Journal articles: 'Toxicity, behaviour, zebrafish'

1

Hung, Ming Wai, Zai Jun Zhang, Shang Li, Benson Lei, Shuai Yuan, Guo Zhen Cui, Pui Man Hoi, Kelvin Chan, and Simon Ming Yuen Lee. "From Omics to Drug Metabolism and High Content Screen of Natural Product in Zebrafish: A New Model for Discovery of Neuroactive Compound." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–20. http://dx.doi.org/10.1155/2012/605303.

Full text

Abstract:

The zebrafish (Danio rerio) has recently become a common model in the fields of genetics, environmental science, toxicology, and especially drug screening. Zebrafish has emerged as a biomedically relevant model forin vivohigh content drug screening and the simultaneous determination of multiple efficacy parameters, including behaviour, selectivity, and toxicity in the content of the whole organism. A zebrafish behavioural assay has been demonstrated as a novel, rapid, and high-throughput approach to the discovery of neuroactive, psychoactive, and memory-modulating compounds. Recent studies found a functional similarity of drug metabolism systems in zebrafish and mammals, providing a clue with why some compounds are active in zebrafishin vivobut notin vitro, as well as providing grounds for the rationales supporting the use of a zebrafish screen to identify prodrugs. Here, we discuss the advantages of the zebrafish model for evaluating drug metabolism and the mode of pharmacological action with the emerging omics approaches. Why this model is suitable for identifying lead compounds from natural products for therapy of disorders with multifactorial etiopathogenesis and imbalance of angiogenesis, such as Parkinson's disease, epilepsy, cardiotoxicity, cerebral hemorrhage, dyslipidemia, and hyperlipidemia, is addressed.

APA, Harvard, Vancouver, ISO, and other styles

2

Bilberg, Katrine, Mads Bruun Hovgaard, Flemming Besenbacher, and Erik Baatrup. "In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)." Journal of Toxicology 2012 (2012): 1–9. http://dx.doi.org/10.1155/2012/293784.

Full text

Abstract:

The influence of water chemistry on characterised polyvinyl pyrrolidone- (PVP-) coated silver nanoparticles (81 nm) was investigated. NaCl solution series of 100–800 mg L−1lead to initial and temporal increase in nanoparticles size, but agglomeration was limited. pH variation (5–8) had only minor influence on the hydrodynamic particle size. Acute toxicity of nanosivler to zebrafish (Danio rerio) was investigated in a 48-hour static renewal study and compared with the toxicity of silver ions (AgNO3). The nanosilver and silver ion 48-hour median lethal concentration (LC50) values were 84 μg L−1and 25 μg L−1, respectively. To investigate exposure-related stress, the fish behaviour was observed visually after 0, 3, 6, 12, 24, 27, 30, and 48 hours of both nanosilver and ionic silver treatments. These observations revealed increased rate of operculum movement and surface respiration after nanosilver exposure, suggesting respiratory toxicity. The present study demonstrates that silver nanoparticles are lethal to zebrafish.

APA, Harvard, Vancouver, ISO, and other styles

3

Neylon, Jeremy, Jarrad N. Fuller, Chris van der Poel, Jarrod E. Church, and Sebastian Dworkin. "Organophosphate Insecticide Toxicity in Neural Development, Cognition, Behaviour and Degeneration: Insights from Zebrafish." Journal of Developmental Biology 10, no. 4 (November 21, 2022): 49. http://dx.doi.org/10.3390/jdb10040049.

Full text

Abstract:

Organophosphate (OP) insecticides are used to eliminate agricultural threats posed by insects, through inhibition of the neurotransmitter acetylcholinesterase (AChE). These potent neurotoxins are extremely efficacious in insect elimination, and as such, are the preferred agricultural insecticides worldwide. Despite their efficacy, however, estimates indicate that only 0.1% of organophosphates reach their desired target. Moreover, multiple studies have shown that OP exposure in both humans and animals can lead to aberrations in embryonic development, defects in childhood neurocognition, and substantial contribution to neurodegenerative diseases such as Alzheimer’s and Motor Neurone Disease. Here, we review the current state of knowledge pertaining to organophosphate exposure on both embryonic development and/or subsequent neurological consequences on behaviour, paying particular attention to data gleaned using an excellent animal model, the zebrafish (Danio rerio).

APA, Harvard, Vancouver, ISO, and other styles

4

Yang, Mengying, Jinyu Qiu, Xin Zhao, and XiZeng Feng. "6-benzylaminopurine exposure induced development toxicity and behaviour alteration in zebrafish (Danio rerio)." Environmental Pollution 278 (June 2021): 116887. http://dx.doi.org/10.1016/j.envpol.2021.116887.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Wang, Honglei, Liqun Zhou, Zhen Meng, Meile Su, Shouhua Zhang, Pinzhen Huang, Fen Jiang, Xinjun Liao, Zigang Cao, and Huiqiang Lu. "Clethodim exposure induced development toxicity and behaviour alteration in early stages of zebrafish life." Environmental Pollution 255 (December 2019): 113218. http://dx.doi.org/10.1016/j.envpol.2019.113218.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Sárria, M. P., J. Soares, M. N. Vieira, L. Filipe C. Castro, M. M. Santos, and N. M. Monteiro. "Rapid-behaviour responses as a reliable indicator of estrogenic chemical toxicity in zebrafish juveniles." Chemosphere 85, no. 10 (November 2011): 1543–47. http://dx.doi.org/10.1016/j.chemosphere.2011.07.048.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Thayumanavan, Geethanjali, Srikanth Jeyabalan, Shivkanya Fuloria, Mahendran Sekar, Monica Ravi, Logesh Kumar Selvaraj, Logeshwari Bala, et al. "Silibinin and Naringenin against Bisphenol A-Induced Neurotoxicity in Zebrafish Model—Potential Flavonoid Molecules for New Drug Design, Development, and Therapy for Neurological Disorders." Molecules 27, no. 8 (April 15, 2022): 2572. http://dx.doi.org/10.3390/molecules27082572.

Full text

Abstract:

Bisphenol A (BPA), a well-known xenoestrogen, is commonly utilised in the production of polycarbonate plastics. Based on the existing evidence, BPA is known to induce neurotoxicity and behavioural issues. Flavonoids such as silibinin and naringenin have been shown to have biological activity against a variety of illnesses. The current research evaluates the neuropharmacological effects of silibinin and naringenin in a zebrafish model against neurotoxicity and oxidative stress caused by Bisphenol A. In this study, a novel tank diving test (NTDT) and light–dark preference test (LDPT) were used in neurobehavioural investigations. The experimental protocol was planned to last 21 days. The neuroprotective effects of silibinin (10 μM) and naringenin (10 μM) in zebrafish (Danio rerio) induced by BPA (17.52 μM) were investigated. In the brine shrimp lethality assay, the 50% fatal concentrations (LC50) were 34.10 μg/mL (silibinin) and 91.33 μg/mL (naringenin) compared to the standard potassium dichromate (13.15 μg/mL). The acute toxicity investigation found no mortality or visible abnormalities in the silibinin- and naringenin-treated groups (LC50 > 100 mg/L). The altered scototaxis behaviour in LDPT caused by BPA was reversed by co-supplementation with silibinin and naringenin, as shown by decreases in the number of transitions to the light zone and the duration spent in the light zone. Our findings point to BPA’s neurotoxic potential in causing altered scototaxis and bottom-dwelling behaviour in zebrafish, as well as the usage of silibinin and naringenin as potential neuroprotectants.

APA, Harvard, Vancouver, ISO, and other styles

8

Gupta, Govind Sharan, Krupa Kansara, Helly Shah, Ruchi Rathod, Drishti Valecha, Saurabh Gogisetty, Pankti Joshi, and Ashutosh Kumar. "Impact of humic acid on the fate and toxicity of titanium dioxide nanoparticles in Tetrahymena pyriformis and zebrafish embryos." Nanoscale Advances 1, no. 1 (2019): 219–27. http://dx.doi.org/10.1039/c8na00053k.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Pérez-Davila, Sara, Laura González-Rodríguez, Raquel Lama, Miriam López-Álvarez, Ana Leite Oliveira, Julia Serra, Beatriz Novoa, Antonio Figueras, and Pío González. "3D-Printed PLA Medical Devices: Physicochemical Changes and Biological Response after Sterilisation Treatments." Polymers 14, no. 19 (October 1, 2022): 4117. http://dx.doi.org/10.3390/polym14194117.

Full text

Abstract:

Polylactic acid (PLA) has become one of the most commonly used polymers in medical devices given its biocompatible, biodegradable and bioabsorbable properties. In addition, due to PLA’s thermoplastic behaviour, these medical devices are now obtained using 3D printing technologies. Once obtained, the 3D-printed PLA devices undergo different sterilisation procedures, which are essential to prevent infections. This work was an in-depth study of the physicochemical changes caused by novel and conventional sterilisation techniques on 3D-printed PLA and their impact on the biological response in terms of toxicity. The 3D-printed PLA physicochemical (XPS, FTIR, DSC, XRD) and mechanical properties as well as the hydrophilic degree were evaluated after sterilisation using saturated steam (SS), low temperature steam with formaldehyde (LTSF), gamma irradiation (GR), hydrogen peroxide gas plasma (HPGP) and CO2 under critical conditions (SCCO). The biological response was tested in vitro (fibroblasts NCTC-929) and in vivo (embryos and larvae wild-type zebrafish Danio rerio). The results indicated that after GR sterilisation, PLA preserved the O:C ratio and the semi-crystalline structure. Significant changes in the polymer surface were found after HPGP, LTSF and SS sterilisations, with a decrease in the O:C ratio. Moreover, the FTIR, DSC and XRD analysis revealed PLA crystallisation after SS sterilisation, with a 52.9% increase in the crystallinity index. This structural change was also reflected in the mechanical properties and wettability. An increase in crystallinity was also observed after SCCO and LTSF sterilisations, although to a lesser extent. Despite these changes, the biological evaluation revealed that none of the techniques were shown to promote the release of toxic compounds or PLA modifications with toxicity effects. GR sterilisation was concluded as the least reactive technique with good perspectives in the biological response, not only at the level of toxicity but at all levels, since the 3D-printed PLA remained almost unaltered.

APA, Harvard, Vancouver, ISO, and other styles

10

Rosa, João Gabriel Santos, Carla Lima, and Monica Lopes-Ferreira. "Zebrafish Larvae Behavior Models as a Tool for Drug Screenings and Pre-Clinical Trials: A Review." International Journal of Molecular Sciences 23, no. 12 (June 14, 2022): 6647. http://dx.doi.org/10.3390/ijms23126647.

Full text

Abstract:

To discover new molecules or review the biological activity and toxicity of therapeutic substances, drug development, and research relies on robust biological systems to obtain reliable results. Phenotype-based screenings can transpose the organism’s compensatory pathways by adopting multi-target strategies for treating complex diseases, and zebrafish emerged as an important model for biomedical research and drug screenings. Zebrafish’s clear correlation between neuro-anatomical and physiological features and behavior is very similar to that verified in mammals, enabling the construction of reliable and relevant experimental models for neurological disorders research. Zebrafish presents highly conserved physiological pathways that are found in higher vertebrates, including mammals, along with a robust behavioral repertoire. Moreover, it is very sensitive to pharmacological/environmental manipulations, and these behavioral phenotypes are detected in both larvae and adults. These advantages align with the 3Rs concept and qualify the zebrafish as a powerful tool for drug screenings and pre-clinical trials. This review highlights important behavioral domains studied in zebrafish larvae and their neurotransmitter systems and summarizes currently used techniques to evaluate and quantify zebrafish larvae behavior in laboratory studies.

APA, Harvard, Vancouver, ISO, and other styles

11

Montes, Guilherme Carneiro. "The use of zebrafish to evaluate neuropharmacology of the gold nanoparticles." European Journal of Chemistry 12, no. 4 (December 31, 2021): 488–92. http://dx.doi.org/10.5155/eurjchem.12.4.488-492.2152.

Full text

Abstract:

Zebrafish (Danio rerio) is a vertebrate animal used in animal model research with complex brains and behaviors similar to humans and associate with low coast become a model attractive for the academic community to seek zebrafish for scientific research. Studies on diseases of the central nervous system (CNS) have advanced and news therapeutic agents were developed for treatment these disorders. Reports suggest that the zebrafish model supports the neurodegenerative studies due functional conservation between human genes implicated in neurodegenerative disorders. The discovery of therapeutic compounds for CNS using the zebrafish model allows to show a neuroprotective action or neurotoxicity that might alter the behavioral changes. Neurotoxicity tests might perform in zebrafish’s embryos into 96 multi-well plates, which reduces the amount of substances used and cost. The bioactive compounds able to penetrate the blood-brain barrier (BBB) have important role physicochemical properties that might be desirable pharmacological effects and zebrafish trials allow if the substances might penetrate BBB and to exert central activity. The assays zebrafish are used to analyze nanoparticles that are small molecules used to explore variety applications in human health. Gold nanoparticles (AuNPs) has important properties which are extremely interest for pharmaceutical area such as drug delivery, cellular imaging, diagnostics, and therapeutic agents. Gold nanoparticles enhances Parkinson symptoms and improved neuroinflammation. Some studies show zebrafish might use to evaluate gold nanoparticles for human health hazard and toxicity studies. There is enormous potential for zebrafish in preclinical assays due to predict pharmacological and toxicity effects. Specific guidelines focused on methodologies in the zebrafish are needed to ensure adequate reproducible trials.

APA, Harvard, Vancouver, ISO, and other styles

12

Lee, Wang, Eungwang Kim, Hyun-Ju Cho, Taejoon Kang, Bongsoo Kim, Min Kim, Yong Kim, Nam Song, Jeong-Soo Lee, and Jinyoung Jeong. "The Relationship between Dissolution Behavior and the Toxicity of Silver Nanoparticles on Zebrafish Embryos in Different Ionic Environments." Nanomaterials 8, no. 9 (August 23, 2018): 652. http://dx.doi.org/10.3390/nano8090652.

Full text

Abstract:

A silver nanoparticle is one of the representative engineered nanomaterials with excellent optical, electrical, antibacterial properties. Silver nanoparticles are being increasingly used for medical products, water filters, and cosmetics, etc. However, silver nanoparticles are known to cause adverse effects on the ecosystem and human health. To utilize silver nanoparticles with minimized negative effects, it is important to understand the behavior of silver nanoparticles released to the environment. In this study, we compared toxicity behaviors of citrate-stabilized silver nanoparticles with polyethylene glycol coated silver nanoparticles in two different ionic environments, which are aquatic environments for developing zebrafish embryo. Depending on the composition of the ionic environment, citrate-stabilized silver nanoparticles and polyethylene glycol coated silver nanoparticles exhibited different behaviors in dissolution, aggregation, or precipitation, which governed the toxicity of silver nanoparticles on zebrafish embryos.

APA, Harvard, Vancouver, ISO, and other styles

13

Horzmann, Katharine A., Ana M. Portales, Kathryn G. Batcho, and Jennifer L. Freeman. "Developmental toxicity of trichloroethylene in zebrafish (Danio rerio)." Environmental Science: Processes & Impacts 22, no. 3 (2020): 728–39. http://dx.doi.org/10.1039/c9em00565j.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Kim, MJ, J. Choi, N. Kim, and GC Han. "Behavioral changes of zebrafish according to cisplatin-induced toxicity of the balance system." Human & Experimental Toxicology 33, no. 11 (February 6, 2014): 1167–75. http://dx.doi.org/10.1177/0960327114521046.

Full text

Abstract:

Background and objectives: Zebrafish are commonly used as experimental animals in otolaryngology studies. However, the behavioral characteristics of these fish are not well known, especially those related to the vestibular system. The goal of this study was to evaluate behavioral changes in zebrafish due to toxicity in the balance system. Materials and methods: Zebrafish were exposed to 1000 μM cisplatin for 6 h. We, then, periodically monitored swimming depth, total swimming distance, peak swimming velocity, and mean swimming velocity of the fish for approximately 21 days. Results: Total swimming distance ( p < 0.0001), peak swimming velocity ( p = 0.0063), and mean swimming velocity ( p < 0.0001) in the cisplatin-administered group were significantly decreased when compared with control fish. Conclusion: Our findings demonstrate that cisplatin can alter the locomotion behavior of zebrafish.

APA, Harvard, Vancouver, ISO, and other styles

15

Wu, Yuanzhao, Anli Wang, Lixiang Fu, Meng Liu, Kang Li, Song Chian, Weixuan Yao, Binjie Wang, and Jiye Wang. "Fentanyl Induces Novel Conditioned Place Preference in Adult Zebrafish, Disrupts Neurotransmitter Homeostasis, and Triggers Behavioral Changes." International Journal of Environmental Research and Public Health 19, no. 20 (October 19, 2022): 13533. http://dx.doi.org/10.3390/ijerph192013533.

Full text

Abstract:

Abuse of new psychoactive substances increases risk of addiction, which can lead to serious brain disorders. Fentanyl is a synthetic opioid commonly used in clinical practice, and behavioral changes resulting from fentanyl addiction have rarely been studied with zebrafish models. In this study, we evaluated the rewarding effects of intraperitoneal injections of fentanyl at concentrations of 10, 100, and 1000 mg/L on the group shoaling behavior in adult zebrafish. Additional behavioral tests on individual zebrafish, including novel tank, novel object exploration, mirror attack, social preference, and T-maze memory, were utilized to evaluate fentanyl-induced neuro-behavioral toxicity. The high doses of 1000 mg/L fentanyl produced significant reward effects in zebrafish and altered the neuro-behavioral profiles: reduced cohesion in shoaling behavior, decreased anxiety levels, reduced exploratory behavior, increased aggression behavior, affected social preference, and suppressed memory in an appetitive associative learning task. Behavioral changes in zebrafish were shown to be associated with altered neurotransmitters, such as elevated glutamine (Gln), gamma-aminobutyric acid (GABA), dopamine hydrochloride (DA), and 5-hydroxytryptamine (5-HT). This study identified potential fentanyl-induced neurotoxicity through multiple neurobehavioral assessments, which provided a method for assessing risk of addiction to new psychoactive substances.

APA, Harvard, Vancouver, ISO, and other styles

16

Doan, Hau, Pishyaporn Sritangos, Oratai Weeranantanapan, and Nuannoi Chudapongse. "Aqueous extract from Chrysophyllum cainito bark exhibits embryonic toxicity in Danio rerio and negligible acute toxicity in adult wistar rats." Archives of Biological Sciences 73, no. 4 (2021): 523–33. http://dx.doi.org/10.2298/abs211110046d.

Full text

Abstract:

Chrysophyllum cainito has been used as a traditional medicine to treat a wide range of diseases, but the toxicity profile of this plant remains unknown. This study aimed to evaluate the acute toxicity of the aqueous extract of C. cainito (CE) bark based on OECD guidelines in two different in vivo experimental models: acute single-dose oral toxicity in adult Wistar rats and the zebrafish embryo acute toxicity test. All concentrations of CE (500-4000 mg/kg) tested during a 14-day period in both male and female rats showed no effect on behavior, body weight, organ weights, biochemical and hematological parameters. In contrast, CE significantly delayed zebrafish embryo hatching and decreased embryo survival rates in a dose-dependent manner. Hatched larvae were notably sensitive to CE-induced toxicity compared to unhatched fish embryos. Acridine orange staining showed that CE induced apoptosis in the yolk sac region that is responsible for supplying nutrients to support larval growth and development. According to OECD guidelines, CE was identified as GHS category 5, a substance with low to no acute toxicity. However, as embryotoxicity was observed in zebrafish, CE use during pregnancy should be exercised with caution until further examination of its safety.

APA, Harvard, Vancouver, ISO, and other styles

17

Kent, ML, C. Buchner, C. Barton, and RL Tanguay. "Toxicity of chlorine to zebrafish embryos." Diseases of Aquatic Organisms 107, no. 3 (January 16, 2014): 235–40. http://dx.doi.org/10.3354/dao02683.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Gu, Jie, Liguo Guo, Yuanhui Zhu, Lingling Qian, Lili Shi, Huanchao Zhang, and Guixiang Ji. "Neurodevelopmental Toxicity of Emamectin Benzoate to the Early Life Stage of Zebrafish Larvae (Danio rerio)." International Journal of Molecular Sciences 24, no. 4 (February 13, 2023): 3757. http://dx.doi.org/10.3390/ijms24043757.

Full text

Abstract:

Emamectin benzoate (EMB) is a widely used pesticide and feed additive in agriculture and aquaculture. It easily enters the aquatic environment through various pathways, thus causing adverse effects on aquatic organisms. However, there are no systematic studies regarding the effects of EMB on the developmental neurotoxicity of aquatic organisms. Therefore, the aim of this study was to evaluate the neurotoxic effects and mechanisms of EMB at different concentrations (0.1, 0.25, 0.5, 1, 2, 4 and 8 μg/mL) using zebrafish as a model. The results showed that EMB significantly inhibited the hatching rate, spontaneous movement, body length, and swim bladder development of zebrafish embryos, as well as significantly increased the malformation rate of zebrafish larvae. In addition, EMB adversely affected the axon length of motor neurons in Tg (hb9: eGFP) zebrafish and central nervous system (CNS) neurons in Tg (HuC: eGFP) zebrafish and significantly inhibited the locomotor behavior of zebrafish larvae. Meanwhile, EMB induced oxidative damage and was accompanied by increasing reactive oxygen species in the brains of zebrafish larvae. In addition, gene expression involvement in oxidative stress-related (cat, sod and Cu/Zn-sod), GABA neural pathway-related (gat1, gabra1, gad1b, abat and glsa), neurodevelopmental-related (syn2a, gfap, elavl3, shha, gap43 and Nrd) and swim bladder development-related (foxa3, pbxla, mnx1, has2 and elovlla) genes was significantly affected by EMB exposure. In conclusion, our study shows that exposure to EMB during the early life stages of zebrafish significantly increases oxidative damage and inhibits early central neuronal development, motor neuron axon growth and swim bladder development, ultimately leading to neurobehavioral changes in juvenile zebrafish.

APA, Harvard, Vancouver, ISO, and other styles

19

Xie, Zhongtang, Guanghua Lu, and Yeting Yu. "Early-Stage High-Concentration Thiacloprid Exposure Induced Persistent Behavioral Alterations in Zebrafish." International Journal of Environmental Research and Public Health 19, no. 17 (September 1, 2022): 10920. http://dx.doi.org/10.3390/ijerph191710920.

Full text

Abstract:

As a major neonicotinoid insecticide, thiacloprid (THCP) is frequently detected in aquatic environments worldwide due to its heavy use, posing potential threats to aquatic organisms. In this study, zebrafish (Danio rerio) embryos were exposed to THCP (1, 10, 100, 1000 and 10,000 μg/L) for 5 days and then recovered in THCP-free water for 20 days to investigate the effects of early-stage THCP exposure on the development, antioxidant defense, and neurotransmitter systems of zebrafish, and explore their recovery mechanism. The results show that THCP exposure induced developmental toxicity and oxidative stress in zebrafish. The hypoactivity, behavioral alterations (decreased avoidance and edge preference behaviors) and neurotoxicity were found throughout the exposure-recovery experiments. THCP exposure altered the expression of γ-aminobutyric acid (GABA)- and serotonin (5-HT)-related genes accompanied by the decrease in GABA and 5-HT contents. However, after recovery, GABA content returned to the control level, but 5-HT did not, indicating that only the serotonergic system was persistently disrupted. Overall, our results suggest that the disruption of the serotonergic system and oxidative stress may aggravate neurotoxicity and that the former was the main reason for the depressive-like behavior. This study could help to unravel the mechanisms of the behavioral alterations induced by early-stage THCP exposure in zebrafish.

APA, Harvard, Vancouver, ISO, and other styles

20

Bui Thi, Ngoc Hieu, Ngoc Anh Nguyen Thi, Gilbert Audira, Petrus Siregar, Sung-Tzu Liang, Jong-Chin Huang, and Chung-Der Hsiao. "Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish." International Journal of Molecular Sciences 21, no. 5 (March 7, 2020): 1844. http://dx.doi.org/10.3390/ijms21051844.

Full text

Abstract:

Lead and lead-derived compounds have been extensively utilized in industry, and their chronic toxicity towards aquatic animals has not been thoroughly addressed at a behavioral level. In this study, we assessed the risk of exposure to lead at a waterborne environmental concentration in adult zebrafish by behavioral and biochemical analyses. Nine tests, including three-dimension (3D) locomotion, novel tank exploration, mirror biting, predator avoidance, social interaction, shoaling, circadian rhythm locomotor activity, color preference, and a short-term memory test, were performed to assess the behavior of adult zebrafish after the exposure to 50 ppb PbCl2 for one month. The brain tissues were dissected and subjected to biochemical assays to measure the relative expression of stress biomarkers and neurotransmitters to elucidate the underlying mechanisms for behavioral alterations. The results of the behavioral tests showed that chronic exposure to lead could elevate the stress and anxiety levels characterized by elevated freezing and reduced exploratory behaviors. The chronic exposure to PbCl2 at a low concentration also induced a sharp reduction of aggressiveness and short-term memory. However, no significant change was found in predator avoidance, social interaction, shoaling, or color preference. The biochemical assays showed elevated cortisol and reduced serotonin and melatonin levels in the brain, thus, altering the behavior of the PbCl2-exposed zebrafish. In general, this study determined the potential ecotoxicity of long-term lead exposure in adult zebrafish through multiple behavioral assessments. The significant findings were that even at a low concentration, long-term exposure to lead could impair the memory and cause a decrease in the aggressiveness and exploratory activities of zebrafish, which may reduce their survival fitness.

APA, Harvard, Vancouver, ISO, and other styles

21

KÖKTÜRK, Mine, Aybek YİĞİT, and Ekrem SULUKAN. "Green Synthesis Iron Oxide Nanoparticles (Fe@AV NPs) Induce Developmental Toxicity and Anxiety-Like Behavior in Zebrafish Embryo-Larvae." Marine Science and Technology Bulletin 12, no. 1 (March 31, 2023): 39–50. http://dx.doi.org/10.33714/masteb.1220668.

Full text

Abstract:

The synthesis of nanoparticles and the usage areas of these nanoparticles show a rapid increase. In addition to the beneficial use of nanoparticles, their toxic effects cannot be ignored. In our study, iron oxide nanoparticle (Fe@AV NPs) (mean size: 20.852 nm) was synthesized from Aloe vera plant and the developmental toxicity of zebrafish was investigated. Zebrafish embryo-larvae were treated with different concentrations of Fe@AV NPs (1, 10, and 50 mg/L) starting at 4 hours after fertilization and continuing until 96 hours, and different developmental parameters (such as survival rate, hatchability rates, malformations, and behavior) were examined. In our study, it was determined that Fe@AV NPs caused developmental toxicity in zebrafish embryos depending on the dose increase. More than 60% died at 96 hours, especially in the highest (50 mg/L) application group. It was observed that Fe@AV NPs decreased and delayed the success of exiting the chorion depending on the dose increase, and caused various morphological abnormalities (like pericardial edema, tail deformation, and scoliosis) in all application groups except the lowest application group (1 mg/L). While 10 mg/L Fe@AV NPs caused sleep-like behaviors during the daytime by decreasing the daytime motility of the larvae, it caused hyperactivity by increasing their nocturnal motility. The results of thigmotaxis, which is an anxiety parameter, were found to increase anxiety at 10 mg/L Fe@AV NPs exposure.Our findings showed that Fe@AV NPs synthesized from Aloe vera plant have in vivo toxicity and their use at concentrations lower than 1 mg/L can be safe in environmental and medical applications.

APA, Harvard, Vancouver, ISO, and other styles

22

Calvo-Ochoa, Erika, and Christine Byrd-Jacobs. "The Olfactory System of Zebrafish as a Model for the Study of Neurotoxicity and Injury: Implications for Neuroplasticity and Disease." International Journal of Molecular Sciences 20, no. 7 (April 2, 2019): 1639. http://dx.doi.org/10.3390/ijms20071639.

Full text

Abstract:

The olfactory system, composed of the olfactory organs and the olfactory bulb, allows organisms to interact with their environment and through the detection of odor signals. Olfaction mediates behaviors pivotal for survival, such as feeding, mating, social behavior, and danger assessment. The olfactory organs are directly exposed to the milieu, and thus are particularly vulnerable to damage by environmental pollutants and toxicants, such as heavy metals, pesticides, and surfactants, among others. Given the widespread occurrence of olfactory toxicants, there is a pressing need to understand the effects of these harmful compounds on olfactory function. Zebrafish (Danio rerio) is a valuable model for studying human physiology, disease, and toxicity. Additionally, the anatomical components of the zebrafish olfactory system are similar to those of other vertebrates, and they present a remarkable degree of regeneration and neuroplasticity, making it an ideal model for the study of regeneration, reorganization and repair mechanisms following olfactory toxicant exposure. In this review, we focus on (1) the anatomical, morphological, and functional organization of the olfactory system of zebrafish; (2) the adverse effects of olfactory toxicants and injury to the olfactory organ; and (3) remodeling and repair neuroplasticity mechanisms following injury and degeneration by olfactory toxicant exposure.

APA, Harvard, Vancouver, ISO, and other styles

23

Guillén, Amaimen, Yeferzon Ardila, Mabel Juliana Noguera, Ana Lucía Campaña, Miranda Bejarano, Veronica Akle, and Johann F. Osma. "Toxicity of Modified Magnetite-Based Nanocomposites Used for Wastewater Treatment and Evaluated on Zebrafish (Danio rerio) Model." Nanomaterials 12, no. 3 (January 29, 2022): 489. http://dx.doi.org/10.3390/nano12030489.

Full text

Abstract:

Magnetite-based nanocomposites are used for biomedical, industrial, and environmental applications. In this study, we evaluated their effects on survival, malformation, reproduction, and behavior in a zebrafish animal model. Nanoparticles were synthesized by chemical coprecipitation and were surface-functionalized with (3-aminopropyl) triethoxysilane (APTES), L-cysteine (Cys), and 3-(triethoxysilyl) propylsuccinic anhydride (CAS). All these nanocomposites were designed for the treatment of wastewater. Zebrafish embryos at 8 h post-fertilization (hpf) and larvae at 4 days post-fertilization (dpf) were exposed to the magnetic nanocomposites Fe3O4 MNP (magnetite), MNP+APTES, MNP+Cys, MNP+APTES+Cys, and MNP+CAS, at concentrations of 1, 10, 100, and 1000 µg/mL. Zebrafish were observed until 13 dpf, registering daily hatching, survival, and malformations. Behavior was tested at 10 dpf for larvae, and reproduction was analyzed later in adulthood. The results showed that the toxicity of the nanocomposites used were relatively low. Exploratory behavior tests showed no significant changes. Reproduction in adults treated during development was not affected, even at concentrations above the OECD recommendation. Given the slight effects observed so far, these results suggest that nanocomposites at the concentrations evaluated here could be a viable alternative for water remediation because they do not affect the long-term survival and welfare of the animals.

APA, Harvard, Vancouver, ISO, and other styles

24

Qiu, Xuchun, Michaela Sia Tengbe, Xingyi Xia, Kejun Dong, Chen Chen, Yanhong Shi, Ming Li, Hai Xu, Xiangyang Wu, and Kun Chen. "Impacts of Cetylpyridinium Chloride on the Survival, Development, Behavior, and Oxidative Stress of Early-Life-Stage Zebrafish (Danio rerio)." Antioxidants 11, no. 4 (March 30, 2022): 676. http://dx.doi.org/10.3390/antiox11040676.

Full text

Abstract:

Cetylpyridinium chloride (CPC) is a widely used surfactant that has been detected in various water ecosystems. However, knowledge on the toxicity of CPC to fish remains scarce. Here, we examined the survival, development, behavior, and oxidative stress in the early life stages of zebrafish exposed to CPC (0, 4, 40, 400, and 1200 μg/L) until 120 h post-fertilization (hpf). Results showed that CPC induced significant mortality at 400 and 1200 μg/L, with a 120 h-EC50 value of 175.9 μg/L. CPC significantly decreased the heart rate of embryos (48 hpf; 4–400 μg/L) and larvae (72 hpf; 40 and 400 μg/L). At 120 hpf, CPC exhibited a dual effect on the locomotion activity (decreased at 400 μg/L and increased at 4 and 40 μg/L) and elevated the reactive oxygen species, superoxide dismutase, and glutathione levels in zebrafish larvae at 400 µg/L. In addition, a correlation analysis revealed that CPC-induced oxidative stress might play a critical role in mediating the cardiac and behavioral toxicity of CPC to zebrafish larvae. Our findings suggest that CPC may disturb the fish’s development, behavior, and oxidative status at environmentally relevant concentrations, which should not be ignored when assessing its potential risks to aquatic ecosystems.

APA, Harvard, Vancouver, ISO, and other styles

25

Haque, Enamul, and Alister Ward. "Zebrafish as a Model to Evaluate Nanoparticle Toxicity." Nanomaterials 8, no. 7 (July 23, 2018): 561. http://dx.doi.org/10.3390/nano8070561.

Full text

Abstract:

Nanoparticles are increasingly being developed for in vivo use, from targeted drug delivery to diagnostics, where they have enormous potential, while they are also being used for a variety of applications that can result in environmental exposure for humans. Understanding how specific nanoparticles interact with cells and cell systems is essential to gauge their safety with respect to either clinical or environmental exposure. Zebrafish is being increasingly employed as a model to evaluate nanoparticle biocompatibility. This review describes this model and how it can be used to assess nanoparticle toxicity at multiple levels, including mortality, teratogenicity, immunotoxicity, genotoxicity, as well as alterations in reproduction, behavior and a range of other physiological readouts. This review also provides an overview of studies using this model to assess the toxicity of metal, metal oxide and carbon-based nanoparticles. It is anticipated that this information will inform research aimed at developing biocompatible nanoparticles for a range of uses.

APA, Harvard, Vancouver, ISO, and other styles

26

Sarasamma, Sreeja, Gilbert Audira, Petrus Siregar, Nemi Malhotra, Yu-Heng Lai, Sung-Tzu Liang, Jung-Ren Chen, Kelvin H. C. Chen, and Chung-Der Hsiao. "Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure." International Journal of Molecular Sciences 21, no. 4 (February 19, 2020): 1410. http://dx.doi.org/10.3390/ijms21041410.

Full text

Abstract:

Plastic pollution is a growing global emergency and it could serve as a geological indicator of the Anthropocene era. Microplastics are potentially more hazardous than macroplastics, as the former can permeate biological membranes. The toxicity of microplastic exposure on humans and aquatic organisms has been documented, but the toxicity and behavioral changes of nanoplastics (NPs) in mammals are scarce. In spite of their small size, nanoplastics have an enormous surface area, which bears the potential to bind even bigger amounts of toxic compounds in comparison to microplastics. Here, we used polystyrene nanoplastics (PS-NPs) (diameter size at ~70 nm) to investigate the neurobehavioral alterations, tissue distribution, accumulation, and specific health risk of nanoplastics in adult zebrafish. The results demonstrated that PS-NPs accumulated in gonads, intestine, liver, and brain with a tissue distribution pattern that was greatly dependent on the size and shape of the NPs particle. Importantly, an analysis of multiple behavior endpoints and different biochemical biomarkers evidenced that PS-NPs exposure induced disturbance of lipid and energy metabolism as well as oxidative stress and tissue accumulation. Pronounced behavior alterations in their locomotion activity, aggressiveness, shoal formation, and predator avoidance behavior were exhibited by the high concentration of the PS-NPs group, along with the dysregulated circadian rhythm locomotion activity after its chronic exposure. Moreover, several important neurotransmitter biomarkers for neurotoxicity investigation were significantly altered after one week of PS-NPs exposure and these significant changes may indicate the potential toxicity from PS-NPs exposure. In addition, after ~1-month incubation, the fluorescence spectroscopy results revealed the accumulation and distribution of PS-NPs across zebrafish tissues, especially in gonads, which would possibly further affect fish reproductive function. Overall, our results provided new evidence for the adverse consequences of PS-NPs-induced behavioral dysregulation and changes at the molecular level that eventually reduce the survival fitness of zebrafish in the ecosystem.

APA, Harvard, Vancouver, ISO, and other styles

27

Hwang, Kyu-Seok, Yuji Son, Seong Soon Kim, Dae-Seop Shin, So Hee Lim, Jung Yoon Yang, Ha Neul Jeong, Byung Hoi Lee, and Myung Ae Bae. "Size-Dependent Effects of Polystyrene Nanoparticles (PS-NPs) on Behaviors and Endogenous Neurochemicals in Zebrafish Larvae." International Journal of Molecular Sciences 23, no. 18 (September 14, 2022): 10682. http://dx.doi.org/10.3390/ijms231810682.

Full text

Abstract:

Microplastics, small pieces of plastic derived from polystyrene, have recently become an ecological hazard due to their toxicity and widespread occurrence in aquatic ecosystems. In this study, we exposed zebrafish larvae to two types of fluorescent polystyrene nanoparticles (PS-NPs) to identify their size-dependent effects. PS-NPs of 50 nm, unlike 100 nm PS-NPs, were found to circulate in the blood vessels and accumulate in the brains of zebrafish larvae. Behavioral and electroencephalogram (EEG) analysis showed that 50 nm PS-NPs induce abnormal behavioral patterns and changes in EEG power spectral densities in zebrafish larvae. In addition, the quantification of endogenous neurochemicals in zebrafish larvae showed that 50 nm PS-NPs disturb dopaminergic metabolites, whereas 100 nm PS-NPs do not. Finally, we assessed the effect of PS-NPs on the permeability of the blood–brain barrier (BBB) using a microfluidic system. The results revealed that 50 nm PS-NPs have high BBB penetration compared with 100 nm PS-NPs. Taken together, we concluded that small nanoparticles disturb the nervous system, especially dopaminergic metabolites.

APA, Harvard, Vancouver, ISO, and other styles

28

LeFauve, Matthew K., Roxanne Bérubé, Samantha Heldman, Yu-Ting Tiffany Chiang, and Christopher D. Kassotis. "Cetyl Alcohol Polyethoxylates Disrupt Metabolic Health in Developmentally Exposed Zebrafish." Metabolites 13, no. 3 (February 28, 2023): 359. http://dx.doi.org/10.3390/metabo13030359.

Full text

Abstract:

Alcohol polyethoxylates (AEOs), such as cetyl alcohol ethoxylates (CetAEOs), are high-production-volume surfactants used in laundry detergents, hard-surface cleaners, pesticide formulations, textile production, oils, paints, and other products. AEOs have been suggested as lower toxicity replacements for alkylphenol polyethoxylates (APEOs), such as the nonylphenol and octylphenol polyethoxylates. We previously demonstrated that nonylphenol polyethoxylates induced triglyceride accumulation in several in vitro adipogenesis models and promoted adiposity and increased body weights in developmentally exposed zebrafish. We also demonstrated that diverse APEOs and AEOs were able to increase triglyceride accumulation and/or pre-adipocyte proliferation in a murine pre-adipocyte model. As such, the goals of this study were to assess the potential of CetAEOs to promote adiposity and alter growth and/or development (toxicity, length, weight, behavior, energy expenditure) of developmentally exposed zebrafish (Danio rerio). We also sought to expand our understanding of ethoxylate chain-length dependent effects through interrogation of varying chain-length CetAEOs. We demonstrated consistent adipogenic effects in two separate human bone-marrow-derived mesenchymal stem cell models as well as murine pre-adipocytes. Immediately following chemical exposures in zebrafish, we reported disrupted neurodevelopment and aberrant behavior in light/dark activity testing, with medium chain-length CetAEO-exposed fish exhibiting hyperactivity across both light and dark phases. By day 30, we demonstrated that cetyl alcohol and CetAEOs disrupted adipose deposition in developmentally exposed zebrafish, despite no apparent impacts on standard length or gross body weight. This research suggests metabolic health concerns for these common environmental contaminants, suggesting further need to assess molecular mechanisms and better characterize environmental concentrations for human health risk assessments.

APA, Harvard, Vancouver, ISO, and other styles

29

Zizioli, Daniela, Isabella Zanella, Luca Mignani, Melania Degli Antoni, Francesco Castelli, and Eugenia Quiros-Roldan. "Cabotegravir Exposure of Zebrafish (Danio rerio) Embryos Impacts on Neurodevelopment and Behavior." International Journal of Molecular Sciences 24, no. 3 (January 19, 2023): 1994. http://dx.doi.org/10.3390/ijms24031994.

Full text

Abstract:

As most new medications, Cabotegravir (CAB) was recently approved as an antiretroviral treatment of HIV infection without in-depth safety information on in utero exposure. Although no developmental toxicity in rats and rabbits was reported, recent studies demonstrated that CAB decreases pluripotency of human embryonic stem cells. CAB exposure effects during development were assessed in zebrafish embryos by the Fish Embryo Toxicity test after exposure at subtherapeutic concentrations up to 25× the human Cmax. Larvae behavior was assessed by the light–dark locomotion test. The expression of factors involved in neurogenesis was evaluated by whole-mount in situ hybridization. CAB did not cause gross morphological defects at low doses, although pericardial edema, uninflated swim bladder, decreased heartbeats, growth delay, and decreased hatching rate were observed at the highest concentrations. Decreased locomotion was observed even at the subtherapeutic dose, suggesting alterations of nervous system integrity. This hypothesis was supported by the observation of decreased expression of crucial factors involved in early neuronal differentiation in diencephalic and telencephalic dopaminergic areas, midbrain/hindbrain boundary, and craniofacial ganglia. These findings support CAB effects on neurogenesis in zebrafish embryos and suggest long-term follow-up of exposed infants to provide data on drug safety during pregnancy.

APA, Harvard, Vancouver, ISO, and other styles

30

Malhotra, Nemi, Jung-Ren Chen, Sreeja Sarasamma, Gilbert Audira, Petrus Siregar, Sung-Tzu Liang, Yu-Heng Lai, Geng-Ming Lin, Tzong-Rong Ger, and Chung-Der Hsiao. "Ecotoxicity Assessment of Fe3O4 Magnetic Nanoparticle Exposure in Adult Zebrafish at an Environmental Pertinent Concentration by Behavioral and Biochemical Testing." Nanomaterials 9, no. 6 (June 9, 2019): 873. http://dx.doi.org/10.3390/nano9060873.

Full text

Abstract:

Magnetic Nanoparticles (MNPs) are widely being investigated as novel promising multifunctional agents, specifically in the fields of development for theranostics, electronics, waste water treatment, cosmetics, and energy storage devices. Unique, superior, and indispensable properties of magnetization, heat transfer, and melting temperature make MNPs emerge in the field of therapeutics in future healthcare industries. However, MNPs ecotoxicity as well as behavioral toxicity is still unexplored. Ecotoxicity analysis may assist investigate MNPs uptake mechanism and its influence on bioavailability under a given set of environmental factors, which can be followed to investigate the biomagnification of MNPs in the environment and health risk possessed by them in an ecological food chain. In this study, we attempted to determine the behavioral changes in zebrafishes at low (1 ppm) or high (10 ppm) concentration levels of Fe3O4 MNPs. The synthesized Fe3O4 MNPs sized at 15 nm were characterized by the transmission electron microscope (TEM), the superconducting quantum interference device (SQUID) magnetometer, and the multiple behavior tests for novel tank, mirror biting, conspecific social interaction, shoaling, circadian rhythm, and short-term memory of zebrafish under MNPs chronic exposure were demonstrated. Low concentration MNP exposure did not trigger alteration for majority behavioral and biochemical tests in adult zebrafish. However, tight shoal groups were observed at a high concentration of MNPs exposure along with a modest reduction in fish exploratory behavior and a significant reduction in conspecific social interaction behavior. By using enzyme-linked immunosorbent assays (ELISA), we found a high dose of MNPs exposure significantly elevated cortisol, acetylcholine, and catalase levels while reducing serotonin, acetylcholine esterase, and dopamine levels in the brain. Our data demonstrates chronic MNPs exposure at an environmentally-relevant dose is relatively safe by supporting evidence from an array of behavioral and biochemical tests. This combinational approach using behavioral and biochemical tests would be helpful for understanding the MNPs association with anticipated colloids and particles effecting bioavailability and uptake into cells and organisms.

APA, Harvard, Vancouver, ISO, and other styles

31

Ferreira, KdeS, L. Tachibana, DRdaS Colombo, SCOda Paixão, CM Ferreira, and C. Badaró-Pedroso. "Toxic effects in Aphanomyces brasiliensis and zebrafish embryos caused by oomyceticides." Diseases of Aquatic Organisms 144 (April 8, 2021): 75–87. http://dx.doi.org/10.3354/dao03575.

Full text

Abstract:

In fish farming, high losses occur during egg incubation and larviculture due to diseases caused by oomycetes. This study aimed to identify the oomycete species that occurs in zebrafish Danio rerio eggs and to evaluate the oomyceticidal effect of copper sulfate, bronopol and methylene blue on the mycelial growth of this organism, as well as to determine the lethal and sublethal toxicity of these compounds in embryos of D. rerio. The isolates were cultivated in yeast-starch medium to determine the concentration necessary to inhibit mycelial growth by 50% (IC50) and 100% (minimum oomyceticidal concentration) after a 96 h exposure to these compounds. In addition, tests with D. rerio eggs were conducted to determine the lethal concentrations for 50% of the organisms (96h-LC50), and the concentrations that inhibited 17% of the eggs hatching (96h-IC17) after 96 h. The organism responsible for the mortality of D. rerio eggs was classified by classical and molecular methods as Aphanomyces brasiliensis, representing the first report of this pathogen in zebrafish eggs. IC50 values could be determined for both bronopol and copper sulfate, whereas methylene blue had low effectiveness against the oomycete. Copper showed high toxicity to D. rerio eggs at low concentrations, while methylene blue and bronopol toxicity was low and similar to each other. The use of bronopol at a concentration of 4.8 mg l-1 for the treatment of zebrafish eggs allows controlling the pathology without causing deleterious effects to the treated organisms.

APA, Harvard, Vancouver, ISO, and other styles

32

Qin, Yingju, Xiaohong Wang, Xiliang Yan, Di Zhu, Jia Wang, Siying Chen, Shuo Wang, Yang Wen, Christopher J. Martyniuk, and Yuanhui Zhao. "Developmental toxicity of fenbuconazole in zebrafish: Effects on mitochondrial respiration and locomotor behavior." Toxicology 470 (March 2022): 153137. http://dx.doi.org/10.1016/j.tox.2022.153137.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Abu Bakar, Noraini, Wan Norhamidah Wan Ibrahim, Che Azurahanim Che Abdullah, Nurul Farhana Ramlan, Khozirah Shaari, Shamarina Shohaimi, Ahmed Mediani, Nurrul Shaqinah Nasruddin, Cheol-Hee Kim, and Siti Munirah Mohd Faudzi. "Embryonic Arsenic Exposure Triggers Long-Term Behavioral Impairment with Metabolite Alterations in Zebrafish." Toxics 10, no. 9 (August 24, 2022): 493. http://dx.doi.org/10.3390/toxics10090493.

Full text

Abstract:

Arsenic trioxide (As2O3) is a ubiquitous heavy metal in the environment. Exposure to this toxin at low concentrations is unremarkable in developing organisms. Nevertheless, understanding the underlying mechanism of its long-term adverse effects remains a challenge. In this study, embryos were initially exposed to As2O3 from gastrulation to hatching under semi-static conditions. Results showed dose-dependent increased mortality, with exposure to 30–40 µM As2O3 significantly reducing tail-coiling and heart rate at early larval stages. Surviving larvae after 30 µM As2O3 exposure showed deficits in motor behavior without impairment of anxiety-like responses at 6 dpf and a slight impairment in color preference behavior at 11 dpf, which was later evident in adulthood. As2O3 also altered locomotor function, with a loss of directional and color preference in adult zebrafish, which correlated with changes in transcriptional regulation of adsl, shank3a, and tsc1b genes. During these processes, As2O3 mainly induced metabolic changes in lipids, particularly arachidonic acid, docosahexaenoic acid, prostaglandin, and sphinganine-1-phosphate in the post-hatching period of zebrafish. Overall, this study provides new insight into the potential mechanism of arsenic toxicity leading to long-term learning impairment in zebrafish and may benefit future risk assessments of other environmental toxins of concern.

APA, Harvard, Vancouver, ISO, and other styles

34

Vranic, Sandra, Yasuhito Shimada, Sahoko Ichihara, Masayuki Kimata, Wenting Wu, Toshio Tanaka, Sonja Boland, Lang Tran, and Gaku Ichihara. "Toxicological Evaluation of SiO2 Nanoparticles by Zebrafish Embryo Toxicity Test." International Journal of Molecular Sciences 20, no. 4 (February 18, 2019): 882. http://dx.doi.org/10.3390/ijms20040882.

Full text

Abstract:

As the use of nanoparticles (NPs) is increasing, the potential toxicity and behavior of NPs in living systems need to be better understood. Our goal was to evaluate the developmental toxicity and bio-distribution of two different sizes of fluorescently-labeled SiO2 NPs, 25 and 115 nm, with neutral surface charge or with different surface functionalization, rendering them positively or negatively charged, in order to predict the effect of NPs in humans. We performed a zebrafish embryo toxicity test (ZFET) by exposing the embryos to SiO2 NPs starting from six hours post fertilization (hpf). Survival rate, hatching time, and gross morphological changes were assessed at 12, 24, 36, 48, 60, and 72 hpf. We evaluated the effect of NPs on angiogenesis by counting the number of sub-intestinal vessels between the second and seventh intersegmental vessels and gene expression analysis of vascular endothelial growth factor (VEGF) and VEGF receptors at 72 hpf. SiO2 NPs did not show any adverse effects on survival rate, hatching time, gross morphology, or physiological angiogenesis. We found that SiO2 NPs were trapped by the chorion up until to the hatching stage. After chemical removal of the chorion (dechorionation), positively surface-charged SiO2 NPs (25 nm) significantly reduced the survival rate of the fish compared to the control group. These results indicate that zebrafish chorion acts as a physical barrier against SiO2 NPs, and removing the chorions in ZFET might be necessary for evaluation of toxicity of NPs.

APA, Harvard, Vancouver, ISO, and other styles

35

Kulakkattolickal, Augusthy Thevasia, and Donald L. Kramer. "Effect of Surface Access and Oxygen Concentration on the Toxicity of Croton tiglium (Euphorbiaceae) Seed Extract to Brachydanio rerio (Cyprinidae)." Canadian Journal of Fisheries and Aquatic Sciences 44, no. 7 (July 1, 1987): 1358–61. http://dx.doi.org/10.1139/f87-161.

Full text

Abstract:

Zebrafish (Brachydanio rerio) (Cyprinidae) were exposed to a toxic extract of Croton tiglium (Euphorbiaceae) seeds (4 mg∙L−1) at 0.8, 1.1 and 8.1 mg O2∙L−1, with and without surface access. Equilibrium loss occurred more quickly at lower dissolved oxygen concentrations. At 0.8 and 1.1 mg O2∙L−1, equilibrium loss also occurred more quickly when access to the water surface was denied. Surface access is therefore an important factor in studies of the interaction between toxicity and dissolved oxygen concentration. We suggest that, by performing aquatic surface respiration, zebrafish with surface access in hypoxic water reduce their ventilation rate and hence their uptake rate of toxins.

APA, Harvard, Vancouver, ISO, and other styles

36

de Souza Freire, Ingrid, Maria Luiza Fascineli, Tathyana Benetis Piau, Bruno Fiorelini Pereira, and Cesar Koppe Grisolia. "Multilevel Toxicity Evaluations of Polyethylene Microplastics in Zebrafish (Danio rerio)." International Journal of Environmental Research and Public Health 20, no. 4 (February 17, 2023): 3617. http://dx.doi.org/10.3390/ijerph20043617.

Full text

Abstract:

Microplastics in freshwater environments pose a serious threat to living beings. Polyethylene microplastics (PE-MP) are the type most used around the world as microbeads in personal care products, and they have been found in aquatic organisms. The behavior and toxicity of fluorescent PE-MP spheres with an average diameter of 58.9 μm were studied in adult, juvenile and embryo zebrafish (Danio rerio). The adults were studied for genotoxicity, cytotoxicity, histology and biochemical markers. Juveniles underwent a follow-up in the gastrointestinal (GI) tract with histologic observations, and embryos were studied for embryotoxicity with the FET-test. In adults, micronucleus test and comet assays found neither genotoxicity after acute exposure for 96 h at concentrations of 0.0, 12.5, 50 and 100 mg.L−1, nor cytotoxicity through the nuclear abnormalities test. Acetylcholinesterase (AChE), Glutathione-S-Transferase (GST) and Lactate Dehydrogenase (LDH) activities were measured in adults exposed for 96 h. The AChE and GST activities were significantly changed, while no changes occurred for LDH. In conclusion, these PE-MP spheres did not cause serious toxic effects in zebrafish because there was no internalization. The observed biochemical changes in AChE and GST may be associated with GI microbiological dysbiosis, previously reported. The PE-MP spheres in the intestine of juveniles remained present for 12–15 days on average after the post-exposure clearance study, showing a slow depuration. The histological analysis, in adults, found no internalization of these microbeads, with complete depuration. The PE-MP spheres did not cross the chorion barrier, showing no embryotoxic effects after exposures at 0.0, 6.25, 12.5, 50.0 or 100.0 mg.L−1 for 96 h.

APA, Harvard, Vancouver, ISO, and other styles

37

Chen, Zi-Yu, Nian-Jhen Li, Fong-Yu Cheng, Jian-Feng Hsueh, Chiao-Ching Huang, Fu-I. Lu, Tzu-Fun Fu, Shian-Jang Yan, Yu-Hsuan Lee, and Ying-Jan Wang. "The Effect of the Chorion on Size-Dependent Acute Toxicity and Underlying Mechanisms of Amine-Modified Silver Nanoparticles in Zebrafish Embryos." International Journal of Molecular Sciences 21, no. 8 (April 20, 2020): 2864. http://dx.doi.org/10.3390/ijms21082864.

Full text

Abstract:

As the worldwide application of nanomaterials in commercial products increases every year, various nanoparticles from industry might present possible risks to aquatic systems and human health. Presently, there are many unknowns about the toxic effects of nanomaterials, especially because the unique physicochemical properties of nanomaterials affect functional and toxic reactions. In our research, we sought to identify the targets and mechanisms for the deleterious effects of two different sizes (~10 and ~50 nm) of amine-modified silver nanoparticles (AgNPs) in a zebrafish embryo model. Fluorescently labeled AgNPs were taken up into embryos via the chorion. The larger-sized AgNPs (LAS) were distributed throughout developing zebrafish tissues to a greater extent than small-sized AgNPs (SAS), which led to an enlarged chorion pore size. Time-course survivorship revealed dose- and particle size-responsive effects, and consequently triggered abnormal phenotypes. LAS exposure led to lysosomal activity changes and higher number of apoptotic cells distributed among the developmental organs of the zebrafish embryo. Overall, AgNPs of ~50 nm in diameter exhibited different behavior from the ~10-nm-diameter AgNPs. The specific toxic effects caused by these differences in nanoscale particle size may result from the different mechanisms, which remain to be further investigated in a follow-up study.

APA, Harvard, Vancouver, ISO, and other styles

38

Sarasamma, Sreeja, Gilbert Audira, Prabu Samikannu, Stevhen Juniardi, Petrus Siregar, Erwei Hao, Jung-Ren Chen, and Chung-Der Hsiao. "Behavioral Impairments and Oxidative Stress in the Brain, Muscle, and Gill Caused by Chronic Exposure of C70 Nanoparticles on Adult Zebrafish." International Journal of Molecular Sciences 20, no. 22 (November 18, 2019): 5795. http://dx.doi.org/10.3390/ijms20225795.

Full text

Abstract:

There is an imperative need to develop efficient whole-animal-based testing assays to determine the potential toxicity of engineered nanomaterials. While previous studies have demonstrated toxicity in lung and skin cells after C70 nanoparticles (NPs) exposure, the potential detrimental role of C70 NPs in neurobehavior is largely unaddressed. Here, we evaluated the chronic effects of C70 NPs exposure on behavior and alterations in biochemical responses in adult zebrafish. Two different exposure doses were used for this experiment: low dose (0.5 ppm) and high dose (1.5 ppm). Behavioral tests were performed after two weeks of exposure of C70 NPs. We found decreased locomotion, exploration, mirror biting, social interaction, and shoaling activities, as well as anxiety elevation and circadian rhythm locomotor activity impairment after ~2 weeks in the C70 NP-exposed fish. The results of biochemical assays reveal that following exposure of zebrafish to 1.5 ppm of C70 NPs, the activity of superoxide dismutase (SOD) in the brain and muscle tissues increased significantly. In addition, the concentration of reactive oxygen species (ROS) also increased from 2.95 ± 0.12 U/ug to 8.46 ± 0.25 U/ug and from 0.90 ± 0.03 U/ug to 3.53 ± 0.64 U/ug in the muscle and brain tissues, respectively. Furthermore, an increased level of cortisol was also observed in muscle and brain tissues, ranging from 17.95 ± 0.90 pg/ug to 23.95 ± 0.66 pg/ug and from 3.47 ± 0.13 pg/ug to 4.91 ± 0.51 pg/ug, respectively. Increment of Hif1-α level was also observed in both tissues. The elevation was ranging from 11.65 ± 0.54 pg/ug to 18.45 ± 1.00 pg/ug in the muscle tissue and from 4.26 ± 0.11 pg/ug to 6.86 ± 0.37 pg/ug in the brain tissue. Moreover, the content of DNA damage and inflammatory markers such as ssDNA, TNF-α, and IL-1β were also increased substantially in the brain tissues. Significant changes in several biomarker levels, including catalase and malondialdehyde (MDA), were also observed in the gill tissues. Finally, we used a neurophenomic approach with a particular focus on environmental influences, which can also be easily adapted for other aquatic fish species, to assess the toxicity of metal and carbon-based nanoparticles. In summary, this is the first study to illustrate the adult zebrafish toxicity and the alterations in several neurobehavior parameters after zebrafish exposure to environmentally relevant amounts of C70 NPs.

APA, Harvard, Vancouver, ISO, and other styles

39

Liao, Gengze, Peijun Wang, Jiawei Zhu, Xueqiong Weng, Shanshan Lin, Jingwen Huang, Yongjie Xu, et al. "Joint toxicity of lead and cadmium on the behavior of zebrafish larvae: An antagonism." Aquatic Toxicology 238 (September 2021): 105912. http://dx.doi.org/10.1016/j.aquatox.2021.105912.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Li, Meng, XingYu Liu, and XiZeng Feng. "Cardiovascular toxicity and anxiety-like behavior induced by deltamethrin in zebrafish (Danio rerio) larvae." Chemosphere 219 (March 2019): 155–64. http://dx.doi.org/10.1016/j.chemosphere.2018.12.011.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Huang, Haihua, Changjiang Huang, Lijun Wang, Xiaowei Ye, Chenglian Bai, Michael T. Simonich, Robert L. Tanguay, and Qiaoxiang Dong. "Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid (PFOS)." Aquatic Toxicology 98, no. 2 (June 2010): 139–47. http://dx.doi.org/10.1016/j.aquatox.2010.02.003.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Licitra, Rosario, Marco Martinelli, Luigi Petrocchi Jasinski, Maria Marchese, Claudia Kiferle, and Baldassare Fronte. "In Vivo Evaluation of Cannabis sativa Full Extract on Zebrafish Larvae Development, Locomotion Behavior and Gene Expression." Pharmaceuticals 14, no. 12 (November 25, 2021): 1224. http://dx.doi.org/10.3390/ph14121224.

Full text

Abstract:

Historically, humans have been using Cannabis sativa for both recreational and medical purposes. Nowadays, cannabis-based products have gained scientific interest due to their beneficial effects on several syndromes and illnesses. The biological activity of cannabinoids is essentially due to the interaction with the endocannabinoid system, and zebrafish (Danio rerio) is a very well-known and powerful in vivo model for studying such specific interactions. The aim of the study was to investigate the effects of different doses of a Cannabis sativa whole extract [dissolved in dimethyl sulfoxide (DMSO)] on zebrafish eggs’ hatchability, embryo post-hatching survival, larvae locomotion behavior and mRNA gene expression. The results showed the absence of toxicity, and no significant differences were observed between treatments for both embryo hatching and survival rate. In addition, larvae exposed to the cannabis extract at the highest dose [containing 1.73 nM and 22.3 nM of ∆9-tetrahydrocannabinol (THC) and cannabidiol (CBD), respectively] showed an increased locomotion compared to the control and DMSO treated groups. Moreover, qRT-PCR analysis showed that the highest dosage of cannabis induced an over-expression of cnr1 and cnr2 cannabinoid receptors. In conclusion, the exposition of zebrafish larvae to the whole extract of Cannabis sativa showed no negative effects on embryo development and survival and enhanced the larvae’s locomotor performances. These findings may open up possible Cannabis sativa applications in human pharmacology as well as in other animal sectors.

APA, Harvard, Vancouver, ISO, and other styles

43

Jarema, Kimberly A., Deborah L. Hunter, Bridgett N. Hill, Jeanene K. Olin, Katy N. Britton, Matthew R. Waalkes, and Stephanie Padilla. "Developmental Neurotoxicity and Behavioral Screening in Larval Zebrafish with a Comparison to Other Published Results." Toxics 10, no. 5 (May 17, 2022): 256. http://dx.doi.org/10.3390/toxics10050256.

Full text

Abstract:

With the abundance of chemicals in the environment that could potentially cause neurodevelopmental deficits, there is a need for rapid testing and chemical screening assays. This study evaluated the developmental toxicity and behavioral effects of 61 chemicals in zebrafish (Danio rerio) larvae using a behavioral Light/Dark assay. Larvae (n = 16–24 per concentration) were exposed to each chemical (0.0001–120 μM) during development and locomotor activity was assessed. Approximately half of the chemicals (n = 30) did not show any gross developmental toxicity (i.e., mortality, dysmorphology or non-hatching) at the highest concentration tested. Twelve of the 31 chemicals that did elicit developmental toxicity were toxic at the highest concentration only, and thirteen chemicals were developmentally toxic at concentrations of 10 µM or lower. Eleven chemicals caused behavioral effects; four chemicals (6-aminonicotinamide, cyclophosphamide, paraquat, phenobarbital) altered behavior in the absence of developmental toxicity. In addition to screening a library of chemicals for developmental neurotoxicity, we also compared our findings with previously published results for those chemicals. Our comparison revealed a general lack of standardized reporting of experimental details, and it also helped identify some chemicals that appear to be consistent positives and negatives across multiple laboratories.

APA, Harvard, Vancouver, ISO, and other styles

44

Jeon, Hwang-Ju, Kyeongnam Kim, Chaeeun Kim, Myoung-Jin Kim, Tae-Oh Kim, and Sung-Eun Lee. "Molecular Mechanisms of Anti-Melanogenic Gedunin Derived from Neem Tree (Azadirachta indica) Using B16F10 Mouse Melanoma Cells and Early-Stage Zebrafish." Plants 10, no. 2 (February 9, 2021): 330. http://dx.doi.org/10.3390/plants10020330.

Full text

Abstract:

Melanogenesis represents a series of processes that produce melanin, a protective skin pigment (against ultraviolet rays), and determines human skin color. Chemicals reducing melanin production have always been in demand in the cosmetic market because of skincare interests, such as whitening. The main mechanism for inhibiting melanin production is the inhibition of tyrosinase (TYR), a key enzyme for melanogenesis. Here, we evaluated gedunin (Ged), a representative limonoid, for its anti-melanogenesis action. Melanin production in vitro was stimulated by alpha-melanocyte stimulating hormone (α-MSH) in B16F10 mouse melanoma cells. Ged reduced α-MSH-stimulated melanin production, inhibiting TYR activity and protein amount. We confirmed this result in vivo in a zebrafish model for melanogenesis. There was no sign of toxicity and malformation of zebrafish embryos during development in all treated concentrations. Ged reduced the number of produced zebrafish embryo pigment dots and melanin contents of embryos. The highly active concentration of Ged (100 µM) was much lower than the positive control, kojic acid (8 mM). Hence, Ged could be a fascinating candidate for anti-melanogenesis reagents.

APA, Harvard, Vancouver, ISO, and other styles

45

Capparucci, Fabiano, Giovanni De Benedetto, Sabrina Natale, Roberta Pecoraro, Carmelo Iaria, and Fabio Marino. "Evaluation of Anaesthetic Effect of Commercial Basil Ocimum basilicum on Zebrafish (Danio rerio) Embryos." Fishes 7, no. 6 (November 3, 2022): 318. http://dx.doi.org/10.3390/fishes7060318.

Full text

Abstract:

Essential oils (EOs) are natural compounds used in various fields such as traditional medicine, gastronomy, and agriculture, often used against plant and animal parasites. In the present study, the toxic and pathological effects of basil (Ocimum basilicum) essential oil on the development of zebrafish embryos was investigated. The manufacturer datasheet reported linalool and eugenol as major compounds. The effects of the essential oil were evaluated through a zebrafish embryo toxicity test (ZFET) following the OECD n°236 guidelines. Embryos were exposed to different essential oil concentrations (50, 100, and 200 μL/L). Results showed mortality effects in the experimental groups in a dose-dependent manner. Moreover, zebrafish exposed to the higher concentration showed several pathological alterations; particularly, delayed hatching, pericardial edema, and a significant reduction of heart rate after 96 h post-fertilization (hpf) compared to the control group. Results reported here suggest an anaesthetic effect of the O. basilicum at low concentrations, due to the possible synergist effect of the main components such as linalool and eugenol which act on the GABAergic system evoking bradycardia.

APA, Harvard, Vancouver, ISO, and other styles

46

Liu, Tuo, Ruilin Wang, Hui Cao, and Aijun Lin. "Polyaspartic acid alleviates heavy metal toxicity in zebrafish (Danio rerio)." Chemistry and Ecology 33, no. 7 (July 20, 2017): 684–93. http://dx.doi.org/10.1080/02757540.2017.1351959.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Gao, Jing, Fang Wang, Jingna Cui, Qiang Zhang, Peng Wang, Donghui Liu, and Zhiqiang Zhou. "Assessment of toxicity and environmental behavior of chiral ethiprole and its metabolites using zebrafish model." Journal of Hazardous Materials 414 (July 2021): 125492. http://dx.doi.org/10.1016/j.jhazmat.2021.125492.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Yan, Wei, Guangyu Li, Qiqi Lu, Jianjun Hou, Meiqi Pan, Maomin Peng, Xitian Peng, Hui Wan, Xixia Liu, and Qin Wu. "Molecular Mechanisms of Tebuconazole Affecting the Social Behavior and Reproduction of Zebrafish." International Journal of Environmental Research and Public Health 20, no. 5 (February 22, 2023): 3928. http://dx.doi.org/10.3390/ijerph20053928.

Full text

Abstract:

The aim of this study was to explore the underlying mechanism of adverse effects caused by tebuconazole (TEB) on the reproduction of aquatic organisms In the present study, in order to explore the effects of TEB on reproduction, four-month-old zebrafish were exposed to TEB (0, DMSO, 0.4 mg/L, 0.8 mg/L, and 1.6 mg/L) for 21 days. After exposure, the accumulations of TEB in gonads were observed and the cumulative egg production was evidently decreased. The decline of fertilization rate in F1 embryos was also observed. Then the changes in sperm motility and histomorphology of gonads were discovered, evaluating that TEB had adverse effects on gonadal development. Additionally, we also found the alternations of social behavior, 17β-estradiol (E2) level, and testosterone (T) level. Furthermore, the expression levels of genes involved in the hypothalamic-pituitary-gonadal (HPG) axis and social behavior were remarkably altered. Taken together, it could be concluded that TEB affected the egg production and fertilization rate by interfering with gonadal development, sex hormone secretion, and social behavior, which were eventually attributed to the disruption of the expressions of genes associated with the HPG axis and social behavior. This study provides a new perspective to understanding the mechanism of TEB-induced reproductive toxicity.

APA, Harvard, Vancouver, ISO, and other styles

49

Tamagno, Wagner Antonio, Carla Alves, Diego Tessaro, Nathália Tafarel Sutorillo, Wallace Santin, and Leonardo José Gil Barcellos. "Deferoxamine Supplementation Abolished Iron-Related Toxicity of Ilex paraguariensis Extract: Behavioral and Biochemical Evaluation in Adult Zebrafish (Danio rerio)." Antioxidants 11, no. 8 (July 31, 2022): 1507. http://dx.doi.org/10.3390/antiox11081507.

Full text

Abstract:

Ilex paraguariensis (Herb mate) is a native plant from South America, widely consumed through the infusion of dried leaves. The presence of antioxidant properties in herb mate may be relevant and contribute to evaluating the effect of its compounds against oxidative stress, which could cause neurodegenerative diseases. Despite having health benefits, there are reports of the presence of heavy metals in extracts obtained from the infusion. One of these metals is iron (Fe), found in large amounts in herb mate. To reverse the cumulative effects of metals and Fe in the body, the use of Deferoxamine (Dfx) is indicated, being a potent chelator of Fe. In this work, we aimed to evaluate the antioxidant potential of the micro-encapsulated extract of I. paraguariensis (MEIP) supplemented with Dfx on zebrafish behavior and biochemical biomarkers. To evaluate the effect per se and the supplementation, four groups were established: the first group was the control (water); the second, fish treated with MEIP; the third group was formed of fish treated with Dfx; while the fourth group was treated with both MEIP and Dfx. When applied alone, Dfx presents an anxiogenic-like pattern on zebrafish (Danio rerio), while the MEIP shows an anxiolytic-like behavior. The antioxidant enzymes are re-modulated close to control when the MEIP + Dfx is applied. The cholinergic system shows an activation of the signaling, as well as the heme radical group formation, which is not affected by the Dfx-chelating effect. Thus, the supplementation of MEIP with Dfx is important to transform this extract into one that is safer and healthier for human consumption.

APA, Harvard, Vancouver, ISO, and other styles

50

Chousidis, Ieremias, Theodoros Chatzimitakos, Constantine Stalikas, and Ioannis Leonardos. "Untargeted Metabolomics Associated with Behavioral and Toxicological Studies Yield Insights into the Impact of 2,6-Dichloro-3-hydroxy-1,4-benzoquinone Disinfection By-Product on Zebrafish Larvae." Fishes 7, no. 6 (December 2, 2022): 368. http://dx.doi.org/10.3390/fishes7060368.

Full text

Abstract:

The disinfection by-product 2,6-dichloro-3-hydroxy-1,4-benzoquinone (2,6-DCBQ-OH) is a halobenzoquinone that emerges after chlorination. Therefore, it will inevitably come into contact with aquatic organisms. The aim of this study was to investigate the effect of 2,6-DCBQ-OH on zebrafish embryos. The dose-dependent toxicity was recorded, and the LC50 value was found to be 186 μg/L. Toxicity was accompanied with morphological, developmental, and behavioral abnormalities, and metabolic alterations. The association of phenotypic alterations with metabolic alterations was investigated through metabolomic study. In the control group, 25 metabolic pathways were identified, and 10 of them remained unaffected upon exposure to the halobenzoquinone. The upregulation of the glutathione pathway suggested that 2,6-DCBQ-OH can cause oxidative stress. In addition, the upregulation of the β-alanine metabolism pathway may be associated with the observed reduced swimming activity observed. Likewise, the downregulation of pathways associated with glycerophospholipids and butyrate can result in endocrine disruption, ineffective regulation of weight and body composition, and glucose homeostasis. The fatty acid elongation and synthesis of essential amino acids are downregulated, which can be associated with insufficient organogenesis at early life stages. In conclusion, exposure of zebrafish to 2,6-DCBQ-OH results in dysregulation and metabolic collapse of the organism, which ultimately result in developmental, morphological, behavioral, and other abnormalities.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Toxicity, behaviour, zebrafish'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles