Academic literature on the topic 'Transcriptomique spatiale'
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Journal articles on the topic "Transcriptomique spatiale"
Parreau, S., E. Molina, S. Dumonteil, R. Goulabchand, T. Naves, M. Bois, A. Fauchais, et al. "Étude transcriptomique spatiale d’artère temporale : nouvelles informations sur le processus de l’artérite à cellules géantes." La Revue de Médecine Interne 44 (June 2023): A89—A90. http://dx.doi.org/10.1016/j.revmed.2023.04.302.
Full textMaitre, Marlène, Thomas Daubon, and Frédéric Martins. "La transcriptomique spatiale de coupes histologiques : une technique de dernière génération pour le diagnostic médical." Innovations & Thérapeutiques en Oncologie 10, no. 6 (January 1, 2024): 1–11. http://dx.doi.org/10.1684/ito.2024.414.
Full textLequain, H., D. Cyril, N. Streichenberger, S. Thomas, L. Schaeffer, P. Leblanc, and P. Sève. "Approche par technique de transcriptomique spatiale pour élucider l’impact des granulomes sur le tissu musculaire dans les myosites sarcoïdosiques." La Revue de Médecine Interne 43 (December 2022): A383. http://dx.doi.org/10.1016/j.revmed.2022.10.096.
Full textPorte, Baptiste, Christophe Lepriol, and Andrée Delahaye. "Utilisation d’une approche de transcriptomique spatiale pour étudier les mécanismes de l’épileptogenèse dans un modèle d’épilepsie du lobe temporal chez le rat." Morphologie 106, no. 354 (September 2022): S1. http://dx.doi.org/10.1016/j.morpho.2022.06.053.
Full textDal Cin, J., A. Mahoudeau, M. Bensalah, L. Muraine, B. Tendrel, C. Anquetil, D. Amelin, et al. "Étude transcriptomique spatiale musculaire des myopathies nécrosantes auto-immunes : rôle de l’interféron induit par la nécrose dans le développement des séquelles fibro-adipeuses." La Revue de Médecine Interne 44 (December 2023): A373. http://dx.doi.org/10.1016/j.revmed.2023.10.079.
Full textMalartre, S., J. Dal Cin, C. Anquetil, D. Seilhan, F. Letournel, A. Madelaine, S. Léonard-Louis, Y. Allenbach, and O. Benveniste. "Étude du phénotype de la réponse musculaire au cours des syndrome des anti-synthétases par transcriptomique spatiale : implication de l’IFN-II et des macrophages dans la survie des lymphocytes B." La Revue de Médecine Interne 44 (December 2023): A373—A374. http://dx.doi.org/10.1016/j.revmed.2023.10.080.
Full textDissertations / Theses on the topic "Transcriptomique spatiale"
Parreau, Simon. "Etude du remodelage vasculaire de l’Artérite à Cellules Géantes par caractérisation inflammatoire et lésionnelle spatiale." Electronic Thesis or Diss., Limoges, 2024. http://www.theses.fr/2024LIMO0040.
Full textRational: Giant cell arteritis (GCA) is a vasculitis affecting the aorta and its main branches, characterized by an inflammatory infiltration and vascular remodeling. Ischemic complications of the disease are linked to intimal hyperplasia due to proliferation of myofibroblasts. Adventitial fibroblasts have been little studied in GCA and may play a role in vascular remodeling.Objectives: We hypothesize that adventitial fibroblasts are involved in GCA vascular remodeling.Methods: We included patients who had undergone temporal artery biopsy (TAB) for suspected GCA. We first studied in situ distribution of fibroblasts on temporal artery sections and their activation markers by immunohistochemistry. Using adventitial fibroblasts isolated from GCA patients and controls, functional tests on these fibroblasts were done. We performed a spatial transcriptomic study of temporal arteries from healthy subjects and GCA patients, using the Digital Spatial Profiler (Nanostring) to identify genomic expression signatures in the different arterial layers.Results: Adventitial fibroblasts from GCA patients express activation markers. They display proliferation, migration and secretion capacities. Enrichment analysis showed that functions related to inflammation/immune response and vascular remodeling were significantly restricted to the inner layers (intima and media). Activated immune functions included macrophage differentiation, T and B lymphocyte and complement activation. Regarding vascular remodeling pathways, we observed activation of collagen metabolic processes, fibroblast proliferation, angiogenesis, and smooth muscle cell proliferation in the intima and media. Our pharmacogenomic network analysis identified genes that could potentially be targeted by currently approved treatments or new immunotherapies.Conclusion: Fibroblasts appear to be involved in the GCA process. Our results provide the first characterization by in situ spatial profiling of the molecular players involved in GCA, essential for the discovery of potential new therapeutic targets to treat this vasculitis
Van, Leen Eric. "On the morphogenesis of the D. melanogaster pupa : a study on gene patterning and tissue folding." Electronic Thesis or Diss., Sorbonne université, 2020. http://www.theses.fr/2020SORUS387.
Full textIn order to achieve complex shapes during development, multicellular organisms need to coordinate cellular behaviors to form complex and functional organs. Identifying genes that are expressed in patterns that correlate with cellular processes is therefore primordial. Using the dorsal epithelium (the notum) of drosophila pupa as a model, my thesis aimed at uncovering the molecular mechanisms which control the spatial regulation of morphogenesis at the cell and tissue scale. First, I developed spatial transcriptomics which enabled the identification of new expression patterns involved in notum morphogenesis. Second, I developed, in collaboration with the imaging platform of Institut Curie, Rotating Sample Confocal Microscopy. Using this technique, I was able to simultaneously observe the morphogenesis of the notum, hinge and wing blade. This enabled the discovery of a new morphogenetic movement in the notum between 45-50hAPF. My results suggest that this extensive folding and elongation of the notum is independent of folding in the wing. Furthermore, I demonstrated that the expression of serine proteases regulate the attachment of the tissue to the cuticle which triggers the onset of the folding and determines the final shape of the tissue. Overall, this work increases our understanding of the spatial regulation of morphogenesis and contributes to the knowledge on how the extracellular matrix can regulate tissue shape
Jestin, Martin. "Modifications du microenvironnement stromal après irradiation localisée du côlon : identification de voies moléculaires pour optimiser le processus de régénération épithéliale." Electronic Thesis or Diss., Sorbonne université, 2024. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2024SORUS165.pdf.
Full textPelvic cancers are highly prevalent and are mainly treated with radiotherapy. While radiation therapy may control the tumor, it can also cause damage to surrounding healthy tissue, leading to disabling complications defined as a disease “pelvic radiation disease” (PRD). Currently, there is no curative treatment for this fibrosing pathology. The aims of this project are to study the colonic microenvironment after irradiation with a view to identify new therapeutic targets to improve the management of the colonic sequelae of PRD. For this project, a mouse model developing fibrosing colonic lesions similar to those observed in PRD patients was developed. It consists of localized colorectal irradiation with a single dose of 26Gy. We defined 2 post-irradiation study periods: 2 weeks to study the acute effects of irradiation and the regeneration process, and 12 weeks to study fibrosis. Histological studies characterized the mucosal lesions, with a deep ulcer at 2 weeks and fibrous remodeling at 12 weeks. At the 2 time points studied, an increased and disorganized proliferative process was observed, as well as a deficit in epithelial junction proteins, suggesting a defect in barrier function. We demonstrated the impact of the irradiated colonic microenvironment on epithelial proliferation and differentiation processes using a co-culture system with colonic organoids monitored by video microscopy. Our results validated in vivo observations of increased organoid proliferation in the presence of stroma derived from mice 12 weeks post-irradiation.To characterize stromal mesenchymal cells after irradiation, single-cell RNA sequencing experiments (using EpCAM-CD45-sorted colonic cells and from whole colon) and spatial transcriptomics were performed. They revealed a new marker, Edil3, specific for the major stromal population of the colon. This new marker allowed us to better characterize this cell population in terms of function and localization in the healthy colon. We proposed to call them mesitocytes. In the early stages, we found that this population could differentiate towards a pro-inflammatory profile called "IAF" for "Inflammation-Associated Fibroblasts". We also observed increased expression of transcripts involved in critical functions such as epithelial homeostasis, angiogenesis and inflammation by the majority of mesenchymal cells. The results demonstrate the importance of proliferative molecular signals from lymphatic endothelial cells and smooth muscle cells, particularly Grem-1. Analysis of the chronic phase after irradiation confirms the increase in proliferative signals from stromal cells. In addition, a new fibroblast cell type associated with fibrosis was observed, characterized by a transcriptional profile different from that of the IAF observed in the early phase. The study of the effects of irradiation on the epithelial compartment revealed significant changes in the colonocyte population and the appearance of epithelial cells with a "revival" phenotype, already described in the literature. Interestingly, these populations have specific localizations in regenerating crypts. We also established the importance of genes such as Lypd8 and Anxa1 in the progression of proliferating epithelial cells towards a "revival" phenotype. Interesting observations from spatial transcriptomic analyses also allow us to hypothesize the role of immune cells in the epithelial regeneration process