Dissertations / Theses on the topic 'Tribologie orale in vitro'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 25 dissertations / theses for your research on the topic 'Tribologie orale in vitro.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Ammam, Ianis. "Études et caractérisations tribologiques des mécanismes biophysiques de la lubrification orale." Electronic Thesis or Diss., Ecully, Ecole centrale de Lyon, 2024. http://www.theses.fr/2024ECDL0042.
Full textThe study of oral lubrication has become a current concern for the food industry. Quantitative analyses allow for understanding and anticipating physiological mechanisms, such as predicting astringency phenomena in food products. Astringency is characterized by a decrease in oral mucosa lubrication following the consumption of plant-based products. However, current research on oral lubrication relies on synthetic materials that poorly represent oral tissues, neglecting interactions between secreted salivary proteins and transmembrane proteins, thus limiting the understanding of lubrication mechanisms. This thesis is part of the MACARON project, which aims to investigate the role of oral mucosa in sensory perception. In vitro models of oral mucosa expressing the transmembrane protein MUC1 have been developed to simulate fundamental interactions between MUC1 and salivary proteins responsible for tissue lubrication and hydration. Additionally, a tribometer has been designed to perform in vitro tribological tests on these epithelial models to monitor their lubrication state. Thus, this thesis focuses on studying the molecular mechanisms of oral lubrication through an in vitro tribological approach, using macro- and micrometric physical parameters. Firstly, this manuscript provides a study on the crucial role of MUC1 mucin and its structure in oral lubrication. The presence of MUC1 enhances lubrication by improving the retention of salivary proteins on the cell surface. Secondly, the manuscript explores molecular mechanisms of astringency. In vitro tribological tests in the presence of astringent compounds show that these substances form aggregations on the epithelial surface, reducing oral lubrication. Concurrently, our work demonstrates protective mechanisms, including the dissociation of MUC1 and the interaction of proline-rich proteins with tannins, mitigating these adverse effects on lubrication. Through additional study, correlations between sensory perception and our measured physical properties are established, demonstrating the ability of our methodology to classify individuals based on their sensitivity to astringency. Finally, the last study presents the development of a new oral mucosa model aiming to reproduce mechanical and physicochemical properties of in vivo mucosa. This thesis proposes an innovative methodology for studying oral lubrication, particularly focusing on mechanisms responsible for astringency sensation through the use of mucosa models increasingly closer to physiological oral tissues
DI, FELICIANTONIO MARINA. "Studio in vitro sulla stabilità strutturale dei cannabinoidi sintetici nel fluido orale." Doctoral thesis, Università Politecnica delle Marche, 2018. http://hdl.handle.net/11566/253005.
Full textIn the last decade the illegal drug market has seen the birth, first, and the affirmation, then, of synthetic substances much more dangerous than traditional drugs. In 2012 the Commission on Narcotic Drugs classified them under the term NPS or "New Psychoactive Substances", legally marketed as environmental fragrances named "Spice". Chemical-toxicological results have highlighted the presence, in this products, of synthetic cannabinoids particularly affine to CB1 cannabinoid receptors. The aim of the research was to evaluate the structural stability of these molecules when subjected to a change of the physical state due to the high temperatures reached during the smoking process. The attention was drawn to saliva, the biological matrix immediately involved in the smoking process, characterized by non-invasive sampling and which allowed obtaining reliable and repeatable data in high-resolution mass spectrometry. The highly lipophilic structure of the molecules requires the use of glass containers, in order to avoid adsorption on the surface of polypropylene tubes, while the storage temperature to which the biological matrix is subjected influences the potential degradation of synthetic cannabinoids by salivary and/or microbial enzymes. The results obtained show a certain structural stability of the molecules under investigation, but the analytical data obtained, usable both in the clinical and forensic field, must comply with a strict analytical/instrumental protocol.
Caisson, Armelle. "Mise au point d'un modèle "in vitro" permettant de reproduire la pharmacocinétique d'un antibiotique après administration orale." Paris 5, 1991. http://www.theses.fr/1991PA05P075.
Full textPerrichon, Armelle. "Tribologie et vieillissement de prothèses totales de hanche en biocéramique, in vitro = in vivo ? Enjeux scientifique et sociétal." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSEM002/document.
Full textThe performance of the prosthetic couple (femoral head/cup) influences strongly the lifetime of a total hip prosthesis. Ceramics exhibit an excellent combination of biocompatibility, mechanical resistance and tribological properties. Zirconia toughened alumina (ZTA) composites are made of an alumina matrix and well dispersed zirconia particles. They are tailored in order to offer the best compromise of hardness, chemical stability, toughness and mechanical resistance. The prediction of the performance of such materials must take into consideration the three main sources of degradation identified for Ceramic-on-Ceramic (CoC) bearings: shocks due to micro-separation, friction and low temperature degradation (LTD). Experimental tests on a shock machine, a hip-walking simulator and in an autoclave are able to simulate in vitro each of these sources of degradation, respectively. The aim of the project is to combine these tests in order to solve better the equation in vitro = in vivo. Shocks dominate the processes of degradation with the formation of wear stripes. The tested material showed an excellent resistance to LTD. The damage induced by the standard wear test on a hip-walking simulator is negligible, which raises the question about the relevance of this test for CoC couplings. An analysis of explants confirmed the decisive role of shocks in experimental tests. Zirconia phase transformation was mechanically induced within both in vitro and in vivo wear stripes. A degradation mechanism was suggested within the stripes. The material response is partly determined by the force applied during shocks
METO, AIDA. "Approcci innovativi per studi sui patogeni del cavo orale: modelli di studio in vitro ed ex vivo." Doctoral thesis, Università degli studi di Modena e Reggio Emilia, 2021. http://hdl.handle.net/11380/1246163.
Full textDuring recent years, novel compounds/tools are being proposed to maintain oral health and/or to treat dental/periodontal problems. As well known, dental caries are among the most diffused infections and their improper management turns towards relevant disease(s) and eventually tooth extraction. Extensive literature documents the pathogenic role of certain microorganisms and their ability to persist in the oral cavity, as a complex microbial community, including bacteria, viruses and fungi, tightly enclosed in a polymeric matrix of polysaccharide origin. Such sessile community, and particularly dental plaque, the first deeply studied human-associated biofilm, is notoriously refractory not only to common cleaning procedures by mouthwashes and tooth-pastes/brushes, but also to antimicrobial drugs and host immune defenses. This scenario becomes further complicated considering that the widely diffused orthodontic treatments, with fixed or removal brackets, extend the clinical challenge, being such devices an additional good habitat for microbial adhesion, growth and biofilm formation. To a similar extent, patients with dental implants may locally develop biofilm-related diseases, allowing clinical progression toward pathogen-related peri-mucositis or peri-implantitis. From here, the need arises for innovative tools/compounds to facilitate microbial removal and maintenance of oral cavity homeostasis. Besides the most investigated oral pathogens, including Streptococcus mutans-group and the “red complex” Gram-negative anaerobe bacilli, also Candida albicans (C. albicans), Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) may occur as causative agent of oral diseases. The first, often harbored as commensal of healthy mucosae, is the main fungal pathogen involved in oral mucositis. The latter two are subtle pathogens, responsible of wide-spectrum diseases; they are being extensively used for in vitro studies, because of their numerous virulence factors and wide-spectrum antimicrobial resistance. The aim of the present thesis was to evaluate in vitro and ex vivo, the antimicrobial and antibiofilm efficacy of innovative approaches against oral pathogens. Our data provided in vitro and ex vivo evidence on the antimicrobial efficacy of several dental-care compounds. A novel use of the endodontic product Cupral could be proposed in daily hygiene practices. The Bic-40 treatment was shown as the best approach in cleaning smooth and rough titanium surfaces (without altering their properties); importantly, its device-decontamination efficacy did not affect the biological properties of reparative stem cells. Furthermore, our work added new insights on the anti-microbial properties of a natural compound, such as propolis, and on its possible mechanisms of action. At last, we showed that the Biorepair Peribioma toothpaste and gum deeply affected oral microorganisms’ behavior, drastically impairing their ability to contaminate and produce plaque onto orthodontic devices; interestingly, replacement by beneficial microorganisms was observed. The overall take-home message from this research is that basic science may greatly increase our knowledge on how to counteract biofilm-producing pathogens; in turn, this will facilitate prevention and/or treatment of dental and oral biofilm-associated infections, making a huge difference in terms of health promotion.
Reix, Nathalie. "Administration orale d'insuline : validation in vitro et in vivo d'un système basé sur une double encapsulation de l'insuline." Strasbourg, 2009. http://www.theses.fr/2009STRA2495.
Full textThe purpose of this study was to develop a new formulation of oral insulin. Normally, peptide hormones like insulin are given by parenteral injections routes because they are destroyed by the acid and proteolytic enzymes in stomach and intestine. Our project focuses on insulin double encapsulation for oral administration. The first system of encapsulation of insulin is based on Poly(Lactide-co-Glycolide) Acid (PLGA) nanoparticles. This should allow the uptake and the transport across mucosal intestinal barrier. These nanoparticles are put in a gastroresistant capsule made of Eudragit® L100-55. After synthesis, the size of nanoparticles is 165 ±4 nm and the rate of encapsulation is around 95%. In vitro, the absorption has been quantify by flow cytometry and visualized by confocal microscopy. A clathrin dependant endocytosis pathway mechanism has been demonstrated. In vivo, the biofunctionnality of the nanoparticles was evaluated after subcutaneous injections. After nanoparticles gastric force feeding no effect was seen on the glycemia because nanoparticles are not gastroresistant. We measured the kinetic of the glycemia and the biofunctionnality of NP in streptozotocin-induced diabetic rats after intra-duodenal injections of insulin nanoparticles and intraperitoneal injections of insulin. The bioavaibility was evaluated after quantification of the C-peptidemia after intra-peritoneal injections of C-peptide and after intraduodenal injections of the same quantity of encapsulated C-peptide. Intraduodenal injections of insulin nanoparticles induce a significant decrease of glycemia 8h after injection. In comparison with intra-peritoneal injections, results showed that nanoparticles’ biofunctionnality is at less 20% and the biodisponibility is 6,3%. PLGA insulin-loaded nanoparticles are efficient and the biological effect of insulin is preserved. These polymeric particles allow the absorption of insulin through intestinal mucosa into the bloodstream. Thus this new delivery insulin formulation seems to be an interesting approach
Senft, Nicole [Verfasser], Sigrun [Akademischer Betreuer] Eick, Wolfgang [Akademischer Betreuer] Pfister, and Arndt [Akademischer Betreuer] Günsch. "In-vitro-Studie zur antibakteriellen Wirkung von Kiefernkernholz-Extrakt auf orale Mundspezies / Nicole Senft. Gutachter: Sigrun Eick ; Wolfgang Pfister ; Arndt Günsch." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2012. http://d-nb.info/1028236875/34.
Full textBroos, Katharina [Verfasser], and Karin Christine [Akademischer Betreuer] Huth. "Wirkung von verschiedenen hydraulischen Silikatzementen auf orale Zellen in vitro : Biokompatibilität, Entzündungsregulation und regeneratives Potential / Katharina Broos ; Betreuer: Karin Christine Huth." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/113892265X/34.
Full textArien, Albertina. "Étude in vitro et in vivo de la stabilité de liposomes contenant de la calcitonine." Bordeaux 2, 1995. http://www.theses.fr/1995BOR28364.
Full textKhelaifia, Saber. "Détection et culture des archaea associées aux muqueuses intestinale et orale humaines." Thesis, Aix-Marseille, 2013. http://www.theses.fr/2013AIXM5029.
Full textArchaea is one of four known domains of life. Unlike what their name suggests, they some species of methanogenic archaea have been associated with oral, vaginal and intestinal mucosa. These methanogenic archaea are obligate anaerobic prokaryotes and their culture conditions are fastidious and very poorly known. Only four methanogenic archaea have been isolated from human samples including the digestive microbiota; Methanobrevibacter smithii detected in 95.7% of individuals Methanosphaera stadtmanae found in approximately one third of individuals and more recently in our laboratory Methanomassilicoccus luminyensis detected on average in 4% of individuals with a prevalence of age-related, and in the oral microbiota Methanobrevibacter oralis isolated from dental plaque
Forstner, Teresa [Verfasser], and Karin Christine [Akademischer Betreuer] Huth. "Untersuchung des Einflusses von neuen endodontischen Sealern auf orale Zellen in vitro: Toxizitätsanalysen, Entzündungskaskade und mögliches regeneratives Potential / Teresa Forstner ; Betreuer: Karin Christine Huth." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1148940472/34.
Full textMorell, Esteve Pere. "Diseño de alimentos lácteos con capacidad saciante. Relación entre estructura, procesamiento oral y percepción." Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/89093.
Full textLa presente tesis estudia cómo aumentar las expectativas de la capacidad saciante en productos lácteos analizando el efecto de la incorporación de determinados ingredientes. El estudio de la reformulación de alimentos con alta expectativa sobre su capacidad saciante contribuye a moderar la elección de las raciones de alimentos y a regular la ingesta. En la presente tesis se han estudiado principalmente dos de los factores que más influyen en la percepción de capacidad saciante de alimentos semisólidos: a) el aumento en el tiempo de procesado oral que produce saciedad debido a una prolongación de las señales orosensoriales, y b) el alto contenido en proteínas. El aumento del tiempo de procesado oral lo producen, por ejemplo, mayores consistencias, recubrimiento oral o sensaciones de cremosidad, que son características que se pueden modificar por adición de hidrocoloides. Se emplearon almidón nativo y modificado, goma guar y ¿-carragenato para aumentar la viscosidad y consistencia de batidos lácteos. La caracterización reológica de los productos reveló patrones de comportamiento muy diferentes dependiendo del tipo de hidrocoloide. La adición de la saliva en los estudios demostró modificar las percepciones sensoriales, por lo que su inclusión resulta recomendable y más realista. Se estudió también la adición de hidroxipropilmetilcelulosa (HPMC) y el efecto de dos componentes normales en productos lácteos (sólidos lácteos y nata). Además del efecto dominante del HPMC en la temporalidad de las percepciones, la presencia de glóbulos de grasa o sólidos de la leche modularon dichas percepciones. Para aumentar el contenido en proteína se seleccionó como alimento el yogur, al que se añadió el doble de la cantidad de proteína de distintas fracciones lácteas y almidón. El yogur con el doble de leche en polvo fue globalmente el más aceptado y más cercano a las características "ideales" según los consumidores, con una serie de atributos sensoriales que contribuyen a la sensación de saciedad esperada. Por otro lado, la digestión oral in vitro mostró que los gránulos de almidón procedentes de almidón físicamente modificado permanecen inalterados después del ataque de la ¿-amilasa de la saliva, y serían los responsables de la mayor consistencia y cremosidad. Adicionalmente, se encontró que la sensación de astringencia resultaba negativa; los estudios tribológicos permitieron interpretar las características sensoriales. Las propiedades lubricantes de algunas muestras no reflejaron la diferencia de astringencia sensorial indicando que ésta no era una percepción puramente táctil causada por un aumento en la fricción. La adición de almidón modificado físicamente redujo significativamente los valores de los coeficientes de fricción relacionados con los polímeros de almidón solubles y la conservación de gránulos en el almidón. Todas las estrategias abordadas permitieron comprender y ahondar en los conocimientos que conducen a cómo reformular un alimento para aumentar la percepción de saciedad. Dichas estrategias son extrapolables a otras categorías de alimentos lo que amplía el alcance de los resultados obtenidos en la presente tesis.
La present tesi estudia com augmentar les expectatives de la capacitat saciant en productes lactis analitzant l'efecte de la incorporació de determinats ingredients. L'estudi de la reformulació d'aliments amb alta expectativa sobre la seua capacitat saciant contribueix a moderar l'elecció de les racions d'aliments i a regular la ingesta. En la present tesi s'han estudiat principalment dos dels factors que més influeixen en la percepció de capacitat saciant d'aliments semisòlids: a) l'augment en el temps de processat oral que produeix sacietat a causa d'una prolongació dels senyals orosensorials, i b) l'alt contingut en proteïnes. L'augment del temps de processat oral el produeixen, per exemple, majors consistències, recobriment oral o sensacions de cremositat, que són característiques que es poden modificar per addició d'hidrocol·loïdes. S'emprà midó natiu i modificat, goma guar i ¿-carragenat per augmentar la viscositat i consistència de batuts lactis. La caracterització reològica dels productes revelà patrons de comportament molt diferents depenent del tipus d'hidrocol·loïde. L'addició de la saliva en els estudis demostrà modificar les percepcions sensorials, per la qual cosa la seua inclusió resulta recomanable i més realista. S'estudià també l'addició d'hidroxipropilmetilcel·lulosa (HPMC) i l'efecte de dos components normals en productes lactis (sòlids lactis i nata). A més de l'efecte dominant del HPMC en la temporalitat de les percepcions, la presència de glòbuls de greix o sòlids de la llet van modular les citades percepcions. Per augmentar el contingut en proteïna es va seleccionar com a aliment el iogurt, a què s'afegí el doble de la quantitat de proteïna de diverses fraccions làcties i midó. El iogurt amb el doble de llet en pols fou globalment el més acceptat i més pròxim a les característiques "ideals" segons els consumidors, amb una sèrie d'atributs sensorials que contribueixen a la sensació de sacietat esperada. D'altra banda, la digestió oral in vitro mostrà que els grànuls de midó procedents de midó físicament modificat romanen inalterats després de l'atac de l'¿-amilasa de la saliva, i serien els responsables de la major consistència i cremositat. Addicionalment, es trobà que la sensació d'astringència resultava negativa; els estudis tribològics permeteren interpretar les característiques sensorials. Les propietats lubricants d'algunes mostres no reflectiren la diferència d'astringència sensorial indicant que esta no era una percepció purament tàctil causada per un augment en la fricció. L'addició de midó modificat físicament reduí significativament els valors dels coeficients de fricció relacionats amb els polímers de midó solubles i la conservació de grànuls en el midó. Totes les estratègies abordades permeteren comprendre i aprofundir en els coneixements que condueixen a com reformular un aliment per augmentar la percepció de sacietat. Les citades estratègies són extrapolables a altres categories d'aliments, cosa que amplia l'abast dels resultats obtinguts en la present tesi.
Morell Esteve, P. (2017). Diseño de alimentos lácteos con capacidad saciante. Relación entre estructura, procesamiento oral y percepción [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/89093
TESIS
De, Bortoli Marco. "Studio della tossicità da palitossina e composti analoghi mediante modelli in vitro e in vivo." Doctoral thesis, Università degli studi di Trieste, 2011. http://hdl.handle.net/10077/4502.
Full textLa Palitossina (PLTX), una delle biotossine marine più tossiche finora note, è saltata agli onori della cronaca in seguito al suo frequente rilevamento in campioni di una microalga tropicale, Ostreopsis ovata, ormai diffusa anche in Mar Mediterraneo, dove sono stati segnalati più volte problemi respiratori in concomitanza alla sua presenza. La tossina è stata rilevata anche in molluschi ed altri prodotti ittici, che possono fungere da vettori per l’ultimo anello della catena alimentare, l’uomo. Poiché in paesi tropicali sono stati segnalati casi di intossicazione gravi, anche letali, in seguito all’ingestione di pesci e crostacei contaminati con PLTXs, si rende necessario monitorare la presenza di questi composti nei prodotti ittici e/o nelle microalghe produttrici, anche in assenza di una legislazione in merito. All’inizio di questo lavoro erano disponibili solo pochi dati relativi alla tossicità acuta di questo composto, spesso purificato con protocolli non perfezionati. Poiché anche i dati clinici disponibili non permettevano un’esatta definizione dell’Acute Reference Dose (ARfD), necessaria per determinare i livelli massimi di tossina ammissibili nei prodotti ittici, si è deciso inizialmente di studiare la tossicità acuta della PLTX (e di un analogo 42-OH-PLTX) dopo somministrazione orale nel topo. I sintomi e le analisi cliniche condotte sui topi hanno indicato un coinvolgimento del sistema neuromuscolare. Questo studio, insieme ad altri pubblicati nel frattempo, hanno permesso agli esperti dell’EFSA di definire la concentrazione di 30 μg di tossina per Kg di polpa di molluschi quale livello al di sopra del quale si possano manifestare effetti tossici nell’uomo. Si è proceduto poi alla messa a punto di due saggi per la determinazione di questi composti: un saggio strutturale di tipo ELISA ed uno funzionale, il saggio emolitico. Il saggio ELISA (sandwich indiretto) è stato messo a punto utilizzando l’anticorpo monoclonale 73D3, e un anticorpo policlonale di coniglio prodotto presso l’Università di Trieste. Il saggio rileva la PLTX in un range di concentrazioni che vanno da 1,25 a 40 ng/ml ed è in grado di quantificare con la stessa sensibilità anche la 42-OH-PLTX, isolata e caratterizzata dal punto di vista chimico durante questo periodo di dottorato dal gruppo del prof. E. Fattorusso (Università di Napoli Federico II), in un campione di palitossina gentilmente fornitoci dal dr. M. Poli (Maryland, USA). Il saggio ELISA è in grado di rilevare anche l’Ostreocina-d, un altro analogo della PLTX, ma a concentrazioni maggiori rispetto a quelle della PLTX (³40 ng/ml). Il mancato rilevamento di acido okadaico, acido domoico, brevetossina-3, saxitossina e yessotossina (tossine che possono essere presenti insieme alla PLTX nei prodotti ittici contaminati) indica la specificità del saggio. Siamo poi passati alla messa a punto del saggio emolitico, ampiamente usato in letteratura per il rilevamento di PLTX e di composti palitossino-simili. Questo saggio sfrutta la capacità della tossina di indurre emolisi tardiva probabilmente tramite l’alterazione della Na+/K+-ATPAasi (NAKA). In letteratura, però, non è disponibile un protocollo standardizzato e la variabilità dei risultati riportati è notevole. Si è pertanto proceduto a realizzare il saggio emolitico, esplorando le variabili che ne influenzano la performance, ottenendo una EC50 = 13,2 pM per la PLTX. Gli anticorpi monoclonale e policlonale anti-PLTX hanno inibito con equa potenza l’emolisi indotta da PLTX e possono quindi essere usati per verificare la specificità dell’emolisi in campioni incogniti. Dopo aver verificato che anche la 42-OH-PLTX condividesse lo stesso recettore della PLTX mediante un saggio di binding indiretto alla NAKA (EC50 di 28.2 nM e 29.4 nM rispettivamente per 42-OH-PLTX e PLTX), è stato eseguito il saggio emolitico anche sulla 42-OH-PLTX, ottenendo dei risultati analoghi (EC50 = 7.6 pM) a quelli della PLTX. Nell’ottica di un utilizzo di questo saggio in situazioni di monitoraggio si è valutata la possibilità di ridurre i suoi tempi di esecuzione e in tal senso, cambiando la concentrazione salina della soluzione tampone al 62 % di quella normale, si è riusciti a ridurre il tempo di incubazione di 4 volte (1 h anziché 4 h). La curva concentrazione-risposta ottenuta dopo incubazione di 1 h con la PLTX in tampone al 62 % è risultata perfettamente sovrapponibile a quella ottenuta dopo 4 h di incubazione della tossina in tampone 100%. Al contrario, nessuna delle concentrazioni di PLTX testate ha dato emolisi dopo incubazione di 1 h della tossina in tampone 100%. Questo aspetto è particolarmente interessante perché permetterebbe di distinguere l’emolisi dovuta a palitossina da una emolisi aspecifica, semplicemente conducendo il saggio in 1 ora in parallelo nei due tamponi 62 % e 100 %, evitando l’uso di anticorpi anti-PLTX. In particolare, nel caso di un campione ignoto che dia emolisi in PBS al 62 % e non in PBS al 100 %, il risultato fornirebbe un primo indizio della presenza di palitossina, da confermare con metodi di riferimento (LC-MS). Se invece l’emolisi avviene ad entrambe le concentrazioni di PBS, dopo incubazione per 1 ora, essa potrebbe dipendere da un’azione aspecifica non imputabile alla sola palitossina. La presenza di una proliferazione massiccia (6.700.000 di cellule/litro) di Ostreospis cf. ovata nel Golfo di Trieste, ci ha permesso di utilizzare gli anticorpi monoclonale e policlonale per la localizzazione immunocitochimica delle tossine nelle singole cellule di microalghe. Per la prima volta è stata così visualizzata la presenza delle palitossine in cellule di Ostreopsis cf. ovata, che risultano distribuite in tutto il citoplasma. La positività per le tossine è stata verificata in tutte le cellule di Ostreopsis analizzate, mentre nessuna cellule di Coolia monotis osservate è risultata positiva, a conferma della specificità verso la PLTX del segnale degli anticorpi. L’analisi HR LCMS ha evidenziato la presenza di ovatossine-a, -b, -c, -d/-e, con una forte prevalenza di ovatossinaa (circa 80%, 45-64 pg/cellula), mentre per la prima volta in un campione naturale non è stata rilevata la presenza di PLTX. Questi risultati ci hanno permesso di concludere che entrambi gli anticorpi utilizzati sono in grado di riconoscere anche le ovatossine, analoghi della palitossina preponderanti nel Mar Mediterraneo. Inoltre, la tecnica immunocitochimica eseguita direttamente sulle microalghe potrebbe permettere un’allerta precoce della presenza di palitossine, ad esempio prima del loro ingresso/accumulo nella catena alimentare, evitando eventuali problemi per la salute pubblica. Un altro approccio per il rilevamento della tossina è stato fatto utilizzando la spettroscopia Raman. La palitossina (il cui spettro Raman è stato qui registrato per la prima volta) è stata ricercata in singole cellule di Ostreospis, depigmentate con acetone-esano 1:1. Non sono stati riscontrati segnali univocamente attribuibili alle palitossine negli spettri Raman di Ostreopsis, probabilmente a causa della loro uniforme diffusione citoplasmatica, come visualizzato in immunocitochimica. Nelle cellule non depigmentate con acetone:esano 1:1 è stata confermata le presenza del carotenoide peridinina. L’analisi Raman di cellule in coltura di Ostreopsis cf. ovata nelle diverse fasi di crescita ha evidenziato forti segnali associabili ad acidi grassi polinsaturi, già riscontrati in Ostreopsis cf. ovata con altre tecniche. L’analisi HR LC-MS delle cellule in coltura nelle varie fasi di crescita ha mostrato, analogamente alle relative popolazioni naturali, un elevato contenuto di ovatossina-a (circa 55%, 7.5–19.7 pg/cellula) e minori quantità di altre ovatossine, con la palitossina presente in tracce (< 0,1 pg/cellula). Si è osservato che il contenuto di tossine aumenta con l’età della coltura, con le cellule in fase senescente (giorno 25 dall’avvio della coltura) contenenti circa il doppio di tossina delle cellule in fase stazionaria (giorno 18). Quindi, analogamente a quanto si verifica per altri metaboliti secondari negli organismi vegetali, l’accumulo di queste tossine raggiunge il massimo generalmente verso la fine del ciclo vitale.
XXIII Ciclo
1983
Ménez-Berlioz, Cécile. "Intéractions de la miltefosine avec la barrière intestinale : étude des mécanismes de capture et de transport sur un modèle in vitro de cellules CACO-2 : application à la conception d'une bithérapie antileishmanienne." Paris 11, 2006. http://www.theses.fr/2006PA114834.
Full textMiltefosine (hexadecylphosphocholine, HePC) has recently been developed for the treatment of visceral leishmaniasis. It has the particular advantage of being active after oral administration. The oral route is preferable because it is non invasive, is often less expensive and favours compliance with treatment. There is not much information in the literature about the passage of miltefosine across the intestinal barrier. Therefore, the aims of this thesis were to study the effects of miltefosine on the intestinal epithelium and to determine the mechanisms involved in its uptake and transport, using a model of Caco-2 cells in culture. The results were applied to the development of antileishmanial bitherapy by the oral route: the ability of miltefosine to improve the oral bioavailability of another antileishmanial agent, amphotericin B, was evaluated
Twarog, Caroline. "Comparison of the intestinal permeation enhancers, SNAC and C₁₀, for oral peptides : biophysical, in vitro and ex vivo studies Intestinal Permeation Enhancers for Oral Delivery of Macromolecules: A Comparison between Salcaprozate Sodium (SNAC) and Sodium Caprate A head-to-head Caco-2 assay comparison of the mechanisms of action of the intestinal permeation enhancers: SNAC and sodium caprate (C10) Comparison of the mechanisms of action of the intestinal permeation enhancers, SNAC and sodium caprate (C10): isolated rat intestinal mucosae and sacs." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASS045.
Full textSodium caprate ((C₁₀) and salcaprozate sodium (SNAC) are permeation enhancers (PEs) included as key components in advanced oral peptide formulations. More than 20 clinical trials have evaluated oral peptide formulations comprising high quantities of SNAC or C₁₀. However, there is still debate concerning the comparative mechanistic properties of SNAC and C₁₀, which are studied in this thesis notably: (i) interactions with payloads, (ii) potential cytotoxicity, (iii) intestinal permeability enhancement capacity and (iv) effects on intestinal mucus. A head to head comparison between SNAC and C₁₀ would assist a decision to opt for one over the other in an oral peptide formulation.Biophysical interactions between SNAC or C₁₀ and a model peptide, exenatide, were similar, dominated by non-covalent weak interactions resulting from supramolecular organization. Cytotoxicity assays combined with High Content Analysis revealed that SNAC and C₁₀ initially fluidized Caco-2 cell apical plasma membranes. Intracellular parameters consequently varied, but with different patterns for each PEs. In transport studies, SNAC differed from C₁₀, as only the latter enhanced FD4 fluxes across Caco-2 monolayers. However, permeation enhancing effects of both PEs was confirmed using rat intestinal tissue mucosae and non-everted gut sacs from jejunum and colon. Although a specific enhancing effect was measured for SNAC and not C₁₀ across isolated rat gastric mucosae stomach. Rheological studies indicate that the gel-like structure of intestinal porcine mucus was preserved when in contact with these PEs. In conclusion, this thesis has revealed the common mechanistic features of surfactants between C₁₀ and SNAC along with subtle differences. C₁₀ had a somewhat higher potency as a PE than SNAC, but efficacy and cytotoxicity profiles were similar
Javot, Lucie. "Etude in vitro et in vivo de deux héparines de bas poids moléculaire microencapsulées de rapports anti-Xa/anti-IIa différents : la nadroparine et la tinzaparine." Thesis, Nancy 1, 2009. http://www.theses.fr/2009NAN10093/document.
Full textMicroparticles of low molecular weight heparin (LMWH) were prepared according to the double emulsion and extraction method using a non biodegradable polycationic polymer (Eudragit® RS) alone or blended according to different ratios with a biodegradable polymer (poly(lactic-co-glycolic) acid). Two LMWH presenting different anti-Xa/anti-IIa ratios were tested: nadroparin (3.6) and tinzaparin (1.8). LMWH microparticles facilitate i) the encapsulation of heparin active long chains (ACL) compared to active short chains (BCL) (anti-Xa/anti-IIa ratio decreased) ii) the in vitro release of short chains (anti-Xa/anti-IIa ratio increased) whereas long chains are held inside the microparticles. Nevertheless, when compared to ACL chains, the relative amount of BCL chains released is dependent (case of nadroparine) or not (case of tinzaparin) on the polymeric composition. This difference can be mainly due to the properties of Eudragit® RS to hold ACL chains of nadroparin. After an oral administration in rabbits of nadroparin microparticles, some formulations exhibited absorption of ACL and BCL chains: in this case, plasmatic anti-Xa/anti-IIa ratios in the same range than those observed following the subcutaneous injection were obtained. Such a result shows the potential of microparticles to be a good substitute of the commercial injectable dosage form, if the oral absorption is confirmed in human. During gastro-intestinal studies by confocal microscopy, the absorption site was not identified. Nevertheless, contrary to in vivo results, permeation studies on a cell cultured epithelium (Caco-2) demonstrated that BCL heparin chains were absorbed whereas ACL chains were not. However, the mechanism responsible of the cell permeation is still to be identified
Schröder, Regina. "Nachweis intrazellulärer Salmonellen in phagozytierenden Zellen nach oraler Infektion von Mäusen." Doctoral thesis, Universitätsbibliothek Leipzig, 2004. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-37599.
Full textThe oral-faecal route is the general way for Salmonella to infect humans and animals. If Salmonella is able to reach the distal ileum and caecum, it can invade the mucosa and cause systemic diseases (septikemia, thyphoid fever). The gut mucosa is the most important barrier, which defense function will be characterised in this work. The PeyerŽs patches are lymphoid tissues and are located in the gut mucosa. They are colocalized with the M-cells in the gut epithelium. In this border region between epithelium and PeyerŽs patches reside a lot of macrophages and dendritic cells. These are antigen presenting cells and they can phagocytize antigen (bacteria), process antigen and present antigen in the lymphoid tissue to naive T-cells to activate them for a specific immune response. We established methods to detect Salmonella antigen and live Salmonellae. With a Salmonella-specific antiserum we could find Salmonella antigen by immunhistological and flow cytometric methods. Live Salmonellae were detected by plating on selective agar plates. Single cells were isolated from PeyerŽs patches and separated in phagocytic cells and B and T cells and analysed by several methods. After in vitro infection of isolated dendritic cells we detected Salmonellae in dendritic cells by electron microscopy. Therefore, Salmonellae are able to infect dendritic cells. 12 hours after oral infection Salmonellae could be detected in phagocytic cells and B and T cells isolated from PeyerŽs patches. The number of infected cells was very low in all cases. Four hours post infection there was about the same frequency of extracellular and intracellular Salmonellae. Salmonella antigen bearing cells were detected by single cell analysis four hours post infection. The analysis showed 0,09 % to 0,61 % cells of PeyerŽs patches or spleen positive for Salmonella antigen. This is only a low number of antigen-presenting cells, but it seems to be enough to induce an effective immune response. The bacterial burden in the PeyerŽs patches was different between mice with and without IL 12. While the bacterial burden of two PeyerŽs patches out of wild-type mice was only seven Salmonellae, two PeyerŽs patches of IL-12 knock out mice carried 28 to 34 Salmonellae. The number of infected cells was the same in wild-type and IL-12 knockout mice. IL-12 is produced during inflammatory responses to pathogens, but it is also available as a membrane-bound pool on macrophages and dendritic cells. It does not influence the phagocytic mechanisms, but has a very important role in inducing bactericidal activity. Therefore, it is possible that IL-12 in wild-type mice allows for a better killing of Salmonellae, while the lack of IL-12 in knockout mice leads to a reduced killing of Salmonellae
Ghorbel-Feki, Halima. "Elaboration et caractérisation de revêtements composites (Apatite-Alumine) sur métal par projection thermique." Thesis, Belfort-Montbéliard, 2016. http://www.theses.fr/2016BELF0304/document.
Full textA considerable amount of research already focused on ceramic biomaterials given their chemical stabilityand high mechanical strength. On the one hand, Fluorapatite (Fa) has recently attracted some attention as analternative to pure hydroxyapatite (Hap) as coating on metallic implants, given its chemical composition which issimilar to the bone mineral and therefore its excellent biocompatibility. On the other hand, alumina is known for itsexcellent bioinertia and is also one of the most widely investigated bioceramics. In this frame, the aim of our workwas to investigate Al2O3-Fap and Hap-Fap composite coatings deposited on stainless steel substrates usingSpraying processes (APS and SHVOF). The mechanical, micro-structural, tribological and biological (in Vitro and inVivo tests) properties of these composite coatings are determined and analysed in order to determine their possibleuse as surigical implant materials
HOANG, THE-CHIEN, and Hoang The Chien. "Research of the Lubricant Properties in Oral Tribology: Establish an In Vitro Friction Testing System for Mimicking Mouth." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/2257vk.
Full textNudo, Paola, Sergio Rizzuti, Guido Danieli, and Andrea Fusiello. "Sviluppo, realizzazione e sperimentazione in vitro di un sistema ottico per il rilievo tridimensionale dell’arcata dentale." Thesis, 2014. http://hdl.handle.net/10955/386.
Full textThis thesis describes the development of a 3D scanning system based on an active vision technique that uses structured light to obtain the impression of the dental arch. Currently to realize a dental prosthesis a special doughy sticky material is adopted to impress the patient’s dental arch. This imprinting may be scanned and then analyzed and modified by C.A.D. software. All this process is sometimes too long, and it may take several weeks to obtain the final prosthesis. At that moment, there are only few works that use techniques to shorten the processing times and produce a three dimensional model of a dental arch, but often they don’t allow to obtain optimal results due to a low resolution and a slow acquisition rate. Among all the studies described on literature, it results that the structured light is a relatively simple technique that allows for a relative high resolution. In the last years, the development of all these systems, has draw the attention to those devices able to obtain a structure of patient’s dental arch as a C.A.D. model. The device developed and described in this thesis is a novel kind of optical scanner that allows for a total elaboration time, for the realization of a three dimensional model, which is lower than those of the systems described in literature. The new optical scanner consists of four digital camera, a multimedia DLP mini-projector and a software to manage the hardware and process the images. It is actuated by step motors on an 2D guide, which is held rigidly fixed to the denture of the patient by a self balanced kinematic chain, that allows for small motions of the patient’s head. This system designed allows the relief and automatic reconstruction through the use of structured light with a particular Gray-Code method. This work is mainly divided in two main parts: 1. The study of the optical system calibration 2. The study of the reconstruction of dental arch The technique used relies on a new version of Gray-Code method; which allows to solve the so called “correspondences problems”. The structured light is produced by a mini-projector, which has to undertake a calibration phase as well as the camera used for image acquisition. The second part of this study is related to the analysis and marge of the acquired images. This problem involves the alignement of the cloud of points obtained from the automatic scanning process by each camera. In this phase, all the errors involved in the whole procedure are also investigated.
Università della Calabria
Zyba, Vitalij. "Wirkung von antiseptischen Mundspüllösungen auf die menschlichen Zellen der Mundschleimhaut - Eine in-vitro-Studie." Doctoral thesis, 2011. http://hdl.handle.net/11858/00-1735-0000-0006-B1F3-B.
Full textDomingues, Nabais Maria Teresa. "High-amylose carboxymethyl starch matrices for oral sustained drug-release : in vitro and in vivo evaluation." Thèse, 2013. http://hdl.handle.net/1866/10943.
Full textUnmodified and modified starches represent a particularly interesting group of biodegradable and abundant excipients. They have been widely used as excipients for various purposes in tablet formulations, such as binders and/or disintegrants. Spray-dried high-amylose sodium carboxymethyl starch (SD HASCA) was recently proposed as an innovating hydrophilic excipient for sustained-release (SR) in solid oral dosage forms. Amorphous high-amylose sodium carboxymethyl starch (HASCA) was first produced by the etherification of high-amylose corn starch with chloroacetate. HASCA was then spray dried to obtain SD HASCA. This new excipient has shown advantageous and effective properties in the production of SR delivery systems. SR matrix tablets prepared from SD HASCA are inexpensive, simple to formulate and easy to produce by direct compression. The main objective of the present research was to continue the development and optimization of matrix tablets using SD HASCA as the retarding excipient in view of their ultimate application as sustained drug-release delivery systems for oral administration. For this purpose, dissolution tests simulating some of the most relevant physiological conditions of the gastrointestinal tract, taking into account the nature of the polymer under investigation, were employed to evaluate the drug-release characteristics and demonstrate the performance of SD HASCA SR formulations. An exploratory clinical study was also carried out to evaluate the SR properties of this new drug delivery system in the gastrointestinal tract. The first article presented in this thesis evaluated the drug-release characteristics and the physical integrity of formulations containing a compressed blend of drug, sodium chloride and SD HASCA in biorelevant media. The influence of different acidic pH values and residence times was investigated. The SR profile from an optimized SD HASCA formulation was not significantly affected by both the acidic pH value and the residence time in the acidic medium. These results suggest a limited influence of intra- and inter-subject variability of gastric pH on the release kinetics from SD HASCA matrices. In addition, the optimized formulation maintained its integrity throughout the duration of the dissolution tests. The exploratory in vivo study demonstrated extended drug absorption after oral administration of SD HASCA matrix tablets and that the matrix tablets did not disintegrate while passing through the stomach and resisted hydrolysis by α-amylase in the intestine. The second article reports the development of once-daily and twice-daily SD HASCA tablets containing tramadol hydrochloride (100 mg and 200 mg). These SR formulations presented high crushing strengths without requiring the addition of binders, which facilitates tablet processing and handling. The compression force (CF) applied to produce the tablets did not significantly affect the drug-release profiles. The total release time from SD HASCA tablets increased significantly in function of the tablet weight and can be used to modulate the total release time from theses formulations. When exposed to a pH gradient and to a 40% ethanol medium, a very rigid gel formed progressively on the surface of the tablets providing controlled drug-release properties. These properties indicated that SD HASCA is a robust excipient for oral, sustained drug-release, likely to minimize the possibility of dose dumping and consequent adverse effects, even when co-administered with high doses of alcohol. The third article investigated the effect of α-amylase on drug-release from previously developed SD HASCA tablets containing acetaminophen and tramadol hydrochloride (Acetaminophen SR and Tramadol SR). Mathematical modeling showed that an increase in α-amylase concentration resulted in an increase of polymer erosion over drug diffusion as the main mechanism controlling drug-release, for both formulations and both residence times in acidic medium. However, even if the mechanism of release was affected, α-amylase concentrations ranging from 0 IU/L to 20000 IU/L did not significantly affect the drug-release profiles from SD HASCA SR tablets, regardless of the residence time in acidic medium, the drug used, the polymer content and the different composition of each formulation. The work presented in this thesis clearly demonstrates the value of SD HASCA as an efficient SR excipient.
Charette, Tania. "Évaluation des facteurs de modulation de l’exposition du consommateur de poissons et de mammifères marins au méthylmercure : utilisation d’approches in vitro, in vivo et probabiliste." Thesis, 2020. http://hdl.handle.net/1866/25608.
Full textFish flesh and marine mammals is an important source of proteins and nutrients, such as selenium (Se), vitamin E and long chain polyunsaturated fatty acids. However, flesh of those animals may bioaccumulate the organic form of mercury (Hg), methylmercury (MeHg). This contaminant has been the subject of various epidemiological studies, namely because of its neurotoxicity through in utero exposure, closely related to highly MeHg contaminated fish consumption. In response to this toxicological risk, health authorities have set fish consumption guidelines in order to protect the population. Still, those guidelines present limits that are related to premises and omissions of the determinist equation used by Health Canada in order to assess the exposure to MeHg: (1) it considers that 100% of Hg in fish flesh is MeHg, (2) it supposes that MeHg is homogeneously distributed within fish flesh, (3) it takes for granted that 100% of MeHg will be absorbed by the consumer and (4) by using a determinist approach, they omit the potential uncertainty and intra-population variability in the data. The aim of this thesis was to address these limits, in order to better understand the exposure of MeHg for consumers. We first assessed the distribution of MeHg, Se (MeHg antagonist) and arsenic (As, Se antagonist) within fish musculature, as a function of its biomolecule composition (proteins and lipids). Our results demonstrated that the concomitant presence of white and red muscle induces a large gradient of protein and lipid within the muscular apparatus of the same individual. This in turn causes on average a variation by 2.2-fold regarding MeHg, Se and As bioaccumulation, which are distributed according to their biochemical affinity. Those results confirmed that MeHg can distribute heterogeneously within fish muscle, which could lead to an under- or overestimation of MeHg exposure for consumers, as function of the part of the fish consumed. Subsequently, we focused on the hypothesis stipulating that 100% of MeHg is absorbed by the consumer. A method used to assess the fraction of MeHg that would be available to be further absorbed by the gut wall consists of measuring the bioaccessibility, i.e. the soluble fraction of MeHg, using an in vitro digestion model. Several studies that assessed the bioaccessibility of cooked fish flesh observed a decreased of MeHg solubility, that would potentially diminish its intestinal absorption. However, those results have not been yet validated in vivo. To that end, we conducted an in vivo experience using the pig model and, in parallel, we used an in vitro digestion model. According to the pig’s blood profile, the oral bioavailability of MeHg from cooked tuna flesh is not less bioavailable than the MeHg from the raw tuna. Contrasting results have been found with the in vitro model, with a decrease of MeHg bioaccessibility observed when fish flesh is cooked. Our results demonstrated that in vitro digestion models are not optimized to be directly used in MeHg exposure calculus as recently proposed in the literature. Finally, we tested the impact of adding variables to the deterministic equation currently used by Health Canada to assess consumer exposure to MeHg, by conducting a probabilistic risk assessment. We considered (1) the proportion of Hg that is MeHg, (2) the MeHg bioaccessibility and (3) the increased of MeHg level after the cooking of fish flesh due to moisture loss. Our results showed that each individual variable significantly increases or decreases the calculated exposure. Thereby, it highlights the sensitivity of the equation used to assess the exposure of MeHg. It strongly suggests that more research is needed to improve Hg exposure calculation to avoid underestimating the potential health risks of MeHg exposure. This thesis presents important results regarding the exposure of MeHg through fish and marine mammals’ consumption. This thesis shows that the recommendations are difficult to generalize since the flesh of fish and mammals has different properties, depending on the animal species considered. The conclusions of this thesis demonstrate that the premises and omissions of the deterministic equation used by Health Canada in the assessment of exposure to Hg should be better investigated by the scientific sphere, especially in the case of marine mammals.
Schröder, Regina. "Nachweis intrazellulärer Salmonellen in phagozytierenden Zellen nach oraler Infektion von Mäusen." Doctoral thesis, 2003. https://ul.qucosa.de/id/qucosa%3A10983.
Full textThe oral-faecal route is the general way for Salmonella to infect humans and animals. If Salmonella is able to reach the distal ileum and caecum, it can invade the mucosa and cause systemic diseases (septikemia, thyphoid fever). The gut mucosa is the most important barrier, which defense function will be characterised in this work. The PeyerŽs patches are lymphoid tissues and are located in the gut mucosa. They are colocalized with the M-cells in the gut epithelium. In this border region between epithelium and PeyerŽs patches reside a lot of macrophages and dendritic cells. These are antigen presenting cells and they can phagocytize antigen (bacteria), process antigen and present antigen in the lymphoid tissue to naive T-cells to activate them for a specific immune response. We established methods to detect Salmonella antigen and live Salmonellae. With a Salmonella-specific antiserum we could find Salmonella antigen by immunhistological and flow cytometric methods. Live Salmonellae were detected by plating on selective agar plates. Single cells were isolated from PeyerŽs patches and separated in phagocytic cells and B and T cells and analysed by several methods. After in vitro infection of isolated dendritic cells we detected Salmonellae in dendritic cells by electron microscopy. Therefore, Salmonellae are able to infect dendritic cells. 12 hours after oral infection Salmonellae could be detected in phagocytic cells and B and T cells isolated from PeyerŽs patches. The number of infected cells was very low in all cases. Four hours post infection there was about the same frequency of extracellular and intracellular Salmonellae. Salmonella antigen bearing cells were detected by single cell analysis four hours post infection. The analysis showed 0,09 % to 0,61 % cells of PeyerŽs patches or spleen positive for Salmonella antigen. This is only a low number of antigen-presenting cells, but it seems to be enough to induce an effective immune response. The bacterial burden in the PeyerŽs patches was different between mice with and without IL 12. While the bacterial burden of two PeyerŽs patches out of wild-type mice was only seven Salmonellae, two PeyerŽs patches of IL-12 knock out mice carried 28 to 34 Salmonellae. The number of infected cells was the same in wild-type and IL-12 knockout mice. IL-12 is produced during inflammatory responses to pathogens, but it is also available as a membrane-bound pool on macrophages and dendritic cells. It does not influence the phagocytic mechanisms, but has a very important role in inducing bactericidal activity. Therefore, it is possible that IL-12 in wild-type mice allows for a better killing of Salmonellae, while the lack of IL-12 in knockout mice leads to a reduced killing of Salmonellae.
Melkoumov, Alexandre. "Nanoformulations de nystatine pour une efficacité antifongique améliorée." Thèse, 2013. http://hdl.handle.net/1866/10408.
Full textHypothesis : Nanomilling of a nystatin suspension will increase its antifungal efficacy in vitro and in vivo. Methods: A nystatin nanosuspension was obtained using wet bead milling. It was characterized for its particle size distribution and for its drug content. In vitro activity was evaluted against C. albicans strains SC5314 and LAM-1 at concentrations of 12.5 μg/mL up to 5000 μg/mL. The in vivo efficacy was evaluated using a murine model of oropharyngeal candidiasis. Results: Median particle size of the nystatin nanosuspension was reduced from 6577 nm to 137 nm. HPLC analysis demonstrated a content assay of 98.7 ± 0.8%. In vitro activity of the nanosuspension was superior to the suspension’s at concentrations ranging from 100 μg/mL to 5000 μg/mL. Oral fungal burdens were inferor in the nanosuspension group compared to the suspension and saline groups. Mice survival was also superior in the nanosuspension group.