Relevant bibliographies by topics / Transfection

Academic literature on the topic 'Transfection'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 July 2024

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Journal articles on the topic "Transfection"

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Toth, Maria, Manuel Reithofer, Gregory Dutra, Patricia Pereira Aguilar, Astrid Dürauer, and Reingard Grabherr. "Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells." Viruses 16, no. 3 (March 10, 2024): 426. http://dx.doi.org/10.3390/v16030426.

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(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or on laborious generation of stable cell lines. Alternatively, BacMam, a transduction process using the baculovirus, can be employed. (2) Six transfecting agents were compared to baculovirus transduction in terms of transient and stable protein expression characteristics of the model protein ACE2-eGFP using HEK293-6E, CHO-K1, and Vero cell lines. Furthermore, process optimization such as expression enhancement using sodium butyrate and TSA or baculovirus purification was assessed. (3) Baculovirus transduction efficiency was superior to all transfection agents for all cell lines. Transduced protein expression was moderate, but an 18-fold expression increase was achieved using the enhancer sodium butyrate. Ultracentrifugation of baculovirus from a 3.5 L bioreactor significantly improved the transduction efficiency and protein expression. Stable cell lines were obtained with each baculovirus transduction, yet stable cell line generation after transfection was highly unreliable. (4) This study demonstrated the superiority of the BacMam platform to standard transfections. The baculovirus efficiently transduced an array of cell lines both transiently and stably and achieved the highest efficiency for all tested cell lines. The feasibility of the scale-up of baculovirus production was demonstrated and the possibility of baculovirus purification was successfully explored.
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Cheow, Pheik-Sheen, Tiong Kit Tan, Adelene Ai-Lian Song, Khatijah Yusoff, and Suet Lin Chia. "An improved method for the rescue of recombinant Newcastle disease virus." BioTechniques 68, no. 2 (February 2020): 96–100. http://dx.doi.org/10.2144/btn-2019-0110.

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Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method.
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Zhang, Jianxiong, Yawei Hu, Xiaoqing Wang, Peng Liu, and Xiaofang Chen. "High-Throughput Platform for Efficient Chemical Transfection, Virus Packaging, and Transduction." Micromachines 10, no. 6 (June 10, 2019): 387. http://dx.doi.org/10.3390/mi10060387.

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Intracellular gene delivery is normally required to study gene functions. A versatile platform able to perform both chemical transfection and viral transduction to achieve efficient gene modification in most cell types is needed. Here we demonstrated that high throughput chemical transfection, virus packaging, and transduction can be conducted efficiently on our previously developed superhydrophobic microwell array chip (SMAR-chip). A total of 169 chemical transfections were successfully performed on the chip in physically separated microwells through a few simple steps, contributing to the convenience of DNA delivery and media change on the SMAR-chip. Efficiencies comparable to the traditional transfection in multi-well plates (~65%) were achieved while the manual operations were largely reduced. Two transfection procedures, the dry method amenable for the long term storage of the transfection material and the wet method for higher efficiencies were developed. Multiple transfections in a scheduled manner were performed to further increase the transfection efficiencies or deliver multiple genes at different time points. In addition, high throughput virus packaging integrated with target cell transduction were also proved which resulted in a transgene expression efficiency of >70% in NIH 3T3 cells. In summary, the SMAR-chip based high throughput gene delivery is efficient and versatile, which can be used for large scale genetic modifications in a variety of cell types.
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Gam, Jeremy J., Breanna DiAndreth, Ross D. Jones, Jin Huh, and Ron Weiss. "A ‘poly-transfection’ method for rapid, one-pot characterization and optimization of genetic systems." Nucleic Acids Research 47, no. 18 (August 2, 2019): e106-e106. http://dx.doi.org/10.1093/nar/gkz623.

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Abstract Biological research is relying on increasingly complex genetic systems and circuits to perform sophisticated operations in living cells. Performing these operations often requires simultaneous delivery of many genes, and optimizing the stoichiometry of these genes can yield drastic improvements in performance. However, sufficiently sampling the large design space of gene expression stoichiometries in mammalian cells using current methods is cumbersome, complex, or expensive. We present a ‘poly-transfection’ method as a simple yet high-throughput alternative that enables comprehensive evaluation of genetic systems in a single, readily-prepared transfection sample. Each cell in a poly-transfection represents an independent measurement at a distinct gene expression stoichiometry, fully leveraging the single-cell nature of transfection experiments. We first benchmark poly-transfection against co-transfection, showing that titration curves for commonly-used regulators agree between the two methods. We then use poly-transfections to efficiently generate new insights, for example in CRISPRa and synthetic miRNA systems. Finally, we use poly-transfection to rapidly engineer a difficult-to-optimize miRNA-based cell classifier for discriminating cancerous cells. One-pot evaluation enabled by poly-transfection accelerates and simplifies the design of genetic systems, providing a new high-information strategy for interrogating biology.
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Gardiner, Donald L., Tina S. Skinner-Adams, Tobias Spielmann, and Katharine R. Trenholme. "Malaria transfection and transfection vectors." Trends in Parasitology 19, no. 9 (September 2003): 381–83. http://dx.doi.org/10.1016/s1471-4922(03)00187-9.

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Peng, Zhiqiang, Jianping Xiong, Hanzhi Dong, and Wuping Li. "Preparation of Polymer Nanocarrier Material and Its Application in B-Cell Lymphoma." Science of Advanced Materials 12, no. 10 (October 1, 2020): 1524–34. http://dx.doi.org/10.1166/sam.2020.3877.

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The β-cyclodextrin (β-CD) is coupled with polyethyleneimine (PEI 600 Da) to produce a polymer nanocarrier material (PyD-W) with good biocompatibility and high transfection rate. First, the test was performed to study the influence of different factors on the transfection efficiency of PyD-W materials in terms of cell type and transfection system. Then the effect of adding wheat germ agglutinin on the material-cell membrane binding when transfecting cells by PyD-W materials was studied. The influence of temperature and cell phagocytosis inhibitors on the entire transfection process were taken into consideration at the same time. In the end, the escape ability of PyD-W material carrying plasmid DNA in lysosomes was studied. After analyzing the performance of PyD-W material transfected cells, the appropriate transfection staining conditions was selected to be applied in the study of the inhibitory properties of mouse B-cell lymphoma cells. In the experiment, the results showed that the cell type had a great influence on the material. The N/P ratio should not be set too high. Prolong the transfecting and fostering time appropriately could increase the transfection efficiency. The addition of wheat germ lectin would significantly reduce the enrichment ability on the cell surface of plasmid DNA carried by PyD-W. Increasing the temperature could also increase the transfection efficiency of PyD-W materials, cell phagocytosis inhibitors would not have a significant impact on transfection, and the accumulation of PyD-W materials in lysosomes would be gradually released from lysosomes with time going by. According to the above results, PyD-W carrying plasmid DNA was transfected into mouse B lymphoma cells and normal B cells using similar transfection methods. The results show that B lymphoma cells (38B9) corresponded significant mRNA levels is lower than the mRNA level of normal B cells (P < 0.05). It is detected by cell count and CCK-8 kit that the growth of cells in the group overexpressing PyD-W is significantly inhibited (P < 0.01).
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Yin, Jingwen, and Henry R. Henney Jr. "Stable transfection ofAcanthamoeba." Canadian Journal of Microbiology 43, no. 3 (March 1, 1997): 239–44. http://dx.doi.org/10.1139/m97-033.

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The promoter activity of an Acanthamoeba polyubiquitin gene was analyzed in its homologous system. A modified calcium phosphate transfection method using a neomycin marker vector was developed to achieve highly efficient transfection of the Acanthamoeba polyubiquitin gene into Acanthamoeba cells. In this transfection procedure, the calcium phosphate – DNA complex was formed gradually in the medium during incubation with cells and precipitated on the cells. The crucial factors for obtaining efficient transfection were the pH (6.95) of the transfection buffer used for the calcium phosphate precipitation and the amount (25 μg/96-well tissue culture plate) and form (circular) of transfecting DNA. Under these conditions, Acanthamoeba isolate 1B6 was transfected at an efficiency of about 40% with the constructed vector pOPSBU, a pOP13CAT-based polyubiquitin gene incorporated neomycin resistance vector. Acanthamoeba polyphaga was transfected at an efficiency of about 10% with this vector. Transfection of both Acanthamoeba strains appeared to result in low copy plasmid integration (about two copies per cell are suggested). The chloramphenicol acetyltransferase (CAT) assays showed that the promoter of the Acanthamoeba polyubiquitin gene in the constructed vector was especially strong in A. polyphaga, thus the pOPSBU – Acanthamoeba system may be useful for the construction of cDNA expression libraries, as well as for the expression of cloned genes.Key words: Acanthamoeba, transfection, ubiquitin, promoter, vector, CAT assay.
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Nie, Leng, Li Zeng Gao, Xi Yun Yan, and Tai Hong Wang. "Functionalized Tetrapod-Like ZnO Nanostructrures for DNA Gene Delivery." Solid State Phenomena 121-123 (March 2007): 747–50. http://dx.doi.org/10.4028/www.scientific.net/ssp.121-123.747.

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Amino-modified tetrapod-like ZnO nanostructures were tried as novel carriers for mammalian cell transfections. The nanostructures consisted of four needle-shaped tetrahedrally arranged legs connected at the center. After silica coating and amino modification, ZnO nanostructures complexes bound plasmid DNA through electrostatic interactions in aqueous solution. When mixed with cells, DNA-nanostructures attached easily onto cell membranes and entered the cells for gene expressions. Due to high positive charge densities on surfaces and needle-shaped tetrahedral structures, functionalized ZnO used as carriers for cell transfections with both high transfection efficiency and little cytotoxicity. And a possible transfection machamism was proposed in this report.
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Chung, Namjin, Louis Locco, Kevin W. Huff, Steven Bartz, Peter S. Linsley, Marc Ferrer, and Berta Strulovici. "An Efficient and Fully Automated High-Throughput Transfection Method for Genome-Scale siRNA Screens." Journal of Biomolecular Screening 13, no. 2 (January 23, 2008): 142–48. http://dx.doi.org/10.1177/1087057107312032.

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RNA interference (RNAi), combined with the availability of genome sequences, provides an unprecedented opportunity for the massive and parallel investigations of gene function. Small interfering RNA (siRNA) represents a popular and quick approach of RNAi for in vitro loss-of-function genetic screens. Efficient transfection of siRNA is critical for unambiguous interpretation of screen results and thus overall success of any siRNA screen. A high-throughput, lipid-based transfection method for siRNA was developed that can process eighty 384-well microplates in triplicate (for a total of 30,720 unique transfections) in 8 h. Transfection throughput was limited only by the speed of robotics, whereas the cost of screening was reduced. As a proof of principle, a genome-scale screen with a library of 22,108 siRNAs was performed to identify the genes sensitizing cells to mitomycin C at concentrations of 0, 20, and 60 nM. Transfection efficiency, performances of control siRNAs, and other quality metrics were monitored and demonstrated that the new, optimized transfection protocol produced high-quality results throughout the screen. ( Journal of Biomolecular Screening 2008:142-148)
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Chong, Kevin Wai Yin, Alan Yiu-Wah Lee, Evelyn S. C. Koay, Sze Jee Seet, and Nam Sang Cheung. "pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin." Journal of Neuroscience Methods 158, no. 1 (November 2006): 56–63. http://dx.doi.org/10.1016/j.jneumeth.2006.05.017.

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Dissertations / Theses on the topic "Transfection"

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Ma, Nan. "Tailoring optical fibers for cell transfection." Thesis, University of St Andrews, 2012. http://hdl.handle.net/10023/3177.

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Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane. Of the different optical light sources that have been used, femtosecond laser based transfection has been one of the most effective methods for optical transfection which is generally implemented using a free-space bulk optical setup. Here in this thesis, a few novel fabrication methods are devised to obtain easy and inexpensive fabrication of microlensed optical fibers, which can be used to replace traditional optical setup and perform femtosecond optical transfection. These fabrication methods offer the flexibility to fabricate a microlens which can focus femtosecond laser pulses at 800 nm to a small focal spot whilst keeping a relatively large working distance. In conventional optical transfection methods the foreign genetic material to be transfected is homogenously mixed in the medium. This thesis reports the first realization of an integrated optical transfection system which can achieve transfection along with localized drug delivery by combining lensed fiber based optical transfection system with a micro-capillary based microfluidic system. Finally, based on an imaging fiber (coherent optical fiber bundle), the first endoscope-like integrated system for optical transfection with subcellular resolution epifluorescence imaging was built. The transfection efficiency of these fiber based systems is comparable to that of a standard free-space transfection system. Also the use of integrated system for localized gene delivery resulted in a reduction of the required amount of genetic material for transfection. The miniaturized, integrated design opens a range of exciting experimental possibilities, such as the dosing of tissue slices to study neuron activities, targeted drug delivery, and in particular for using endoscope-like integrated systems for targeted in vivo optical microsurgery.
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Finlay, Siân. "Modulation of macrophage phenotype using adenoviral transfection." Thesis, University of Aberdeen, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409252.

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The initial aim of this study was to examine the nature of the interaction between adenovirus and transfected macrophage, and to characterise the molecular mechanisms underlying macrophage response to adenoviral infection. Results showed that adenoviral transfection activated macrophages, promoting production of pro-inflammatory mediators and priming an enhanced response to other inflammatory stimuli.  Activation was dependent on the nuclear factor kappa B (NFkB) signalling pathway, which was activated within two hours of transfection, and could be prevented using an inhibitory adenovirus which blocked NFkB signalling.  The ultimate phenotype of the transfected macrophage was determined both by non-specific viral activation and by the properties of the transgene expressed. The second aim of this research was to investigate the effect of the local cytokine milieu on transgene expression in vitro.  Results showed that transgene expression under the control of two different promoter constructs was subject to regulation by pro-inflammatory mediators, by mechanisms at least partly dependent on the NFkB signalling pathway.  the results have important implications for the use of these promoters in gene therapy applications where the gene of interest is delivered into an inflammatory environment. The final stage of the project focused on the use of transfected macrophages in vivo, in a rat model of glomerulonephritis.  Results showed that transfected macrophages expressing the anti-inflammatory cytokine IL-4 localised with enhanced efficiency to inflamed glomeruli after intra-renal artery injection of the mechanisms for this were examined.  Better understanding of the mechanisms which promote localisation may ultimately permit the design of genetically-modified macrophages which selectively target sites of injury for delivery of anti-inflammatory cytokines.
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Horefti, Ioulia-Christina. "Aeroporation : a new method for cell transfection." Thesis, University of Essex, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364549.

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Yingyongnarongkul, Boon-ek. "Transfection agents : from traditional to miniaturised screening." Thesis, University of Southampton, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289566.

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Tsampoula, Xanthi. "Femtosecond cellular transfection using novel laser beam geometries." Thesis, St Andrews, 2009. http://hdl.handle.net/10023/909.

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Mthunzi, Patience. "Optical sorting and photo-transfection of mammalian cells." Thesis, University of St Andrews, 2010. http://hdl.handle.net/10023/1254.

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Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic channels and the cytoplasmic delivery of both genetic and non-genetic matter of varying sizes into mammalian cells. In this thesis several new findings specifically in the optical cell sorting as well as in the photo-transfection study fields are presented. In my optical cell sorting and guiding investigations, a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally engulfing polymer microspheres from their environment, is introduced. I explore how these intracellular dielectric tags influence the scattering and gradient forces upon these cells from an externally applied optical field. I show that intracellular polymer microspheres can serve as highly directional optical scatterers and that the scattering force can enable sorting through axial guiding onto laminin coated glass coverslips upon which the selected cells adhere. Following this, I report on transient photo-transfection of mammalian cells including neuroblastomas (rat/mouse and human), embryonic kidney, Chinese hamster ovary as well as pluripotent stem cells using a tightly focused titanium sapphire femtosecond pulsed laser beam spot. These investigations permitted advanced biological studies in femtosecond laser transfection: firstly, the influence of cell passage number on the transfection efficiency; secondly, the possibility to enhance the transfection efficiency via whole culture treatments of cells thereby, synchronizing them at the mitotic (M phase) as well as the synthesis phases (S phase) of the cell cycle; thirdly, this methodology can activate the up-regulation of the protective heat shock protein 70 (hsp70). Finally, I show that this novel technology can also be used to transfect mouse embryonic stem (mES) cell colonies and the ability of differentiating these cells into the extraembryonic endoderm.
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Lemoine, Jérôme. "Transfection de l'épithélium respiratoire nasal normal de souris." Reims, 2005. http://www.theses.fr/2005REIMP205.

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La @mucoviscidose est une maladie génétique affectant principalement les poumons, qui est due à la mutation du gène CFTR. La protéine CFTR est un canal chlorique. Le transfert du gène CFTR sauvage dans l'épithélium bronchique peut être obtenu à l'aide de vecteurs de gènes non-viraux. Ces transferts n'ont pas permis, jusqu'à présent, de corriger complètement le transport de chlorure. Dans cette étude, nous avons démontré que de l'ADN nu dissout dans de l'eau déminéralisée transfecte les cellules épithéliales nasales plus efficacement que dans une solution isotonique ou hypertonique. Cette méthode a été baptisée : transfection par choc hypotonique. Le plasmide serait internalisé par les cellules épithéliales au cours de l'étape dite de diminution régulée du volume cellulaire du choc hypotonique. La stratégie la plus simple pour améliorer la transfection a consisté à raccourcir le plasmide, en le coupant dans la portion bactérienne à l'aide d'enzymes de restriction. L'expression du transgène obtenue suite à l'administration de fragments d'ADN nu, dissous dans de l'eau pure, a atteint un très haut niveau. L'amplificateur transcriptionnel IE1 du CMV contient des sites de fixation pour NF-κB et ATF-CREB, qui ont été remplacés par des sites Oct-1. Les sites de fixation non-consensuels NF1 et Sp1, ont été remplacés par leurs homologues consensuels. Ces modifications ont permis de réduire la diminution de l'expression du transgène entre les jours un et dix. La thérapie génique de la mucoviscidose deviendra concevable s'il est possible d'atteindre et de maintenir pendant plusieurs semaines, un niveau élevé d'expression du transgène dans l'épithélium bronchique des patients
@Cystic fibrosis is a recessive genetic disease mostly affecting the lung. It is due to the mutation or the loss of the CFTR gene. The CFTR protein is a chloride channel located in the apical membrane of airway epithelial cells. CFTR gene transfer to the bronchial epithelium might be carried out by nonviral vectors, which appear insufficiently efficient to date. In this study, we have demonstrated that naked DNA dissolved in deionized water transfect the nasal airway epithelial cells 100-fold more efficiently than in an isotonic or hypertonic solution. This method has been named transfection by hypotonic shock. The plasmid DNA would be endocytosed by the airway epithelial cells during the so-called phase of regulatory volume decrease of the hypotonic shock. The simplest strategy to further improve gene transfer consisted in shortening the plasmid DNA by deleting a part of its backbone by using some restriction enzymes. The transgene expression achieved by some naked DNA fragments dissolved in pure water was over 21-fold higher than that obtained by the intact plasmid DNA. The CMV IE1 enhancer contains a few NF-κB and ATF-CREB binding sites, which were replaced by Oct-1 binding sites. Also, the non-consensual NF1 and Sp1 binding sites were replaced by their consensual sequences. These modifications reduced the decline of the transgene expression 3. 4-fold between day 1 and day 10. Gene therapy will become a conceivable therapeutic approach, if a high level of transgene expression is sustained for several weeks in the bronchial epithelium of cystic fibrosis patients
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Le, Bolc'h Gwénaelle. "Synthèse de phosphonolipides pour la transfection non-virale." Brest, 1997. http://www.theses.fr/1997BRES2032.

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La therapie genique apparait, de plus en plus, comme une solution envisageable pour vaincre les maladies hereditaires comme la mucoviscidose par exemple. L'objectif de ce travail est la synthese de composes pouvant favoriser la transfection d'un gene correcteur a travers les membranes cellulaires. Le premier chapitre bibliographique fait le point sur la therapie genique et sur les differents vecteurs non-viraux deja connus. Les deux chapitres suivants sont consacres a la synthese de composes susceptibles d'etre utilises comme vecteurs non-viraux de transfection. Les premieres evaluations de la capacite de transfection y sont egalement presentees. Puis nous ferons le bilan de ce travail et evoquerons les perspectives qui en resultent.
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Lemoine, Jérôme Desoize Bernard. "Transfection de l'épithélium respiratoire nasal normal de souris." S.l. : S.n, 2005. http://www.univ-reims.fr/BU//exl-doc/GED00000194.pdf.

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DUGONNET, DURAND FREDERIQUE. "Differentes methodes de transfection appliquees en therapie genique." Strasbourg 1, 1994. http://www.theses.fr/1994STR15097.

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Books on the topic "Transfection"

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Bielke, Wolfgang, and Christoph Erbacher, eds. Nucleic Acid Transfection. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-16430-9.

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Christoph, Erbacher, and SpringerLink (Online service), eds. Nucleic Acid Transfection. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2010.

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Ruth, Laura. Transfection and gene transfer: Technologies and markets. New York: Kalorama Information, 2002.

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Yanan, Yue. How Free Cationic Polymer Chains Promote Gene Transfection. Heidelberg: Springer International Publishing, 2013. http://dx.doi.org/10.1007/978-3-319-00336-8.

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Zarytova, V. F. Polyamine-containing DNA fragments. New York: Nova Science Publishers, 2011.

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Zarytova, V. F. Polyamine-containing DNA fragments. New York: Nova Science Publishers, 2011.

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Jassal, Ramesh Kumar. Remodelling recombinant glycoproteins made in CHO cells by transfection of glycosyltransferases. Leicester: De Montfort University, 1999.

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Wong, Michael J. Transfection and expression of human factor I cDNA in mammalian cells. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1993.

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Moyle, Katherine. La transfection de fibroblastes normaux par des ADN de papillomavirus humains. Sudbury, Ont: Université Laurentienne, 1992.

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Lasserre, Chantal. Hybridation cellulaire et transfection: Étude du maintien et de l'expression du matériel génétique introduit. Grenoble: A.N.R.T. Université Pierre Mendès France Grenoble 2, 1986.

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Book chapters on the topic "Transfection"

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Mehlhorn, Heinz. "Transfection." In Encyclopedia of Parasitology, 2786. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-43978-4_4415.

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Mehlhorn, Heinz. "Transfection." In Encyclopedia of Parasitology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-27769-6_4415-1.

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Feril, Loreto B. "Ultrasound-Mediated Gene Transfection." In Gene Therapy of Cancer, 179–94. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-561-9_10.

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Fischer, Wiebke, Marcelo Calderón, and Rainer Haag. "Hyperbranched Polyamines for Transfection." In Topics in Current Chemistry, 95–129. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/128_2010_64.

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Franklin, Roger, and Julian E. Sale. "Transient transfection of DT40." In Subcellular Biochemistry, 379–82. Dordrecht: Springer Netherlands, 2006. http://dx.doi.org/10.1007/978-1-4020-4896-8_29.

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Turrin, Cédric-Olivier, and Anne-Marie Caminade. "Dendrimers as Transfection Agents." In Dendrimers, 413–35. Chichester, UK: John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9781119976530.ch17.

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González, Blanca. "Ceramics for Gene Transfection." In Bio-Ceramics with Clinical Applications, 383–419. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118406748.ch13.

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Girard, Philippe, M. Derouazi, G. Baumgartner, M. Bourgeois, Martin Jordan, and Florian M. Wurm. "100 Liter Transient Transfection." In Animal Cell Technology: From Target to Market, 37–44. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0369-8_10.

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Bøe, Sigurd Leinæs, and Eivind Hovig. "Light-Induced mRNA Transfection." In Methods in Molecular Biology, 89–100. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-260-5_6.

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Rug, Melanie, and Alexander G. Maier. "Transfection of Plasmodium falciparum." In Methods in Molecular Biology, 75–98. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-026-7_6.

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Conference papers on the topic "Transfection"

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Tsong, Tian Y., and Ting Dong Xie. "Mechanisms of cell transfection by electroporation: Field induced DNA uptake and transfection efficiency." In 1992 14th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1992. http://dx.doi.org/10.1109/iembs.1992.5760978.

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Palanker, D., T. Chalberg, A. Vankov, P. Huie, F. E. Molnar, A. Butterwick, M. Calos, M. Marmor, and M. S. Blumenkranz. "Plasma-mediated transfection of RPE." In Biomedical Optics 2006, edited by Fabrice Manns, Per G. Söderberg, and Arthur Ho. SPIE, 2006. http://dx.doi.org/10.1117/12.649624.

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Stevenson, David, Ben Agate, Lynn Paterson, Tanya Lake, Muriel Comrie, Tom Brown, Andrew Riches, et al. "Optical transfection of mammalian cells." In Photonics Europe, edited by Romualda Grzymala and Olivier Haeberle. SPIE, 2006. http://dx.doi.org/10.1117/12.662325.

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Breunig, Hans Georg, Ana Batista, Aisada König, and Karsten König. "Towards laser-assisted microfluidic-cell transfection." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, edited by Daniel L. Farkas, James F. Leary, and Attila Tarnok. SPIE, 2019. http://dx.doi.org/10.1117/12.2509442.

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James, Molly B., and Todd D. Giorgio. "Nuclear-Associated Plasmid, But Not Cell-Associated Plasmid, is Correlated With Transgene Expression in Cultured Mammalian Cells." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2232.

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Abstract Intracellular plasmid is rapidly incorporated into the nucleus of HeLa cells following cationic lipoplex transfection. CV1 cells are less effective in translocating plasmid to the nucleus and also express less transgene than HeLa cells. Cultured HeLa and CV1 cells and corresponding isolated nuclei were analyzed after transfection of a Cy3 labeled pGreenLantern plasmid (Cy3-pGL). Flow cytometry was used to measure both plasmid delivery and transgene expression from the plasmid encoding a CMV promoter driven green fluorescent protein. During transfection, HeLa cells rapidly incorporated the plasmid, reaching a maximum of 80% Cy3-pGL positive cells 8 hours post-transfection. The average Cy3-pGL positive HeLa cell contained approximately 2470 plasmid copies. 48% of the nuclei isolated from the transfected HeLa cells were positive for the plasmid marker after 8 hours. In contrast to HeLa cells, fewer CV1 cells and CV1 nuclei incorporated plasmid DNA with peak transfection occurring after 12 hours for 36% of the cells and after 8 hours for 12% of the nuclei. However, the average Cy3-pGL positive CV1 cell did not have a significantly different number of total cellular plasmid copies than the average positive HeLa cell. CV1 nuclei, however, had half as much plasmid as HeLa nuclei. HeLa cells are more efficient than CV1 cells at transporting plasmid from the cytoplasm to the nucleus. This study demonstrates the use of a novel quantitative method to study plasmid transport from the cytoplasm to nucleus and the effect on transgene expression.
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Otsuka, Risa, Mitsuhiro Terakawa, Shunichi Sato, Yasushi Satoh, Kunio Takishima, Hiroshi Ashida, and Minoru Obara. "Laser-induced stress wave-assisted gene transfection: improved transfection efficiency with cationic liposome-modified plasmid DNA." In Biomedical Optics (BiOS) 2007, edited by Steven L. Jacques and William P. Roach. SPIE, 2007. http://dx.doi.org/10.1117/12.701734.

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Zhu, Qingfu, Ziyu Zhu, and Mei He. "3D Additive Manufacturing and Micro-Assembly for Transfection of 3D-Cultured Cells and Tissues." In ASME 2018 13th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/msec2018-6567.

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3D additive manufacturing, namely 3D printing, has been increasingly needed in the fabrication of biological materials and devices. Compared to traditional fabrication, direct 3D digital transformation simplifies the manufacturing process and enhances capability in geometric fabrication. In this paper, we demonstrated a rapid and low-cost 3D printing approach for “lego” assembly of micro-structured parts as an electro-transfection device. Electro-transfection is an essential equipment for engineering and regulating cell biological functions. Nevertheless, existing platforms are mainly employed to monolayer cell suspensions in vitro, which showed more failures for translating into tissues and in vivo systems constituted by 3D cells. The knowledge regarding the three-dimensional electric transport and distribution in a tissue microenvironment is lacking. In order to bridge the gap, we assembled PDMS parts molded from 3D-printed molds as the 3D-cell culture chamber, which connects arrays of perfusion channels and electrodes. Such design allows spatial and temporal control of electric field uniformly across a large volume of 3D cells (105∼106 cells). Most importantly, multi-dimensional electric frequency scanning creates local oscillation, which can enhance mass transport and electroporation for improving transfection efficiency. The COMSOL electrostatic simulation was employed for proof of concept of 3D electric field distribution and transport in this “lego” assembled electro-transfection device, which builds the foundation for engineering 3D-cultured cells and tissues.
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Lin, Yu-Cheng, Chung-Min Jen, Ming-Yuan Huang, and Xi-Zhang Lin. "Flow-type electroporation chips for gene transfection." In Micromachining and Microfabrication, edited by Carlos H. Mastrangelo and Holger Becker. SPIE, 2000. http://dx.doi.org/10.1117/12.395660.

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Jingcai Ma, Xiaona Cao, Shumin Zhou, Fanqiang Kong, Yuping Ren, Ronglan Zhao, Dongchun Liang, and Jingyu Zhang. "Phosphorylatable short peptide for facilitating DNA transfection." In 2009 International Conference on Future BioMedical Information Engineering (FBIE). IEEE, 2009. http://dx.doi.org/10.1109/fbie.2009.5405819.

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Domach, M. M., S. A. Khan, and A. A. Ataai. "Unleashed DNA production for transfection, vaccines, or labeling." In 2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC). IEEE, 2015. http://dx.doi.org/10.1109/nebec.2015.7117038.

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Reports on the topic "Transfection"

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Finch, Allison, Andrew Schaefer, Ephrath Tesfaye, John Trasatti, Julia Emmenecker, Nic Preyat, Ulrike Strauss, and Veda Sample. A proposal to align release standards for transfection reagents. BioPhorum, January 2024. http://dx.doi.org/10.46220/2023cgt015.

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McCormick, J. J. Malignant transformation of diploid human fibroblasts by transfection of oncogenes. Office of Scientific and Technical Information (OSTI), January 1992. http://dx.doi.org/10.2172/6852538.

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Kenney, James J. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes,. Fort Belvoir, VA: Defense Technical Information Center, July 1992. http://dx.doi.org/10.21236/ada253974.

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Kenny, James J. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes. Fort Belvoir, VA: Defense Technical Information Center, January 1991. http://dx.doi.org/10.21236/ada243424.

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Kenny, James J. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes. Fort Belvoir, VA: Defense Technical Information Center, February 1992. http://dx.doi.org/10.21236/ada245750.

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Kennedy, C. H., K. D. Kenyon, and J. Tesfaigzi. Transfection of normal human bronchial epithelial cells with the bcl-2 oncogene. Office of Scientific and Technical Information (OSTI), December 1995. http://dx.doi.org/10.2172/381812.

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Radu, Daniela Rodica. Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection. Office of Scientific and Technical Information (OSTI), January 2004. http://dx.doi.org/10.2172/837277.

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McCormick, J., and V. Maher. Malignant transformation of diploid human fibroblasts by transfection of oncogenes: Progress report, July 1986--June 1989. Office of Scientific and Technical Information (OSTI), January 1989. http://dx.doi.org/10.2172/6067022.

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DeMartini, James C., Abraham Yaniv, Jonathan O. Carlson, Arnona Gazit, Leonard E. Pearson, Kalman Perk, J. K. Young, Noam Safran, and A. Friedman. Evaluation of Naked Proviral DNA as a Vaccine for Ovine Lentivirus Infection. United States Department of Agriculture, September 1994. http://dx.doi.org/10.32747/1994.7570553.bard.

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Ovine lentivirus (OvLV) infection is widespread in sheep of the United States and Israel and is responsible for substantial economic losses. The primary goal of this project was to evaluate naked proviral DNA as a vaccine to induce protective immunity in sheep in endemic areas. Contrary to expectations, inoculation of sheep with proviral DNA derived from the full length OvLV molecular clone pkv72 did not result in detectable OvLV infection, but infectious virus was recovered from transfected ovine cells. Kv72 virus produced by these cells infected sheep and induced antibody responses, and was used as a viral challenge in subsequent experiments. To improve in vivo transfection efficiency and compare the viral LTR with other romoters, expression of reporter genes was studied in sheep transfected in vivo by injection of cationic liposome-DNA complexes; one formulation produced gene expression in a sheep for 4 months following a single intravenous injection. Since the pol-deleted OvLV construct was not stable in vivo, twelve lambs were injected with plasmids containing the Kv72 gag region (pCMVgag) or env region (pCMVenv), or saline. Prior to challenge, no detectable anti-OvLV immune responses were detected. Following homologous challenge with OvLV. Although the naked DNA approach to vaccination holds promise for control of ovine lentivirus-induced disease, further work needs to be done to develop more effective methods of transfecting sheep with DNA.
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McCormick, J. J. Malignant transformation of diploid human fibroblasts by transfection of oncogenes. Part 2, Progress report, July 1989--June 1992. Office of Scientific and Technical Information (OSTI), December 1992. http://dx.doi.org/10.2172/10107606.

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