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Journal articles on the topic 'Transzonal projections'

Author: Grafiati

Published: 25 May 2024

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1

Perecin, Felipe. "412 Germ and somatic cell interactions during oocyte development and maturation." Journal of Animal Science 98, Supplement_4 (2020): 189. http://dx.doi.org/10.1093/jas/skaa278.349.

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Abstract Ovarian follicle development and oocyte competence acquisition is dependent upon continuous interactions between the somatic cells and the oocyte. Interactions between these cell types include bidirectional paracrine signaling and the exchange of small molecules, such and amino acids and cyclic nucleotides, through gap junctions located at the end of transzonal projections (TZPs). In the last decade, additional mechanisms of cell-to-cell interactions within the ovarian follicle were described. These mechanisms include the movement of small extracellular vesicles (EVs) within the folli

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2

Stoecklein, K. S., M. S. Ortega, L. Spate, C. N. Murphy, and R. S. Prather. "188 Improvement of bovine oocyte maturation invitro through cytokine supplementation." Reproduction, Fertility and Development 32, no. 2 (2020): 222. http://dx.doi.org/10.1071/rdv32n2ab188.

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Oocyte competence is one of the key factors determining the proportion of embryos that develop to the blastocyst stage. There is vast evidence that IVM oocytes exhibit less developmental potential than their invivo counterparts. Here, we tested whether supplementation of three cytokines [FGF2 (40 ngmL−1), LIF (20ngmL−1), and IGF1 (20 ngmL−1), termed FLI] improved oocyte maturation, and as a consequence, preimplantation development of bovine embryos invitro. In the first experiment, cumulus-oocyte complexes (COCs) were collected from abattoir-derived ovaries and placed in maturation medium,±FLI

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Feng, Xiaoyi, Chongyang Li, Hang Zhang, et al. "Heat-Stress Impacts on Developing Bovine Oocytes: Unraveling Epigenetic Changes, Oxidative Stress, and Developmental Resilience." International Journal of Molecular Sciences 25, no. 9 (2024): 4808. http://dx.doi.org/10.3390/ijms25094808.

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Extreme temperature during summer may lead to heat stress in cattle and compromise their productivity. It also poses detrimental impacts on the developmental capacity of bovine budding oocytes, which halt their fertility. To mitigate the adverse effects of heat stress, it is necessary to investigate the mechanisms through which it affects the developmental capacity of oocytes. The primary goal of this study was to investigate the impact of heat stress on the epigenetic modifications in bovine oocytes and embryos, as well as on oocyte developmental capacity, reactive oxygen species, mitochondri

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Chen, Mingyue, Chengyong He, Kongyang Zhu, et al. "Resveratrol ameliorates polycystic ovary syndrome via transzonal projections within oocyte-granulosa cell communication." Theranostics 12, no. 2 (2022): 782–95. http://dx.doi.org/10.7150/thno.67167.

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Clarke, Hugh J. "History, origin, and function of transzonal projections: the bridges of communication between the oocyte and its environment." Animal Reproduction 15, no. 3 (2018): 215–23. http://dx.doi.org/10.21451/1984-3143-ar2018-0061.

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Lee, Seunghoon, Yuuki Hiradate, Yumi Hoshino, Yeoung-gyu Ko, Kentaro Tanemura, and Eimei Sato. "Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation." Zygote 24, no. 3 (2015): 364–70. http://dx.doi.org/10.1017/s0967199415000271.

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SummaryMany studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus–oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oo

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7

Robert, Claude. "Nurturing the egg: the essential connection between cumulus cells and the oocyte." Reproduction, Fertility and Development 34, no. 2 (2022): 149. http://dx.doi.org/10.1071/rd21282.

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The determinants of oocyte quality remain uncertain. Under suitable conditions, which have yet to be defined, the gamete grows and acquires the competence to resume meiosis, be fertilised and undergo embryonic development at least beyond genome activation, after which the blastomere is autonomous enough to adapt to the specificity of its environment. This review describes the central role played by the oocyte in reproductive success and how communication between cumulus cells and the oocyte are essential to proper oogenesis and the quality of the resulting gamete. While most attempts to improv

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Crozet, Flora, Gaëlle Letort, Rose Bulteau, et al. "Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes." Life Science Alliance 6, no. 6 (2023): e202301963. http://dx.doi.org/10.26508/lsa.202301963.

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The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by the somatic cells to the oocyte membrane. To investigate the contribution of TZPs to oocyte quality, we impaired their structure by generating a full knockout mouse of the TZP structural component myosin-X (MYO10). Using spinning disk and super-resolution microscopy combined with a machine-learning approach to phenotype oocyte morphology, we show that the lack ofM

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9

Xu, Rui, Menghao Pan, Lu Yin, et al. "C-Type Natriuretic Peptide Pre-Treatment Improves Maturation Rate of Goat Oocytes by Maintaining Transzonal Projections, Spindle Morphology, and Mitochondrial Function." Animals 13, no. 24 (2023): 3880. http://dx.doi.org/10.3390/ani13243880.

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C-type natriuretic peptide (CNP) is a peptide molecule naturally found in follicles and can be used to extend meiotic resumption and enhance the potential for oocytes to develop. However, the mechanism by which CNP improves goat oocyte quality remains unclear. In this study, cumulus–oocyte complexes (COCs) from goats were pre-treated with CNP prior to IVM, and the results showed that pre-treatment with CNP enhanced goat oocyte maturation. First, we discovered that CNP maintained communication between cumulus cells and oocytes by regulating the transzonal projections (TZPs). We then found that

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10

Nagyová, Eva, Lucie Němcová, and Antonella Camaioni. "Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation." International Journal of Molecular Sciences 23, no. 1 (2021): 54. http://dx.doi.org/10.3390/ijms23010054.

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The extracellular matrix (ECM) is an essential structure with biological activities. It has been shown that the ECM influences gene expression via cytoskeletal components and the gene expression is dependent upon cell interactions with molecules and hormones. The development of ovarian follicles is a hormone dependent process. The surge in the luteinizing hormone triggers ovulatory changes in oocyte microenvironment. In this review, we discuss how proteolytic cleavage affects formation of cumulus ECM following hormonal stimulation; in particular, how the specific proteasome inhibitor MG132 aff

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Appeltant, Ruth, Tamás Somfai, Elisa C. S. Santos, Thanh Quang Dang-Nguyen, Takashi Nagai, and Kazuhiro Kikuchi. "Effects of vitrification of cumulus-enclosed porcine oocytes at the germinal vesicle stage on cumulus expansion, nuclear progression and cytoplasmic maturation." Reproduction, Fertility and Development 29, no. 12 (2017): 2419. http://dx.doi.org/10.1071/rd16386.

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Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus–oocyte complexes (COCs) and control COCs (no cryoprotectant treatment and no cooling), as well as a toxicity control (no cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol and 17.5% (v/v) propylene glycol. Vitrification at the GV stage caused prematu

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Wang, Yan, Hualin Huang, Minghua Zeng, et al. "Mutation of rat Zp2 causes ROS-mediated oocyte apoptosis." Reproduction 160, no. 3 (2020): 353–65. http://dx.doi.org/10.1530/rep-20-0037.

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In this study, we investigated a gene-edited (Zp2MT/MT) rat model of infertility caused by the failure to express the zona pellucida glycoprotein 2 (ZP2) due to the significant reduction of mRNA amount. We examined the defects in the zona pellucida (ZP) caused by ZP2 nullification and the influence of these defects on aspects of oocyte development, including apoptosis and fertilization ability. To investigate the cause of the influence to the oocytes’ development, we evaluated the morphology of follicular transzonal projections (TZPs), known as ‘bridges’, which mediate the bidirectional signal

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Caballero, Julieta, Isabelle Gilbert, Eric Fournier, et al. "Exploring the function of long non-coding RNA in the development of bovine early embryos." Reproduction, Fertility and Development 27, no. 1 (2015): 40. http://dx.doi.org/10.1071/rd14338.

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Now recognised as part of the cellular transcriptome, the function of long non-coding (lnc) RNA remains unclear. Previously, we found that some lncRNA molecules in bovine embryos are highly responsive to culture conditions. In view of a recent demonstration that lncRNA may play a role in regulating important functions, such as maintenance of pluripotency, modification of epigenetic marks and activation of transcription, we sought evidence of its involvement in embryogenesis. Among the numerous catalogued lncRNA molecules found in oocytes and early embryos of cattle, three candidates chosen for

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14

Wang, Yan, Chao Lv, Hua-Lin Huang, et al. "Influence of mouse defective zona pellucida in folliculogenesis on apoptosis of granulosa cells and developmental competence of oocytes†." Biology of Reproduction 101, no. 2 (2019): 457–65. http://dx.doi.org/10.1093/biolre/ioz093.

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Abstract Zona pellucida (ZP), which enwraps the oocyte during folliculogenesis, initially forms in the primary follicle and plays an important role in female fertility. Here, we investigated a mouse strain (“mutant mice” for short) carrying two types of ZP defects in folliculogenesis, i.e., ZP thinned (but intact) and ZP cracked, caused by targeted mutation in the Zp1 gene. Using this mutant mouse strain and wild-type mouse as control, we studied the effects of the ZP defects on the development of oocytes and granulosa cells during folliculogenesis. For each ZP defect, we examined the morpholo

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15

Stoecklein, Katy S., M. Sofia Ortega, Lee D. Spate, Clifton N. Murphy, and Randall S. Prather. "Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1." PLOS ONE 16, no. 2 (2021): e0243727. http://dx.doi.org/10.1371/journal.pone.0243727.

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In vitro embryo production systems are limited by their inability to consistently produce embryos with the competency to develop to the blastocyst stage, survive cryopreservation, and establish a pregnancy. Previous work identified a combination of three cytokines [fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1)], called FLI, that we hypothesize improve preimplantation development of bovine embryos in vitro. To test this hypothesis, FLI was supplemented into oocyte maturation or embryo culture medium. Embryos were produced in vitro u

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Grosbois, J., M. Vermeersch, M. Devos, H. J. Clarke, and I. Demeestere. "Ultrastructure and intercellular contact-mediated communication in cultured human early stage follicles exposed to mTORC1 inhibitor." Molecular Human Reproduction 25, no. 11 (2019): 706–16. http://dx.doi.org/10.1093/molehr/gaz053.

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Abstract The reproductive lifespan of a woman is determined by the gradual recruitment of quiescent follicles into the growing pool. In humans, ovarian tissue removal from its in vivo environment induces spontaneous activation of resting follicles. Similarly, pharmacological activation of the PI3K/Akt pathway leads to accelerated follicle recruitment, but has been associated with follicular damage. Recent findings demonstrate that everolimus (EVE), an mTORC1 inhibitor, limits primordial follicle activation. However, its potential benefit regarding growing follicle integrity remains unexplored.

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17

Zhang, Peipei, Baigao Yang, Xi Xu, et al. "Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos." Antioxidants 12, no. 4 (2023): 897. http://dx.doi.org/10.3390/antiox12040897.

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Oocyte maturation is a critical step in the completion of female gametogenesis in the ovary; thus, for subsequent fertilization and embryogenesis. Vitrification of embryo also has been shown to be closely associated with oocyte maturation. To improve the quality and developmental potential of bovine oocytes derived from in vitro maturation (IVM), Pre-IVM with C-type natriuretic peptide (CNP), melatonin (MT) and in combination, IGF1, FGF2, LIF (FLI) were supplemented in the IVM medium. In this current study, we cultured bovine oocytes in Pre-IVM with CNP for 6 h before transferring them to the

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18

Martínez-Moro, Á., I. Lamas-Toranzo, and P. Bermejo-Álvarez. "127 Metabolomics analysis of human cumulus cells from oocytes exhibiting different developmental competence." Reproduction, Fertility and Development 33, no. 2 (2021): 172. http://dx.doi.org/10.1071/rdv33n2ab127.

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Cumulus cells play fundamental metabolic roles during folliculogenesis, being closely connected to the oocyte through transzonal projections. These oocyte-supporting cells are removed and discarded before intracytoplasmic sperm injection (ICSI), thereby constituting an interesting biological material on which to perform molecular analyses aimed to predict oocyte developmental potential. The objective of this study was to determine the possible differences in the amount of biochemical compounds in human cumulus cells from oocytes exhibiting different developmental competence: (1) oocytes not de

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Martínez-Moro, Á., I. Lamas-Toranzo, and P. Bermejo-Álvarez. "127 Metabolomics analysis of human cumulus cells from oocytes exhibiting different developmental competence." Reproduction, Fertility and Development 33, no. 2 (2021): 172. http://dx.doi.org/10.1071/rdv33n2ab127.

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Cumulus cells play fundamental metabolic roles during folliculogenesis, being closely connected to the oocyte through transzonal projections. These oocyte-supporting cells are removed and discarded before intracytoplasmic sperm injection (ICSI), thereby constituting an interesting biological material on which to perform molecular analyses aimed to predict oocyte developmental potential. The objective of this study was to determine the possible differences in the amount of biochemical compounds in human cumulus cells from oocytes exhibiting different developmental competence: (1) oocytes not de

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20

Keim, J., Y. Liu, and I. Polejaeva. "177 Increasing in vitro embryonic development through improved oocyte maturation in cattle oocytes." Reproduction, Fertility and Development 31, no. 1 (2019): 213. http://dx.doi.org/10.1071/rdv31n1ab177.

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In vitro maturation (IVM) is an important process in the in vitro production of embryos. It has been recently shown that 3 cytokines: fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1) have increased the efficiency of IVM, blastocyst production, and in vivo development in pig (Yuan et al. 2017 Proc. Natl. Acad. Sci. USA 114, E5796-E5804). In vitro maturation in medium supplemented with cytokines doubled the blastocyst rate and quadrupled the litter size when transferred. It was observed that the addition of cytokines to IVM medium had a

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Buratini, Jose, Thaisy Tino Dellaqua, Mariabeatrice Dal Canto, et al. "The putative roles of FSH and AMH in the regulation of oocyte developmental competence: from fertility prognosis to mechanisms underlying age-related subfertility." Human Reproduction Update 28, no. 2 (2021): 232–54. http://dx.doi.org/10.1093/humupd/dmab044.

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Abstract BACKGROUND Fertility loss during female ageing is associated with increasing basal FSH and decreasing anti-Müllerian hormone (AMH) concentrations, together with compromised oocyte quality, presumably due to increased oxidative stress (OS) and DNA damage, as well as reduced metabolic and meiotic competences. Basal FSH and AMH circulatory concentrations have been broadly utilized as IVF success predictors, regardless of fluctuations in prognostic accuracy; basal FSH and AMH perform better in pre-advanced maternal age (AMA: &gt;35 years) and AMA patients, respectively. The relationsh

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Yang, Sha, Yuze Yang, Haisheng Hao, et al. "Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts." Genes 13, no. 5 (2022): 805. http://dx.doi.org/10.3390/genes13050805.

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The quality and developmental capacity of oocytes derived from in vitro maturation (IVM) remain unsatisfactory, which greatly impairs the efficiency and application of embryo technologies. The present experiment was designed to investigate the effect of the supplementation of EGF, IGF-1, and Cx37 in an IVM medium on the maturation quality and development ability of bovine oocytes. The cytoplasmic maturation events of oocytes and the quality of in vitro fertilization (IVF) blastocysts were examined to investigate the relative mechanisms. Our results showed that the nuclear maturation and blasto

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Albertini, DF, CM Combelles, E. Benecchi, and MJ Carabatsos. "Cellular basis for paracrine regulation of ovarian follicle development." Reproduction, May 1, 2001, 647–53. http://dx.doi.org/10.1530/rep.0.1210647.

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Paracrine factors secreted by oocytes and somatic cells regulate many important aspects of early ovarian follicle development in mammals. From activation of dormant primordial follicles to selection of secondary follicles, locally acting factors have been identified that appear to exert important effects on the growth and differentiation of oocytes and granulosa cells. This article summarizes evidence to support a model for bi-directional paracrine communication that is based on developmental regulation of the delivery and reception of paracrine factors at the oocyte-granulosa cell interface.

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Nenonene, Elolo Karen, Mallorie Trottier-Lavoie, Mathilde Marchais, et al. "Roles of the cumulus-oocyte transzonal network and the Fragile X protein family in oocyte competence." Reproduction, November 2022. http://dx.doi.org/10.1530/rep-22-0165.

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The determinants of oocyte developmental competence have puzzled scientists for decades. It is known that follicular conditions can nurture the production of a high-quality oocyte, but the underlying mechanisms remain unknown. Somatic cumulus cells most proximal to the oocyte are known to have cellular extensions that reach across the zona pellucida and contact with the oocyte plasma membrane. Herein, it was found that transzonal projections (TZPs) network quality is associated with developmental competence. Knowing that ribonucleo-particles are abundant within TZPs, the distribution of RNA bi

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Clarke, Hugh J. "Transzonal projections: Essential structures mediating intercellular communication in the mammalian ovarian follicle." Molecular Reproduction and Development, September 16, 2022. http://dx.doi.org/10.1002/mrd.23645.

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FUSHII, Mihoko, Rie YAMADA, Jibak LEE, and Takashi MIYANO. "Reestablishment of transzonal projections and growth of bovine oocytes in vitro." Journal of Reproduction and Development, 2021. http://dx.doi.org/10.1262/jrd.2021-036.

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27

Baena, Valentina, and Mark Terasaki. "Three-dimensional organization of transzonal projections and other cytoplasmic extensions in the mouse ovarian follicle." Scientific Reports 9, no. 1 (2019). http://dx.doi.org/10.1038/s41598-018-37766-2.

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Granados-Aparici, Sofia, Alexander Volodarsky-Perel, Qin Yang, et al. "MYO10 promotes transzonal projection (TZP)-dependent germ line-somatic contact during mammalian folliculogenesis." Biology of Reproduction, April 26, 2022. http://dx.doi.org/10.1093/biolre/ioac078.

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Abstract Granulosa cells of growing ovarian follicles elaborate filopodia-like structures termed transzonal projections (TZPs) that supply the enclosed oocyte with factors essential for its development. Little is known, however, of the mechanisms underlying the generation of TZPs. We show in mouse and human that filopodia, defined by an actin backbone, emerge from granulosa cells in early-stage primary follicles and that actin-rich TZPs become detectable as soon as a space corresponding to the zona pellucida appears. mRNA encoding Myosin10 (MYO10), a motor protein that accumulates at the base

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Granados-Aparici, Sofia, Alexander Volodarsky-Perel, Qin Yang, et al. "MYO10 promotes transzonal projection (TZP)-dependent germ line-somatic contact during mammalian folliculogenesis." Biology of Reproduction, April 26, 2022. http://dx.doi.org/10.1093/biolre/ioac078.

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Abstract Granulosa cells of growing ovarian follicles elaborate filopodia-like structures termed transzonal projections (TZPs) that supply the enclosed oocyte with factors essential for its development. Little is known, however, of the mechanisms underlying the generation of TZPs. We show in mouse and human that filopodia, defined by an actin backbone, emerge from granulosa cells in early-stage primary follicles and that actin-rich TZPs become detectable as soon as a space corresponding to the zona pellucida appears. mRNA encoding Myosin10 (MYO10), a motor protein that accumulates at the base

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Herta, Anamaria-Cristina, Nazli Akin, Katy Billooye, et al. "Reversing complete mechanical transzonal projections disruption during mouse in vitro follicle culture with unaltered oocyte competence†." Biology of Reproduction, March 11, 2021. http://dx.doi.org/10.1093/biolre/ioab045.

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Abstract In vitro oocyte growth is widely studied as an alternative fertility preservation approach. Several animal models are used to generate extensive information on this complex process regulated by the constant and dynamic interaction between the oocyte and its somatic compartment throughout follicle growth and maturation. A two-dimensional attachment mouse secondary follicle culture system was used to assess the oocyte’s capacity to overcome disconnection from its somatic companions at different developmental stages for final competence acquisition. To test this, complete mechanical denu

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Bus, Anniek, Katarzyna Szymanska, Isabel Pintelon, Jo L. M. R. Leroy, Luc Leybaert, and Peter E. J. Bols. "Preservation of connexin 43 and transzonal projections in isolated bovine pre-antral follicles before and following vitrification." Journal of Assisted Reproduction and Genetics, November 6, 2020. http://dx.doi.org/10.1007/s10815-020-01993-2.

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Bus, Anniek, Katarzyna Szymanska, Isabel Pintelon, Jo L. M. R. Leroy, Luc Leybaert, and Peter E. J. Bols. "Correction to: Preservation of connexin 43 and transzonal projections in isolated bovine pre-antral follicles before and following vitrification." Journal of Assisted Reproduction and Genetics, December 17, 2020. http://dx.doi.org/10.1007/s10815-020-02040-w.

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del Collado, Maite, Juliano Coelho da Silveira, Juliano Rodrigues Sangalli, et al. "Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes." Scientific Reports 7, no. 1 (2017). http://dx.doi.org/10.1038/s41598-017-02467-9.

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Yin, Chao, Jie Liu, Zhanglin Chang, Bin He, Yang Yang, and Ruqian Zhao. "Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projections." Journal of Animal Science and Biotechnology 11, no. 1 (2020). http://dx.doi.org/10.1186/s40104-020-00477-8.

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Abstract Background Transzonal projections (TZPs) constitute a structural basis for the communication between the oocyte and its surrounding cumulus cells (CCs), which play critical roles in promoting the oocyte maturation. Previously we found that heat stress (HS) causes loss of TZPs in porcine cumulus-oocyte complexes (COCs) with decreased density of filamentous actin (F-actin). However, the time-course responses of F-actin and its monomeric actins (β-actin and γ-actin) during the in vitro maturation of oocytes remain unclear. Results In this study, excised porcine ovaries were exposed to HS

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Jankovičová, Jana, Petra Sečová, Ľubica Horovská, et al. "Distribution of tetraspanins in bovine ovarian tissue and fresh/vitrified oocytes." Histochemistry and Cell Biology, October 15, 2022. http://dx.doi.org/10.1007/s00418-022-02155-4.

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AbstractTetraspanin proteins are mostly known as organizers of molecular complexes on cell membranes, widely expressed on the surface of most nucleated cells. Although tetraspanins participate in many physiological processes of mammals, including reproduction, their relevance to the processes of folliculogenesis and oogenesis has not yet been fully elucidated. We bring new information regarding the distribution of tetraspanins CD9, CD81, CD151, CD82, and CD63 at different stages of follicular development in cattle. The found distribution of tetraspanin CD9, CD63, and integrin alpha V in simila

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Cheng, Kaixin, Xie’an Feng, Chen Yang, et al. "Simulated microgravity reduces quality of ovarian follicles and oocytes by disrupting communications of follicle cells." npj Microgravity 9, no. 1 (2023). http://dx.doi.org/10.1038/s41526-023-00248-5.

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AbstractOvarian follicles are the fundamental structures that support oocyte development, and communications between oocytes and follicle somatic cells are crucial for oogenesis. However, it is unknown that whether exposure to microgravity influences cellular communications and ovarian follicle development, which might be harmful for female fertility. By 3D culturing of ovarian follicles under simulated microgravity (SMG) conditions in a rotating cell culture system, we found that SMG treatment did not affect the survival or general growth of follicles but decreased the quality of cultured fol

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Converse, Aubrey, Zhenghui Liu, Jai C. Patel, et al. "Hypoglycosylated FSH enhances oocyte quality via increased cell-to-cell interaction during mouse follicle development." Development, October 23, 2023. http://dx.doi.org/10.1242/dev.202170.

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Macroheterogeneity in follicle-stimulating hormone (FSH) β-subunit N-glycosylation results in distinct FSH glycoforms. Hypoglycosylated FSH21 is the abundant and more bioactive form in pituitaries of young women, whereas fully glycosylated FSH24 is less bioactive and increases with age. To investigate if the shift in FSH glycoform abundance contributes to the age-dependent decline in oocyte quality, the direct effects of FSH glycoforms on folliculogenesis and oocyte quality were determined using an encapsulated in vitro follicle growth system. Long-term culture (10-12 days) with FSH21 (10 ng/m

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Ang, Li, Guo Xingping, Cao Haixia, Wang Zhulin, and Wang Huaixiu. "Assessment of cGMP level in medium during in vitro growth period of murine preantral follicles with and without supplementation of C-type natriuretic peptide." Zygote, June 22, 2021, 1–5. http://dx.doi.org/10.1017/s0967199421000393.

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Summary To enhance the developmental competency of murine ovarian follicles cultured in vitro, C-type natriuretic peptide (CNP) was supplemented in the culture system. Although the mechanism is not fully elucidated, it was reported that the effect of CNP supplementation was mediated by increased cyclic guanosine monophosphate (cGMP). In the present study, cGMP levels in media for murine preantral follicle culture were compared both between a control group without CNP supplementation and an experimental group with CNP supplementation and between days in each group. In addition, follicle growth

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Catandi, G. D., D. R. Bresnahan, S. O. Peters, et al. "Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points." Frontiers in Cell and Developmental Biology 11 (September 25, 2023). http://dx.doi.org/10.3389/fcell.2023.1239154.

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Introduction: Oocyte quality and fertility decline with advanced maternal age. During maturation within the ovarian follicle, the oocyte relies on the associated somatic cells, specifically cumulus and granulosa cells, to acquire essential components for developmental capacity.Methods: A nontargeted metabolomics approach was used to investigate the effects of mare age on different cell types within the dominant, follicular-phase follicle at three time points during maturation. Metabolomic analyses from single oocytes and associated cumulus and granulosa cells allowed correlations of metabolite

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Xie, Jun, Xiao Xu, and Suying Liu. "Intercellular communication in the cumulus–oocyte complex during folliculogenesis: A review." Frontiers in Cell and Developmental Biology 11 (January 19, 2023). http://dx.doi.org/10.3389/fcell.2023.1087612.

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During folliculogenesis, the oocyte and surrounding cumulus cells form an ensemble called the cumulus-oocyte complex (COC). Due to their interdependence, research on the COC has been a hot issue in the past few decades. A growing body of literature has revealed that intercellular communication is critical in determining oocyte quality and ovulation. This review provides an update on the current knowledge of COC intercellular communication, morphology, and functions. Transzonal projections (TZPs) and gap junctions are the most described structures of the COC. They provide basic metabolic and nu

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Marchais, Mathilde, Isabelle Gilbert, Alexandre Bastien, Angus Macaulay, and Claude Robert. "Mammalian cumulus-oocyte complex communication: a dialog through long and short distance messaging." Journal of Assisted Reproduction and Genetics, May 2, 2022. http://dx.doi.org/10.1007/s10815-022-02438-8.

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Abstract Communications are crucial to ovarian follicle development and to ovulation, and while both folliculogenesis and oogenesis are distinct processes, they share highly interdependent signaling pathways. Signals from distant organs such as the brain must be processed and compartments within the follicle have to be synchronized. The hypothalamic–pituitary–gonadal (HPG) axis relies on long-distance signalling analogous to wireless communication by which data is disseminated in the environment and cells equipped with the appropriate receptors receive and interpret the messages. In contrast,

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Barrozo, Laryssa G., Bianca R. Silva, Laís R. F. M. Paulino, et al. "N-Acetyl cysteine reduces the levels of reactive oxygen species and improves in vitro maturation of oocytes from medium-sized bovine antral follicles." Zygote, September 23, 2022, 1–9. http://dx.doi.org/10.1017/s0967199422000429.

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Summary This study aims to evaluate the effects of N-acetylcysteine (NAC) on bovine oocyte maturation, mitochondrial activity and transzonal projections (TZP), as well as on the levels of reactive oxygen species (ROS) and messenger RNA (mRNA) for catalase (CAT) superoxide dismutase (SOD), periredoxin-6 (Prdx6), glutathione peroxidase (GPx), growth and differentiation factor-9 (GDF9), histone H1Foo, cyclin B1 (CCNB1) and c-Mos. Bovine cumulus–oocyte complexes (COC) of medium-sized antral follicles (3.0–6.0 mm) were prematured in TCM-199 for 8 h at 38.5°C in 5% CO2. After prematuration in the pr

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Dubuc, Karine, Mathilde Marchais, Isabelle Gilbert, et al. "Epitranscriptome marks detection and localization of RNA modifying proteins in mammalian ovarian follicles." Journal of Ovarian Research 16, no. 1 (2023). http://dx.doi.org/10.1186/s13048-023-01172-8.

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Abstract Background Most of the resources that support the early development of the embryo are stored in the oocyte. Clearing of maternal resources and activation of the embryonic genome to produce its own mRNA transcripts marks the maternal-to-embryo transition. Dependence on stored mRNA can last from a few hours to several days, depending on animal species. The mechanisms regulating stabilization and recruitment of stored maternal transcripts have not yet been described in full detail but are known to involve reversible polyadenylation and modulation of 3’UTR-mediated elements. RNA epigeneti

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Martínez-Moro, Álvaro, Ismael Lamas-Toranzo, Leopoldo González-Brusi, et al. "mtDNA content in cumulus cells does not predict development to blastocyst or implantation." Human Reproduction Open, July 6, 2022. http://dx.doi.org/10.1093/hropen/hoac029.

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Abstract STUDY QUESTION Is relative mitochondrial DNA (mtDNA) content in cumulus cells related to embryo developmental competence in humans and/or the bovine model? SUMMARY ANSWER mtDNA content in cumulus cells provides poor predictive value of oocyte developmental potential, both in vitro and following embryo transfer. WHAT IS KNOWN ALREADY Cumulus cells are closely connected to the oocyte through transzonal projections, serving essential metabolic functions during folliculogenesis. These oocyte-supporting cells are removed and discarded prior to ICSI, thereby providing interesting biological

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Martíne. Moro, Á., I. Lamas-Toranzo, L. González-Brusi, A. Pérez-Gómez, and P. Bermejo-Álvarez. "P–219 mtDNA content in bovine cumulus cells does not predict oocytés developmental competence." Human Reproduction 36, Supplement_1 (2021). http://dx.doi.org/10.1093/humrep/deab130.218.

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Abstract Study question Does cumulus cell mtDNA content correlate with oocyte developmental potential in the bovine model? Summary answer The relative amount of mtDNA content did not vary significantly in oocytes showing different developmental outcomes following IVF What is known already Cumulus cells are closely connected to the oocyte through transzonal projections, serving essential metabolic functions during folliculogenesis. These oocyte-supporting cells are removed and discarded prior to ICSI, thereby constituting an interesting biological material on which to perform molecular analysis

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Converse, Aubrey, T. Rajendra Kumar, and Francesca E. Duncan. "OR19-04 Hypoglycosylated FSH Enhances Ovarian Follicle Development and Gamete Quality." Journal of the Endocrine Society 7, Supplement_1 (2023). http://dx.doi.org/10.1210/jendso/bvad114.1650.

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Abstract Disclosure: A. Converse: None. T. Kumar: None. F.E. Duncan: None. Gamete quality depends on the proper growth and maturation of the ovarian follicle, which consists of the oocyte and its supporting somatic cells. FSH is essential for follicle growth as female Fshb and Fshr null mice are infertile due to arrested follicle development. Macroheterogeneity in N-glycosylation on the FSHß-subunit confers differential bioactivities with hypoglycosylated FSH21 exhibiting higher FSHR binding affinity and bioactivity compared to fully glycosylated FSH24. In females, there is a shift from the hi

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Kumar, K., L. Nesbeth, M. Venturas, D. Needleman, C. Racowsky, and D. Wells. "P-175 Human cumulus cell telomere length and its association with assisted reproduction outcomes." Human Reproduction 38, Supplement_1 (2023). http://dx.doi.org/10.1093/humrep/dead093.535.

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Abstract Study question Is there any relationship between the relative telomere length (RTL) within cumulus cells (CCs) and the outcome of assisted reproductive treatment using the corresponding oocyte? Summary answer Lower RTLs in CCs were significantly associated with embryos chosen for transfer or cryopreservation. In contrast, embryos considered non-viable (discarded) tended to have higher RTLs. What is known already Cumulus cells fulfil vital roles in support of oocyte development, including the transduction of external signals and the provision of resources via transzonal projections. Gi

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Dadashzadeh, Arezoo, Saeid Moghassemi, Alexis Peaucelle, Carolina M. Lucci, and Christiani A. Amorim. "Mind the mechanical strength: tailoring a 3D matrix to encapsulate isolated human preantral follicles." Human Reproduction Open, February 17, 2023. http://dx.doi.org/10.1093/hropen/hoad004.

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Abstract STUDY QUESTION Would a hydrogel with similar mechanical properties to the human ovarian cortex support preantral follicle development? SUMMARY ANSWER Yes, our tailored PEGylated fibrin hydrogel was shown to significantly improve follicle growth in vitro. WHAT IS KNOWN ALREADY One of the main challenges in developing an engineered ovary is to provide a 3D matrix that supports the follicle architecture and the interaction between granulosa cells and the oocyte as they are essential for folliculogenesis. Thanks to its biocompatibility and bioactivity, fibrin has been employed to fabricat

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