuable medicinal plants that has been utilized since ancient times in the management of a large number of diseases. For that, 24 core clock (CC) proteins were mined in 56 template plant genomes to build their hidden Markov models (HMMs). These HMMs were then used to identify 24 core clock proteins in O. tenuiflorum. The local topology of the interologous Tulsi protein interaction network was explored to predict the CC associated raw candidate proteins. Statistical and biological significance of the raw candidates was determined using permutation and enrichment tests. A total of 66 putative CC associated proteins were identified and their functional annotation was performed.

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Kanwar, Harshraj, Pradeep Singh Shekhawat, and Brajnandan Singh Chandrawat. "Effect of plant extracts against stripe rust of barley caused by Puccinia striiformis f. sp. hordei." Ecology, Environment and Conservation, February 25, 2022, 544–47. http://dx.doi.org/10.53550/eec.2022.v28i02s.082.

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Effect of plants leaf extracts, i.e. Garlic, Oak, Neem, Dathura, Partheneium, Tulsi, Castor and Ashwagandha were studied at four different concentrations (3, 5, 10 and 15 %). Barley stripe rust disease was tested on susceptible barley cultivar RD 2035 under artificial rust inoculation conditions during two consecutive Rabi, seasons 2016-17 and 2017-18 in cage house. All concentration of eight plants leaf extracts increased per cent disease suppression and decrease the per cent disease severity, than in the control. Among these extracts garlic clove extract proved as best with highest per cent disease suppression (53.10) and significantly low mean per cent rust severity (39.78) followed by leaf extracts of neem (Azadirachta indica), tulshi (Ocimum tenuiflorum), datura (Datura stramonium), oak (Calotropics gigantean), partheneium (Parthenium hysterophorus), castor (Ricinus communis) and ashwagandha (Withania somnifera).

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SAHU, SARIKA, and BHAGYASHREE DESHPANDE. "QUALITATIVE ESTIMATION OF PHYTOCHEMICALS IN DIFFERENT OCIMUM SPECIES OF CHHATTISGARH." Asian Journal of Microbiology, Biotechnology and Environmental Sciences, June 30, 2022, 341–45. http://dx.doi.org/10.53550/ajmbes.2022.v24i02.021.

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Medicinal plants and herbs have been proved to be of great importance to the health of theindividuals and communities. The potential for developing antibacterial from higher plants appearsrewarding as it will result to the development of a phytomedicine to act against microbes. Phytochemicalscreening of Tulsi plant (Ocimum sp.) was studied in the present investigation. Phytochemical analysis ofmethanol and aqueous extracts of O. tenuiflorum, O. gratissumand O. sanctum demonstrated the presenceof phytoconstituents like Tannins, Alkaloids, Saponins, Glycosides, Terpenoids, Flavonoids, Phenols,Carbohydrates, Steroids, and Anthraquinones. Presence of such phyto constituents will increase interestwith the mankind to develop anti-microbial drugs using different species of Oscimum for varied microbes.

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Ambujavalli, J., K. Santhiya, K. Rohini, and V. Kumaresan. "Assessment of Growth Characteristics of Medicinal Plants Ocimum tenuiflorum L. and Plectranthus amboinicus (Lour.) Spreng. Under Shade Net and Open Conditions." European Journal of Medicinal Plants, January 4, 2020, 1–8. http://dx.doi.org/10.9734/ejmp/2019/v30i430186.

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Aim: To study the effect of shade net on the growth characteristics of two medicinal plants viz., Tulsi and Indian Borage in terms of plant height, number of leaves, leaf area and flowering in relation to open condition. Place and Duration of Study: Lawspet, Puducherry, India between June 2018 and October 2018. Methodology: Tulsi and Indian Borage plants were grown in similar type of soil. The potted plants were kept in open, 35% and 75% shade net conditions and studied for 3 months. The plant height, number of leaves, and leaf area were measured including number of inflorescence branches for Tulsi. Results: The average air temperature was lower under shade net. Relative humidity was more in shade net conditions with maximum in 75% shade and least in open conditions. The number of leaves of Tulsi and Indian Borage recorded was maximum when grown under 35% shade and least in open conditions. Plant height was maximum in 75% shade for Tulsi and 35% shade in Indian Borage and least in open conditions on both the cases. Leaf area was maximum for both Tulsi and Indian Borage in 75% shade. Conclusion: Growth of the two medicinal plants was influenced by the microclimate conditions. The present study shows that the medicinal plants can be grown in shade net conditions to increase the yield of medicinally useful parts.

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Girme, Aboli, Prajkta Bhoj, Ganesh Saste, Sandeep Pawar, Amit Mirgal, Dipak Raut, Machindra Chavan, and Lal Hingorani. "Development and Validation of RP-HPLC Method for Vicenin-2, Orientin, Cynaroside, Betulinic Acid, Genistein, and Major 8 Bioactive Constituents with LC-ESI-MS/MS profiling in Ocimum genus." Journal of AOAC INTERNATIONAL, April 30, 2021. http://dx.doi.org/10.1093/jaoacint/qsab067.

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Abstract Background Ocimum genus known as Tulsi or Basil is a prominent botanical class in Asian culture, especially in India. The leaves have an immunomodulatory, antioxidant, stress-relieving, and adaptogenic role in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals. Objective The HPLC-PDA method was developed and validated for vicenin-2, orientin, cynaroside, betulinic acid, genistein, with syringic acid, rosmarinic acid, eugenol, carnosic acid, oleanolic acid, ursolic acid, luteolin, apigenin for quantification and confirmed using a novel ESI -MS/MS method in the Ocimum samples. Method The methodology parameters were developed on the RP-C18 column with a gradient elution of 1 mL/min flow rate for 0.1% o-phosphoric acid and acetonitrile at 210 and 340 nm wavelengths. Result The validation data for 13 bioactive compounds showed good linearity (r2> 0.99) with sensitive LOD (0.034-0.684 µg/mL) and LOQ (0.100-2.068 µg/mL) with recoveries (83.66-101.53%).The results were found precise (RSD,<5.0%) and accurate (RE, -0.60-1.06) for the quantification. The method performance was verified and found robust by analyzing ten samples of O. tenuiflorum from the ten geographical states of India (RSD, < 5.0%). Conclusion The validated HPLC-PDA method was found selective and suitable for analyzing thirteen compounds in O. tenuiflorum and twelve cultivars from the Ocimum genus as a quality control tool. This method can be used in routine analysis as an inexpensive alternative to advanced techniques. Highlights This work is the first to report for vicenin-2, orientin, cynaroside, betulinic acid, and genistein, with simultaneous analysis of eight bioactive compounds in the Ocimum genus.

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Sahu, Pravin Kumar, Suryakant Chakradhari, Khageshwar Singh Patel, Jesús Martín-Gil, Erick K. Towett, and Pablo Martín-Ramos. "Nutritional, Spectral and Thermal Characteristic of Lamiaceae Seeds." European Journal of Medicinal Plants, July 17, 2019, 1–13. http://dx.doi.org/10.9734/ejmp/2019/v28i330133.

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Aims: Species of the family Lamiaceae possess a rich tradition of use for flavoring and medicinal purposes. This paper focusses on the nutritional and thermal characteristics of the seeds from eight species belonging to this family: Gmelina arborea Roxb. ex Sm., Hyptis suaveolens (L.) Poit., Leonotis nepetifolia (L.) R.Br., Ocimum americanum L., Ocimum sanctum L. (Rama Tulsi), Ocimum tenuiflorum L. (Krishna Tulsi), Origanum vulgare L. and Tectona grandis L.f. Methodology: The oil, starch, total polyphenol, flavonoid and mineral contents for aforementioned seeds were determined. Fourier-transform infrared (FTIR) spectroscopy was used to assess the phytoconstituents. Thermogravimetric/derivative thermogravimetric analyses (TG/DTG) and differential scanning calorimetry (DSC) analyses were performed to analyze the decomposition patterns. Results: The concentrations of oil, starch, total polyphenol, flavonoids and minerals for the seeds from the eight plants under study ranged from 11.8 to 50.4%, from 0.22 to 1.84%, from 295 to 5842 mg/kg, from 1660 to 12680 mg/kg and from 11756 to 33927 mg/kg, respectively. Unsaturated oils, polyphenols and lignin were recognized by vibrational spectroscopy. The sequence of thermal effects in the seed pyrolysis process above 100°C have been put in relation to seed protein crystallization (endotherm at 200°C), oxidation reactions and degradation of hemicellulose and other fiber components (at around 300°C), and decomposition of polyunsaturated (at 357°C) and mono-unsaturated (at 391°C) triglycerides. Conclusion: Lamiaceae seeds are potential food alternatives to cereals.

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Hymavathi, Atmakur, Sohail Nizamuddin, and Himabindu Bantikatla. "Design, green synthesis and characterization of zinc oxide nanoparticles from Ocimum tenuiflorum Var. Ram tulsi." Materials Today: Proceedings, September 2021. http://dx.doi.org/10.1016/j.matpr.2021.08.344.

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Upadhyay, Atul K., Anita R. Chacko, A. Gandhimathi, Pritha Ghosh, K. Harini, Agnel P. Joseph, Adwait G. Joshi, et al. "Genome sequencing of herb Tulsi (Ocimum tenuiflorum) unravels key genes behind its strong medicinal properties." BMC Plant Biology 15, no. 1 (August 28, 2015). http://dx.doi.org/10.1186/s12870-015-0562-x.

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Pandey, Vivek Kumar, Siddh Nath Upadhyay, and Pradeep Kumar Mishra. "Light-induced synthesis of silver nanoparticles using Ocimum tenuiflorum extract: Characterisation and application." Journal of Chemical Research, June 29, 2020, 174751982093651. http://dx.doi.org/10.1177/1747519820936511.

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Abstract:

Stable silver nanoparticles of various sizes are synthesised using the aqueous extract of Tulsi ( Ocimum tenuiflorum) as the reducing agent as well as a stabilising agent under the influence of light of wavelength ranging from 200 to 900 nm. The formation of silver nanoparticles is monitored using a UV-Visible spectrophotometer and the particle shape, size and stability are analysed using scanning electron microscopy, dynamic light scattering and zeta potential measurement techniques. It is observed that the size of the silver nanoparticles decreases on increasing the wavelength of irradiating light. The silver nanoparticles show antimicrobial activity against gram-positive ( Bacillus subtilis), as well as gram-negative ( Escherichia coli) bacteria. The antimicrobial activity shows an inverse relationship with the size of the silver nanoparticles. The bio-synthesised silver nanoparticles are successfully embedded in a polymeric film to act as an active antimicrobial film that can potentially release the active constituents.

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Journal articles on the topic 'TULSI(OCIMUM TENUIFLORUM)'

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