Dissertations / Theses on the topic 'Transfer free energy'
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Liu, Yang, and 刘洋. "Free energy simulations of important biochemical processes." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196036.
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Chemistry
Doctoral
Doctor of Philosophy
Richmond, Graham J. "Collision-induced energy transfer in ground and excited state free radicals." Thesis, Heriot-Watt University, 2006. http://hdl.handle.net/10399/2156.
Full textKaris, Klas. "The Free Energy of Vesicular Transmembrane Lipid Transfer Studied With Molecular Dynamics Simulations." Thesis, KTH, Teoretisk fysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152473.
Full textCrichton, Hilary Jane. "Spectroscopic probes of collisional energy transfer in ground and excited state free radicals." Thesis, Heriot-Watt University, 2004. http://hdl.handle.net/10399/342.
Full textAdrian, Marlene [Verfasser]. "Energy transfer in free-standing van der Waals heterostructures after optical excitation / Marlene Adrian." Kassel : Universitätsbibliothek Kassel, 2019. http://d-nb.info/1187165239/34.
Full textErmantraut, Andreas [Verfasser], Ingo [Akademischer Betreuer] Krossing, and Thorsten [Akademischer Betreuer] Koslowski. "The experimental determination of the Gibbs free energy of transfer of single Ions without sxtra‐thermodynamic assumptions." Freiburg : Universität, 2018. http://d-nb.info/1165503239/34.
Full textOkazaki, S., N. Yoshii, and K. Fujimoto. "Molecular dynamics study of free energy of transfer of alcohol and amine from water phase to the micelle by thermodynamic integration method." AIP Publishing, 2012. http://hdl.handle.net/2237/20838.
Full textOkazaki, S., N. Yoshii, and K. Fujimoto. "Molecular dynamics study of solubilization of immiscible solutes by a micelle: Free energy of transfer of alkanes from water to the micelle core by thermodynamic integration method." AIP Publishing, 2010. http://hdl.handle.net/2237/20837.
Full textÖhman, Amanda. "Towards a fossil free steel sector : Conditions for technology transfer of hydrogenbased iron and steel in Europe." Thesis, KTH, Skolan för industriell teknik och management (ITM), 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-264106.
Full textFör att nå målen uppsatta i Parisavtalet behöver energiintensiva industrier kraftigt minska sina utsläpp, däribland järn- och stålindustrin. Direktreduktion med fossilfri vätgas är en teknologi med potential att minska utsläppen från järn och ståltillverkning till praktiskt taget noll men kräver stora mängder fossilfri el. Detta examensarbete undersöker de energimässiga förutsättningarna för denna teknik i en europeisk kontext. Tre länder som producerar primärstål är utvalda som fokusländer i studien; Tyskland, Frankrike och Italien. Resultaten av studien visar att inget av de utvalda länderna i dagsläget har optimala sociotekniska förutsättningar för tekniken. Frankrike är det land med de bästa energimässiga förutsättningarna men saknar några viktiga faktorer för att vara en möjliggörande socioteknisk miljö. Tyskland å andra sidan har de mest lovande förutsättningarna för en lämplig socioteknisk miljö men står inför utmaningar när det kommer till energisystemet och tillgången på fossilfri el. För att skapa förutsättningar för denna teknik är det viktigt med koordinerade omställningar i energisektorn och industrin, policys som möjliggör dessa omställningar samt ett väl fungerande samarbete mellan industrin, akademin, beslutsfattare och andra viktiga aktörer. Studien visar också att de platser där nuvarande stålverk för primärstål finns inte har de bästa förutsättningar för förnybar elproduktion och att en vätgasbaserad process inte är optimal, baserat på den förnybara kapaciteten och de transmissionsbegränsningar som finns idag i elsystemet. Det finns istället möjlighet till decentraliserade värdekedjor, där varje process placeras där de mest lämpliga förhållandena finns. Detta kan exempelvis innebära att vätgas eller järnsvamp produceras där tillgången till fossilfri el är god, för att sedan transporteras till stålverken för de resterande processtegen.
Björk, Erik. "Energy Efficiency Improvements in Household Refrigeration Cooling Systems." Doctoral thesis, KTH, Tillämpad termodynamik och kylteknik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-93061.
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Ryberg, Per. "Concerted or Stepwise? : β-Elimination, Nucleophilic Substitution, Copper Catalysed Aziridination and Ruthenium Catalysed Transfer Hydrogenation Studied by Kinetic Isotope Effects and Linear Free-Energy Relationships." Doctoral thesis, Uppsala universitet, Avdelningen för organisk kemi, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2008.
Full textHan, Yunqing. "THEORETICAL STUDY OF THERMAL ANALYSIS KINETICS." UKnowledge, 2014. http://uknowledge.uky.edu/me_etds/35.
Full textZelleke, Theodros Gaschau Tena [Verfasser], Dominik [Gutachter] Marx, and Lars [Gutachter] Schäfer. "Computer simulations of methylamine dehydrogenase : free energy landscape and proton transfer pathways in oxidative deamination of methylamine / Theodros Gaschau Tena Zelleke ; Gutachter: Dominik Marx, Lars Schäfer ; Fakultät für Chemie und Biochemie." Bochum : Ruhr-Universität Bochum, 2018. http://d-nb.info/1161942068/34.
Full textZelleke, Theodros [Verfasser], Dominik [Gutachter] Marx, and Lars [Gutachter] Schäfer. "Computer simulations of methylamine dehydrogenase : free energy landscape and proton transfer pathways in oxidative deamination of methylamine / Theodros Gaschau Tena Zelleke ; Gutachter: Dominik Marx, Lars Schäfer ; Fakultät für Chemie und Biochemie." Bochum : Ruhr-Universität Bochum, 2018. http://d-nb.info/1161942068/34.
Full textHolmgren, Tomas. "Emissions of organic compounds from technosphere articles : Measurements and modeling of mass transfer from consumer goods and building materials to air and water." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-66363.
Full textHerrera, Andrés Medina. "Estrutura eletrônica da amino- e dimetilamino-benzonitrila em meio usando métodos híbridos de QM/MM." Universidade Federal de Goiás, 2015. http://repositorio.bc.ufg.br/tede/handle/tede/5123.
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In this research we studied the structural and electronic properties of the ground state of molecules amino-benzonitrile (ABN) and dimethylamino-benzonitrile (DMABN), isolated and in different solvents.We performed computer simulations of those molecules in different solvents as cyclohexane, dichloromethane, acetonitrile and water. The structure electronic method MP2 (second order perturbation Møller-Plesset) was used to perform quantum calculations. To study the molecules in solvent we used the hybrid sequential QM/MM method combined with the free energy gradient method. The dual fluorescence to this type of molecules is a process that has been much studied but it is not well clarified that is the cause of the process. We performed the optimization of the molecules in an isolated state and in different solvents to determine the ground state structure. In the case of the DMABN molecule the optimization was performed both at room temperature and at low temperature, near the melting point of the solvent. We studied minimum energy point and some transition states of this molecules associated with the pyramidalization or the rotation of the amino group. The results showed that the molecules are pyramidal when they are isolated, and that in polar solvent they became less pyramidal. The rotation of amino group is unfavored in both molecules, increasing this effect in polar solvents.
Neste trabalho foram estudadas as propriedades estruturais e eletrônicas do estado fundamental das moléculas amino-benzonitrila (ABN) e dimetilamino-benzonitrila (DMABN), isoladas e em diferentes meios solventes. Foram realizadas simulações computacionais das moléculas em diferentes meios como, ciclohexano, diclorometano, acetonitrila e água. O método de estrutura eletrônica MP2 (Møller-Plesset em segunda ordem de perturbação) foi usado para fazer os cálculos quânticos. Para o estudo das moléculas em meio foi utilizado o método hibrido QM/MM sequencial combinado com o método de gradiente de energia livre. A dupla fluorescência para este tipo de moléculas é um processo que tem sido bastante estudado, mas ainda não está bem esclarecido qual é o causador do processo. Foram realizadas as otimizações das moléculas em estado isolado e nos diferentes meios, para determinar a estrutura do estado fundamental. No caso da molécula de DMABN a otimização foi feita tanto em temperatura ambiente como em baixas temperaturas, próximas do ponto de fusão dos solventes. Foram estudados pontos de mínimo e alguns estados de transição dessas moléculas associados à piramidalização ou à rotação do grupo amino. Os resultados mostram que essas moléculas são piramidais quando isoladas, e que em meio polar elas se tornam menos piramidais. A rotação do grupo amino é desfavorável em ambas as moléculas, aumentando esse efeito em meios polares.
Blagoi, Gabriela. "Fluorescence resonance energy transfer (FRET) based sensors for bioanalysis." ScholarWorks@UNO, 2004. http://louisdl.louislibraries.org/u?/NOD,146.
Full textTitle from electronic submission form. "A dissertation ... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry."--Dissertation t.p. Vita. Includes bibliographical references.
Leveneur, Sébastien. "Catalytic synthesis and decomposition of peroxycarboxylic acids." Phd thesis, INSA de Rouen, 2009. http://tel.archives-ouvertes.fr/tel-00560883.
Full textVaccaro, Carlos. "Use of luminescence energy transfer probes to detect genetic variants." Thesis, University of North Texas, 2004. https://digital.library.unt.edu/ark:/67531/metadc4566/.
Full textBeck, Michael, Niko Hildebrandt, and Hans-Gerd Löhmannsröben. "Quantum dots as acceptors in FRET-assays containing serum." Universität Potsdam, 2006. http://opus.kobv.de/ubp/volltexte/2006/950/.
Full textWindsor, Kramer Michelle Anne. "Development of a FRET biosensor to detect the pathogen mycoplasma capricolum." Diss., Columbia, Mo. : University of Missouri-Columbia, 2005. http://hdl.handle.net/10355/4289.
Full textThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (January 11, 2006) Includes bibliographical references.
Li, Xuelian. "USING CONJUGATED POLYMERS AS BIOLOGICAL SENSOR BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER." OpenSIUC, 2011. https://opensiuc.lib.siu.edu/dissertations/321.
Full textBalloi, Eleonora. "Investigating conformational changes of proteins using Förster Resonance Energy Transfer." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/investigating-conformational-changes-of-proteins-using-frster-resonance-energy-transfer(fd5f53e7-9464-40bf-9c87-6fbd0a3d8804).html.
Full textMerckx, R., Thomas Swift, R. Rees, Guyse J. F. R. Van, E. Schoolaert, Clerck K. De, H. Thienpont, and V. V. Jerca. "Förster resonance energy transfer in fluorophore labeled poly(2-ethyl-2-oxazoline)s†." Royal Society of Chemistry, 2020. http://hdl.handle.net/10454/18374.
Full textDye-functionalized polymers have been extensively studied to understand polymer chain dynamics, intra or inter-molecular association and conformational changes as well as in practical applications such as signal amplification in diagnostic tests and light-harvesting antennas. In this work, the Förster resonance energy transfer (FRET) of dye-functionalized poly(2-ethyl-2-oxazoline) (PEtOx) was studied to evaluate the effect of dye positioning and polymer chain length on the FRET efficiency. Therefore, both α (initiating terminus)- or ω (terminal chain end)-fluorophore single labeled and dual α,ω-fluorescent dye labeled PEtOx were prepared via cationic ring opening polymerization (CROP) using 1-(bromomethyl)pyrene as the initiator and/or 1-pyrenebutyric acid or coumarin 343 as the terminating agent, yielding well-defined PEtOx with high labeling efficiency (over 91%). Fluorescence studies revealed that intramolecular FRET is most efficient for heterotelechelic PEtOx containing both pyrene and coumarin 343 fluorophores as chain ends, as expected. A strong dependence of the energy transfer on the chain length was found for these dual labeled polymers. The polymers were tested in both dilute organic (chloroform) and aqueous media revealing a higher FRET efficiency in water due to the enhanced emissive properties of pyrene. The application of dual labeled polymers as fluorescent probes for temperature sensing was demonstrated based on the lower critical solution temperature behavior of the PEtOx. Furthermore, these polymers could be successfully processed into fibers and thin films. Importantly, the fluorescence properties were retained in the solid state without decreasing the FRET efficiency, thus opening future possibilities for application of these materials in solar cells and/or sensors.
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Dennis, Allison Marie. "Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer pairs." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37079.
Full textMartin, Sarah Friede. "Fluorescence resonance energy transfer studies of protein interactions." Thesis, St Andrews, 2008. http://hdl.handle.net/10023/537.
Full textChen, Chi. "Lanthanide Energy Transfer Donors on Nanoparticles Surfaces : From Fundamental Mechanisms to Multiplexed Biosensing." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS196/document.
Full textOptical multiplexing based on nanoparticles provides many advantages for multiparameter biosensing and imaging. However, the changes in one parameter also lead to changing of other parameters, and thus, color, lifetime, or intensity could not be used as an independent parameter, respectively. This thesis can be divided into two aspects. The first one focuses on developing time-resolved single-nanoparticle multiplexing based on Förster resonance energy transfer (FRET) from lanthanide complexes to quantum dot (QD) to fluorescent dyes. Systematical investigation of all different combinations with a broad range of numbers of donors and acceptors on QD are presented, and the experimental results are compared with theoretical modelling. The result do not only contribute to a full understanding of such complicated multi donor-acceptor energy transfer pathways on nanoparticles but also open the opportunity to use the models for developing new strategies to achieve the QD with independent tunable color, lifetime and intensity. The second aspect focuses on the energy transfer mechanism from Tb to gold nanoparticle (AuNP). Nanosurface energy transfer (NSET) proved to be an operational mechanism in PL quenching by AuNPs, which is important information for the development, characterization, and application of nanobiosensors based on PL quenching by AuNPs
Guo, Jiajia. "Time-resolved Multiplexed Förster Resonance Energy Transfer for Nucleic Acid Biosensing." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS162/document.
Full textNucleic acid biomarkers, which involve in gene expression control, are found specific for many kinds of cancers. Förster Resonance Energy Transfer (FRET) based applications are one of the most promising for nucleic acid biosensing. As parallel detection of multiple nucleic acids is highly demanded and spectral multiplexing is limited by optical crosstalk, temporal multiplexing is used for opening another dimension of the multiplexing. The thesis focuses on developing different Tb-to-dye FRET distances to create specific intensity signals corresponding to different nucleic acid sequences. The Tb-dye distances can be tuned by specific location of the Tb donor using different lengths of DNA. Amplification technologies, such as hybridization chain reaction (HCR) and rolling circle amplification (RCA), are used to achieve simplicity, rapidity, selectivity, and sensitivity of nucleic acid detection. Temporal multiplexing FRET was also combined with spectral (color) multiplexing for higher order multiplexed detection. Moreover, a single Tb-QD FRET modeling demonstrated the possibility of nanoparticle-based temporal multiplexing
Wu, Yu-Tang. "Förster Resonance Energy Transfer Immunoassays Using Engineered Proteins for Breast Cancer Biomarker Detection." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS340/document.
Full textEngineered affinity proteins have raised great interest due to their extremely small size compared to full length antibodies. Such small binding proteins have demonstrated many advantages such as quick biodistribution, good penetration into tumor tissue, and fast elimination from serum and nondiseased tissues. Thus, they are expected to be excellent alternatives to antibodies for clinical applications. This thesis focuses on the development of biosensors based on engineered antibodies and time-resolved Förster resonance energy transfer (FRET) through biological recognition of biomarkers. FRET-based immunoassays are established using terbium complexes (Tb) as FRET donors and semiconductor quantum dots (QDs) as FRET acceptors. The exceptional photophysical properties of the Tb-QD FRET pair allow for ultrasensitive quantitative biosensing. Single-domain antibodies (sdAb) and small engineered scaffold antibodies (ADAPT) are used to investigate different antibody-conjugation strategies for quantifying human epidermal growth factor receptors (EGFR, HER2) as clinical biomarkers. This work can be considered as a prerequisite to implementing QDs into applied clinical diagnostics
Linden, Stina. "Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA112094.
Full textThis thesis investigates the use of time-gated FRET microscopy for detection of colocalization of two membrane proteins, E- and N-cadherin. These proteins are important for cell-cell contacts and have an important role in the epithelial to mesenchymal transition (EMT), a key process in cancer metastasis. In EMT cells lose their epithelial markers (such as E-cadherin) and gain mesenchymal markers (such as N-cadherin), increasing their motility and invasiveness, enabling escape from the primary tumor into the bloodstream as so called circulating tumor cells (CTCs). This manuscript focuses on the detection of CTCs that have undergone partial EMT, displaying a hybrid phenotype (epithelial-mesenchymal) and co-express E- and N-cadherin, by FRET co-localization studies on a model cell line. FRET (Förster resonance energy transfer) is a non-radiative energy transfer between two molecules that are in resonance and in close proximity (ca. 1-20 nm). A co-localization of E- and N-cadherin in clusters would therefore be detectable by FRET. The staining of the cadherins was done by using specific antibodies labelled with a long lifetime donor, the terbium complex Lumi4-Tb (TbL4) from Lumiphore, Inc., and various acceptors. The long lifetime donor and long lifetime sensitized acceptor emission (FRET) could be imaged in a time-gated microscopy setup. Time -gated imaging has several advantages compared to steady state imaging in terms of efficient background suppression in biological samples. The setup described in this manuscript is based on the use of an intensified CCD camera, a pulsed UV-laser excitation source, and a defined (µs) delay between excitation and image acquisition. In addition to the E- and N-cadherin FRET experiments the time-gated FRET imaging microscopy was used to investigate different biological samples (intracellular and membrane located). Although both protein markers could be successfully imaged on the same cells, FRET between E- and N-cadherin or E- and E-cadherin could not be detected. Control experiments with antibodies against the same primary antibody revealed strong time-gated FRET signals due to binding of both donor and acceptor antibodies to the same primary antibodies. The successful time-gated imaging of two different antibodies separated by a few nanometers demonstrates the feasibility of probing protein-protein interaction and co-localization at membranes using TbL4 based time-gated FRET imaging. Microsecond time-gated imaging is especially interesting for the investigation of protein-protein interactions in highly autofluorescent biological samples such as cancer tissues
Thomson, Cameron Ian. "Probing the Nature of Cellulosic Fibre Interfaces with Fluorescence Resonance Energy Transfer." Diss., Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/16277.
Full textKalinin, Stanislav. "Electronic Energy Transfer within Asymmetric Pairs of Fluorophores: Partial Donor-Donor Energy Migration (PDDEM)." Doctoral thesis, Umeå : Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-338.
Full textWren, David Andrew. "Measuring calcium in Pseudomonas aeruginosa using a genetically encoded fluorescence resonance energy transfer (FRET) sensor." Connect to online resource, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1460883.
Full textDurach, Maxim. "Giant Plasmonic Energy and Momentum Transfer on the Nanoscale." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/phy_astr_diss/42.
Full textHildebrandt, Niko. "Lanthanides and quantum dots : time-resolved laser spectroscopy of biochemical Förster Resonance Energy Transfer (FRET) systems." Phd thesis, Universität Potsdam, 2006. http://opus.kobv.de/ubp/volltexte/2007/1268/.
Full textFörster Resonanzenergietransfer (FRET) spielt eine wichtige Rolle in biochemischen Anwendungen, wie z.B. DNA-Sequenzierung, intrazellulären Protein-Protein-Wechselwirkungen, molekularen Bindungsstudien, in-vitro-Diagnostik und vielen anderen. Zur quantitativen und qualitativen Analyse werden FRET Systeme normalerweise durch molekulare Erkennung von Biomolekülen, die mit Donator- und Acceptorluminophoren markiert sind, ermöglicht. Durch die besonderen photophysikalischen Eigenschaften sowohl von Lanthanidkomplexen (Ln-Komplexen), als auch Halbleiternanokristallen (sog. Quantenpunkten oder Quantumdots - QD), sind diese besonders für FRET Anwendungen geeignet. In der vorliegenden Arbeit wird effizienter FRET zwischen Ln-Komplexen und QD in biochemischen Systemen demonstriert. Die notwendigen theoretischen und praktischen Grundlagen über FRET, Ln-Komplexe, QD und die verwendeten biochemischen Modelle werden dargestellt, und wissenschaftliche als auch kommerzielle Anwendungen werden präsentiert. FRET kann zur Messung von strukturellen Veränderungen und Dynamiken im Bereich von ca. 1 bis 10 nm verwendet werden. Der sehr starke und gut charakterisierte Bindungsprozess zwischen Streptavidin (Strep) und Biotin (Biot) wird als biomolekulares Modellsystem eingesetzt. Ein FRET System wird durch Streptavidinkonjugation mit Ln-Komplexen und QD-Biotinylierung etabliert. Drei Ln-Komplexe (einer mit Tb3+ und zwei mit Eu3+ als Zentralion) werden als Donatoren verwendet, und neben QD werden zwei weitere Acceptoren, das lumineszierende, quervernetzte Protein Allophycocyanin (APC) und ein kommerzieller Fluoreszenzfarbstoff (DY633), untersucht. FRET kann für alle Donator-Acceptor Paare nachgewiesen werden, zum einen durch sensibilisierte Acceptorlumineszenz und zum anderen durch eine über 1000-fach erhöhte Lumineszenzabklingzeit der QD mit über 100 Mikrosekunden. Mittels detailierter photophysikalischer Charakterisierung der Donatoren und Acceptoren können die Biokonjugate analysiert und die FRET Parameter berechnet werden. Für die QD FRET Systeme ergeben sich extrem große Försterradien von über 100 Å, die wesentlich größer sind als für APC und DY633 (ca. 80 bzw. 60 Å). Besondere Aufmerksamkeit gilt der Wechselwirkung mit den Zusatzreagenzien Boratpuffer, Bovines Serumalbumin (BSA), Natriumazid und Kaliumfluorid (KF) in den wässrigen Lösungen. Im Vergleich zum ausgiebig charakterisierten und vielfach verwendeten Donator-Acceptor Paar aus Europium-tris(Bipyridin) (Eu-TBP) und APC wird eine mehr als 10-fache Senkung der Nachweisgrenze für das FRET-System, bestehend aus Tb-Komplex und QD, erreicht. In azidfreiem Boratpuffer (pH 8,3) mit 2 % BSA und 0,5 M KF wird eine subpicomolare QD-Nachweisgrenze für dieses System aufgezeigt. Um den Transfer des Strep-Biot Modellsystems in eine echte in-vitro-diagnostische Anwendung zu demonstrieren, werden zwei Immuntests zum HCG-(Humanes Choriongonadotropin)-Nachweis untersucht. Sowohl HCG als auch monoklonale anti-HCG Maus-IgG-(Immunoglobulin G)-Antikörper werden mit dem Tb-Komplex bzw. mit QD markiert. Obwohl kein ausreichender Nachweis für FRET in einem immunometrischen Assay (oder Sandwichassay) erbracht werden kann, wird FRET in einem direkten HCG-IgG Assay erzielt, wodurch die Realisierbarkeit von Ln-QD Donator-Acceptor Paaren zur hochsensitiven Anwendung in der in-vitro-Diagnostik gezeigt werden kann.
Xu, Jingyue. "Sensitive and mutiplexed microRNA quantification using amplified time-gated Förster resonance energy transfer." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASS137.
Full textAs new generation of biomarkers, microRNAs are associated with many cancers and diseases, which has led to a great demand for developing clinical miRNA diagnostic methods. Isothermal amplification technologies, such as rolling circle amplification and catalytic hairpin assembly, have emerged as powerful methods for highly rapid, specific and sensitive microRNA assays. This thesis focuses on developing microRNA biosensors based on isothermal amplification technologies and time-resolved Förster resonance energy transfer from lanthanide complexes to organic dyes or quantum dots. The proposed amplified microRNA biosensors have very low limits of detections, and are applied to human clinical samples, successfully revealing the relevance for cancer diagnostics. As simultaneous detection of multiple microRNAs is highly demanded, temporal multiplexed detection of microRNAs is also realized based on distinguishable excited-state lifetimes of Tb complexes and dyes. Moreover, the amplified microRNA nanosensor based on Tb-to-quantum dots FRET demonstrated the possibility of spectral multiplexed detection of microRNAs with high sensitivity and selectivity
Herrmann, Janning [Verfasser], Volker [Gutachter] Deckert, and Michael [Gutachter] Börsch. "Plasmon-Controlled resonant energy transfer : influence of optical antennas on the energy transfer rate and efficiency of single molecule FRET-Pairs / Janning Herrmann ; Gutachter: Volker Deckert, Michael Börsch." Jena : Friedrich-Schiller-Universität Jena, 2019. http://d-nb.info/1207273163/34.
Full textHalpern, Micah. "DEVELOPMENT AND FORENSIC APPLICATION OF DYE PROBE FLUORESCENCE RESONANCE ENERGY TRANSFER FOR IMPROVED DETECTION OF CHANGES IN DN." Master's thesis, University of Central Florida, 2008. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2871.
Full textM.S.
Department of Chemistry
Sciences
Forensic Science MS
Altevogt, Andrea [Verfasser], and Willi [Akademischer Betreuer] Bannwarth. "Synthese und Anwendung neuartiger Förster-Resonanz-Energie-Transfer (FRET) Systeme in DNA." Freiburg : Universität, 2011. http://d-nb.info/112346376X/34.
Full textHildebrandt, Niko. "Lanthanides and quantum dots time-resolved laser spectroscopy of biochemical Förster resonance energy transfer (FRET) systems /." [S.l.] : [s.n.], 2006. http://opus.kobv.de/ubp/volltexte/2007/1268.
Full textOlejko, Lydia [Verfasser], and Ilko [Akademischer Betreuer] Bald. "Förster resonance energy transfer (FRET)-based nanophotonics using DNA origami structures / Lydia Olejko ; Betreuer: Ilko Bald." Potsdam : Universität Potsdam, 2017. http://d-nb.info/1218402261/34.
Full textPasupulleti, Venkata Kiran. "Characterization of viral proteases from Norwalk virus, poliovirus, and transmissible gastroenteritis virus using a fluorescence resonance energy transfer assay." Thesis, Kansas State University, 2012. http://hdl.handle.net/2097/15068.
Full textDepartment of Diagnostic Medicine/Pathobiology
Kyeong-Ok Chang
Positive sense RNA viruses include diverse groups of viruses that cause a wide variety of diseases in humans and animals. Most of these viruses encode proteases that cleave the viral polyprotein into intermediate or mature functional proteins during virus replication. As these proteases play a critical role in virus replication, they represent an attractive target for the development of antiviral drugs. In this study, the main goal was to establish assay systems and characterize the enzymatic activity of related proteases from Norwalk virus (NV), poliovirus, and transmissible gastroenteritis virus (TGEV). These proteases share several common characteristics including a typical chymotrypsin-like fold, a Cys residue as a nucleophile in the catalytic triad (or dyad) composed of Cys, His and Glu (or Asp) residues, and a preference for a Glu or Gln residue at the P1 position on the substrate. We cloned and expressed proteases from these viruses and characterized their enzymatic activities using a fluorescence resonance energy transfer (FRET) assay using a specific FRET substrate corresponding to each viral protease. First, assay conditions of the FRET assay was optimized for each virus protease. Second, inhibition profiles of each virus protein were investigated using five commercially available standard protease inhibitors (chymostatin, leupeptin, antipain, TPCK, and TLCK). The inhibition studies showed that TPCK inhibited NV, poliovirus, and TGEV proteases with varying strength, and chymostatin inhibited only NV protease. All other inhibitors had little effects on the virus proteases. The established FRET assays should facilitate screening potential antivirals.
Sahoo, Dhananjaya [Verfasser]. "Synthesis of Molecular Rulers to Study Distance and Orientation Dependent Förster Resonance Energy Transfer (FRET) / Dhananjaya Sahoo." Bielefeld : Universitätsbibliothek Bielefeld, 2019. http://d-nb.info/1196640580/34.
Full textMitchell, Amanda. "Development of a Novel Genetically Encoded FRET System Using the Unnatural Amino Acid Anap." Thesis, Boston College, 2016. http://hdl.handle.net/2345/bc-ir:107177.
Full textFörster Resonance Energy Transfer (FRET) offers a powerful approach to study biomolecular dynamics in vitro as well as in vivo. The ability to apply FRET imaging to proteins in living cells provides an excellent tool to monitor important dynamic events such as protein conformational changes, protein-protein interactions, and proteolysis reactions. However, selectively incorporating two distinct fluorophores into the target protein(s) that are capable of FRET interaction within the complex cellular milieu is challenging. Consequently, terminal fusion to genetically encoded fluorescent proteins has emerged as the predominant labeling strategy for FRET studies in vivo. However, a major limitation of this strategy stems from the large size of the fluorescent proteins, which may perturb the native properties of the target, and restricted attachment only to the termini of the target. We reasoned that using genetically encoded fluorescent unnatural amino acids would overcome several of these challenges associated with currently available labeling strategies owing to their small size and the ability to introduce them site- specifically and co-translationally. Here, we report the use of the fluorescent unnatural amino acid “Anap” as a FRET donor with green and yellow fluorescent protein acceptors. We demonstrate the utility of this labeling strategy using proteolysis and conformational change models, and step towards in vivo studies by further developing a proteolysis system in cell lysates
Thesis (MS) — Boston College, 2016
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Ellis, Jonathan. "FRET analysis of splicing factors involved in exon and intron definition in living cells." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/4397.
Full textClerté, Caroline. "Structure et dynamique globales de la protéine U1A humaine étudiées par spectroscopie de fluorescence." Paris 6, 2001. http://www.theses.fr/2001PA066405.
Full textDogra, Navneet. "INVESTIGATING PROTEIN - BILAYER COMPLEXES: A STUDY OF LIGAND - RECEPTOR INTERACTIONS AT MODEL MEMBRANE SURFACE BY USING ELECTRONIC ABSORPTION SPECTROSCOPY AND FLUORESCENCE RESONANCE ENERGY TRANSFER." OpenSIUC, 2014. https://opensiuc.lib.siu.edu/dissertations/812.
Full textSarca, Anamaria Daniela. "FRET-based detection and quantification of HIV-1 Virion Maturation." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263567.
Full text京都大学
新制・課程博士
博士(医学)
甲第23106号
医博第4733号
京都大学大学院医学研究科医学専攻
(主査)教授 小柳 義夫, 教授 松田 道行, 教授 朝長 啓造
学位規則第4条第1項該当
Doctor of Medical Science
Kyoto University
DFAM
Schuler, Benjamin, Everett A. Lipman, Peter J. Steinbach, Michael Kumke, and William A. Eaton. "Polyproline and the "spectroscopic ruler" revisited with single-molecule fluorescence." Universität Potsdam, 2005. http://opus.kobv.de/ubp/volltexte/2007/1222/.
Full textWegner, David Karl. "Förster Resonance Energy Transfer from Terbium Complexes to Quantum Dots for Multiplexed Homogeneous Immunoassays and Molecular Rulers." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA112109/document.
Full textFörster resonance energy transfer (FRET) is a non-radiative energy transfer from a donor to an acceptor in close proximity. Due to its extremely sensitive distance dependence in the 1 – 20 nm range, FRET plays an important role in nanobiotechnology. Thereby FRET can be used as signal transduction system but also for the distance estimation between donor and acceptor. The selected FRET acceptors in this work were semiconductor nanocrystals (quantum dots, QDs). This type of luminophore is well known for its superior photophysical properties. Their strong and broad absorption and their bright, narrow-band, and size-tunable photoluminescence (PL) emission make QDs ideally suited for FRET application. Combing QDs as FRET acceptors with luminescent terbium complexes (LTC) as FRET donors offers exceptionally large Förster distances of more than 10 nm. The Förster distance is characteristic of a FRET pair and is the distance at which the FRET efficiency equals 50 %. A large Förster distance is desirable as it offers the detection of biological interactions over large distances. LTC are suitable FRET donors for QDs because they provide long excited-state lifetimes in the millisecond range. This long PL decay time enables time-gated measurements for the suppression of autofluorescence and PL of directly excited QDs, which strongly increases the detection sensitivity. Additionally, the structured PL emission bands of LTCs together with the size-tunable PL emission bands of QDs make this FRET pair ideal for the application in multiplexed diagnostics, which is the measurement of multiple biomarkers in a single sample.The PhD thesis consists of two parts. In the first part the LTC-QD FRET pair was used within homogeneous FRET immunoassays for the detection of the biomarkers prostate specific antigen (TPSA), neuron-specific enolase (NSE), carcinoembryonic antigen (CEA), and epidermal growth factor receptor (EGFR). The immunoassay sensitivity was optimized using different types of antibodies IgG, F(ab’)2,F(ab), and for EGFR single heavy chain antibodies, which differ largely in their size. The use of small-volume serum samples and measurements on clinical as well customized fluorescence plate readers result in picomolar detection limits for all measured biomarkers. In addition to these QD-based in vitro diagnostic tests, a detailed study of the different FRET-systems using time-resolved spectroscopy was performed. The investigation revealed the influence of the different antibodies on distance, functionality, and sensitivity of the FRET immunoassays. The study was completed by the measurement of NSE and CEA in a duplexed format and real patient samples were investigated.The second part was to use FRET for nanometric distance measurements as molecular or spectroscopic ruler. Time-resolved FRET measurements enabled the calculation of the distance between donor and acceptor. Therefore two different binding strategies were investigated to establish a close proximity between the LTC-donor to the QD-acceptor, namely biotin-streptavidin recognition and polyhistidine mediated self-assembly. A detailed time-resolved study was performed of QDs with different sizes, shapes, and surface coatings in combination with LTC bound to three different host biomolecules, which also possessed different sizes, shapes, orientations, and binding conditions. The analysis of the multi-exponential decay curves of donor and acceptor allowed to obtain information about the size, shape, and biofunctionality of the investigated QD bioconjugates. The results were in agreement with other structural analysis methods, such as transmission electron microscopy (TEM) or dynamic light scattering (DLS), but with the advantage of a homogeneous measurement with three-dimensional resolution (not possible for TEM), without the inclusion of a hydration shell (drawback for DLS), and at low concentration in the same environment as used for the biological application