Dissertations / Theses on the topic 'Transient receptor potential melastatin channels'
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Lucius, Alexander [Verfasser]. "Characterization of temperature-sensitive transient receptor potential channel melastatin 8 (TRPM8) in cultivated human ocular surface cells / Alexander Lucius." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2017. http://d-nb.info/1126503886/34.
Full textWang, Qian, and 王倩. "Mechanistic study of the transient receptor potential melastain 2 (TRPM2)-Ca²⁺ signaling in ROS induced switch between apoptosis and autophagy." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206750.
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Physiology
Doctoral
Doctor of Philosophy
Chen, Wenchun [Verfasser], and Bernhard [Gutachter] Nieswandt. "Studies on the role of calcium channels and the kinase domain of transient receptor potential melastatin-like 7 (TRPM7) in platelet function / Wenchun Chen. Gutachter: Bernhard Nieswandt." Würzburg : Universität Würzburg, 2014. http://d-nb.info/1111783284/34.
Full textPimentel, Montero Fátima Elizabeth. "Modulation of transient receptor potential melastatin 8 by protein kinase C /." Available to subscribers only, 2005. http://proquest.umi.com/pqdweb?did=1075689361&sid=10&Fmt=2&clientId=1509&RQT=309&VName=PQD.
Full textStokes, Alexander James. "Regulatory interactions of transient receptor potential channels." Thesis, University of Warwick, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418114.
Full textCao, De-Shou. "Role of transient receptor potential (TRP) channels in nociception /." Available to subscribers only, 2009. http://proquest.umi.com/pqdweb?did=1967913291&sid=2&Fmt=2&clientId=1509&RQT=309&VName=PQD.
Full textCao, Deshou. "Role of Transient Receptor Potential (TRP) Channels in Nociception." OpenSIUC, 2009. https://opensiuc.lib.siu.edu/dissertations/71.
Full textOgawa, Nozomi. "Detection of cellular redox status by transient receptor potential channels." 京都大学 (Kyoto University), 2016. http://hdl.handle.net/2433/215577.
Full textPabbidi, Reddy M. "Role of transient receptor potential channels in diabetic peripheral neuropathy /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1456284721&sid=5&Fmt=2&clientId=1509&RQT=309&VName=PQD.
Full textBomben, Valerie Christine. "Role of transient receptor potential canonical channels in glioma cell biology." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2010. https://www.mhsl.uab.edu/dt/2010p/bomben.pdf.
Full textGhavideldarestani, Maryam. "Role of transient receptor potential channels in mammalian oviduct and uterine epithelia." Thesis, University of Hull, 2011. http://hydra.hull.ac.uk/resources/hull:6068.
Full textWaddell, Trinity Q. "Role of Transient Receptor Potential Channels in Epithelial Morphogenesis in Chick Embryo." BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/8112.
Full textMergler, Stefan, Monika Valtink, Sumioka Takayoshi, Yuka Okada, Masayasu Miyajima, Shizuya Saika, and Peter S. Reinach. "Temperature-Sensitive Transient Receptor Potential Channels in Corneal Tissue Layers and Cells." Karger, 2014. https://tud.qucosa.de/id/qucosa%3A71636.
Full textKim, Ju Young. "M1 muscarinic acetylcholine receptor regulation of endogenous transient receptor potential-canonical, subtype 6 (TRPC6) channels." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1117570788.
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Sengupta, Sukanya. "Understanding the mechanisms of retinal degeneration in Drosophila lacking transient receptor potential channels." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609679.
Full textBaxter, Matthew. "The role of Transient Receptor Potential (TRP) channels in the pathogenesis of COPD." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/29840.
Full textChe, Hui, and 車慧. "Functional transient receptor potential channels in human preadipocytes and cardiac c-kit⁺ progenitor cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196436.
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Medicine
Doctoral
Doctor of Philosophy
Kato, Kenta. "Characterization of bioactive molecules using genetically engineered ion channels." 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120897.
Full textKatisart, Teeraporn. "Transient receptor potential function in bladder from control and streptozotocin treated rats." Thesis, University of Hertfordshire, 2011. http://hdl.handle.net/2299/6039.
Full textNi, Dan. "THERMAL SENSITIVITY OF VAGAL PULMONARY SENSORY NEURONS: ROLE OF TRANSIENT RECEPTOR POTENTIAL VANILLOID CHANNELS." Lexington, Ky. : [University of Kentucky Libraries], 2008. http://hdl.handle.net/10225/944.
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McNeill, Matthew Scott. "The Transient Receptor Potential Melastatin 7 is required for early melanophore survival and facets of both embryonic and larval motility in zebrafish." Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/1162.
Full textLiu, Ying [Verfasser]. "Regulation of transient receptor potential canonical channels TRPC3 and TRPC6 in kidney diseases / Ying Liu." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2013. http://d-nb.info/103109749X/34.
Full textShaifta, Yasin Mohammad. "Transient receptor potential channels (TRPC) in human cells : characterisation using over expression and knock-down." Thesis, King's College London (University of London), 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.429098.
Full textWalker, Rebecca L. "Functional and molecular characterization of TRP channels in smooth muscle /." abstract and full text PDF (UNR users only), 2002. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3068507.
Full textCai, Shiwei. "The transient receptor potential channel 1 (TRPC1) mediates calcium-regulated differentiation in oral gingival keratinocytes /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/6390.
Full textGrimm, Christian [Verfasser]. "Endolysosomal Cation Channels of the Transient Receptor Potential Superfamily : Physiology, Pharmacology, and Mouse Models / Christian Grimm." München : GRIN Verlag GmbH, 2014. http://d-nb.info/1067618155/34.
Full textClarke, Rebecca. "An in vitro model for the study of transient receptor potential channels on human sensory neurons." Thesis, Queen's University Belfast, 2015. https://pure.qub.ac.uk/portal/en/theses/an-in-vitro-model-for-the-study-of-transient-receptor-potential-channels-on-human-sensory-neurons(0030aff3-6cb4-43bb-9a40-3e5cbc24c4bc).html.
Full textTakahashi, Nobuaki. "TRP channels as sensors of cellular redox status." 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/131892.
Full textSolanki, Sumeet A. ""Novel Role of the Transient Receptor Potential Canonical 3 (TRPC3) channel in Macrophage Apoptosis: Implications in Atherosclerosis”." University of Toledo Health Science Campus / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=mco1492707181753622.
Full textStenger, Bernhard [Verfasser], and Harald [Akademischer Betreuer] Mückter. "Transient receptor potential A1 channels and their role in the cytotoxicity of sulfur mustard / Bernhard Stenger ; Betreuer: Harald Mückter." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/116394887X/34.
Full textShang, Ye. "Mechanisms Regulating Transient Receptor Potential Cation Channel A1 (TRPA1) and Their Roles in Nociception and Nociceptive Sensitization." eScholarship@UMMS, 2020. https://escholarship.umassmed.edu/gsbs_diss/1092.
Full textAnandarajan, Mugilan. "The expression and function of airway epithelial Transient Receptor Potential (TRP) channels in hypersensitive airways in children with respiratory problems." Thesis, Queen's University Belfast, 2017. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.725331.
Full textMertens, Charlotte Nora [Verfasser]. "Functional expression of thermo-sensitive transient receptor potential channels in cultivated human corneal endothelial cells (HCEC-12) / Charlotte Nora Mertens." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1075757304/34.
Full textChretien, Chloé. "Un nouvel acteur dans la détection hypothalamique du glucose : les canaux Transient Receptor Potential Canonical (TRPC)." Thesis, Dijon, 2015. http://www.theses.fr/2015DIJOS027/document.
Full textHyperglycemia is detected and integrated by the mediobasal hypothalamus (MBH) which, in turn, inhibits food intake and triggers insulin secretion. The MBH houses specialized glucose-sensitive (GS) neurons, which directly or indirectly modulate their electrical activity in response to changes in glucose level. In a first study, we hypothesized that indirect detection of glucose by MBH GS neurons involves the secretion of endozepine by astrocytes, a gliotransmitter known to inhibit food intake in response to hyperglycemia. The present work shows that endozepines selectively activate anorexigenic MBH pro-opiomelanotortine (POMC) neurons. In the second study, we show that the direct detection of increased glucose level involves hypothalamic glucose-excited (HGE) neurons. Using pharmacological and genetic approaches, we demonstrate that the redox-sensitive Transient Receptor Potential Canonical 3 et 4 (TRPC3/4) channels are involved in MBH HGE response to glucose in vitro and increased insulin secretion and decreased food intake in response to cerebral hyperglycemia in vivo. We also obtained evidences that MBH TRPC3 channel is a critical new player for energy homeostasis. This thesis work identifies two new mechanisms involved in hypothalamic detection of hyperglycemia: the first based on the involvement of TRPC3/4 channels in HGE neurons and the second highlighting the astroglial endozepines as a relay of the “glucose” signal to POMC neurons
Amaral, Michelle Dawn. "TRP-ing down a TRK a new role for transient receptor potential channels as novel mediators of brain-derived neurotrophic factor actions at both sides of the excitatory synapse /." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2008p/amaral.pdf.
Full textKhajavi, Noushafarin [Verfasser]. "Analysis of expression and function of thermo-sensitive transient receptor potential channels in cultivated human conjunctival epithelial and human uveal melanoma cells / Noushafarin Khajavi." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1075757541/34.
Full textVelez-Ortega, Alejandra C. "TRPA1 CHANNELS IN COCHLEAR SUPPORTING CELLS REGULATE HEARING SENSITIVITY AFTER NOISE EXPOSURE." UKnowledge, 2014. http://uknowledge.uky.edu/physiology_etds/20.
Full textKawasaki, Brian Takeshi. "Players in the regulation of calcium entry activation of the transient receptor potential channels by src tyrosine kinase and a distinct role for the IP3 receptor c-terminus in store- and receptor-operated calcium entry /." Diss., Restricted to subscribing institutions, 2005. http://proquest.umi.com/pqdweb?did=994232031&sid=9&Fmt=2&clientId=1564&RQT=309&VName=PQD.
Full textKim, Hong Geun. "The roles of transient receptor potential channels in thermostatic behavior, in thermal acclimation, and in tonic immobility in the red flour beetle, Tribolium castaneum (coleoptera: tenebrionidae)." Diss., Kansas State University, 2014. http://hdl.handle.net/2097/18216.
Full textDepartment of Entomology
David C. Margolies and Yoonseong Park
Organisms are capable of sensing environmental conditions through diverse mechanisms. Transient receptor potential channels (TRPs) are a cation channel family that has been found to function in diverse sensing mechanisms. In this dissertation, I identified the function of several TRPs in thermosensing and mechanosensing in the red flour beetle, Tribolium castaneum. Candidate TRPs were chosen based on homology to TRPs found and studied in Drosophila melanogaster. To identify the function of candidate TRPs in T. castaneum, I suppressed the expression of target genes by RNA interference technique and investigated the phenotype of each treated beetle. Temperature is a major limiting environmental factor for organisms. I tested the function of candidate TRPs in thermotaxis (behavior) and thermal acclimation (physiology). Using bioinformatics approaches, I identified three candidate TRPs – painless, pyrexia, and trpA1 – involved in high temperature sensing. To test thermotactic behavior, I investigated beetle movement on a temperature arena with two separate temperature zones. Thermal acclimation was tested by pre-exposing beetles to either 42 °C for 10 min. When treated with double stranded RNA of TRPA1 (dstrpA1), the thermotactic response of beetles at 39 and 42 °C was reduced when compared to control groups. With pre-exposure at 42 °C, survivorship of dstrpA1-treated beetles significantly increased after one minute exposure at 52 °C compared to beetles that were not pre-exposed. With dspainless treatment, beetles showed lower response to thermal acclimation and lower long-term survivorship. Beetles treated with dspyrexia showed lower recovery after heat treatment without pre-exposure at 42 °C. To identify the function of candidate TRPs in mechanosensing, I evaluated dsRNA treated beetles for survival, walking behavior, and tonic immobility. Treatment with dsnompC and dstrpA5 resulted in failure in eclosion, causing 93 % mortality in both treatments. Survivors in dsnompC showed defects in elytra sclerotization. In dsnanchung and dsinactive treatments, adults showed abnormal walking behavior and reduced walking speed that were likely caused by defects of mechanosensing in folding of the joint between the femur and tibia. For tonic immobility, beetles with dsnanchung, dsinactive, dswaterwitch and dsick2 (insect cytokine 2) treatments showed increased sensitivity to mechanical stimulation leading to tonic immobility.
Mergler, Stefan [Verfasser]. "Functional expression of temperature-sensitive transient receptor potential channels (TRPs) in cultured human corneal and conjunctival cells : Relevance in the pathophysiology of ocular surface diseases / Stefan Mergler." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1078505403/34.
Full textMassullo, Pam. "Aberrant subcellular targeting of the G185R neutrophil elastase mutant associated with severe congenital neutropenia induces premature apoptosis of differentiating promyelocytes & expression and function of the transient receptor potential 2 (TRPM2) ion channel in dendritic cells." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1172865905.
Full textChapleau, Christopher Allen. "The developmental functions of BDNF and MECP2 on dendritic and synaptic structure." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2008p/chapleau.pdf.
Full textDumesnil, Dennis. "Neurological Responses to a Glucose Diet in Caenorhabditis elegans." Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc1011786/.
Full textTheis, Thomas [Verfasser], and MELITTA [Akademischer Betreuer] SCHACHNER. "Functional roles of transient receptor potential canonical channels and myristoylated alanine-rich protein kinase C substrate as novel interaction partners of the neural cell adhesion molecule NCAM and polysialic acid in Mus musculus (Linnaeus, 1758) / Thomas Theis. Betreuer: Melitta Schachner." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2013. http://d-nb.info/1035503840/34.
Full textDantas, Bruna Priscilla Vasconcelos. "O carvacrol reduz a pressão arterial via ativação de canais receptores de potencial transiente em ratos espontaneamente hipertensos." Universidade Federal da Paraíba, 2014. http://tede.biblioteca.ufpb.br:8080/handle/tede/8055.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
TRP channels have been extensively studied in many physiological and pathological processes involved in blood pressure regulation. Carvacrol is well known to act on TRP channels in the vasculature, however there are no studies of its effects in hypertensive rats. Our aim was to evaluate the contribution of TRP channels in hypertension and evaluate the effects of carvacrol on TRP channels of SHR. In an electrophysiological approach, carvacrol (300 μM) inhibited the barium current, suggesting a reduction of calcium influx through L-type voltage-operated Ca2+ channels. We found that the mRNA expression of the following TRP channels: TRPV1 (p=0.0007), TRPV4 (p=0.0002), TRPM7 (p=0.0091) and TRPM8 (p=0.0008) are decreased and TRPC1 (p=0,02) are increased in SHR compared to control. In aortic rings preparations precontracted with 1 μM of phenylephrine, carvacrol (10-8 - 3x10-4 M) induced vasorelaxation in WKY (pD2 = 4.88 0.09, Emax = 100.73 2.24%, n = 6) and SHR (pD2 = 4.93 0.08, Emax= 110.06 2.07%, n = 6) in the presence of functional endothelium and that effect was not altered after endothelium removal in WKY (pD2 = 5.09 0.08, Emax = 99.60 0.88%, n = 6) and SHR (pD2 = 5.00 0.08, Emax = 101.23 1.96%, n = 6), proposing an endotheliumindependent mechanism. To assess the role of TRP channels, aortic rings were incubated with ruthenium red. In this assay, the vasorelaxant response was not changed in the WKY. On the other hand both potency (p<0.001) and efficacy (p<0.001) were reduced in SHR, suggesting that carvacrol could activate the subtypes TRPV in hypertensive animals. When using magnesium, equally potency (p<0.001) and pharmacological efficacy (p<0.01) were attenuated in both WKY and SHR, suggesting the involvement of TRPM7. In preparations with 2-APB, CPZ and BCTC, the vasorelaxant effect was potentiated (p<0.01) in both WKY and SHR, suggesting the participation of TRPV1, TRPM8 and TRPM7 channels in the vasorelaxant effect induced by carvacrol. Nevertheless, in the presence of capsaicin, the vasodilator effect was attenuated (p<0.001) in both WKY and SHR endorsing a possible action of carvacrol on TRPV1 and TRPV4 channel. In addition, in vivo studies showed that carvacrol produced hypotension and bradycardia in unanesthetized WKY and SHR. In order to address the cardiovascular responses in vivo, we performed experiments using ruthenium red and capsaicin to evaluate the contribution of TRP channels in this effect. Our results suggested an action of carvacrol on TRPV1 and TRPV4, confirming the in vitro assays. In conclusion, these results suggest that the expression of TRPV1, TRPV4, TRPM7 and TRPM8 was reduced and TRPC1 increased in SHR and carvacrol induced a vasorelaxant effect probably by acting on TRPV1, TRPV4, TRPC1, TRPM7 and TRPM8 in SHR. Furthermore, the in vivo effects induced by carvacrol exhibited a hypotensive and bradycardic activity and this effect, at least in part, is due to an activation of TRPV1 and TRPV4 channels in these responses.
Os canais TRP têm sido amplamente estudados, em diversos processos de regulação fisiológico e patológico no sistema cardiovascular. Carvacrol (5-isopropil-2metilfenol) é conhecido por agir na vasculatura ativando ou bloqueando canais TRP, entretanto não há relatos dos seus efeitos em ratos hipertensos. Nosso objetivo foi avaliar o envolvimento dos canais TRP na hipertensão e o papel do carvacrol nos efeitos cardiovasculares em ratos espontaneamente hipertensos. Em ensaios eletrofisiológicos carvacrol (300μM) promoveu inibição das correntes de bário, sugerindo uma inibição do influxo de cálcio por canais de Ca2+ tipo-L. Ao avaliar a expressão do RNAm dos canais TRP em SHR, observamos pela primeira vez que a expressão de TRPV1 (p=0,0007), TRPV4 (p=0,0002), TRPM7 (p=0,0091), TRPM8 (p=0,0008) foram diminuídas e TRPC1 (p=0,02) aumentada. Em anéis de aorta précontraídos com 1 μM de FEN, o carvacrol (10-8 - 3 ₓ 10-4 M) induziu vasorelaxamento em ratos wistar kyoto (WKY) (pD2 = 4,88 0,09, Emáx = 100,73 2,24%, n = 6; pD2 = 5,09 0,08, Emáx = 99,60 0,88%, n = 6) e em ratos espontaneamente hipertensos (SHR) (pD2 = 4,93 0,08, Emáx = 110,06 2,07%, n = 6) na presença e na ausência do endotélio funcional, respectivamente. Para avaliar a participação dos canais TRP, na ausência do endotélio funcional as preparações foram incubadas com vermelho de rutênio, em WKY não houve alteração da resposta, mas em animais SHR tanto sua potência (p<0,001) como sua eficácia (p<0,001) foram diminuídas, sugerindo que carvacrol pode estar agindo em TRPV nos SHR. Ao utilizar magnésio, em WKY e SHR tanto sua potência (p<0,01) quanto sua eficácia (p<0,001) farmacológica foram atenuadas, sugerindo ação sobre o canal TRPM7. Nas preparações com 2-APB, CPZ e BCTC os seus efeitos foram potencializados (p<0,01), sugerindo ação sobre os canais TRPV1, TRPC1, TRPM7 e TRPM8. Já com capsaicina, um ativador de TRPV1, esse efeito foi atenuado (p<0,001) confirmando uma possível ação do carvacrol sobre TRPV1. Nos estudos in vivo, com WKY e SHR não anestesiados, carvacrol produziu hipotensão e bradicardia, onde ao avaliar a ação dos canais TRP em ensaios com vermelho de rutênio e capsaicina pode-se sugerir uma possível ação de carvacrol sobre TRPV1 e TRPV4, diminuindo a pressão arterial, corroborando com os ensaios in vitro. Em conclusão, esses resultados sugerem que os canais TRPV1, TRPV4, TRPM8 e TRPM7 têm sua expressão diminuída e TRPC1 a expressão aumentada em animais SHR e carvacrol induz efeito vasorelaxante provavelmente agindo em TRPV1, TRPV4, TRPC1, TRPM7 e TRPM8 em SHR. Além disso, os efeitos induzidos por carvacrol in vivo mostraram uma atividade hipotensora e bradicárdica e uma possível influencia dos canais TRPV1 e TRPV4 nessas respostas.
Dantas, Bruna Priscilla Vasconcelos. "Participação dos canaisTRP nos efeitos cardiovasculares induzidos por carvacrol em ratos." Universidade Federal da Paraíba, 2010. http://tede.biblioteca.ufpb.br:8080/handle/tede/6854.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The pharmacological effects of carvacrol, a monoterpenoid phenol, on the cardiovascular system were studied in normotensive rats, using in vivo and in vitro techniques. In superior mesenteric artery rings isolated from rats with functional endothelium carvacrol (10-8 - 3 ₓ 10-4 M) concentration-dependently relaxed phenylephrine-induced contractions (pD2 = 4.59 0.02, MR = 103.03 1.5%, N=8) and this effect was not altered after removal of the endothelium (pD2 = 4.36 0.02, MR = 111.03 4.8%, N=8), suggesting that the vasorelaxant response induced by carvacrol appears to be independent of vascular endothelium. Furthermore, carvacrol antagonized the vasoconstriction induced by high K+ solution (Tyrode with 80 mM of KCl) (pD2 = 4.12 0.01, MR = 94.38 3.97%, N=6), inhibited contraction elicited by CaCl2 in depolarizing (KCL 60 mM) nominally without Ca2+ medium out carvacrol also antagonized the contractions induced by the L-type Ca2+ channel agonist, S(-)-Bay K 8644 (pD2 = 4.537 0.023, MR = 9.8 3.58%, N=6), indicating that the vasodilatation involve probably the inhibition of Ca2+ influx through L-type voltage-dependent calcium channels (Cav type-L). Additionally, carvacrol antagonized the contractions induced by CaCl2 in nominally without Ca2+ medium in the presence of PHE and nifedipine, suggesting a possible inhibition of calcium influx by store operated channels (SOC), receptor operated channels (ROC) and/or TRP channels. Interestingly, in a depolarizing (KCL 60 mM) nominally without Ca2+ medium and in the presence of nifedipine, carvacrol also inhibited the contraction induced by CaCl2, suggesting a probable inhibition of SOC and/or TRP channels. To evaluate the involvement of TRP channels in the vasorelaxant effect induced by carvacrol, non-selective inhibitors were used. No change in the relaxation response was observed in the presence of ruthenium red (pD2= 4.31 0.029, N=6), however, the effect induced by carvacrol was potentiated by La3+ (pD2 = 5.231 0,04, N=6), Gd3+ (pD2 = 4.97 0.02, N=6) or Ni2+ (pD2 = 5.079 0.02, N=6), furthermore, Mg2+ (pD2 = 4.168 0.021; MR = 81.12 4.03%, N=6) attenuated the relaxation elicited by carvacrol, suggesting that monoterpenoid may to action on TRPC1, TRPC3, TRPC6 and TRPM7 channels. Carvacrol also induced hypotension and bradycardia in non-anesthetized normotensive rats. In conclusion, these results suggest that carvacrol induced vasorelaxant effect in superior mesenteric artery rats isolated probably inhibiting Ca2+ influx by Cav, SOC (TRPC1), ROC (TRPC1 or TRPC6) and TRPM7 channels. Moreover, the effects induced by carvacrol in normotensive non-anesthetized rats showed a hypotensive and bradycardic activity.
Os efeitos farmacológicos de carvacrol, um fenol monoterpenóide, sobre o sistema cardiovascular, foi estudado em ratos normotensos, usando técnicas in vivo e in vitro. Carvacrol (10-8 - 3 ₓ 10-4 M) induziu vasorelaxamento dos anéis de artéria mesentérica superior isolada de rato pré-contraídos com 10 μM FEN (pD2 = 4,59 0,02, Emáx = 103,03 1,5%) na presença do endotélio funcional e esse efeito não foi alterado após a remoção do endotélio (pD2 = 4,36 0,02, Emáx = 111,03 4,8%), sugerindo, portanto, que a resposta vasorelaxante induzida por carvacrol parece ser independente do endotélio vascular. Interessantemente em anéis pré-contraídos com KCl 80 mM (pD2 = 4,12 0,01, Emáx = 94,38 3,97%), observou-se uma diminuição na sua potência e na sua eficácia farmacológica, sugerindo um passo comum na via que seria um aumento citosólico dos níveis de cálcio. Adicionalmente, carvacrol antagonizou, de maneira dependente de concentração, as contrações induzidas por CaCl2 em meio despolarizante nominalmente sem Ca2+ e induziu relaxamento das contrações induzidas pelo S(-)-Bay K 8644 (pD2 = 4,537 0,023, Emáx = 91,8 3,58%) com uma diminuição na sua eficácia farmacológica, sugerindo uma inibição do influxo de cálcio por canais de Ca2+ tipo-L. Além disso, antagonizou as contrações induzidas por CaCl2 em meio nominalmente sem cálcio, na presença de FEN e nifedipina, sugerindo uma provável inibição do influxo de cálcio por SOC, ROC e/ou canais TRP. Como também, em um meio despolarizante e nominalmente sem cálcio na presença de nifedipina esse mesmo antagonismo foi observado, ressaltando a provável inibição dos SOC e/ou canais TRP. Para avaliar a participação dos canais TRP, as preparações foram incubadas com La3+ (pD2 = 5,231 0,04) , Gd3+ (pD2 = 4,97 0,02) e Ni2+ (pD2 = 5,079 0,02) onde seu efeito foi potencializado sugerindo sua ação sobre os canais TRPC e ao utilizar magnésio (pD2 = 4,168 0,021 e Emáx = 81,12 4,03%) tanto sua potência quanto sua eficácia farmacológica foi atenuada, sugerindo inibição do canal TRPM7. Nos estudos in vivo, em ratos normotensos não anestesiados, carvacrol produziu hipotensão e bradicardia. Em conclusão, esses resultados sugerem que carvacrol induz efeito vasorelaxante em anéis de artéria mesentérica superior isolada de rato por inibir provavelmente TRPM7, como também inibir o influxo de cálcio por Cav, SOC, ROC e ou TRPC1 e 6. Além disso, os efeitos induzidos por carvacrol em ratos normotensos não anestesiados mostrou uma atividade hipotensora e bradicárdica.
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Full textDie Aktivierung und Aggregation von Thrombozyten sind zwei elementare Prozesse für das Abdichten verletzter Gefäßwände und damit zur Verhinderung von exzessivem Blutverlust. Unter pathologischen Bedingungen kann die Thrombozytenaggregation jedoch zur unkontrollierten Thrombusbildung und folglich zum irreversiblen Gefäßverschluss führen. Daher ist eine präzise Regulation der Thrombozytenaktivierung wichtig, um effizient Gefäßverletzungen zu schließen aber gleichzeitig eine unkontrollierte Thrombusbildung zu verhindern. Schnelle Veränderungen der zytoplasmatischen Konztentration von Kationen stellen ein Kernelement der Signaltransduktion während der Plättchenaktivierung dar. In dieser Arbeit wurden die Rolle von Ca2+ und Mg2+ Kanälen in der Regulation der Thrombozytenfunktion untersucht. Orai1, der bedeutendste store-operated calcium (SOC) Kanal in Thrombozyten, ist nicht nur entscheidend für verschiedene Signalwege, sondern reguliert möglicherweise auch receptor-operated calcium entry (ROCE). Die Kopplung zwischen dem Orai1-Signalkomplex und canonical transient receptor potential channel (TRPC) Isoformen wurde als entscheidender Schritt in der Aktivierung in der Aktivierung von store-operated calcium entry (SOCE) und ROCE in humanen Thrombozyten vermutet. Die Frage nach der funktionellen Relevanz der Interaktion zwischen Orai und TRPC Isoformen blieb jedoch unbeantwortet. Im ersten Teil dieser Arbeit wurde der funktionelle Crosstalk zwischen Orai1 und TRPC6 adressiert. Hierbei zeigte sich, das Orai1-vermittelter SOCE die Aktivität der Phosholipasen (PL) C und D steigert, die Diacylglycerol (DAG) Produktion verstärkt und schließlich TRPC6-vermittelten ROCE via DAG reguliert, was darauf hindeutet, dass die Regulation der TRPC6 Kanalaktivität unabhängig von einer direkten Interaktion mit Orai1 zu sein scheint. Darüber hinaus führte die Doppeldefizienz von Orai1 und TRPC6 zu verringerten Ca2+ Konzentrationen in intrazellulären Ca2+-Speichern und im Zytoplasma der Thrombozyten. Überraschenderweise war auch die ATP-Sekretion erhöht, was eventuell den Ca2+-Einstrom durch P2X1 verstärkt und möglicherweise das starke Ca2+-Defizit in den doppeldefizienten Thrombozyten kompensiert. Außerdem wurde gezeigt, dass Orai1 und TRPC6 nicht für die Aktivierung und Aggregation von Thrombozyten sowie für die Thrombusbildung mittlels G protein-gekoppelter Rezeptoren (GPCR) benötigt werden. Transient receptor potential melastatin-like 7 (TRPM7) enthält eine zytosolische Serin/Threonin-Kinase Domain. Bislang wurden zwar wenige in vitro Substrate der TRPM7 Kinase identifiziert, jedoch ist die physiologische Rolle dieser Kinase immer noch unbekannt. Im zweiten Teil dieser Arbeit wurden Mäuse mit einer Punktmutation, welche die katalytische Aktivität der TRPM7 Kinase blockiert (Trpm7KI) eingesetzt um die Rolle der TRPM7 Kinase für die Funktion von Thrombozyten zu untersuchen. In Trpm7KI Thrombozyten war der Metabolismus von phosphatidylinositol-4,5-bisphosphat (PIP2) und die Ca2+-Mobilisierung nach Aktivierung des Rezeptors Glykoprotein (GP) VI schwer beeinträchtigt, was darauf hindeutet, dass die Aktivität der TRPM7 Kinase die Funktion der PLC reguliert. Aus diesem Signaltransduktionsdefekt in Trpm7KI Thrombozyten resultierte eine verringerte Aggregatbildung unter Flussbedingungen und ein Schutz der Tiere vor arteriellen Thrombosen und ischämischem Schlaganfall. Zusammenfassend heben diese Ergebnisse die Kinasedomäne von TRPM7 als einen ausschlaggebenden Bestandteil in Signalkaskaden hervor und implizieren eine Rolle dieser Domäne in der Pathogenese von ischämischen Kardio- und zerebrovaskulären Erkrankungen