Relevant bibliographies by topics / Transporteur ABC

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Transporteur ABC'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 November 2024

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Transporteur ABC.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Transporteur ABC"

1

Mosser, J., CO Sarde, JL Mandel, AM Douar, and P. Aubourg. "Le gène de l'adrénoleucodystrophie pourrait coder pour un transporteur ABC." médecine/sciences 9, no. 3 (1993): 319. http://dx.doi.org/10.4267/10608/2915.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

ZHAO, Li-Xia, Cheng-Ji ZHOU, Arowu TANAKA, Masanori NAKATA, Takahiro HIRABAYASHI, Teruo AMACHI, Seiji SHIODA, Kazumitsu UEDA, and Nobuya INAGAKI. "Cloning, characterization and tissue distribution of the rat ATP-binding cassette (ABC) transporter ABC2/ABCA2." Biochemical Journal 350, no. 3 (September 8, 2000): 865–72. http://dx.doi.org/10.1042/bj3500865.

Full text
Abstract:
The ABC1 (ABCA) subfamily of the ATP-binding cassette (ABC) transporter superfamily has a structural feature that distinguishes it from other ABC transporters. Here we report the cloning, molecular characterization and tissue distribution of ABC2/ABCA2, which belongs to the ABC1 subfamily. Rat ABC2 is a protein of 2434 amino acids that has 44.5%, 40.0% and 40.8% identity with mouse ABC1/ABCA1, human ABC3/ABCA3 and human ABCR/ABCA4 respectively. Immunoblot analysis showed that proteins of 260 and 250kDa were detected in COS-1 cells transfected with ABC2 having a haemagglutinin tag, while no band was detected in mock-transfected cells. After incubation with N-glycosidase F, the mobilities of the two proteins increased and a single band was detected, suggesting that ABC2 is a glycoprotein. Photoaffinity labelling with 8-azido-[α-32P]ATP confirmed that ATP binds to the ABC2 protein in the presence of Mg2+. RNA blot analysis showed that ABC2 mRNA is most abundant in rat brain. Examination of brain by in situ hybridization determined that ABC2 is expressed at high levels in the white matter, indicating that it is expressed in the oligodendrocytes. ABC2, therefore, is a glycosylated ABC transporter protein, and may play an especially important role in the brain. In addition, the N-terminal 60-amino-acid sequence of the human ABC1, which was missing from previous reports, has been determined.
APA, Harvard, Vancouver, ISO, and other styles
3

Väisänen, Enni, Junko Takahashi, Ogonna Obudulu, Joakim Bygdell, Pirkko Karhunen, Olga Blokhina, Teresa Laitinen, et al. "Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce." Journal of Experimental Botany 71, no. 20 (August 10, 2020): 6379–95. http://dx.doi.org/10.1093/jxb/eraa368.

Full text
Abstract:
Abstract Both the mechanisms of monolignol transport and the transported form of monolignols in developing xylem of trees are unknown. We tested the hypothesis of an active, plasma membrane-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from developing xylem and lignin-forming tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as from control materials, comprising non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested that this transport was through the tonoplast. Membrane vesicles prepared from lignin-forming spruce cells showed coniferin transport, but the Km value for coniferin was much higher than those of xylem and BY-2 cells. Liquid chromatography-mass spectrometry analysis of membrane proteins isolated from spruce developing xylem, phloem, and lignin-forming cultured cells revealed multiple transporters. These were compared with a transporter gene set obtained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for ABC-transporter-mediated monolignol transport but point to a role for secondary active transporters (such as MFS or MATE transporters). In contrast, proteomic and co-expression analyses suggested a role for ABC transporters and MFS transporters.
APA, Harvard, Vancouver, ISO, and other styles
4

Webb, Alexander J., and Arthur H. F. Hosie. "A Member of the Second Carbohydrate Uptake Subfamily of ATP-Binding Cassette Transporters Is Responsible for Ribonucleoside Uptake in Streptococcus mutans." Journal of Bacteriology 188, no. 23 (September 22, 2006): 8005–12. http://dx.doi.org/10.1128/jb.01101-06.

Full text
Abstract:
ABSTRACT Streptococcus mutans has a significant number of transporters of the ATP-binding cassette (ABC) superfamily. Members of this superfamily are involved in the translocation of a diverse range of molecules across membranes. However, the functions of many of these members remain unknown. We have investigated the role of the single S. mutans representative of the second subfamily of carbohydrate uptake transporters (CUT2) of the ABC superfamily. The genetic context of genes encoding this transporter indicates that it may have a role in ribonucleoside scavenging. Inactivation of rnsA (ATPase) or rnsB (solute binding protein) resulted in strains resistant to 5-fluorocytidine and 5-fluorouridine (toxic ribonucleoside analogues). As other ribonucleosides including cytidine, uridine, adenosine, 2-deoxyuridine, and 2-deoxycytidine protected S. mutans from 5-fluorocytidine and 5-fluorouridine toxicity, it is likely that this transporter is involved in the uptake of these molecules. Indeed, the rnsA and rnsB mutants were unable to transport [2-14C]cytidine or [2-14C]uridine and had significantly reduced [8-14C]adenosine uptake rates. Characterization of this transporter in wild-type S. mutans indicates that it is a high-affinity (Km = 1 to 2 μM) transporter of cytidine, uridine, and adenosine. The inhibition of [14C]cytidine uptake by a range of structurally related molecules indicates that the CUT2 transporter is involved in the uptake of most ribonucleosides, including 2-deoxyribonucleosides, but not ribose or nucleobases. The characterization of this permease has directly shown for the first time that an ABC transporter is involved in the uptake of ribonucleosides and extends the range of substrates known to be transported by members of the ABC transporter superfamily.
APA, Harvard, Vancouver, ISO, and other styles
5

Latif, Haythem, Merve Sahin, Janna Tarasova, Yekaterina Tarasova, Vasiliy A. Portnoy, Juan Nogales, and Karsten Zengler. "Adaptive Evolution of Thermotoga maritima Reveals Plasticity of the ABC Transporter Network." Applied and Environmental Microbiology 81, no. 16 (June 5, 2015): 5477–85. http://dx.doi.org/10.1128/aem.01365-15.

Full text
Abstract:
ABSTRACTThermotoga maritimais a hyperthermophilic anaerobe that utilizes a vast network of ABC transporters to efficiently metabolize a variety of carbon sources to produce hydrogen. For unknown reasons, this organism does not metabolize glucose as readily as it does glucose di- and polysaccharides. The leading hypothesis implicates the thermolability of glucose at the physiological temperatures at whichT. maritimalives. After a 25-day laboratory evolution, phenotypes were observed with growth rates up to 1.4 times higher than and glucose utilization rates exceeding 50% those of the wild type. Genome resequencing revealed mutations in evolved cultures related to glucose-responsive ABC transporters. The native glucose ABC transporter, GluEFK, has more abundant transcripts either as a result of gene duplication-amplification or through mutations to the operator sequence regulating this operon. Conversely, BglEFGKL, a transporter of beta-glucosides, is substantially downregulated due to a nonsense mutation to the solute binding protein or due to a deletion of the upstream promoter. Analysis of the ABC2 uptake porter families for carbohydrate and peptide transport revealed that the solute binding protein, often among the transcripts detected at the highest levels, is predominantly downregulated in the evolved cultures, while the membrane-spanning domain and nucleotide binding components are less varied. Similar trends were observed in evolved strains grown on glycerol, a substrate that is not dependent on ABC transporters. Therefore, improved growth on glucose is achieved through mutations favoring GluEFK expression over BglEFGKL, and in lieu of carbon catabolite repression, the ABC transporter network is modulated to achieve improved growth fitness.
APA, Harvard, Vancouver, ISO, and other styles
6

Michaelis, Martin, Florian Rothweiler, Thomas Nerreter, Mohsen Sharifi, Taravat Ghafourian, and Jindrich Cinatl. "Karanjin interferes with ABCB1, ABCC1, and ABCG2." Journal of Pharmacy & Pharmaceutical Sciences 17, no. 1 (March 10, 2014): 92. http://dx.doi.org/10.18433/j3bw2s.

Full text
Abstract:
PURPOSE: The prominent ATP-binding cassette (ABC) transporters ABCB1, ABCC1, and ABCG2 are involved in substance transport across physiological barriers and therefore in drug absorption, distribution, and elimination. They also mediate multi-drug resistance in cancer cells. Different flavonoids are known to interfere with different ABC transporters. Here, the effect of the furanoflavonol karanjin, a potential drug with antiglycaemic, gastroprotective, antifungal, and antibacterial effects, was investigated on ABCB1, ABCC1, and ABCG2-mediated drug transport in comparison to the flavonoids apigenin, genistein, and naringenin. METHODS: Cells expressing the relevant transporters (ABCB1: UKF-NB-3ABCB1, UKF-NB-3rVCR10; ABCC1: G62, PC-3rVCR20; ABCG2: UKF-NB-3ABCG2) were used in combination with specific fluorescent and cytotoxic ABC transporter substrates and ABC transporter inhibitors to study ABC transporter function. Moreover, the effects of the investigated flavonoids were determined on the ABC transporter ATPase activities. RESULTS: Karanjin interfered with drug efflux mediated by ABCB1, ABCC1, and ABCG2 and enhanced the ATPase activity of all three transporters. Moreover, karanjin exerted more pronounced effects than the control flavonoids apigenin, genistein, and naringenin on all three transporters. Most notably, karanjin interfered with ABCB1 at low concentrations being about 1µM. CONCLUSIONS: Taken together, these findings should be taken into account during further consideration of karanjin as a potential drug for different therapeutic indications. The effects on ABCB1, ABCC1, and ABCG2 may affect the pharmacokinetics of co-administered drugs. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.METHODS: Cells expressing the relevant transporters (ABCB1: UKF-NB-3ABCB1, UKF-NB-3rVCR10; ABCC1: G62, PC-3rVCR20; ABCG2: UKF-NB-3ABCG2) were used in combination with specific fluorescent and cytotoxic ABC transporter substrates and ABC transporter inhibitors to study ABC transporter function. Moreover, the effects of the investigated flavonoids were determined on the ABC transporter ATPase activities.RESULTS: Karanjin interfered with drug efflux mediated by ABCB1, ABCC1, and ABCG2 and enhanced the ATPase activity of all three transporters. Moreover, karanjin exerted more pronounced effects than the control flavonoids apigenin, genistein, and naringenin on all three transporters. Most notably, karanjin interfered with ABCB1 at low concentrations being about 1µM.CONCLUSIONS: Taken together, these findings should be taken into account during further consideration of karanjin as a potential drug for different therapeutic indications. The effects on ABCB1, ABCC1, and ABCG2 may affect the pharmacokinetics of co-administered drugs.
APA, Harvard, Vancouver, ISO, and other styles
7

Schoonbeek, Henk-jan, Jos M. Raaijmakers, and Maarten A. De Waard. "Fungal ABC Transporters and Microbial Interactions in Natural Environments." Molecular Plant-Microbe Interactions® 15, no. 11 (November 2002): 1165–72. http://dx.doi.org/10.1094/mpmi.2002.15.11.1165.

Full text
Abstract:
In natural environments, microorganisms are exposed to a wide variety of antibiotic compounds produced by competing organisms. Target organisms have evolved various mechanisms of natural resistance to these metabolites. In this study, the role of ATP-binding cassette (ABC) transporters in interactions between the plant-pathogenic fungus Botrytis cinerea and antibiotic-producing Pseudomonas bacteria was investigated in detail. We discovered that 2,4-diacetylphloroglucinol, phenazine-1-carboxylic acid and phenazine-1-carboxamide (PCN), broad-spectrum antibiotics produced by Pseudomonas spp., induced expression of several ABC transporter genes in B. cinerea. Phenazines strongly induced expression of BcatrB, and ΔBcatrB mutants were significantly more sensitive to these antibiotics than their parental strain. Treatment of B. cinerea germlings with PCN strongly affected the accumulation of [14C]fludioxonil, a phenylpyrrole fungicide known to be transported by BcatrB, indicating that phenazines also are transported by BcatrB. Pseudomonas strains producing phenazines displayed a stronger antagonistic activity in vitro toward ΔBcatrB mutants than to the parental B. cinerea strain. On tomato leaves, phenazine-producing Pseudomonas strains were significantly more effective in reducing gray mold symptoms incited by a ΔBcatrB mutant than by the parental strain. We conclude that the ABC transporter BcatrB provides protection to B. cinerea in phenazine-mediated interactions with Pseudomonas spp. Collectively, these results indicate that fungal ABC transporters can play an important role in antibiotic-mediated interactions between bacteria and fungi in plant-associated environments. The implications of these findings for the implementation and sustainability of crop protection by antagonistic microorganisms are discussed.
APA, Harvard, Vancouver, ISO, and other styles
8

Ogawa, Atsuko, Takashi Hashida-Okado, Masahiro Endo, Hirofumi Yoshioka, Takashi Tsuruo, Kazutoh Takesako, and Ikunoshin Kato. "Role of ABC Transporters in Aureobasidin A Resistance." Antimicrobial Agents and Chemotherapy 42, no. 4 (April 1, 1998): 755–61. http://dx.doi.org/10.1128/aac.42.4.755.

Full text
Abstract:
ABSTRACT Aureobasidin A (AbA) has strong antifungal effects arising from an unusual mechanism. We show that AbA interacts with ATP-binding cassette (ABC) transporters in yeast and mammalian cells. We isolated a gene ofSaccharomyces cerevisiae that conferred resistance to AbA when the gene was present in multiple copies. The gene was identical toYOR1/YRS1, which confers resistance to oligomycin, reveromycin, and organic anions, none of which have structures similar to that of AbA. We also isolated an aur3 Rrecessive mutant of S. cerevisiae with increased resistance to AbA. Northern hybridization showed that theaur3 R mutant expressed not onlyYOR1 but also the ABC transporter-encoding genePDR5 at high levels. Genetic studies showed that theaur3 R mutant had a mutation in thePDR1 gene, which encodes a transcriptional regulator ofPDR5 and YOR1. Analysis of a yor1disruptant of the aur3/pdr1 mutant showed that both the functional YOR1 gene and the mutation in PDR1were necessary for AbA resistance. These results suggest thatYOR1 is more important than PDR5 for AbA resistance. We found in Candida albicans a novel gene whose sequence was similar to the sequence of YOR1 in S. cerevisiae. The amino acid sequence of the C. albicans YOR1 homolog showed no significant similarity to the sequences ofCDR1 and CDR2, which are ABC transporters ofC. albicans. Furthermore, AbA inhibited the efflux of the anticancer agent vincristine through P glycoproteins in cancer cells with multidrug resistance.
APA, Harvard, Vancouver, ISO, and other styles
9

Zhang, Wandong, Qing Yan Liu, Arsalan S. Haqqani, Ziying Liu, Caroline Sodja, Sonia Leclerc, Ewa Baumann, Christie E. Delaney, Eric Brunette, and Danica B. Stanimirovic. "Differential Expression of ABC Transporter Genes in Brain Vessels vs. Peripheral Tissues and Vessels from Human, Mouse and Rat." Pharmaceutics 15, no. 5 (May 22, 2023): 1563. http://dx.doi.org/10.3390/pharmaceutics15051563.

Full text
Abstract:
Background: ATP-binding cassette (ABC) transporters comprise a superfamily of genes encoding membrane proteins with nucleotide-binding domains (NBD). These transporters, including drug efflux across the blood–brain barrier (BBB), carry a variety of substrates through plasma membranes against substrate gradients, fueled by hydrolyzing ATP. The expression patterns/enrichment of ABC transporter genes in brain microvessels compared to peripheral vessels and tissues are largely uncharacterized. Methods: In this study, the expression patterns of ABC transporter genes in brain microvessels, peripheral tissues (lung, liver and spleen) and lung vessels were investigated using RNA-seq and WesTM analyses in three species: human, mouse and rat. Results: The study demonstrated that ABC drug efflux transporter genes (including ABCB1, ABCG2, ABCC4 and ABCC5) were highly expressed in isolated brain microvessels in all three species studied; the expression of ABCB1, ABCG2, ABCC1, ABCC4 and ABCC5 was generally higher in rodent brain microvessels compared to those of humans. In contrast, ABCC2 and ABCC3 expression was low in brain microvessels, but high in rodent liver and lung vessels. Overall, most ABC transporters (with the exception of drug efflux transporters) were enriched in peripheral tissues compared to brain microvessels in humans, while in rodent species, additional ABC transporters were found to be enriched in brain microvessels. Conclusions: This study furthers the understanding of species similarities and differences in the expression patterns of ABC transporter genes; this is important for translational studies in drug development. In particular, CNS drug delivery and toxicity may vary among species depending on their unique profiles of ABC transporter expression in brain microvessels and BBB.
APA, Harvard, Vancouver, ISO, and other styles
10

Kropf, Christian, Karl Fent, Stephan Fischer, Ayako Casanova, and Helmut Segner. "ABC transporters in gills of rainbow trout (Oncorhynchus mykiss)." Journal of Experimental Biology 223, no. 15 (June 12, 2020): jeb221069. http://dx.doi.org/10.1242/jeb.221069.

Full text
Abstract:
ABSTRACTFish gills are a structurally and functionally complex organ at the interface between the organism and the aquatic environment. Gill functions include the transfer of organic molecules, both natural ones and xenobiotic compounds. Whether the branchial exchange of organic molecules involves active transporters is currently not known. Here, we investigated the presence, diversity and functional activity of ATP-binding cassette (ABC) transporters in gills of juvenile rainbow trout. By means of RT-qPCR, gene transcripts of members from the abcb, abcc and abcg subfamilies were identified. Comparisons with mRNA profiles from trout liver and kidney revealed that ABC transporters known to have an apical localization in polarized epithelia, especially abcc2 and abcb1, were under-represented in the gills. In contrast, ABC transporters with mainly basolateral localization showed comparable gene transcript levels in the three organs. The most prominent ABC transporter in gills was an abcb subfamily member, which was annotated as abcb5 based on the synteny and phylogeny. Functional in vivo assays pointed to a role of branchial ABC transporters in branchial solute exchange. We further assessed the utility of primary gill cell cultures to characterize transporter-mediated branchial exchange of organic molecules, by examining ABC transporter gene transcript patterns and functional activity in primary cultures. The gill cultures displayed functional transport activity, but the ABC mRNA expression patterns were different to those of the intact gills. Overall, the findings of this study provide evidence for the presence of functional ABC transporter activity in gills of fish.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Transporteur ABC"

1

Matar, Merheb Rachel Rima. "Caractérisation d’une nouvelle génération de détergents stabilisateurs des transporteurs abc en solution : cristallisation de BmrA, transporteur ABC bactérien." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10303.

Full text
Abstract:
En raison de leur résistance aux agents chimiothérapeutiques, les transporteurs ABC de phénotype MDR ont attiré l'attention de la communauté scientifique. Notre projet vise à trouver des conditions dans lesquelles les transporteurs ABC restent fonctionnels en solution pour aboutir à la cristallisation de ces protéines dans une conformation active. Dans ce but, nous avons conçu et développé une nouvelle classe de détergents, à base de calix[4]arène, qui stabilisent ces protéines. Afin de résoudre la structure 3D à résolution atomique du transporteur ABC bactérien "BmrA", responsable de la résistance aux antibiotiques, nous avons utilisé une approche classique utilisant des détergents commerciaux en parallèle à nos détergents innovants. En présence de la Foscholine 12, nous avons obtenu des cristaux diffractant jusqu’à 5 Å de résolution. Cependant, les données de diffraction n’étaient pas suffisantes pour déterminer la structure tridimensionnelle complète de la protéine, seuls les domaines transmembranaires ont été résolus. D'autre part, nous avons atteint l'objectif de l'extraction, la purification et la stabilisation de ce transporteur à l'aide des détergents à base de calix [4] arène. Nous avons également montré que ces détergents promeuvent et améliorent la cinétique de cristallisation de BmrA, une étape que nous sommes en train d’optimiser, pour obtenir des cristaux de meilleure résolution, pour résoudre la structure 3D de BmrA qui sera utilisé pour concevoir des inhibiteurs adaptés
Due to their preponderance in the resistance to chemotherapies, the MDR ABC transporters have drawn the attention of the scientific community. Our project aimed at finding conditions in which ABC transporters are active in solution to lead the crystallization of these proteins in an active conformation. In this purpose, we conceived and developed a new class of detergents, based on calix[4]arene ring, that stabilize these proteins. In order to solve the 3D-structure to atomic resolution of bacterial ABC transporter “BmrA” responsible for antibiotic resistance, we used a classical approach with commercial detergents in addition to the innovative ones. We have crystallized the protein in presence of Foscholine 12 with a diffraction resolution up to 5 Å. The data was incomplete; solving partially the structure of the transmembrane domains. On the other hand, we have reached the objective of extraction, purification and stabilization of this transporter by using calix[4]arene-based detergents. We have also shown that these detergents promote and enhance the kinetics of crystallization of BmrA, a step that we are improving, to get crystals of better resolution, for resolving the BmrA 3D-structure which will be used to design adapted inhibitors
APA, Harvard, Vancouver, ISO, and other styles
2

Vorac, Jaroslav. "Le fonctionnement du transporteur ABC de Streptococcus pneumoniae impliqué dans la résistance contre les peptides antimicrobiens." Thesis, Université Grenoble Alpes (ComUE), 2016. http://www.theses.fr/2016GREAV009/document.

Full text
Abstract:
Streptococcus pneumoniae, le pneumocoque, est un pathogène majeur causant plus d'un million de morts par an dans le monde. De plus en plus de souches de pneumocoques sont résistants aux antibiotiques, en faisant un problème majeur de santé publique dans le monde. Une partie des ces antibiotiques sont les peptides anti-microbiens (AMP), qui sont produit aussi bien par l'hôte que des bactéries pathogènes en tant que premier système de défense. On trouve dans le pneumocoque un transporteur ABC (ATP-Binding Cassette) lié à un système de deux composants (TCS) – la kinase d'histidine (HK) et le régulateur de réponse (RR), qui cible les AMP. Récemment, il a été démontré, que l'absence du transporteur ABC augmente la sensibilité à la bacitracine. Dans ce projet, nous avons essayé à comprendre le mécanisme fonctionnel entre le transporteur ABC et TCS en utilisant des outils in vivo et in vitro
Streptococcus pneumoniae, the pneumococcus, is a major human pathogen causing over a million deaths each year. Many pneumococcal strains display resistance towards antibiotics causing world-wide health concern. Some of these antibiotics are antimicrobial peptides (AMP), which are produced as a primary defense by hosts as well as pathogens. The pneumococcus harbors a system comprised of an ATP-binding cassette (ABC) transporter and a two-component system (TCS) composed of a histidine kinase (HK) and a response regulator (RR), which targets these molecules. It has been shown recently that the removal of this ABC transporter increases the sensitivity of the bacteria towards bacitracin. In this project, we tried to understand the functioning mechanism of the ABC transporter and the co-operation with the TCS using both in vivo and in vitro techniques
APA, Harvard, Vancouver, ISO, and other styles
3

Mathieu, Khadija. "Caractérisation d’un transporteur ABC d’antibiotiques de Streptococcus pneumoniae, PatA-PatB." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSE1052.

Full text
Abstract:
Au cours des dernières décennies, une augmentation non négligeable du phénomène de résistance aux antibiotiques des bactéries a été observée. Ces bactéries possèdent plusieurs mécanismes de résistance parmi lesquels l’utilisation de transporteurs de type MDR (MultiDrug Resistance) dont certains appartiennent à la famille des transporteurs ABC (ATP-Binding Cassette). Les transporteurs ABC sont des protéines membranaires et ubiquitaires qui possèdent une topologie commune avec deux domaines transmembranaires et deux domaines cytoplasmiques. Les transporteurs ABC de type exportateur permettent le transport de molécules à l’extérieur de la cellule en utilisant l’énergie fournie par l’hydrolyse de l’ATP. PatA-PatB est un transporteur ABC de Streptococcus pneumoniae, un pathogène humain responsable de pneumonies et de méningites. Cette protéine est impliquée dans la résistance de ce pathogène à des antibiotiques de types fluoroquinolones. Pour étudier son mécanisme moléculaire, nous avons optimisé la surexpression fonctionnelle de ce transporteur chez Escherichia coli. Ainsi, nous avons pu caractériser son activité de transport de drogues et son activité d’hydrolyse de nucléotides. Ces expériences ont révélé que PatA-PatB a la particularité d’utiliser préférentiellement le GTP comme source d’énergie, contrairement aux autres membres de cette famille. Afin d’identifier l’origine de cette propriété au niveau moléculaire, des expériences de mutagénèse dirigée ont été effectuées et nous avons ainsi identifié deux simples mutants qui transportent les drogues aussi bien avec du GTP que de l’ATP
The excessive use of antibiotics during the past decades led to the amplification of multidrug resistance in pathogenic bacteria. Bacteria have developed several mechanisms of antibiotic resistance. One of them involves the antibiotic efflux by MDR (MultiDrug Resistance) transporters, some of which belong to the ABC (ATP-Binding Cassette) transporter family. ABC transporter are ubiquitous membrane proteins with a conserved topology comprising four domains : two «TransMembrane Domain» and two cytoplasmic domains named « Nucleotide-Binding Domain ». ABC exporters expel drugs outside the bacteria using the energy of ATP hydrolysis. PatA-PatB is an ABC transporter from Streptococcus pneumoniae, a human pathogen bacterium responsible for pneumonia and meningitis. This protein is involved in S. pneumoniae resistance against fluoroquinolone antibiotics. To study the molecular mechanism, we optimized the functional expression of this transporter in Escherichia coli. Then, we characterized its drug transport activity and its nucleotide hydrolysis activity. These experiments showed that PatA-PatB, in contrast to other members of the ABC superfamily, preferentially uses GTP as energy supply. To identify the origin of this property at a molecular level, mutagenesis experiments were performed and we identified two mutants capable of an even drug transport with ATP and GTP
APA, Harvard, Vancouver, ISO, and other styles
4

Lakli, Mounia. "Pharmacothérapie ciblée de variants d'ABCB4, le transporteur biliaire de phospholipides." Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPASQ026.

Full text
Abstract:
ABCB4/MDR3 est une protéine transmembranaire qui assure la sécrétion de la phosphatidylcholine, un composant fondamental de la bile, au niveau de la membrane canaliculaire des hépatocytes. De nombreuses mutations qui touchent le gène encodant ce transporteur sont à l'origine de pathologies cholestatiques rares. La cholestase intrahépatique familiale progressive de type 3 (PFIC3) représente la forme la plus sévère de ces maladies. À ce jour, environ 50 % des patients ne répondent pas aux traitements conventionnels, faisant de la transplantation hépatique l'ultime alternative thérapeutique pour ces jeunes patients. Cette thèse a eu pour objectif d'identifier par une approche de criblage à haut débit, de nouveaux correcteurs pharmacologiques du trafic intracellulaire de trois variants d'ABCB4 (I541F, L556R et I490T), retenus dans le réticulum endoplasmique. Les résultats des analyses biochimiques et morphologiques nous ont permis de valider, dans nos modèles cellulaires, trois correcteurs de la maturation et de la localisation membranaire de deux variants. Néanmoins, à cause d'un effet inhibiteur de ces molécules sur la fonction d'ABCB4, un seul correcteur a pu restaurer de manière significative la fonction de ces deux variants. En association avec l'ivacaftor (VX 770, Kalydeco®), un modulateur d'activité approuvé pour la mucoviscidose, une amélioration et une potentialisation de l'activité a été obtenue. Dans le but d'explorer le mécanisme d'action de ses composés, des analyses de docking moléculaire in silico ont été réalisées et suggèrent une interaction des drogues avec les résidus d'ABCB4 impliqués dans la liaison/hydrolyse de l'ATP expliquant potentiellement l'effet inhibiteur de la fonction. De plus, in vitro, les molécules augmentent la stabilité à la membrane plasmique d'ABCB4-WT et semblent inhiber sa dégradation lysosomale. De façon intéressante, l'effet correcteur de ces molécules est conservé pour un autre variant intracellulaire du transporteur d'acides biliaires ABCB11. Cela permet d'envisager un traitement consensus des déficits des deux transporteurs ABC, ABCB4 et ABCB11. Pour conclure, nous avons identifié de nouveaux composés correcteurs pour les variants d'ABCB4 retenus au niveau intracellulaire. Ces résultats ouvrent la voie à leur optimisation chimique afin de fournir de nouveaux médicaments candidats comme alternative potentielle à la transplantation hépatique pour les patients atteints de formes sévères de maladies liées aux déficits d'ABCB4
ABCB4/MDR3 is a transmembrane protein that secretes phosphatidylcholine, a fundamental component of bile, to the canalicular membrane of hepatocytes. Numerous mutations in the gene encoding this transporter are responsible for rare cholestatic diseases, the most severe one being progressive familial intrahepatic cholestasis type 3 (PFIC3). To date, at least 50 % of patients do not respond to conventional treatments, making liver transplantation the ultimate alternative therapy. Thus, this thesis was dedicated to characterizing and validating new pharmacological correctors for three traffic-defective ABCB4 variants (I541F, L556R and I490T) retained in the endoplasmic reticulum. In cell models, the biochemical and morphological analyses allowed us to identify three molecules able to rescue the maturation and canalicular localization of two variants. However, due to an inhibitory effect of these molecules on ABCB4 function, only one corrector was able to significantly restore the function of these variants. Combined with ivacaftor (VX 770, Kalydeco®), an approved modulator of activity for cystic fibrosis, an improvement and potentiation of ABCB4 activity was obtained. In silico molecular docking analyses were carried out to explore the mechanism of action of these compounds, suggesting an interaction of the drugs with ABCB4 residues involved in ATP binding/hydrolysis, which could explain the function inhibition effect. Furthermore, in vitro, the newly identified molecules increase the plasma membrane stability of ABCB4-WT and appear to inhibit its lysosomal degradation. Interestingly, the corrective effect of these molecules is conserved for an intracellular variant of the bile acid transporter ABCB11. This suggests the prospect of a consensus treatment for deficiencies of both ABC transporters. In conclusion, we have identified novel corrector compounds for intracellularly-retained ABCB4 variants. These results pave the way for their optimization to provide new drug candidates as potential alternatives to liver transplantation for patients with severe forms of ABCB4-related diseases
APA, Harvard, Vancouver, ISO, and other styles
5

Moulin, Pauline. "Caractérisation du transporteur de zinc Adc/Lmb de Streptococcus agalactiae." Thesis, Tours, 2017. http://www.theses.fr/2017TOUR3308/document.

Full text
Abstract:
Dans cette étude, le transporteur ABC de zinc de Streptococcus agalactiae, première cause d’infections materno-foetale en France, a été caractérisé. Nous avons montré que ce transporteur se compose, du complexe perméase-ATPase AdcCB, associé à trois protéines membranaires Lmb, AdcA et AdcAII redondantes dans la fixation de zinc. Ce transporteur comporte également deux protéines Sht et ShtII, retrouvées au niveau de la paroi, et nécessaires aux protéines Lmb et AdcAII pour la capture de zinc. L’absence d’un transporteur fonctionnel, par la triple délétion des gènes lmb, adcA et adcAII ou du complexe adcCB, a révélé une inhibition de la croissance et une perturbation de la division de la bactérie lorsqu’elle se trouve dans un environnement carencé en zinc. De plus, nous avons montré que ce transporteur de zinc participe à la survie de la bactérie en milieux biologiques humains, comme le liquide amniotique ou le LCR, où la bactérie est retrouvée lors d’infections, suggérant l’importance du transporteur lors du processus infectieux. Ces résultats ont mis en évidence, pour la première fois, que le zinc assure des fonctions biologiques vitales pour S. agalactiae et que, dans des conditions de forte carence en zinc, le transporteur Adc/Lmb représente le principal système d’acquisition de zinc de la bactérie
In this study, the zinc-ABC transporter of Streptococcus agalactiae, the first cause of materno-foetal infections in France, was characterized. We showed that this transporter is composed of an AdcCB permease-ATPase complex in association with three membrane-associated proteins Lmb, AdcA and AdcAII, which are redundant in zinc-binding. This transporter also possesses two proteins Sht and ShtII, which are associated to the cell wall, and that are necessary for the Lmb and AdcAII proteins for zinc capture. The absence of a functional transporter, by the triple deletion of the lmb, adcA and adcAII genes or the adcCB complex, revealed a growth inhibition and a disruption of the division of the bacterium when it is in a zinc-restricted environment. Furthermore, we showed that the zinc-ABC transporter contributes to the survival of the bacterium in human biological fluids, as the amniotic fluid or the cerebrospinal fluid, where the bacterium is found during infections, suggesting the importance of the transporter during the infectious process. These results hightlighted, for the first time, that zinc has biologically vital functions in S. agalactiae and that, under high zinc deficiency conditions, the Adc/Lmb transporter is the main zinc acquisition system of the bacterium
APA, Harvard, Vancouver, ISO, and other styles
6

Geillon, Flore. "Etude structure/fonction du demi-transporteur ABCD2 dans le contexte de l'Adrénoleucodystrophie liée à l'X." Thesis, Dijon, 2013. http://www.theses.fr/2013DIJOS067/document.

Full text
Abstract:
L’Adrénoleucodystrophie liée à l’X est une maladie neurodégénérative rare due à des mutations dans le gène ABCD1. Ce gène code un demi-transporteur ABC peroxysomal, impliqué dans l’importation d’acides gras à très longue chaîne. Deux autres demi-transporteurs sont localisés dans la membrane peroxysomale : ABCD2 et ABCD3. La surexpression d’ABCD2 permet de compenser la déficience en ABCD1, ouvrant ainsi des perspectives thérapeutiques. Dans cette optique, l’objectif principal de ma thèse était d’étudier la fonction et la structure d’ABCD2, et plus largement des transporteurs ABC peroxysomaux.Les demi-transporteurs doivent au minimum se dimériser pour constituer un transporteur fonctionnel. Leur dimérisation alternative pourrait moduler leur spécificité de substrat. Afin de tester cette hypothèse, nous avons réalisé des constructions plasmidiques codant différents dimères chimériques, dont la fonctionnalité a été vérifiée par transfection transitoire dans deux modèles cellulaires (fibroblastes humains et levures). D’après nos résultats, ABCD1 et ABCD2 seraient fonctionnels quel que soit leur agencement dimérique. De plus, comme d’autres transporteurs ABC, les transporteurs ABC peroxysomaux pourraient s’oligomériser. En utilisant différentes techniques biochimiques (co-immunoprécipitation, sédimentation sur gradient de sucrose et électrophorèse en conditions natives), sur un modèle cellulaire surexprimant ABCD2-EGFP, nous démontrons qu’ABCD2-EGFP interagit avec ABCD1 et ABCD3, et que les transporteurs ABC peroxysomaux sont capables de s’oligomériser. Il reste désormais à déterminer les facteurs qui contrôlent cette oligomérisation et comprendre la valeur fonctionnelle de ces interactions
X-linked Adrenoleukodystrophy (X-ALD) is a rare neurodegenerative disease caused by deficiency of the peroxisomal half-transporter ABCD1, implicated in very long chain fatty acids import. Two additional half-transporters are located in the peroxisomal membrane: ABCD2 and ABCD3. Over-expression of ABCD2 is known to compensate for ABCD1 deficiency, making ABCD2 a therapeutic target for X-ALD treatment. In this context, the main objective of my thesis was to investigate the function and the structure of ABCD2, and more broadly, of peroxisomal ABC transporters.Half-transporters must at least dimerize to form a functional transporter. Alternative dimerization could modulate substrate specificity. In order to test this hypothesis, we engineered plasmidic constructs encoding chimeric ABCD dimers, whose functionality has been evaluated by transient transfection in two cell models (human fibroblasts and yeasts). Our results show that, ABCD1 and ABCD2 are functional whatever their dimeric organization. Besides, like other ABC transporters, peroxisomal ABC transporters could oligomerize. By using a multi-technical approach (co-immunoprecipitation, velocity sucrose gradient and native polyacrylamide gel electrophoresis experiments) on stably transfected hepatoma cells expressing ABCD2-EGFP, we demonstrate that ABCD2-EGFP interacts with ABCD1 and ABCD3, and that peroxisomal ABC transporters oligomerize. The perspectives will consist in determining which factors control the oligomerization process and understanding the functional value of these interactions
APA, Harvard, Vancouver, ISO, and other styles
7

Cescau, Sandra. "Sécrétion de l'hémophore HasA de Serratia marcescens via un transporteur ABC." Paris 7, 2007. http://www.theses.fr/2007PA077213.

Full text
Abstract:
Chez les bactéries à Gram négatif, la voie de sécrétion ABC (T1SS) permet l'export de protéines présentant un signal de sécrétion C-terminal non clivable. Les transporteurs sont constitués de 3 protéines membranaires : une ATPase de la famille largement répandue des protéines ABC, une deuxième protéine de membrane interne et une protéine de membrane externe de la famille de TolC. TolC participe également aux pompes d'efflux de détergents et d'antibiotiques, les T1SS et les pompes d'efflux co-exprimés se partageant TolC sans perte de fonctionnalité. Le complexe de sécrétion n'est pas associé de façon permanente. Sa formation est induite par l'interaction entre le signal de sécrétion et la protéine ABC. L'oligomérisation du transporteur a été étudié par des approches biochimiques : chromatographie d'affinité et pontage chimique. Les mécanismes moléculaires permettant l'association et la dissociation du transporteur sont inconnus. Lors de ces travaux, le T1SS modèle utilisé est celui de l'hémophore HasA de S. Marcescens. Nous avons montré que HasA dépourvu de son signal C-terminal induit une oligomérisation stable du transporteur, séquestrant la protéine TolC. La pénurie de molécules TolC disponibles pour l'association aux pompes d'efflux entraîne une sensibilité accrue au SDS. L'hyperproduction de la protéine TolC réverse le phénotype de sensibilité. L'expression du signal de sécrétion sous forme de polypeptide distinct restaure aussi la résistance montrant que le signal C-terminal est actif de façon intermoléculaire. Ainsi, l'hémophore a deux domaines d'interaction avec la protéine ABC : le signal C- terminal et un 2eme domaine dit domaine d'ancrage
The Type I secretion System makes it possible the Gram negative bacteria to export proteins presenting an uncleaved C-terminal secretion signal. The transporter are constituted of 3 proteins: a membrane ATPase of the large family of ABC proteins, a second cytoplasmic membrane protein and an outer membrane protein belonging to TolC family. TolC is multifunctional. It participates also to efflux pump which expulse detergents and antibiotics. When they are co-expressed, T1SS and efflux pump share TolC without lost of functionality. The secretion complex is not permanently associated. Its formation is induced by the interaction between the secretion signal and the ABC protein. The oligomerisation of the transporter has been studied by several biochemical approaches: affinity chromatography and cross-linking. Th molecular mechanisms of the association-dissociation of the transporter are unknown. During this work, the model studied was the T1SS of the HasA hemophore of S. Marcescens. We have shown that Has deleted for its C-terminal secretion signal induced a stable oligomerisation of the transporter, trapping TolC proteins. The unavailability of TolC molecules for the efflux pump involved a increased SDS sensitivity. The hyperproduction of the TolC protein reversed this phenotype. The expression of the secretion signal as a single molecule also restored the resistance This suggests that the secretion signal is active in an intermolecular manner. Thus, the hemophore presents 2 interaction domains with the ABC protein: the secretion signal and a second site name the anchoring domain
APA, Harvard, Vancouver, ISO, and other styles
8

Paik, Su-Jin. "Couplages entre un transporteur membranaire de type ABC, BmrA et son environnement membranaire." Electronic Thesis or Diss., Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLET010.

Full text
Abstract:
Les ABC (ATP binding cassettes) transporteurs constituent une grande famille de protéine transmembranaire présentent dans tous les organismes. Les ABC hydrolysent l'ATP pour transporter une quantité immense de substrats amphiphiles, comme les lipides, steroids, peptides... Certains ABCs confèrent un phénotype cellulaire de multiresistance aux drogues des bactéries contre les antibiotiques à l’homme contre les agents anticancéreux, antiviraux ...Une question fondamentale pour comprendre le transport de drogues est comment les propriétés de membranes modulent la fonction et l’organisation spatio temporelle des ABCs transporteurs. Nous avons étudié en détails ces couplage avec BmrA, un ABC bactérien de B. subtillis en utilisant différents systèmes membranaires in vitro et différentes approches de biochimie et de biophysique membranaire. Dans un premier temps, après expression et purification des protéines en détergent, nous avons caractérisé l’hydrolyse d’ATP en fonction de l’état de l’environnement membranaire, solubilisé en micelle de détergent et en micelles mixtes. Les proteoliposomes ont été caractérisés en fonction de l’orientation des protéines, leur taux d’incorporation, leur taille et la lamellarité. Cela nous a permis de moduler de façon contrôlée la composition lipidique, la densité et la conformation des protéines et la courbure membranaire pour déterminer de façon quantitative les contributions respectives de ces paramètres de membranes. Ainsi, nous montrons que l’hydrolyse d’ATP est sensible à la spécificité lipidique quand la protéine est dans une bicouche et que les lipides négatifs et les lipides de type phosphatidylethanolamine stimulent de façon synergique l’activité hydrolytique. L’hydrolyse d’ATP diminue pour les fortes courbure positives. Dans un second temps, nous avons déterminé les conditions de reconstitution de BmrA dans des vésicules géantes qui ont ensuite permis d’étudier les rôles respectifs de la courbure et de la tensions membranaire dans l’organisation spatiale de BmrA par des approches de nanotube pulling. Les expériences en collaboration montrent que BmrA a une préférence marquée pour les régions membranaires à forte courbure conduisant à la formation de cluster de protéines et que cette préférence varie en fonction de l’état catalytique de la protéine. Finalement, nous avons mis au point une méthode pour étudier la dynamique des NBDs par transfert d'énergie de résonance de Förster au niveau de la molécule unique dans un système reconstitué par spectroscopie de fluorescence et de corrélation croisée.L’ensemble des données suggère que organisations spatiales des ABC transporteurs dans les cellules bactériennes et eucaryotes sont différentes avec la possibilité de tris dans des zones de fortes courbures lors de remodelages membranaires mais sans modification importante de la fonction
ABCs (ATP binding cassettes) transporters constitute a large family of transmembrane proteins present in all organisms. ABC transporters hydrolyze ATP to translocate an immense quantity of amphiphilic substrates, such as lipids, steroids, peptides... Some ABCs confer a multiresistance cellular phenotype to drugs from bacteria against antibiotics to humans against anticancer agents, antivirals...A fundamental question for understanding drug transport at the molecular level is how the properties of membranes modulate the function and spatial temporal organization of ABCs. We studied in detail these coupling with BmrA, a bacterial ABC of B. subtillis using different in vitro membrane systems and different biochemical and membrane biophysical approaches. Firstly, after expression and purification of proteins in detergent, we characterized the hydrolysis of ATP of BmrA according to its membrane environment, solubilized in detergent micelles and in mixed lipid/detergent micelles. Proteoliposomes were characterized according to protein orientation, incorporation rate, size and lamellarity. This allowed us to modulate in a controlled manner lipid composition, protein density and conformation and membrane curvature to quantitatively determine the respective contributions of these membrane parameters. Thus, we show that ATP hydrolysis is sensitive to lipid specificity when the protein is embedded in a bilayer. This lipid specificity is provided by negative lipids and phosphatidylethanolamine type lipids that synergistically stimulate hydrolytic activity. However, ATP hydrolysis was decreased in high positive membrane curvature. Secondly, we determined the conditions of reconstitution of BmrA in Giant Unilamellar Vesicles, which then allowed our collaborator to study the respective roles of membrane curvature and tension in the spatial organization of BmrA. Nanotube pulling experiments performed in collaboration show that BmrA has a strong preference for highly curved membrane regions leading to protein cluster formation and that this preference varies according to the catalytic state of the protein. Finally, we developed a method to study the dynamics of NBDs by Förster resonance energy transfer at the single molecule level in reconstituted system via fluorescence cross-correlation spectroscopy.The data set suggest that spatial organizations of ABC transporters in bacterial and eukaryotic cells are different with the possibility of sorting during membrane remodeling of eukaryotic membranes in areas of strong membrane curvatures but without significant change in function
APA, Harvard, Vancouver, ISO, and other styles
9

Jeannesson, Elise Siest Gérard Visvikis-Siest Sophie. "Analyse génétique et transcriptomique du transporteur ABCB1 en physiopathologie cardiovasculaire." S. l. : Nancy 1, 2008. http://www.scd.uhp-nancy.fr/docnum/SCD_T_2008_0130_JEANNESSON.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Olivier, Maryline. "Rôle du transporteur ABCG1 dans l’homéostasie lipidique cellulaire : implications physiopathologiques chez l'homme." Paris 6, 2010. http://www.theses.fr/2010PA066733.

Full text
Abstract:
L’homéostasie lipidique cellulaire repose sur l’entrée et/ou la synthèse des lipides et la sortie et/ou l’utilisation des lipides cellulaires. L’athérosclérose et l’obésité sont deux maladies pour lesquelles le mécanisme pathogénique repose sur un dysfonctionnement de l’homéostasie lipidique de cellules clés conduisant à l’accumulation de lipides dans le macrophage et l’adipocyte respectivement. Les études sur les modèles cellulaires et les modèles murins ont permis d’identifier un rôle du transporteur ABCG1 dans l’homéostasie et les mécanismes de transport des lipides (phospholipides, cholestérol, oxystérols). Ces travaux ont pour but d’identifier un rôle physiologique du transporteur ABCG1 humain. L’analyse du rôle du transporteur membranaire ABCG1 dans l’homéostasie lipidique chez l’homme m’a permis de mettre en évidence que l’expression de ce transporteur était associée avec l’activité lipoprotéine lipase (LPL). De plus, l’étude des mécanismes cellulaires impliqués sous-jacents à cette association (génotype d’ABCG1-activité LPL) a montré qu’ABCG1 favorise le stockage cellulaire de triglycérides et est fortement associé avec le développement de l’obésité chez l’homme. Enfin, l’étude du rôle physiologique d’ABCG1 dans l’homéostasie lipidique de la cellule hépatique a permis de mettre en évidence que ce transporteur était impliqué dans la captation sélective d’esters de cholestérol associés aux HDL ainsi que dans l’efflux cellulaire de cholestérol libre dans la cellule hépatique humaine. L’ensemble de ces travaux a permis d’identifier pour la première fois un rôle physiologique du transporteur membranaire ABCG1 dans l’homéostasie lipidique cellulaire chez l’homme
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Transporteur ABC"

1

Geisler, Markus, ed. Plant ABC Transporters. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-06511-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Procko, Erik. General mechanisms for ABC transporters revealed by the transporter associated with antigen processing. Cambridge, Mass: Harvard University, 2008.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Boumendjel, Ahcne, Jean Boutonnat, and Jacques Robert, eds. ABC Transporters and Multidrug Resistance. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2009. http://dx.doi.org/10.1002/9780470495131.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

George, Anthony M., ed. ABC Transporters - 40 Years on. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-23476-2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Ahcène, Boumendjel, Boutonnat Jean, and Robert Jacques M. D, eds. ABC transporters and multidrug resistance. Hoboken, N.J: John Wiley & Sons, 2009.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

B, Holland I., ed. ABC proteins: From bacteria to man. Amsterdam: Academic Press, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Efferth, Thomas, ed. Resistance to Targeted ABC Transporters in Cancer. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-09801-2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Ponte-Sucre, Alicia. ABC transporters in microorganisms: Research, innovation and value as targets against drug resistance. Norfolk, UK: Caister Academic, 2009.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Geisler, Markus. Plant ABC Transporters. Springer, 2016.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Geisler, Markus. Plant ABC Transporters. Springer, 2014.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Transporteur ABC"

1

Lackner, K. J., and D. Peetz. "ABC-Transporter." In Springer Reference Medizin, 3–4. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_59.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Lackner, K. J., and D. Peetz. "ABC-Transporter." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_59-1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Lage, Hermann. "ABC-Transporters." In Encyclopedia of Cancer, 1–5. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27841-9_13-5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Scherrmann, Jean-Michel, Kim Wolff, Christine A. Franco, Marc N. Potenza, Tayfun Uzbay, Lisiane Bizarro, David C. S. Roberts, et al. "ABC Transporters." In Encyclopedia of Psychopharmacology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1099.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Bates, Susan E., and Tito Fojo. "ABC Transporters." In Handbook of Anticancer Pharmacokinetics and Pharmacodynamics, 267–88. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1007/978-1-59259-734-5_17.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Lage, Hermann. "ABC-Transporters." In Encyclopedia of Cancer, 17–21. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-46875-3_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Lage, Hermann. "ABC-Transporters." In Encyclopedia of Cancer, 10–13. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-16483-5_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Grube, Markus, and Gabriele Jedlitschky. "ABC Transporters." In Encyclopedia of Molecular Pharmacology, 1–7. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-21573-6_174-1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Grube, Markus, and Gabriele Jedlitschky. "ABC Transporters." In Encyclopedia of Molecular Pharmacology, 1–7. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-57401-7_174.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Xu, YanXia, and YanHua Qi. "Monocot ABC Transporters." In Signaling and Communication in Plants, 203–17. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-06511-3_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Transporteur ABC"

1

LIMA, GABRIELA FERNANDES, Luana Neves clementino da Silva, Erik Nascimento de Carvalho, Bárbara Louise Lemos Drumond Silva, Letícia Vitorazi, and Newton Narciso Pereira. "CONTROLE DE EMISSÃO DE PARTICULADOS NO TRANSPORTE FERROVIÁRIO DE MINÉRIO DE FERRO: USO DE FILMES POLIMÉRICOS BIODEGRADÁVEIS." In 77º Congresso Anual da ABM - Internacional, 2354–63. São Paulo: Editora Blucher, 2024. http://dx.doi.org/10.5151/2594-5327-41201.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

LOCHER, KASPAR. "STRUCTURES AND REACTION MECHANISMS OF ABC TRANSPORTERS." In 23rd International Solvay Conference on Chemistry. WORLD SCIENTIFIC, 2014. http://dx.doi.org/10.1142/9789814603836_0030.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Pecks, U., L. Schmieding, Y. Sawierucha, K. Lüchow, N. Maass, and W. Rath. "LXR und ABC-Transporter-Expression im Trophoblast bei IUGR." In 62. Kongress der Deutschen Gesellschaft für Gynäkologie und Geburtshilfe – DGGG'18. Georg Thieme Verlag KG, 2018. http://dx.doi.org/10.1055/s-0038-1671434.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Schmieding, L., Y. Sawierucha, K. Lüchow, L. Segger, N. Maass, W. Rath, and U. Pecks. "LXR und ABC-Transporter-Expression im Trophoblast bei IUGR." In 28. Deutscher Kongress für Perinatale Medizin. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1607686.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Ettouati, Laurent, Marie-Emmanuelle Million, Ophélie Arnaud, Géraldine Agusti, Waël Zeinyeh, Lucia Gonzalez-Lobato, Ali Koubeissi, et al. "Advances in peptidomimetics as inhibitors of ABC transporters." In 1st International Electronic Conference on Medicinal Chemistry. Basel, Switzerland: MDPI, 2015. http://dx.doi.org/10.3390/ecmc-1-a043.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

KELLY, LIBUSHA, RACHEL KARCHIN, and ANDREJ SALI. "PROTEIN INTERACTIONS AND DISEASE PHENOTYPES IN THE ABC TRANSPORTER SUPERFAMILY." In Proceedings of the Pacific Symposium. WORLD SCIENTIFIC, 2006. http://dx.doi.org/10.1142/9789812772435_0006.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Huynh, Tony, Amanda Tivnan, Marcia Munoz, Leanna Cheung, Anasuya Vishvanath, Claudia Flemming, Fujiko Watt, et al. "Abstract 1834: Targeting ABC transporters in cancer through small molecule inhibitors." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-1834.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Matsui, Hirofumi, and Hiromi Kurokawa. "Abstract 1285A: Erythropoietin can cancelchemo-resistances viadown regulation of ABC transporters." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-1285a.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Matsui, Hirofumi, and Hiromi Kurokawa. "Abstract 1285A: Erythropoietin can cancelchemo-resistances viadown regulation of ABC transporters." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-1285a.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Durmus, Selvi, M. A. van der Valk, S. F. Teunissen, Els Wagenaar, Jos Beijnen, and Alfred H. Schinkel. "Abstract 4419: The role of ABC transporters in PhIP-induced colon carcinogenesis." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-4419.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Transporteur ABC"

1

Montoya, Valentina, Laureen Montes, Valeria Bernal, Gibet Camós,, Fanny Bertossi, Ángela María Orozco, Edith Arístide, et al. ABC Género y Transporte. Inter-American Development Bank, November 2021. http://dx.doi.org/10.18235/0003772.

Full text
Abstract:
Para nadie es un secreto que la movilidad es un factor determinante para la calidad de vida de todas las personas, tanto en el contexto urbano como en el rural. En cada uno de ellos, la planificación y gestión de los servicios asociados a la infraestructura del transporte tienen como finalidad, además de conectar a los ciudadanos, facilitar y responder a las diferentes necesidades de las personas, es decir, mitigar los índices de segregación socioespacial haciendo más próximas las zonas residenciales, económicas y productivas que ofrecen estos territorios.
APA, Harvard, Vancouver, ISO, and other styles
2

Pasinelli, Piera, and Dena Jacob. Rethinking Drug Treatment Approaches in ALS by Targeting ABC Efflux Transporters. Fort Belvoir, VA: Defense Technical Information Center, December 2014. http://dx.doi.org/10.21236/ada615391.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Jenness, Mark, and Angus Murphy. Analysis of plant ABCB organic acid transporters (Final Report). Office of Scientific and Technical Information (OSTI), November 2020. http://dx.doi.org/10.2172/1719140.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Crotte Alvarado, Amado, Carina Arvizu, Isabel Granada, and Carlos Mojica. Apoyo al desarrollo de Sistemas Inteligentes de Transporte (ITS). Inter-American Development Bank, November 2017. http://dx.doi.org/10.18235/0006103.

Full text
Abstract:
Con el fin de fortalecer el desarrollo de los ITS en ALC, durante 2014-2016 el BID apoyó la elaboración de ocho estudios en siete países de la región. Esta publicación describe cada uno de los estudios realizados, y desarrollados en las siguientes áreas: i) conceptualización de proyectos; ii) desarrollo de estándares tecnológicos; y iii) preparación de planes nacionales/municipales.
APA, Harvard, Vancouver, ISO, and other styles
5

De Rus Mendoza, Ginés, Javier Campos Méndez, and Ofelia Cruz. Manual de evaluación económica de proyectos de transporte. Inter-American Development Bank, November 2006. http://dx.doi.org/10.18235/0009777.

Full text
Abstract:
El presente Manual de Evaluación Económica de Proyectos de Transporte se ha elaborado a partir de un objetivo general y de nueve principios que se desprenden del análisis económico y de las lecciones aprendidas en la práctica del análisis coste-beneficio (ACB) por agencias nacionales y supranacionales. El objetivo general de este Manual es dotar al Banco Interamericano de Desarrollo (BID) y a los gobiernos de sus países prestatarios de una herramienta de evaluación de proyectos de transporte que sea útil y consistente con los avances conceptuales de la teoría económica, con las lecciones aprendidas internacionalmente y con la necesidad de elegir entre proyectos alternativos, diseñados dentro de las limitaciones habituales de recursos materiales y humanos y de la incertidumbre, que caracterizan a los procesos de evaluación en el mundo real.
APA, Harvard, Vancouver, ISO, and other styles
6

Fioravanti, Reinaldo, Mariano Ansaldo, Eduardo Café, Xavier Fageda, and Andy Ricover. El transporte de carga aérea en América Latina y el Caribe: análisis integral del sector y recomendaciones de políticas públicas. Banco Interamericano de Desarrollo, November 2022. http://dx.doi.org/10.18235/0004583.

Full text
Abstract:
En los últimos 10 años y hasta 2019, el volumen del transporte aéreo de mercancías había mantenido una tendencia creciente en América Latina y el Caribe (ALC). Las medidas sanitarias adoptadas por los países de la región por cuenta del COVID-19 han impactado directamente en la actividad económica y en el sector transporte aéreo y aeropuertos de forma muy severa. Se ha observado, en muchos casos, una interrupción prolongada u oferta muy limitada de frecuencias de vuelos, con la consiguiente reducción de los volúmenes de transporte de pasajeros y de carga. La reducción en la capacidad de transporte en la bodega de aeronaves de pasajeros, y la crisis económica han afectado negativamente las importaciones y exportaciones de los países de ALC. Sin embargo, los operadores consultados perciben como contrapartida un crecimiento auspicioso en segmentos de carga no tan preponderantes en el pasado farmacéuticos, material médico y e-commerce que están induciendo la implementación de nuevas estrategias de servicio en las aerolíneas de carga y la redefinición de las funciones de los centros de carga del esquema aeroportuario de la región.
APA, Harvard, Vancouver, ISO, and other styles
7

Granada, Isabel, José Rodrigo Rendón, Raphaël Dewez, Manuel Rodriguez Porcel, Mariam Peña, Pier Saraceno, and Paola Ortiz. Infraestructura vial y MiPyMEs: un estudio de accesibilidad y nodos logísticos. Banco Interamericano de Desarrollo, December 2022. http://dx.doi.org/10.18235/0004602.

Full text
Abstract:
Las Micro, Pequeñas y Medianas Empresas (MiPyMEs) representan un sector de suma importancia para América Latina y el Caribe (ALC). En efecto, el 99.5% de las empresas de la región son MiPyMEs. Estas abarcan el 60% de la población ocupada y generan el 25% del producto interno bruto regional. A pesar de que el empleo informal y el autoempleo en microempresas están muy extendidos en los países de la región, es inevitable reconocer el peso que las MiPyMEs tienen en la economía de ALC. En este punto cabe mencionar que, al estudiar la relación transporte y MiPyMEs, no solo se consideran las MiPyMEs de Transporte, sino que el transporte -per se cumple una función habilitadora para MiPyMEs que pertenecen a otros sectores de la economía. En este sentido, tener una mayor comprensión de estas lógicas es clave para el desarrollo integral de la región.
APA, Harvard, Vancouver, ISO, and other styles
8

Sosa, Martín, Lynn Scholl, Juan Manuel Leaño, Michael Fleischmann, Cristian Navas, and Juan Pablo Benitez. El potencial del remote sensing y las políticas de reducción de emisiones como catalizadores de la renovación de la flota de transporte en Asunción, Paraguay. Inter-American Development Bank, May 2021. http://dx.doi.org/10.18235/0003304.

Full text
Abstract:
El sector de transporte genera contribuciones significativas de emisiones locales y globales de gases de efecto invernadero y también de material particulado. Los servicios de transporte masivo en buses presentan altas tasas de emisiones dado el tipo de combustible del cual dependen y, en el caso de América Latina y el Caribe (ALC) estas externalidades son aún más severas dados los rezagos de regulación del sector, la informalidad y la edad promedio muy alta de la flota vehicular. Este documento presenta a la utilización de la tecnología remote sensing como una herramienta para reducción de las emisiones contaminantes locales de vehículos de transporte de pasajeros del rea Metropolitana de Asunción, Paraguay. El documento parte de una presentación de casos de aplicación de remote sensing en varias ciudades del mundo y de la región de ALC y luego delinea los beneficios, consideraciones y recomendaciones de replicar la implementación del remote sensing en Paraguay. Se analiza el potencial de esta tecnología para identificar vehículos con tasas más altas de emisiones locales, y a partir de esta herramienta, conceptualizar y desarrollar un mecanismo de aplicación de normas de emisiones contaminantes en Paraguay, que por medio de un sistema de multas sustente un fondo para la renovación de flota del transporte.
APA, Harvard, Vancouver, ISO, and other styles
9

Navas Duk, Cristián, Ángelo Guevara Cué, Elías Rubinstein, and Richard Mix Vidal. Análisis del estado del arte y experiencias de gestión de demanda de transporte urbano en América Latina y el Caribe. Inter-American Development Bank, October 2020. http://dx.doi.org/10.18235/0002895.

Full text
Abstract:
En las últimas décadas, la tasa de motorización en los países de América Latina y el Caribe (ALC) se ha incrementado, principalmente, por el crecimiento de los niveles de ingreso de las personas, lo que ha generado aumentos tanto en niveles de congestión, como también en variables medioambientales tales como la polución y el ruido. Una de las respuestas a este crecimiento de la tasa de motorización ha sido el desarrollo de políticas con enfoque en la oferta. A través de estas se ha propuesto aumentar la infraestructura vial con el objetivo de mejorar las condiciones de flujo y desplazamiento del tráfico vial, disminuyendo así las condiciones de congestión. Este enfoque se ha traducido en obras de infraestructura, como el ensanchamiento de calles y el desarrollo de nuevas vías y autopistas. A pesar de estos esfuerzos, existe evidencia en la literatura de que este tipo de respuesta podría resultar insostenible en el largo plazo, por su alto costo y porque promueve el uso del automóvil particular, generando aumentos en la congestión y contaminación entre otras externalidades. Una de las soluciones que ha planteado la literatura es abordar la problemática a través de medidas que gestionen la demanda por el uso de la infraestructura, promoviendo así una utilización más eficiente de la oferta vial disponible. El desarrollo de medidas de gestión de la demanda (Transportation Demand Management, TDM por sus siglas en inglés) ha sido ampliamente reportado y discutido como política de transporte a nivel global, donde su implementación se ha concentrado en intervenciones como la restricción de circulación de vehículos (pico y placa), la tarificación vial por congestión, la redistribución del espacio vial (vías de uso exclusivo o reversibles), y los impuestos a la compra y operación de vehículos particulares, entre otras. ALC no ha estado al margen de la implementación de este tipo de medidas, con distintos niveles de éxito en su implementación y desarrollo. El presente documento considera una recopilación no exhaustiva de casos y experiencias de la aplicación de medidas de gestión de demanda, tanto en ALC como en otras ciudades del mundo, con el objetivo de compartir estas experiencias de políticas públicas de transporte para contribuir a su discusión para potenciales implementaciones a futuro. Adicionalmente, este trabajo se plantea como instrumento de guía, apoyo y consulta para los distintos stakeholders de transporte urbano de las ciudades de ALC.
APA, Harvard, Vancouver, ISO, and other styles
10

Castillo, Marcela, Sebastián Galarza S., Juanita Concha, Felipe García, Benoit Lefevre, Gibet Camós, Fanny Bertossi, and Paula Melisa Cruz Moreno. Desafíos operacionales y soluciones a la integración de buses eléctricos: lecciones de Cali, Colombia. Inter-American Development Bank, October 2021. http://dx.doi.org/10.18235/0003733.

Full text
Abstract:
Los últimos cinco años han presentado un cambio importante en los procesos de renovación de las flotas de sistemas de transporte público de ALC, donde los buses eléctricos representan una opción cada vez más viable para sustituir los buses del transporte colectivo convencional. En Colombia, este proceso comenzó con la integración de 26 buses eléctricos a la flota del SITM de Cali, siendo la primera ciudad del país en contar con una flota eléctrica para sus servicios de transporte público. Uno de los casos más emblemáticos la región, es la adjudicación de más de 1.400 unidades nuevas de buses eléctricos para el sistema de transporte público de Bogotá. Esto ha permitido generar una creciente experiencia de parte de autoridades, concesionarios y otros actores del transporte público con la operación y mantenimiento de flota eléctrica y sus sistemas de carga. Al mismo tiempo, estos procesos han permitido constatar las ventajas comparativas de la tecnología respecto a las operaciones de transporte público, pero también a identificar las brechas que se deben suplir para crear las condiciones propicias para una masificación de esta tecnología en el corto y mediano plazo.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Transporteur ABC' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography