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Journal articles on the topic 'Tradescantia micronuclei test'

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Author: Grafiati

Published: 11 March 2023

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1

Falistocco, E., R. Torricelli, D. Feretti, I. Zerbini, C. Zani, and S. Monarca. "Enhancement of Micronuclei Frequency in the Tradescantia/Micronuclei Test Using a Long Recovery Time." Hereditas 133, no. 2 (May 5, 2004): 171–74. http://dx.doi.org/10.1111/j.1601-5223.2000.t01-1-00171.x.

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2

Techio, V. H., J. Stolberg, A. Kunz, E. Zanin, and C. C. Perdomo. "Genotoxicity of swine effluents." Water Science and Technology 63, no. 5 (March 1, 2011): 970–76. http://dx.doi.org/10.2166/wst.2011.278.

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This study aimed at the investigation of genotoxic effects of swine effluents from different stages of a treatment system for swine wastes through bioassay of stamen hairs and micronuclei in Tradescantia (clone BNL 4430). No significant differences (p≥0.05) regarding the genic mutations were found in the bioassay of stamen hairs, independently of the effluent analysed. For the genotoxicity test with micronuclei, the plants exposed to raw wastes, to sludge, and to effluent of the biodigester have presented higher rates of chromosomal damages (micronuclei), with significant differences in relation to the control group and other effluent of the waste treatment system (p≤0.05). The association between the chemical parameters and the genotoxicity data have shown that the variables COD and TKN have presented significant correlation (p≤0.05) with the number of mutagenic events in the tetrads.

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3

Meireles, José, Rodrigo Rocha, Antônio Costa Neto, and Eneida Cerqueira. "Genotoxic effects of vehicle traffic pollution as evaluated by micronuclei test in tradescantia (Trad-MCN)." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 675, no. 1-2 (April 2009): 46–50. http://dx.doi.org/10.1016/j.mrgentox.2009.02.005.

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4

Monarca, S., D. Feretti, I. Zerbini, C. Zani, A. Alberti, S. D. Richardson, A. D. Thruston, P. Ragazzo, and L. Guzzella. "Studies on mutagenicity and disinfection by-products in river drinking water disinfected with peracetic acid or sodium hypochlorite." Water Supply 2, no. 3 (July 1, 2002): 199–204. http://dx.doi.org/10.2166/ws.2002.0103.

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The aim of this research was to study at a pilot plant the influence of peracetic acid (PAA) on the formation of mutagenic compounds in river waters used for human consumption. The results obtained using PAA were compared to those for the most commonly used disinfectant, sodium hypochlorite (NaClO). Ames test and three genotoxicity plant tests, Allium tests and Tradescantia/micronuclei (TRAD/MCN) test, were used to evaluate the mutagenic activity of disinfected water samples. Chemical analysis, using gas chromatography/mass spectrometry (GC/MS), was also performed to identify disinfection by-products (DBPs). A slight bacterial mutagenicity was found in raw river water and similar activity was detected in disinfected water samples. Plant tests gave genotoxicity only for raw river water. DBPs identified in PAA-treated water included carboxylic acids, a few non-halogenated alcohols and carbonyl-containing compounds, whereas some potentially mutagenic halogenated by-products were found in NaClO-treated samples. Although PAA appears to be promising for water potabilization, these results must be confirmed with different source waters and with higher concentrations of PAA.

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5

Pavani, Julio Becker, Isadora Coelho Zaccaron, Pedro Gabriel Ambrosio, Kristian Madeira, Paula Tramontim Pavei, Marta Valéria Guimarães De Souza Hoffmann, Guilherme De Sá, Cristina Keiko Yamaguchi, and Miriam da Conceição Martins. "BIOMONITORATION OF AREAS PRODUCING CERAMIC MANUFACTURES IN TWO MUNICIPALITIES OF THE SOUTH OF BRAZIL." International Journal for Innovation Education and Research 8, no. 2 (February 1, 2020): 188–98. http://dx.doi.org/10.31686/ijier.vol8.iss2.2185.

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Industrial and urban development, together with population growth, has contributed to the increase of atmospheric pollution, and causes the air of urban centers to present more and more substances that are harmful to living beings. In southern Santa Catarina, Morro da Fumaça and Sangão are the highlights in the production of red ceramics (bricks and tiles), these industries use combustion processes for the manufacture of their product and releases air pollutants.The exposure period of the organism was from June to October 2015, at Points A and B in Morro da Fumaça and C, D and E in Sangão. Collections of the young inflorescences of the exposed plants were carried out weekly. After collection, inflorescences were fixed in ethanol / glacial acetic acid solution (3: 1) for 24 hours, transferred to 70% ethanol solution and stored under refrigeration. These were submitted to staining with acetic carmine and the number of micronuclei in 300 tetrads was quantified for each inflorescence, and blades with 5 inflorescences / week were made for each point studied. The results were expressed as mean ± standard deviation and median and range. In the present study it can be verified that the bioindicator Tradescantia pallida shows to be a highly sensitive plant and an excellent test organism, considered an essential tool in the biomonitoring of polluted environments. The results show that in the month of August, the frequency of micronuclei was significantly higher in the plants of the sampling points.

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6

BISCARDI, D., S. MONARCA, R. DEFUSCO, F. SENATORE, P. POLI, A. BUSCHINI, C. ROSSI, and C. ZANI. "Evaluation of the migration of mutagens/carcinogens from PET bottles into mineral water by Tradescantia/micronuclei test, Comet assay on leukocytes and GC/MS." Science of The Total Environment 302, no. 1-3 (January 20, 2003): 101–8. http://dx.doi.org/10.1016/s0048-9697(02)00349-2.

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7

Ma, Te-Hsiu, Van A. Anderson, Mary M. Harris, Robert E. Neas, and Tze-San Lee. "Mutagenicity of drinking water detected by the Tradescantia micronucleus test." Canadian Journal of Genetics and Cytology 27, no. 2 (April 1, 1985): 143–50. http://dx.doi.org/10.1139/g85-022.

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Spring Lake reservoir of Macomb, Illinois, is a typical model of the drinking water supply of some midwestern towns of the United States. Water samples collected periodically in 1980 and 1981 from this lake were tested for mutagenicity using the Tradescantia micronucleus (Trad-MCN) test, a highly sensitive mutagen-detecting bioassay. Water samples from 1981 were also analyzed chemically. The micronucleus (MCN) frequency peaked (12–14 MCN/100 tetrads) in mid-July in both years, as compared with the average frequency (5 MCN/100 tetrads) of the base-line control that was maintained in nutrient solution (prepared with distilled water and pure chemicals). Drinking water from the tap was tested in parallel with lake water, and its mutagenicity tended to fluctuate with the mutagenicity of the lake water.Key words: mutagenicity, drinking water, Tradescantia, micronucleus.

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8

Wang, Shuyu, and Xunling Wang. "The Tradescantia-micronucleus test on the genotoxicity of UV-B radiation." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 426, no. 2 (May 1999): 151–53. http://dx.doi.org/10.1016/s0027-5107(99)00059-7.

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9

Araújo, Ana Lúzia de Souza, Eliane Tigre Guimarães, and Robson Seriani. "MUTAGENESIS IN Tradescantia pallida AS A BIOMARKER OF THE EFFECTS OF WATER POLLUTED WITH URBAN EFFLUENT." Holos Environment 14, no. 1 (June 18, 2014): 97. http://dx.doi.org/10.14295/holos.v14i1.6911.

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The present paper evaluated the toxicity of effluent released into the Tiete River, in Barra Bonita City, São Paulo State, using the test micronucleus with Tradescantia pallida. Young inflorescences of T. pallida (n = 20) were partially immersed in one of three treatments: water containing effluent, a positive control (0.1% formaldehyde), and a negative control (distilled water). The results showed a statistically significant difference (p < 0.05) between groups, indicating the genotoxicity of the effluent.

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10

Klumpp, Andreas, Wolfgang Ansel, Gabriele Klumpp, Vicent Calatayud, Jean Pierre Garrec, Shang He, Josep Peñuelas, et al. "Tradescantia micronucleus test indicates genotoxic potential of traffic emissions in European cities." Environmental Pollution 139, no. 3 (February 2006): 515–22. http://dx.doi.org/10.1016/j.envpol.2005.05.021.

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11

de Morais, Cássio Resende, Boscolli Barbosa Pereira, Patrícia Cristina Almeida Sousa, Vanessa Santana Vieira Santos, Carlos Fernando Campos, Stephan Malfitano Carvalho, Mário Antônio Spanó, Alexandre Azenha Alves de Rezende, and Ana Maria Bonetti. "Evaluation of the genotoxicity of neurotoxic insecticides using the micronucleus test in Tradescantia pallida." Chemosphere 227 (July 2019): 371–80. http://dx.doi.org/10.1016/j.chemosphere.2019.04.073.

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12

Lah, B., T. Vidic, E. Glasencnik, T. Cepeljnik, G. Gorjanc, and Romana Marinsek-Logar. "Genotoxicity evaluation of water soil leachates by Ames test, comet assay, and preliminary Tradescantia micronucleus assay." Environmental Monitoring and Assessment 139, no. 1-3 (June 14, 2007): 107–18. http://dx.doi.org/10.1007/s10661-007-9819-7.

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13

Baud-Grasset, Sandrine, Frédéric Baud-Grasset, Joseph M. Bifulco, John R. Meier, and Te-Hsiu Ma. "Reduction of genotoxicity of a creosote-contaminated soil after fungal treatment determined by the Tradescantia-micronucleus test." Mutation Research Letters 303, no. 2 (October 1993): 77–82. http://dx.doi.org/10.1016/0165-7992(93)90098-g.

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14

Mišík, Miroslav, Karol Mičieta, Martina Solenská, Nataša Markusková, Katarína Mišíková, and Siegfried Knasmüller. "In situ monitoring of the genotoxic effects in the vicinity of a petrochemical plant with the Tradescantia micronucleus (Trad-MCN) and with pollen abortion test." BMC Pharmacology 7, Suppl 2 (2007): A53. http://dx.doi.org/10.1186/1471-2210-7-s2-a53.

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15

Avalyan, R., A. Atoyants, L. Hambaryan, E. Aghajanyan, B. Gabrielyan, and R. Aroutiounian. "ASSESSMENT OF THE STATE OF LAKE SEVAN WATER DURING THE SUMMER USING MODEL TEST-OBJECTS." Transactions of Papanin Institute for Biology of Inland Waters RAS, June 30, 2022, 26–37. http://dx.doi.org/10.47021/0320-3557-2022-26-37.

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The biotesting of the level of genotoxicity and clastogenicity of deep and surface waters of Lake Sevan (Armenia) in the summer period was carried out using two test systems: of the model test object Tradescantia (clone 02) and phytoplankton. The Tradescantia clone 02 stamen hair mutations (Trad-SHM) and Tradescantia micronucleus (Trad-MCN) bioassays are two of the efficient and reliable biomonotory test systems for mutagenicity of air, water and soil polutans. Here we report a significant increase in the level of The Tradescantia clone 02 stamen hair mutations (Trad-SHM) somatic mutations in all investigated probes compared to phone control level. The maximum manifestation of these genetic effects – PC was observed in Gilli water samples with five-fold increase. According to the micronucleus test (Trad-MN), when studying its two main test-criteria: the percentage of tetrads with micronuclei and micronuclei in tetrads, the maximum frequency of test indicators was detected in water samples of Tsapatakh and Arpa, where their level was 4 times higher than the background. A significant positive correlation between the somatic mutation events and consentration of chemical elements Ca, K, Na, Mg, Fe, Mo (p<0.05; p<0.001) was revealed. It also showed a significant correlation between MN in tetrads and consentration of chemical elements Na, Ca, Fe, Mo, Ni, Cd (p<0.05). The analysis of the spatial distribution of phytoplankton in the water area of the Lake Sevan, as well as data on the abundance, biomass and composition of the dominant species in the summer observation period showed an intensive development of Cyanoprokaryota (HAB) and green algae in the all water area of the lake, which caused the process of its eutrophication.

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16

Salgueiro, S. A. M., A. N. Rocha, J. R. C. Mauad, C. A. M. Silva, and R. M. Mussury. "Biomonitoring of air quality in the Bodoquena microregion, Mato Grosso Do Sul: mutagenic and morphoanatomical alterations in Tradescantia pallida (rose) D.R. Hunt var. purpurea." Brazilian Journal of Biology 84 (2024). http://dx.doi.org/10.1590/1519-6984.250100.

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Abstract The objective of this study was to assess air quality in relation to vehicular traffic flow in cities located at different elevations in the Bodoquena microregion, state of Mato Grosso do Sul, Brazil. To do so, a micronucleus test was carried out using the TRAD-MCN bioassay on young Tradescantia buds collected from February to November 2018 in seven cities of the microregion with different traffic flow intensities. Meteorological parameters were evaluated, and vehicular traffic was counted to determine traffic flow in each city. With data from the Shuttle Radar Topography Mission (SRTM) and processing in Esri ArcGIS® software version 10.5.1, the regions was mapped based on an Elevation Model. Morphoanatomical analyses were performed according to standard methodology. Measurements were taken of thickness, length and width of tissues and structures, including the upper and lower cuticle, upper and lower epidermis, hypodermis and mesophyll. The greatest traffic flow was found in the cities of Bodoquena, Guia Lopes da Laguna, Jardim, and Porto Murtinho, with the period from 5:00 to 6:00 p.m. showing the highest traffic flow. The greatest frequency of mutagenic alterations was found in the city of Guia Lopes da Laguna, although the results did not differ significantly from Bonito, Caracol, and Jardim. Throughout the biomonitoring, the summer and autumn seasons showed the greatest micronuclei frequencies in all evaluated cities. Variations in the tissue/structure thickness was observed across cities and seasons, but with a decrease in thickness during autumn. In general, the tissues/structures were smaller for the cities of Nioaque and Porto Murtinho, while the anatomical and morphological characteristics of leaf length and thickness showed no differences among cities. We found limited correlation between micronuclei frequency and traffic flow, supporting the hypothesis that although mutagenic alterations are observed in T. pallida, in this microregion the changes are numerically lower when compared to other regions of the state. In light of the genotoxic and morphoanatomical factors assessed herein, the Bodoquena microregion appears to be well preserved in terms of air quality, presenting low micronuclei frequency and a limited reduction in tissues and leaf structures, regardless of the season.

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17

"Final Report on the Safety Assessment of Biotin." International Journal of Toxicology 20, no. 4_suppl (January 2001): 1–12. http://dx.doi.org/10.1080/109158101529025472.

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Biotin is a water-soluble vitamin used as a hair-conditioning agent and a skin-conditioning agent in many cosmetic products at concentrations ranging from 0.0001% to 0.6%. Although Biotin does absorb some ultraviolet (UV) radiation, the absorption shows no peaks in the UVA or UVB region. Biotin is rapidly metabolized and excreted in urine. Little acute oral toxicity is seen in animal tests. Short-term and subchronic toxicity studies likewise found no evidence of toxicity. Although intradermal injection of a small quantity of Biotin (0.1 ml) into guinea pig skin did not produce skin irritation, Biotin (0.1% at pH 7.3) did produce slight, transient ocular irritation in rabbit eyes. Biotin was not mutagenic in bacterial tests, but positive results were found in a Tradescantia micronucleus test. There was evidence of an increase in the number of resorptions in rats receiving Biotin by subcutaneous injection, with concomitant decreases in fetal, uterine, and placental weights. Another study of mice receiving Biotin orally or by subcutaneous injection found no differences between control and treatment groups. Although there is one case study reporting an urticarial reaction in the literature, there are a very large number of individuals exposed to Biotin on a daily basis, and there is not a parallel appearance of irritation, sensitization, or other adverse reactions. Based on these available data, it was concluded that Biotin is safe as used in cosmetic formulations.

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